Predicted mutation
evidence seq id position mutation annotation gene description
RA AP012306 1,076,236 G→A L348F (CTC→TTC)  chaA ← calcium/sodium:proton antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123061,076,2360GA99.5% 1600.3 / ‑2.5 395L348F (CTC→TTC) chaAcalcium/sodium:proton antiporter
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (197/196);  total (198/197)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.09e-01

Only 100 of 406 total aligned reads displayed.

ACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAA  >  AP012306/1076089‑1076382
                                                                                                                                                   |                                                                                                                                                  
aCGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCTGCAAACAGTGCCAGATGCGCTGCGCCATTGAACa                                                                                                                                                  <  2:3635015/150‑1 (MQ=255)
     cTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAACATGTTa                                                                                                                                             <  2:1605669/150‑1 (MQ=255)
       gTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAACATGTTAGt                                                                                                                                           <  1:1383260/150‑1 (MQ=255)
        tCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAACATGTTAGTa                                                                                                                                          >  1:603096/1‑150 (MQ=255)
        tCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAACATGTTAGTa                                                                                                                                          >  2:1716141/1‑150 (MQ=255)
        tCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAACATGTTAGTa                                                                                                                                          >  1:1468063/1‑150 (MQ=255)
           aGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAACATGTTAGTACGt                                                                                                                                       >  2:3300128/1‑150 (MQ=255)
               agtaTGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAACATGTTAGTACGTCCgg                                                                                                                                   >  1:527540/1‑150 (MQ=255)
                gtaTGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAACATGTTAGTACGTCCGGt                                                                                                                                  <  2:685668/150‑1 (MQ=255)
                gtaTGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAACATGTTAGTACGTCCGGt                                                                                                                                  <  2:1512874/150‑1 (MQ=255)
                 taTGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAACATGTTAGTACGTCCGGTg                                                                                                                                 >  2:386804/1‑150 (MQ=255)
                 taTGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAACATGTTAGTACGTCCGGTg                                                                                                                                 >  1:3524084/1‑150 (MQ=255)
                   tGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAACATGTTAGTACGTCCGGTGga                                                                                                                               >  1:2525524/1‑150 (MQ=255)
                   tGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAACATGTTAGTACGTCCGGTGga                                                                                                                               >  2:1004969/1‑150 (MQ=255)
                   tGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAACATGTTAGTACGTCCGGTGga                                                                                                                               >  1:1037090/1‑150 (MQ=255)
                     aagaaATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAACATGTTAGTACGTCCGGTGgaga                                                                                                                             >  2:1082699/1‑150 (MQ=255)
                       gaaATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAACATGTTAGTACGTCCGGTGGAGAAg                                                                                                                           <  1:2963384/150‑1 (MQ=255)
                            aCAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAACATGTTAGTACGTCCGGTGGAGAAGGAGat                                                                                                                      <  1:2336515/150‑1 (MQ=255)
                             cAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAACATGTTAGTACGTCCGGTGGAGAAGGAGata                                                                                                                     >  2:790729/1‑150 (MQ=255)
                               aTAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAACATGTTAGTACGTCCGGTGGAGAAGGAGATATg                                                                                                                   <  2:2626009/150‑1 (MQ=255)
                                   gCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAACATGTTAGTACGTCCGGTGGAGAAGGAGATATGGcac                                                                                                               >  1:1996307/1‑150 (MQ=255)
                                    cAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGAAGCGCTGCGCCATTGAACATGTTAGTACGTCCGGTGGAGAAGGAGATATGGcaca                                                                                                              <  2:1952279/150‑1 (MQ=255)
                                        aaTTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAACATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCAc                                                                                                          >  1:3249821/1‑150 (MQ=255)
                                         aTTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAACATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACt                                                                                                         >  1:1789275/1‑150 (MQ=255)
                                         aTTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAACATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACt                                                                                                         <  1:3864462/150‑1 (MQ=255)
                                          tttATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAACATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTa                                                                                                        >  1:204066/1‑150 (MQ=255)
                                             atatTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAACATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAag                                                                                                     <  1:3065849/150‑1 (MQ=255)
                                             atatTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAACATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAag                                                                                                     <  1:2837030/150‑1 (MQ=255)
                                               attatCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAACATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAagag                                                                                                   >  2:947698/1‑150 (MQ=255)
                                               attatCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAACATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAagag                                                                                                   >  1:89230/1‑150 (MQ=255)
                                               attatCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCCTCATCAAATAGGCGGCAAACAGTGCCAGATGGGCTGAGCCATTGAACATGTTAGTACGTCCGGTGGAGAAGGAGATAGGGCACAGCACTAAagag                                                                                                   >  2:3244608/1‑150 (MQ=255)
                                                ttatCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAACATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAgg                                                                                                  >  2:2168418/1‑150 (MQ=255)
                                                ttatCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAACATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAgg                                                                                                  <  2:3116342/150‑1 (MQ=255)
                                                ttatCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAACATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAgg                                                                                                  >  2:1652059/1‑150 (MQ=255)
                                                 tatCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAACATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGc                                                                                                 >  2:3973908/1‑150 (MQ=255)
                                                  atCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAACATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGcc                                                                                                >  1:3003033/1‑150 (MQ=255)
                                                   tctcCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAACATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGcca                                                                                               <  1:2893080/150‑1 (MQ=255)
                                                      cccccGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAACATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGccacca                                                                                            <  2:3071408/150‑1 (MQ=255)
                                                       ccccGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAACATGTTAGTACGTCCGGCGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCAt                                                                                           >  2:2068495/1‑150 (MQ=255)
                                                          cGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAACATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATcac                                                                                        <  2:2252032/150‑1 (MQ=255)
                                                                ccGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAACATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCAt                                                                                  >  1:2236921/1‑150 (MQ=255)
                                                                  gTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAACATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCAttt                                                                                >  1:1737417/1‑150 (MQ=255)
                                                                  gTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAACATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCAttt                                                                                <  2:3746353/150‑1 (MQ=255)
                                                                  gTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAACATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCAttt                                                                                >  1:1591378/1‑150 (MQ=255)
                                                                       cGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAACATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGgc                                                                           >  2:2426550/1‑150 (MQ=255)
                                                                       cGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGGGCCAGATGCGCTGCGCCATTGAACATGTTAGTACGTCCGGTGGAGAGGGAGATATGGCAGAGCACTAAAGAGGCCACCATCACCACCATTTCTGgc                                                                           >  2:1382752/1‑150 (MQ=255)
                                                                        ggaggaGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAACATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGgcg                                                                          >  2:1284337/1‑150 (MQ=255)
                                                                        ggaggaGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAACATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGgcg                                                                          >  2:1183658/1‑150 (MQ=255)
                                                                         gaggagATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAACATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGgcgc                                                                         >  2:3569998/1‑150 (MQ=255)
                                                                         gaggagATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAACATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGgcgc                                                                         >  1:3192716/1‑150 (MQ=255)
                                                                          aggagATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAACATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCa                                                                        >  1:1515269/1‑150 (MQ=255)
                                                                           ggagATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAACATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCAc                                                                       <  2:2531044/150‑1 (MQ=255)
                                                                           ggagATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAACATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCAc                                                                       <  1:4152235/150‑1 (MQ=255)
                                                                            gagaTATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAACATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCAcc                                                                      >  1:3465305/1‑150 (MQ=255)
                                                                             agaTATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAACATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCa                                                                     >  1:2586570/1‑150 (MQ=255)
                                                                             agaTATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAACATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCa                                                                     >  1:3434548/1‑150 (MQ=255)
                                                                               atatAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAACATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAg                                                                   >  1:3616151/1‑150 (MQ=255)
                                                                               atatAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAACATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAg                                                                   >  1:1641267/1‑150 (MQ=255)
                                                                                tataACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAACATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGt                                                                  <  2:717676/150‑1 (MQ=255)
                                                                                tataACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAACATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGt                                                                  >  1:4226430/1‑150 (MQ=255)
                                                                                 ataACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAACATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTg                                                                 <  1:2111120/150‑1 (MQ=255)
                                                                                   aaCTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAACATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCa                                                               >  2:1332378/1‑150 (MQ=255)
                                                                                   aaCTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAACATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCa                                                               <  2:400621/150‑1 (MQ=255)
                                                                                     cTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAACATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCaaa                                                             >  1:3811560/1‑150 (MQ=255)
                                                                                     cTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAACATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCaaa                                                             <  1:3336490/150‑1 (MQ=255)
                                                                                        tCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAACATGTCAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCCCCATCACCACCATTTCTGGCGCAACAAGTGCAAACTg                                                          <  2:3677982/150‑1 (MQ=255)
                                                                                         cAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAACATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGc                                                         >  1:1089866/1‑150 (MQ=255)
                                                                                         cAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAACATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGc                                                         <  1:771921/150‑1 (MQ=255)
                                                                                              aaaTATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAACATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTc                                                    <  1:2743268/150‑1 (MQ=255)
                                                                                              aaaTATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAACATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTc                                                    <  2:3005079/150‑1 (MQ=255)
                                                                                               aaTATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAACATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCg                                                   >  1:2427517/1‑150 (MQ=255)
                                                                                                atatCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAACATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGt                                                  <  1:3681822/150‑1 (MQ=255)
                                                                                                atatCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAACATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGt                                                  >  1:2150465/1‑150 (MQ=255)
                                                                                                 tatCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAACATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGtt                                                 <  2:417754/150‑1 (MQ=255)
                                                                                                  atCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAACATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTa                                                >  1:3957674/1‑150 (MQ=255)
                                                                                                      tcatcaAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAACATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCg                                            >  1:2082338/1‑150 (MQ=255)
                                                                                                          caAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAACATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCAt                                        >  1:478574/1‑150 (MQ=255)
                                                                                                          caAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAACATGTTAGTACGTCCGGTGGAGAAGGAGATATGACACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCAt                                        <  2:3500630/150‑1 (MQ=255)
                                                                                                           aaaTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAACATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATa                                       <  1:2663998/150‑1 (MQ=255)
                                                                                                            aaTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAACATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATaa                                      >  2:4318086/1‑150 (MQ=255)
                                                                                                                ggcggcAAACAGTGCCAGATGCGCTGCGCCATTGAACATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGc                                  >  2:3444538/1‑150 (MQ=255)
                                                                                                                    gcAAACAGTGCCAGATGCGCTGCGCCATTGAACATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAAtt                              <  2:471029/150‑1 (MQ=255)
                                                                                                                    gcAAACAGTGCCAGATGCGCTGCGCCATTGAACATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAAtt                              <  2:2893830/150‑1 (MQ=255)
                                                                                                                    gcAAACAGTGCCAGATGCGCTGCGCCATTGAACATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAAtt                              <  1:122550/150‑1 (MQ=255)
                                                                                                                      aaaCAGTGCCAGATGCGCTGCGCCATTGAACATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAg                            >  2:4239933/1‑150 (MQ=255)
                                                                                                                      aaaCAGTGCCAGATGCGCTGCGCCATTGAACATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAg                            >  2:2824391/1‑150 (MQ=255)
                                                                                                                       aaCAGTGCCAGATGCGCTGCGCCATTGAACATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGc                           <  1:1333390/150‑1 (MQ=255)
                                                                                                                        aCAGTGCCAGATGCGCTGCGCCATTGAACATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCg                          <  1:4337493/150‑1 (MQ=255)
                                                                                                                          aGTGCCAGATGCGCTGCGCCATTGAACATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTg                        <  2:2009323/150‑1 (MQ=255)
                                                                                                                               cAGATGCGCTGCGCCATTGAACATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTgacgac                   <  1:728063/150‑1 (MQ=255)
                                                                                                                                 gATGCGCTGCGCCATTGAACATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAg                 <  1:1865347/150‑1 (MQ=255)
                                                                                                                                  aTGCGCTGCGCCATTGAACATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAgg                <  1:676126/150‑1 (MQ=255)
                                                                                                                                   tgcgctgcgcCATTGAACATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGt               <  2:3225211/150‑1 (MQ=255)
                                                                                                                                        tgcgcCATTGAACATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGt          >  1:3049556/1‑150 (MQ=255)
                                                                                                                                          cgcCATTGAACATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTaa        <  2:2095468/150‑1 (MQ=255)
                                                                                                                                           gcCATTGAACATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTAAg       <  2:4099125/150‑1 (MQ=255)
                                                                                                                                           gcCATTGAACATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTAAg       >  2:578987/1‑150 (MQ=255)
                                                                                                                                             cATTGAACATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTAAGCg     >  2:1475579/1‑150 (MQ=255)
                                                                                                                                             cATTGAACATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTAAGCg     >  2:187921/1‑150 (MQ=255)
                                                                                                                                               ttGAACATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTAAGCGaa   >  2:2686567/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAA  >  AP012306/1076089‑1076382

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑