Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123063,645,6420AC22.5% 909.8 / 34.8 347T318P (ACC→CCC) aceBmalate synthase A
Reads supporting (aligned to +/- strand):  ref base A (197/72);  new base C (0/78);  total (197/150)
Fisher's exact test for biased strand distribution p-value = 7.92e-36
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 389 total aligned reads displayed.

ATATTGTTGGTCTGAACTGCGGTCGTTGGGATTACATCTTCAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTG‑TTG‑ATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATCGCTGGAAGCCAATAACGGTCACGATGGCACATGGATCGC  >  AP012306/3645493‑3645790
                                                                                                                                                       |                                                                                                                                                    
atatTGTTGGTCTGAACTGCGGTCGTTGGGATTACATCTTCAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTG‑TTG‑ATTaaaa                                                                                                                                                      >  1:87625/1‑150 (MQ=255)
  atTGTTGGTCTGAACTGCGGTCGTTGGGATTACATCTTCAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTG‑TTG‑ATTAAAAcc                                                                                                                                                    <  2:3586440/150‑1 (MQ=255)
   ttgttgGTCTGAACTGCGGTCGTTGGGATTACATCTTCAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTG‑TTG‑ATTAAAACCt                                                                                                                                                   <  2:3198088/150‑1 (MQ=255)
      ttgGTCTGAACTGCGGTCGTTGGGATTACATCTTCAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTG‑TTG‑ATTAAAACCTGcc                                                                                                                                                <  2:2627716/150‑1 (MQ=255)
       tgGTCTGAACTGCGGTCGTTGGGATTACATCTTCAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTG‑TTG‑ATTAAAACCTGCCa                                                                                                                                               >  2:1244556/1‑150 (MQ=255)
        ggTCTGAACTGCGGTCGTTGGGATTACATCTTCAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTG‑TTG‑ATTAAAACCTGCCAt                                                                                                                                              >  1:852173/1‑150 (MQ=255)
        ggTCTGAACTGCGGTCGTTGGGATTACATCTTCAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTG‑TTG‑ATTAAAACCTGCCAt                                                                                                                                              <  2:3839361/150‑1 (MQ=255)
         gTCTGAACTGCGGTCGTTGGGATTACATCTTCAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTG‑TTG‑ATTAAAACCTGCCATa                                                                                                                                             <  1:1804964/150‑1 (MQ=255)
               aCTGCGGTCGTTGGGATTACATCTTCAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTG‑TTG‑ATTAAAACCTGCCATAAAcgcg                                                                                                                                       >  1:1105851/1‑150 (MQ=255)
                  gCGGTCGTTGGGATTACATCTTCAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTG‑TTG‑ATTAAAACCTGCCATAAACGCGGTg                                                                                                                                    >  1:3465447/1‑150 (MQ=255)
                   cGGTCGTTGGGATTACATCTTCAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTG‑TTG‑ATTAAAACCTGCCATAAACGCGGTGc                                                                                                                                   >  1:1886054/1‑150 (MQ=255)
                     gTCGTTGGGATTACATCTTCAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTG‑TTG‑ATTAAAACCTGCCATAAACGCGGTGCtt                                                                                                                                 >  1:4272496/1‑150 (MQ=255)
                     gTCGTGGGTTTTACATCTCCAGTTATACAAAAACGTGAAAAACCTACCCCGATCGCGTCCTCCCAGACAGACGGGCAGTGAGGAGGGATAACCCATTCCTGAATGTTTACCCCCCCCTG‑TTA‑TTTAAAACCTGCCATAAACGCGGTGCtt                                                                                                                                 <  2:2097308/150‑1 (MQ=255)
                       cGTTGGGATTACATCTTCAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTG‑TTG‑ATTAAAACCTGCCATAAACGCGGTGCtttt                                                                                                                               >  1:3880205/1‑150 (MQ=255)
                         ttGGGATTACATCTTCAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTG‑TTG‑ATTAAAACCTGCCATAAACGCGGTGCTTTTGc                                                                                                                             >  1:1253354/1‑150 (MQ=255)
                             gATTACATCTTCAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTG‑TTG‑ATTAAACCCTGCCATAAACGCGGTGCTTTTGCGATg                                                                                                                         <  1:2980876/150‑1 (MQ=255)
                             gATTACATCTTCAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTG‑TTG‑ATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATg                                                                                                                         >  1:414676/1‑150 (MQ=255)
                               ttACATTTTCATCAACAGCAAAACGTTGAAAACCTATCCCGTTCGGGTCGCCCGATACAGCGAGGTAGTGAGGGAGGAGAAACCGTTCC‑GAAAGATTA‑TTTCGCCCGTTTGTATTAAACCCTGCCATAAACGCGGTGCTTTTGCGATggg                                                                                                                       <  2:1893495/150‑1 (MQ=255)
                                tACATCTTCAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTG‑TTG‑ATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGggc                                                                                                                      >  2:2996439/1‑150 (MQ=255)
                                  cATCTTCAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTG‑TTG‑ATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGggcgg                                                                                                                    >  1:2214346/1‑150 (MQ=255)
                                  cATCTTCAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTG‑TTG‑ATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGggcgg                                                                                                                    >  2:3489896/1‑150 (MQ=255)
                                  cATCTTCAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTG‑TTG‑ATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGggcgg                                                                                                                    >  1:1173674/1‑150 (MQ=255)
                                   aTCTTCAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCCCGCCTG‑TTG‑ATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGggcggc                                                                                                                   <  1:3930162/150‑1 (MQ=255)
                                    tCTTCAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTG‑TTG‑ATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCa                                                                                                                  >  2:3340250/1‑150 (MQ=255)
                                      ttCAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTG‑TTG‑ATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATg                                                                                                                >  1:410699/1‑150 (MQ=255)
                                      ttCAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTG‑TTG‑ATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATg                                                                                                                >  2:39711/1‑150 (MQ=255)
                                       tCAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTG‑TTG‑ATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATgg                                                                                                               >  1:3051800/1‑150 (MQ=255)
                                        cAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTG‑TTG‑ATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATggc                                                                                                              >  1:2883812/1‑150 (MQ=255)
                                        cAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTG‑TTG‑ATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATggc                                                                                                              >  2:580946/1‑150 (MQ=255)
                                        cAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTG‑TTG‑ATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATggc                                                                                                              >  1:3230386/1‑150 (MQ=255)
                                          gCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTG‑TTG‑ATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATggcgg                                                                                                            >  2:802384/1‑150 (MQ=255)
                                            tataTCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTG‑TTG‑ATTAAACCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGgcggcg                                                                                                          <  1:2844659/150‑1 (MQ=255)
                                            tataTCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTG‑TTG‑ATTAAAACCTGCCATAAACGCAGTGCTTTTGCGATGGGCGGCATGgcggcg                                                                                                          >  2:2411193/1‑150 (MQ=255)
                                              taacAAAACGTTGAAAACCTACCCCGATCGCTTCCTGCCAGACAGACGGGCAGGGAGGAGGAAAAACCCATCCCTGATTGTTTCCTCCCCCTTG‑TTG‑ATTAAACCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGtt                                                                                                        <  2:1039812/147‑1 (MQ=255)
                                                tCAAAACGTTAAAAAACTATCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAACCCATTCCTGAATGCTTCCTCCCGCCTG‑TTG‑ATAAAACCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTa                                                                                                      <  2:1588653/150‑1 (MQ=255)
                                                     aCGTTGAAAAACTATCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTG‑TTG‑ATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCg                                                                                                 >  1:3285357/1‑150 (MQ=255)
                                                      cGTTGAAAAACTATCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTG‑TTG‑ATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGa                                                                                                <  1:3619284/150‑1 (MQ=255)
                                                      cGTTGAAAAACTATCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTG‑TTG‑ATAAAACCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGa                                                                                                <  1:1350555/150‑1 (MQ=255)
                                                      cGTTGAAAAACTACCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAACCCATTCCTGAATGCTTACTCCCGCCTG‑TTG‑ATTAAACCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGa                                                                                                <  2:927999/150‑1 (MQ=255)
                                                       tttaaaaaaCTACCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAACCCATCCCTGAATGCTTACTCACGCCTG‑TTG‑ATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAg                                                                                               <  2:1961077/146‑1 (MQ=255)
                                                       gTTGAAAAACTATCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTG‑TTG‑ATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAg                                                                                               >  2:2162911/1‑150 (MQ=255)
                                                        ttGAAAAACTATCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTG‑TTG‑ATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGc                                                                                              <  1:671891/150‑1 (MQ=255)
                                                         tGAAAAACTATCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTG‑TTG‑ATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCa                                                                                             >  2:4114439/1‑150 (MQ=255)
                                                          gAAAAACTATCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTG‑TTG‑ATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCaa                                                                                            >  1:3962088/1‑150 (MQ=255)
                                                               aCTATCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTG‑TTG‑ATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATg                                                                                       <  1:2924463/150‑1 (MQ=255)
                                                                    cccGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTG‑TTG‑ATTAAACCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAagag                                                                                  <  2:456036/150‑1 (MQ=255)
                                                                     ccGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTG‑TTG‑ATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGc                                                                                 >  1:2668848/1‑150 (MQ=255)
                                                                       gaccgcgTCCTGCCAGACAGACAGGCAGTGACGATGGAAAAACCATCCCTGAATGCTTCCTCACGCCTG‑TTG‑ATAAAACCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGcac                                                                               <  2:1529488/147‑1 (MQ=255)
                                                                       gATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTG‑TTG‑ATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGcac                                                                               >  1:4422081/1‑150 (MQ=255)
                                                                       gATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTG‑TTG‑ATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGcac                                                                               >  1:4326581/1‑150 (MQ=255)
                                                                        aTCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTG‑TTG‑ATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGcaca                                                                              >  2:704303/1‑150 (MQ=255)
                                                                          cgcgTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTG‑TTG‑ATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAAt                                                                            >  2:161563/1‑150 (MQ=255)
                                                                            cgTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTG‑TTG‑ATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATaa                                                                          <  1:3761295/150‑1 (MQ=255)
                                                                             gTCCTGCCAGACAGACAGGCAGTGACGATGGATAACCCATTCCTGAATGCTTACTCGCGCCTG‑TTG‑ATTAAACCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAAc                                                                         <  1:3152198/150‑1 (MQ=255)
                                                                             gTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTG‑TTG‑ATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAAc                                                                         >  2:1109268/1‑150 (MQ=255)
                                                                             gTCCTCCCACACAGACAGCAGGTGACGATGGATAACCCATTCAGGAATCTTTCCTCGCGCCGG‑TTA‑ATAAAACCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAAc                                                                         <  2:2808083/150‑1 (MQ=255)
                                                                                  gCCAGACAGACAGGCAGAGACGATGGATAACCCATTCCTAAATGTTTACTCACGCCTG‑TTG‑ATAAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGt                                                                    <  2:4274071/150‑1 (MQ=255)
                                                                                  gCCAAACAGACAGGCAGTGACGATGGATAAACCATTCCTAATTGCTTCCTCACGCTTG‑TTG‑ATTAAAGCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGt                                                                    <  2:2924031/150‑1 (MQ=255)
                                                                                   ccAGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTG‑TTG‑ATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTg                                                                   >  1:1448537/1‑150 (MQ=255)
                                                                                      gacagacaGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTG‑TTG‑ATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTc                                                                >  2:4352194/1‑150 (MQ=255)
                                                                                         agacagGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTG‑TTG‑ATTAAACCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTcaac                                                             <  1:519056/150‑1 (MQ=255)
                                                                                           acagGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTG‑TTG‑ATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTcaacaa                                                           >  2:2593402/1‑150 (MQ=255)
                                                                                            cagGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTG‑TTG‑ATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACaaa                                                          >  2:1471424/1‑150 (MQ=255)
                                                                                            cagGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTG‑TTG‑ATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACaaa                                                          >  2:1803973/1‑150 (MQ=255)
                                                                                             agGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTG‑TTG‑ATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAg                                                         <  1:2518638/150‑1 (MQ=255)
                                                                                              ggCAGTGACGATGAATAATCCATTCCTGAAGGCTTACTCACGCCTG‑TTA‑ATAAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGt                                                        <  1:4016862/150‑1 (MQ=255)
                                                                                               gaaGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTG‑TTG‑ATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTa                                                       <  1:2461231/148‑1 (MQ=255)
                                                                                               gCAGTGACGAGGGAAAACCCATTCCGGAATGCTTCCTCGCGCCTG‑TTG‑ATAAAACCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTa                                                       <  2:4029736/150‑1 (MQ=255)
                                                                                                cAGTGACGATGGATAAACCATTCCTGAATGCTTCCTCACGCCTG‑TTG‑ATTAAACCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTaa                                                      <  2:307594/150‑1 (MQ=255)
                                                                                                   ggACGATGGATAACCCATGCATGAATGCTAACGCCCGCTTG‑TTG‑ATAAAACCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAg                                                   <  2:4395541/149‑1 (MQ=255)
                                                                                                    gACGATGGATAAACCATTCCTGAATGCTTACTCACGCTTG‑TTG‑ATAAAACCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGc                                                  <  1:2031199/150‑1 (MQ=255)
                                                                                                            aTAACCCATTCCTGAATGCTTACTCACGCCTG‑TTG‑ATTAAACCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCATAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAAt                                          <  2:613316/150‑1 (MQ=255)
                                                                                                               aaCCATTCCTGAATGCTTACTCACGCCTG‑TTG‑ATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATCGc                                       >  2:2376605/1‑150 (MQ=255)
                                                                                                               aaCCATTCCTGAATGCTTACTCACGCCTG‑TTG‑ATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATCGc                                       >  1:2240474/1‑150 (MQ=255)
                                                                                                                  cATTCCTGAATGCTTACTCACGCCTG‑TTG‑ATTAAACCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATCGCTgg                                    <  1:2790501/150‑1 (MQ=255)
                                                                                                                  cATTCCTGAATGCTTACTCACGCCTG‑TTG‑ATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATCGCTgg                                    >  1:1579735/1‑150 (MQ=255)
                                                                                                                  cATCCCTGAATGCTTCCTCACGCCTG‑TTG‑ATTAAACCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATCGCTgg                                    <  2:3628808/150‑1 (MQ=255)
                                                                                                                      ccTGAATGCTTACTCACGCCTG‑TTG‑ATTAAACCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATCGCTGGAAGc                                <  2:4297433/150‑1 (MQ=255)
                                                                                                                      ccTGAATGCTTACTCACGCCTG‑TTG‑ATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATCGCTGGAAGc                                >  1:912597/1‑150 (MQ=255)
                                                                                                                       cTGAATGCTTACTCACGCCTG‑TTG‑ATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATCGCTGGAAGcc                               >  2:2660851/1‑150 (MQ=255)
                                                                                                                         gAATGCTTACTCACGCCTG‑TTG‑ATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATCGCTGGAAGCCaa                             >  1:2298035/1‑150 (MQ=255)
                                                                                                                         gAAGGCTTCCTCCCGCCTG‑TTG‑ATTAAACCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATCGCTGGAAGCCaa                             <  1:1503297/150‑1 (MQ=255)
                                                                                                                              cTTACTCACGCCTG‑TTG‑ATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATCGCTGGAAGCCAATAACg                        >  1:776257/1‑150 (MQ=255)
                                                                                                                               ttACTCACGCCTG‑TTG‑ATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATCGCTGGAAGCCAATAACgg                       >  2:3664479/1‑150 (MQ=255)
                                                                                                                               ttACTCACGCCTG‑TTG‑ATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATCGCTGGAAGCCAATAACgg                       >  1:3085725/1‑150 (MQ=255)
                                                                                                                                tACTCACGCCTG‑TTG‑ATTAAACCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATCGCTGGAAGCCAATAACGGt                      <  1:2388752/150‑1 (MQ=255)
                                                                                                                                 aCTCACGCCTG‑TTG‑ATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATCGCTGGAAGCCAATAACGGTc                     >  1:1989271/1‑150 (MQ=255)
                                                                                                                                   tCACGCCTG‑TTG‑ATAAAACCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATCGCTGGAAGCCAATAACGGTCAc                   <  2:4070675/150‑1 (MQ=255)
                                                                                                                                    cACGCCAG‑TTG‑ATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATCGCTGGAAGCCAATAACGGTCACg                  >  2:37049/1‑150 (MQ=255)
                                                                                                                                      cccttG‑TTG‑ATTAAACCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATCGCTGGAAGCCAATAACGGTCACGAt                <  2:1335241/146‑1 (MQ=255)
                                                                                                                                       gCCTG‑TTG‑ATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATCGCTGGAAGCCAATAACGGTCACGATg               >  2:2247625/1‑150 (MQ=255)
                                                                                                                                        ccTG‑TTG‑ATTAAACCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATCGCTGGAAGCCAATAACGGTCACGATgg              <  1:3398482/150‑1 (MQ=255)
                                                                                                                                           g‑TTG‑ATAAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATCGCTGGAAGCCAATAACGGTCACGATGGcac           <  1:100298/150‑1 (MQ=255)
                                                                                                                                             ttG‑ATTAAACCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATCGCTGGAAGCCAATAACGGTCACGATGGcaca          <  1:3676048/150‑1 (MQ=255)
                                                                                                                                             ttG‑ATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATCGCTGGAAGCCAATAACGGTCACGATGGcaca          >  1:3545768/1‑150 (MQ=255)
                                                                                                                                              tG‑ATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATCGCTGGAAGCCAATAACGGTCACGATGGCACAt         >  1:2073299/1‑150 (MQ=255)
                                                                                                                                                 aTTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATCGCTGGAAGCCAATAACGGTCACGATGGCACATgg       >  1:574310/1‑150 (MQ=255)
                                                                                                                                                    aaacCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATCGCTGGAAGCCAATAACGGTCACGATGGCACATGGATc    <  1:439953/150‑1 (MQ=255)
                                                                                                                                                    aaaaCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATCGCTGGAAGCCAATAACGGTCACGATGGCACATGGATc    >  1:1849960/1‑150 (MQ=255)
                                                                                                                                                      aaCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATCGCTGGAAGCCAATAACGGTCACGATGGCACATGGATCGc  >  2:2025610/1‑150 (MQ=255)
                                                                                                                                                       |                                                                                                                                                    
ATATTGTTGGTCTGAACTGCGGTCGTTGGGATTACATCTTCAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTG‑TTG‑ATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATCGCTGGAAGCCAATAACGGTCACGATGGCACATGGATCGC  >  AP012306/3645493‑3645790

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑