Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123061,128,4120TG24.5% 696.6 / 40.4 286V29G (GTG→GGG) yciOconserved protein
Reads supporting (aligned to +/- strand):  ref base T (51/165);  new base G (70/0);  total (121/165)
Fisher's exact test for biased strand distribution p-value = 6.01e-34
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 337 total aligned reads displayed.

CCGCAGGCGTTGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAA‑TTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAACTCTCGACCTATT  >  AP012306/1128263‑1128561
                                                                                                                                                     |                                                                                                                                                      
     ggCGTTGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTg                                                                                                                                                   <  2:575293/150‑1 (MQ=255)
     ggCGTTGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTg                                                                                                                                                   <  2:2311047/150‑1 (MQ=255)
     ggCGTTGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTg                                                                                                                                                   <  1:3085286/150‑1 (MQ=255)
     ggCGTTGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTg                                                                                                                                                   <  1:585477/150‑1 (MQ=255)
      gCGTTGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGt                                                                                                                                                  >  2:2730577/1‑150 (MQ=255)
            aaGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATcc                                                                                                                                            >  1:2757651/1‑150 (MQ=255)
                cGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACt                                                                                                                                        <  1:4362859/150‑1 (MQ=255)
                  tCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTTTCCAACTGa                                                                                                                                      >  1:985239/1‑150 (MQ=255)
                  tCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGa                                                                                                                                      <  2:2581382/150‑1 (MQ=255)
                  tCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGa                                                                                                                                      <  2:2158421/150‑1 (MQ=255)
                     ggCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTc                                                                                                                                   <  1:3789188/150‑1 (MQ=255)
                            aTGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTAt                                                                                                                            <  1:3153666/150‑1 (MQ=255)
                             tGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGGTATg                                                                                                                           >  1:894223/1‑150 (MQ=255)
                             tGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATg                                                                                                                           <  1:4257478/150‑1 (MQ=255)
                                gAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTTTCCAAATGATTCCGGCTAttggc                                                                                                                        >  2:1128657/1‑146 (MQ=255)
                                gAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAACTGATTCCGGCTATgcgc                                                                                                                        >  2:1398517/1‑150 (MQ=255)
                                gAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAACTGATTCCGGCTATgcgc                                                                                                                        >  1:1266577/1‑150 (MQ=255)
                                  aCAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAACTGATTTCGGCTATTCGCTc                                                                                                                      >  2:4248078/1‑150 (MQ=255)
                                   cAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGGTTTTTTTTTCAACTGCTTTCGGCTATGcgcgcg                                                                                                                     >  2:656694/1‑150 (MQ=255)
                                    aGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCgg                                                                                                                    <  2:4276103/150‑1 (MQ=255)
                                     gCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGc                                                                                                                   <  2:1175203/150‑1 (MQ=255)
                                      ccGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCt                                                                                                                  <  1:351760/150‑1 (MQ=255)
                                        gCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGt                                                                                                                >  1:4012962/1‑150 (MQ=255)
                                         cAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTa                                                                                                               <  1:655406/150‑1 (MQ=255)
                                            aaCACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTaaaa                                                                                                            <  1:2884268/150‑1 (MQ=255)
                                            aaCACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTaaaa                                                                                                            <  1:3889014/150‑1 (MQ=255)
                                               accacAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCCAATGATTCCGGCTATGCGCTCGGCTGTAAAA‑TTg                                                                                                        >  1:937304/1‑150 (MQ=255)
                                                 cacaGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAA‑TTGaa                                                                                                      <  2:407808/150‑1 (MQ=255)
                                                    agagagGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAA‑TTGAAGAt                                                                                                   <  1:1798691/150‑1 (MQ=255)
                                                    agagagGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAA‑TTGAAGAt                                                                                                   <  1:1434044/150‑1 (MQ=255)
                                                     gagagGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAA‑TTTAAGATa                                                                                                  >  1:1020839/1‑150 (MQ=255)
                                                     gagagGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAA‑TTTAAGATa                                                                                                  >  2:3490573/1‑150 (MQ=255)
                                                      agagGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCCACTGGTTCCGGCTATGCGCTCGGCTGTGAAA‑TTTAAGATaa                                                                                                 >  2:3962598/1‑150 (MQ=255)
                                                      agagGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAA‑TTGAAGATaa                                                                                                 <  2:520363/150‑1 (MQ=255)
                                                       gagGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAA‑TTGAAGATaaa                                                                                                <  1:1839877/150‑1 (MQ=255)
                                                       gagGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAA‑TTGAAGATaaa                                                                                                <  1:2057678/150‑1 (MQ=255)
                                                        agGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAA‑TTGAAGATaaaa                                                                                               <  1:2107801/150‑1 (MQ=255)
                                                         gggAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGGTTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAA‑TTGAAGATaaaaa                                                                                              >  2:2407000/1‑150 (MQ=255)
                                                          ggAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAAATGATTCCGGCTATGCGCTCGGCTGTAAAA‑TTGAAGATaaaaaa                                                                                             >  2:2003947/1‑149 (MQ=255)
                                                           gAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTTTCCCACTGATTTCGGCTATTCGCCCGGCTGTAAAATTtaaaaataaaaag                                                                                             >  2:3218202/1‑149 (MQ=255)
                                                           gAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAACTGATTCCGGCTATGGGCTCGGCTGTAAAA‑TTGAAGATaaaaaag                                                                                            >  1:2616267/1‑148 (MQ=255)
                                                            aaTTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAAATGATTCCGGCTATGCGCTCGGGTGTAAAA‑ATTAAGATAAAAAcgc                                                                                           >  2:1745986/1‑150 (MQ=255)
                                                             aTTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAA‑TTGAAGATAAAAAcgcg                                                                                          >  1:4279358/1‑150 (MQ=255)
                                                             aTTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAA‑TTGAAGATAAAAAcgcg                                                                                          >  1:162028/1‑150 (MQ=255)
                                                               tATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAA‑TTGAAGATAAAAACGCGAt                                                                                        <  1:4206881/150‑1 (MQ=255)
                                                               tATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAA‑TTGAAGATAAAAACGCGAt                                                                                        <  1:4347299/150‑1 (MQ=255)
                                                                aTGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAA‑TTGAAGATAAAAACGCGATg                                                                                       <  2:812263/150‑1 (MQ=255)
                                                                 tGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTTTCCCACTGATTCCGGCTATGCGCTCGGCTGTAAAA‑TTTAAGATAAAAAAGCGGTgg                                                                                      >  2:1815290/1‑150 (MQ=255)
                                                                 tGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAA‑TTGAAGATAAAAAAGCGATgg                                                                                      >  2:3310717/1‑150 (MQ=255)
                                                                      cAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAA‑TTGAAGATAAAAACGCGATGGAGCGt                                                                                 <  2:3713270/150‑1 (MQ=255)
                                                                      cAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAACTGATTTCGGCTATGCGCTCGGCTGTAAAA‑TTGAAGATAAAAAAGCGATGGAGCGt                                                                                 >  2:3532815/1‑150 (MQ=255)
                                                                       aGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAA‑TTGAAGATAAAAACGCGATGGGGCGTa                                                                                >  1:2441023/1‑150 (MQ=255)
                                                                        gTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAA‑TTGAAGATAAAAACGCGATGGAGCGTAt                                                                               <  1:2837959/150‑1 (MQ=255)
                                                                        gTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAA‑TTGAAGATAAAAACGCGATGGAGCGTAt                                                                               <  2:3585397/150‑1 (MQ=255)
                                                                         tttttttATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAA‑TTGAAGATAAAAACGCGATGGAGCGTAtt                                                                              <  1:2493880/150‑1 (MQ=255)
                                                                           tttttATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAA‑TTGAAGATAAAAACGCGATGGAGCGTATTTg                                                                            >  1:330355/1‑150 (MQ=255)
                                                                            ttttATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGGAAAA‑TTGAAGATAAAAACGCGATGGAGCGTAttttt                                                                           >  1:21728/1‑148 (MQ=255)
                                                                            ttttATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAA‑TTGAAGATAAAAACGCGATGGAGCGTAttttt                                                                           >  1:2289176/1‑148 (MQ=255)
                                                                             tttATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAA‑TTGAAGATAAAAACGCGATGGAGCGTATTTGTc                                                                          <  2:3055302/150‑1 (MQ=255)
                                                                              ttATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAA‑TTGAAGATAAAAACGCGATGGAGCGTATTTGTCg                                                                         <  1:3442228/150‑1 (MQ=255)
                                                                              ttATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAA‑TTGAAGATAAAAACGCGATGGAGCGTATTTGTCg                                                                         <  1:3610050/150‑1 (MQ=255)
                                                                              ttATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAA‑TTGAAGATAAAAACGCGATGGAGCGTATTTGTCg                                                                         <  1:3911332/150‑1 (MQ=255)
                                                                               tataTTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGCAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAA‑TTGAAGATAAAAACGCGATGGAGCGTATTTGTCGt                                                                        >  2:4223312/1‑150 (MQ=255)
                                                                                 tatTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAA‑TTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTAt                                                                      >  2:1511269/1‑150 (MQ=255)
                                                                                   ttCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAA‑TTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTc                                                                    <  1:1561685/150‑1 (MQ=255)
                                                                                    tCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAA‑TTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCg                                                                   <  1:255975/150‑1 (MQ=255)
                                                                                     cATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAA‑TTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGc                                                                  <  1:3670376/150‑1 (MQ=255)
                                                                                        ccTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAA‑TTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAg                                                               >  2:629587/1‑150 (MQ=255)
                                                                                             tAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTTTTTATCCCACTTATTCCGGCTATGCGCTCGGGTGTAAAA‑TTGAAGATAAAAACGCGGTGGGGCGTATTTGTCGTATTCGCCAGCTGcc                                                          >  2:2256500/1‑150 (MQ=255)
                                                                                               aCCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAA‑TTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCCGCTGCCgg                                                        >  1:2278868/1‑150 (MQ=255)
                                                                                                cccACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAA‑TTGAAGATAAAAACGCGATGGAGCGTATTTTTCGTATTCGCCAGCTGCCGGa                                                       >  1:2335659/1‑150 (MQ=255)
                                                                                                 ccACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAA‑TTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGAc                                                      >  2:1848400/1‑150 (MQ=255)
                                                                                                  cacaGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAA‑TTGAAGATAAAAACGCGATGGGGCGTATTTGTCGTATTCGCCAGCTGCCCGACg                                                     >  2:1061428/1‑150 (MQ=255)
                                                                                                    caGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAA‑TTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGt                                                   <  2:3930944/150‑1 (MQ=255)
                                                                                                    caGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAA‑TTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGt                                                   <  1:3212849/150‑1 (MQ=255)
                                                                                                      gCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAA‑TTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTca                                                 <  2:930576/150‑1 (MQ=255)
                                                                                                       cAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCCGCTATGCGCTCGGCTGTAAAA‑TTGAAGATAAAAACCCGATGGAGCGTATTTGTCGTATTCGCCACCTGCCGGACGGTcac                                                >  1:1510342/1‑150 (MQ=255)
                                                                                                           gTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTTTCCAACTGATTCCGGCTATGCGCTCGGCTGGAAAA‑TTGGAGATAAAAACGCGATGGAGCGGATTTGTCGTATTCGCCAGCTGCCGGACGGGCACAACt                                            >  2:3171578/1‑150 (MQ=255)
                                                                                                              tGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAA‑TTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTa                                         <  2:2348389/150‑1 (MQ=255)
                                                                                                              tGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGGAAAA‑TTGAAGATAAAAACGCGATGGAGCGGATTTGTCGTATTCGCCAGCTGCCGGACGGGCACAACTTTa                                         >  2:3468897/1‑150 (MQ=255)
                                                                                                               gATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAA‑TTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTAc                                        >  1:114715/1‑150 (MQ=255)
                                                                                                                 tCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAA‑TTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTAccc                                      <  1:3492962/150‑1 (MQ=255)
                                                                                                                 tCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAA‑TTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTAccc                                      <  1:4075747/150‑1 (MQ=255)
                                                                                                                 tCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAA‑TTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTAccc                                      >  1:4139770/1‑150 (MQ=255)
                                                                                                                          cGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAA‑TTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTc                             >  2:4034125/1‑150 (MQ=255)
                                                                                                                           ggTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAA‑TTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCCGCTGCCGGACGGTCACAACTTTACCCTGGTGTGTcg                            >  2:618370/1‑150 (MQ=255)
                                                                                                                             tGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAA‑TTGAAGATAAAAACGCGATGGAGCGTATTTGTTGTATTCGCCAGCTGCCGGACGGTCACAAATTTACCCTGATGTGTcgcg                          >  2:3725446/1‑150 (MQ=255)
                                                                                                                              ggAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAA‑TTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGa                         >  1:3709463/1‑150 (MQ=255)
                                                                                                                                agaTCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAA‑TTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATc                       <  2:725329/150‑1 (MQ=255)
                                                                                                                                agaTCGTGCGTAAAGGCGGGGGGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAA‑TTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATc                       >  1:523302/1‑150 (MQ=255)
                                                                                                                                 gaTCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAA‑TTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCt                      >  2:1228328/1‑150 (MQ=255)
                                                                                                                                         gTAAAGGCGGGGGGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCCGTAAAA‑TTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAAc              >  1:1870778/1‑150 (MQ=255)
                                                                                                                                           aaaGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAA‑TTGAAGATAAAAACGCGATGGAGCGCATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAActc            >  1:1171783/1‑150 (MQ=255)
                                                                                                                                            aaGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAA‑TTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAActct           >  2:2905744/1‑150 (MQ=255)
                                                                                                                                             aGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAA‑TTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAACtctc          <  2:1304419/150‑1 (MQ=255)
                                                                                                                                             aGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAA‑TTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAACtctc          <  2:380535/150‑1 (MQ=255)
                                                                                                                                             aGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAA‑TTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAACtctc          <  2:168240/150‑1 (MQ=255)
                                                                                                                                             aGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAA‑TTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAACtctc          <  2:192224/150‑1 (MQ=255)
                                                                                                                                                   ggTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAA‑TTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAACTCTCGACCTa    <  2:2973274/150‑1 (MQ=255)
                                                                                                                                                     tGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAA‑TTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAACTCTCGACCTAtt  <  1:2970323/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
CCGCAGGCGTTGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAA‑TTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAACTCTCGACCTATT  >  AP012306/1128263‑1128561

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑