Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123061,393,3790AC24.0% 742.5 / 44.9 275T92P (ACC→CCC) nthDNA glycosylase and apyrimidinic (AP) lyase
Reads supporting (aligned to +/- strand):  ref base A (185/24);  new base C (0/66);  total (185/90)
Fisher's exact test for biased strand distribution p-value = 1.19e-43
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 310 total aligned reads displayed.

CGACCGATGTCAGTGTTAATAAGGCGACGGCGAAACTCTACCCGGTGGCGAATACGCCTGCAGCGATGCTTGAACTGGGCGTTGAAGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAACGTCGTATTAAACACTGCATTCGGCTGGCCGACTATTGCTGTCGACACGCACAT  >  AP012306/1393230‑1393527
                                                                                                                                                     |                                                                                                                                                    
cGACCGATGTCAGTGTTAATAAGGCGACGGCGAAACTCTACCCGGTGGCGAATACGCCTGCAGCGATGCTTGAACTGGGCGTTGAAGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCaaaa                                                                                                                                                      <  1:3668028/150‑1 (MQ=255)
 gACCGATGTCAGTGTTAATAAGGCGACGGCGAAACTCTACCCGGTGGCGAATACGCCTGCAGCGATGCTTGAACTGGGCGTTGAAGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAAc                                                                                                                                                     >  2:3046273/1‑150 (MQ=255)
 gACCGATGTCAGTGTTAATAAGGCGACGGCGAAACTCTACCCGGTGGCGAATACGCCTGCAGCGATGCTTGAACTGGGCGTTGAAGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAAc                                                                                                                                                     >  2:2733490/1‑150 (MQ=255)
 gACCGATGTCAGTGTTAATAAGGCAACGGCGAAACTCTACCCGGTGGCGAATACGCCTGCAGCGATGCTTGAACTGGGCGTTGAAGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAAc                                                                                                                                                     >  2:4216446/1‑150 (MQ=255)
  aCCGATGTCAGTGTTAATAAGGCGACGGCGAAACTCTACCCGGTGGCGAATACGCCTGCAGCGATGCTTGAACTGGGCGTTGAAGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAAcc                                                                                                                                                    >  1:1147279/1‑150 (MQ=255)
    cGATGTCAGTGTTAATAAGGCGACGGCGAAACTCTACCCGGTGGCGAATACGCCTGCAGCGATGCTTGAACTGGGCGTTGAAGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTg                                                                                                                                                  <  1:2013855/150‑1 (MQ=255)
     gATGTCAGTGTTAATAAGGCGACGGCGAAACTCTACCCGGTGGCGAATACGCCTGCAGCGATGCTTGAACTGGGCGTTGAAGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGc                                                                                                                                                 >  1:3120191/1‑150 (MQ=255)
      aTGTCAGTGTTAATAAGGCGACGGCGAAACTCTACCCGGTGGCGAATACGCCTGCAGCGATGCTTGAACTGGGCGTTGAAGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGcc                                                                                                                                                >  1:1780122/1‑150 (MQ=255)
      aTGTCAGTGTTAATAAGGCGACGGCGAAACTCTACCCGGTGGCGAATACGCCTGCAGCGATGCTTGAACTGGGCGTTGAAGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGcc                                                                                                                                                >  2:418416/1‑150 (MQ=255)
       tGTCAGTGTTAATAAGGCGACGGCGAACCTCTACCCGGTGGCGAATACGCCTACAGCGATGCTTGAACTGGGCGTTGAAGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCg                                                                                                                                               <  2:2906700/150‑1 (MQ=255)
       tGTCAGTGTTAATAAGGCGACGGCGAAACTCTACCCGGTGGCGAATACGCCTGCAGCGATGCTTGAACTGGGCGTTGAAGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCg                                                                                                                                               <  1:3585515/150‑1 (MQ=255)
       tGTCAGTGTTAATAAGGCGAAGGCGAAACTCTACCCGGTGGCGAATACGCCTGCAGCGATGCTTGAACTGGGCGTTGAAGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCg                                                                                                                                               >  2:4300840/1‑150 (MQ=255)
        gTCAGTGTTAATAAGGCGACGGCGAAACTCTACCCGGTGGCGAATACGCCTGCAGCGATGCTTGAACTGGGCGTTGAAGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGt                                                                                                                                              >  2:494430/1‑150 (MQ=255)
         tCAGTGTAAATAAGGCGGCGGCGAAACTCTACCGGGGGGGAAATCCCCCTGCAGCGATGCTTGAACGGGGCGTTGAGGGGGTAAAAACCTATACAAAAACGATTGGTTTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTa                                                                                                                                             <  2:1653119/150‑1 (MQ=255)
          cAGTGTTAATAAGGCGACGGCGAAACTCTACCCGGTGGCGAATACGCCTGCAGCGATGCTTGAACTGGGCGTTGAAGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTAt                                                                                                                                            >  1:8423/1‑150 (MQ=255)
          cAGTGTTAATAAGGCGACGGCGAAACTCTACCCGGTGGCGAATACGCCTGCAGCGATGCTTGAACTGGGCGTTGAAGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTAt                                                                                                                                            >  1:3328495/1‑150 (MQ=255)
           aGTGTAAAAAAGGCGAGGGCAAAACTCTCCCGGGGGGCGAATACGCCTGCAGCGATGCTTGAACGGGGCGTTGAAGGGGTGAAAACCTATATAAAAACGTTGGGGTTTTATAACAGAAAAGCAAAAAATATCATAAAACCCTGCCGTATc                                                                                                                                           <  2:3237784/150‑1 (MQ=255)
               ttAATAAGGCGACGGCGAAACTCTACCCGGTGGCGAATACGCCTGCAGCGATGCTTGAACTGGGCGTTGAAGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGc                                                                                                                                       >  2:1508029/1‑150 (MQ=255)
                     aGGCGACGGCGAAACTCTACCCGGTGGCGAATACGCCTGCAGCGATGCTTGAACTGGGCGTTGAAGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGagc                                                                                                                                 >  1:2581538/1‑150 (MQ=255)
                        cGACGGCGAAACTCTACCCGGTGGCGAATACGCCTGCAGCGATGCTTGAACTGGGCGTTGAAGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGagcagc                                                                                                                              >  2:3839339/1‑150 (MQ=255)
                         gACGGCGAAACTCTACCCGGTGGCGAATACGCCTGCAGCGATGCTTGAACTGGGCGTTGAAGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAgcagca                                                                                                                             >  1:3424040/1‑150 (MQ=255)
                           cGGCGAACCTCTACCCGGTGGCGAATACGCCTGCAGCGATGCTTGAACTGGGCGTTGAGGGGGTGAAACCCTATATCAAAACGATTGGGCTTTATAACAGCAAGGCAAAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATa                                                                                                                           <  1:2909759/150‑1 (MQ=255)
                           cGGCGAAACTCTCCCCGGTGGCAAATACGCCTGCAGCGATGCTTGAACTGGGCGTTGAAGGGGTAAAAACCTATATCAAAACGATTGGGCTTTATAACAGAAAAGAAAAAAATATCATAAAACCCTGCCGTATCTTGCTGGAGCAGCATa                                                                                                                           <  2:3401377/150‑1 (MQ=255)
                           cGGCGAAACTCTACCCGGTGGCGAATACGCCTGCAGCGATGCTTGAACTGGGCGTTGAAGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATa                                                                                                                           >  2:2371287/1‑150 (MQ=255)
                                 aaCTCTACCCGGTGGCGAATACGCCTGCAGCGATGCTTGAACTGGGCGTTGAAGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCg                                                                                                                     >  1:3975450/1‑150 (MQ=255)
                                   ctctACCCGGTGGCGAATACGCCTGCAGCGATGCTTGAACTGGGCGTTGAAGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAg                                                                                                                   >  2:2067075/1‑150 (MQ=255)
                                      tACCCGGTGGCGAATACGCCTGCAGCGATGCTTGAACTGGGCGTTGAAGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGtt                                                                                                                >  2:4388606/1‑150 (MQ=255)
                                         cggggggCAAATACGCCTGCAGCGATGCTTGAACGGGGCGTTGAGGGGGTAAAAACTTATATAAAAAGGATTGGTTTTTAAAACAGAAAAGCAAAAAATATCATAAAACCCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCg                                                                                                             <  2:1036371/145‑1 (MQ=255)
                                              ggCGAATACGCCTGCAGCGATGCTTGAACTGGGCGTTGAAGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGa                                                                                                        >  2:648612/1‑150 (MQ=255)
                                                cGAATACGCCTGCAGCGATGCTTGAACTGGGCGTTGAGGGGATGAAACCCTATATCAAAACGATTGGTTTTTATAACAGAAAAGCAAAAAATATCACCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATc                                                                                                      <  2:391705/150‑1 (MQ=255)
                                                 gAATACGCCTGCAGCGATGCTTGAACTGGGCGTTGAAGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCg                                                                                                     >  1:3893682/1‑150 (MQ=255)
                                                 gAATACGCCTGCAGCGATGCTTGAACTGGGCGTTGAAGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATAAAACCCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCg                                                                                                     <  1:4306840/150‑1 (MQ=255)
                                                     aCGCCTGCAGCGATGCTTGAACTGGGCGTTGAAGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGtgct                                                                                                 >  2:3655292/1‑150 (MQ=255)
                                                       gCCTGCAGCGATGCTTGAACTGGGCGTTGAAGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGtgctgc                                                                                               >  1:672411/1‑150 (MQ=255)
                                                             agggATGTTTGAACGGGGCGTTGAAGGGGTAAAACCCTATATCAAAACGTTTGGGTTTTATAACAGAAAAGCAAAAAATATCATCAAACCCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGa                                                                                         <  2:2837603/147‑1 (MQ=255)
                                                               cgttGCTTGAACTGGGGGTGGAAGGGGTAAAAACCTATATCAAAACGATTGGGTTTTATAACAGAAAAGCAAAAAATATCATAAAACCCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAg                                                                                       <  2:2782156/147‑1 (MQ=255)
                                                                   gCTTGAACTGGGCGTTGAAGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCt                                                                                   >  1:4029220/1‑150 (MQ=255)
                                                                    cTTGAACTGGGCGTTGAGGGGGTAAAACCCTATATAAAAACGATTGGGTTTTATAACAGCAAAGAAAAAAATATCATCAAACCCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTg                                                                                  <  2:3123405/150‑1 (MQ=255)
                                                                             ggCGTTGAAGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTa                                                                         >  1:2852834/1‑150 (MQ=255)
                                                                              gCGTTGAGGGGGTAAAAACCTATATAAAAACGTTTGGGTTTTATAACAGAAAAGCAAAAAATATCATCAAACCCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAg                                                                        <  2:173188/150‑1 (MQ=255)
                                                                              gCGTTGAAGGGGGAAAAACCTATATAAAAACGTTTGGGTTTTATAAAAGAAAAGAAGAAAATATCATCAAACCCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAg                                                                        <  2:1181522/150‑1 (MQ=255)
                                                                                gTTGAAGGGTTAAAAACCAATAAAAAACCGTTTGGCTTTAAGAACAGAAGAAAAGAAAATATCAAAAACACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGt                                                                      <  2:2134640/150‑1 (MQ=255)
                                                                                gTTGAAGGGGTGAAAACCTATATAAAAACGATTGGGTTTTATAACAGAAAAGCAAAAAATACCACCAAACCCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGt                                                                      <  2:2652478/150‑1 (MQ=255)
                                                                                 tggAAGGGGTAAAACCCTATATAAAAACGATGGGGCTTTAAAACAGCAAAGCAGAAAATATCACAAAACCCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTc                                                                     <  2:4057701/148‑1 (MQ=255)
                                                                                       gggTAAAACCCTATAACAAAACGATTGGGTTTTATAACAGAAAAGCAAAAAATATCATCAAACCCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTaaaa                                                               <  2:1350095/150‑1 (MQ=255)
                                                                                          taaaacCCTATATAAAACCGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAACCCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAg                                                            <  1:710077/148‑1 (MQ=255)
                                                                                          taaaaaCCTATATCAAAACGATTGGTCTTTATAACAGCAAAGCAGAAAATATCATCAAACCCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAg                                                            <  1:1528061/148‑1 (MQ=255)
                                                                                          taaaaaCCTATAACAAAACGATTGGGCTTTATAACAGCAAAGCAAAAAATATCATCAAACCCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAg                                                            <  2:2887142/148‑1 (MQ=255)
                                                                                          tGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAg                                                            >  1:1833661/1‑150 (MQ=255)
                                                                                          tGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAg                                                            >  1:1309093/1‑150 (MQ=255)
                                                                                           gAAAACCTATAACAAAACGTTGGGGCTTTAAAACAGAAAAGCAAAAAATATCATCAAACCCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGc                                                           <  1:626533/150‑1 (MQ=255)
                                                                                             aaccttatataAAAACGATTGGGTTTTATAACAGAAAAGAAAAAAATACCACCAAACCCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCa                                                         <  2:3997601/145‑1 (MQ=255)
                                                                                             aaaCCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCGCTGCCCGGCGTAGGTCGTAAAACAGCCa                                                         >  1:1031195/1‑150 (MQ=255)
                                                                                              aaCCTATATCAAAACGATTGGGCTTTAAAACAGCAAAGCAAAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCaa                                                        <  1:1203931/150‑1 (MQ=255)
                                                                                               cccTATATCAAAACGATTGGGCTTTATAACAGAAAAGCAAAAAATATCATCAAACCCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAAc                                                       <  2:2278162/149‑1 (MQ=255)
                                                                                                ccTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAAcg                                                      >  1:1463536/1‑150 (MQ=255)
                                                                                                 ttaaataaaaaCGATGGGGTTTTATAACAGAAAAGCAAAAAATATCATAAAACCCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAAcgt                                                     <  1:3197134/143‑1 (MQ=255)
                                                                                                 cTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAAcgt                                                     >  1:2294394/1‑150 (MQ=255)
                                                                                                 cTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAAcgt                                                     >  1:1720661/1‑150 (MQ=255)
                                                                                                   atatCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAAcgtcg                                                   >  2:1825705/1‑150 (MQ=255)
                                                                                                         aaaCGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAACGTCGTATTaa                                             >  2:3757455/1‑150 (MQ=255)
                                                                                                          aaCGATTGGTTTTTAAAAAAGAAAAGAAAAAAATATCATAAAACCCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAACGTCGTATTaaa                                            <  2:768149/150‑1 (MQ=255)
                                                                                                          aaCGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAACGTCGTATTaaa                                            >  2:868536/1‑150 (MQ=255)
                                                                                                          aaCGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAACGTCGTATTaaa                                            >  1:1112813/1‑150 (MQ=255)
                                                                                                           aCGATTGGGTTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAACGTCGTATTAAac                                           <  1:4048503/150‑1 (MQ=255)
                                                                                                           aCGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAACGTCGTATTAAac                                           >  2:3899906/1‑150 (MQ=255)
                                                                                                              aTTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAACGTCGTATTAAACACt                                        >  2:3578543/1‑150 (MQ=255)
                                                                                                              aTTGGGCTTAATAACAGAAAAGAAGAAAATATCATAAAACCCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAACGTCGTATTAAACACt                                        <  1:1799925/150‑1 (MQ=255)
                                                                                                                tGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAACGTCGTATTAAACACTGc                                      >  1:2212828/1‑150 (MQ=255)
                                                                                                                 gggCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAACGTCGTATTAAACACTGCa                                     >  2:1934926/1‑150 (MQ=255)
                                                                                                                   gttttAAAACAAAAAAGAAAAAAATATCAAAAAACCCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAACGTCGTATTAAACACTGCAtt                                   <  2:15564/148‑1 (MQ=255)
                                                                                                                   gCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAACGTCGTATTAAACACTGCAtt                                   >  1:1960373/1‑150 (MQ=255)
                                                                                                                    cTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAACGTCGTATTAAACACTGCATTc                                  >  1:508813/1‑150 (MQ=255)
                                                                                                                     tttATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAACGTCGTATTAAACACTGCATTCg                                 >  2:624922/1‑150 (MQ=255)
                                                                                                                     tttATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAACGTCGTATTAAACACTGCATTCg                                 >  2:571761/1‑150 (MQ=255)
                                                                                                                     tttATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAACGTCGTATTAAACACTGCATTCg                                 >  1:3851727/1‑150 (MQ=255)
                                                                                                                     tttATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAACGTCGTATTAAACACTGCATTCg                                 >  2:3320122/1‑150 (MQ=255)
                                                                                                                      ttATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAACGTCGTATTAAACACTGCATTCgg                                >  2:2512336/1‑150 (MQ=255)
                                                                                                                      ttATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAACGTCGTATTAAACACTGCATTCgg                                >  1:136452/1‑150 (MQ=255)
                                                                                                                      ttATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAACGTCGTATTAAACACTGCATTCgg                                >  1:1856309/1‑150 (MQ=255)
                                                                                                                       tataACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAACGTCGTATTAAACACTGCATTCGGc                               >  1:3304843/1‑150 (MQ=255)
                                                                                                                       tataACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAACGTCGTATTAAACACTGCATTCGGc                               >  2:4145206/1‑150 (MQ=255)
                                                                                                                            cAGAAAAGAAAAAAATATCACAAAACCCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAACGTCGTATTAAACACTGCATTCGGCTGGcc                          <  2:2401150/150‑1 (MQ=255)
                                                                                                                              gaaaaGCAAAAAATATCAAAAAACCCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAACGTCGTATTAAACACTGCATTCGGCTGGCCGa                        <  2:2213558/148‑1 (MQ=255)
                                                                                                                              gCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAACGTCGTATTAAACACTGCATTCGGCTGGCCGa                        >  1:252621/1‑150 (MQ=255)
                                                                                                                               aaaaGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAACGTCGTATTAAACACTGCATTCGGCTGGCCGAc                       <  1:3727083/149‑1 (MQ=255)
                                                                                                                                  aGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAACGTCGTATTAAACACTGCATTCGGCTGGCCGACTAt                    <  1:2342861/150‑1 (MQ=255)
                                                                                                                                   acaaaaaaTAACACAAAACCCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAACGTCGTATTAAACACTGCATTCGGCTGGCCGACTAtt                   <  2:248847/146‑1 (MQ=255)
                                                                                                                                    caaaaaaTTTCATAAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAACGTCGTATTAAACACTGCATTCGGCTGGCCGACTATTg                  <  2:1630897/147‑1 (MQ=255)
                                                                                                                                     aGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAACGTCGTATTAAACACTGCATTCGGCTGGCCGACTATTGc                 >  1:210594/1‑150 (MQ=255)
                                                                                                                                      gAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAACGTCGTATTAAACACTGCATTCGGCTGGCCGACTATTGCt                >  1:3735933/1‑150 (MQ=255)
                                                                                                                                      gAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAACGTCGTATTAAACACTGCATTCGGCTGGCCGACTATTGCt                <  1:2485096/150‑1 (MQ=255)
                                                                                                                                       aaaaTATCATAAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAACGTCGTATTAAACACTGCATTCGGCTGGCCGACTATTGCTg               <  1:893489/150‑1 (MQ=255)
                                                                                                                                        aaaTATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGACGTAAAACAGGCAACGTCGTATTAAACACTGCATTCGGCTGGCCGACTATTGCTGt              >  2:3505424/1‑150 (MQ=255)
                                                                                                                                             tcatcaAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAACGTCGTATTAAACACTGCATTCGGCTGGCCGACTATTGCTGTCGaca         >  1:526662/1‑150 (MQ=255)
                                                                                                                                              catcaAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAACGTCGTATTAAACACTGCATTCGGCTGGCCGACTATTGCTGTCGacac        >  1:949747/1‑150 (MQ=255)
                                                                                                                                                 aaaaaCCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAACGTCGTATTAAACACTGCATTCGGCTGGCCGACTATTGCTGTCGACACGca     <  1:1657894/149‑1 (MQ=255)
                                                                                                                                                  aaaaCCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAACGTCGTATTAAACACTGCATTCGGCTGGCCGACTATTGCTGTCGACACGcac    >  2:233741/1‑150 (MQ=255)
                                                                                                                                                   aaaCCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAACGTCGTATTAAACACTGCATTCGGCTGGCCGACTATTGCTGTCGACACGcaca   >  1:3081725/1‑150 (MQ=255)
                                                                                                                                                   aaaCCTGCCGTATCTTGCTGGAGCAGCACAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAACGTCGTATTAAACACTGCATTCGGCTGGCCGACTATTGCTGTCGACACGcaca   >  2:1292558/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CGACCGATGTCAGTGTTAATAAGGCGACGGCGAAACTCTACCCGGTGGCGAATACGCCTGCAGCGATGCTTGAACTGGGCGTTGAAGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGGCGTAGGTCGTAAAACAGCCAACGTCGTATTAAACACTGCATTCGGCTGGCCGACTATTGCTGTCGACACGCACAT  >  AP012306/1393230‑1393527

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑