Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP012306800,6750AC28.8% 540.0 / 35.6 243T192P (ACC→CCC) lolAchaperone for lipoproteins
Reads supporting (aligned to +/- strand):  ref base A (136/37);  new base C (0/70);  total (136/107)
Fisher's exact test for biased strand distribution p-value = 5.71e-34
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 304 total aligned reads displayed.

GCAATGGCAATCTGAAGCAGTTCACCATTAACGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAGTATATCGGCCAGCAAC  >  AP012306/800526‑800823
                                                                                                                                                     |                                                                                                                                                    
gCAATGGCAATCTGAAGCAGTTCACCATTAACGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCa                                                                                                                                                      <  1:2471507/150‑1 (MQ=255)
  aaTGGCAATCTGAAGCAGTTCACCATTAACGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCAcc                                                                                                                                                    >  2:461689/1‑150 (MQ=255)
  aaTGGCAATCTGAAGCAGTTCACCATTAACGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCAcc                                                                                                                                                    >  2:3422398/1‑150 (MQ=255)
  aaTGGCAATCTGAAGCAGTTCACCATTAACGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCAcc                                                                                                                                                    <  1:2971952/150‑1 (MQ=255)
     ggCAATCTGAAGCAGTTCACCATTAACGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCccg                                                                                                                                                 <  1:1002674/150‑1 (MQ=255)
       cAATCTGAAGCAGTTCACCATTAACGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCccgcc                                                                                                                                               >  1:1092835/1‑150 (MQ=255)
        aaTCTGAAGCAGTGCACCATTAACGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCCCCccgccg                                                                                                                                              <  2:2681258/150‑1 (MQ=255)
            tGAAGCAGTTCACCATTAACGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAg                                                                                                                                          >  1:3956/1‑150 (MQ=255)
             gAAGCAGTTCACCATTAACGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAgg                                                                                                                                         >  2:2404027/1‑150 (MQ=255)
             gAAGCAGTTCACCATTAACGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAgg                                                                                                                                         >  1:786677/1‑150 (MQ=255)
               aGCAGTTCACCATTAACGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCg                                                                                                                                       >  2:62347/1‑150 (MQ=255)
               aGCAGTTCACCATTAACGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCg                                                                                                                                       >  2:4049458/1‑150 (MQ=255)
                  aGTTCACCATTAACGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCa                                                                                                                                    >  1:3710621/1‑150 (MQ=255)
                  aGTTCACCATTAACGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCa                                                                                                                                    >  1:3639580/1‑150 (MQ=255)
                    ttCACCATTAACGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCATTTATCAACTGAAATCCCAGCAAAAGGGGGCTGTGGATGCAGCGAAATTTCCCTTCCCCCCGCCGCAAGGCGTCACg                                                                                                                                  <  2:1213299/150‑1 (MQ=255)
                    ttCACCATTAACGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCCCCCCGCCGCAAGGCGTCACg                                                                                                                                  <  1:206425/150‑1 (MQ=255)
                             aaCGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAgatgat                                                                                                                         >  1:3225522/1‑150 (MQ=255)
                             aaCGGGGGACGTGATGCCACAACCCATAAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCATTTATCAACTGAAACCCCAGAAAAATGGGGCTGTGGATGCAGCGAAATTTCCCTTCCCCCCGCCGCAAGGCGTCACGGTAgatgat                                                                                                                         <  2:2140967/150‑1 (MQ=255)
                               cGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCa                                                                                                                       >  2:2343030/1‑150 (MQ=255)
                                     aCGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTaa                                                                                                                 >  2:2063071/1‑150 (MQ=255)
                                       gTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAACCCCAGAAAAAGGGGGCTGTGGATGCAGGAAAATTTACCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGt                                                                                                               <  1:930864/150‑1 (MQ=255)
                                        tGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTa                                                                                                              >  2:3624893/1‑150 (MQ=255)
                                         gATGACACAACCCATCGGTTAAGCGGGGTGGAGAAGAACGATCAGCGCACCGGTTATCAACGGAATTCCCAGAAAAGGGGGGCTGTGGATGCAGCGAATTTTCCCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTag                                                                                                             <  2:1028286/150‑1 (MQ=255)
                                          aTGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTaga                                                                                                            >  2:4115638/1‑150 (MQ=255)
                                          aTGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTaga                                                                                                            >  1:3377899/1‑150 (MQ=255)
                                           tGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAAGGGGGCTGTGGATGCAGCGAAATTTACCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTagag                                                                                                           <  2:3902183/150‑1 (MQ=255)
                                              cacaATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCa                                                                                                        <  1:551553/150‑1 (MQ=255)
                                                caATCCATCAGTTTGGCGGGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAAGGGGGTTGTGGTTGGAGCGAAATTTACCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCAcc                                                                                                      <  2:1125709/150‑1 (MQ=255)
                                                caATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCAcc                                                                                                      >  2:4174639/1‑150 (MQ=255)
                                                  aTCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCtg                                                                                                    <  1:429033/150‑1 (MQ=255)
                                                  aTCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCtg                                                                                                    >  1:2372128/1‑150 (MQ=255)
                                                  aTCCATCAGTTTAGCGCGGGGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAACCCCAGCAAAAGGGGGCTGTGGATGCAGCGAAATTTCCCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCtg                                                                                                    <  2:1967763/150‑1 (MQ=255)
                                                   tCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCtga                                                                                                   >  1:2816668/1‑150 (MQ=255)
                                                     cATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCtgagt                                                                                                 <  1:3342091/150‑1 (MQ=255)
                                                     cATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAACCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCtgagt                                                                                                 <  1:4116119/150‑1 (MQ=255)
                                                      aTCAGTTTCGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCTTCACGGTAGATGATCAACGTAAGTAGAGGCACCtgagtg                                                                                                >  2:3054178/1‑150 (MQ=255)
                                                        cAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTCCCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCtgagtgag                                                                                              <  1:2510379/150‑1 (MQ=255)
                                                         aGTTTAGCGCGGTGGAGCAGGACGATCAGGGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGGGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGc                                                                                             <  1:1158361/150‑1 (MQ=255)
                                                         aGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGc                                                                                             >  1:3505145/1‑150 (MQ=255)
                                                          gTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCa                                                                                            >  1:1668605/1‑150 (MQ=255)
                                                              aGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTCCCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCt                                                                                        <  2:4261839/150‑1 (MQ=255)
                                                               gcgcGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGAAAAAGGGGGCTGTGGATGCAGCAAAATTTACCTCCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTg                                                                                       <  2:3159064/150‑1 (MQ=255)
                                                                cgcgGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAACCCCAGCAAAAGGGGGCTGTGGATGCAGCGAAATTTCCCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGt                                                                                      <  2:2445724/150‑1 (MQ=255)
                                                                 ggggTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAACTCCCAGAAAAATGGGGCTGTGGATGCAGCGAAATTTACCTCCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTc                                                                                     <  2:2248418/148‑1 (MQ=255)
                                                                   ggTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAAATTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGc                                                                                   >  2:1668729/1‑150 (MQ=255)
                                                                    gTGGAGCAGGACGATCAGCGCAGCGGTTATCAACTGAAACCCCAGCAAAATGGGGCTGTGGATGCAGCAAAATTTACCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCt                                                                                  <  2:1914178/150‑1 (MQ=255)
                                                                    gTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCt                                                                                  >  1:913345/1‑150 (MQ=255)
                                                                     tGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTc                                                                                 >  1:1736657/1‑150 (MQ=255)
                                                                        aGCAGGACGATCAGCGCAGCAGTTATCAACTGAAACCCCAGCAAAAGGGGGCTGTGGATGCAGCGAAATTTCCCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGAt                                                                              <  2:1785349/150‑1 (MQ=255)
                                                                         gCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGAtt                                                                             <  1:4020758/150‑1 (MQ=255)
                                                                          cAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTTTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGAttt                                                                            >  1:3454744/1‑150 (MQ=255)
                                                                          cAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGAttt                                                                            >  1:3165637/1‑150 (MQ=255)
                                                                             gACGATCAGCGCAGCAGTTATCAACTAAAATCCCAGAAAAATGGGGTTGTGGATGCAGCGAAATTTCCCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTc                                                                         <  2:2591817/150‑1 (MQ=255)
                                                                               cGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCgg                                                                       >  2:4000777/1‑150 (MQ=255)
                                                                                   cAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGataa                                                                   >  1:2554579/1‑150 (MQ=255)
                                                                                   cAGCGCAGCAGTTATCAACTAAAACCCCAGAAAAATGGGGCTGGGGATGAAGCAAAATTTACCTCCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGataa                                                                   <  2:3359851/150‑1 (MQ=255)
                                                                                    aGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGataat                                                                  >  2:3544085/1‑150 (MQ=255)
                                                                                    aGCGCAGCAGTTATCAACTGAAATCCCAGCAAAAGGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGataat                                                                  <  1:1783962/150‑1 (MQ=255)
                                                                                     gcgcAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGAGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGataata                                                                 <  1:1302048/150‑1 (MQ=255)
                                                                                      cgcAGCAGTTATCAACTGAAACCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATAc                                                                <  2:734002/150‑1 (MQ=255)
                                                                                      cgcAGCAGTTATCAACTAAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATAc                                                                <  2:2905586/150‑1 (MQ=255)
                                                                                       gcagcaGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACt                                                               >  2:3869022/1‑150 (MQ=255)
                                                                                          gcagTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACtttt                                                            >  2:2185553/1‑150 (MQ=255)
                                                                                          gcagTTAAAAACTGAAACCCCAGAAAAATGGGGCTGTGGATGCAGCAAAATTTACCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACtttt                                                            <  2:1359162/150‑1 (MQ=255)
                                                                                            agTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCa                                                          >  2:2756517/1‑150 (MQ=255)
                                                                                               tATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAAcc                                                       >  1:694940/1‑150 (MQ=255)
                                                                                                  cAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACctct                                                    >  2:707490/1‑150 (MQ=255)
                                                                                                   aaCTAAAACCCCAGCAAAAGGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTg                                                   <  2:1530525/150‑1 (MQ=255)
                                                                                                    aCTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTgg                                                  >  2:814819/1‑150 (MQ=255)
                                                                                                    aCTGAAATCCCAGCAAAAGGGGGGTGTGGATGAAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTgg                                                  <  2:3985049/150‑1 (MQ=255)
                                                                                                      tGAAATCCCAGCAAATTGGGGCTGTGGATGCAGCGAAATTTACCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGcc                                                <  2:3558500/150‑1 (MQ=255)
                                                                                                         aaTCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCcgcg                                             >  2:105395/1‑150 (MQ=255)
                                                                                                             ccAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTa                                         >  1:423386/1‑150 (MQ=255)
                                                                                                              cAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTAt                                        >  2:1310958/1‑150 (MQ=255)
                                                                                                              cAGCAAAATGGGGCTGGGGATGCAGCAAAATTTCCCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTAt                                        <  2:1749342/150‑1 (MQ=255)
                                                                                                                  aaaaTGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCgg                                    <  1:2217226/150‑1 (MQ=255)
                                                                                                                  aaaaTGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCgg                                    <  1:62347/150‑1 (MQ=255)
                                                                                                                   aaaGGGGGTTGGGGATGCAGCGAAATTTACCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGc                                   <  2:3121265/150‑1 (MQ=255)
                                                                                                                        gggCTGTGGATGCAGCGAAATTTCCCTCCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGaa                              <  1:3130532/150‑1 (MQ=255)
                                                                                                                         ggttgtgGATGCAGCGAATTTTACCTCCGCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGaaa                             <  2:2974772/147‑1 (MQ=255)
                                                                                                                            tgtgGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAAtt                          >  2:3947856/1‑150 (MQ=255)
                                                                                                                            tgtgCATGCAGAGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGGGCGTATGCGGCCAGAAAAtt                          <  1:2933065/150‑1 (MQ=255)
                                                                                                                            tggggttGCAGCAAAATTTCCCTCCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAAtt                          <  2:252798/144‑1 (MQ=255)
                                                                                                                             gtgGATGCAGCGAAATTTCCCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAAttt                         <  1:1537306/150‑1 (MQ=255)
                                                                                                                                gATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGc                      >  2:1481949/1‑150 (MQ=255)
                                                                                                                                gATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGc                      >  2:2010520/1‑150 (MQ=255)
                                                                                                                                 aggcagcaAAATTTCCCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGca                     <  2:3805853/148‑1 (MQ=255)
                                                                                                                                   gCAGCGAAATTTCCCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTGGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGcaca                   <  2:3257427/150‑1 (MQ=255)
                                                                                                                                      gCGAAATTTACCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAGta                <  2:532751/150‑1 (MQ=255)
                                                                                                                                        gAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAGtata              <  1:3590902/150‑1 (MQ=255)
                                                                                                                                        aaaaTTTACCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAGtata              <  2:1088987/149‑1 (MQ=255)
                                                                                                                                            tttCCCTCCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAGTATATCgg          <  1:991074/150‑1 (MQ=255)
                                                                                                                                            tttACCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAGTATATCgg          <  1:2094500/150‑1 (MQ=255)
                                                                                                                                              tACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAGTATATCGGcc        <  1:1246695/150‑1 (MQ=255)
                                                                                                                                              tACCTCCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAGTATATCGGcc        <  2:3743720/150‑1 (MQ=255)
                                                                                                                                               aCCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAGTATATCGGCCa       >  1:2689947/1‑150 (MQ=255)
                                                                                                                                                ccTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAGTATATCGGCCAg      <  1:1199629/150‑1 (MQ=255)
                                                                                                                                                 cTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAGTATATCGGCCAGc     <  1:3412574/150‑1 (MQ=255)
                                                                                                                                                 cTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAGTATATCGGCCAGc     <  1:4074947/150‑1 (MQ=255)
                                                                                                                                                 cTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAGTATATCGGCCAGc     >  1:2789478/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GCAATGGCAATCTGAAGCAGTTCACCATTAACGTGGGACGTGATGGCACAATCCATCAGTTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAGTATATCGGCCAGCAAC  >  AP012306/800526‑800823

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑