| New junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | AP012306 | 3774746 = | 334 (1.090) | 5 (0.020) | 5/258 | 21.4 | 1.7% | intergenic (‑86/+36) | yjdF/fumB | conserved inner membrane protein/anaerobic class I fumarate hydratase |
| ? | AP012306 | 3774766 = | 280 (1.060) | intergenic (‑106/+16) | yjdF/fumB | conserved inner membrane protein/anaerobic class I fumarate hydratase | |||||
| Rejected: Coverage evenness skew score above cutoff. | |||||||||||
| Rejected: Frequency below/above cutoff threshold. | |||||||||||
TCCGGAGCTGGGTATGGAAGCTATCTGGAAAATCGAAGTAGAAGATTTCCCGGCGTTTATCCTGGTCGATGACAAAGGTAACGACTTCTTCCAGCAAATCGTCAACAAACAGTGCGCGAACTGCACTAAGTAACCTCTTCGGCCCAGCGCCTGGCAGCATGCTGCCAGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ < AP012306/3774914‑3774746‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cctggcagcatgctgccaggCGCTGGGCCGAAGAGGTTACTTAGTGCAGTTCGCGCACTGTTTGTTGACGATTTGCTGGAAGAAGTCGTTACCTTTGTCATCGACCAGGATAAACGCCGGGAAATCTTCTACTTCGATTTTCCAGATAGCTTCCATACCCAGCTCCGG > AP012306/3774766‑3774913 TCCGGAGCTGGGTATGGAAGCTATCTGGAAAATCGAAGTAGAAGATTTCCCGGCGTTTATCCTGGTCGATGACAAAGGTAACGACTTCTTCCAGCAAATCGTCAACAAACAGTGCGCGAACTGCACTAAGTAACCTCTTCGGCCCAGCGC > 2:3765587/1‑150 CCGGAGCTGGGTATGGAAGCTATCTGGAAAATCGAAGTAGAAGATTTCCCGGCGTTTATCCTGGTCGATGACAAAGGTAACGACTTCTTCCAGCAAATCGTCAACAAACAGTGCGCGAACTGCACTAAGTAACCTCTTCGGCCCAGCGCC > 2:3214353/1‑150 CGGAGCTGGGTATGGAAGCTATCTGGAAAATCGAAGTAGAAGATTTCCCGGCGTTTATCCTGGTCGATGACAAAGGTAACGACTTCTTCCAGCAAATCGTCAACAAACAGTGCGCGAACTGCACTAAGTAACCTCTTCGGCCCAGCGCCT > 1:1265638/1‑150 CGGAGCTGGGTATGGAAGCTATCTGGAAAATCGAAGTAGAAGATTTCCCGGCGTTTATCCTGGTCGATGACAAAGGTAACGACTTCTTCCAGCAAATCGTCAACAAACAGTGCGCGAACTGCACTAAGTAACCTCTTCGGCCCAGCGCCT < 1:1396148/150‑1 AGCTGGGTATGGAAGCTATCTGGAAAATCGAAGTAGAAGATTTCCCGGCGTTTATCCTGGTCGATGACAAAGGTAACGACTTCTTCCAGCAAATCGTCAACAAACAGTGCGCGAACTGCACTAAGTAACCTCTTCGGCCCAGCGCCTGGC < 1:1753539/150‑1 AGCTGGGTATGGAAGCTATCTGGAAAATCGAAGTAGAAGATTTCCCGGCGTTTATCCTGGTCGATGACAAAGGTAACGACTTCTTCCAGCAAATCGTCAACAAACAGTGCGCGAACTGCACTAAGTAACCTCTTCGGCCCAGCGCCTGGC > 2:3439685/1‑150 CTGGGTATGGAAGCTATCTGGAAAATCGAAGTAGAAGATTTCCCGGCGTTTATCCTGGTCGATGACAAAGGTAACGACTTCTTCCAGCAAATCGTCAACAAACAGTGCGCGAACTGCACTAAGTAACCTCTTCGGCCCAGCGCCTGGCAG < 2:421642/150‑1 TGGGTATGGAAGCTATCTGGAAAATCGAAGTAGAAGATTTCCCGGCGTTTATCCT‑GTCGATGACAAAGGTAACGACTTCTTCCAGCAAATCGTCAACAAACAGTGCGCGAACTGCACTAAGTAACCTCTTCGGCCCAGCGCCTGGCAGCA < 2:2463427/150‑1 TGGGTATGGAAGCTATCTGGAAAATCGAAGCAGAAGATTTCCCGGCGTTTATCCTGGTCGATGACAAAGGCAACGACTTCTTCCAGCAAATCGTCAACAAACAGTGCCCGAACTGCACTAAGTAACCTCTTCGGCCCAGCGCCTGGCAGC > 2:271217/1‑150 GGTATGGAAGCTATCTGGAAAATCGAAGTAGAAGATTTCCCGGCGTTTATCCTGGTCGATGACAAAGGTAACGACTTCTTCCAGCAAATCGTCAACAAACAGTGCGCGAACTGCACTAAGTAACCTCTTCGGCCCAGCGCCTGGCAGCAT > 1:2531827/1‑150 GTATGGAAGCTATCTGGAAAATCGAAGTAGAAGATTTCCCGGCGTTTATCCTGGTCGATGACAAAGGTAACGACTTCTTCCAGCAAATCGTCAACAAACAGTGCGCGAACTGCACTAAGTAACCTCTTCGGCCCAGCGCCTGGCAGCATG > 2:2480352/1‑150 TATGGAAGCTATCTGGAAAATCGAAGTAGAAGATTTCCCGGCGTTTATCCTGGTCGATGACAAAGGTAACGACTTCTTCCAGCAAATCGTCAACAAACAGTGCGCGAACTGCACTAAGTAACCTCTTCGGCCCAGCGCCTGGCAGCATGC < 1:1052059/150‑1 TGGAAGCTATCTGGAAAATCGAAGTAGAAGATTTCCCGGCGTTTATCCTGGTCGATGACAAAGGTAACGACTTCTTCCAGCAAATCGTCAACAAACAGTGCGCGAACTGCACTAAGTAACCTCTTCGGCCCAGCGCCTGGCAGCATGCTG < 2:453801/150‑1 GGAAGCTATCTGGAAAATCGAAGTAGAAGATTTCCCGGCGTTTATCCTGGTCGATGACAAAGGTAACGACTTCTTCCAGCAAATCGTCAACAAACAGTGCGCGAACTGCACTAAGTAACCTCTTCGGCCCAGCGCCTGGCAGCATGCTGC > 1:1730480/1‑150 AGCTATCTGGAAAATCGAAGTAGAAGATTTCCCGGCGTTTATCCTGGTCGATGACAAAGGTAACGACTTCTTCCAGCAAATCGTCAACAAACAGTGCGCGAACTGCACTAAGTAACCTCTTCGGCCCAGCGCCTGGCAGCATGCTACCAG > 1:1767287/1‑150 AAAGGTAACGACTTCTTCCAGCAAATCGTCAACAAACAGTGCGCGAACTGCACTAAGTAACCTCTTCGGCCCAGCGCCTGGCAGCATGCTGCCAGGCGCTGGGCCGAAGAGGTTACTTAGTGCAGTTCG < 2:3428789/129‑1 CAAATCGTCAACAAACAGTGCGCGAACTGCACTAAGTAACCTCTTCGGCCCAGCGCCTGGCAGCATGCTGCCAGGCGCTGGGCC < 2:404835/84‑1 CAAATCGTCAACAAACAGTGCGCGAACTGCACTAAGTAACCTCTTCGGCCCAGCGCCTGGCAGCATGCTGCCAGGCGCTGGGCC > 1:404835/1‑84 GTCAACAAACAGTGCGCGAACTGCACTAAGTAACCTCTTCGGCCCAGCGCCTGGCAGCATGCTGCCAGGCGCTGGGCCGAAGAGGTTACTTAGTGCAGTTCGCGCACTGTTTGTTGACGATTTGCTGGAAGAAGTCGTTACCTTTGTCAT > 2:2470844/1‑150 CGAACTGCACTAAGTAACCTCTTCGGCCCAGCGCCTGGCAGCATGCTGCCAGGCGCTGGGCCGAAGAGGTTACTTAGTGCAGTTCGCGCACTGTTTGTTGACGATTTGCTGGAAGAAGTCGTTACCTTT < 1:3428789/129‑1 AGCATGCTGCCAGGCGCTGGGCCGAAGAGGTTACTTAGTGCAGTTCGCGCACTGTTTGTTGACGATTTGCTGGAAGAAGTCGTTACCTTTGTCATCGACCAGGATAAACGCCGGGAAATCTTCTACTTCGATTTTCCAGATAGCTTCCAT < 2:875068/150‑1 GCATGCTGCCAGGCGCTGGGCCGAAGAGGTTACTTAGTGCAGTTCGCGCACTGTTTGTTGACGATTTGCTGGAAGAAGTCGTTACCTTTGTCATCGACCAGGATAAACGCCGGGAAATCTTCTACTTCGATTTTCCAGATAGCTTCCATA < 2:2412414/150‑1 GCATGCTGCCAGGCGCTGGGCCGAAGAGGTTACTTAGTGCAGCTCGCGCACTGTTTGTTGACGATTTGCTGGAAGAAGTCGTTACCTTTGTCATCGACCAGGATAAACGCCGGGAAATCTTCTACTTCGATTTTCCAGATAGCTTCCATA < 2:3566988/150‑1 ATGCTGCCAGGCGCTGGGCCGAAGAGGTTACTTAGTGCAGTTCGCGCACTGTTTGTTGACGATTTGCTGGAAGAAGTCGTTACCTTTGTCATCGACCAGGATAAACGCCGGGAAATCTTCTACTTCGATTTTCCAGATAGCTTCCATACC > 2:4128406/1‑150 ATGCTGCCAGGCGCTGGGCCGAAGAGGTTACTTAGTGCAGTTCGCGCACTGTTTGTTGACGATTTGCTGGAAGAAGTCGTTACCTTTGTCATCGACCAGGATAAACGCCGGGAAATCTTCTACTTCGATTTTCCAGATAGCTTCCATACC > 2:921174/1‑150 CCAGGCGCTGGGCCGAAGAGGTTACTTAGTGCAGTTCGCGCACTGTTTGTTGACGATTTGCTGGAAGAAGTCGTTACCTTTGTCATCGACCAGGATAAACGCCGGGAAATCTTCTACTTCGATTTTCCAGATAGCTTCCATACCCAGCTC < 1:1283225/150‑1 AGGCGCTGGGCCGAAGAGGTTACTTAGTGCAGTTCGCGCACTGTTTGTTGACGATTTGCTGGAAGAAGTCGTTACCTTTGTCATCGACCAGGATAAACGCCGGGAAATCTTCTACTTCGATTTTCCAGATAGCTTCCATACCCAGCTCCG > 2:2326878/1‑150 GGCGCTGGGCCGAAGAGGTTACTTAGTGCAGTTCGCGCACTGTTTGTTGACGATTTGCTGGAAGAAGTCGTTACCTTTGTCATCGACCAGGATAAACGCCGGGAAATCTTCTACTTCGATTTTCCAGATAGCTTCCATACCCAGCTCCGG > 1:3497502/1‑150 GGCGCTGGGCCGAAGAGGTTACTTAGTGCAGTTCGCGCACTGTTTGTTGACGATTTGCTGGAAGAAGTCGTTACCTTTGTCATCGACCAGGATAAACGCCGGGAAATCTTCTACTTCGATTTTCCAGATAGCTTCCATACCCAGCTCCGG < 1:2959777/150‑1 TCCGGAGCTGGGTATGGAAGCTATCTGGAAAATCGAAGTAGAAGATTTCCCGGCGTTTATCCTGGTCGATGACAAAGGTAACGACTTCTTCCAGCAAATCGTCAACAAACAGTGCGCGAACTGCACTAAGTAACCTCTTCGGCCCAGCGCCTGGCAGCATGCTGCCAGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ < AP012306/3774914‑3774746‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cctggcagcatgctgccaggCGCTGGGCCGAAGAGGTTACTTAGTGCAGTTCGCGCACTGTTTGTTGACGATTTGCTGGAAGAAGTCGTTACCTTTGTCATCGACCAGGATAAACGCCGGGAAATCTTCTACTTCGATTTTCCAGATAGCTTCCATACCCAGCTCCGG > AP012306/3774766‑3774913 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |