Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123061,226,5570AG27.6% 404.2 / 86.6 196G55G (GGA→GGG) ydcUpredicted spermidine/putrescine transporter subunit
Reads supporting (aligned to +/- strand):  ref base A (18/124);  new base G (54/0);  total (72/124)
Fisher's exact test for biased strand distribution p-value = 5.07e-33
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 217 total aligned reads displayed.

TTGCAATCACCTTCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCAGCGCCATTCTGG  >  AP012306/1226408‑1226705
                                                                                                                                                     |                                                                                                                                                    
   cAATCACCTTCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGttt                                                                                                                                                   >  2:1662224/1‑150 (MQ=255)
    aaTCACCTTCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGtttt                                                                                                                                                  >  2:2786445/1‑150 (MQ=255)
     aTCACCTTCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTta                                                                                                                                                 <  2:1223113/150‑1 (MQ=255)
      tccccTTCACGTCCAGGTCTGGGTAAGGGGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTtat                                                                                                                                                <  2:460838/147‑1 (MQ=255)
      tCACCTTCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTtat                                                                                                                                                <  2:2271704/150‑1 (MQ=255)
       cACCTTCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTtata                                                                                                                                               <  1:3086568/150‑1 (MQ=255)
       cACCTTCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTtata                                                                                                                                               <  2:2053412/150‑1 (MQ=255)
        aCCTTCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATAc                                                                                                                                              <  2:3059893/150‑1 (MQ=255)
          cTTCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTATACtt                                                                                                                                            >  1:1548198/1‑150 (MQ=255)
           ttCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGGTTTATAAttt                                                                                                                                           >  1:1397128/1‑150 (MQ=255)
           ttCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACttt                                                                                                                                           <  2:2645879/150‑1 (MQ=255)
            tCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCTGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACtttt                                                                                                                                          <  2:579051/150‑1 (MQ=255)
               cGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGAc                                                                                                                                       <  1:4226314/150‑1 (MQ=255)
               cGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCCCGCTGCTGACACTGTTATGGCAGGGGTTTTTTTCttttttc                                                                                                                                       >  2:3387935/1‑147 (MQ=255)
                gTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACttttttct                                                                                                                                      >  1:1523378/1‑146 (MQ=255)
                 tCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTACTTTTTACGa                                                                                                                                     >  2:673723/1‑150 (MQ=255)
                 tCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGa                                                                                                                                     <  2:2299222/150‑1 (MQ=255)
                 tCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGa                                                                                                                                     <  1:1843229/150‑1 (MQ=255)
                 tCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGa                                                                                                                                     <  2:1912971/150‑1 (MQ=255)
                   cAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGAtt                                                                                                                                   <  2:3986819/150‑1 (MQ=255)
                      gTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTACTTTTTACGAttttt                                                                                                                                >  2:1617422/1‑148 (MQ=255)
                      gTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCa                                                                                                                                <  1:1080178/150‑1 (MQ=255)
                         tGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGGTTTTTTTTTTTGACGATTTcttca                                                                                                                             >  2:2901792/1‑146 (MQ=255)
                         tGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGGTTTTTTCTTTTTTCGATTTTAccc                                                                                                                             >  2:2591910/1‑149 (MQ=255)
                          gggTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCAt                                                                                                                            <  2:916126/150‑1 (MQ=255)
                            gTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTATTCTTTTTACGATTTCACCCTGt                                                                                                                          >  1:3783644/1‑150 (MQ=255)
                            gTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTATACTTTTTACGATTTCACCATGt                                                                                                                          >  1:2401278/1‑150 (MQ=255)
                               aGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTTTACTTTTGACGATTTCACCATGTCgg                                                                                                                       >  1:840708/1‑150 (MQ=255)
                                ggTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGt                                                                                                                      <  1:2884485/150‑1 (MQ=255)
                                 gtgtCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTa                                                                                                                     <  1:1871592/150‑1 (MQ=255)
                                   gtCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAAc                                                                                                                   <  1:3565082/150‑1 (MQ=255)
                                       ggTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGGTTTTTATTTTTTACGATTTTACCCTGTCGGTAAAGCCg                                                                                                               >  2:3196116/1‑150 (MQ=255)
                                          ttcttcTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTACTTTTTACGATTTTACCATGTCGGTAACGCCggga                                                                                                            >  1:205597/1‑148 (MQ=255)
                                          ttcttcTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTATACTTTTGACGATTTCACCATGTCCGTAACGCCCGaa                                                                                                            >  1:3139685/1‑150 (MQ=255)
                                           tcttctGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAAc                                                                                                           <  1:243144/150‑1 (MQ=255)
                                                tGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTATTCTTTTGTCGATTTCCCCCTGTCGGTAAAGCCGGAAATGACg                                                                                                      >  2:747071/1‑150 (MQ=255)
                                                    aTAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTACTTTTTACGGTTTTACCATGTCGGTAACGCCCGGACTGACGCTgg                                                                                                  >  1:1291673/1‑150 (MQ=255)
                                                     tAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGc                                                                                                 <  2:1236184/150‑1 (MQ=255)
                                                      aaTCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCg                                                                                                <  2:2165144/150‑1 (MQ=255)
                                                       aTCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGa                                                                                               <  2:1578047/150‑1 (MQ=255)
                                                         ccGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAat                                                                                             <  1:2294026/150‑1 (MQ=255)
                                                            gggctggggctgTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTTCTTTTTGCGATTTCCCCCTGTCGGTAACGCCGGAACTGACGCCGGCGAATATc                                                                                          >  1:1985994/1‑150 (MQ=255)
                                                             ggctggggctgTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTACTTTTTTCGATTTTCCCCTTTCGGGAAAGCCGGGACTGACGCTGGCGAatattc                                                                                         >  2:343775/1‑148 (MQ=255)
                                                             ggctggggctgTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATcc                                                                                         <  2:804397/150‑1 (MQ=255)
                                                             ggctggggctgTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATcc                                                                                         <  2:4228141/150‑1 (MQ=255)
                                                                        tttttACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGttt                                                                              <  1:3887262/150‑1 (MQ=255)
                                                                        tttttACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGttt                                                                              <  1:1805448/150‑1 (MQ=255)
                                                                           ttACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAAt                                                                           <  1:210355/150‑1 (MQ=255)
                                                                           ttACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCC‑GAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATc                                                                          <  1:3525793/150‑1 (MQ=255)
                                                                            tACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTATACTTTTTACGATTTCACCATGTCGGTAACGCCGGGACTGACGCTGGCGAATATCCGTGCGCTGTTTTATc                                                                          >  2:1507790/1‑150 (MQ=255)
                                                                               tgctgcTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTACTTTTTACGATTTTACCCTTTCGGGAACGCCGGGACTGAAGCTGGCGAAAATCCCTGCGCTGTTTTATcccg                                                                       >  2:3725724/1‑148 (MQ=255)
                                                                                    ctTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTACTTTTTTCGATTTTACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAAt                                                                  >  2:813546/1‑150 (MQ=255)
                                                                                    ctTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAAt                                                                  <  2:3273149/150‑1 (MQ=255)
                                                                                     ttGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAAtt                                                                 <  1:3498685/150‑1 (MQ=255)
                                                                                       ggCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTAc                                                               <  2:2310022/150‑1 (MQ=255)
                                                                                               aaTGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGAcatcat                                                       <  1:2697135/150‑1 (MQ=255)
                                                                                               aaTGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGAcatcat                                                       <  1:874577/150‑1 (MQ=255)
                                                                                               aaTGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGAcatcat                                                       <  2:3373325/150‑1 (MQ=255)
                                                                                                 tgtgGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATtc                                                     >  1:4083550/1‑150 (MQ=255)
                                                                                                 tgtgGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGGTTTTTACTTTTTACGATTTTACCATATCGGTAACGCCGGGACTGACGCTGGCGAATATTCCTGCGCTGTTTAATCCCGCGAATTACGACATCATtc                                                     >  2:514185/1‑150 (MQ=255)
                                                                                                 tgtgGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATtc                                                     <  1:922830/150‑1 (MQ=255)
                                                                                                  gtgGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGATTTTTACTTTTTACGATTTTACCCTATCGGTAACGCCGGAACTTACGCCGGCGAAAATCCCTGCGCTTTTTAATACCGCGGATTACGACATCATtct                                                    >  2:3041021/1‑150 (MQ=255)
                                                                                                  gtgGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATtct                                                    <  2:3641044/150‑1 (MQ=255)
                                                                                                   tgGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATtctc                                                   <  2:3905767/150‑1 (MQ=255)
                                                                                                    ggTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTcc                                                  <  2:4095483/150‑1 (MQ=255)
                                                                                                    ggTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTcc                                                  <  2:29328/150‑1 (MQ=255)
                                                                                                    ggTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTcc                                                  <  1:1271567/150‑1 (MQ=255)
                                                                                                     gTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTACTTTTTACGATTTCACCATGTCGGTAACGCCGGGACTGACGCTGGCGAATATCCCTGCGCTGTTTTATCCCGCGAATTACGACATCCTTCTCCg                                                 >  2:626593/1‑150 (MQ=255)
                                                                                                      tttGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTTCTTTTTACGATTTCACCATGTCGGGAAAGCCCGGAATGACGCTGGCGGATATCCGTGCGCTGTTTTATTCGGCGAATTACGACATCATTCTCCGc                                                >  2:3527836/1‑150 (MQ=255)
                                                                                                        tGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCAc                                              <  2:2075693/150‑1 (MQ=255)
                                                                                                        tGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCAc                                              <  2:3563445/150‑1 (MQ=255)
                                                                                                              tGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGAc                                        <  2:1356098/150‑1 (MQ=255)
                                                                                                               gTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTACTTTTTACGATTTTACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTTATTCGGCGAATTACGACATCATTCTCCGCACGCTGACt                                       >  2:2364068/1‑150 (MQ=255)
                                                                                                               gTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACt                                       <  1:1497866/150‑1 (MQ=255)
                                                                                                                 cTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTAt                                     <  2:1051742/150‑1 (MQ=255)
                                                                                                                    tttCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTACTTTTTACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTTATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGc                                  >  1:4261004/1‑150 (MQ=255)
                                                                                                                    tttCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGc                                  <  2:2859862/150‑1 (MQ=255)
                                                                                                                       cGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTATTTTTTGCGGATTTACCCTGTCGGGAACACCGGAAATTACGCCGGCGAAGATCCGCGCGCCTGTTAATTCGCCCGATTACGACATCATTCTCCGCACCCCGGCTTTCGCtgt                               >  2:200253/1‑150 (MQ=255)
                                                                                                                         gCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTTACGATTTCACCATGTCCGTAACGCCCGAACTGACGCTGGCGAAAATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCCCACGCTGACTATGGCTGTgg                             >  2:1352287/1‑150 (MQ=255)
                                                                                                                         gCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTgg                             <  1:2928637/150‑1 (MQ=255)
                                                                                                                         gCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTgg                             <  1:2762531/150‑1 (MQ=255)
                                                                                                                         gCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTgg                             >  1:2185583/1‑150 (MQ=255)
                                                                                                                           tCGCTGCTGACACTGTTATGGCAGGGGTTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCa                           >  1:100167/1‑150 (MQ=255)
                                                                                                                             gctgctGACACTGTTATGGCAGGGGTTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGt                         >  1:3954415/1‑150 (MQ=255)
                                                                                                                               tgctgACACTGTTATGGCAGGGGTTTTATACTTTTGACGGTTTCACCATGTCGGTAACGCCGGGACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGcggggc                       >  2:1281682/1‑145 (MQ=255)
                                                                                                                               tgctgACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCa                       >  1:1734269/1‑150 (MQ=255)
                                                                                                                                gctgACACTGTTATGGCAGGGGTTTTATACTTTTGACGATTTCACCATGTCGGTAAAGCCCGAACTGACGCTGGCGAATATCCGGGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACCCTGACTATGGCTGTGGCCGTCAc                      >  2:1726989/1‑150 (MQ=255)
                                                                                                                                  tgACACTGTTATGGCAGGGGTTTTTTACTTTTTACGGTTTTACCATGTCGGTAACGCCGGGAATGACGCTGGCGAATATCCGTGCGCTGTTTTATCCTGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTTAcca                    >  2:3965553/1‑148 (MQ=255)
                                                                                                                                   gACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTAt                   <  1:2123308/150‑1 (MQ=255)
                                                                                                                                   gACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTAt                   <  2:3015771/150‑1 (MQ=255)
                                                                                                                                      acTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGc                <  1:2316998/150‑1 (MQ=255)
                                                                                                                                       cTGTTATGGCAGGGGTTTTATACTTTTGACGATTTCACCATGTCGGGAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGcc               >  1:1676217/1‑150 (MQ=255)
                                                                                                                                        tGTTATGGCAGGGGTTTTATACTTTTTACGATTTCACCATGTCGGTAACGCCGGGACTGACGCTGGCGAATATCCGTGCGCTGTTTTATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGGCACCATCGccc              >  2:1406844/1‑149 (MQ=255)
                                                                                                                                          ttATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCAgc            <  2:873330/150‑1 (MQ=255)
                                                                                                                                           tATGGCAGGGGTTTTATACTTTTGACGATTTTACCCTGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTTTCCCCACGCTGACTATGGCTGTGGCCGGCACTATCTCCAgcg           >  2:3252109/1‑150 (MQ=255)
                                                                                                                                            aTGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCAgccc          >  2:2068859/1‑148 (MQ=255)
                                                                                                                                               gCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCAGCGCCAt       <  2:1865655/150‑1 (MQ=255)
                                                                                                                                                cAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCAGCGCCAtt      >  1:1036623/1‑150 (MQ=255)
                                                                                                                                                  gggATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCAGCGCCATTCt    <  2:699078/150‑1 (MQ=255)
                                                                                                                                                    gATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCAGCGCCATTCTgg  <  2:2287603/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TTGCAATCACCTTCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCAGCGCCATTCTGG  >  AP012306/1226408‑1226705

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑