Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP012306202,4170TG23.5% 676.4 / 27.7 278V409G (GTA→GGA) tilStRNA(Ile)‑lysidine synthetase
Reads supporting (aligned to +/- strand):  ref base T (100/112);  new base G (65/0);  total (166/112)
Fisher's exact test for biased strand distribution p-value = 3.80e-18
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 319 total aligned reads displayed.

TTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTG‑CGGCGGCAGGGGTATTTGTGA‑CGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTACCACCACCGTACCG  >  AP012306/202269‑202566
                                                                                                                                                     |                                                                                                                                                      
 tCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAAAGCTGATTG‑CGGCGGCAGGGGGAt                                                                                                                                                      >  2:744101/1‑150 (MQ=255)
  cAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTG‑CGGCGGCAGGGGGAtt                                                                                                                                                     >  1:736838/1‑150 (MQ=255)
   aaaGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTG‑CGGCGGCAGGGGGAttt                                                                                                                                                    >  1:1442312/1‑150 (MQ=255)
      gcgcCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTG‑CGGCGGCAGGGGTATTtgtg                                                                                                                                                 <  1:4352041/150‑1 (MQ=255)
      gcgcCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTG‑CGGCGGCAGGGGTATTtgtg                                                                                                                                                 <  1:1478142/150‑1 (MQ=255)
         ccAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTG‑CGGGGGGAGGGGGGTTTGTGA‑Cg                                                                                                                                             >  2:2449872/1‑150 (MQ=255)
         ccAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTG‑CGGCGGCAGGGGGATTTGTGA‑Cg                                                                                                                                             >  1:2606205/1‑150 (MQ=255)
            ggATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTG‑CGGCGGCAGGGGTATTTGTGA‑CGCaa                                                                                                                                          <  2:3079708/150‑1 (MQ=255)
            ggATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTG‑CGGCGGCAGGGGGATTTTTGg‑cgcca                                                                                                                                          >  2:2581717/1‑148 (MQ=255)
              aTTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTG‑CGGCGGCAGGGGTATTTGTGA‑CGCaaga                                                                                                                                        <  1:970899/150‑1 (MQ=255)
                 gctgcATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATTGCGAAACGCTGATTGCCGCCGGCGGGGGATTTTTGGA‑CGCagaaag                                                                                                                                      >  1:4327476/1‑145 (MQ=255)
                    gcATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGGTTG‑CGGCGGGAGGGGGGTTTTTGACCGCAAGAggggt                                                                                                                                   >  2:910454/1‑148 (MQ=255)
                     cATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTG‑CAGCGGCCGGGGGATTTGTGA‑CGCCAAAAGGtgtg                                                                                                                                 >  1:1015114/1‑150 (MQ=255)
                      atatTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTG‑CGGCGGGAGGGGGATTTTTGG‑CGCaaaaaagggggg                                                                                                                                >  2:2636431/1‑143 (MQ=255)
                          tGTCGGGCGTAACGACGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTG‑CGGCGGCCGGGGGATTTGTGA‑CGCAAGAAGGTGTGGCTGa                                                                                                                            >  2:650368/1‑150 (MQ=255)
                             cGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTG‑CGGCGGCAGGGGGAGTTGTGA‑CGCAAGAAGGTGTGGCTGAAgg                                                                                                                         >  1:3436865/1‑150 (MQ=255)
                                 cGTAACGGCGGACGTAAGCTAAAGGAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTGTATGGCGAAACGCTGATTG‑CGGCGGCAGGGGTATTTGTGA‑CGCAAGAAGGTGTGGCTGAAGGTgag                                                                                                                     <  2:3307503/150‑1 (MQ=255)
                                  gTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTG‑CGGCGGCAGGGGGATTTGTGA‑CGCAAGAAGGTGTGGCTGAAGGTggga                                                                                                                    >  1:1618868/1‑150 (MQ=255)
                                   tAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTG‑CGGCGGCAGGGGGATTTGTGA‑CGCAAGAAGGTGTGGCTGAAGGGGAGaa                                                                                                                   >  1:3257998/1‑150 (MQ=255)
                                      cggcggACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTG‑CGGCGGCAGGGGTATTTGTGA‑CGCAAGAAGGTGTGGCTGAAGGTGAGAATgg                                                                                                                >  1:2746924/1‑150 (MQ=255)
                                        gcggACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTG‑CGGCGGCAGGGGTATTTGTGA‑CGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCg                                                                                                              >  1:2478052/1‑150 (MQ=255)
                                         cggACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTG‑CGGCGGCAGGGGTATTTGTGA‑CGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGt                                                                                                             >  1:3874627/1‑150 (MQ=255)
                                         cggACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTG‑CGGCGGCAGGGGGATTTGTGA‑CGCAAGAAGGTGTGGCTGAAGGGGGGAATGGCgg                                                                                                             >  2:802224/1‑149 (MQ=255)
                                           gACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTG‑CGGCGGCAGGGGTATTTGTGA‑CGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTaa                                                                                                           >  1:678047/1‑150 (MQ=255)
                                           gACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTG‑CGGCGGCAGGGGGATTTGTGA‑CGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTaa                                                                                                           >  2:3942921/1‑150 (MQ=255)
                                              gTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTG‑CGGCGGCAGGGGTATTTGTGA‑CGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGtt                                                                                                        >  1:2346138/1‑150 (MQ=255)
                                              gTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTG‑CGGCGGCAGGGGCCTTTGTGA‑CGCAAAAAGGGGTGGCTGAAGGGGGGAATTGCGGAAggt                                                                                                        >  2:446024/1‑148 (MQ=255)
                                                aaGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTG‑CGGCGGCAGGGGGATTTGTGA‑CGCAAGAAGGTGTGGCTGAAGGGGGGAATGGCGTAAGgttt                                                                                                      >  2:4246502/1‑150 (MQ=255)
                                                 aGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTG‑CGGCGGCAGGGGTATTTGTGA‑CGCAAGAAGGTGTGGCTGGAGGTGAGAATGGTGTAAGttttt                                                                                                     >  1:4246910/1‑149 (MQ=255)
                                                    tAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTG‑CGGCGGCAGGGGGATTTGTGA‑CGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCt                                                                                                  >  2:3263999/1‑150 (MQ=255)
                                                        gAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTG‑CGGCGGCAGGGGGATTTGTGA‑CGCAAGAAGGTGTGGCTGGAGGGGAGAATGGGGTAAGTTTTGTCTGGCa                                                                                              >  2:2799226/1‑150 (MQ=255)
                                                         aaaaTCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTG‑CGGCGGCAGGGGTATTTGTGA‑CGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAg                                                                                             <  2:1981774/150‑1 (MQ=255)
                                                          aaaTCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTG‑CGGCGGCAGGGGTATTTGTGA‑CGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGa                                                                                            <  1:1850106/150‑1 (MQ=255)
                                                          aaaTCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTG‑CGGCGGCAGGGGGATTTGTGA‑CGCTAGAAAGGGTGGGTGAAGGGGAGAAAGGCGTAAAGTTTGTGTGGCAGa                                                                                            >  2:1052432/1‑150 (MQ=255)
                                                          aaaTCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTG‑CGGCGGCAGGGGGATTTGGGA‑CGCAAGAAGGTGTGGCTGAAGGGGAGAATGGCGTAAGTTTTTTCTGGCAGa                                                                                            >  2:1149293/1‑150 (MQ=255)
                                                               tGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTG‑CGGCGGCAGGGGTATTTGTGA‑CGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACg                                                                                       >  1:1701993/1‑150 (MQ=255)
                                                               tGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTG‑CGGCGGCAGGGGTATTTGTGA‑CGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACg                                                                                       >  1:3811456/1‑150 (MQ=255)
                                                                ggCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTG‑CGGCGGCAGGGGTATTTGTGA‑CGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGc                                                                                      <  1:370516/150‑1 (MQ=255)
                                                                  cAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTG‑CGGCGGCAGGGGTATTTGTGA‑CGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCtt                                                                                    <  2:2020872/150‑1 (MQ=255)
                                                                    agagCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTG‑CGGCGGCAGGGGTATTTGTGA‑CGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAg                                                                                  <  1:926368/150‑1 (MQ=255)
                                                                    agagCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTG‑CGGCGGCAGGGGGATTTGTGA‑CGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAg                                                                                  >  2:1428418/1‑150 (MQ=255)
                                                                    agagCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTG‑CGGCGGCAGGGGGATTTGTGA‑CGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAAAGCTTAg                                                                                  >  2:3521974/1‑150 (MQ=255)
                                                                     gagCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTG‑CGGCGGCAGGGGGATTTGTGA‑CGCAAGAAGGTGTGGCTGAAGGGGCGAATGGCGTTAGTTTTGTCTGGCAGGAAAAGCTTAgg                                                                                 >  2:2698911/1‑149 (MQ=255)
                                                                        cTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTG‑CGGCGGCAGGGGTATTTGTGA‑CGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTaa                                                                              >  1:1339364/1‑150 (MQ=255)
                                                                         tGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTG‑CGGCGGCAGGGGTATTTGTGA‑CGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAg                                                                             <  2:851077/150‑1 (MQ=255)
                                                                          gggCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTG‑CGGCGGCAGGGGTATTTGTGA‑CGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGt                                                                            <  2:2161656/150‑1 (MQ=255)
                                                                          gggCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTG‑CGGCGGCAGGGGTATTTGTGA‑CGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGt                                                                            <  1:3408073/150‑1 (MQ=255)
                                                                           ggCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTG‑CGGCGGCAGGGGTATTTGTGA‑CGCAAGAAGGTGTGGCTGAAGGCGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTg                                                                           >  1:1910701/1‑150 (MQ=255)
                                                                              gTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTG‑CGGCGGCAGGGGGATTTGTGA‑CGCAAGAAGGGGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGaaa                                                                        >  1:2641407/1‑150 (MQ=255)
                                                                                gccgccGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTG‑CGGCGGCAGGGGTATTTGTGA‑CGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGc                                                                      >  1:527469/1‑150 (MQ=255)
                                                                                gccgccGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTG‑CGGCGGCAGGGGTATTTGTGA‑CGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGc                                                                      <  1:125095/150‑1 (MQ=255)
                                                                                  cgccgTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTG‑CGGCGGCAGGGGTATTTGTGA‑CGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCg                                                                    <  2:3380569/150‑1 (MQ=255)
                                                                                  cgccgTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTG‑CGGCGGCAGGGGTATTTGTGA‑CGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCg                                                                    <  1:2069531/150‑1 (MQ=255)
                                                                                   gccgTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTG‑CGGCGGCAGGGGGATTTGTGA‑CGCAAGAAGGTTTGGCTGAAAGTGGGAATGGCGGAAGGTTTGTCTGGGAGAAAAAGCTTAGTTAAGGGAAAACCgg                                                                   >  2:236665/1‑150 (MQ=255)
                                                                                   gccgTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTG‑CGGCGGCAGGGGGATTTGTGA‑CGCAAGAAGGTGTGGCTGAAGGGGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTTGTTAAGTGAAAGCCgg                                                                   >  2:942323/1‑150 (MQ=255)
                                                                                     cgTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTG‑CGGCGGCAGGGGTATTTGTGA‑CGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGAt                                                                 <  2:186549/150‑1 (MQ=255)
                                                                                     cgTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTG‑CGGCGGCAGGGGTATTTGTGA‑CGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGAt                                                                 <  2:1911896/150‑1 (MQ=255)
                                                                                     cgTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTG‑CGGCGGCAGGGGTATTTGTGA‑CGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGAt                                                                 <  2:3911444/150‑1 (MQ=255)
                                                                                     cgTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTG‑CGGCGGCAGGGGGATTTTTGA‑CGCAAAAAGGTGTTGCTGAAGGGGAGAATGGCGTTAGTTTTGTCTGGCAGAAAAACCCTGGTTAAAGGAAAACCgggt                                                                 >  2:4307908/1‑148 (MQ=255)
                                                                                      gTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTG‑CGGCGGCAGGGGTATTTGTGA‑CGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATa                                                                >  1:3641194/1‑150 (MQ=255)
                                                                                           tACGTGACGCCACGCCACTGCTGTTTTATGGCGAAACGCTGATTG‑CGGCGGCAGGGGTATTTGTGA‑CGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACg                                                           <  1:3980655/150‑1 (MQ=255)
                                                                                           tACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTG‑CGGCGGCAGGGGTATTTGTGA‑CGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACg                                                           >  1:3131579/1‑150 (MQ=255)
                                                                                            aCGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTG‑CGGCGGCAGGGGTATTTGTGA‑CGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGc                                                          <  1:1947807/150‑1 (MQ=255)
                                                                                               tGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTG‑CGGCGGCAGGGGGATTTGTGA‑CGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATc                                                       >  2:531501/1‑150 (MQ=255)
                                                                                                 acacCACGCCACTGCTGTTTTATGGCGAAACGCTGATTG‑CGGCGGCAGGGGTATTTGTGA‑CGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGGAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCaa                                                     >  2:3165777/1‑150 (MQ=255)
                                                                                                  caccacGCCACTGCTGTTTTATGGCGAAACGCTGATTG‑CGGCGGCAGGGGTATTTGTGA‑CGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCaaa                                                    >  1:239055/1‑150 (MQ=255)
                                                                                                  caccacGCCACTGCTGTTTTATGGCGAAACGCTGATTG‑CGGCGGCAGGGGGATTTGTGA‑CGCAAGAAGGTGTGGCTGAAGGGGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCaaa                                                    >  2:227528/1‑150 (MQ=255)
                                                                                                   accacGCCACTGCTGTTTTATGGCGAAACGCTGATTG‑CGGCGGCAGGGGGATTTGTGA‑CGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAAc                                                   >  1:293603/1‑150 (MQ=255)
                                                                                                         ccACTGCTGTTTTATGGCGAAACGCTGATTG‑CGGCGGCAGGGGTATTTGTGA‑CGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCa                                             <  1:3320171/150‑1 (MQ=255)
                                                                                                           aCTGCTGTTTTATGGCGAAACGCTGATTG‑CGGCGGCAGGGGTATTTGTGA‑CGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATc                                           >  2:3710452/1‑150 (MQ=255)
                                                                                                             tgctgTTTTATGGCGAAACGCTGATTG‑CGGCGGCAGGGGTATTTGTGA‑CGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCg                                         >  1:1378458/1‑150 (MQ=255)
                                                                                                              gctgTTTTATGGCGAAACGCTGATTG‑CGGCGGCAGGGGGATTTGTGA‑CGCAAGGAGGTGTGGCTGTAGGTGTGAATGGCGGAAGTTTTGTCTGGAAGAAAACGCTTTGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCgg                                        >  2:3850832/1‑150 (MQ=255)
                                                                                                                tgTTTTATGGCGAAACGCTGATTG‑CGGCGGCAGGGGTATTTGTGA‑CGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCg                                      <  2:2933807/150‑1 (MQ=255)
                                                                                                                tgTTTTATGGCGAAACGCTGATTG‑CGGCGGCAGGGGGATTTGTGA‑CGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAACCGGATAAGACGCATCAAACGTCGCATCCGGCg                                      >  2:2877291/1‑150 (MQ=255)
                                                                                                                tgTTTTATGGCGAAACGCTGATTG‑CGGCGGCAGGGGGATTTGTGA‑CGCAAGAAGGTGTGGCTGAAGGGGAGAATGGGGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCg                                      >  2:411623/1‑150 (MQ=255)
                                                                                                                  ttttATGGCGAAACGCTGATTG‑CGGCGGCAGGGGTATTTGTGA‑CGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGGTAAGACGCATCAAACGTCGCATCCGGCGaa                                    >  1:1144402/1‑150 (MQ=255)
                                                                                                                  ttttATGGCGAAACGCTGATTG‑CGGCGGCAGGGGTATTTGTGA‑CGCAAGAAGGTGTGGCTGAAGGGGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGaa                                    >  2:2758243/1‑150 (MQ=255)
                                                                                                                  ttttATGGCGAAACGCTGATTG‑CGGCGGCAGGGGCATTTGTGA‑CGCAAGAAGGTGGGGCTGAAGGAGAGAATTGCGGAAGTTTTTTCTGGCAGAAAACGCTTAGTTAAATAAAAACCGGATAAGACGCATCAAACGTCCCATCCGGCGaa                                    >  2:1489267/1‑150 (MQ=255)
                                                                                                                    ttATGGCGAAACGCTGATTG‑CGGCGGCAGGGGTATTTGTGA‑CGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAg                                  <  1:1109128/150‑1 (MQ=255)
                                                                                                                     tATGGCGAAACGCTGATTG‑CGGCGGCAGGGGTATTTGTGA‑CGCAAGAAGGTGTGGCTGAAGGGGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGt                                 >  2:2113949/1‑150 (MQ=255)
                                                                                                                        ggCGAAACGCTGATTG‑CGGCGGCAGGGGTATTTGTGA‑CGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCaa                              >  1:2895587/1‑150 (MQ=255)
                                                                                                                             aaCGCTGATTG‑CGGCGGCAGGGGTATTTGTGA‑CGCAAGAAGGTGTGGCTGAAGGGGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAgg                         >  2:1293830/1‑150 (MQ=255)
                                                                                                                               cGCTGATTG‑CGGCGGCAGGGGTATTTGTGA‑CGCAAGAAGGTGTGGCTGAAGGGGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGAt                       >  2:3623030/1‑150 (MQ=255)
                                                                                                                                 cTGATTG‑CGGCGGCAGGGGTATTTGTGA‑CGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTc                     >  1:782152/1‑150 (MQ=255)
                                                                                                                                  tGATTG‑CGGCGGCAGGGGTATTTGTGA‑CGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCg                    >  2:3759098/1‑150 (MQ=255)
                                                                                                                                    aTTG‑CGGCGGCAGGGGTATTTGTGA‑CGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCt                  >  1:4207375/1‑150 (MQ=255)
                                                                                                                                      tG‑CGGCGGCAGGGGTATTTGTGA‑CGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTa                <  1:2786449/150‑1 (MQ=255)
                                                                                                                                      tG‑CGGCGGCAGGGGTATTTGTGA‑CGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTa                <  1:491292/150‑1 (MQ=255)
                                                                                                                                         cggcggCAGGGGTATTTGTGA‑CGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTacc              >  2:3550508/1‑150 (MQ=255)
                                                                                                                                            cggcAGGGGTATTTGTGA‑CGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTaccacc           >  2:1596401/1‑150 (MQ=255)
                                                                                                                                            cggcAGGGGTATTTGTGA‑CGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTaccacc           >  2:1747730/1‑150 (MQ=255)
                                                                                                                                            cggcAGGGGTATTTGTGA‑CGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTaccacc           <  1:1175492/150‑1 (MQ=255)
                                                                                                                                              gcAGGGGTATTTGTGA‑CGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTaccaccac         <  2:618517/150‑1 (MQ=255)
                                                                                                                                               cAGGGGTATTTGTGA‑CGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTaccaccacc        <  1:2420486/150‑1 (MQ=255)
                                                                                                                                               cAGGGGTATTTGTGA‑CGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTaccaccacc        >  1:4086995/1‑150 (MQ=255)
                                                                                                                                                aGGGGTATTTGTGA‑CGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTACCACCACCg       >  1:1457877/1‑150 (MQ=255)
                                                                                                                                                aGGGGTATTTGTGA‑CGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTACCACCACCg       >  1:394489/1‑150 (MQ=255)
                                                                                                                                                aGGGGTATTTGTGA‑CGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTACCACCACCg       >  2:3634594/1‑150 (MQ=255)
                                                                                                                                                 ggggTATTTGTGA‑CGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTACCACCACCGt      <  2:2537368/150‑1 (MQ=255)
                                                                                                                                                     tATTTGTGA‑CGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTACCACCACCGTACCg  <  1:2857262/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
TTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTG‑CGGCGGCAGGGGTATTTGTGA‑CGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTACCACCACCGTACCG  >  AP012306/202269‑202566

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑