Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123061,421,1470TG29.1% 453.6 / 15.0 235T79P (ACC→CCC) ydhBpredicted DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (93/73);  new base G (68/0);  total (162/73)
Fisher's exact test for biased strand distribution p-value = 3.11e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 295 total aligned reads displayed.

TTCCTGAAAGACAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCGGCACCGCCAGCCACTCTTCCAGCTGACGCACGGTATAGCTGACCGCAGAAGGAA  >  AP012306/1420998‑1421296
                                                                                                                                                     |                                                                                                                                                     
  ccTGAAAGACAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGa                                                                                                                                                     >  2:2746029/1‑150 (MQ=255)
    ttaaaGACAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATc                                                                                                                                                   >  2:712752/3‑150 (MQ=255)
    tGAAAGACAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATc                                                                                                                                                   >  1:4013090/1‑150 (MQ=255)
      aaaGACAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTg                                                                                                                                                 >  1:3694426/1‑150 (MQ=255)
      aaaGACAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTg                                                                                                                                                 >  1:878914/1‑150 (MQ=255)
      aaaGACAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGGTCTg                                                                                                                                                 >  2:3156920/1‑150 (MQ=255)
       aaGACAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGGTCTGc                                                                                                                                                >  1:4196789/1‑150 (MQ=255)
       aaGACAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCCGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGc                                                                                                                                                <  2:3604422/150‑1 (MQ=255)
         gACAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGcct                                                                                                                                              >  2:3287157/1‑148 (MQ=255)
           cAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGGTCTGCCttt                                                                                                                                            >  2:1334473/1‑150 (MQ=255)
           cAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCAttt                                                                                                                                            <  1:2795078/150‑1 (MQ=255)
           cAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCAttt                                                                                                                                            <  2:103734/150‑1 (MQ=255)
            aagaagTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGCTTTGCCtttt                                                                                                                                           >  2:2050797/1‑150 (MQ=255)
            aagaagTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCAtttt                                                                                                                                           >  1:173590/1‑150 (MQ=255)
             agaagTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCCttttt                                                                                                                                          >  1:4269411/1‑150 (MQ=255)
             agaagTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCAttttt                                                                                                                                          <  1:3882853/150‑1 (MQ=255)
              gaagTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCAtttttt                                                                                                                                         <  1:1244373/150‑1 (MQ=255)
              gaagTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCAtttttt                                                                                                                                         >  2:3727180/1‑150 (MQ=255)
               aagTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCAttttttt                                                                                                                                        <  2:311780/150‑1 (MQ=255)
               aagTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCGTCAttttttt                                                                                                                                        >  1:4170405/1‑150 (MQ=255)
                agTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCCtttttttt                                                                                                                                       >  1:2155083/1‑149 (MQ=255)
                agTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTg                                                                                                                                       <  1:1177270/150‑1 (MQ=255)
                 gTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTTCAtttttttta                                                                                                                                      >  1:1027021/1‑148 (MQ=255)
                       catcatCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACag                                                                                                                                >  1:3771341/1‑150 (MQ=255)
                         tcatcaAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCATTTTTTTGACAACagaa                                                                                                                              >  1:791857/1‑149 (MQ=255)
                         tcatcaAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGAGAACTTTATTTTTTTTACAACaaag                                                                                                                              >  2:117829/1‑150 (MQ=255)
                          catcaAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCA‑TTTTTTGACAACAGAgcg                                                                                                                            >  1:1434448/1‑150 (MQ=255)
                          catcaAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGGTCTGCATTTTTTTTACAACagagg                                                                                                                             >  2:2568879/1‑149 (MQ=255)
                            tcaAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCCTTTTTTTTACAACAGAgcgc                                                                                                                           >  2:2697095/1‑150 (MQ=255)
                            tcaAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTTACAACAGAgcgc                                                                                                                           >  2:827049/1‑150 (MQ=255)
                             caAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCATTTTTTTGACAACAGAGCGcc                                                                                                                          >  1:1697470/1‑150 (MQ=255)
                              aaaaTGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGccc                                                                                                                         <  1:3223211/150‑1 (MQ=255)
                              aaaaTGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGccc                                                                                                                         >  1:3978306/1‑150 (MQ=255)
                              aaaaTGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGGTTTTCATTTTTTTTTCAAAAAAGGGccc                                                                                                                         >  2:217943/1‑150 (MQ=255)
                              aaaaTGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGAGGTCTTCATTTTTTTTACAAAAAGGCGccc                                                                                                                         >  2:1858780/1‑150 (MQ=255)
                               aaaTGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGGTTTGCCTTTTTTTTTCAACAGGGCGCCct                                                                                                                        >  2:809014/1‑150 (MQ=255)
                               aaaTGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCATTTTTTTGACAACAAAGCGCCct                                                                                                                        >  1:1504897/1‑150 (MQ=255)
                                aaTGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCctt                                                                                                                       <  2:949847/150‑1 (MQ=255)
                                  tGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACCGAGCGCCcttct                                                                                                                     >  1:634914/1‑150 (MQ=255)
                                   ggCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAGCTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCcttctt                                                                                                                    <  1:732556/150‑1 (MQ=255)
                                    gCGATAAAAATCAACGATCATCTGCCGTATACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCttt                                                                                                                   <  2:1667058/150‑1 (MQ=255)
                                       aTAAAACTCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGGTCTTCGTTTTTTTCACAACAGAGCGCCCTTCTTTggg                                                                                                                >  2:1687379/1‑148 (MQ=255)
                                       aTAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTgag                                                                                                                <  1:2952849/150‑1 (MQ=255)
                                       aTAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCATTTTTTTTACAACACAGCGCCCTTCttttgg                                                                                                                >  2:1358773/1‑147 (MQ=255)
                                         aaaaaTCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGaa                                                                                                              <  2:2877503/150‑1 (MQ=255)
                                            aaTCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAAAAGAGCGCCCCTCCTTTAGaaaac                                                                                                           >  1:3161786/1‑148 (MQ=255)
                                             aTCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAAAGCGCCCCTCTTTGAGAAACCa                                                                                                          >  1:1352504/1‑150 (MQ=255)
                                              tCAACGATCATCTGCCGCGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGTGGATCTGGCGTTTTTTGACAACAGATCGCCCTCCTTTGTGAAACCAc                                                                                                         >  2:2386126/1‑150 (MQ=255)
                                               cAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACg                                                                                                        >  1:3562215/1‑150 (MQ=255)
                                               cAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGGTCTGCATTTTTTTTACAACAAAGCGCCCTTCTTTGAGAAACCACg                                                                                                        >  2:1509252/1‑150 (MQ=255)
                                                aaCGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCATTTTTTTTACAACAGAGCGCCCTTCTTTGAGAAACCACGc                                                                                                       >  1:1927623/1‑150 (MQ=255)
                                                 aCGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTTACAACAGAGCGCCCTTCTTTTAGAAACCACGCt                                                                                                      >  1:3136454/1‑150 (MQ=255)
                                                 aCGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCt                                                                                                      <  1:3426121/150‑1 (MQ=255)
                                                 aCGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCt                                                                                                      >  1:3357205/1‑150 (MQ=255)
                                                   gATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCCTTTTTTTGCCAAACGAGCGCCCTTCTTTGAGACACCCCGcccc                                                                                                    >  2:791923/1‑150 (MQ=255)
                                                   gATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTcc                                                                                                    >  1:2107719/1‑150 (MQ=255)
                                                    atcatcTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCca                                                                                                   <  1:1396995/150‑1 (MQ=255)
                                                     tcatcTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCcag                                                                                                  <  1:1823697/150‑1 (MQ=255)
                                                       atcTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTCTGCTGCCTTTTTTTTACAAAAGAACGGCCTTCTTTTGAAAACCACGCTccacca                                                                                                >  2:3963788/1‑150 (MQ=255)
                                                       atcTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCcagca                                                                                                >  1:1614785/1‑150 (MQ=255)
                                                        tcTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCcagcag                                                                                               >  1:374822/1‑150 (MQ=255)
                                                          tGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCg                                                                                             <  1:2748687/150‑1 (MQ=255)
                                                           gCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGCTCTGCATTTTTTTTGCAACAGAGCCCCCTTCTTTTCGAAACCACCCCCcaacaggcg                                                                                            >  2:3866924/1‑147 (MQ=255)
                                                           gCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCgg                                                                                            <  2:1800795/150‑1 (MQ=255)
                                                                 tACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGGTCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTTAGAAACCACCCTCCAGCAGCGGTCAGCt                                                                                      >  2:2677342/1‑150 (MQ=255)
                                                                    gTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGGTCTGCATTTTTTTTGCAACAAAGCGCCCTTCTTTGGGAAAACACGCTCCACCACCGGTCAGCCCca                                                                                   >  2:2493824/1‑150 (MQ=255)
                                                                    gTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCATTTTTTTGACAACCGAGCGCCCTTCTTTGAGAAACCCCGCTCCAGCAGCGGGCAGCTCca                                                                                   >  1:2116731/1‑150 (MQ=255)
                                                                      tCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTTACAACAGAGCGCCCTTCTTTTAGAAACCACGCTCCAGCAGCGGTCAGCTCcaca                                                                                 >  2:3048350/1‑150 (MQ=255)
                                                                       cTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCATTTTTTTTACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGGCAGCTCCCCAt                                                                                >  1:3299391/1‑150 (MQ=255)
                                                                               aCAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCATTTTTTTTACAACAGAGCGCCCTTCTTTTAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACCGTGc                                                                        >  1:1632520/1‑150 (MQ=255)
                                                                                cAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGCTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTTACAACAGAGCGCCCCTCTTTTAGAAACCACGCTCCCGCAGCGGTCAGCTCCACATCACGGTGcc                                                                       >  1:2002724/1‑150 (MQ=255)
                                                                                 aaTATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGGTCTGCATTTTTTTTACAACAGAGCGCCCTTCTTTTAGAAACCACGCCCCAGCAGCGGTCAGCTCCCCATCACCGTGCcg                                                                      >  2:3779286/1‑150 (MQ=255)
                                                                                    attatCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTTACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCcgtcg                                                                   >  2:1214183/1‑150 (MQ=255)
                                                                                         ccACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGGTCTGCATTTTTTTGACAACAGAGCGCCCCTCTTTGAGAAACCACGCTCCAGCAGCGGGCAGCTCCACATCACGGGGCCGTCGTTCaa                                                              >  2:1545022/1‑150 (MQ=255)
                                                                                          cACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCCCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGGTCcaa                                                             >  2:3251433/1‑150 (MQ=255)
                                                                                           aCTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAag                                                            <  2:747470/150‑1 (MQ=255)
                                                                                               cGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGGTCTGCATTTTTTTTACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCg                                                        >  1:4077570/1‑150 (MQ=255)
                                                                                                 aTAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCATTTTTTTTACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCCAAGAGCGGc                                                      >  1:2656749/1‑150 (MQ=255)
                                                                                                  tAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGGGGTGCATTTTTTTGACAAAAGAGGGGCCTTGTTTGAGAAAAAACGGGCCAGGAGGGGGGAGGTCCACAAGACGGGGGCGTCGTTCAAAGAGGGGCa                                                     >  2:547627/1‑150 (MQ=255)
                                                                                                  tAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCGGCa                                                     >  1:1485026/1‑150 (MQ=255)
                                                                                                   aGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCCAAGAGCGGCAc                                                    >  1:2433076/1‑150 (MQ=255)
                                                                                                     cTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCGGCACCg                                                  >  1:2327132/1‑150 (MQ=255)
                                                                                                       aaCTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCATTTTTTTGACAACAAAGCGCCCTTCTTTTAGAAACCACGCTCCAACAGCGGCCAGCTCCACATCACGGGGCCGTCGTTCAAAGAGCGGCACcccc                                                >  2:1443225/1‑150 (MQ=255)
                                                                                                          tGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGGTCTGCATTTTTTTTACAACAGAGCGCCCTTTTTTTAGAAACCACGCCCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGCTCAAAGAGCGGCACCgccagc                                             >  2:915567/1‑150 (MQ=255)
                                                                                                            aCCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACACTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCGGCACCgccagcca                                           >  1:3478810/1‑150 (MQ=255)
                                                                                                             ccGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGGTCTGCATTTTTTTTACAACAGAGCGCCCTTCTTTTAGAAACCACGCTCCAGCAGCGGGCAGCTCCACATCACGGGGCCGTCGTTCAAAGAGCGGCACCGCCAGCCAc                                          >  2:338500/1‑150 (MQ=255)
                                                                                                                   cAGCCGTTCGCTATCTGCTGACATTGCTTGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCCTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCGGCACCGCCAGCCACTCTTcc                                    >  1:3056178/1‑150 (MQ=255)
                                                                                                                             cTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTTACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCGGCACCGCCAGCCACTCTTCCAGCTGACGCa                          >  1:2252763/1‑150 (MQ=255)
                                                                                                                             cTATCTGCTGACATTGCTGGCGGGGGATCTGCATTTTTTTTAGAAAAAAGCGCCCTTCTTTGAGAAACCACGCTCCCGCAGCGGGCAGCTCCACATCACGGTGGCGTCGGTCAAAAAGCGGCACCGCCAGCCACTCCTCCAGCTGACGCa                          >  2:2419209/1‑150 (MQ=255)
                                                                                                                              tATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTTACAACAAAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCCGTCCGCTCCACATCACGGGGCCGTCGTTCAAAGAGGGGCACCGCCCGCCACTCTTCCAGCTGACGccc                         >  2:2933443/1‑148 (MQ=255)
                                                                                                                                   gctgACATTGCTGGCGGGGGATCTGCATTTTTTTTACAACAGAGCGCCCTTCTTTGAGAAACCACGCCCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCGGCCCCGCCAGCCACTCTTCCCGCTGACGCCCGGgat                    >  2:411560/1‑150 (MQ=255)
                                                                                                                                    ctgACATTGCTGGCGGGGGATCTGCTTTTTTTTGACAACAGAGCGCCCTTTTTTGTGAAACCACCCTCCCGCAGCGGTCAGCTCCACATCCCGGTGCCGTCGTTTAAAGAGCGGCGCCCCCACCCACTCTTTCAGCTGACGCCCGGtata                   >  2:4280/1‑150 (MQ=255)
                                                                                                                                     tgACATTGCTGGCGGGTGCTCTGCATTTTTTTTACAACAAAGGGCCCTTCTTTTAGAAAACACGCTCCAGCAGCGGTCAGCTCCACATCACGGGGCCGGCGTTCAAAAAGCGGCACCGCCAGCCCCTCTTCCCCCTGACGCACGGGATAg                  >  2:3368538/1‑150 (MQ=255)
                                                                                                                                     tgACATTGCTGGCGGGGGGGCTGCATTTTTTTGACAAAAGAGCGCCCCTCTTTGGGAAACCACGCTCCAGCAGCGGGCCGCTCCACATCACCGGGCCCGCGTTCAAAAAGCGGCCCCGCCCGCCCCCCTTTCAGCTGGCGCCCGGTGTAg                  >  2:1946857/1‑150 (MQ=255)
                                                                                                                                          ttGCTGGCGGGGGATCTGCATTTTTTTTACAACAGAGCGCCCTTCTTTGTGAAACCACCCTCCAGCAGCGGTCAGCTCCACATCACGGGGCCGTCGTTCAAAGAGCGGCCCCGCCAGCCACTCTTCCAGCTGACGCCCGGGATAGCTGAc             >  2:3840677/1‑150 (MQ=255)
                                                                                                                                                gCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCGGCACCGCCAGCCACTCTTCCAGCTGACGCACGGTATAGCTGACCGCAGa       >  1:2385992/1‑150 (MQ=255)
                                                                                                                                                  gggTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGGGAAACCACGCTCCAACAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCGGCACCGCCAGCCACTCTTCCAGCTGACGCCCGGTATAGCTGACCGCAGAAg     >  2:415145/1‑150 (MQ=255)
                                                                                                                                                  gggTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCGGCACCGCCAGCCACTCTTCCAGCTGACGCACGGTATAGCTGACCGCAGAAg     >  2:2255434/1‑150 (MQ=255)
                                                                                                                                                   ggTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCGGCACCGCCAGCCACTCTTCCAGCTGACGCACGGTATAGCTGACCGCAGAAgg    >  2:4013322/1‑150 (MQ=255)
                                                                                                                                                     tGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCGGCACCGCCAGCCACTCTTCCAGCTGACGCACGGTATAGCTGACCGCAGAAGGaa  >  1:1088313/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTCCTGAAAGACAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCGGCACCGCCAGCCACTCTTCCAGCTGACGCACGGTATAGCTGACCGCAGAAGGAA  >  AP012306/1420998‑1421296

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑