Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123063,527,3680AC29.4% 521.1 / 55.1 232L140F (TTA→TTC) rhaSDNA‑binding transcriptional activator, L‑rhamnose‑binding
Reads supporting (aligned to +/- strand):  ref base A (138/25);  new base C (0/68);  total (139/93)
Fisher's exact test for biased strand distribution p-value = 8.34e-38
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 286 total aligned reads displayed.

CGATTTCAGTTTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAG‑ATGGAACAGCAGGAAGGGGAAAATG‑ATTT‑ACCCTCGA‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAGGTGAATT  >  AP012306/3527219‑3527516
                                                                                                                                                        |                                                                                                                                                     
cGATTTCAGTTTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAG‑ATGGAACAGCAGGAAGGGGAAAATG‑ATTT‑a                                                                                                                                                       >  1:2237767/1‑150 (MQ=255)
   tttCAGTTTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAG‑ATGGAACAGCAGGAAGGGGAAAATG‑ATTT‑Accc                                                                                                                                                    >  2:3532126/1‑150 (MQ=255)
    ttCAGTTTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAG‑ATGGAACAGCAGGAAGGGGAAAATG‑ATTT‑ACCCt                                                                                                                                                   <  1:2283896/150‑1 (MQ=255)
     tCAGTTTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAG‑ATGGAACAGCAGGAAGGGGAAAATG‑ATTT‑ACCCTc                                                                                                                                                  >  2:2961942/1‑150 (MQ=255)
     tCAGTTTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAG‑ATGGAACAGCAGGAAGGGGAAAATG‑ATTT‑ACCCTc                                                                                                                                                  >  2:1256345/1‑150 (MQ=255)
      cAGTTTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAG‑ATGGAACAGCAGGAAGGGGAAAATG‑ATTT‑ACCCTCg                                                                                                                                                 >  1:4239085/1‑150 (MQ=255)
        gTTTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAG‑ATGGAACAGCAGGAAGGGGAAAATG‑ATTT‑ACCCTCGA‑c                                                                                                                                              <  2:3009221/150‑1 (MQ=255)
         tttCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAG‑ATGGAACAGCAGGAAGGGGAAAATG‑ATTT‑ACCCTCGA‑cc                                                                                                                                             <  1:367158/150‑1 (MQ=255)
         tttCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAG‑ATGGAACAGCAGGAAGGGGAAAATG‑ATTT‑ACCCTCGA‑cc                                                                                                                                             >  1:1163077/1‑150 (MQ=255)
                cggggCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAG‑ATGGAACAGCAGGAAGGGGAAAATG‑ATTT‑ACCCTCGA‑CCGCCAGTc                                                                                                                                      <  1:480344/148‑1 (MQ=255)
                ccGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAG‑ATGGAACAGCAGGAAGGGGAAAATG‑ATTT‑ACCCTCGA‑CCGCCAGTc                                                                                                                                      <  1:575035/150‑1 (MQ=255)
                 cGGGCGGATTCTGTTCCTGCCACAGGAGGTGGATGGGCAGTACCCGTCTCGCTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTCGCACAG‑AGGGAACAGAAGGAAGGGAAAAATG‑ATTT‑CCCCTCGA‑CCGCCAGTcg                                                                                                                                     <  2:2555929/150‑1 (MQ=255)
                    gCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAG‑ATGGAACAGCAGGAAGGGGAAAATG‑ATTT‑ACCCTCGA‑CCGCCAGTCGCga                                                                                                                                  >  1:2351946/1‑150 (MQ=255)
                     cTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAG‑ATGGAACAGCAGGAAGGGGAAAATG‑ATTT‑ACCCTCGA‑CCGCCAGTCGCgag                                                                                                                                 >  2:3660924/1‑150 (MQ=255)
                        aaTCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAG‑ATGGAACAGCAGGAAGGGGAAAATG‑ATTT‑ACCCTCGA‑CCGCCAGTCGCGAGATc                                                                                                                              >  2:3329207/1‑150 (MQ=255)
                        aaTCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAG‑ATGGAACAGCAGGAAGGGGAAAATG‑ATTT‑ACCCTCGA‑CCGCCAGTCGCGAGATc                                                                                                                              >  2:2587462/1‑150 (MQ=255)
                         aTCAGTTGCTGCCAAAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAG‑ATGGAACAGCAGGAAGGGGAAAATG‑ATTT‑CCCCTCGA‑CCGCCAGTCGCGAGATCt                                                                                                                             <  1:347567/150‑1 (MQ=255)
                            tgTTGCTGCCACAAGGGCTGGATGGGCAGTATCCATCTCGCGGGCGCGTTACCCACCGGGTTTTGGAGCAGGTGCGACAGCGGTTCGCACAG‑ATGGAACAGAGGGAGGGGAAAAATG‑ATTT‑ACCCGCGG‑CCGCCAGTCGCGAGATCTTGt                                                                                                                          <  2:2910987/149‑1 (MQ=255)
                            aGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAG‑ATGGAACAGCAGGAAGGGGAAAATG‑ATTT‑ACCCTCGA‑CCGCCAGTCGCGAGATCTTGt                                                                                                                          >  2:3929831/1‑150 (MQ=255)
                            aGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAG‑ATGGAACAGCAGGAAGGGGAAAATG‑ATTT‑ACCCTCGA‑CCGCCAGTCGCGAGATCTTGt                                                                                                                          >  1:289846/1‑150 (MQ=255)
                             gTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAG‑ATGGAACAGCAGGAAGGGGAAAATG‑ATTT‑CCCCTCGA‑CCGCCAGTCGCGAGATCTTGtt                                                                                                                         <  1:851031/150‑1 (MQ=255)
                             gTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAG‑ATGGAACAGCAGGAAGGGGAAAATG‑ATTT‑ACCCTCGA‑CCGCCAGTCGCGAGATCTTGtt                                                                                                                         >  1:4420623/1‑150 (MQ=255)
                               tgctgcCAAAAGAGCTGGAGGGGCATTTTCCGTCTCACTGGCGCTTTACCCACAGCGTATTGCAGCGGGTGCGACAGCTGGTTGCACAG‑ATGGACCAGCAGGAAGGGGAAAATG‑TTTT‑CCCCTCGA‑CCGCCAGTCGCGAGATCTTGTTTa                                                                                                                       <  2:4079917/150‑1 (MQ=255)
                                gctgcCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAG‑ATGGAACAGCAGGAAGGGGAAAATG‑ATTT‑ACCCTCGA‑CCGCCAGTCGCGAGATCTTGTTTAt                                                                                                                      <  1:2276499/150‑1 (MQ=255)
                                cctgcCACAAGGGCTGGAGGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAG‑ATGGAACAGCAGGAAGGGAAAAATG‑ATTT‑CCCCTCGA‑CCGCCAGTCGCGAGATCTTGTTTAt                                                                                                                      <  2:775423/149‑1 (MQ=255)
                                    ccACAAGAGCTGGATGGGCAGTACCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAG‑ATGGAACAGCAGGAAGGGGAAAATG‑ATTT‑CCCCTCGA‑CCGCCAGTCGCGAGATCTTGTTTATGCaa                                                                                                                  <  1:3984719/150‑1 (MQ=255)
                                     cacaGGAGAGGGAGGGCCAGTCCCCGTCTCAGTGGGGTGTAACCAACAGCTTATTGCAGCAGGTGCGACTGGTGGTTCAACAG‑TTGAAAAAGCAGGAGGGGGAAAATA‑TTTT‑CCCCGCAA‑CCGCCAGTCGCGAGATCTTGTTTATGCAAt                                                                                                                 <  2:3965668/150‑1 (MQ=255)
                                     cacaAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAG‑ATGGAACAGCAGGAAGGGGAAAATG‑ATTT‑CCCCTCGA‑CCGCCAGTCGCGAGATCTTGTTTATGCAAt                                                                                                                 <  1:263809/150‑1 (MQ=255)
                                      acaAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAG‑ATGGAACAGCAGGAAGGGGAAAATG‑ATTT‑ACCCTCGA‑CCGCCAGTCGCGAGATCTTGTTTATGCAAtt                                                                                                                >  1:1104609/1‑150 (MQ=255)
                                         agagCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTGGCACAG‑ATGGAACAGCAGGAAGGGGAAAATG‑ATTT‑CCCCTCGA‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACt                                                                                                             <  1:1367089/150‑1 (MQ=255)
                                          gagCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAG‑ATGGAACAGCAGGAAGGGGAAAATG‑ATTT‑ACCCTCGA‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACTg                                                                                                            >  2:3086965/1‑150 (MQ=255)
                                           agCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAG‑ATGGAACAGCAGGAAGGGGAAAATG‑ATTT‑ACCCTCGA‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGc                                                                                                           >  2:4005481/1‑150 (MQ=255)
                                              tGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAG‑ATGGAACAGCAGGAAGGGGAAAATG‑ATTT‑CCCCTCGA‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGctct                                                                                                        <  1:3717659/150‑1 (MQ=255)
                                              tGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAG‑ATGGAACAGCAGGAAGGGGAAAATG‑ATTT‑ACCCTCGA‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGctct                                                                                                        >  2:444698/1‑150 (MQ=255)
                                               ggATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAG‑ATGGAACAGCAGGAAGGGGAAAATG‑ATTT‑CCCCTCGA‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCtt                                                                                                       <  1:3865586/150‑1 (MQ=255)
                                               ggAGGGGCAGTACCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTCCACAG‑ATGGAACAGCAGGAAGGGGAAAATA‑TTTT‑CCCCTCGA‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCtt                                                                                                       <  2:3403748/150‑1 (MQ=255)
                                                  tGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAG‑ATGGAACAGCAGGAAGGGGAAAATG‑ATTT‑ACCCTCGA‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTtgct                                                                                                    >  1:4168498/1‑150 (MQ=255)
                                                    ggCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAG‑ATGGAACAGCAGGAAGGGGAAAATG‑ATTT‑ACCCTCGA‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTtgctgc                                                                                                  >  1:172050/1‑150 (MQ=255)
                                                     gCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAG‑ATGGAACAGCAGGAAGGGGAAAATG‑ATTT‑ACCCTCGA‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCg                                                                                                 >  2:3644766/1‑150 (MQ=255)
                                                         tATCCGTCTCACTGGCGCGTTAACCAAAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAG‑ATGGAACAGCAGGAAGGGGAAAATG‑ATTT‑CCCCTCGA‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTaaa                                                                                             <  2:206672/150‑1 (MQ=255)
                                                          acccGTCCCACTGGCGCTTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAG‑ATGGAACAGCAGGAAGGGAAAAATG‑ATTT‑CCCCTGGA‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTaaaa                                                                                            <  2:3587389/148‑1 (MQ=255)
                                                                ctcACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAG‑ATGGAACAGCAGGAAGGGGAAAATG‑ATTT‑ACCCTCGA‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGtt                                                                                      >  2:4384360/1‑150 (MQ=255)
                                                                ctcACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAG‑ATGGAACAGCAGGAAGGGGAAAATG‑ATTT‑ACCCTCGA‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGtt                                                                                      >  2:3251899/1‑150 (MQ=255)
                                                                 tcACTGGCGCGTAAACACCAGTGTATTGCAGCAGGTGTCACAGCTGGTCGCACAG‑ATGGAACAGCAGGAAGGGGAAAATG‑TTTT‑CCCCTCGA‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGttt                                                                                     <  2:2362675/150‑1 (MQ=255)
                                                                  cACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAG‑ATGGAACAGCAGGAAGGGGAAAATG‑ATTT‑ACCCTCGA‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTg                                                                                    <  1:515577/150‑1 (MQ=255)
                                                                   aCTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAG‑ATGGAACAGCAGGAAGGGGAAAATG‑ATTT‑CCCCTCGA‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGc                                                                                   <  2:2675896/150‑1 (MQ=255)
                                                                     tGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAG‑ATGGAACAGCAGGAAGGGGAAAATG‑ATTT‑ACCCTCGA‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAg                                                                                 >  2:635596/1‑150 (MQ=255)
                                                                     tGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAG‑ATGGAACAGCAGGAAGGGGAAAATG‑ATTT‑ACCCTCGA‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAg                                                                                 >  2:2640413/1‑150 (MQ=255)
                                                                        cggtttAACCACGGGTTATTGAAGCGGGTGCGACGGCT‑GTTGGCCAGAATGAACCAGAAGAAAGGGAAAAATG‑ATTT‑CCCCTCGA‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGAAGTTTGCAGgag                                                                              <  2:4328680/146‑1 (MQ=255)
                                                                         gcgTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAG‑ATGGAACAGCAGGAAGGGGAAAATG‑ATTT‑ACCCTCGA‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGgaga                                                                             >  2:226743/1‑150 (MQ=255)
                                                                               cccACAGCTTATTGCAGCAGGTGCGACAGCTGGTTGCACAG‑ATGGAACAGCAGGAGGGGGAAAATG‑ATTT‑CCCCTCGA‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTgg                                                                       <  1:4062304/149‑1 (MQ=255)
                                                                               aCCACAGCTTATTGCAGCAGGTGCGACAGCTGGTTGCACAG‑ATGGAACAGCAGGAAGGGGAAAATG‑ATTT‑CCCCTCGA‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTgg                                                                       <  1:4197671/150‑1 (MQ=255)
                                                                                 cacaGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAG‑ATGGAACAGCAGGAAGGGGAAAATG‑ATTT‑ACCCTCGA‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGaa                                                                     >  1:756585/1‑150 (MQ=255)
                                                                                  acaGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAG‑ATGGAACAGCAGGAAGGGGAAAATG‑ATTT‑ACCCTCGA‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGaaa                                                                    >  2:1567461/1‑150 (MQ=255)
                                                                                     gCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAG‑ATGGAACAGCAGGAAGGGGAAAATG‑ATTT‑ACCCTCGA‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACa                                                                 >  2:1585277/1‑150 (MQ=255)
                                                                                        tATTGCAGCAGGTGCGACAGCTGGTTGCACAG‑ATGGAACAGCAGGAAGGGGAAAATG‑ATTT‑ACCCTCGA‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAgcg                                                              >  1:3573292/1‑150 (MQ=255)
                                                                                            gcagcaGGTGCGACAGCTGGTTGCACAG‑ATGGAACAGCAGGAAGGGGAAAATG‑ATTT‑ACCCTCGA‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGCTTGCAGGATAACCTGGAAAACAGCGCATc                                                          >  2:828538/1‑150 (MQ=255)
                                                                                              agcagATGCGAGGGCTGTTAGCACAG‑ATGAAACAGCAGGAAGGGGAAAATG‑TTTT‑CCCCTCGA‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCAc                                                        <  1:3055703/150‑1 (MQ=255)
                                                                                                cagGTGCGACAGCTGGTTGCACAG‑ATGGAACAGCAGGAAGGGGAAAATG‑ATTT‑ACCCTCGA‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGt                                                      >  1:3504996/1‑150 (MQ=255)
                                                                                                cagGTGCGACAGCTGGTTGCACAG‑ATGGAACAGCAGGAAGGGAAAAATG‑ATTT‑CCCCTCGA‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGt                                                      <  1:3068054/150‑1 (MQ=255)
                                                                                                  ggggCGACAGCTGGTTGCACAG‑ATGGAACAGAAGGAAGGGAAAAATT‑ATTT‑CCCCTCGA‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGtct                                                    <  2:1358268/147‑1 (MQ=255)
                                                                                                    tGCGACCGCTGGTTGCACAG‑ATGGAACAGCAGGAAGGGGAAAATG‑ATTT‑CCCCTCGA‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCa                                                  <  1:2010435/150‑1 (MQ=255)
                                                                                                     gCGACAGCTGGTTGCACAG‑ATGGAACAGCAGGAAGGGGAAAATG‑ATTT‑ACCCTCGA‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCaa                                                 >  2:3287101/1‑150 (MQ=255)
                                                                                                      cGACAGCTGGTTGCACAG‑ATGGAACAGCAGGAAGGGGAAAAGG‑ATTT‑ACCCTCGA‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAAc                                                >  2:3826362/1‑150 (MQ=255)
                                                                                                       gACAGGGGGTTGCACAG‑ATGGAACAGCAGGAAGGGAAAAATG‑ATTT‑CCCCTCGA‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACt                                               <  2:1522703/150‑1 (MQ=255)
                                                                                                        aCAGCTGGTTGCACAG‑ATGGAACAGCAGGAAGGGGAAAATG‑ATTT‑CCCCTCGA‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACtt                                              <  2:2465706/150‑1 (MQ=255)
                                                                                                        aCAGCTGGTTGCACAG‑AGGGAACAGCAGGAAGGGGAAAATG‑ATTT‑CCCCTCGA‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAGCtt                                              <  1:2736801/150‑1 (MQ=255)
                                                                                                          aGCTGGTTGCACAG‑ATGGAACAGCAGGAAGGGGAAAATG‑ATTT‑ACCCTCGA‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGc                                            >  1:3343479/1‑150 (MQ=255)
                                                                                                              gtttGCACGG‑TTGGAACAGCAGAAAGGGGAAAATG‑TTTT‑ACCCGCTA‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTct                                        <  1:2471948/148‑1 (MQ=255)
                                                                                                              gtttGCACAG‑AGGGAACAGCAGGAAGGGAAAAATT‑TTTT‑CCCCTCGA‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTct                                        <  2:4038494/148‑1 (MQ=255)
                                                                                                               gTTGCACAG‑ATGGAACAGCAGGAAGGGGAAAATG‑ATTT‑ACCCTCGA‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTctg                                       >  2:1125383/1‑150 (MQ=255)
                                                                                                               gTTGCACAG‑AGGGAACAGCAGGAAGGGGAAAATG‑TTTT‑CCCCTCGA‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTctg                                       <  2:1429663/150‑1 (MQ=255)
                                                                                                               gTTGCAAAG‑ATGGAACAGCAGGAAGGGGAAAATG‑ATTT‑CCCCTCGA‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTctg                                       <  1:1843412/150‑1 (MQ=255)
                                                                                                                    acaG‑ATGGAACAGCAGGAAGGGGAAAATG‑ATTT‑ACCCTCGA‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTctggcctg                                  >  1:1209245/1‑150 (MQ=255)
                                                                                                                    acaG‑ATGGAACAGCAGGAAGGGGAAAATG‑ATTT‑ACCCTCGA‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTctggcctg                                  >  1:1754561/1‑150 (MQ=255)
                                                                                                                      aG‑ATGGAACAGCAGGAGGGGAAAAATG‑ATTT‑CCCCTCGA‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTctggcctggc                                <  2:3599967/150‑1 (MQ=255)
                                                                                                                         aaggAACAGGAGGAAGGGGAAAATG‑TTTT‑CCCCCCGACCCGCAAGT‑GCGAGATCTTGGTTATTGAAATTCTTCCCCTGGCGCGGTAAAGCAGGTTGCAGGAGAACCCGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCctggctg                              <  2:577953/148‑1 (MQ=255)
                                                                                                                           ggAACAGCAGGAAGGGGAAAATG‑ATTT‑ACCCTCGA‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTgga                            >  2:1171006/1‑150 (MQ=255)
                                                                                                                            gAACAGCAGGAAGGGAAAAATG‑ATTT‑CCCCTCGA‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTggag                           <  2:1665436/150‑1 (MQ=255)
                                                                                                                             aaCAGCAGGAAGGGGAAAATG‑ATTT‑ACCCTCGA‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTggagg                          >  1:1932818/1‑150 (MQ=255)
                                                                                                                                cagaagAAGGGGAAAAAGTATTT‑ACCGCCGA‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGAc                        <  1:1031865/145‑1 (MQ=255)
                                                                                                                                 gcagGAAGGGGAAAATG‑ATTT‑CCCCTCGA‑CCGCCAGTCGCGAGATCTTGCTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCa                      <  1:215208/150‑1 (MQ=255)
                                                                                                                                  cagGAAGGGGAAAATG‑ATTT‑CCCCTCGA‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCAt                     <  1:684650/150‑1 (MQ=255)
                                                                                                                                  cagGAAGGGGAAAATG‑ATTT‑ACCCTCGA‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCAt                     >  2:1932263/1‑150 (MQ=255)
                                                                                                                                  cagGAAGGGGAAAATG‑ATTT‑ACCCTCGA‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCAt                     >  1:2325332/1‑150 (MQ=255)
                                                                                                                                  cagGAAGGGAAAAATG‑ATTT‑ACCCTCGA‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCAt                     <  1:94311/150‑1 (MQ=255)
                                                                                                                                   agaagggggAAAATG‑ATTT‑CCCCTCGA‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCAtt                    <  2:2802081/145‑1 (MQ=255)
                                                                                                                                    ggAAGGGGAAAATG‑ATTT‑ACCCTCGA‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCAttt                   >  2:1043309/1‑150 (MQ=255)
                                                                                                                                    ggAAGGGGAAAATG‑ATTT‑ACCCTCGA‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCAttt                   >  2:3717918/1‑150 (MQ=255)
                                                                                                                                      gaaggtaaaaTG‑ATTTCACCCTGAA‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGTCTGGAGGACCAtttt                  <  2:1849978/144‑1 (MQ=255)
                                                                                                                                       aGGGGAAAATG‑ATTT‑ACCCTCGA‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGc                >  2:3458132/1‑150 (MQ=255)
                                                                                                                                       aGGGGAAAATG‑ATTT‑ACCCTCGA‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGc                >  1:1300856/1‑150 (MQ=255)
                                                                                                                                            aaaaTG‑ATTT‑CCCTTCAA‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATg           <  1:3395750/150‑1 (MQ=255)
                                                                                                                                             aaaTA‑ATTT‑CCCCTCCG‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGa          <  2:1744112/150‑1 (MQ=255)
                                                                                                                                               aTG‑ATTT‑ACCCTCGA‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAgg        >  1:772842/1‑150 (MQ=255)
                                                                                                                                                tG‑ATTT‑CCCCTCGA‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAGGt       <  2:4268791/150‑1 (MQ=255)
                                                                                                                                                 tgtttt‑CCCCTCCA‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAGGt       <  2:758346/147‑1 (MQ=255)
                                                                                                                                                 g‑ATTT‑CCCCTCGA‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAGGTg      <  2:481596/150‑1 (MQ=255)
                                                                                                                                                     tt‑ACCCTCGA‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAGGTGAAt   >  2:2138151/1‑150 (MQ=255)
                                                                                                                                                      t‑ACCCTCGA‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAGGTGAAtt  >  2:943/1‑150 (MQ=255)
                                                                                                                                                        |                                                                                                                                                     
CGATTTCAGTTTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAG‑ATGGAACAGCAGGAAGGGGAAAATG‑ATTT‑ACCCTCGA‑CCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAGGTGAATT  >  AP012306/3527219‑3527516

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑