Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123063,938,8870CT28.8% 491.2 / 157.9 257S44F (TCT→TTT) bglJDNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (25/158);  new base T (74/0);  total (99/158)
Fisher's exact test for biased strand distribution p-value = 3.31e-43
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 307 total aligned reads displayed.

ACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGA‑‑ACT‑GGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACG  >  AP012306/3938738‑3939036
                                                                                                                                                      |                                                                                                                                                         
aCTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGCC‑Ttttttc                                                                                                                                                           >  1:3343862/1‑150 (MQ=255)
   cATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑Ttttttcttt                                                                                                                                                        <  1:3126762/150‑1 (MQ=255)
    aTAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCC‑Ttttttttttt                                                                                                                                                       >  2:4146304/1‑150 (MQ=255)
    aTAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑Ttttttctttt                                                                                                                                                       <  1:1096676/150‑1 (MQ=255)
      aGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑Ttttttctttttc                                                                                                                                                     <  1:4382885/150‑1 (MQ=255)
       gAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTttttctttttct                                                                                                                                                    <  1:128373/150‑1 (MQ=255)
        aaaaaTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCC‑ttttttttttttttt                                                                                                                                                   >  2:1992302/1‑147 (MQ=255)
           aaTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑ttttttttttttttttca                                                                                                                                                >  2:2393466/1‑144 (MQ=255)
            aTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCAt                                                                                                                                               <  2:250397/150‑1 (MQ=255)
             tGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATg                                                                                                                                              <  2:3378395/150‑1 (MQ=255)
              gCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATga                                                                                                                                             <  1:3456521/150‑1 (MQ=255)
              gCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATga                                                                                                                                             <  1:2294952/150‑1 (MQ=255)
                  cATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTTTTTTTTTTTCCTGAGaaa                                                                                                                                         >  2:2223133/1‑149 (MQ=255)
                    tGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCC‑TTTTTTTTTTTTTTTCCATTTGAAGTg                                                                                                                                       >  2:3112427/1‑150 (MQ=255)
                     gAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGa                                                                                                                                      <  2:420829/150‑1 (MQ=255)
                      agtagtATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTTTTCCATTAGAAATGAg                                                                                                                                     >  1:875355/1‑150 (MQ=255)
                      agtagtATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAg                                                                                                                                     <  1:1012038/150‑1 (MQ=255)
                      agtagtATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTATGCCGTGGTCT‑GCTTTTAAATTTATTGCATGAGAAATGAg                                                                                                                                     >  1:2085074/1‑150 (MQ=255)
                       gtagtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGCA‑TTTTTTTTTTTTTTTCCATGAGAAGTGAgg                                                                                                                                    >  2:557143/1‑149 (MQ=255)
                        tagtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCACTTTTTTTTTTTTTTGCCTTG‑GAAGTGAgag                                                                                                                                   >  1:4076015/1‑148 (MQ=255)
                        tagtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAgcg                                                                                                                                   <  2:679305/150‑1 (MQ=255)
                         agtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAgcgc                                                                                                                                  <  1:2430271/150‑1 (MQ=255)
                           taTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTTTTTTTTTTCCATGAGAAGTGAGAGCag                                                                                                                                >  1:813433/1‑150 (MQ=255)
                            aTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTTTTTTTTTTCCCTGAGAAGTGAGGGCaga                                                                                                                               >  1:2715467/1‑150 (MQ=255)
                             tCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTTTTTTTTTGTCATGAGAAGTGAGCGCagag                                                                                                                              >  2:593693/1‑150 (MQ=255)
                             tCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCagag                                                                                                                              <  2:4282485/150‑1 (MQ=255)
                               ggTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTTTTTTTCTTCCCCGAGAAATGAGCGCagagag                                                                                                                            >  1:4336720/1‑150 (MQ=255)
                               ggTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGCCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTTTGCCATGAGAAGTGGgcgccgcaag                                                                                                                            >  2:89575/1‑144 (MQ=255)
                                 tATTGAGAGTTTATTCAGAAAGTTTGCGGGTGACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAggg                                                                                                                          <  1:3883269/150‑1 (MQ=255)
                                 tATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCC‑TTTTTTTTTTTTTTGGCCTGAGAAGGGAGGGCagggaggg                                                                                                                          >  1:1432092/1‑150 (MQ=255)
                                 tATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAggg                                                                                                                          <  1:690100/150‑1 (MQ=255)
                                     gagaGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTa                                                                                                                      <  1:1658813/150‑1 (MQ=255)
                                       gagTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTTTTCCCTGAGAAGTGAGCGCCGAGAGGGGGTATc                                                                                                                    >  1:3060096/1‑150 (MQ=255)
                                        agTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTTTTTTTTTTCCCTTAGAAATGAGGGCCGAGAGGGGTTAttt                                                                                                                   >  1:3502227/1‑148 (MQ=255)
                                        agTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTTTTTTTTTTCCCTGAGAAGGGAGGGCAGAGAgggggttttt                                                                                                                   >  2:1520233/1‑143 (MQ=255)
                                        agTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCt                                                                                                                   <  1:1857241/150‑1 (MQ=255)
                                         gTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCtt                                                                                                                  <  2:2062724/150‑1 (MQ=255)
                                         gTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCtt                                                                                                                  <  1:762964/150‑1 (MQ=255)
                                          tttATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTTTTTTTTTTCCATGAGAAGGGAGCGCCGAGAGGGGTTATCttt                                                                                                                 >  1:3281477/1‑149 (MQ=255)
                                            tATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCC‑TTTTTTTTTTTTTTTCCATGAGAAGGGAGCGCCGAGGGGGGTTATCTTTcc                                                                                                               >  1:2856724/1‑150 (MQ=255)
                                            tATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCC‑TTTTTTCTTTTTCTTCCATTAGGAGGGAGCGCAGCGAGGGGTTATCTTggc                                                                                                               >  2:918254/1‑148 (MQ=255)
                                             aTTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCct                                                                                                              <  1:529928/150‑1 (MQ=255)
                                                 aGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTTTTTTTTTTCCATGGGAAGGGAGCGCAGAGAGGGGTTATCTTTCctgact                                                                                                          >  1:2974496/1‑150 (MQ=255)
                                                 aGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTTTTTTTCTTCCATGAGAAGTGAGGGCGGAGAGGGGTTATTTTTCctgact                                                                                                          >  2:331273/1‑150 (MQ=255)
                                                 aGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCctgact                                                                                                          <  1:3848553/150‑1 (MQ=255)
                                                 aGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCctgact                                                                                                          <  2:258047/150‑1 (MQ=255)
                                                 aGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCctgact                                                                                                          <  2:1306241/150‑1 (MQ=255)
                                                   aaaGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTTTTTTTTTTCCATTGGGAGATAGCGCAGGGAGGGATTATCTTGTctgagtga                                                                                                        >  2:272585/1‑150 (MQ=255)
                                                      gTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCC‑TTTTTTTTTTTTTTTCCCTGAGAAGTGGGCGCCGGGAGGGGTTATCTTGCCTGTCCGA‑‑ACt                                                                                                   >  1:2592297/1‑150 (MQ=255)
                                                      gTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTTTTTTTCTTTCATGCGAAGTGAGCGCAGAGAGGGACTATCTTACcttactgcaat                                                                                                     >  1:1568941/1‑146 (MQ=255)
                                                         tGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGA‑‑ACT‑GGc                                                                                               <  2:3872204/150‑1 (MQ=255)
                                                          gCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCT‑TTTTTTTTTTTTTTGCCATGAGAAGGGGGGGCAGGGAGGGATTATCTTGTATGACTGA‑‑ACT‑GGCg                                                                                              >  2:2644930/1‑150 (MQ=255)
                                                           cGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑GTTTTTCTTTTTTTGCCATGAGAAGGGGGCGGAGGGGGGGGGTATCTTGCCTCACTGA‑‑ACA‑TGCGa                                                                                             >  1:1214437/1‑150 (MQ=255)
                                                               tAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGA‑‑ACT‑GGCGA‑TTaa                                                                                        <  1:468628/150‑1 (MQ=255)
                                                                aaCCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGA‑‑ACT‑GGCGA‑TTAAg                                                                                       <  2:1584783/150‑1 (MQ=255)
                                                                       ataAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGA‑‑ACT‑GGCGA‑TTAAGTTTCcgc                                                                                <  2:1711395/150‑1 (MQ=255)
                                                                          aGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCC‑TTTTTTTTTTTTTTTCCCTGAGAAGAGAGGGGAGAGGGGGGTTATCTTGCCTGACTGA‑‑ACT‑GGCGATTTAAGTTTcgcgca                                                                              >  2:1626002/1‑145 (MQ=255)
                                                                          aGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGA‑‑ACT‑GGCGA‑TTAAGTTTCCGCGTa                                                                             <  1:3808816/150‑1 (MQ=255)
                                                                              ccATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGA‑‑ACT‑GGCGA‑TTAAGTTTCCGCGTACCCg                                                                         <  2:2114838/150‑1 (MQ=255)
                                                                              ccATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGA‑‑ACT‑GGCGA‑TTAAGTTTCCGCGTACCCg                                                                         <  2:3022941/150‑1 (MQ=255)
                                                                                aTACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGA‑‑ACT‑GGCGA‑TTAAGTTTCCGCGTACCCGGc                                                                       <  1:1600389/150‑1 (MQ=255)
                                                                                   ccTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGA‑‑ACT‑GGCGA‑TTAAGTTTCCGCGTACCCGGCGtt                                                                    <  1:984682/150‑1 (MQ=255)
                                                                                    cTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGCA‑TTTTTTTTTTTTTTTCCATGAGAAGTGAGCGGAGAGAGGGATTATCTTTCCTGACTGG‑‑ACT‑GGGGG‑ATTAGATTTCGCGTACCCGGCGttt                                                                   >  2:2311564/1‑150 (MQ=255)
                                                                                      ataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGA‑‑ACT‑GGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAg                                                                 <  1:1147508/150‑1 (MQ=255)
                                                                                       taCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGA‑‑ACT‑GGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGt                                                                <  2:2859111/150‑1 (MQ=255)
                                                                                         ccAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGCA‑TTTTTTTTTTTTTTGCCATGAGAAGGGAGCGCAGAGAGGGGTTATTTTTCCTGACTGA‑‑ACTGGGGGT‑TTAAGTTTCC‑CGTACCCCGCGGTTTGttt                                                              >  2:168478/1‑149 (MQ=255)
                                                                                            gTCAGGAGTCATTTCAGGATGCCATGTCGCGTATCTGGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGATTGA‑‑ACT‑GGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTg                                                           <  2:406270/150‑1 (MQ=255)
                                                                                            gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGA‑‑ACT‑GGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTg                                                           <  2:2127120/150‑1 (MQ=255)
                                                                                             tCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTTTTTTTTTGCCATGAGAAGTGAGCGCAGAGAGGGGGTATCTTTCCTGGCTTA‑‑ACT‑GGCGG‑TTTAATTTTCCCGGACCCCGCGGTTTGTtttttc                                                          >  2:3573003/1‑148 (MQ=255)
                                                                                             tCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGCG‑TTTTTTTTTTTTTTGCCATGAGAAGTGAGGGCGGAGGGGGGATATCTTGCCCGTCTTA‑‑ACT‑GGCGG‑TTTAGTTTTCGCGGACCCCGCGCTTTGTTAtttc                                                          >  2:1197878/1‑148 (MQ=255)
                                                                                               aGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGA‑‑ACT‑GGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCgg                                                        <  1:3742820/150‑1 (MQ=255)
                                                                                               aGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGA‑‑ACT‑GGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCgg                                                        <  2:1128239/150‑1 (MQ=255)
                                                                                                  aGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGA‑‑ACT‑GGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgatg                                                     <  1:3390410/150‑1 (MQ=255)
                                                                                                   gTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGA‑‑ACT‑GGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgatga                                                    <  2:3515159/150‑1 (MQ=255)
                                                                                                     cATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTTTTTTTTTTTCCTGAGAAGTGAGCGCCGAGAGCGGGTATCTTTCCTGACTGA‑‑ACT‑GGCGA‑TTTAGTTTTCCCGGACCCCGCGGTTTTTTATTTCGggtgatg                                                  >  2:3744279/1‑150 (MQ=255)
                                                                                                     cATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGA‑‑ACT‑GGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgatgatg                                                  <  1:2638417/150‑1 (MQ=255)
                                                                                                      aTTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGA‑‑ACT‑GGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgatgatga                                                 <  2:2343946/150‑1 (MQ=255)
                                                                                                      aTTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGA‑‑ACT‑GGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgatgatga                                                 <  1:661323/150‑1 (MQ=255)
                                                                                                       tttCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGA‑‑ACT‑GGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgatgatgat                                                <  2:3078101/150‑1 (MQ=255)
                                                                                                       tttCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGA‑‑ACT‑GGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgatgatgat                                                <  2:3988459/150‑1 (MQ=255)
                                                                                                        ttCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGA‑‑ACT‑GGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGata                                               <  1:1980554/150‑1 (MQ=255)
                                                                                                         tCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGA‑‑ACT‑GGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGatat                                              <  1:2402397/150‑1 (MQ=255)
                                                                                                            ggATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTTTTTTTTTTCCCCGGGAAGTGAGCGCAGAGAGGGATTTTCTTTTCTGACGGAGGTCT‑GGCG‑‑TTAAGTTT‑CGCGTGCTCGGCGGTTTGTTTTTTTGGGTGATGATAtttt                                           >  2:1878278/1‑148 (MQ=255)
                                                                                                               tGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGA‑‑ACT‑GGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGc                                        <  2:2539928/150‑1 (MQ=255)
                                                                                                                   aTGTCGCGGATCTCGTTTGCGGCGGGGA‑GTTTTTTTTTTTTTGGCATGAGGAGGGGGGGGGGGGAGGGGGTATGTTGGCTGACTGA‑‑AAT‑GGGGG‑GTAAAGTTTCGCGGACCCGGGGGGTAGGTATTGGGGAGGGGGAGATTGaaaggcgg                                    >  2:1393567/1‑144 (MQ=255)
                                                                                                                            aTCTCGTTTGCGGCGGTCA‑TTTTTTTTTTTTTTGCCCTGAGGAGGGAGGGCAGAGAGGGGTTATTTTTCCTGACTGA‑‑ACT‑GGCGA‑TTTAGTTTTCCCGGTCCCCGCGGTTTGTTATTTCGGGTGATGGTATTTTAACTCCGCCGATTTGt                           >  2:484919/1‑150 (MQ=255)
                                                                                                                            aTCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGGTTATCTTGCCTGACTGA‑‑ACT‑GGCGA‑TTAAGTTTCCGCGTACCCGGCGGTTTGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGt                           >  1:1459798/1‑150 (MQ=255)
                                                                                                                               tcGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGA‑‑ACT‑GGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCa                        <  1:3657307/150‑1 (MQ=255)
                                                                                                                                cGTTTGCGGCGGTCA‑TTTTTTTTTTTTTTTCCATGAGAAGGGAGGGCAGAGGGGGGTTATCTTTTCTGACTTA‑‑ACT‑GGCGG‑TTAAGTTTTCGCGTACCCCGCGGTTTGTTTTTTTGGATGATGATATTTTAACTCGGCTGGTTTGttttt                       >  2:3826772/1‑147 (MQ=255)
                                                                                                                                   ttGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGA‑‑ACT‑GGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGt                    <  2:3008254/150‑1 (MQ=255)
                                                                                                                                        gcggTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGA‑‑ACT‑GGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCa               >  2:3655712/1‑150 (MQ=255)
                                                                                                                                         cggTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGA‑‑ACT‑GGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCAt              >  2:3045004/1‑150 (MQ=255)
                                                                                                                                         cggTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGA‑‑ACT‑GGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCAt              <  1:2769490/150‑1 (MQ=255)
                                                                                                                                          ggTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGA‑‑ACT‑GGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATc             <  2:126667/150‑1 (MQ=255)
                                                                                                                                             cA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGA‑‑ACT‑GGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCAcc          <  1:3253286/150‑1 (MQ=255)
                                                                                                                                                 tttttctttttcTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGA‑‑ACT‑GGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCTCCGCt       >  2:3239138/1‑150 (MQ=255)
                                                                                                                                                  ttttctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGA‑‑ACT‑GGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTg      <  2:827732/150‑1 (MQ=255)
                                                                                                                                                  ttttctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGA‑‑ACT‑GGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTg      <  2:1442807/150‑1 (MQ=255)
                                                                                                                                                   tttctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGA‑‑ACT‑GGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTgg     <  2:3421004/150‑1 (MQ=255)
                                                                                                                                                    ttctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGA‑‑ACT‑GGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGa    <  1:3947847/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                         
ACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGA‑‑ACT‑GGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACG  >  AP012306/3938738‑3939036

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑