Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP012306935,0540GT27.1% 266.7 / 49.3 96intergenic (+24/‑1406)putP/ycdOproline:sodium symporter/conserved protein
Reads supporting (aligned to +/- strand):  ref base G (12/58);  new base T (26/0);  total (38/58)
Fisher's exact test for biased strand distribution p-value = 1.32e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 163 total aligned reads displayed.

GGCAGTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTTCAG‑CAG‑CGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGCCACCAACA  >  AP012306/934905‑935203
                                                                                                                                                       |                                                                                                                                                        
  cAGTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑Gtttttt                                                                                                                                                        >  2:267582/1‑150 (MQ=255)
   aGTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCTGC‑GG‑Ttttgttt                                                                                                                                                       >  1:2690372/1‑150 (MQ=255)
       ttGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCt                                                                                                                                                   <  1:162380/150‑1 (MQ=255)
       ttGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCt                                                                                                                                                   <  1:1913936/150‑1 (MQ=255)
       ttGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑GTTTTTTTTGCt                                                                                                                                                   >  2:88295/1‑150 (MQ=255)
          ggATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTTca                                                                                                                                                <  1:992266/150‑1 (MQ=255)
           gATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTGGCTTcag                                                                                                                                               >  2:689630/1‑150 (MQ=255)
           gATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑GTTTTTTTTGGTTctg                                                                                                                                               >  2:1969222/1‑148 (MQ=255)
            aTTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGCTTccga                                                                                                                                              >  2:3629925/1‑147 (MQ=255)
            aTTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑GTTTGGTTGGGTTcag‑c                                                                                                                                             >  1:1969557/1‑150 (MQ=255)
              tGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGATGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑GTTTGTTTGGCTTccg‑cag                                                                                                                                           >  1:2233763/1‑150 (MQ=255)
              tGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTTcag‑cag                                                                                                                                           <  1:3739218/150‑1 (MQ=255)
               gTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGCTTCcc‑cag‑c                                                                                                                                         >  1:1985551/1‑150 (MQ=255)
               gTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTTCag‑cag‑c                                                                                                                                         <  1:2288865/150‑1 (MQ=255)
               gTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTTCag‑cag‑c                                                                                                                                         <  2:3029378/150‑1 (MQ=255)
                 aGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTGGCTTCag‑ccg‑ccg                                                                                                                                       >  1:2900121/1‑148 (MQ=255)
                 aGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTTGCTTCAG‑CAG‑Cgg                                                                                                                                       >  1:2407092/1‑150 (MQ=255)
                 aGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTTCAG‑CAG‑Cgg                                                                                                                                       <  1:3268883/150‑1 (MQ=255)
                 aGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑GTTTTTTTTGGTTTAG‑CCG‑Cgg                                                                                                                                       >  2:4377488/1‑150 (MQ=255)
                    gtTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGG‑TTTTTTTTGGTTTcgg‑cccggggt                                                                                                                                      >  2:2222588/1‑143 (MQ=255)
                    gtTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGCTTCAG‑CAG‑Cggggt                                                                                                                                    >  1:3087397/1‑148 (MQ=255)
                     tttAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGGTTCCGCCAG‑GGGGtt                                                                                                                                    >  1:4290972/1‑150 (MQ=255)
                     tttAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGCTTTAG‑CAGCCGGGtt                                                                                                                                    >  1:1749515/1‑150 (MQ=255)
                      ttAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGCCTCTG‑CCG‑CGGGGTgc                                                                                                                                  >  1:4053908/1‑150 (MQ=255)
                        aGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑GTTTGTTTGGGTTCAG‑CAG‑CGGGGTgcgc                                                                                                                                >  1:4019093/1‑150 (MQ=255)
                         gTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑GTTTGTTTTTTTTCCG‑CAG‑Cggggttgggc                                                                                                                               >  2:3213071/1‑143 (MQ=255)
                          tttGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑GTTTTTTTTGCTTTCG‑CAG‑CGGGGTggggcc                                                                                                                              >  2:3343480/1‑145 (MQ=255)
                           ttGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGGTTTCG‑CAG‑CGGGGTGCGCTcc                                                                                                                             >  1:1074052/1‑150 (MQ=255)
                            tGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTGGCTTTCG‑CAG‑CGGGGGGCGCcccc                                                                                                                            >  2:327308/1‑150 (MQ=255)
                            tGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑CTTTGTTTTGCGTCAG‑CAG‑CGGGGGGGGCTccc                                                                                                                            >  2:137571/1‑150 (MQ=255)
                             gCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGCTTCAG‑CAG‑CGGGGTGCGCTcccc                                                                                                                           >  1:2639318/1‑149 (MQ=255)
                             gCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑CTTTGTTTGGGTTCCG‑CAG‑GGCGGTGCGCccccc                                                                                                                           >  1:980729/1‑149 (MQ=255)
                             gCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAACCTGCGGC‑GG‑GTTTTTTTGGGTTCCG‑CAG‑CGGGGTgccgccccc                                                                                                                           >  2:2684058/1‑143 (MQ=255)
                              cTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGCTTTAG‑CAG‑CGGGGTGCGCTcccct                                                                                                                          >  1:855535/1‑148 (MQ=255)
                                   tAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTTCAG‑CAG‑CGGGTTGCGCTCCCTTAAtgt                                                                                                                     <  1:292015/150‑1 (MQ=255)
                                     aaGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑GTTTTTTTGGGTTTTG‑CAG‑CGGGGGTCGCTCCCCTTAtgtgg                                                                                                                   >  2:3462321/1‑149 (MQ=255)
                                      aGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTTCAG‑CAG‑CGGGTTGCGCTCCCTTAATGTGcc                                                                                                                  <  2:1733938/150‑1 (MQ=255)
                                         gcCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGGTTCCG‑GAG‑CGGGGTGGGCGCCCTTTATGTGCCCCg                                                                                                               >  1:225103/1‑150 (MQ=255)
                                         gcCGTCAGCGGAGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTTCAG‑CAG‑CGGGTTGCGCTCCCTTAATGTGCCTCg                                                                                                               <  1:2915008/150‑1 (MQ=255)
                                          ccGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGG‑TTTTTTTTGGTTTC‑G‑CAG‑CGGGGGGGGCTCCCCTTATGTGCCTCGc                                                                                                              >  2:20626/1‑150 (MQ=255)
                                          ccGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGGTTTAG‑CAG‑CGGGGTGTGCTCCCCTTATGTGCCTccc                                                                                                              >  2:2365189/1‑148 (MQ=255)
                                           cGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGCTTCCG‑CAG‑CGGGGTTCGCTCCCCTAAtttggcccccc                                                                                                             >  1:1642325/1‑143 (MQ=255)
                                            gTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGGTTCTG‑CAG‑GGGGGGGCGCCCCCCTTATTTGGCTCGccc                                                                                                            >  2:587783/1‑149 (MQ=255)
                                            gTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTTCAG‑CAG‑CGGGTTTCGCTCCCTTAATGTGCCTCGCCa                                                                                                            >  1:607623/1‑150 (MQ=255)
                                             tCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGGTTCCA‑CAA‑CCGGGTGCGCCCCCTTTATTTGCCCCGccca                                                                                                           >  1:784011/1‑148 (MQ=255)
                                                 cggcgATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTTGCTTCAG‑GAG‑CGGGGTGCGCTCCCCTAATTTGCCTCCCCCTATaa                                                                                                       >  1:1080692/1‑150 (MQ=255)
                                                 cggcgATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTTCAG‑CAG‑CGGGTTGCGCTCCCTTAATGTGCCTCGCCATATaa                                                                                                       <  1:3743551/150‑1 (MQ=255)
                                                 cggcgATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTTCAG‑CAG‑CGGGTTGCGCTCCCTTAATGTGCCTCGCCATATaa                                                                                                       <  2:553205/150‑1 (MQ=255)
                                                  ggcgATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTTCAG‑CAG‑CGGGTTGCGCTCCCTTAATGTGCCTCGCCATATaaa                                                                                                      <  2:216240/150‑1 (MQ=255)
                                                    cgATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTTCAG‑CAG‑CGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAAtt                                                                                                    <  2:1301778/150‑1 (MQ=255)
                                                      aTGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTTGTTTCG‑CAT‑CGGGGTTGGCTCCCTTTTTTTTGCTCGCCATTTaaaatta                                                                                                  >  2:1270983/1‑146 (MQ=255)
                                                         cAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTTCAG‑CAG‑CGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATg                                                                                               <  1:3557436/150‑1 (MQ=255)
                                                           aaagCGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTTCAG‑CAG‑CGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGt                                                                                             <  1:3578938/150‑1 (MQ=255)
                                                            aaaCGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTTGCTTCTG‑CAG‑CGGGGTGGGCCCCCCTTATTTGCCTCGCCCTCTAAAATTAATGGTg                                                                                            >  1:894209/1‑150 (MQ=255)
                                                                gCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTTCAG‑CAG‑CGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAgg                                                                                        <  2:2858593/150‑1 (MQ=255)
                                                                    tGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTTCAG‑CAG‑CGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAgc                                                                                    <  2:1545583/150‑1 (MQ=255)
                                                                      ccGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGGTTCCG‑CAG‑CGGGGTGCGGTTCCTTTATTTTCCTCGCCCTTAAAAATGAATTGGGCCGGGAgcgc                                                                                  >  2:1728828/1‑150 (MQ=255)
                                                                              gATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGATTTTTTTATGCTT‑AT‑GGG‑CGGGCTACCGGCCCCTAATGTGCCTCCCCCAATAAATTGAATGGTGGAGGGAGCGCG‑CAggggg                                                                         >  2:627018/1‑150 (MQ=255)
                                                                              gATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGGTTTCG‑CAG‑CGGGGTGCGCTCCCCTTATTTGGCCCCCCCTTTAAATTTTATTGGGCAGGGGGCGCG‑CCggggg                                                                         >  2:3517323/1‑150 (MQ=255)
                                                                               aTGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGCTTCAG‑CAG‑CGGGGTGGGCTCCCCTTATGTGCCTCGCCCTATAAATTTTATGGTGGAGGGGGCGCG‑CAgggggg                                                                        >  2:830114/1‑149 (MQ=255)
                                                                                tGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑GTTTTTTTTGCTTCAG‑CAG‑CGGGGTGCGCCCCCCTTATGTGGCCCCCCCTATTAATTTAATTGGGGAAGGGGGGCG‑CCGGGggcg                                                                       >  2:145902/1‑150 (MQ=255)
                                                                                   gcACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTTCAG‑CAG‑CGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGcc                                                                    <  2:646940/150‑1 (MQ=255)
                                                                                       tATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑GTTTTTTTTGCTTCAG‑CAG‑CGGGGTGCGCCCCCCTAATGTGGCTCCCCCTATAAATTTAATGGTGGAGGGGGCGCG‑CAGGGGGGGGcccaac                                                                >  2:86187/1‑146 (MQ=255)
                                                                                          cATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTTCAG‑CAG‑CGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATcgcc                                                             <  2:3341897/150‑1 (MQ=255)
                                                                                                  tCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTGGCTTCTG‑CAG‑CGGGGGGCGCTCCCCTAATTTGCGTCGCCCTCTAAATTTAATGGTGCAGGGGGGGCG‑CCGGGGGGGGCCAATCGCCGCcccccc                                                     >  2:93678/1‑150 (MQ=255)
                                                                                                   ccgccgTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGGTTCCG‑CAG‑CGGGGTGGGCTCCCTTTATTTGCCCCGCCCTATAAAATTAATGGTGCAGGGGGCGCG‑CAGGGGGGGGCCAAACCCCGcccccccc                                                    >  2:4078139/1‑150 (MQ=255)
                                                                                                     gccgTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTTCAG‑CAG‑CGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCctg                                                  <  2:2185252/150‑1 (MQ=255)
                                                                                                       cgTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGGTTCTG‑CAG‑CGGGGTGGGCCCCCCTTATTTGCCTCGCCCTATAAAATTAATTGTGCAGGGGGGGCG‑CCGGGGGGGGGCCAACGCCCCCCCCCCccgcc                                                >  2:2375009/1‑149 (MQ=255)
                                                                                                          cACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGGTTTCG‑CCA‑CGGGGTGGGCTCCCCTATTTTGGCCCCCCCTATAAATTTAATTGGGGAGGGGGCGCG‑CCCGGGGGGGGCAAACGCCGCCCCCCCCCGCCGtt                                             >  2:4314144/1‑149 (MQ=255)
                                                                                                             ggTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTTCAG‑CAG‑CGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCg                                          <  2:386037/150‑1 (MQ=255)
                                                                                                                  cAGGAAAGCTAAGGGACTTAGCCTGCTGC‑GG‑TTTTGTTTGGCTTCAG‑CAG‑CGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCtt                                     <  2:2690372/150‑1 (MQ=255)
                                                                                                                  cAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTTCAG‑CAG‑CGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCtt                                     <  1:3011468/150‑1 (MQ=255)
                                                                                                                   aGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTTCAG‑CAG‑CGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTc                                    <  2:374888/150‑1 (MQ=255)
                                                                                                                    ggAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGGTTTAG‑CAG‑CCGGGGGGGCTCCCCTAATGTGGCCCCCCCCATAAATTGAATGGGGCAGGGAGCGCG‑CCGGGGGGGGGCAATCGCCCCCGCCCCCCCCCGTCCCCGcccccg                                   >  2:1517159/1‑146 (MQ=255)
                                                                                                                    ggAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTTCAG‑CAG‑CGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCg                                   <  1:869158/150‑1 (MQ=255)
                                                                                                                     gAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGCTTTCG‑CAG‑CGGGGTGCGCTCCCTTTATGTGGCTCCCCATTTTAATTTAATTGGGGCGGGGGGGCG‑CCGGGGGGGGGCCAACCCCCCCCCCCCCTGCTGTCCCCGCCTTccg                                  >  2:301643/1‑148 (MQ=255)
                                                                                                                       aaGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGGTTCAG‑CAG‑CGGGGTGCGCTCCCCTAATTTGCCTCGCCCTATAAATTTAATGGTGCCGGGGGCGCG‑CAGGGGGGGGCCCATCCCCCCCCCCCCCCGCTGTCCCGGCCCTCcggg                                >  2:1342177/1‑150 (MQ=255)
                                                                                                                            aaGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGCTTCAG‑CAA‑CGGGGTTGGCTCCCCTTATTTGGCTCGCCCTATAAAATTAATGGGGGAGGGGGGGCGCCGGGGGGGGGCCAACCCCCCCCCCCCCCCCCTTCCCCGCCCTCCGGGGaagc                            >  2:2558643/1‑148 (MQ=255)
                                                                                                                             aGGGACTTAGCCTGCGGC‑GG‑GTTTTTTTGGGTTCAG‑GAG‑GGGGGTGGGGGCCCCTAAAGGGGCTCGCCATATAAAATGGAAGGGGGAGGGGGGGCG‑GAGGGGGGGGGCAATCGCCGCCGCCCCCCGCTGTCCCCGCCTTCgggggaagcg                          >  2:1783728/1‑144 (MQ=255)
                                                                                                                               ggACTTAGCCTGCGGC‑GG‑TTTTTTTTTGCTTTTT‑CCGCCGGGGGGCCGCCCCTTAA‑ATGTCTCTGCATATAAAATTTATGGTGGTGGGAGGGGG‑CGGGGGGGGGCCAAACTCCGCCGCCCCCTGCTTTTCCTGCCCTCCGGGGACGCttt                        >  2:2406778/1‑149 (MQ=255)
                                                                                                                                gACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTTCAG‑CAG‑CGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCa                       <  2:3328537/150‑1 (MQ=255)
                                                                                                                                 aCTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTTCAG‑CAG‑CGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAg                      <  1:3105146/150‑1 (MQ=255)
                                                                                                                                  cTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTTCAG‑CAG‑CGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGc                     <  1:2425283/150‑1 (MQ=255)
                                                                                                                                  cTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTTCAG‑CAG‑CGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGc                     <  2:3107989/150‑1 (MQ=255)
                                                                                                                                      gCCTGCGGC‑GG‑TTTTGTTTGGCTTCAG‑CAG‑CGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGAtt                 <  1:2927877/150‑1 (MQ=255)
                                                                                                                                           cggc‑gg‑TTTTGTTTGGCTTCAG‑CAG‑CGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCTTTCGGGGAACGCTTCAGCGATTTTGAc            <  2:2050210/150‑1 (MQ=255)
                                                                                                                                            ggc‑gg‑TTTTGTTTGGCTTCAG‑CAG‑CGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACg           >  1:3957854/1‑150 (MQ=255)
                                                                                                                                            ggc‑gg‑TTTTGTTTGGCTTCAG‑CAG‑CGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACg           <  2:1963769/150‑1 (MQ=255)
                                                                                                                                            ggc‑gg‑TTTTGTTTGGCTTCAG‑CAG‑CGGGGTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGGACGCTTCAGCGCTTTTGACg           >  2:388452/1‑150 (MQ=255)
                                                                                                                                              c‑gg‑TTTTGTTTGGCTTCAG‑CAG‑CGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGcc         >  2:2636425/1‑150 (MQ=255)
                                                                                                                                              c‑gg‑TTTTGTTTGGCTTCAG‑CAG‑CGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGcc         <  2:3493357/150‑1 (MQ=255)
                                                                                                                                                gg‑TTTTGTTTGGCTTCAG‑CAG‑CGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGcca        >  1:1823613/1‑150 (MQ=255)
                                                                                                                                                   ttttGTTTGGCTTCAG‑CAG‑CGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGccacc      >  2:1192862/1‑150 (MQ=255)
                                                                                                                                                   ttttGTTTGGCTTCAG‑CAG‑CGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGccacc      >  1:252663/1‑150 (MQ=255)
                                                                                                                                                    tttgtttgGCTTCAG‑CAG‑CGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGccacca     <  2:1148103/150‑1 (MQ=255)
                                                                                                                                                    tttgtttgGCTTCAG‑CAG‑CGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGccacca     <  1:2925323/150‑1 (MQ=255)
                                                                                                                                                    tttgtttgGCTTCAG‑CAG‑CGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGccacca     >  2:3631746/1‑150 (MQ=255)
                                                                                                                                                    tttgtttgGCTTCAG‑CAG‑CGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGccacca     >  2:2789181/1‑150 (MQ=255)
                                                                                                                                                    tttgtttgGCTTCAG‑CAG‑CGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGccacca     <  1:1698434/150‑1 (MQ=255)
                                                                                                                                                       gtttgGCTTCAG‑CAG‑CGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGCCACCAaca  >  2:2943169/1‑150 (MQ=255)
                                                                                                                                                       |                                                                                                                                                        
GGCAGTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTTCAG‑CAG‑CGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGCCACCAACA  >  AP012306/934905‑935203

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑