Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP012306202,4170TG25.9% 609.4 / 29.3 263V409G (GTA→GGA) tilStRNA(Ile)‑lysidine synthetase
Reads supporting (aligned to +/- strand):  ref base T (83/112);  new base G (68/0);  total (151/112)
Fisher's exact test for biased strand distribution p-value = 1.57e-20
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 304 total aligned reads displayed.

TTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGC‑GGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTACCACCACCGTACC  >  AP012306/202268‑202565
                                                                                                                                                      |                                                                                                                                                    
   cAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGC‑GGCGGCAGGGGTAtt                                                                                                                                                   <  1:1604728/150‑1 (MQ=255)
    aaaGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGC‑GGCGGCAGGGGTAttt                                                                                                                                                  <  1:3352880/150‑1 (MQ=255)
    aaaGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGC‑GGCGGCAGGGGGAttt                                                                                                                                                  >  1:543545/1‑150 (MQ=255)
     aaGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGC‑GGCGGCAGGGGTATTtg                                                                                                                                                 <  1:3411907/150‑1 (MQ=255)
       gcgcCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGGGGCTACGTGGCACCACGCCCCTGCTGTTTTATGGCGAAACGCTGGTTGCGGGGGGCAGGGGCTTTtgg                                                                                                                                                >  2:3098874/1‑149 (MQ=255)
         gcCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGCGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGC‑GGCGGGCGGGGGAATTTTGAc                                                                                                                                             >  2:334994/1‑150 (MQ=255)
          ccAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGC‑GGCGGCAGGGGTATTTGTGACg                                                                                                                                            <  2:791717/150‑1 (MQ=255)
           cAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGC‑GGCGGCAGGGGGATTTGTGACGc                                                                                                                                           >  1:396378/1‑150 (MQ=255)
            aGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGC‑GGCGGCAGGGGGATTTGTGACGCa                                                                                                                                          >  1:2286849/1‑150 (MQ=255)
             ggATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGC‑GGCGGGAGGGGGGTTTTTGgcgcca                                                                                                                                         >  2:61778/1‑148 (MQ=255)
             ggATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGC‑GGCGGCAGGGGGATTTGTGACGCaa                                                                                                                                         >  1:834779/1‑150 (MQ=255)
               aTTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGC‑GGCGGCAGGGGGATTTTTGACGCaaga                                                                                                                                       >  2:4052205/1‑150 (MQ=255)
                  gctgcATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGC‑GGCGGCAGGGGGATTTGTGACGCAAGAAgg                                                                                                                                    >  1:4065289/1‑150 (MQ=255)
                   ctgcATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGC‑GGCGGGCGGGGGAGTTTTGGCCCAAAAAggg                                                                                                                                   >  2:1854260/1‑149 (MQ=255)
                   ctgcATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGC‑GGCGGCAGGGGTATTTGTGGCGCAAGAAgga                                                                                                                                   >  1:572500/1‑149 (MQ=255)
                      cATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGC‑GGCGGCAGGGGTATTTGTGACGCAAGAAGGtgtg                                                                                                                                <  1:3217851/150‑1 (MQ=255)
                      cATATTGTCGGGCGTAACGGCGGACGTAAACCAAAGAAAATCTTGCAAGAACGGGGGGGGGCGCCGTGGCTACGGGACAACACACCAATTCGGTTTTATGGAGAAAAGCTGCTTCG‑GGCGGCATGGGTATTTGTGTAGTAAGAAggatgg                                                                                                                                >  2:2288791/1‑146 (MQ=255)
                       atatTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGC‑GGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTgg                                                                                                                               <  2:1893545/150‑1 (MQ=255)
                          ttGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGC‑GGCGGCAGGGGGATTTGTGACGCAAGAAGGTGTGGCTg                                                                                                                            >  1:1708545/1‑150 (MQ=255)
                            gTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATTGCGAAACGCTGATTGC‑GGCGGGAGGGGGATTTTTGTAGCCAGAAAGGGTGGCTgga                                                                                                                          >  2:4332151/1‑148 (MQ=255)
                            gTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGC‑GGCGGCAGGGGGATTTGTGACGCAAGAAGGTGTGGCTgga                                                                                                                          >  1:1949821/1‑148 (MQ=255)
                                      aCGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGC‑GGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATg                                                                                                                <  1:1064101/150‑1 (MQ=255)
                                      aCGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGC‑GGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATg                                                                                                                >  1:3913142/1‑150 (MQ=255)
                                      aCGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGC‑GGCGGCAGGGGGATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATg                                                                                                                >  1:2126606/1‑150 (MQ=255)
                                           ggACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGC‑GGCGGCAGGGGGATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCgga                                                                                                           >  2:1759703/1‑148 (MQ=255)
                                              cGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGC‑GGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGt                                                                                                        >  1:2736106/1‑150 (MQ=255)
                                              cGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGC‑GGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGt                                                                                                        <  2:2456100/150‑1 (MQ=255)
                                               gTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGC‑GGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGtt                                                                                                       >  1:1764952/1‑150 (MQ=255)
                                                tAACCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCCGTTTTATGGCGAAACGCTGATTGC‑GGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGttt                                                                                                      <  2:1969634/150‑1 (MQ=255)
                                                  aGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGC‑GGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTg                                                                                                    >  1:202907/1‑150 (MQ=255)
                                                   gCTAAAGAAAATCTGGAAAGAGCTGGGCGTGCCGCCGTGGCTACGTGGCACCACCACACTGCTGTTTTATGGCGAAATGCTGATTGC‑GGCGGCTGGGGTATTTTTAGCGTCAGAAGGATTGGCTTATAGAGTGAAAGGCGCAAGTTTTGt                                                                                                   >  2:3927280/1‑150 (MQ=255)
                                                    cTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGC‑GGCGGCAGGGGTATTTGTGACGCAAGAAGGGGTGGCTGAAGGGGGGAATGGCGTAAGTTTTGTc                                                                                                  >  2:3705156/1‑150 (MQ=255)
                                                      aaaGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGC‑GGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTg                                                                                                <  1:1151579/150‑1 (MQ=255)
                                                       aaGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGC‑GGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTgg                                                                                               <  1:14783/150‑1 (MQ=255)
                                                       aaGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGC‑GGCGGCAGGGGGATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTgg                                                                                               >  1:2832144/1‑150 (MQ=255)
                                                         gAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGC‑GGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCa                                                                                             <  2:139471/150‑1 (MQ=255)
                                                         gAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGC‑GGCGGCAGGGGTATTTGTGACGCAAGAAGGAGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCa                                                                                             >  1:1548427/1‑150 (MQ=255)
                                                          aaaaTCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGC‑GGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAg                                                                                            >  1:1974666/1‑150 (MQ=255)
                                                          aaaaTCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGC‑GGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAg                                                                                            >  1:59504/1‑150 (MQ=255)
                                                          aaaaTCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGC‑GGCGGCAGGGGGATTTGTGAAGCAAGAAGGAGTGGGTGAAGGGGGGAAAGGCGTTAGTTTTGGCTTGCAg                                                                                            >  2:2183250/1‑150 (MQ=255)
                                                          aaaaTCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGC‑GGCGGCAGGGGAATTTTTGACGCAAGAAGGTGAGGCTGGAGGGGGGAATGGCGTAAGTTTTGTATTGCAg                                                                                            >  2:1700240/1‑150 (MQ=255)
                                                            aaTCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGC‑GGCGGCAGGGGGATTTGTGACGCAAGAAGGAGTGGCTGAAGGTGGGAATGGGGTATGGTTTGTGTTGCAGaa                                                                                          >  2:1549830/1‑150 (MQ=255)
                                                              tCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGC‑GGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGGGAGAATGGCGTAAGTTTTGTCTGGCAGaaaa                                                                                        >  2:3436928/1‑150 (MQ=255)
                                                               cTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGC‑GGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAAc                                                                                       <  2:1522741/150‑1 (MQ=255)
                                                               cTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGC‑GGCGGCAGGGGGATTTGTGACGCAAGAAGGGGTGGCTGAAGGGGAGAATGGCGGAAGTTTTGTCTGGCAGAAAAc                                                                                       >  2:2597507/1‑150 (MQ=255)
                                                                 ggCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGC‑GGCGGCAGGGGGATTTGTGACGCAAGAAGGTGTGGCTGAAGGGGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGc                                                                                     >  2:2527081/1‑150 (MQ=255)
                                                                  gCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGC‑GGCGGCAGGGGTATTTGTGACGCCAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCt                                                                                    >  1:2899025/1‑150 (MQ=255)
                                                                   cAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGC‑GGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCtt                                                                                   >  1:3615370/1‑150 (MQ=255)
                                                                   cAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGC‑GGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAAAGCtt                                                                                   >  2:2810308/1‑150 (MQ=255)
                                                                       agCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGC‑GGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGtt                                                                               >  2:2972151/1‑150 (MQ=255)
                                                                        gCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGC‑GGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTa                                                                              <  1:3160582/150‑1 (MQ=255)
                                                                        gCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCAAAACGCTGATTGC‑GGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTa                                                                              <  1:785175/150‑1 (MQ=255)
                                                                         cTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGC‑GGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTaa                                                                             <  2:4094356/150‑1 (MQ=255)
                                                                          tGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGC‑GGCGGCAGGGGGATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAg                                                                            >  1:3047829/1‑150 (MQ=255)
                                                                           gggCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGC‑GGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGt                                                                           >  1:1039756/1‑150 (MQ=255)
                                                                           gggCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGC‑GGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGt                                                                           >  1:252358/1‑150 (MQ=255)
                                                                             gCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGC‑GGCGGCAGGGGGATTTGTGACGCAAGAAGGGGTGGCTGAAAGGGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGGTAAGTGa                                                                         >  2:1097958/1‑150 (MQ=255)
                                                                              cGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGC‑GGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGaa                                                                        <  2:1269037/150‑1 (MQ=255)
                                                                                   cgccgTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGC‑GGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCg                                                                   >  1:905050/1‑150 (MQ=255)
                                                                                   cgccgTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGC‑GGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCg                                                                   <  1:740827/150‑1 (MQ=255)
                                                                                     ccgTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGC‑GGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGa                                                                 <  1:1206804/150‑1 (MQ=255)
                                                                                         ggCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGC‑GGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAg                                                             <  2:3016769/150‑1 (MQ=255)
                                                                                         ggCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGC‑GGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAg                                                             <  2:2065552/150‑1 (MQ=255)
                                                                                         ggCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGC‑GGCGGCAGGGGGATTTGTGACGCAAGAAGGTGTGGCTGAAGGGGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAg                                                             >  2:3217091/1‑150 (MQ=255)
                                                                                          gCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGC‑GGCGGCAGGGGTATTTGTGACGCAAGAAGGGGTGGCTGAAGGGGGGAATGGGGTAAGGTTTGTTTGGCAGAAAAAGCTTAGTTAAGTGAAAGCCGGATAAGa                                                            >  2:1028924/1‑150 (MQ=255)
                                                                                           cTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGC‑GGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGAc                                                           <  1:1260898/150‑1 (MQ=255)
                                                                                            tACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGC‑GGCGGCAGGGGGATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACg                                                          >  2:1670978/1‑150 (MQ=255)
                                                                                                tGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGC‑GGCGGCAGGGGGATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATc                                                      >  2:286360/1‑150 (MQ=255)
                                                                                                 gACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGC‑GGCGGCAGGGGGATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCa                                                     >  2:1202596/1‑150 (MQ=255)
                                                                                                  acacCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGC‑GGCGGCAGGGGGATTTGTGACGCAAGAAGGTGTGGCTGAAGGGGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCaa                                                    >  1:1003007/1‑150 (MQ=255)
                                                                                                      cacGCCACTGCTGTTTTATGGCGAAACGCTGATTGC‑GGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGt                                                <  2:4113110/150‑1 (MQ=255)
                                                                                                         gCCACTGCTGTTTTATGGCGAAACGCTGATTGC‑GGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGGGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGc                                             >  2:3613023/1‑150 (MQ=255)
                                                                                                          ccACTGCTGTTTTATGGCGAAACGCTGATTGC‑GGCGGCAGGGGGATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCa                                            >  2:434392/1‑150 (MQ=255)
                                                                                                           cACTGCTGTTTTATGGCGAAACGCTGATTGC‑GGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCAt                                           <  2:2403148/150‑1 (MQ=255)
                                                                                                           cACTGCTGTTTTATGGCGAAACGCTGATTGC‑GGCGGCAGGGGTATTTGTGACGCAAGAAGGGGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCAt                                           >  1:873800/1‑150 (MQ=255)
                                                                                                           cACTGCTGTTTTATGGCGAAACGCTGATTGC‑GGCGGCAGGGGGATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCAt                                           >  2:2695347/1‑150 (MQ=255)
                                                                                                              tgctgTTTTATGGCGAAACGCTGATTGC‑GGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCg                                        <  1:382367/150‑1 (MQ=255)
                                                                                                                ctgTTTTATGGCGAAACGCTGATTGC‑GGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGc                                      >  1:1391136/1‑150 (MQ=255)
                                                                                                                   ttttATGGCGAAACGCTGATTGC‑GGCGGCAGGGGGATTTGTGACGCAAGAAGGTGTGGCTGAAGGGGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGaa                                   >  2:466420/1‑150 (MQ=255)
                                                                                                                     ttATGGCGAAACGCTGATTGC‑GGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAg                                 <  1:3555045/150‑1 (MQ=255)
                                                                                                                       aTGGCGAAACGCTGATTGC‑GGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTc                               <  2:4261187/150‑1 (MQ=255)
                                                                                                                       aTGGCGAAACGCTGATTGC‑GGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTc                               >  1:1786511/1‑150 (MQ=255)
                                                                                                                          gCGAAACGCTGATTGC‑GGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAAt                            <  1:1656357/150‑1 (MQ=255)
                                                                                                                          gCGAAACGCTGATTGC‑GGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAAt                            <  1:2097613/150‑1 (MQ=255)
                                                                                                                                 gCTGATTGC‑GGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGAtt                     <  1:309494/150‑1 (MQ=255)
                                                                                                                                 gCTGATTGC‑GGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGAtt                     >  1:1919592/1‑150 (MQ=255)
                                                                                                                                  cTGATTGC‑GGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTc                    >  1:2155541/1‑150 (MQ=255)
                                                                                                                                    gATTGC‑GGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGc                  >  2:3737933/1‑150 (MQ=255)
                                                                                                                                     aTTGC‑GGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCt                 >  1:3241559/1‑150 (MQ=255)
                                                                                                                                             cggcAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTaccacc          >  1:2432317/1‑150 (MQ=255)
                                                                                                                                             cggcAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTaccacc          <  1:2855362/150‑1 (MQ=255)
                                                                                                                                             cggcAGGGGTATTTGTGACGCAAGAAGGCGTGGCTGAAGGTGAGCATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTaccacc          >  1:2706094/1‑150 (MQ=255)
                                                                                                                                                cAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTaccaccacc       >  1:4212534/1‑150 (MQ=255)
                                                                                                                                                 aGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTACCACCACCg      >  1:486783/1‑150 (MQ=255)
                                                                                                                                                 aGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTACCACCACCg      >  1:2970357/1‑150 (MQ=255)
                                                                                                                                                 aGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTACCACCACCg      <  1:4357451/150‑1 (MQ=255)
                                                                                                                                                    ggTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTACCACCACCGTAc   >  2:841578/1‑150 (MQ=255)
                                                                                                                                                    ggTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTACCACCACCGTAc   >  1:1584145/1‑150 (MQ=255)
                                                                                                                                                     gTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAATGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTACCACCACCGTAcc  >  1:4009628/1‑150 (MQ=255)
                                                                                                                                                     gTATTTGTGACGCAAGAAGGTGTGGCTGAAGGAGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTACCACCACCGTAcc  >  2:4002347/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                    
TTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGC‑GGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTACCACCACCGTACC  >  AP012306/202268‑202565

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑