Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP012306935,0540GT30.6% 168.0 / 38.1 72intergenic (+24/‑1406)putP/ycdOproline:sodium symporter/conserved protein
Reads supporting (aligned to +/- strand):  ref base G (14/36);  new base T (22/0);  total (36/36)
Fisher's exact test for biased strand distribution p-value = 4.24e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 142 total aligned reads displayed.

GCAGTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTT‑CAGCAGCGGGTTGCGCT‑CCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTC‑GGGGAACGCTTCAGCGATTTTGACGCCACCAACA  >  AP012306/934906‑935203
                                                                                                                                                      |                                                                                                                                                         
gCAGTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑Ttttgt                                                                                                                                                          <  2:2881255/150‑1 (MQ=255)
 cAGTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑Ttttgtt                                                                                                                                                         <  1:3736806/150‑1 (MQ=255)
  aGTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑Ttttgttt                                                                                                                                                        <  2:2174083/150‑1 (MQ=255)
  aGTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑Ttttgttt                                                                                                                                                        >  1:2436701/1‑150 (MQ=255)
   gTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑Tgttgtttt                                                                                                                                                       >  2:1078446/1‑149 (MQ=255)
     aTTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGCTTTTTTTTgg                                                                                                                                                      >  2:3666999/1‑150 (MQ=255)
      ttGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGG‑TTTTTTTTGGc                                                                                                                                                     >  1:1018009/1‑150 (MQ=255)
      ttGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑gtttgtttcgtt                                                                                                                                                    >  1:3035947/1‑146 (MQ=255)
      ttGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑GGTTATTTTGCt                                                                                                                                                    >  2:492813/1‑150 (MQ=255)
       tGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTGGGtt                                                                                                                                                   >  1:1252679/1‑150 (MQ=255)
       tGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCtt                                                                                                                                                   <  2:1618230/150‑1 (MQ=255)
        gggATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑GTTTTTTTTGGTT‑c                                                                                                                                                 >  2:2372276/1‑150 (MQ=255)
        gggATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑GTTTTTTTTGCTT‑c                                                                                                                                                 >  2:2464663/1‑150 (MQ=255)
         ggATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTTGcgtta                                                                                                                                                 >  1:1856177/1‑146 (MQ=255)
          gATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTGGCTT‑cag                                                                                                                                               >  1:3459820/1‑150 (MQ=255)
          gATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTT‑cag                                                                                                                                               <  1:1986377/150‑1 (MQ=255)
          gATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑GTTTGTTTTGGTT‑cag                                                                                                                                               >  2:381874/1‑150 (MQ=255)
           aTTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGG‑TTTTTTTTGGCtcagc                                                                                                                                                >  1:3095185/1‑146 (MQ=255)
           aTTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTTGCTT‑ccgc                                                                                                                                              >  1:1467393/1‑150 (MQ=255)
           aTTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑GTTTGTTTTGCTT‑tagc                                                                                                                                              >  1:3574675/1‑150 (MQ=255)
            ttGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTT‑cagca                                                                                                                                             <  1:419731/150‑1 (MQ=255)
             tGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑TG‑TTTTGTTTGGCTT‑cagcag                                                                                                                                            >  1:838513/1‑150 (MQ=255)
             tGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGGTT‑ctgcag                                                                                                                                            >  2:2621213/1‑150 (MQ=255)
              gTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTT‑Cagcagc                                                                                                                                           <  1:2179090/150‑1 (MQ=255)
              gTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTT‑Cagcagc                                                                                                                                           <  1:1526812/150‑1 (MQ=255)
               tAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTT‑CAGCAGCg                                                                                                                                          <  2:3399691/150‑1 (MQ=255)
                 gtgtTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGGTT‑TCTCAGCggg                                                                                                                                        >  2:1394078/1‑150 (MQ=255)
                 gtgtTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTTGCTT‑CAGCAGCcgg                                                                                                                                        >  1:1076655/1‑150 (MQ=255)
                  tgtTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTTGCTT‑TCGCAGCgggg                                                                                                                                       >  1:3149728/1‑149 (MQ=255)
                   gtTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGCTT‑CTGACGCggggt                                                                                                                                      >  2:73932/1‑148 (MQ=255)
                    tttAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑CTTTGTTTGGGGT‑GAGCAGCGGGGTg                                                                                                                                     >  2:1228833/1‑150 (MQ=255)
                     ttAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGCTT‑CCGCAGCggggggc                                                                                                                                    >  2:1314697/1‑146 (MQ=255)
                     ttAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGCTT‑CAGCAGCGGGGTgc                                                                                                                                    >  1:1508717/1‑150 (MQ=255)
                       aGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTTGGTT‑CAGCAGCGGGGGgcgc                                                                                                                                  >  2:3948578/1‑150 (MQ=255)
                        gTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGCTT‑TCGCAGCGGGGGGCGCt                                                                                                                                 >  2:769895/1‑150 (MQ=255)
                        gTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑GTTTTTTTGGGCT‑CCGCCGCGGGGTgggcc                                                                                                                                 >  2:510520/1‑149 (MQ=255)
                         tttGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGCGT‑TTGCAGCGGGGTGGGCT‑c                                                                                                                               >  2:2503059/1‑150 (MQ=255)
                         tttGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑GTTTTTTTTGCTTCCAGCGCCGGGTTgccgc                                                                                                                                 >  2:3221866/1‑147 (MQ=255)
                          ttGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTT‑CAGCAGCGGGTTGCGCT‑cc                                                                                                                              <  1:3453755/150‑1 (MQ=255)
                           tGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGGTT‑CCGCAACGGGGTTCGCT‑ccc                                                                                                                             >  1:4023049/1‑150 (MQ=255)
                              tGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGCTT‑TAGCAGCGGGGTGCGCT‑CCCttt                                                                                                                          >  1:635184/1‑149 (MQ=255)
                              tGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGCTT‑CCGCAGCGGGGTGCGCT‑cccctt                                                                                                                          >  2:1628612/1‑147 (MQ=255)
                               gggTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTTGCTT‑CAGCAGCGGGGTGCGCT‑cccctta                                                                                                                         >  1:3754377/1‑146 (MQ=255)
                                ggTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTT‑CAGCAGCGGGTTGCGCT‑CCCTTAAt                                                                                                                        <  1:109313/150‑1 (MQ=255)
                                ggTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTT‑CAGCAGCGGGTTGCGCT‑CCCTTAAt                                                                                                                        <  1:502490/150‑1 (MQ=255)
                                       cgcCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑GTTTTTTTTGCTT‑TTGCCGCGGGGTGGGCC‑CCCCTTATGTTCCTc                                                                                                                 >  2:2217865/1‑150 (MQ=255)
                                        gcCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGGTT‑TCGCCGCGGGGTGGGCC‑CCCCTTATTTGCCCCg                                                                                                                >  2:2247557/1‑150 (MQ=255)
                                         ccGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGCTT‑CAGCAGCGGGGTTCGCT‑CCCTTTATGTGCCTCGc                                                                                                               >  1:280121/1‑150 (MQ=255)
                                           gTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTGTGTT‑CAGCAGCGGGGTTCGCT‑CCCCTTATGTGCCTCGCCa                                                                                                             >  2:1176459/1‑150 (MQ=255)
                                           gTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTT‑TAGCCACCGGGTACGCC‑CCCCTGAAGTGCCCCGcct                                                                                                             >  1:522285/1‑149 (MQ=255)
                                              aGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑GTTTTTTTTGGTT‑CTGCAGCGGGGTGCGCT‑CCCCTTATGTGGCTcccccttt                                                                                                          >  2:1189506/1‑146 (MQ=255)
                                                cggcgATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTT‑CAGCAGCGGGGTGCGCT‑CCCTTTATGTGCCTCGCCCTATaa                                                                                                        >  1:1753506/1‑150 (MQ=255)
                                                cggcgATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑GTTTTTTTTGGTT‑CAGCAGAGGGGTGGGCT‑CCCCTAATGTGGCTCGCCCTATaa                                                                                                        >  2:1448094/1‑150 (MQ=255)
                                                  gcgATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTT‑CAGCAGCGGGTTGCGCT‑CCCTTAATGTGCCTCGCCATATAAAt                                                                                                      <  2:1779301/150‑1 (MQ=255)
                                                    gATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑GTTTGTTTGGCTT‑CAGCAGCGGGGTGGGCT‑CCCCTTAAGTGCCTCGCCCTATAAAATg                                                                                                    >  1:754097/1‑150 (MQ=255)
                                                     ccgatctaaCGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTT‑CAGCAGCGGGTTGCGCT‑CCCTTAATGTGCCTCGCCATATAAATTGa                                                                                                   <  1:2875450/143‑1 (MQ=255)
                                                     aTGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGCTT‑CCGCAGCGGGGTGCGCT‑CCCTTTATATGCCTCGCCATATaaaatta                                                                                                   >  1:31387/1‑146 (MQ=255)
                                                         aaaaaCGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTT‑CAGCAGCGGGTTGCGCT‑CCCTTAATGTGCCTCGCCATATAAATTGAATgg                                                                                               <  2:369330/150‑1 (MQ=255)
                                                              cGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTT‑CAGCAGCGGGTTGCGCT‑CCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAg                                                                                          <  2:3553515/150‑1 (MQ=255)
                                                                 tttGCCGAGGCCGATGCGCACTGTCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTTGTT‑CTGCAGCGGGGTTTGCT‑CCCCTTATTTGGCTCCCCCTATAAATTTAATTGGGGAgggg                                                                                       >  2:4116115/1‑149 (MQ=255)
                                                                 tttGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGCTT‑TCGCCGCGGGGTGCGCT‑CCCCTTATGTGGCTCGCCCTATAAATTTAATGGGGGAgggg                                                                                       >  2:3364606/1‑149 (MQ=255)
                                                                 tttGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTT‑CAGCAGCGGGTTGCGCT‑CCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGa                                                                                       <  1:2261065/150‑1 (MQ=255)
                                                                      cGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGCTT‑CAGCAGCGGGGTGCGCC‑CCCCTAATTTGCCTCGCCATATAAAATTAATGGGGCCAGGAgcgcg                                                                                  >  2:3906761/1‑150 (MQ=255)
                                                                       gAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGCTT‑CCGCAGCGGGGTGCGCC‑CCCCTTATTTGCCTCCCCCTATAAAATTAATTGTGCAGGGGgcgcg‑c                                                                                >  2:2278411/1‑150 (MQ=255)
                                                                       gAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGCTT‑CCGCAGCGGGGTGCGCC‑CCCCTTATGTGGCCCGCCCTATAAAATTAATTGGGCCGGGGgggcg‑c                                                                                >  2:1869240/1‑150 (MQ=255)
                                                                         ggCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑GTTTTTTTTGCTT‑CAGCAGCGGGGTGCGGC‑CCCCTTATTTGGCCCCCCATTTAAAATTAATGGTGGCGGGAGCGCG‑CAg                                                                              >  2:3213714/1‑150 (MQ=255)
                                                                          gCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTT‑CAGCAGCGGGTTGCGCT‑CCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCG‑CAgg                                                                             <  1:4158531/150‑1 (MQ=255)
                                                                              aTGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTTGGTT‑TGGACCCGGGGTGGGCT‑CCCCTTATGTGCCTCCCCCTCTAAATTTTATGGGGTGGGGAGGGCG‑CAgggggg                                                                         >  2:1999/1‑149 (MQ=255)
                                                                               tGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTT‑CAGCAGCGGGTTGCGCT‑CCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCG‑CAGGGggcg                                                                        <  1:813899/150‑1 (MQ=255)
                                                                                  gcACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGGTT‑CAGCAGCGGGGTGCGCC‑CCCCTTATGTGGCCCCCCCTATAAATTGAATGGTGCAGGGGGGGCG‑CCgggggggggc                                                                     >  2:2206058/1‑148 (MQ=255)
                                                                                  gcACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTT‑CAGCAGCGGGTTGCGCT‑CCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGcc                                                                     <  1:2648766/150‑1 (MQ=255)
                                                                                      tATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTT‑CAGCAGCGGGTTGCGCT‑CCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATc                                                                 <  1:4049287/150‑1 (MQ=255)
                                                                                         cATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGCTT‑CCGCAGCGGGGTGGGCT‑CCCCTTATTTGGCTCCCCCTATAAATTTAATTGTGGAGGGGGGGCG‑CAGGGGGGGGCCCAAcgcc                                                              >  2:3663025/1‑150 (MQ=255)
                                                                                                   cgccgTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTT‑CAGCAGCGGGTTGCGCT‑CCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCct                                                    <  2:2934327/150‑1 (MQ=255)
                                                                                                    gccgTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGGTT‑CAGCAGCGGGGTGCGCT‑CCCCTTATGTGCCTCCCCCTATAAATTTAATGGTGCAGGGGGCGCG‑CACGGGGGGGCCAAACGCCGcccccccccg                                                   >  2:3812233/1‑148 (MQ=255)
                                                                                                            ggTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGG‑TTTTTTTTGGGTT‑TA‑CAGCCGGGTGGCCT‑CCCCTGATATGCCCCGCCCTTTAAATTTAATTTTGCCGGGGGCGGG‑CCGGGGGCGGGCCATCGCCGCCGCCCCccgcccgcccc                                           >  2:1857750/1‑143 (MQ=255)
                                                                                                            ggTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTT‑CAGCAGCGGGTTGCGCT‑CCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCg                                           <  2:138216/150‑1 (MQ=255)
                                                                                                            ggTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTT‑CAGCAGCGGGTTGCGCT‑CCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCg                                           <  1:3397539/150‑1 (MQ=255)
                                                                                                             gTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTT‑CAGCAGCGGGTTGCGCT‑CCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCgg                                          <  1:479870/150‑1 (MQ=255)
                                                                                                              ttGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGGTT‑CAGCAGCGGGGTGCGCT‑CCCCTTATTTGGCTCGCCCTATAAAATTAATGGTGCAGGGGGGGCG‑CAGGGGGGGGGCAACCCCCCCCCCCCCCCCCCGTccccgg                                         >  2:438604/1‑147 (MQ=255)
                                                                                                                 cAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTT‑CAGCAGCGGGTTGCGCT‑CCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCtt                                      <  1:440001/150‑1 (MQ=255)
                                                                                                                        gCTAAGGGACTTAGCCTGCGGC‑GG‑GTTTGTTTGGGTT‑TAGCAGCGGGGGGGGGG‑CCCCTAATGTGGCTCGCCATATAAAATGAAATGGGGGGGGAGCGCGCCAGGGGGGGGCCAA‑CCCCCCCGGCCCCCGCCGTCCCCGCCGTC‑cgggga                              >  2:3198113/1‑148 (MQ=255)
                                                                                                                          tAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGGTT‑CAGCAGCGGGGTTCGCT‑CCCCTTATGTGCCTCGCCATATAAATTTAATGGTGCAGGGGGGGCG‑CAGGGGGCGGGCCAACGCCCCCCCCCCCCCCCGTCCCGGCCCTCTGGGGAAc                             >  2:3133408/1‑150 (MQ=255)
                                                                                                                          tAAGGGACTTAGCCTGCGGC‑GG‑GTTTTTTTGGCTT‑CAGCAGCGGGGGGGGGC‑CCCCTTATGTGGCCCGCCCTATAAAATTAATGGGGCGGGGGGCGGG‑CAGGGGGGGGCCAAACGCCCGCGCCCCCCGCTGGGCCCGCCCTC‑tggggggg                            >  2:3066417/1‑146 (MQ=255)
                                                                                                                              ggACTTAGCCTGCGGC‑GG‑TTTTTTTTTGGTT‑CAGCAGCGGGGTGCGCTCCCCTTATTGGGCC‑CCCCCTATAAATTTTATGGGGCAGGGGGCGCG‑CCGGGGGGGGCCCATCGCCCCCCCCCCCCCCTGTCCCCGCCCTC‑CGGGGAAGCtcc                        >  2:286171/1‑148 (MQ=255)
                                                                                                                                aCTTAGCCTGCGGC‑GG‑TTTTTTTTTGCTT‑TAGCAGCGGGGTGCGCC‑CCCCTAATTTGCCTCCCCCTATAAATTTAATGGTGCAGGGGGCGCG‑CCGGGGGGGGCCCATCGCCCCCCCCCCCCCCCGTCCCCGCCCTC‑GGGGGACGCCTCAg                      >  2:1534128/1‑150 (MQ=255)
                                                                                                                                aCTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTT‑CAGCAGCGGGTTGCGCT‑CCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTC‑GGGGAACGCTTCAg                      <  1:1285864/150‑1 (MQ=255)
                                                                                                                                 cTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTT‑CAGCAGCGGGTTGCGCT‑CCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTC‑GGGGAACGCTTCAGc                     <  1:142739/150‑1 (MQ=255)
                                                                                                                                      ccTGCGGC‑GG‑TTTTGTTTGGCTT‑CAGCAGCGGGTTGCGCT‑CCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTC‑GGGGAACGCTTCAGCGAttt                <  2:1695028/150‑1 (MQ=255)
                                                                                                                                            gc‑gg‑TTTTGTTTGGCTT‑CAGCAGCGGGTTGCGCT‑CCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTC‑GGGGAACGCTTCAGCGATTTTGACGc          <  2:198746/150‑1 (MQ=255)
                                                                                                                                            gc‑gg‑TTTTGTTTGGCTT‑CAGCAGCGGGTTGCGCT‑CCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTC‑GGGGAACGCTTCAGCGATTTTGACGc          >  1:530762/1‑150 (MQ=255)
                                                                                                                                               gg‑TTTTGTTTGGCTT‑CAGCAGCGGGTTGCGCT‑CCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTC‑GGGGAACGCTTCAGCGATTTTGACGcca        >  1:3488985/1‑150 (MQ=255)
                                                                                                                                                  ttttGTTTGGCTT‑CAGCAGCGGGTTGCGCT‑CCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTC‑GGGGAACGCTTCAGCGATTTTGACGccacc      >  1:2387996/1‑150 (MQ=255)
                                                                                                                                                  ttttGTTTGGCTT‑CAGCAGCGGGTTGCGCT‑CCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTC‑GGGGAACGCTTCAGCGATTTTGACGccacc      >  2:1655059/1‑150 (MQ=255)
                                                                                                                                                   tttgtttgGCTT‑CAGCAGCGGGTTGCGCT‑CCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTC‑GGGGAACGCTTCAGCGATTTTGACGccacca     >  2:2387149/1‑150 (MQ=255)
                                                                                                                                                   tttgtttgGCTT‑CAGCAGCGGGTTGCGCT‑CCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTC‑GGGGAACGCTTCAGCGATTTTGACGccacca     >  1:531329/1‑150 (MQ=255)
                                                                                                                                                   tttgtttgGCTT‑CAGCAGCGGGTTGCGCT‑CCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTC‑GGGGAACGCTTCAGCGATTTTGACGccacca     >  1:1130685/1‑150 (MQ=255)
                                                                                                                                                    ttgtttgGCTT‑CAGCAGCGGGTTGCGCT‑CCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTC‑GGGGAACGCTTCAGCGATTTTGACGCCACCaa    >  2:1821549/1‑150 (MQ=255)
                                                                                                                                                     tgtttgGCTT‑CAGCAGCGGGTTGCGCT‑CCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTC‑GGGGAACGCTTCAGCGATTTTGACGCCACCAac   >  1:814624/1‑150 (MQ=255)
                                                                                                                                                      gtttgGCTT‑CAGCAGCGGGTTGCGCT‑CCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTC‑GGGGAACGCTTCAGCGATTTTCACGCCACCAaca  <  2:4143247/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                         
GCAGTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTT‑CAGCAGCGGGTTGCGCT‑CCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTC‑GGGGAACGCTTCAGCGATTTTGACGCCACCAACA  >  AP012306/934906‑935203

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑