Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123062,212,7530AC21.5% 540.9 / 39.9 191Y241D (TAT→GAT) hcaTpredicted 3‑phenylpropionic transporter
Reads supporting (aligned to +/- strand):  ref base A (147/3);  new base C (0/41);  total (147/44)
Fisher's exact test for biased strand distribution p-value = 1.37e-38
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 248 total aligned reads displayed.

CAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCG‑CCA‑CCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAGCGCCAACCACGCAGACCAACCGGT  >  AP012306/2212604‑2212900
                                                                                                                                                       |                                                                                                                                                   
cAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCG‑CCA‑CCACGCCCAGCGACCACAAATa                                                                                                                                                     >  1:3717898/1‑150 (MQ=255)
 aaCGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCG‑CCA‑CCACGCCCAGCGACCACAAATAc                                                                                                                                                    >  1:536247/1‑150 (MQ=255)
         aGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCG‑CCA‑CCACGCCCAGCGACCACAAATACCCCAccgc                                                                                                                                            >  1:734421/1‑150 (MQ=255)
         aGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCG‑CCA‑CCACGCCCAGCGACCACAAATACCCCAccgc                                                                                                                                            >  1:577863/1‑150 (MQ=255)
           ttGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCG‑CCA‑CCACGCCCAGCGACCACAAATACCCCAccgccg                                                                                                                                          >  1:1445237/1‑150 (MQ=255)
                cccATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATACCGCGTGCCCTACAACGGCGAAAAAGTTTATTACTCAGCGCAAAGATAATGACTCCCG‑CCA‑CCACGCCCAGCGACCACAAATCCCCCACCgccgaggcc                                                                                                                                     <  2:159610/150‑1 (MQ=255)
                  cATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCG‑CCA‑CCACGCCCAGCGACCACAAATACCCCACCgccgaggccga                                                                                                                                   >  1:3011149/1‑150 (MQ=255)
                   aTAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCG‑CCA‑CCACGCCCAGCGACCACAAATCCCCCACCgccgaggccgag                                                                                                                                  <  1:759708/150‑1 (MQ=255)
                     aaTGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCG‑CCA‑CCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTa                                                                                                                                >  1:3627643/1‑150 (MQ=255)
                         ccccAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTCCCG‑CCA‑CCACGCCCAGCGACCACAAATCCCCCACCGCCGAGGCCGAGTAGCCa                                                                                                                            <  2:3572363/150‑1 (MQ=255)
                                gcACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGAAAAAGTTTATTCCTCAGCGAAAAGATAATGCCTCCCG‑CCC‑CCACGCCCAGCGACCACAAATCCCCCACCGCCGAGGCCGAGTAGCCAGctgcct                                                                                                                     <  2:2891414/150‑1 (MQ=255)
                                 cACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAAAGACTTCCG‑CCA‑CCACGCCCAGCGACCACAAATACCCCACCGCCGCAGCCAAGTAACCAcctgcctg                                                                                                                    >  2:202270/1‑150 (MQ=255)
                                  aCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCG‑CCA‑CCACGCCCAGCGACCACAAATACCCCACCGCCCAGGCCGAGTATCCAGctgcctgc                                                                                                                   >  1:283929/1‑150 (MQ=255)
                                   cTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCG‑CCA‑CCACGCCCAGCGACCACAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCtgcctgcc                                                                                                                  <  1:3355480/150‑1 (MQ=255)
                                    tccgccgcAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCG‑CCA‑CCCCGCCCAGCGACCACAAATCCCCCCCCGCCGAGGCCGAGTAGCCAGCTGCCTGCCa                                                                                                                 <  2:3439679/148‑1 (MQ=255)
                                           cAAAGCGCCGAAATCAACAGCATACCGCGTGCACTACAACGGCGAAAAAGTTTATTCCCCAGCGAAAAAAAAATGACTCCCG‑CCC‑CCCCGCCCGGCGACCAAAAATCCCCCCCCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATg                                                                                                          <  2:2295842/150‑1 (MQ=255)
                                            aaaTCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCG‑CCA‑CCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATgg                                                                                                         >  2:3475277/1‑150 (MQ=255)
                                             aaTCGCCGAGATCAACAGCATATCGCGTGGAAAAGAACGGGGGAAAAGGTTATTAATGAGCGCAAAGAAAATGAGTTCCG‑CCA‑ACAAGGCCAGCGGACACAAAAAACCCAACGGCGAGGGCGAGGAGGCAGCTGCCTGCCAGTAAATggg                                                                                                        >  2:2169666/1‑149 (MQ=255)
                                              aTCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCG‑CCA‑CCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGgcg                                                                                                       >  2:219207/1‑150 (MQ=255)
                                                 gCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCG‑CCA‑CCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTa                                                                                                    >  1:3568344/1‑150 (MQ=255)
                                                 gCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCG‑CCA‑CCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTa                                                                                                    >  1:1722646/1‑150 (MQ=255)
                                                   cGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGGTTATTACTCAGCGCAAAGATAATGACTTCCG‑CCA‑CCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTaaa                                                                                                  >  1:3956631/1‑150 (MQ=255)
                                                     agaTCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCG‑CCA‑CCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAAc                                                                                                >  1:1838532/1‑150 (MQ=255)
                                                        tCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCG‑CCA‑CCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGt                                                                                             >  1:2402630/1‑150 (MQ=255)
                                                          aaCAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCG‑CCA‑CCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTaa                                                                                           >  1:3518543/1‑150 (MQ=255)
                                                           aCAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCG‑CCA‑CCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAAt                                                                                          >  1:79143/1‑150 (MQ=255)
                                                                atatCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCG‑CCA‑CCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGcc                                                                                     >  1:3402499/1‑150 (MQ=255)
                                                                      cgTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCG‑CCA‑CCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCAtgt                                                                               >  1:3413414/1‑150 (MQ=255)
                                                                      cgTGCACTACAACGGCGAAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCG‑CCA‑CCACGCCCAGCGACCACAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCAtgt                                                                               <  1:1124833/150‑1 (MQ=255)
                                                                      cgTCCACAACACGGGCGAAAAATTTTATTCCCCAGCGAAAAGAAAATGCCTCCCG‑CCC‑CCACCCCCAGCGACCAAAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCAtgt                                                                               <  2:1911447/150‑1 (MQ=255)
                                                                       gTGCACTACACCGGCGGAAAATTTTATTCCTCAGCGAAAAGAAAATGCCTCCCG‑CCC‑CCACGCCCAGCGACCAAAAATCCCCCCCCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCAtgtg                                                                              <  2:2250643/150‑1 (MQ=255)
                                                                         gcccTACACCGGCGAAAAAGTTTATTACTCAGCGCAAAGATAATGACTCCCG‑CCC‑CCACGCCCAGCGACTACAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGcc                                                                            <  2:3405955/147‑1 (MQ=255)
                                                                         gCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCG‑CCA‑CCACGCCCAGCGACCACAAATACCCCACCCCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGcc                                                                            >  2:3706093/1‑150 (MQ=255)
                                                                          cACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCG‑CCA‑CCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGccc                                                                           >  2:527283/1‑150 (MQ=255)
                                                                          cACTAAAACGGGGAAAAGGTTTATAACTCAGCGAAAAAAAAAGGCCTCCCG‑CCC‑CCCCCCCCAGCGACCAAAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGccc                                                                           <  2:785844/150‑1 (MQ=255)
                                                                           aCTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCG‑CCA‑CCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGcccc                                                                          >  2:3853049/1‑150 (MQ=255)
                                                                            cTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCG‑CCA‑CCACGCCCAGCGACCACAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGccccc                                                                         <  1:220195/150‑1 (MQ=255)
                                                                             tACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCG‑CCA‑CCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCt                                                                        >  1:865131/1‑150 (MQ=255)
                                                                              aCAACGGCGGCAAAATTTATTACTCAGCGCAAAGATAATGAATTCCG‑CCC‑CCCCGCCCTGCGCCCACAAATACCCCACCGCCGAGGCCGAGTAGCCTGCTGCCTGCCAGTAAATTGCGCTAAAACCGTAACAAGCCGCATGTGccccccg                                                                       >  2:4097513/1‑148 (MQ=255)
                                                                              aCAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCG‑CCA‑CCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTg                                                                       >  1:2170937/1‑150 (MQ=255)
                                                                                aaCGGCGAAAAAGTTTATTCCCCAGCGCAAAGATAAGGACTTCCG‑CCC‑CCCCGCCCAGCGCCCAAAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCa                                                                     <  2:2691397/150‑1 (MQ=255)
                                                                                  cggcggAAAATTTTATTCCCCAGCGAAAAGAAAATGACTCCCG‑CCA‑CCCCGCCCAGCGACCAAAAATCCCCCCCCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAAt                                                                   <  2:2330423/150‑1 (MQ=255)
                                                                                  cggcgaAAAATTTTATTCCTCAGCCAAAAGAAAATGACTCCCG‑CCC‑CCACCCCCAGCGACCACAAATCCCCCCCCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAAt                                                                   <  2:163237/150‑1 (MQ=255)
                                                                                  cggcgaAAAAGTTTATTACTCAGCGAAAAGAAAATGCCTCCCG‑CCC‑CCCCGCCCAGCGCCCACAAATCCCCCCCCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAAt                                                                   <  2:1136836/150‑1 (MQ=255)
                                                                                   ggcggAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCG‑CCA‑CCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATa                                                                  >  1:3236617/1‑150 (MQ=255)
                                                                                    gcggAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCG‑CCA‑CCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATaa                                                                 >  2:1901823/1‑150 (MQ=255)
                                                                                    gcggAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCG‑CCA‑CCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATaa                                                                 >  2:1344839/1‑150 (MQ=255)
                                                                                     cggAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCG‑CCA‑CCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATaaa                                                                >  2:1209540/1‑150 (MQ=255)
                                                                                       aaaaaTTTTATTACTCAGCGCAAAGAAAATGACTCCCG‑CAC‑CCCCGCCCAGCGACAAAAAACCCCCCCCCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATaaaca                                                              <  2:2913265/149‑1 (MQ=255)
                                                                                       aaaaaGTTTATTCCCCAGCGAAAAGAAAATGCCTCCCG‑CCC‑CCCCCCCAAGCGCCCAAAAATCCCCCCCCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATaaaca                                                              <  2:4015827/149‑1 (MQ=255)
                                                                                        aaaaGTTTATTACTCAGCGCAAAGATAATGACTTCCG‑CCA‑CCACGCCCAGCGACCACAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATaaacaa                                                             <  1:94208/150‑1 (MQ=255)
                                                                                        aaaaGTTTATTACTCAGCGCAAAGATAATGACTTCCG‑CCA‑CCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATaaacaa                                                             >  1:1955700/1‑150 (MQ=255)
                                                                                        aaaaGTTTATTACTCAGCGCAAAGATAATGACTTCCG‑CCA‑CCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATaaacaa                                                             >  2:2545867/1‑150 (MQ=255)
                                                                                         aaaTTTTATTACTCAGCGAAAAGATAATGCCTCCCG‑CCA‑CCCCCCCCAGCGCCCAAAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATaaacaaa                                                            <  2:2061059/150‑1 (MQ=255)
                                                                                         aaaGTTTATTCCCCAGCGCAAAAATAAGGACTCCCG‑CCA‑CCCCGCCCAGCGACCAAAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATaaacaaa                                                            <  2:1224773/150‑1 (MQ=255)
                                                                                         aaaGTTTATTACTCAGCGCAAAGATAATGACTTCCG‑CCA‑CCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATaaacaaa                                                            >  2:977442/1‑150 (MQ=255)
                                                                                         aaaGTTTATTACTCAGCGCAAAGATAATGACTTCCG‑CCA‑CCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATaaacaaa                                                            >  1:2963288/1‑150 (MQ=255)
                                                                                          aaGTTTATTACCCAGCGAAAAGATAATGACTTCCG‑CCC‑CCCCGCCCAGCGACCACAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATaaacaaac                                                           <  1:3373257/150‑1 (MQ=255)
                                                                                           aGTTTATTACTCAGCGCAAAGATAATGACTTCCG‑CCA‑CCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACg                                                          >  2:3901355/1‑150 (MQ=255)
                                                                                           aGTTTATTACTCAGCGCAAAGATAATGACTTCCG‑CCA‑CCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACg                                                          >  2:1689746/1‑150 (MQ=255)
                                                                                           aGTTTATTACCCAGCAAAAAGAAAATGCCTCCCC‑CCC‑CCCCCCCCAGCGCCCAAAAACCCCCCCCCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACg                                                          <  2:3313591/150‑1 (MQ=255)
                                                                                            gTTTATTCCTCAGCGCAAAAAAAATGCCTCCCG‑CCC‑CCACGCCCAGCGCCCAAAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGc                                                         <  2:2804147/150‑1 (MQ=255)
                                                                                            gTTTATTACTCAGCGCAAAGATAATGACTTCCG‑CCA‑CCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGc                                                         >  2:1399653/1‑150 (MQ=255)
                                                                                             tttATTACTCAGCGCAAAGATAATGACTTCCG‑CCA‑CCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCa                                                        >  1:3184449/1‑150 (MQ=255)
                                                                                             tttATTACTCAGCGCAAAGATAATGACTTCCG‑CCA‑CCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCa                                                        >  1:1288694/1‑150 (MQ=255)
                                                                                              ttattaCTCAGCGCAAAGATAATGACTTCCG‑CCA‑CCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAg                                                       >  1:4136612/1‑150 (MQ=255)
                                                                                              ttattaCTCAGCGCAAAGATAATGACTTCCG‑CCA‑CCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAg                                                       >  2:183314/1‑150 (MQ=255)
                                                                                              ttattaCTCAGCGCAAAGATAATGACTTCCG‑CCA‑CCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAg                                                       >  1:1552045/1‑150 (MQ=255)
                                                                                              ttattaCTCAGCGCAAAGATAATGACTTCCG‑CCA‑CCACGCCCAGCGAACACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCGATAAACAAACGCAg                                                       >  2:1270433/1‑150 (MQ=255)
                                                                                                 ttaCTCAGCGCAAAGATAATGACTTCCG‑CCA‑CCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGcc                                                    >  1:3227678/1‑150 (MQ=255)
                                                                                                    cTCAGCGCAACGATAATGAGTTCCG‑CCA‑CCACGCCCCGCGACCACAAATACCCCACCGCCGAGGCCGAGCAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGa                                                 >  2:3029931/1‑150 (MQ=255)
                                                                                                    cTCAGCGCAAAGATAATGACTTCCG‑CCA‑CCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGa                                                 >  2:3720915/1‑150 (MQ=255)
                                                                                                      cAGCGCAAAGATAATGACTTCCG‑CCA‑CCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGaaa                                               >  2:2532563/1‑150 (MQ=255)
                                                                                                         cgaaaaGATAATGCCTCCCG‑CCA‑CCACGCCCAGCGACCACAAATCCCCCCCCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAgcg                                            <  2:1460516/147‑1 (MQ=255)
                                                                                                          gcAAAGATAATGACTTCCG‑CCA‑CCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAgcgc                                           >  1:2100496/1‑150 (MQ=255)
                                                                                                              aGATAATGACTTCCG‑CCA‑CCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGt                                       >  2:750314/1‑150 (MQ=255)
                                                                                                              aGATAATGACTTCCG‑CCA‑CCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGt                                       >  1:1775850/1‑150 (MQ=255)
                                                                                                              aGATAATGACTTCCG‑CCA‑CCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGt                                       >  1:657271/1‑150 (MQ=255)
                                                                                                                aTAATGACTTCCG‑CCA‑CCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTc                                     >  1:2482770/1‑150 (MQ=255)
                                                                                                                aTAATGACTTCCG‑CCA‑CCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTc                                     >  1:1904692/1‑150 (MQ=255)
                                                                                                                  aaTGGCCTCCGCCCA‑CCACGCCCGGCGACCACAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCt                                    <  1:763104/150‑1 (MQ=255)
                                                                                                                   aTGACTCCCG‑CCA‑CCACGCCCAGCGACCACAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTgg                                  <  1:1319394/150‑1 (MQ=255)
                                                                                                                     gACTTCCG‑CCA‑CCACGCCCAGCGACCACAAATACCCCACCGCCGAGACCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTCCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCg                                >  2:1148031/1‑150 (MQ=255)
                                                                                                                      gcctCCG‑CCACCCCCGCCAAGGGCCAACAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCg                                <  1:964803/147‑1 (MQ=255)
                                                                                                                         tCCG‑CCA‑CCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAAcc                            >  1:2309177/1‑150 (MQ=255)
                                                                                                                         tCCG‑CCA‑CCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAAcc                            >  2:1925657/1‑150 (MQ=255)
                                                                                                                            g‑CCA‑CCACGCCAAGCGACCACAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAgc                         <  1:270948/150‑1 (MQ=255)
                                                                                                                                ccccacGCCCAGCCCCCAAAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAgcgc                       <  2:924424/148‑1 (MQ=255)
                                                                                                                                   cacGCCAAGCGACCAAAAATCCCCCCCCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAGCGCCaa                    <  1:263663/150‑1 (MQ=255)
                                                                                                                                       cccAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAGCGCCAACCAc                >  1:212208/1‑150 (MQ=255)
                                                                                                                                       cccAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAGCGCCAACCAc                >  2:2498604/1‑150 (MQ=255)
                                                                                                                                       cccAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAGCGCCAACCAc                >  1:1178747/1‑150 (MQ=255)
                                                                                                                                        ccAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAAAGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAGCGCCAACCACg               >  2:4169198/1‑150 (MQ=255)
                                                                                                                                         cAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAGCGCCAACCACGc              >  2:1027476/1‑150 (MQ=255)
                                                                                                                                          aGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAGCGCCAACCACGCa             >  2:3086372/1‑150 (MQ=255)
                                                                                                                                             gACCACAAATCCCCCCCCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAGCGCCAACCACGCAGAc          <  1:2028367/150‑1 (MQ=255)
                                                                                                                                             gACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAGCGCCAACCACGCAGAc          >  1:1640995/1‑150 (MQ=255)
                                                                                                                                               ccACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCTGGCCAGAAAGCGCCAGTTCTGGCGAACCAGCGCCAACCACGCAGACCa        >  2:3227323/1‑150 (MQ=255)
                                                                                                                                               ccAAAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAGCGCCAACCACGCAGACCa        <  1:1082309/150‑1 (MQ=255)
                                                                                                                                                 acaAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAGCGCCAACCACGCAGACCAAc      >  1:1409950/1‑150 (MQ=255)
                                                                                                                                                       |                                                                                                                                                   
CAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCG‑CCA‑CCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAGCGCCAACCACGCAGACCAACCGGT  >  AP012306/2212604‑2212900

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑