BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AP012306	2,091,771	G→T	A77E (GCA→GAA)	cysM ←	cysteine synthase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AP012306	2,091,771	0	G	T	100.0%	957.1 / NA	252	A77E (GCA→GAA)	cysM	cysteine synthase B
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (128/124); total (128/124)

Only 100 of 255 total aligned reads displayed.

CCAGATCGCGCGCACCTTCCATGCCCTGCTCTTTGGTGACAAGAATCAGTTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTGCCAGCGCAATGCCGGTGTTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGTTATTGCCTTCCAGTTTTAA  >  AP012306/2091625‑2091916

ccAGATCGCGCGCACCTTCCATGCCCTGCTCTTTGGTGACAAGAATCAGTTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTTCCa                                                                                                                                                >  2:2873148/1‑150 (MQ=255)

 cAGATCGCGCGCACCTTCCATGCCCTGCTCTTTGGTGACAAGAATCAGTTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTTCCAg                                                                                                                                               >  2:2373757/1‑150 (MQ=255)

 cAGATCGCGCGCACCTTCCATGCCCTGCTCTTTGGTGACAAGAATCAGTTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTTCCAg                                                                                                                                               <  2:3499301/150‑1 (MQ=255)

 cAGATCGCGCGCACCTTCCATGCCCTGCTCTTTGGTGACAAGAATCAGTTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTTCCAg                                                                                                                                               <  1:2587207/150‑1 (MQ=255)

      cgcgcgCACCTTCCATGCCCTGCTCTTTGGTGACAAGAATCAGTTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTTCCAGCGCaa                                                                                                                                          <  1:2881023/150‑1 (MQ=255)

       gcgcgcACCTTCCATGCCCTGCTCTTTGGTGACAAGAATCAGTTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTTCCAGCGCAAt                                                                                                                                         <  2:4315085/150‑1 (MQ=255)

        cgcgcACCTTCCATGCCCTGCTCTTTGGTGACAAGAATCAGTTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTTCCAGCGCAATg                                                                                                                                        <  2:4275199/150‑1 (MQ=255)

             aCCTTCCATGCCCTGCTCTTTGGTGACAAGAATCAGTTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTTCCAGCGCAATGCCGgt                                                                                                                                   >  2:3583621/1‑150 (MQ=255)

              ccTTCCATGCCCTGCTCTTTGGTGACAAGAATCAGTTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTTCCAGCGCAATGCCGgtg                                                                                                                                  >  2:4058938/1‑150 (MQ=255)

                ttCCATGCCCTGCTCTTTGGTGACAAGAATCAGTTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTTCCAGCGCAATGCCGGTGtt                                                                                                                                >  1:1012546/1‑150 (MQ=255)

                  ccATGCCCTGCTCTTTGGTGACAAGAATCAGTTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTTCCAGCGCAATGCCGGTGTTAc                                                                                                                              >  2:631054/1‑150 (MQ=255)

                   cATGCCCTGCTCTTTGGTGACAAGAATCAGTTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTTCCAGCGCAATGCCGGTGTTAcc                                                                                                                             >  1:2458249/1‑150 (MQ=255)

                    aTGCCCTGCTCTTTGGTGACAAGAATCAGTTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTTCCAGCGCAATGCCGGTGTTACCa                                                                                                                            <  2:2520235/150‑1 (MQ=255)

                    aTGCCCTGCTCTTTGGTGACAAGAATCAGTTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTTCCAGCGCAATGCCGGTGTTACCa                                                                                                                            <  2:3749106/150‑1 (MQ=255)

                        ccTGCTCTTTGGTGACAAGAATCAGTTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTTCCAGCGCAATGCCGGTGTTACCACtgg                                                                                                                        >  1:1793304/1‑150 (MQ=255)

                         cTGCTCTTTGGTGACAAGAATCAGTTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTTCCAGCGCAATGCCGGTGTTACCACtggt                                                                                                                       <  1:1239079/150‑1 (MQ=255)

                          tGCTCTTTGGTGACAAGAATCAGTTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTTCCAGCGCAATGCCGGTGTTACCACtggtg                                                                                                                      <  1:3618171/150‑1 (MQ=255)

                             tctTTGGTGACAAGAATCAGTTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTTCCAGCGCAATGCCGGTGTTACCACTGGTGGCt                                                                                                                   <  2:3630676/150‑1 (MQ=255)

                              ctTTGGTGACAAGAATCAGTTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTTCCAGCGCAATGCCGGTGTTACCACTGGTGGCtt                                                                                                                  >  1:1100459/1‑150 (MQ=255)

                               tttGGTGACAAGAATCAGTTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTTCCAGCGCAATGCCGGTGTTACCACTGGTGGCTTc                                                                                                                 <  1:469682/150‑1 (MQ=255)

                                  ggTGACAAGAATCAGTTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTTCCAGCGCAATGCCGGTGTTACCACTGGTGGCTTCGAt                                                                                                              <  1:2277592/150‑1 (MQ=255)

                                  ggTGACAAGAATCAGTTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTTCCAGCGCAATGCCGGTGTTACCACTGGTGGCTTCGAt                                                                                                              >  1:1001627/1‑150 (MQ=255)

                                  ggTGACAAGAATCAGTTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTTCCAGCGCAATGCCGGTGTTACCACTGGTGGCTTCGAt                                                                                                              <  1:4297887/150‑1 (MQ=255)

                                   gTGACAAGAATCAGTTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTTCCAGCGCAATGCCGGTGTTACCACTGGTGGCTTCGAtt                                                                                                             <  2:2314815/150‑1 (MQ=255)

                                   gTGACAAGAATCAGTTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTTCCAGCGCAATGCCGGTGTTACCACTGGTGGCTTCGAtt                                                                                                             >  2:1391061/1‑150 (MQ=255)

                                    tGACAAGAATCAGTTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTTCCAGCGCAATGCCGGTGTTACCACTGGTGGCTTCGATTa                                                                                                            <  1:3218780/150‑1 (MQ=255)

                                     gACAAGAATCAGTTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTTCCAGCGCAATGCCGGTGTTACCACTGGTGGCTTCGATTaa                                                                                                           <  1:116826/150‑1 (MQ=255)

                                      aCAAGAATCAGTTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTTCCAGCGCAATGCCGGTGTTACCACTGGTGGCTTCGATTAAg                                                                                                          >  1:1531459/1‑150 (MQ=255)

                                       cAAGAATCAGTTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTTCCAGCGCAATGCCGGTGTTACCACTGGTGGCTTCGATTAAGa                                                                                                         >  1:2563461/1‑150 (MQ=255)

                                           aaTCAGTTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTTCCAGCGCAATGCCGGTGTTACCACTGGTGGCTTCGATTAAGACATc                                                                                                     <  1:999761/150‑1 (MQ=255)

                                             tCAGTTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTTCCAGCGCAATGCCGGTGTTACCACTGGTGGCTTCGATTAAGACATCAc                                                                                                   >  2:1776863/1‑150 (MQ=255)

                                               aGTTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTTCCAGCGCAATGCCGGTGTTACCACTGGTGGCTTCGATTAAGACATCAccc                                                                                                 >  1:3370042/1‑150 (MQ=255)

                                                    cGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTTCCAGCGCAATGCCGGTGTTACCACTGGTGGCTTCGATTAAGACATCACCCGGttt                                                                                            <  2:1574397/150‑1 (MQ=255)

                                                     gCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTTCCAGCGCAATGCCGGTGTTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTa                                                                                           >  1:1581209/1‑150 (MQ=255)

                                                     gCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTTCCAGCGCAATGCCGGTGTTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTa                                                                                           >  2:1014974/1‑150 (MQ=255)

                                                     gCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTTCCAGCGCAATGCCGGTGTTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTa                                                                                           >  1:1143924/1‑150 (MQ=255)

                                                      cACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTTCCAGCGCAATGCCGGTGTTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTaa                                                                                          >  1:1307512/1‑150 (MQ=255)

                                                       aCCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTTCCAGCGCAATGCCGGTGTTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAAt                                                                                         >  1:3467733/1‑150 (MQ=255)

                                                         cATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTTCCAGCGCAATGCCGGTGTTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAAttt                                                                                       <  2:1870286/150‑1 (MQ=255)

                                                          aTAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTTCCAGCGCAATGCCGGTGTTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTc                                                                                      >  1:482110/1‑150 (MQ=255)

                                                                aCGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTTCCAGCGCAATGCCGGTGTTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCcgcg                                                                                <  1:2223661/150‑1 (MQ=255)

                                                                   cATCGCCGCTCGACGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCGTTCAGCGCGGCAATCATTTCCAGCGCAATGCCGGTGTTACCACTGGTGGCTTCGATTACGACATCACCCGGGTTAATTTTACGGCGCtt                                                                             >  2:2588666/1‑150 (MQ=255)

                                                                    aTCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTTCCAGCGCAATGCCGGTGTTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCttt                                                                            >  1:3034692/1‑150 (MQ=255)

                                                                    aTCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTTCCAGCGCAATGCCGGTGTTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCttt                                                                            >  1:2036692/1‑150 (MQ=255)

                                                                       gccgcACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTTCCAGCGCAATGCCGGTGTTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTcc                                                                         <  1:870497/150‑1 (MQ=255)

                                                                       gccgcACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTTCCAGCGCAATGCCGGTGTTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTcc                                                                         <  2:4364471/150‑1 (MQ=255)

                                                                           ctcGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTTCCAGCGCAATGCCGGTGTTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCt                                                                     >  2:379092/3‑150 (MQ=255)

                                                                                cGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTTCCAGCGCAATGCCGGTGTTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTcgacg                                                                >  2:605954/1‑150 (MQ=255)

                                                                                  ttCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTTCCAGCGCAATGCCGGTGTTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGat                                                              >  1:2310212/1‑150 (MQ=255)

                                                                                  ttCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTTCCAGCGCAATGCCGGTGTTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGat                                                              <  2:1267242/150‑1 (MQ=255)

                                                                                   tCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTTCCAGCGCAATGCCGGTGTTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGatc                                                             <  1:2439230/150‑1 (MQ=255)

                                                                                   tCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTTCCAGCGCAATGCCGGTGTTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGatc                                                             <  2:11907/150‑1 (MQ=255)

                                                                                    ccTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTTCCAGCGCAATGCCGGTGTTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGatca                                                            <  1:2798126/150‑1 (MQ=255)

                                                                                       ggCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTTCCAGCTCAATGCCGGTGTTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCg                                                         >  1:3560680/1‑150 (MQ=255)

                                                                                        gCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTTCCAGCGCAATGCCGGTGTTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGa                                                        >  2:2142408/1‑150 (MQ=255)

                                                                                          tCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTTCCAGCGCAATGCCGGTGTTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGaaa                                                      <  1:1896769/150‑1 (MQ=255)

                                                                                          tCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTTCCAGCGCAATGCCGGTGTTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGaaa                                                      <  1:4021044/150‑1 (MQ=255)

                                                                                          tCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTTCCAGCGCAATGCCGGTGTTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGaaa                                                      >  1:791925/1‑150 (MQ=255)

                                                                                          tCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTTCCAGCGCAATGCCGGTGTTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGaaa                                                      >  1:3010241/1‑150 (MQ=255)

                                                                                              gttgtCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTTCCAGCGCAATGCCGGTGTTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGc                                                  >  1:2106515/1‑150 (MQ=255)

                                                                                              gttgtCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTTCCAGCGCAATGCCGGTGTTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGc                                                  >  2:3083438/1‑150 (MQ=255)

                                                                                               ttgtCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTTCCAGCGCAATGCCGGTGTTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGcc                                                 <  2:4121314/150‑1 (MQ=255)

                                                                                               ttgtCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTTCCAGCGCAATGCCGGTGTTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGcc                                                 <  1:936640/150‑1 (MQ=255)

                                                                                                tgtCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTTCCAGCGCAATGCCGGTGTTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCg                                                >  2:754965/1‑150 (MQ=255)

                                                                                                 gtCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTTCCAGCGCAATGCCGGTGTTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGc                                               >  1:3931448/1‑150 (MQ=255)

                                                                                                 gtCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTTCCAGCGCAATGCCGGTGTTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGc                                               <  2:3229072/150‑1 (MQ=255)

                                                                                                   cGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTTCCAGCGCAATGCCGGTGTTCCCACAGGTGGCTTCGATTAGGACACCACCCGGTTTAATTTCCCCGGGCTTTTCCGCATCGACGATCATCGAAAGTGCCGCAc                                             <  2:1890329/150‑1 (MQ=255)

                                                                                                      gCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTTCCAGCGCAATGCCGGTGTTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGAt                                          <  1:1382430/150‑1 (MQ=255)

                                                                                                        aTCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTTCCAGCGCAATGCCGGTGTTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGa                                                        >  1:909187/1‑134 (MQ=255)

                                                                                                        aTCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTTCCAGCGCAATGCCGGTGTTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGa                                                        <  2:909187/134‑1 (MQ=255)

                                                                                                        aTCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTTCCAGCGCAATGCCGGTGTTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCt                                        >  1:3851346/1‑150 (MQ=255)

                                                                                                        aTCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTTCCAGCGCAATGCCGGTGTTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCt                                        >  1:1520851/1‑150 (MQ=255)

                                                                                                         tCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTTCCAGCGCAATGCCGGTGTTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCtt                                       <  1:4117587/150‑1 (MQ=255)

                                                                                                             cAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTTCCAGCGCAATGCCGGTGTTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCAc                                   >  2:3300217/1‑150 (MQ=255)

                                                                                                             cAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTTCCAGCGCAATGCCGGTGTTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCAc                                   >  1:1688047/1‑150 (MQ=255)

                                                                                                              aaTTTCATGCGATAGCCTTTCAGCGCGGCAATCATTTCCAGCGCAATGCCGGTGTTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCAcc                                  >  1:3770522/1‑150 (MQ=255)

                                                                                                                   cATGCGATAGCCTTTCAGCGCGGCAATCATTTCCAGCGCAATGCCGGTGTTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAAcc                             >  1:3181089/1‑150 (MQ=255)

                                                                                                                   cATGCGATAGCCTTTCAGCGCGGCAATCATTTCCAGCGCAATGCCGGTGTTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAAcc                             >  1:1620499/1‑150 (MQ=255)

                                                                                                                   cATGCGATAGCCTTTCAGCGCGGCAATCATTTCCAGCGCAATGCCGGTGTTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAAcc                             <  2:2719297/150‑1 (MQ=255)

                                                                                                                       cGATAGCCTTTCAGCGCGGCAATCATTTCCAGCGCAATGCCGGTGTTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGcc                         <  2:1669042/150‑1 (MQ=255)

                                                                                                                        gATAGCCTTTCAGCGCGGCAATCATTTCCAGCGCAATGCCGGTGTTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCg                        >  1:4202657/1‑150 (MQ=255)

                                                                                                                          tAGCCTTTCAGCGCGGCAATCATTTCCAGCGCAATGCCGGTGTTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCggg                      >  1:3110176/1‑150 (MQ=255)

                                                                                                                           aGCCTTTCAGCGCGGCAATCATTTCCAGCGCAATGCCGGTGTTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGt                     >  2:1480703/1‑150 (MQ=255)

                                                                                                                            gCCTTTCAGCGCGGCAATCATTTCCAGCGCAATGCCGGTGTTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGtt                    >  2:3855818/1‑150 (MQ=255)

                                                                                                                             ccTTTCAGCGCGGCAATCATTTCCAGCGCAATGCCGGTGTTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGTTa                   >  1:3913583/1‑150 (MQ=255)

                                                                                                                             ccTTTCAGCGCGGCAATCATTTCCAGCGCAATGCCGGTGTTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGTTa                   >  1:4032728/1‑150 (MQ=255)

                                                                                                                             ccTTTCAGCGCGGCAATCATTTCCAGCGCAATGCCGGTGTTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGTTa                   >  2:1500242/1‑150 (MQ=255)

                                                                                                                              cTTTCAGCGCGGCAATCATTTCCAGCGCAATGCCGGTGTTACCACTGGTGGCTTCGATTAAGACATCACCCGGtt                                                                                             <  2:1144122/75‑1 (MQ=255)

                                                                                                                               tttCAGCGCGGCAATCATTTCCAGCGCAATGCCGGTGTTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGTTAtt                 >  2:3481816/1‑150 (MQ=255)

                                                                                                                               tttCAGCGCGGCAATCATTTCCAGCGCAATGCCGGTGTTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGTTAtt                 <  1:3694119/150‑1 (MQ=255)

                                                                                                                                ttCAGCGCGGCAATCATTTCCAGCGCAATGCCGGTGTTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGTTATTg                >  2:3412270/1‑150 (MQ=255)

                                                                                                                                ttCAGCGCGGCAATCATTTCCAGCGCAATGCCGGTGTTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGTTATTg                <  2:3353516/150‑1 (MQ=255)

                                                                                                                                    gcgcGGCAATCATTTCCAGCGCAATGCCGGTGTTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGTTATTGCCtt            <  2:299426/150‑1 (MQ=255)

                                                                                                                                         gCAATCATTTCCAGCGCAATGCCGGTGTTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGTTATTGCCTTCCAGt       <  2:3985503/150‑1 (MQ=255)

                                                                                                                                          cAATCATTTCCAGCGCAATGCCGGTGTTACCACTGGTGGCTTCGATTAAGGCATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGTTATTGCCTTCCAGtt      <  1:665869/150‑1 (MQ=255)

                                                                                                                                          cAATCATTTCCAGCGCAATGCCGGTGTTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGTTATTGCCTTCCAGtt      <  1:3940618/150‑1 (MQ=255)

                                                                                                                                           aaTCATTTCCAGCGCAATGCCGGTGTTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGTTATTGCCTTCCAGttt     >  2:943483/1‑150 (MQ=255)

                                                                                                                                            aTCATTTCCAGCGCAATGCCGGTGTTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGTTATTGCCTTCCAGtttt    >  2:2044714/1‑150 (MQ=255)

                                                                                                                                              cATTTCCAGCGCAATGCCGGTGTTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGTTATTGCCTTCCAGTTTTaa  <  2:2970364/150‑1 (MQ=255)

                                                                                                                                              cATTTCCAGCGCAATGCCGGTGTTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGTTATTGCCTTCCAGTTTTaa  >  2:2791762/1‑150 (MQ=255)

CCAGATCGCGCGCACCTTCCATGCCCTGCTCTTTGGTGACAAGAATCAGTTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTGCCAGCGCAATGCCGGTGTTACCACTGGTGGCTTCGATTAAGACATCACCCGGTTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGTTATTGCCTTCCAGTTTTAA  >  AP012306/2091625‑2091916

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`