Predicted mutation
evidence seq id position mutation annotation gene description
RA AP012306 2,073,848 Δ2 bp intergenic (+106/+161) lysV → / ← xapR tRNA‑Lys/DNA‑binding transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123062,073,8470G.100.0% 496.0 / NA 110intergenic (+105/+163)lysV/xapRtRNA‑Lys/DNA‑binding transcriptional activator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (26/84);  total (26/84)
*AP0123062,073,8480T.100.0% 496.0 / NA 110intergenic (+106/+162)lysV/xapRtRNA‑Lys/DNA‑binding transcriptional activator
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (26/84);  total (26/84)

Only 100 of 119 total aligned reads displayed.

TTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGTGCTGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGACTACTATTT  >  AP012306/2073699‑2073987
                                                                                                                                                    ||                                                                                                                                           
ttttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCgc                                                                                                                                             >  1:510224/1‑149 (MQ=14)
 tttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCgcg                                                                                                                                            >  2:2869728/1‑148 (MQ=11)
 tttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCgcg                                                                                                                                            >  2:3692675/1‑148 (MQ=11)
  ttAATCAGTTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCgcgg                                                                                                                                           >  1:3600738/1‑147 (MQ=1)
  ttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCgcgg                                                                                                                                           <  1:3416632/150‑4 (MQ=1)
  ttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCgcgg                                                                                                                                           >  1:2210607/1‑147 (MQ=1)
                                                                                                   gAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGa                                       <  1:2686777/150‑1 (MQ=0)
                                                                                                   gAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGa                                       >  1:1925418/1‑150 (MQ=0)
                                                                                                    aaCCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAGAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGaa                                      >  2:1484403/1‑150 (MQ=11)
                                                                                                    aaCCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGaa                                      >  2:3448583/1‑150 (MQ=2)
                                                                                                    aaCCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGaa                                      >  2:717256/1‑150 (MQ=2)
                                                                                                    aaCCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGaa                                      <  2:2032473/150‑1 (MQ=2)
                                                                                                    aaCCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGaa                                      <  2:1862332/150‑1 (MQ=2)
                                                                                                     aCCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGaaa                                     >  2:4078096/1‑150 (MQ=255)
                                                                                                      ccTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAAt                                    >  1:723791/1‑150 (MQ=255)
                                                                                                      ccTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAAt                                    <  1:2171788/150‑1 (MQ=255)
                                                                                                      ccTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAAt                                    <  2:161660/150‑1 (MQ=255)
                                                                                                      ccTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAAt                                    <  2:2203193/150‑1 (MQ=255)
                                                                                                       cTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAAtt                                   <  1:3206697/150‑1 (MQ=255)
                                                                                                       cTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAAtt                                   <  2:2855287/150‑1 (MQ=255)
                                                                                                       cTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAAtt                                   <  2:3681159/150‑1 (MQ=255)
                                                                                                        tGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTg                                  <  2:1773516/150‑1 (MQ=255)
                                                                                                        tGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTg                                  <  2:311363/150‑1 (MQ=255)
                                                                                                        tGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTg                                  <  1:356495/150‑1 (MQ=255)
                                                                                                        tGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTg                                  <  1:1392226/150‑1 (MQ=255)
                                                                                                         gcagcaGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGc                                 <  1:2891594/150‑1 (MQ=255)
                                                                                                         gcagcaGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGc                                 <  2:3194257/150‑1 (MQ=255)
                                                                                                          cagcagGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGcc                                <  2:612764/150‑1 (MQ=255)
                                                                                                           agcagGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAGGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCt                               >  1:2594377/1‑150 (MQ=11)
                                                                                                           agcagGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCt                               <  2:868181/150‑1 (MQ=255)
                                                                                                           agcagGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCt                               >  2:3492690/1‑150 (MQ=255)
                                                                                                            gcagGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTg                              <  1:334926/150‑1 (MQ=255)
                                                                                                            gcagGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTg                              <  1:4016131/150‑1 (MQ=255)
                                                                                                             cagGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGa                             >  1:655380/1‑150 (MQ=255)
                                                                                                              agGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGAt                            >  1:1785154/1‑150 (MQ=255)
                                                                                                              agGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGAt                            <  2:1817841/150‑1 (MQ=255)
                                                                                                               ggTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATg                           >  2:4080411/1‑150 (MQ=255)
                                                                                                               ggTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATg                           <  1:1615038/150‑1 (MQ=255)
                                                                                                               ggTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATg                           <  1:1708917/150‑1 (MQ=255)
                                                                                                               ggTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATg                           <  1:1858520/150‑1 (MQ=255)
                                                                                                               ggTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATg                           <  2:3292796/150‑1 (MQ=255)
                                                                                                               ggTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATg                           <  1:2537634/150‑1 (MQ=255)
                                                                                                               ggTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATg                           >  1:996527/1‑150 (MQ=255)
                                                                                                                gTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATgc                          >  1:1866524/1‑150 (MQ=255)
                                                                                                                gTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATgc                          <  2:2469557/150‑1 (MQ=255)
                                                                                                                 tttGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATgcg                         <  2:2472649/150‑1 (MQ=255)
                                                                                                                 tttGAGTTGAGCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATgcg                         >  2:1118929/1‑150 (MQ=255)
                                                                                                                  ttgagttgagCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATgcgc                        <  2:2803028/150‑1 (MQ=255)
                                                                                                                  ttgagttgagCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATgcgc                        <  2:3894049/150‑1 (MQ=255)
                                                                                                                   tgagttgagCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCt                       >  1:285088/1‑150 (MQ=255)
                                                                                                                    gagttgagCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCtt                      <  1:2930193/150‑1 (MQ=255)
                                                                                                                    gagttgagCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCtt                      <  2:4015458/150‑1 (MQ=255)
                                                                                                                     agttgagCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTc                     >  1:2877954/1‑150 (MQ=255)
                                                                                                                     agttgagCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTc                     <  2:2111225/150‑1 (MQ=255)
                                                                                                                       ttgagCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCAc                   <  2:3910728/150‑1 (MQ=255)
                                                                                                                       ttgagCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCAc                   <  2:2205789/150‑1 (MQ=255)
                                                                                                                        tgagCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACt                  <  1:3402082/150‑1 (MQ=255)
                                                                                                                         gagCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACtt                 >  1:2597077/1‑150 (MQ=255)
                                                                                                                          agCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTa                <  2:4186325/150‑1 (MQ=255)
                                                                                                                          agCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTa                <  1:1487242/150‑1 (MQ=255)
                                                                                                                          agCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTa                <  2:2193547/150‑1 (MQ=255)
                                                                                                                          agCGCAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTAACTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTa                <  1:3797724/150‑1 (MQ=14)
                                                                                                                           gcgcAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAg               <  2:4073019/150‑1 (MQ=255)
                                                                                                                           gcgcAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAg               <  1:3723810/150‑1 (MQ=255)
                                                                                                                           gcgcAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAg               <  2:2519526/150‑1 (MQ=255)
                                                                                                                            cgcAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGc              <  1:1122917/150‑1 (MQ=255)
                                                                                                                            cgcAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGc              <  1:2124945/150‑1 (MQ=255)
                                                                                                                            cgcAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGc              <  1:3940340/150‑1 (MQ=255)
                                                                                                                            cgcAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGc              >  2:3783700/1‑150 (MQ=255)
                                                                                                                            cgcAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGc              >  2:3910744/1‑150 (MQ=255)
                                                                                                                             gcAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCa             <  1:2306929/150‑1 (MQ=255)
                                                                                                                             gcAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCa             <  2:3498313/150‑1 (MQ=255)
                                                                                                                             gcAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCa             <  2:3314521/150‑1 (MQ=255)
                                                                                                                              cAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAg            <  1:2143188/150‑1 (MQ=255)
                                                                                                                              cAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAg            >  1:30318/1‑150 (MQ=255)
                                                                                                                              cAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAg            <  2:2218442/150‑1 (MQ=255)
                                                                                                                              cAGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAg            <  2:4215957/150‑1 (MQ=255)
                                                                                                                               aGCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGa           <  2:1338663/150‑1 (MQ=255)
                                                                                                                                gCGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGac          >  1:2473015/1‑150 (MQ=255)
                                                                                                                                 cGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGact         <  1:3899686/150‑1 (MQ=255)
                                                                                                                                 cGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGact         <  2:2687110/150‑1 (MQ=255)
                                                                                                                                 cGAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGact         <  2:2970677/150‑1 (MQ=255)
                                                                                                                                  gAAACAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGacta        <  1:1458224/150‑1 (MQ=255)
                                                                                                                                   aaaCAACGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGactac       <  1:1522006/150‑1 (MQ=255)
                                                                                                                                    aacaacGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGactact      <  2:2897854/150‑1 (MQ=255)
                                                                                                                                    aacaacGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGactact      <  2:1711888/150‑1 (MQ=255)
                                                                                                                                     acaacGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGActacta     <  2:656484/150‑1 (MQ=255)
                                                                                                                                      caacGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGACTACTAt    <  2:2116850/150‑1 (MQ=255)
                                                                                                                                      caacGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGACTACTAt    <  1:570528/150‑1 (MQ=255)
                                                                                                                                       aacGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGACTACTAtt   <  2:1430221/150‑1 (MQ=255)
                                                                                                                                       aacGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGACTACTAtt   <  1:3092860/150‑1 (MQ=255)
                                                                                                                                       aacGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGACTACTAtt   >  1:2439379/1‑150 (MQ=255)
                                                                                                                                       aacGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGACTACTAtt   <  1:4362883/150‑1 (MQ=255)
                                                                                                                                       aacGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGACTACTAtt   <  2:3340143/150‑1 (MQ=255)
                                                                                                                                       aacGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGACTACTAtt   <  1:4263626/150‑1 (MQ=255)
                                                                                                                                       aacGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCCGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGACTACTAtt   <  1:3996334/150‑1 (MQ=18)
                                                                                                                                        acGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGACTACTAttt  <  2:3706939/150‑1 (MQ=255)
                                                                                                                                        acGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGACTACTAttt  >  2:259794/1‑150 (MQ=255)
                                                                                                                                        acGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGACTACTAttt  <  2:759505/150‑1 (MQ=255)
                                                                                                                                        acGGAGCCGCTC‑‑GC‑GGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGACTACTAttt  <  1:924477/150‑1 (MQ=255)
                                                                                                                                                    ||                                                                                                                                           
TTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGTGCTGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTTGCGAAATTGCCTGATGCGCTTCACTTAGCAGACTACTATTT  >  AP012306/2073699‑2073987

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑