Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123061,421,1470TG26.6% 528.5 / 10.6 253T79P (ACC→CCC) ydhBpredicted DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (106/79);  new base G (67/0);  total (174/79)
Fisher's exact test for biased strand distribution p-value = 7.29e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 295 total aligned reads displayed.

TTCCTGAAAGACAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCGGCACCGCCAGCCACTCTTCCAGCTGACGCACGGTATAGCTGACCGCAGAAGGAA  >  AP012306/1420998‑1421296
                                                                                                                                                     |                                                                                                                                                     
ttCCTGAAAGACAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGt                                                                                                                                                       >  1:3431118/1‑150 (MQ=255)
  ccggAAAGACAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGa                                                                                                                                                     <  2:3250644/147‑1 (MQ=255)
  ccTGAAAGACAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGa                                                                                                                                                     >  1:3864813/1‑150 (MQ=255)
   cTGAAAGACAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGAt                                                                                                                                                    >  1:2321974/1‑150 (MQ=255)
   cTGAAAGACAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGAt                                                                                                                                                    <  2:366503/150‑1 (MQ=255)
    tGAAAGACAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTAACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATc                                                                                                                                                   <  1:2215307/150‑1 (MQ=255)
      aaaGACAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGGTCTg                                                                                                                                                 >  1:3743358/1‑150 (MQ=255)
      aaaGACAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTg                                                                                                                                                 <  2:2806711/150‑1 (MQ=255)
       aaGACAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGc                                                                                                                                                <  1:3125738/150‑1 (MQ=255)
        aGACAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCa                                                                                                                                               <  1:277471/150‑1 (MQ=255)
        aGACAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGcc                                                                                                                                               >  2:48864/1‑149 (MQ=255)
         gACAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGcct                                                                                                                                              >  2:2921401/1‑148 (MQ=255)
         gACAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCAt                                                                                                                                              <  1:1700468/150‑1 (MQ=255)
           cAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCAttt                                                                                                                                            <  2:2686579/150‑1 (MQ=255)
            aagaagTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCAtttt                                                                                                                                           <  1:1328227/150‑1 (MQ=255)
            aagaagTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCAtttt                                                                                                                                           >  2:3064007/1‑150 (MQ=255)
            aagaagTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGCGTTCTGCAtttt                                                                                                                                           >  1:1910204/1‑150 (MQ=255)
             agaagTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGGTCTGCAttttt                                                                                                                                          >  1:1045373/1‑150 (MQ=255)
             agaagTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGGTCTGCAttttt                                                                                                                                          >  2:940486/1‑150 (MQ=255)
                agTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTg                                                                                                                                       <  2:3388362/150‑1 (MQ=255)
                   ccGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGaca                                                                                                                                    <  2:1780607/149‑1 (MQ=255)
                    cGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTTacaa                                                                                                                                   >  2:2543198/1‑150 (MQ=255)
                    cGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGacaa                                                                                                                                   <  1:530717/150‑1 (MQ=255)
                     gACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTTacaac                                                                                                                                  >  1:1155559/1‑150 (MQ=255)
                      aCATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGacaaca                                                                                                                                 <  2:1038412/150‑1 (MQ=255)
                      aCATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCATTTTTTTTaaaaca                                                                                                                                 >  2:1412298/1‑150 (MQ=255)
                       catcatCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACag                                                                                                                                <  2:2722125/150‑1 (MQ=255)
                       catcatCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACag                                                                                                                                <  1:1359100/150‑1 (MQ=255)
                         tcatcaAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACagag                                                                                                                              <  1:410195/150‑1 (MQ=255)
                             caAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGGTCTGCCTTTTTTTTACAAAAAAGGGcc                                                                                                                          >  2:3649494/1‑150 (MQ=255)
                               aaaTGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCct                                                                                                                        <  1:1016630/150‑1 (MQ=255)
                               aaaTGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCATTTTTTTTACAACAGAGCGCCct                                                                                                                        >  2:1591774/1‑150 (MQ=255)
                                 aTGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGGGCGCCcttc                                                                                                                      >  1:3221775/1‑150 (MQ=255)
                                  tGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCcttct                                                                                                                     >  1:2252218/1‑150 (MQ=255)
                                  tGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCATTTTTTTTACAACAAAACGCCcttct                                                                                                                     >  1:1121927/1‑150 (MQ=255)
                                  gggCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGTCCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCcttct                                                                                                                     <  2:109212/149‑1 (MQ=255)
                                   ggCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCcttctt                                                                                                                    <  2:3146036/150‑1 (MQ=255)
                                    gCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCttt                                                                                                                   <  1:3229594/150‑1 (MQ=255)
                                      gATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTTACAACAAAGCGCCCTTCtttta                                                                                                                 >  2:1496246/1‑148 (MQ=255)
                                      gATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTga                                                                                                                 <  1:3823756/150‑1 (MQ=255)
                                       aTAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTgag                                                                                                                <  1:1817609/150‑1 (MQ=255)
                                        tAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCATTTTTTTTACAACAGAGCGCCCTTCTTTtaga                                                                                                               >  1:2426308/1‑150 (MQ=255)
                                          aaaaTCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATGTTCATTTTTTTTTCAACAAAGAGCCCTTCTTTgcgcaa                                                                                                             >  2:71018/1‑145 (MQ=255)
                                          aaaaTCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTTACAACAGAGCGCCCTTCTTTGAGaaa                                                                                                             >  1:1493553/1‑150 (MQ=255)
                                          aaaaTCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGaaa                                                                                                             <  1:2372606/150‑1 (MQ=255)
                                          aaaaTCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGaaa                                                                                                             <  1:3353453/150‑1 (MQ=255)
                                          aaaaTCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCATTTTTTTTGCAACAGAGGGCCCCTCTTTTAGaaa                                                                                                             >  2:901635/1‑150 (MQ=255)
                                          aaaaTCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGaaa                                                                                                             >  2:3883440/1‑150 (MQ=255)
                                           aaaTCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTTACAACAGAGCGCCCTTCTTTTTGAAAc                                                                                                            >  2:661305/1‑150 (MQ=255)
                                             aTCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGGTCTGCATTTTTTTGACAACAGAACGCCCCTCTTTGAGAAACCa                                                                                                          >  2:2489611/1‑150 (MQ=255)
                                             aTCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTTACAACAGAGGGCCCTTCTTTTAGAAAACa                                                                                                          >  2:734317/1‑150 (MQ=255)
                                               cAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCATTTTTTTTGCAACAGAACGCCCCTCTTTGAGAAAACACg                                                                                                        >  2:1703945/1‑150 (MQ=255)
                                                    atcatcTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTTCATTTTTTTTTCAACAAAACGGCCTTCTTTGAGGAACCACCCCCca                                                                                                   >  2:601808/1‑150 (MQ=255)
                                                    atcatcTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTTACAACAGAGCGCCCTTCTTTTAGAAACCACGCTCca                                                                                                   >  2:4004986/1‑150 (MQ=255)
                                                    atcatcTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCca                                                                                                   >  1:2865426/1‑150 (MQ=255)
                                                    atcatcTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCCTTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCca                                                                                                   >  1:3160040/1‑150 (MQ=255)
                                                    atcatcTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCA‑TTTTTTGACAACAGAGCGCCCCTCTTTGTGAAACCACGCCCcag                                                                                                  >  2:2422172/1‑150 (MQ=255)
                                                     tcatcTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTTACAACAGAGCGCCCTTCTTTTAGAAACCACCCTCcaa                                                                                                  >  1:2170295/1‑149 (MQ=255)
                                                     tcatcTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCATTTTTTTGACAACCTAGAGCCCCTCTTTGTGAAACCACCCTCcag                                                                                                  >  1:3588463/1‑150 (MQ=255)
                                                      catcTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGGTCTGCATTTTTTTTACAACAAAGGGCCCTTCTTTGAGAAACCACGCTCcagc                                                                                                 >  2:2218611/1‑150 (MQ=255)
                                                      catcTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTTAGAAACCACGCTCcagc                                                                                                 >  1:4030911/1‑150 (MQ=255)
                                                           gCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCgg                                                                                            >  1:3770717/1‑150 (MQ=255)
                                                            ccGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTTGCAACAGAGCGCCCCTCTTTGTGAAACAACGCTCCAGCAGCGGt                                                                                           >  1:143787/1‑150 (MQ=255)
                                                               tgtACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCctt                                                                                                                       <  1:2634639/119‑1 (MQ=255)
                                                               tgtACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCctt                                                                                                                       >  2:2634639/1‑119 (MQ=255)
                                                                gtACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGc                                                                                       <  2:2881917/150‑1 (MQ=255)
                                                                     ttCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCcac                                                                                  <  1:107639/150‑1 (MQ=255)
                                                                      tCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTTACAACAGAGCGCCCTTCTTTTAGAAACCACGCTCCAGCAGCGGTCAGCTCcaca                                                                                 >  1:873578/1‑150 (MQ=255)
                                                                        tGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATc                                                                               <  1:610502/150‑1 (MQ=255)
                                                                          gCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGGTCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCAc                                                                             >  1:3604724/1‑150 (MQ=255)
                                                                              gACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTg                                                                         >  1:1464218/1‑150 (MQ=255)
                                                                                cAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGGTCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGcc                                                                       >  1:3662096/1‑150 (MQ=255)
                                                                                  atatTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTTACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCcgt                                                                     >  1:1592839/1‑150 (MQ=255)
                                                                                  atatTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCcgt                                                                     >  1:393795/1‑150 (MQ=255)
                                                                                   tattatCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATGTGCATTTTTTTGACAACAGAGCGGCCTTCTTTGAGAAAACACGCTCCAGCAGCGGGCAGGTCCACATCACGGGGCcgtc                                                                    >  2:2332518/1‑150 (MQ=255)
                                                                                    attatCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGGTCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCcgtcg                                                                   >  2:1225964/1‑150 (MQ=255)
                                                                                     ttatCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCCTTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACCGTGCcgtcgt                                                                  >  2:2143619/1‑150 (MQ=255)
                                                                                          cACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCATTTTTTTTACAAAAAAGCGCCCCTCTTTTAGAAAACACCCCCCAGCAGCGGGCAGCCCCACAACACCGGGCCGTCGGTCaaa                                                             >  2:1986734/1‑150 (MQ=255)
                                                                                             tGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAagag                                                          <  1:1966264/150‑1 (MQ=255)
                                                                                              gCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGc                                                         <  1:948055/150‑1 (MQ=255)
                                                                                                    gCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCGGCAcc                                                   <  2:767978/150‑1 (MQ=255)
                                                                                                     cTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTTAGAAACCACGCCCCAGCAGCGGGCAGCTCCCCATCACGGTGCCGTCGTTCAAAGAGCGGCAccc                                                  >  2:355606/1‑149 (MQ=255)
                                                                                                       aaCTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTTAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCGGCACCgcc                                                >  1:3379450/1‑150 (MQ=255)
                                                                                                        aCTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCCCGGTGCCGTCGTTCAAAGAGCGGCACCgcca                                               <  2:1858901/150‑1 (MQ=255)
                                                                                                         cTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCGGCACCgccag                                              <  1:503993/150‑1 (MQ=255)
                                                                                                              cgcgCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGAGATCTGCATTTTTTTGACAACAAAGCGCCCCTCTTTTTGAAAACACCCCCCAGCAGCGGTCACCTCCACACCACAGGGCCCGCGGTCAAAGAGCCGCACCCCCAGCCAcc                                         >  2:2381753/1‑149 (MQ=255)
                                                                                                                cgcCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGGTCTGCATTTTTTTGGCAAAAAAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGGCAGCTCCACATCACGGGGCCGTCGTTCAAAGAGCGGCACCGCCAGCCActct                                       >  2:2050880/1‑150 (MQ=255)
                                                                                                                  ccAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTTACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACCGTGCCGTCGTTCAAAGAGCGGCACCGCCAGCCACTCTTc                                     >  1:2520268/1‑150 (MQ=255)
                                                                                                                  ccAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCCTTTTTTTTACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACCGTGCCGTCGTTCAAAGAGCGGCACCGCCAGCCACTCTTc                                     >  2:2094120/1‑150 (MQ=255)
                                                                                                                         ttCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCATTTTTTTTACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGGCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCGGCACCGCCAACCACCCTTCCAGCTGa                              >  2:427806/1‑150 (MQ=255)
                                                                                                                           cGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCGGCACCGCCAGCCACTCTTCCAGCTGACg                            >  1:3273009/1‑150 (MQ=255)
                                                                                                                              tATCTGCTGACATTGCTGGCGGGGGGTCTGCATTTTTTTTACAAAAAAGCGCCCCTCTTTTAGAAAACACGCCCCAGCAGCGGGCAGCTCCACAACACCGGGCCGTCGTTCAAAAAGCGGGACCCCCCGCCACCCTTCCCGCCGACGccc                         >  2:2003815/1‑148 (MQ=255)
                                                                                                                                  tgctgACATTGCTGGCGGGTGATCTGCATTTTTTTTACAACAAAGCGCCCTTCTTTGAGAAACCACGCCCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAAAGCGGCACCGCCAGCCACTCTTCCAGCTGACGCACGGta                     >  2:1674748/1‑150 (MQ=255)
                                                                                                                                   gctgACATTGCTGGCGGGTGATCTGCATTTTTTTTACAACAGAGCGCCCCTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCGGCACCGCCAGCCCCTCTTCCACCTGACGCACGGtat                    >  2:809752/1‑150 (MQ=255)
                                                                                                                                       aCATTGCTGGCGGGGGATCTGCATTTTTTTTACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCGGCACCGCCAGCCACTCCTCCAGCTGACGCACGGTATAGCt                >  2:4093714/1‑150 (MQ=255)
                                                                                                                                           tGCTGGCGGGGGGTCTGCATTTTTTTGACAACAGAGCGGCCTTCTTTGGGAAACCACGCTCCAGCAGCGGTCCGCTCCACATCACGGTGCCGGCGGTCAAAGAGCGGCACCGCCAGCCACTCTTCCAGCTGACGCACGGTATAGCTGAcc            >  2:1564033/1‑150 (MQ=255)
                                                                                                                                             cTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCGGCACCGCCAGCCACTCTTCCAGCTGACGCACGGTATAGCTGACCGc          >  1:572096/1‑150 (MQ=255)
                                                                                                                                             cTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCGGCACCGCCAGCCACTCTTCCAGCTGACGCACGGTATAGCTGACCGc          >  1:2295073/1‑150 (MQ=255)
                                                                                                                                              tGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCGGCACCGCCAGCCACTCTTCCAGCTGACGCACGGTATAGCTGACCGCa         >  2:1132343/1‑150 (MQ=255)
                                                                                                                                                    gTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCGGCACCGCCAGCCACTCTTCCAGCTGACGCACGGTATAGCTGACCGCAGAAGGa   >  1:2942360/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTCCTGAAAGACAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCGGCACCGCCAGCCACTCTTCCAGCTGACGCACGGTATAGCTGACCGCAGAAGGAA  >  AP012306/1420998‑1421296

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑