Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123063,324,4330TA24.1% 512.0 / 85.2 207intergenic (+2/+52)yieF/yieGchromate reductase, Class I, flavoprotein/predicted inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (131/26);  new base A (0/50);  total (131/76)
Fisher's exact test for biased strand distribution p-value = 4.73e-29
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 243 total aligned reads displayed.

GTGATGAACAAGCCGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGATTTTCA  >  AP012306/3324285‑3324582
                                                                                                                                                    |                                                                                                                                                     
gTGATGAACAAGCCGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCtaaata                                                                                                                                                      <  1:733151/150‑1 (MQ=255)
gTGATGAACAAGCCGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCtaaata                                                                                                                                                      <  2:416503/150‑1 (MQ=255)
  gatgaACAAGCCGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCtaaataaa                                                                                                                                                    >  1:3666268/1‑150 (MQ=255)
  gatgaACAAGCCGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCtaaataaa                                                                                                                                                    <  1:4074306/150‑1 (MQ=255)
     gaACAAGCCGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATaaaaaa                                                                                                                                                 <  1:3209226/150‑1 (MQ=255)
      aaCAAGCCGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAAc                                                                                                                                                >  1:1190912/1‑150 (MQ=255)
      aaCAAGCCGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAAc                                                                                                                                                <  1:3470531/150‑1 (MQ=255)
       aCAAGCCGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAAcc                                                                                                                                               <  2:3046691/150‑1 (MQ=255)
        cAAGCCGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAAccc                                                                                                                                              >  2:2817624/1‑150 (MQ=255)
        cAAGCCGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAAccc                                                                                                                                              >  1:2563078/1‑150 (MQ=255)
         aaGCCGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCg                                                                                                                                             <  1:3945447/150‑1 (MQ=255)
          aGCCGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGc                                                                                                                                            >  2:1826633/1‑150 (MQ=255)
          aGCCGGAAATTATGGGCGGCGCGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCTGGGGACGCTGGACCACCTGACCGGGCAATTGACCGCATTTCGTGAGTTCATTCAGCGAGTTAAGATCTAAATAAAAAACCCGc                                                                                                                                            >  2:894512/1‑150 (MQ=255)
            ccGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGAAATTGACCGCTTTTGGTGAGTTTATTCAGGGAGTTAAGATCTAAAAAAAAAACCCGCCa                                                                                                                                          <  2:548338/150‑1 (MQ=255)
             cGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAg                                                                                                                                         >  1:2753022/1‑150 (MQ=255)
              ggAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGc                                                                                                                                        >  2:2288270/1‑150 (MQ=255)
              ggAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGc                                                                                                                                        >  2:2145101/1‑150 (MQ=255)
              ggAATTTATGGGCGGCGTGATTCAGAACAAAGATGATCCGCAAAACGGAGTAGAGCTTGCTCCGGGTACGCTAGACCACCTGGCCGGGCAATTGACCGTATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGc                                                                                                                                        >  2:2533229/1‑150 (MQ=255)
               gAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCa                                                                                                                                       >  1:1570840/1‑150 (MQ=255)
                aaTTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCaa                                                                                                                                      >  1:963331/1‑150 (MQ=255)
                  ttttggggCGGGGTGATTAAAAACAAAGTTGATCCGCAACCCGGAGAAGTGATTGATCGGGGTACGCTGGACCACCTGACGGGGCAATGGCCCGCTTTTGGTGATTTTATTCAGGGATTTAAGATCAAAAAAAAAACCCCGCCAGCAATc                                                                                                                                    <  2:1769021/145‑1 (MQ=255)
                  tttATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATc                                                                                                                                    >  1:1452500/1‑150 (MQ=255)
                      ggggCGGCGTGATTCAGAACAAAGTTGACCCGCAAACCGGAGAAGTGATTGACGGGGGTGCGCTGGACCCCCTGACCGGGAAATTGCCCGCTTTTGGTGATTTTATTCAGCGATTTAAGATCAAAAAAAAAACCCCGCCAGCAATcatgc                                                                                                                                <  2:3772481/149‑1 (MQ=255)
                       gggCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGGGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATcatgca                                                                                                                               >  2:2930661/1‑150 (MQ=255)
                            gcgTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCg                                                                                                                          >  2:2717865/1‑150 (MQ=255)
                             cgTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCgg                                                                                                                         >  2:1595913/1‑150 (MQ=255)
                                gtttaaGAACAAAGTTGACCCGAAAACGGGAGAAGTGATTGATCAGGGTCCGCGGGACCCCCTGACCGGACAATTGCCCGCATTTGGTGATTTTATTCAGGGAGTAAAGATCAAAAAAAAAACCCCGCCAGCAATCATGCATGGCGGGtt                                                                                                                      <  2:1309895/145‑1 (MQ=255)
                                  ttaaGAAAAAAGTGGATCCGAAAACCGGAGAAGTGATTGATCAGGGTACGCGGGACCACCTGACGGGGAAATTGACCCCTTTTGGTGATTTTATTCGGGGAGTTAAGATCTAAAAAAAAACCCCGCCAGCAATCATGCATGGCGGGtttt                                                                                                                    <  2:1832498/147‑1 (MQ=255)
                                   tCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGttttt                                                                                                                   >  2:3269574/1‑150 (MQ=255)
                                           aaGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACGGGGAAATTGACCGCTTTTGGTGATTTTATTCAGCGAGTTAAGATCAAAAAAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCt                                                                                                           <  2:2085471/150‑1 (MQ=255)
                                           aaGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCt                                                                                                           >  1:1260438/1‑150 (MQ=255)
                                           aaGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGGTTTTTACGCGCt                                                                                                           >  1:4150080/1‑150 (MQ=255)
                                            aGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTa                                                                                                          >  2:2202111/1‑150 (MQ=255)
                                                  tCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGGTTTTAACGCGCTATCGAtt                                                                                                    >  1:603914/1‑150 (MQ=255)
                                                    cGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGAtttt                                                                                                  >  1:2040383/1‑150 (MQ=255)
                                                    cGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGGTTTTAACGCGCTATCGAtttt                                                                                                  >  2:2900849/1‑150 (MQ=255)
                                                     gCAAACCGGAGAAGTGATTGATCAGGGTACGTGGGACCACCTGACCGGGAAATTGACCGCTTTGGGTGAGTTTATTCAGCGATTTAAGATCTAAAAAAAAACCCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTa                                                                                                 <  2:124119/150‑1 (MQ=255)
                                                       aaaCCGGAGAAGTGATTGATCGGGGTACGCTGGCCCACCTGACCGGGAAATTGCCCGCTTTTGGTGAGTTTATTCAGGGAGTTAAGATCTAAAAAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGt                                                                                               <  2:133605/150‑1 (MQ=255)
                                                       aaaCCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAgg                                                                                               >  2:7621/1‑149 (MQ=255)
                                                       aaaCCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGGTTTTAACGCGCTATCGATTTTAGt                                                                                               >  1:4041762/1‑150 (MQ=255)
                                                        aaCCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTtgtg                                                                                              >  1:3059119/1‑150 (MQ=255)
                                                           cGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGGTTTTAACGCGCTATCGATTTTAGTGAGc                                                                                           >  1:1000257/1‑150 (MQ=255)
                                                            ggAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCg                                                                                          <  1:2719527/150‑1 (MQ=255)
                                                            ggAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCg                                                                                          >  1:2669389/1‑150 (MQ=255)
                                                               gaAGTGATTGATCAGGGTACGCTGGACCACCTGCCCGGGCAATTGCCCGCATTTGGTGATTTTATTCAGCGAGTTAAGATCAAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCg                                                                                       <  2:3523251/150‑1 (MQ=255)
                                                                aaGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGGTTTTAACGCGCCATCGATTTTTATGAGCGCCGa                                                                                      >  2:3078777/1‑150 (MQ=255)
                                                                  gTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATa                                                                                    >  1:2957358/1‑150 (MQ=255)
                                                                    gattgatCAGGGTACGCTGGACCACCTGCCCGGGCAATTGCCCGCTTTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAAAAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATaaa                                                                                  <  2:2179960/150‑1 (MQ=255)
                                                                     attgatCGGGGTACGCTGGACCCCCTGACCGGGCAATTGCCCGCTTTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAAAAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAg                                                                                 <  2:1690426/150‑1 (MQ=255)
                                                                      ttgatCGGGGTACGCTGGACCACCTGACCGGGAAATTGACCGCTTTTGGTGATTTTATTCAGCGAGTTAAGATCAAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGa                                                                                <  2:3213904/150‑1 (MQ=255)
                                                                       tcaccAGGCTCCGCTGGACCCCCTTACCGGGAATTTGCCCGCTTTTGGTCAGTTTATTCACCGAGTTAAGACCAAAAAAAAAATCCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGAc                                                                               <  2:332391/146‑1 (MQ=255)
                                                                          tCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGCTTTTTACGCGCTATCGACTTTAGTGAGCGTCGATAAAGACAAt                                                                            >  1:2409397/1‑150 (MQ=255)
                                                                           aggggaccgcgGGACCACAAGAACGGGAAATAAACCCCATTGGTTGAGTTTATCCAGGGAGTAAAAATCAAAAAAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATc                                                                           <  2:1919429/143‑1 (MQ=255)
                                                                             gggTCCGCTGGCCCACGTGACGGGAAATTTGACCGTATTTGGTGATTTTATTCAGGGAGTTAATATAAAAAAAAAAACCCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCtt                                                                         <  2:3673353/150‑1 (MQ=255)
                                                                                tACGCTGGACCACCTGACGGGGAAATTGACCGATTTTGGTGAGTTTATTCAGGGAGTTAAGATCAAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAg                                                                      <  2:1432626/150‑1 (MQ=255)
                                                                                 aCGCTGGACCACCTGACCGGGCAATTGACCGCTTTTGGTGAGTTTATTCAGCGAGTTAAGATCAAAAAAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAgg                                                                     <  1:3334508/150‑1 (MQ=255)
                                                                                 aCGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAgg                                                                     >  1:2885678/1‑150 (MQ=255)
                                                                                     tGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGGTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTTAGGATaa                                                                 >  2:4084312/1‑150 (MQ=255)
                                                                                      ggACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATaaa                                                                >  2:1736855/1‑150 (MQ=255)
                                                                                        accaccTGACCGGCAAATTGCCCGCATTTGGTGATTTTATTCAGCGAGTTAAGATCTAAAAAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAAca                                                              <  1:867311/150‑1 (MQ=255)
                                                                                          ccctggACGGGGCAATTGACCGTTTTTGGTGAGTTTATTCAGGGATTTAAAATCAAAAAAAAAACCCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAAcagc                                                            <  2:1066091/145‑1 (MQ=255)
                                                                                          caccTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAAcagc                                                            >  2:398316/1‑150 (MQ=255)
                                                                                            ccTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAAcagcag                                                          >  2:2502284/1‑150 (MQ=255)
                                                                                             cTGACGGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCAAAAAAAAAACCCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACagcagc                                                         <  1:2067047/150‑1 (MQ=255)
                                                                                                aCCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGGTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCa                                                      >  1:2282802/1‑150 (MQ=255)
                                                                                                  ggggAAATTGCCCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAAAAAAAACCCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAAc                                                    <  1:568434/149‑1 (MQ=255)
                                                                                                   gggCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACg                                                   >  2:4049395/1‑150 (MQ=255)
                                                                                                   gggCAATTGACCGAATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAAAAAAAACCCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACg                                                   <  1:447659/150‑1 (MQ=255)
                                                                                                     gCAATGGACCGCTTTTGGTGAGTTTATTCAGCGATTAAAGATCAAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACgat                                                 <  2:475992/150‑1 (MQ=255)
                                                                                                         ttGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGGTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGAtt                                             >  2:2684150/1‑150 (MQ=255)
                                                                                                          tGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCAAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTa                                            <  1:3773736/150‑1 (MQ=255)
                                                                                                           gcccGCTTTTGGGGTGTTTATCCAGGGAGTTAAGATCAAAAAAAAAACCCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTAc                                           <  2:3828477/148‑1 (MQ=255)
                                                                                                               gaaTTTGGTGAGTTTATTCGGCAAGTTAAGATCAAATAAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCAc                                       <  1:1638079/148‑1 (MQ=255)
                                                                                                                 aTTTGGTGAGTTTATTCGGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACgg                                     >  2:814679/1‑150 (MQ=255)
                                                                                                                  tttGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACggg                                    >  1:686134/1‑150 (MQ=255)
                                                                                                                   ttGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGc                                   >  2:945317/1‑150 (MQ=255)
                                                                                                                   ttGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGc                                   >  2:2839148/1‑150 (MQ=255)
                                                                                                                    tGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCt                                  >  1:2362803/1‑150 (MQ=255)
                                                                                                                     ggTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCtt                                 >  1:1598823/1‑150 (MQ=255)
                                                                                                                      gTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTa                                >  1:2131534/1‑150 (MQ=255)
                                                                                                                       tGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAAACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAg                               >  1:1325653/1‑150 (MQ=255)
                                                                                                                         aGTTTATTCAGCGAGTTAAGATCTAAAAAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGAt                             <  2:1598038/150‑1 (MQ=255)
                                                                                                                            ttATTCAGGGAGTTAAGATCAAAAAAAAAACCCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCAc                          <  2:2362093/150‑1 (MQ=255)
                                                                                                                            ttATTCAGGGAGTTAAGATCAAAAAAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCAc                          <  2:3427608/150‑1 (MQ=255)
                                                                                                                            ttATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCAc                          >  1:3337900/1‑150 (MQ=255)
                                                                                                                              atccAGCGAGTAAAGATCAAAAAAAAAACCCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGc                        <  1:4108216/147‑1 (MQ=255)
                                                                                                                                tCAGCGATTTAAGATAAAAAAAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAg                      <  2:1142530/150‑1 (MQ=255)
                                                                                                                                 cAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGa                     >  1:3984615/1‑150 (MQ=255)
                                                                                                                                 cAGCGAGTTAAGATCTAAAAAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGa                     <  1:3847016/150‑1 (MQ=255)
                                                                                                                                     gAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAAAAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTc                 >  2:3453611/1‑150 (MQ=255)
                                                                                                                                     gAGTAAAGATCTAAAAAAAAACCCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTc                 <  1:1882938/150‑1 (MQ=255)
                                                                                                                                      atttAAAATCAAAAAAAAAACCCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTcc                <  2:1105652/148‑1 (MQ=255)
                                                                                                                                       gTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGGTTTTTACGCGCTATCGATTTTAGTGAGCGGTGGTAAAGAACATCTTCAGGGTAAAAAACAGCGCAACGATGGATACGCGCGGGGTTAAATCACGCAGAccgccg               >  2:1007599/1‑145 (MQ=255)
                                                                                                                                         tAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGGGAGCGTCGATAAAGACAATCTTCAGGATAAACACCAACGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCCGt             >  2:1446731/1‑150 (MQ=255)
                                                                                                                                          aaGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTa            >  1:3600199/1‑150 (MQ=255)
                                                                                                                                           aGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGGGCGTCGATAAAGAAAATCTTCAGGATAAACAGCAACGCAACGAAGATTACGAACGGGGTTAGATCACGCACGCGTCCGGTAc           >  2:802728/1‑150 (MQ=255)
                                                                                                                                            gATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGGTTTTAACGCGCTATCGATTTTAGTGAGCGGCGATAAAGACAAACTTCAGGATAAACAGCAACGCAACGATGATTAAGCACGGGCTTAGATCACGCAGACGCCCGGGAcc          >  2:1079704/1‑150 (MQ=255)
                                                                                                                                             aTCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTTACGCCCCATCGAATTTAATGAGCGTCGATAAAAACAAACTTCAGGATAAACAACAACGCAACGAAGATTACGCACGGGCCTAGATAACGCAAACCTCCGGGAccc         >  2:2018179/1‑149 (MQ=255)
                                                                                                                                                 aaaaaaaaaaCCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGAttt     <  1:1749053/150‑1 (MQ=255)
                                                                                                                                                    taaaAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTCAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGATTTtca  >  2:3519929/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GTGATGAACAAGCCGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGATTTTCA  >  AP012306/3324285‑3324582

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑