Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123063,938,8870CT26.5% 507.1 / 129.0 238S44F (TCT→TTT) bglJDNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (16/159);  new base T (63/0);  total (79/159)
Fisher's exact test for biased strand distribution p-value = 6.78e-43
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 281 total aligned reads displayed.

ACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGAC  >  AP012306/3938738‑3939035
                                                                                                                                                      |                                                                                                                                                     
aCTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATtttttc                                                                                                                                                       <  2:2650304/150‑1 (MQ=255)
  tCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATtttttctt                                                                                                                                                     >  1:2681904/1‑150 (MQ=255)
     tAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CCtttttttttttt                                                                                                                                                  >  2:69315/1‑150 (MQ=255)
      aGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATCTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATtttttctttttc                                                                                                                                                 <  1:1279046/150‑1 (MQ=255)
          aaaTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCTGcc                                                                                                                                             <  1:3447309/150‑1 (MQ=255)
          aaaTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCTGcc                                                                                                                                             <  2:2612570/150‑1 (MQ=255)
           aaTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCAtttttttttttttttcc                                                                                                                                             >  1:888440/1‑145 (MQ=255)
           aaTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCTGCCa                                                                                                                                            <  1:3387554/150‑1 (MQ=255)
                 tCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGG‑CGTTTTTTTTTTTTTTTCCCTGAGaa                                                                                                                                      >  2:1531705/1‑150 (MQ=255)
                     gAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCTGCCATGAGAAGTGa                                                                                                                                  <  1:4051253/150‑1 (MQ=255)
                       gtagtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAgc                                                                                                                                <  1:3642073/150‑1 (MQ=255)
                       gtagtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAgc                                                                                                                                <  1:2393685/150‑1 (MQ=255)
                        tagtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTTCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGAGGT‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAgcg                                                                                                                               <  1:1480853/150‑1 (MQ=255)
                        tagtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑GATTTTTTTTTTTTTTGCCCTGTGaaaggagag                                                                                                                               >  2:585455/1‑143 (MQ=255)
                        tagtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTTTTTTTTTTCCCTTAGGAGTGAgcc                                                                                                                               >  1:68540/1‑149 (MQ=255)
                            aTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CCTTTTTTTTTTTTTTGCCCTGTGAAATGAGGGCagg                                                                                                                           >  2:397139/1‑149 (MQ=255)
                              cGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CCTTTTTTTTTTTTTTTCCATTAGAAGTGAGGGCagagg                                                                                                                         >  1:3577062/1‑149 (MQ=255)
                              cGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTTTTTTTTTGCCATGAGAAGTGAGCGCagaga                                                                                                                         >  2:4058191/1‑150 (MQ=255)
                                gTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATGCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAgg                                                                                                                       <  1:1120938/150‑1 (MQ=255)
                                gTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGG‑CATTTTTTTTTTTTTTTCCCTGGGAAGAGAgcgcggggggg                                                                                                                       >  2:1101747/1‑143 (MQ=255)
                                 tATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTTTTTTTTTTCCCTGAGAAGTGAGCGCAGAGAggg                                                                                                                      >  1:3074292/1‑150 (MQ=255)
                                 tATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTTTTTTTTTGCCCTCCGAAGTGAGCGCCGGGAggg                                                                                                                      >  1:4100248/1‑150 (MQ=255)
                                    tGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGAtt                                                                                                                   <  2:2832645/150‑1 (MQ=255)
                                     gagaGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTa                                                                                                                  <  2:1589980/150‑1 (MQ=255)
                                     gagaGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTa                                                                                                                  <  2:90566/150‑1 (MQ=255)
                                      agagTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTTTTTTTTTTCCATGAGAAGTGAGCGCAGAGAgggggtat                                                                                                                 >  2:430391/1‑145 (MQ=255)
                                         gTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCtt                                                                                                              <  2:811519/150‑1 (MQ=255)
                                          tttATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGG‑CATTTTTTTTTTTTTTTCCCTTAGAAGTGAGCGCGGGGGGGGGGTATCttt                                                                                                             >  2:2400547/1‑149 (MQ=255)
                                           ttATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTTTTTTTTTGCCCTGAGAAGTGAGCGCAGAGAGGGATTATCtttc                                                                                                            >  1:963609/1‑148 (MQ=255)
                                            tATTCAGAAAGTTTGCGGGTACCCCTTATAAGCTCCATCCCTATACCAGTCAGGAGTCATTTCAGGCTGCCATGTCGCGGATCCCGTTTGCCGCGGT‑CCTTTTTTCTTTTTCTGCCATCCGAAGTGAGCGCAGACAGGCATTATCTTccc                                                                                                           <  2:3166884/150‑1 (MQ=255)
                                             aTTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTTCGGCGGT‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCct                                                                                                          <  1:201367/150‑1 (MQ=255)
                                              ttCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCctg                                                                                                         <  2:1458639/150‑1 (MQ=255)
                                               tCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTTTTTTTTTTCCCTGAGAAGTGGGCGGAGGGAGGGGGTATTTTTCctga                                                                                                        >  2:3369472/1‑150 (MQ=255)
                                                 aGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCctgact                                                                                                      <  1:1629743/150‑1 (MQ=255)
                                                   aaaGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTTTGCCCTTAGGAGTGAGAGGAGAGAGGGGTTATCTTGCctcactta                                                                                                    >  1:3614605/1‑148 (MQ=255)
                                                    aaGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTTTTTTTTTTCCCTTTGAAGGGAGCGCAGAGGGGGGTTATCTTGCcttactgta                                                                                                   >  2:2048359/1‑148 (MQ=255)
                                                      gTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CCTTTTTTTTTTTTTTTCCATGTGAAGTGGGGGCAGAGAGGGGGTATTTTGCCTGACTGAACt                                                                                                 >  1:2666032/1‑150 (MQ=255)
                                                      gTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACt                                                                                                 <  1:2153056/150‑1 (MQ=255)
                                                       tttGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CCTTTTTTTTTTTTTTTCCATTAGAAGAGAGCGCGGGGGGGGATTATCTTTCCTGACTGAAATg                                                                                                >  2:2781794/1‑150 (MQ=255)
                                                          gCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCg                                                                                             <  1:1332988/150‑1 (MQ=255)
                                                           cGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTTTTTTTCTTCCCTGAGAAGTGAGCGCAGAGAGGGGTTATCTTGTCTGACTGAACTGGCgg                                                                                            >  1:234569/1‑149 (MQ=255)
                                                           cGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGa                                                                                            <  1:438458/150‑1 (MQ=255)
                                                             ggTAACCCTTATAAGCTCCATACCTATACCTGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTTTTTTTTTGCCCTTAGAAGTGAGCGCAGAGAGGGGTTATCTTGCCTGACTTAACTGGCGagt                                                                                          >  1:3952116/1‑148 (MQ=255)
                                                             ggTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTTTTCCATGAGAAGTGAGCGCAGAGAGGGGGTATCTTTCCTGACTGAACTGGCGA‑tt                                                                                         >  1:1867943/1‑150 (MQ=255)
                                                             ggTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑tt                                                                                         <  2:1776405/150‑1 (MQ=255)
                                                              gTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTTTTTTTTTGCCATGAGGAGGGGGGGCAGAGAGGGGGTATTTTTTCTGACTGAACTGGCGG‑TTa                                                                                        >  2:1834688/1‑150 (MQ=255)
                                                              gTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTa                                                                                        <  1:1975964/150‑1 (MQ=255)
                                                                aaCCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGACCTGGCGA‑TTAAg                                                                                      <  2:3581403/150‑1 (MQ=255)
                                                                 aCCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGt                                                                                     <  2:465710/150‑1 (MQ=255)
                                                                  cccTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGtt                                                                                    <  2:3505014/150‑1 (MQ=255)
                                                                    cTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTc                                                                                  <  1:1695015/150‑1 (MQ=255)
                                                                     ttATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CTTTTTTTTTTTTTCTGTCCTGAGAAGGGGGGGCAGAGAGGGAGTGTCTTTACTGACTGAACCGGGGG‑TTatattttc                                                                                 >  2:1819271/1‑143 (MQ=255)
                                                                     ttATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGtttgc                                                                                 <  1:2465937/150‑3 (MQ=255)
                                                                     ttATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTcc                                                                                 <  1:248339/150‑1 (MQ=255)
                                                                      tataAGCTCGATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCcg                                                                                <  1:3146123/150‑1 (MQ=255)
                                                                       ataAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCcgc                                                                               <  2:2595261/150‑1 (MQ=255)
                                                                         aaGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTTTTTTTTTTCCATGGGAAGTGGGCGCAGGGGGGGGGTATTTTGCCTGACTGAAATTGCGG‑TTAAGTTTTCGCGt                                                                             >  2:1585005/1‑150 (MQ=255)
                                                                                   ccTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGCGtt                                                                   <  1:1246954/150‑1 (MQ=255)
                                                                                       taCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGt                                                               <  2:774311/150‑1 (MQ=255)
                                                                                        aCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGtt                                                              <  1:1885727/150‑1 (MQ=255)
                                                                                         ccAGTCAGGAGTCATTTCAGGAAGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTa                                                             <  2:2924626/150‑1 (MQ=255)
                                                                                            gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGG‑CATTTTTTTTTTTTTTTCCATTAGAAAAGAGCGCCGAGAGGGGTTATTTTGGCTGACTGAAATGGCGA‑TTAAGTTTTCGCCTACCCCGCGGTTAGTttttt                                                          >  2:2302562/1‑149 (MQ=255)
                                                                                              cAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCg                                                        <  1:258055/150‑1 (MQ=255)
                                                                                               aGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCgg                                                       <  1:836049/150‑1 (MQ=255)
                                                                                                 gAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgat                                                     <  2:301862/150‑1 (MQ=255)
                                                                                                   gTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgatga                                                   <  1:3758990/150‑1 (MQ=255)
                                                                                                     cATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGC‑GATTTTTTTTTTTTTTTCCCCGAGAAGAGGGCGGAGAGAGGGGTTATTTTTCGCGGCTGGACAGGGGATTTAAATTTCC‑CGTACCCCGGGCTTTGTTTTTGGGggttatg                                                 >  2:426432/1‑150 (MQ=255)
                                                                                                     cATTTCAGGATACCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTTTTTGTTTTCCCTGAGAAGGGAGGGGCGGGGGGGGTTTTTTTTTCTGACTGAACTGGCGA‑TTAAGTTTTCCCGTACCCCGCGGTTTGTTTTTGTGggtgatg                                                 >  2:3633746/1‑150 (MQ=255)
                                                                                                       tttCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgatgatgat                                               <  2:4184489/150‑1 (MQ=255)
                                                                                                       tttCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgatgatgat                                               <  1:4012416/150‑1 (MQ=255)
                                                                                                           aGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTg                                           <  2:3461319/150‑1 (MQ=255)
                                                                                                           aGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTg                                           <  1:575897/150‑1 (MQ=255)
                                                                                                            ggATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGa                                          <  1:2941540/150‑1 (MQ=255)
                                                                                                              aTGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTTTTTTTTTTTCCTTGGAAGAGAGGGGGGAGAGGGGTTTTTTTTTCCGGCTTGACTGGGGG‑TTAAATTTTTGCGTGCCCGGCGGTTTGTTTTTTTGGGGGATGATAttttag                                        >  2:1763571/1‑146 (MQ=255)
                                                                                                              aTGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAg                                        <  2:4378559/150‑1 (MQ=255)
                                                                                                              aTGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAg                                        <  1:207002/150‑1 (MQ=255)
                                                                                                                  cATGTCGCGTATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCg                                    <  2:1112385/150‑1 (MQ=255)
                                                                                                                   aTGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCgg                                   <  1:3289309/150‑1 (MQ=255)
                                                                                                                     gTCGCGGATCTCGTTTGCGGCGGT‑TATTTTTTTTTTTTTTTCCCTGAGAAGGGAGGGGAGGGAGGGGGTTTTTTTCCTGAGTGAAATTGGGA‑TTAAGTTTTCGCGGACCCGGCGTTTTGTTTTTTTGGGTGATGATATTTAAGGTCgggt                                 >  2:2642978/1‑148 (MQ=255)
                                                                                                                       cgcgGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGa                               <  2:2704492/150‑1 (MQ=255)
                                                                                                                         cgGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGAtt                             <  1:4300693/150‑1 (MQ=255)
                                                                                                                          ggATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTg                            <  1:1038439/150‑1 (MQ=255)
                                                                                                                                cGTTTGCGGCGGT‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCAt                      <  2:517251/150‑1 (MQ=255)
                                                                                                                                 gTTTGCGGCGGT‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCAtt                     <  2:2106191/150‑1 (MQ=255)
                                                                                                                                 gTTTGCGACGGT‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTTCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGGTCAtt                     <  2:2383308/150‑1 (MQ=255)
                                                                                                                                   ttGCGGCGGT‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGt                   <  1:1750962/150‑1 (MQ=255)
                                                                                                                                   ttGCGGCGGT‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGt                   <  1:3216818/150‑1 (MQ=255)
                                                                                                                                   ttGCGGCGGT‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGt                   <  1:1448622/150‑1 (MQ=255)
                                                                                                                                         cggT‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCAt             <  1:2743018/150‑1 (MQ=255)
                                                                                                                                         cggT‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCAt             >  2:3248397/1‑150 (MQ=255)
                                                                                                                                         cggT‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCAt             <  2:3886788/150‑1 (MQ=255)
                                                                                                                                           gT‑CATTTTTTCTGTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCa           >  2:4326280/1‑150 (MQ=255)
                                                                                                                                            t‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCAc          <  2:3703647/150‑1 (MQ=255)
                                                                                                                                               aTTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTTATTGTCGCCATCACCg        <  2:640202/150‑1 (MQ=255)
                                                                                                                                               aTTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCg        <  2:1653313/150‑1 (MQ=255)
                                                                                                                                                ttttttCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGc       >  2:1324522/1‑150 (MQ=255)
                                                                                                                                                  ttttctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTg     >  1:3887057/1‑150 (MQ=255)
                                                                                                                                                  ttttctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTg     >  2:2503307/1‑150 (MQ=255)
                                                                                                                                                     tctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGAc  >  2:4317761/1‑150 (MQ=255)
                                                                                                                                                     tctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGAc  >  2:2175843/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
ACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGAC  >  AP012306/3938738‑3939035

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑