BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AP012306	1,486,241	G→A	intergenic (‑2088/‑492)	thrS ← / → yniD	threonyl‑tRNA synthetase/predicted protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AP012306	1,486,241	0	G	A	100.0%	1517.3 / NA	383	intergenic (‑2088/‑492)	thrS/yniD	threonyl‑tRNA synthetase/predicted protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (193/190); total (193/190)

Only 100 of 387 total aligned reads displayed.

CCAATAGCACAAGAGCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGCCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATTATACATCGCCATGAGCAAGGGTAATGAAGACGTCGTGTTATCTTATATATCGACGCTGGGTGCTTTTGC  >  AP012306/1486094‑1486386

   aTAGCACAAGAGCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCttt                                                                                                                                              <  1:2662241/150‑1 (MQ=255)

    tAGCACAAGAGCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTa                                                                                                                                             <  2:3354865/150‑1 (MQ=255)

     aGCACAAGAGCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTaa                                                                                                                                            <  2:954010/150‑1 (MQ=255)

      gCACAAGAGCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTaaa                                                                                                                                           <  1:1859198/150‑1 (MQ=255)

          aaGAGCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACa                                                                                                                                       >  2:4025352/1‑150 (MQ=255)

          aaGAGCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACa                                                                                                                                       <  1:3320262/150‑1 (MQ=255)

          aaGAGCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACa                                                                                                                                       >  2:3081959/1‑150 (MQ=255)

             agCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGt                                                                                                                                    <  2:1170868/150‑1 (MQ=255)

             agCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGt                                                                                                                                    >  2:3003179/1‑150 (MQ=255)

              gCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGtt                                                                                                                                   <  2:1414000/150‑1 (MQ=255)

                ttttAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGa                                                                                                                                 <  1:2766038/150‑1 (MQ=255)

                ttttAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGa                                                                                                                                 >  2:547159/1‑150 (MQ=255)

                 tttAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAg                                                                                                                                >  2:4329872/1‑150 (MQ=255)

                 tttAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAg                                                                                                                                <  1:138875/150‑1 (MQ=255)

                  ttAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGc                                                                                                                               >  2:1100683/1‑150 (MQ=255)

                   tAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCa                                                                                                                              <  1:1559499/150‑1 (MQ=255)

                       cAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGc                                                                                                                          >  1:2867240/1‑150 (MQ=255)

                         aaaaaCTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTa                                                                                                                        <  1:1034379/150‑1 (MQ=255)

                          aaaaCTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTaa                                                                                                                       <  2:2651229/150‑1 (MQ=255)

                            aaCTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAAca                                                                                                                     <  2:1616013/150‑1 (MQ=255)

                            aaCTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATAGCATCACCTTTAAATACAAGTTGAGCAAAGCTAAca                                                                                                                     <  1:3304032/150‑1 (MQ=255)

                             aCTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACATGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAAcat                                                                                                                    >  1:1998391/1‑150 (MQ=255)

                              cTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAAcatc                                                                                                                   <  1:2630534/150‑1 (MQ=255)

                               tGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCGCACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAAcatca                                                                                                                  <  1:3635887/150‑1 (MQ=255)

                               tGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAAcatca                                                                                                                  <  1:4008434/150‑1 (MQ=255)

                               tGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAAcatca                                                                                                                  >  2:1323539/1‑150 (MQ=255)

                               tGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAAcatca                                                                                                                  >  2:3610997/1‑150 (MQ=255)

                                ggTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAAcatcat                                                                                                                 <  1:3188174/150‑1 (MQ=255)

                                ggTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAAcatcat                                                                                                                 <  2:1488637/150‑1 (MQ=255)

                                ggTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAAcatcat                                                                                                                 >  2:3498616/1‑150 (MQ=255)

                                ggTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAAcatcat                                                                                                                 <  1:1619894/150‑1 (MQ=255)

                                  tgatgaGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATgg                                                                                                               >  1:3588617/1‑150 (MQ=255)

                                  tgatgaGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATgg                                                                                                               <  1:3779876/150‑1 (MQ=255)

                                  tgatgaGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATgg                                                                                                               >  1:797346/1‑150 (MQ=255)

                                  tgatgaGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATgg                                                                                                               <  2:785963/150‑1 (MQ=255)

                                    atgaGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGAt                                                                                                             >  1:294803/1‑150 (MQ=255)

                                    atgaGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGAt                                                                                                             >  2:911153/1‑150 (MQ=255)

                                      gaGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGAttt                                                                                                           >  2:3180907/1‑150 (MQ=255)

                                      gaGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGAttt                                                                                                           <  2:2272907/150‑1 (MQ=255)

                                      gaGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAAT‑ATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGGGCAAAGCTAACATCATGGATtta                                                                                                          >  1:2577395/1‑150 (MQ=255)

                                       aGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATtta                                                                                                          >  1:2261839/1‑150 (MQ=255)

                                          aTTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATttatta                                                                                                       <  2:842185/150‑1 (MQ=255)

                                           ttttAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTaa                                                                                                      >  2:1687083/1‑150 (MQ=255)

                                            tttAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTaaa                                                                                                     >  2:2971120/1‑150 (MQ=255)

                                            tttAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTaaa                                                                                                     <  2:1000557/150‑1 (MQ=255)

                                              tAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAg                                                                                                   <  1:2287399/150‑1 (MQ=255)

                                                 ccATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAg                                                                                                <  1:892599/150‑1 (MQ=255)

                                                   aTGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCt                                                                                              <  2:73962/150‑1 (MQ=255)

                                                   aTGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCt                                                                                              <  1:20911/150‑1 (MQ=255)

                                                    tGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTa                                                                                             <  1:1758044/150‑1 (MQ=255)

                                                      aaaaTACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACa                                                                                           <  2:1359360/150‑1 (MQ=255)

                                                      aaaaTACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACa                                                                                           <  1:1728819/150‑1 (MQ=255)

                                                       aaaTACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAg                                                                                          <  1:2415051/150‑1 (MQ=255)

                                                       aaaTACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAg                                                                                          >  2:2144352/1‑150 (MQ=255)

                                                          tACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGcac                                                                                       >  1:555763/1‑150 (MQ=255)

                                                          tACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGcac                                                                                       <  1:122839/150‑1 (MQ=255)

                                                             atgatgGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAgg                                                                                    >  1:2244301/1‑150 (MQ=255)

                                                             atgatgGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAgg                                                                                    <  2:1740989/150‑1 (MQ=255)

                                                              tgatgGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAggg                                                                                   >  1:3919840/1‑150 (MQ=255)

                                                               gatgGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGa                                                                                  >  1:93210/1‑150 (MQ=255)

                                                               gatgGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGa                                                                                  >  1:4281576/1‑150 (MQ=255)

                                                               gatgGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGa                                                                                  >  1:505283/1‑150 (MQ=255)

                                                                   gTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAAccc                                                                              <  2:493405/150‑1 (MQ=255)

                                                                    tGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAAcccc                                                                             <  1:483757/150‑1 (MQ=255)

                                                                     gAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCt                                                                            <  1:744269/150‑1 (MQ=255)

                                                                     gAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCt                                                                            <  1:616987/150‑1 (MQ=255)

                                                                       gCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGc                                                                          <  1:179151/150‑1 (MQ=255)

                                                                         cAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCAt                                                                        <  2:3695704/150‑1 (MQ=255)

                                                                            gATTATACGCCGCAATGGAAAATAATCACCCTTTGAGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATtat                                                                     <  2:2300663/150‑1 (MQ=255)

                                                                              ttATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATTATAc                                                                   <  1:2230928/150‑1 (MQ=255)

                                                                               tataCGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATTATACa                                                                  >  1:2694670/1‑150 (MQ=255)

                                                                                 taCGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATTATACATc                                                                <  2:3756497/150‑1 (MQ=255)

                                                                                 taCGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATTATACATc                                                                <  1:4055190/150‑1 (MQ=255)

                                                                                      cgcAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATTATACATCGCCAt                                                           >  2:3787531/1‑150 (MQ=255)

                                                                                       gcAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATTATACATCGCCATg                                                          >  1:343438/1‑150 (MQ=255)

                                                                                                   aatCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATTATACATCGCCATGAGCAAGGGTAAt                                              >  1:730885/1‑150 (MQ=255)

                                                                                                   aatCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATTATACATCGCCATGAGCAAGGGTAAt                                              <  1:4155925/150‑1 (MQ=255)

                                                                                                   aatCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATTATACATCGCCATGAGCAAGGGTAAt                                              <  1:3715144/150‑1 (MQ=255)

                                                                                                    atCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATTATACATCGCCATGAGCAAGGGTAATg                                             <  2:662538/150‑1 (MQ=255)

                                                                                                        cccTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATTATACATCGCCATGAGCAAGGGTAATGAAGa                                         <  2:2840992/150‑1 (MQ=255)

                                                                                                         ccTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATTATACATCGCCATGAGCAAGGGTAATGAAGAc                                        <  1:4331961/150‑1 (MQ=255)

                                                                                                          cTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATTATACATCGCCATGAGCAAGGGTAATGAAGAcg                                       <  1:1081713/150‑1 (MQ=255)

                                                                                                                gtgtCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATTATACATCGCCATGAGCAAGGGTAATGAAGACGTCgtgt                                 <  2:2757777/150‑1 (MQ=255)

                                                                                                                gtgtCACACGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATTATACATCGCCATGAGCAAGGGTAATGAAGACGTCgtgt                                 <  2:4304234/150‑1 (MQ=255)

                                                                                                                     acacGGTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATTATACATCGCCATGAGCAAGGGTAATGAAGACGTCGTGTTATCt                            <  1:4373083/150‑1 (MQ=255)

                                                                                                                          gTTCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATTATACATCGCCATGAGCAAGGGTAATGAAGACGTCGTGTTATCTtatat                       <  2:33796/150‑1 (MQ=255)

                                                                                                                           ttCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATTATACATCGCCATGAGCAAGGGTAATGAAGACGTCGTGTTATCTtatata                      <  2:4107669/150‑1 (MQ=255)

                                                                                                                            tCCTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATTATACATCGCCATGAGCAAGGGTAATGAAGACGTCGTGTTATCTTatatat                     >  2:2911178/1‑150 (MQ=255)

                                                                                                                             ccTCTCTAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATTATACATCGCCATGAGCAAGGGTAATGAAGACGTCGTGTTATCTTATATATc                    >  1:191875/1‑150 (MQ=255)

                                                                                                                              ctctctAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATTATACATCGCCATGAGCAAGGGTAATGAAGACGTCGTGTTATCTTATATATCg                   <  2:1209035/150‑1 (MQ=255)

                                                                                                                              ctctctAAAATTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATTATACATCGCCATGAGCAAGGGTAATGAAGACGTCGTGTTATCTTATATATCg                   >  1:2178886/1‑150 (MQ=255)

                                                                                                                                      aaTTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATTATACATCGCCATGAGCAAGGGTAATGAAGACGTCGTGTTATCTTATATATCGACGCTggg           >  1:662118/1‑150 (MQ=255)

                                                                                                                                       aTTAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATTATACATCGCCATGAGCAAGGGTAATGAAGACGTCGTGTTATCTTATATATCGACGCTGGGt          >  2:2001278/1‑150 (MQ=255)

                                                                                                                                        ttAATGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATTATACATCGCCATGAGCAAGGGTAATGAAGACGTCGTGTTATCTTATATATCGACGCTGGGTg         >  2:1530462/1‑150 (MQ=255)

                                                                                                                                          aaTGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATTATACATCGCCATGAGCAAGGGTAATGAAGACGTCGTGTTATCTTATATATCGACGCTGGGTGCt       >  1:2349564/1‑150 (MQ=255)

                                                                                                                                           aTGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATTATACATCGCCATGAGCAAGGGTAATGAAGACGTCGTGTTATCTTATATATCGACGCTGGGTGCtt      >  1:1479325/1‑150 (MQ=255)

                                                                                                                                           aTGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATTATACATCGCCATGAGCAAGGGTAATGAAGACGTCGTGTTATCTTATATATCGACGCTGGGTGCtt      <  1:1167639/150‑1 (MQ=255)

                                                                                                                                            tGGCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATTATACATCGCCATGAGCAAGGGTAATGAAGACGTCGTGTTATCTTATATATCGACGCTGGGTGCttt     >  2:2394921/1‑150 (MQ=255)

                                                                                                                                              gCATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATTATACATCGCCATGAGCAAGGGTAATGAAGACGTCGTGTTATCTTATATATCGACGCTGGGTGCTTTTg   <  2:1891484/150‑1 (MQ=255)

                                                                                                                                               cATCACCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATTATACATCGCCATGAGCAAGGGTAATGAAGACGTCGTGTTATCTTATATATCGACGCTGGGTGCTTTTGc  >  2:3952553/1‑150 (MQ=255)

CCAATAGCACAAGAGCTTTTAAACAAAAAACTGGTGATGAGTATTTTAGCCATGAAAATACATGATGGTGAGCCAGGATTATACGCCGCAATGGAAAATAATCACCCTTTGTGTGTCACACGGTTCCTCTCTAAAATTAATGGCATCGCCTTTAAATACAAGTTGAGCAAAGCTAACATCATGGATTTATTAAAAGGAGCTACAGCACAGGGAACCCCTGCATTATACATCGCCATGAGCAAGGGTAATGAAGACGTCGTGTTATCTTATATATCGACGCTGGGTGCTTTTGC  >  AP012306/1486094‑1486386

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`