Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123063,903,7910AG23.6% 586.5 / 54.7 242E189G (GAA→GGA) yjgJpredicted transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (32/152);  new base G (57/0);  total (90/152)
Fisher's exact test for biased strand distribution p-value = 1.43e-32
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 349 total aligned reads displayed.

GACCATTTATGACTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTG‑ATGCGGGCCTTGA‑CCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGC‑GTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAACGTT  >  AP012306/3903642‑3903940
                                                                                                                                                     |                                                                                                                                                         
gACCATTTATGACTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGa                                                                                                                                                           <  2:335012/150‑1 (MQ=255)
   cATTTATGACTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGc                                                                                                                                                        <  1:2064323/150‑1 (MQ=255)
       tATGACTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATa                                                                                                                                                    >  1:3290957/1‑150 (MQ=255)
        aTGACTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATaa                                                                                                                                                   <  2:2998673/150‑1 (MQ=255)
            cTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAAc                                                                                                                                               >  1:2620876/1‑150 (MQ=255)
            cTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAAc                                                                                                                                               <  2:2713069/150‑1 (MQ=255)
             tACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACt                                                                                                                                              <  2:93544/150‑1 (MQ=255)
               cATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAACTAt                                                                                                                                            >  1:4146530/1‑150 (MQ=255)
               cATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTAt                                                                                                                                            <  2:890696/150‑1 (MQ=255)
                aTCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAGCCGCTAAAAACTAtt                                                                                                                                           >  2:3632635/1‑150 (MQ=255)
                 tCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTc                                                                                                                                          <  1:1029497/150‑1 (MQ=255)
                  cGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAACTATTCt                                                                                                                                         >  2:2083898/1‑150 (MQ=255)
                   gCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAACTATtttt                                                                                                                                        >  1:3450798/1‑150 (MQ=255)
                     cAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAATTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTga                                                                                                                                      <  1:3485797/150‑1 (MQ=255)
                      aGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGGGATAAAAAATATTTTTgag                                                                                                                                     >  2:1475037/1‑150 (MQ=255)
                       ggcggcATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGGTAAAAACTATTCTTtagg                                                                                                                                    >  1:3211904/1‑150 (MQ=255)
                        gcggcATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGGGTGATTAAAACCATTTTTgcgga                                                                                                                                   >  1:1164128/1‑150 (MQ=255)
                        gcggcATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAGGCGATAAAAAATATttttttggg                                                                                                                                   >  2:2574932/1‑145 (MQ=255)
                          ggcATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGt                                                                                                                                 <  1:1597019/150‑1 (MQ=255)
                           gcATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAACTATTCTTGAGGAGTg                                                                                                                                >  1:1719338/1‑150 (MQ=255)
                           gcATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTg                                                                                                                                <  1:1917490/150‑1 (MQ=255)
                            cATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAAATATTTTTTAGGAGTG‑a                                                                                                                              >  2:2687355/1‑150 (MQ=255)
                            cATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTG‑a                                                                                                                              <  2:2884568/150‑1 (MQ=255)
                              tCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTG‑ATg                                                                                                                            <  1:2982073/150‑1 (MQ=255)
                                cacaACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGTGGAAGCGAGAAAAACTATTATTTAGGAGGG‑ATGCg                                                                                                                          >  2:4132862/1‑150 (MQ=255)
                                       cgcACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAACTATTCTTTAGGAGGG‑ATTCGGGCCttt                                                                                                                   >  1:2773151/1‑149 (MQ=255)
                                        gcACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGGGATAAAAACTATTCTTGAGGAGGG‑ATGCCGGCCTTGa                                                                                                                  >  1:2420846/1‑150 (MQ=255)
                                        gcACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTG‑ATGCGGGCCTTGa                                                                                                                  >  1:559711/1‑150 (MQ=255)
                                              tgtgtgACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAAAAATTTTTGAGGAGGGAATGGGGGCCTGAA‑cccaa                                                                                                            >  2:1079654/1‑148 (MQ=255)
                                               gtgtgACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGGTAAAAAATATTCTTGAGGAGGG‑GTGCGGGGCTTGA‑CCCG‑aaa                                                                                                         >  2:4091084/1‑150 (MQ=255)
                                                 gtgACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTG‑ATGCGGGCCTTGA‑CCCG‑AAAgg                                                                                                       <  2:842720/150‑1 (MQ=255)
                                                    aCTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCGCGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCTATGGCGGGGGGAGGGATAAAAAATATTTTTGTGGGGGG‑ATGCGGGCCTTGA‑CCCC‑AAAAGCgg                                                                                                    >  2:3400910/1‑150 (MQ=255)
                                                    aCTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTG‑ATGCGGGCCTTGA‑CCCG‑AAAGGCgg                                                                                                    <  1:1237240/150‑1 (MQ=255)
                                                       gATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAAATATTCTTGAGGAGGG‑ATGCGGGGCTTGA‑CCCG‑AAAGGAGGGaa                                                                                                 >  2:1608385/1‑150 (MQ=255)
                                                        aTTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGGGGTAAAAAATATTTTTTTGGGGGG‑ATGGGGGGCTTGACCCCC‑AAAGGGGGGaa                                                                                                 >  2:1048949/1‑150 (MQ=255)
                                                           ttATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGGGCGATAAAAAATATTCTTTAGGAGGG‑ATGCGGGCCTTGA‑CCCG‑AAAAGCGGGGAAgag                                                                                             >  2:2397520/1‑148 (MQ=255)
                                                           ttATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGGTAAAAACTATTCTTTAGGAGTG‑ATGCGGGCCTTTACCCCA‑AAAGGGGGGAAAgg                                                                                              >  2:682153/1‑149 (MQ=255)
                                                           ttATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAACTATTTTTGAGGGGTG‑ATGCGGGGCTTGA‑CCCG‑AAAGGGGGGAAAgcc                                                                                             >  2:3653464/1‑149 (MQ=255)
                                                           ttATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAACTATTCTTTAGGAGTG‑ATGCGGGCCCTGA‑CCCG‑AAAGGCGGGGAAgcg                                                                                             >  1:508374/1‑150 (MQ=255)
                                                            tATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAACTATTCTTGAGGAGTG‑ATGCGGGCCTTGA‑CCCG‑AAAAGCGGGGAAgcgc                                                                                            >  1:3826053/1‑150 (MQ=255)
                                                            tATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTG‑ATGCGGGCCTTGA‑CCCG‑AAAGGCGGGGAAgcgc                                                                                            >  1:1836979/1‑150 (MQ=255)
                                                             aTGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTG‑ATGCGGGCCTTGA‑CCCG‑AAAGGCGGGAAAGCGcc                                                                                           <  2:1754392/150‑1 (MQ=255)
                                                              tGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTG‑ATGCGGGCCTTGA‑CCCG‑AAAGGCGGGAAAGCGCCt                                                                                          <  2:1172269/150‑1 (MQ=255)
                                                               gAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGGTAAAAAATATTCTTTAGGAGGG‑ATGCGGGCCTTGA‑CCCG‑AAAGGCGGGGAAGCGCCTg                                                                                         >  1:3570851/1‑150 (MQ=255)
                                                                aGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGGGATAAAAAATATTTTTGTGGAGGG‑ATGCGGGCCTTTG‑CCCG‑CAAGGCGGGGAAGCGcccgg                                                                                        >  2:3483754/1‑147 (MQ=255)
                                                                 gTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTG‑ATGCGGGCCTTGA‑CCCG‑AAAGGCGGGAAAGCGCCTGcc                                                                                       <  1:646940/150‑1 (MQ=255)
                                                                   aCCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGAAAAAAAATATTTTTTAGGAGGG‑GGGGGGGGCCTGA‑ACCGAAAAGGGGGGAAAG‑GCccgccgg                                                                                     >  2:3198345/1‑149 (MQ=255)
                                                                         aTGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGGGATAAAAAATATTCTTGAGGGGTG‑ATGCGGGGCTTGA‑CCCG‑AAAAGCGGGAAAAAGCCTGCCGGTAAAtt                                                                               >  2:2463204/1‑150 (MQ=255)
                                                                         aTGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAACTATTCTTGAGGAGTG‑ATGCGGGCCTTTA‑CCCG‑AAAAGCGGGGAAACGCCCGCCGATAAGtt                                                                               >  1:2810933/1‑150 (MQ=255)
                                                                           gTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAAATATTCTTTTGGGGGG‑ATGGGGGCCTTGA‑CCCC‑AAAGGCGGGAAAACGCCCGCCCAGAAATtgt                                                                             >  2:1264596/1‑150 (MQ=255)
                                                                            tCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACACCTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTG‑ATGCGGGCCTTGA‑CCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTtgtg                                                                            <  2:398415/150‑1 (MQ=255)
                                                                             cTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGGTAAAAAATATTTTTTTGGGGTG‑ATGCGGGCCCTTG‑CCCC‑AAAGGCGGGGAAGCGCCTGCCGGTAAGtttggg                                                                           >  2:3734096/1‑146 (MQ=255)
                                                                             cTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAACTATTTTTTAGGGGTG‑ATGCGGGCCTTGA‑CCCG‑AAAGGCGGGGAAACGCCCGCCGGTAAGTTGTGa                                                                           >  2:168066/1‑150 (MQ=255)
                                                                              tGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAACTATTCTTGAGGAGGG‑ATGCGGGGCTTGA‑CCCG‑AAAGGCGGGGAAACGCCCGCCGGTAAATTGTGAt                                                                          >  2:452893/1‑150 (MQ=255)
                                                                               gggCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAAATATTCTTGAGGAGTG‑ATGCGGGCCTTGA‑CCCG‑AAAGGCGGGAAAGCGCCCGCCGATAAGTTGTGATa                                                                         >  1:1491470/1‑150 (MQ=255)
                                                                                 gCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTG‑ATGCGGGCCTTGA‑CCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAg                                                                       <  1:3075344/150‑1 (MQ=255)
                                                                                 gCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTG‑ATGCGGGCCTTGA‑CCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAg                                                                       <  1:2679251/150‑1 (MQ=255)
                                                                                  cTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTG‑ATGCGGGCCTTGA‑CCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGa                                                                      <  1:1935049/150‑1 (MQ=255)
                                                                                  cTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTG‑ATGCGGGCCTTGA‑CCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGa                                                                      <  2:3140707/150‑1 (MQ=255)
                                                                                  cTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTG‑ATGCGGGCCTTGA‑CCCG‑AAAAGCGGGGAAGCGCCCGCCCATAAGTTGTGATAAGa                                                                      >  1:814703/1‑150 (MQ=255)
                                                                                   tttCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGGTAAAAACTATTCTTGAGGAGTG‑ATGCGGGCCTTGA‑CCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGGTGTGATAAGAc                                                                     >  1:1557915/1‑150 (MQ=255)
                                                                                      cTGCAAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTG‑ATGCGGGCCTTGA‑CCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACaat                                                                  <  2:1917557/150‑1 (MQ=255)
                                                                                          gAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTG‑ATGCGGGCCTTGA‑CCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAAtt                                                              <  1:3776200/150‑1 (MQ=255)
                                                                                               cACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAACTATTCTTGAGGAGTG‑ATGCGGGCCTTGA‑CCCG‑AAAGGCGGGGAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCAcgc                                                         >  1:2864881/1‑150 (MQ=255)
                                                                                                     agGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTG‑ATGCGGGCCTTGA‑CCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGC‑GTTAAg                                                  <  2:2823652/150‑1 (MQ=255)
                                                                                                       gggCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTG‑ATGCGGGCCTTGA‑CCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGC‑GTTAAGGc                                                <  2:716063/150‑1 (MQ=255)
                                                                                                         gCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTG‑ATGCGGGCCTTGA‑CCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGC‑GTTAAGGCTa                                              <  1:963439/150‑1 (MQ=255)
                                                                                                          cACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAAATATTTTTTAGGGGGG‑ATGCGGGGCTTGA‑CCCG‑AAAAGCGGGGAAAAGCCCGCCGATAAGTTGTGGTAAAACAAAAAATAACCC‑GTTAAGGGTAg                                             >  2:4155362/1‑150 (MQ=255)
                                                                                                           aCTCTATAGAACACCTCTGTCCAACAGCTGCAATGGGGGGGGAAGGGATAAAAACTATTCTTGAGGAGTG‑ATGCGGGCTTTGA‑CCCG‑AAAGGCGGGAAAGCCCCTGCCGATAAGTTGTGATAAGACAATAATTCACGC‑GTTAAGGCTAGc                                            <  1:3675918/150‑1 (MQ=255)
                                                                                                           aCTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTG‑ATGCGGGCCTTGA‑CCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGC‑GTTAAGGCTAGc                                            <  1:2908669/150‑1 (MQ=255)
                                                                                                            ctctATAGAACACTGCTGTGAAACAGGTCCAAGGGCGGGGGAAGAGAAAAAAACTATTCTTGAGGAGTG‑ATGGGGGCTTTGA‑CCGG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGC‑GTTAAGGCTAGCg                                           <  2:2309736/150‑1 (MQ=255)
                                                                                                            ctctATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAAAAATTTTTTGGGGGGG‑ATGCGGGCCTTGC‑CCCG‑AAAGGGGGGGAAACGCCTGCCGGGAAAGTGTGATAAGACAATAAATTTAGC‑GTTAAAGCTAGCg                                           >  2:1813245/1‑150 (MQ=255)
                                                                                                             tctATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTG‑ATGCGGGCCTTGA‑CCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGC‑GTTAAGGCTAGCgg                                          <  2:1409000/150‑1 (MQ=255)
                                                                                                             tctATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTG‑ATGCGGGCCTTGA‑CCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGC‑GTTAAGGCTAGCgg                                          <  2:1443026/150‑1 (MQ=255)
                                                                                                                     acaacTCTGTGCAACAGCTGCAATGGCGGGGGGAGGGATAAAAAAAATTTTTTAGGAGTG‑GTGCGGGCCTTTA‑CCCC‑AAAGGCGGGGAAGCGGCCCCCGGTAAGGTGTGGTAAGAAAAAAATTTACGC‑GGTAAGGGGAGCGGGAATGAtt                                  >  2:135501/1‑150 (MQ=255)
                                                                                                                      caacTCTGGGCAACAGCTGCAATGGGGGGGGAAGCGATAAAAACTATTCTTGAGGAGTG‑ATGCGGGCCTTGA‑CCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGC‑GTTAAGGCTAGCGGAATTGATTa                                 <  1:2131734/150‑1 (MQ=255)
                                                                                                                       aacTCTGTGCAACAGCTGCAAGGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTG‑ATGCGGGCCTTGA‑CCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGC‑GTTAAGGCTAGCGGAATTGATTAt                                <  2:3099392/150‑1 (MQ=255)
                                                                                                                          tctGTGCAACAGCTGAAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTG‑ATGCGGGCTTTGA‑CCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGC‑GTTAAGGCTAGCGGAATTGATTATCtt                             <  2:2161735/150‑1 (MQ=255)
                                                                                                                             gtgCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTG‑ATGCGGGCCTTGA‑CCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGC‑GTTAAGGCTAGCGGAATTGATTATCTTTTc                          <  1:3364240/150‑1 (MQ=255)
                                                                                                                             gtgCAACAGCTGCAATGGCGGGGGAAGCGATAAAAAATATTTTTGAGGGGTG‑ATGCGGGGCTTGA‑CCCG‑AAAAGCGGGGAAGCGCCTGCCGGTAAGTTGTGATAAAAAAAAAAATCACGC‑GTTTAGGGTAGCGGAAATGATTATCttttt                          >  2:1376107/1‑149 (MQ=255)
                                                                                                                              tgCAACAGCTGCAATGGCGGGGGGAGCGATAAAAAATATTTTTTTGGAGGG‑ATGCGGGCCTTGA‑CCCG‑AAAGGCGGGGAAACGCCCGCCGGTAAATTGTGGTAAAACAAAAAATTACGCGGTTAAGG‑TAGCGGGATTGATTTTCtttttg                         >  2:4295318/1‑148 (MQ=255)
                                                                                                                                cAACAGCTGCAATGGCGGGGGGAGCGGTAAAAAATATTTTTTTGGAGGG‑ATGCGGGGCTTGA‑CCCG‑GAAGGGGGGGAAGGGCCTGCCGGTAAGTTGGGGTAAGACAATAATTCACGC‑GTTAAGGCTAGCGGAATTGGTTTTCTTTTTGtt                       >  2:653245/1‑149 (MQ=255)
                                                                                                                                cAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTG‑ATGGGGGCCTTGA‑CCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGC‑GTTAAGGCTAGCGGAATTGATTATCTTTTCGta                       <  2:2619864/150‑1 (MQ=255)
                                                                                                                                cAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTGGAGGAGTG‑ATGCGGGCCTTGA‑CCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGC‑GTTAAGGCTAGCGGAATTGATTATCTTTTCGta                       <  1:3953148/150‑1 (MQ=255)
                                                                                                                                 aaCAGCTGCAATGGCGGGGGGAGGGTTAAAAACAATTTTTTAGGCGGG‑GTGGGGGGCTTTA‑CCCG‑AAAAGCGGGGAAAAACCTGCCGGTAAATTGTGGTTAAAAAATAATTAACCC‑GCTAAGGAAAGCGGAATTTGTTATCTTTTTGtcg                      >  2:1528842/1‑148 (MQ=255)
                                                                                                                                   cAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTG‑ATGCGGGCCTTGA‑CCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGC‑GTTAAGGCTAGCGGAATTGATTATCTTTTCGTATaa                    <  1:132063/150‑1 (MQ=255)
                                                                                                                                   cAGCTGCAAGGGGGGGGGAAGCGATAAAAACTATTCTTGAGGAGTG‑ATGCGGGCCTTGA‑CCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGC‑GTTAAGGCTAGCGGAATTGATTATCTTTTCGTATaa                    <  2:3550617/150‑1 (MQ=255)
                                                                                                                                     gCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTG‑ATGCGGGCCTTGA‑CCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGC‑GTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACg                  <  1:4041665/150‑1 (MQ=255)
                                                                                                                                      cTGCAATTGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTG‑ATGCGGGCCTTGA‑CCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGC‑GTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGa                 >  1:4386989/1‑150 (MQ=255)
                                                                                                                                      cTGCAATGGCGGGGGGAGCGATAAAAAATATTTTTGAGGAGGG‑ATGCGGGCCTTGA‑CCCG‑AAAGGCGGGGAAGCGCCTGCCGATAAGTTGTGATAAGACAAAAATTCACGC‑GGTAAGGGTAGCGGAATTGATTATTTTTTCGTTTAAAGa                 >  2:1235900/1‑150 (MQ=255)
                                                                                                                                       tGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTG‑ATGCGGGCCTTGA‑CCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGC‑GTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGAt                <  1:460031/150‑1 (MQ=255)
                                                                                                                                         cAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTG‑ATGCGGGCCTTGA‑CCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGC‑GTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAg              <  1:2340402/150‑1 (MQ=255)
                                                                                                                                         cAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTG‑ATGCGGGCCTTGA‑CCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGC‑GTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAg              <  2:2233099/150‑1 (MQ=255)
                                                                                                                                           aTGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTG‑ATGCGGGCCTTGA‑CCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGC‑GTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGaa            <  1:3155251/150‑1 (MQ=255)
                                                                                                                                               cGGGGGAAGCGATAAAAACTATTCTTGAGGAGTG‑ATGCGGGCCTTGA‑CCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGC‑GTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGa        <  1:1744222/150‑1 (MQ=255)
                                                                                                                                               cGGGGGAAGCGATAAAAACTATTCTTGAGGAGTG‑ATGCGGGCCTTGA‑CCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGC‑GTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGa        >  2:1893888/1‑150 (MQ=255)
                                                                                                                                                gggggAAGCGATAAAAACTATTCTTGAGGAGTG‑ATGCGGGCCTTGA‑CCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGC‑GTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGaa       <  1:2304176/150‑1 (MQ=255)
                                                                                                                                                 ggggAAGCGATAAAAACTATTCTTGAGGAGTG‑ATGCGGGCCTTGA‑CCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGC‑GTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGaaa      <  1:1426141/150‑1 (MQ=255)
                                                                                                                                                   gaaaGCAATAAAAAATATTCTGGAGGAGTG‑ATGCCGGTCTTAA‑CCCG‑AAAGGGGGAAAAGCACCTGCCGATAAGTTGTGATAAGACAATAATTCACGC‑GTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAACg    <  2:3863768/148‑1 (MQ=255)
                                                                                                                                                     aaGCGATAAAAACTATTCTTGAGGAGTG‑ATGCGGGCCTTGA‑CCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGC‑GTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAACgtt  >  1:271519/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                         
GACCATTTATGACTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTG‑ATGCGGGCCTTGA‑CCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGC‑GTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAACGTT  >  AP012306/3903642‑3903940

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑