Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123061,226,5570AG24.5% 462.7 / 87.2 204G55G (GGA→GGG) ydcUpredicted spermidine/putrescine transporter subunit
Reads supporting (aligned to +/- strand):  ref base A (19/135);  new base G (50/0);  total (69/135)
Fisher's exact test for biased strand distribution p-value = 3.28e-32
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 229 total aligned reads displayed.

TGCAATCACCTTCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTA‑TACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTG‑TTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCAGCGCCATTCTGGC  >  AP012306/1226409‑1226706
                                                                                                                                                    |                                                                                                                                                       
 gCAATCACCTTCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGAtt                                                                                                                                                       <  2:2438353/150‑1 (MQ=255)
 gCAATCACCTTCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGAtt                                                                                                                                                       <  1:2384243/150‑1 (MQ=255)
  cAATCACCTTCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGttt                                                                                                                                                      >  1:3959131/1‑150 (MQ=255)
  cAATCACCTTCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGAttt                                                                                                                                                      <  2:4006465/150‑1 (MQ=255)
     tCACCTTCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTta‑t                                                                                                                                                  <  1:3079856/150‑1 (MQ=255)
        ccTTCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTA‑TACt                                                                                                                                               <  1:1900969/150‑1 (MQ=255)
        ccTTCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGAGTTTTT‑TACt                                                                                                                                               >  1:4235097/1‑150 (MQ=255)
         cTTCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTA‑TACtt                                                                                                                                              <  2:1840655/150‑1 (MQ=255)
         cTTCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTA‑TACtt                                                                                                                                              <  2:2378535/150‑1 (MQ=255)
          ttCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTA‑TACttt                                                                                                                                             <  1:3121193/150‑1 (MQ=255)
           tCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTA‑TACtttt                                                                                                                                            <  1:1192591/150‑1 (MQ=255)
            cACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTT‑TAAttttt                                                                                                                                           >  1:988737/1‑149 (MQ=255)
             aCGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTA‑TACTTTTGa                                                                                                                                          <  1:3237869/150‑1 (MQ=255)
             aCGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTA‑TACTTTTGa                                                                                                                                          <  1:231848/150‑1 (MQ=255)
              cGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTA‑TACTTTTGAc                                                                                                                                         <  2:2114003/150‑1 (MQ=255)
                  cAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTA‑TACTTTTTACGAtt                                                                                                                                     >  1:2542030/1‑150 (MQ=255)
                   aGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTA‑TACTTTTGACGAttt                                                                                                                                    <  1:2722276/150‑1 (MQ=255)
                    ggTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTA‑TACTTTTGACGATTTc                                                                                                                                   <  1:599537/150‑1 (MQ=255)
                      tCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTA‑TACTTTTGACGATTTCAc                                                                                                                                 <  1:2116436/150‑1 (MQ=255)
                       cTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTT‑TTATTTTTACGATTTCAcc                                                                                                                                >  2:1599536/1‑150 (MQ=255)
                       cTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTA‑TACTTTTGACGATTTCAcc                                                                                                                                <  1:3441768/150‑1 (MQ=255)
                         gggTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTA‑TACTTTTTACGATTTCACCAt                                                                                                                              >  1:4178424/1‑150 (MQ=255)
                           gTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTA‑TACTTTTGACGATTTCACCATGt                                                                                                                            <  1:2572912/150‑1 (MQ=255)
                             aaGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTA‑TACTTTTGACGATTTCACCATGTCg                                                                                                                          <  1:2587490/150‑1 (MQ=255)
                             aaGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTA‑TACTTTTGACGATTTCACCATGTCg                                                                                                                          <  2:3451399/150‑1 (MQ=255)
                              aGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTA‑TACTTTTGACGATTTCACCATGTCgg                                                                                                                         <  2:7944/150‑1 (MQ=255)
                               ggTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGATTTT‑TACTTTTTTCGATTTCACCAATTCggg                                                                                                                        >  2:2616953/1‑149 (MQ=255)
                                  gtCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTA‑TACTTTTGACGATTTCACCATGTCGGTAAc                                                                                                                     <  1:3210232/150‑1 (MQ=255)
                                    ccGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTT‑TTTTTTTTTCGATTTTACCCTTTCGGTTACGc                                                                                                                   >  2:1236956/1‑150 (MQ=255)
                                     cGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTTGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTT‑TACTTTTGACGATTTCACCCTGCCGGGAAcccc                                                                                                                  >  1:1104651/1‑150 (MQ=255)
                                     cGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTT‑TTCTTTTTACGATTTTACCATGTCGGTAACGcc                                                                                                                  >  1:3671126/1‑150 (MQ=255)
                                     cGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTA‑TACTTTTGACGATTTCACCATGTCGGTAACGcc                                                                                                                  <  2:1442315/150‑1 (MQ=255)
                                         ttcttcTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTA‑TACTTTTGACGATTTCACCATGTCGGTAACGCCGGaa                                                                                                              <  2:4128631/150‑1 (MQ=255)
                                           cttctGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTT‑TTCTTTTTTCGATTTTACCCTGTCGGTAACGCCGGGACt                                                                                                            >  2:802403/1‑150 (MQ=255)
                                              ctGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTA‑TACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGAc                                                                                                         <  2:1539021/150‑1 (MQ=255)
                                              ctGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCACTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTA‑TACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGAc                                                                                                         <  2:383485/150‑1 (MQ=255)
                                               tGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTA‑TACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACg                                                                                                        <  2:2061800/150‑1 (MQ=255)
                                               tGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTA‑TACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACg                                                                                                        <  1:1626639/150‑1 (MQ=255)
                                               tGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTA‑TACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACg                                                                                                        <  1:4022449/150‑1 (MQ=255)
                                                  cATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTA‑TACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTg                                                                                                     <  1:3923754/150‑1 (MQ=255)
                                                    tAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTA‑TACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGc                                                                                                   <  1:427840/150‑1 (MQ=255)
                                                      aTCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTT‑TACTTTTTACGATTTTACCATGTCGGTAACGCCGGGAATGACGCTGGCgg                                                                                                 >  2:550526/1‑149 (MQ=255)
                                                          ggggctggggctGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTA‑TACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAatat                                                                                             <  2:3573818/150‑1 (MQ=255)
                                                          ggggctggggctGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTA‑TACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAatat                                                                                             <  1:1636331/150‑1 (MQ=255)
                                                           gggctggggctgTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTA‑TACTTTTTACGATTTCACCATGTCGGTAAAGCCCGAACTGACGCTGGCGAatatt                                                                                            >  1:1082537/1‑149 (MQ=255)
                                                            ggctggggctgTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTA‑TACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATcc                                                                                           <  2:169692/150‑1 (MQ=255)
                                                              ctggggctgTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTA‑TACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGt                                                                                         <  1:1886606/150‑1 (MQ=255)
                                                               tggggctgTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTA‑TACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTg                                                                                        <  1:1117508/150‑1 (MQ=255)
                                                               tggggctgTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTA‑TACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTg                                                                                        <  1:3225631/150‑1 (MQ=255)
                                                                ggggctgTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTA‑TACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTgc                                                                                       <  1:173146/150‑1 (MQ=255)
                                                                ggggctgTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTA‑TACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTgc                                                                                       <  1:1362158/150‑1 (MQ=255)
                                                                     tgTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTA‑TACTTTTTACGAATTTACCAAGTCGGTAACGCCCGGACTGACGCTGGCGAATATCCGTGGGCCG‑t                                                                                 >  1:736581/1‑150 (MQ=255)
                                                                        ttttACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTA‑TACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTG‑TTTa                                                                              <  2:3597965/150‑1 (MQ=255)
                                                                        ttttACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTA‑TACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTG‑TTTa                                                                              <  1:3839835/150‑1 (MQ=255)
                                                                           tACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTA‑TATTTTTTTCGATTTCACCATTTCGGGAACACCGGAACTGACGCTGGCGAATATTCGTGCGCTG‑CTTTAtt                                                                           >  2:4047112/1‑149 (MQ=255)
                                                                               gctgctTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTA‑TACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTG‑TTTAATCCGGc                                                                       <  2:2679991/150‑1 (MQ=255)
                                                                                    ttGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTA‑TACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTG‑TTTAATCCGGCGAAtt                                                                  <  2:2301355/150‑1 (MQ=255)
                                                                                    ttGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTA‑TACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTG‑TTTAATCCGGCGAAtt                                                                  <  1:3046999/150‑1 (MQ=255)
                                                                                     tGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTATTACTTTT‑AAGATTTCACCATGTTGGTTACGCCCGAAATTATGCTGGGGAAAATCCTTGTCCTG‑TTTTATCTGGCgtagtt                                                                 >  2:1159606/1‑145 (MQ=255)
                                                                                        cccGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTA‑TACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTG‑TTTAATCCGGCGAATTACGa                                                              <  1:1007174/150‑1 (MQ=255)
                                                                                        cccGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTA‑TACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTG‑TTTAATCCGGCGAATTACGa                                                              <  2:1314048/150‑1 (MQ=255)
                                                                                         ccGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTA‑TACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTG‑TTTAATCCGGCGAATTACGAc                                                             <  1:87477/150‑1 (MQ=255)
                                                                                           gCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTA‑TACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTG‑TTTAATCCGGCGAATTACGAcat                                                           <  2:3210763/150‑1 (MQ=255)
                                                                                              aaTGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTT‑TAATTTTTTCGATTTTACCATGTCGGTAAAGCCGGAACTGACGCTGGCGAATATCCGTGCGCTG‑TTTAATCCGGCGAATTACGAcatcat                                                        >  2:1217199/1‑150 (MQ=255)
                                                                                              aaTGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTA‑TACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTG‑TTTAATCCGGCGAATTACGAcatcat                                                        <  2:1763777/150‑1 (MQ=255)
                                                                                              aaTGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTA‑TACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTG‑TTTAATCCGGCGAATTACGAcatcat                                                        <  2:1934786/150‑1 (MQ=255)
                                                                                               aTGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTA‑TACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTG‑TTTAATCCGGCGAATTACGACATCAtt                                                       <  1:3268030/150‑1 (MQ=255)
                                                                                                 gtgGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTT‑TATTTTTTACGATTTTACCCCTTCGGTTACGCCGGGACTGAAGCTGGCGAATATCCCGGCGCTG‑TTTTATTCGGCGGATTACGACATCATtct                                                     >  2:2253432/1‑150 (MQ=255)
                                                                                                  tgGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTA‑TACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTG‑TTTAATCCGGCGAATTACGACATCATtctc                                                    <  1:2682455/150‑1 (MQ=255)
                                                                                                  tgGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTA‑TACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTG‑TTTAATCCGGCGAATTACGACATCATtctc                                                    <  2:2483773/150‑1 (MQ=255)
                                                                                                    gTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTT‑TAATTTTTACGATTTTACCCTGTCGGTAAAGCCGGGACCGACGCCGGCGGAAATCCGTGCGCTG‑TTTTATCCGGGGAATTACGACATCATTCTccc                                                  >  2:4274612/1‑149 (MQ=255)
                                                                                                     tttGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTA‑TACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTG‑TTTAATCCGGCGAATTACGACATCATTCTCCGc                                                 <  1:3207206/150‑1 (MQ=255)
                                                                                                      ttGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTT‑TACTTTTTACGGTTTCACCATGTCGGGAAACCCCGGACTGAAGCCGGCGCATATCCCCGCGCCG‑TTTTATCCCGCGAATTACCACATCATTTTCCCCa                                                >  2:688097/1‑150 (MQ=255)
                                                                                                      ttGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTA‑TACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTG‑TTTAATCCGGCGAATTACGACATCATTCTCCGCa                                                >  1:3760557/1‑150 (MQ=255)
                                                                                                      ttGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTA‑TACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTG‑TTTAATCCGGCGAATTACGACATCATTCTCCGCa                                                <  1:3610106/150‑1 (MQ=255)
                                                                                                        ggCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTT‑TACTTTTTACGATTTTACCATGTCGGTAACGCCGGAAATGACGCTGGCGAATATCCGTGCGCTG‑TTTTATCCGGCGAATTACGACATCATTCTCCGCACg                                              >  2:844401/1‑150 (MQ=255)
                                                                                                          cATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTT‑TAATTTTTTCGATTTCACCATGTCGGGAACGCCGGGACTGACGCCGGCGAATATCCGTGCGCTG‑TTTTATTCGGCGAATTACGACATCATTTTCCCCACGCt                                            >  2:4211114/1‑150 (MQ=255)
                                                                                                           aTTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTA‑TACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTG‑TTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTg                                           <  2:3119970/150‑1 (MQ=255)
                                                                                                             tGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTA‑TACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTG‑TTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGAc                                         <  2:887805/150‑1 (MQ=255)
                                                                                                              gTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTT‑TACTTTTTGCGATTTTACCATGTCGGGAAAGCCCGGACTGACGCTGGCGAATATCCGTGCGCTG‑TTTTATCCGGCGAAATACGGCATCATTCTCCGCCCGCTGACt                                        >  2:2223127/1‑150 (MQ=255)
                                                                                                               tCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTT‑TACTTTTTACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTG‑TTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTa                                       >  2:524709/1‑150 (MQ=255)
                                                                                                                  aTTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTT‑TACTTTTGACGATTTCAACCTGTCGGTGACGCCGGAACTTATGCTGGCGACAATACGTGGGCTGCTTTATTCCGG‑GAATTAAGACATCATTTTCCGCACGCTGTCTAttg                                    >  2:2785238/1‑148 (MQ=255)
                                                                                                                  aTTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTA‑TACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTG‑TTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATgg                                    <  1:2083295/150‑1 (MQ=255)
                                                                                                                   tttCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTA‑TACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTG‑TTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGc                                   <  1:550526/150‑1 (MQ=255)
                                                                                                                     tCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTA‑TACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTG‑TTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCtg                                 <  1:3932318/150‑1 (MQ=255)
                                                                                                                      cGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTA‑TACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTG‑TTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCtgt                                <  1:682903/150‑1 (MQ=255)
                                                                                                                           cGCTGCTGACACTGTTATGGCAGGGATTTTA‑TACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTG‑TTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAg                           <  2:2947547/150‑1 (MQ=255)
                                                                                                                            gctgctGACACTGTTATGGCAGGGGTTTTT‑TACTTTTTTCGCTTTTCCCATGTCGGTAACGCCGGAACTGACCCTGGCGAATATTCGTGCGCTG‑TTTTATCCGGCGAATTACGACATCAATTTCCGCACGCTGACTATGGCTGGGGCCGt                          >  2:1096370/1‑150 (MQ=255)
                                                                                                                            gctgctGACACTGTTATGGCAGGGATTTTA‑TACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTG‑TTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTTACTATGGCTGTGGCAGt                          <  2:575790/150‑1 (MQ=255)
                                                                                                                            gctgctGACACTGTTATGGCAGGGATTTTA‑TACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTG‑TTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGt                          >  1:2263253/1‑150 (MQ=255)
                                                                                                                             ctgctgACACTGTTATGGCAGGGATTTTA‑TACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTG‑TTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTc                         <  1:2757853/150‑1 (MQ=255)
                                                                                                                               gctgACACTGTTATGGCAGGGGTTTTT‑TACTTTTTACGATTTTACCAAGTCGGGAAAGCCGGAACTGACGCCGGCGAATATCCGTGCGCTG‑TTTAATCCGGCGAATTACGACATCATTCTCCCCACGCTGACTATGGCTGTgggcgggaa                       >  2:3025791/1‑143 (MQ=255)
                                                                                                                                ctgACACTGTTATGGCAGGGATTTTA‑TACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTG‑TTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACt                      >  1:2606172/1‑150 (MQ=255)
                                                                                                                                  gACACTGTTATGGCAGGGGTTTTA‑TACTTTTTACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTG‑TTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTAt                    >  2:3709487/1‑150 (MQ=255)
                                                                                                                                   acacTGTTATGGCAGGGGTTTTT‑TACTTTTTACGATTTCACCATGTCGGTAAAGCCGGAAATGACGCTGGCGAATATCCGTGCGCTG‑TTTTATCCGGCGAATTACGACATCATTTTCCCCACGCTGACTATGGCTGTGGGAGTCACCATc                   >  2:2008227/1‑150 (MQ=255)
                                                                                                                                      cTGTTATGGCAGGGATTTTA‑TACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTG‑TTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGcc                <  2:882227/150‑1 (MQ=255)
                                                                                                                                        gTTATGGCAGGGATTTTA‑TACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTG‑TTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCAg              <  1:1290632/150‑1 (MQ=255)
                                                                                                                                             ggCAGGGATTTTA‑TACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTG‑TTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCAGCGCCa         >  2:3736513/1‑150 (MQ=255)
                                                                                                                                                 gggATTTTA‑TACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTG‑TTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCAGCGCCATTCt     >  1:1679210/1‑150 (MQ=255)
                                                                                                                                                 gggATTTTA‑TACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTG‑TTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCAGCGCCATTCt     >  2:3296355/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                       
TGCAATCACCTTCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTA‑TACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTG‑TTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCAGCGCCATTCTGGC  >  AP012306/1226409‑1226706

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑