Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123062,212,7530AC23.9% 428.1 / 63.7 176Y241D (TAT→GAT) hcaTpredicted 3‑phenylpropionic transporter
Reads supporting (aligned to +/- strand):  ref base A (125/9);  new base C (0/42);  total (125/51)
Fisher's exact test for biased strand distribution p-value = 4.30e-32
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 227 total aligned reads displayed.

ACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAGCGCCAACCACGCAGACCAACCGGTG  >  AP012306/2212606‑2212901
                                                                                                                                                   |                                                                                                                                                    
aCGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGCCTTCCGCCACCACGCCCAGCGACCACAAATAcc                                                                                                                                                    <  1:126592/150‑1 (MQ=255)
 cGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATAccc                                                                                                                                                   >  1:4143629/1‑150 (MQ=255)
   ccGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACcaca                                                                                                                                                 <  2:2346017/150‑1 (MQ=255)
    cGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCAc                                                                                                                                                <  2:383278/150‑1 (MQ=255)
      tAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCAccg                                                                                                                                              <  2:1690657/150‑1 (MQ=255)
      tAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCAccg                                                                                                                                              <  2:3151376/150‑1 (MQ=255)
             tCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCgccgaggc                                                                                                                                       >  1:1725944/1‑150 (MQ=255)
                cATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACACCCAGCGACCACAAATACCCCACCgccgaggccga                                                                                                                                    >  1:3385716/1‑150 (MQ=255)
                aaTAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCCCCCCGCCCAGCGACCACAAATCCCCCACCgccgaggccga                                                                                                                                    <  2:1880207/149‑1 (MQ=255)
                  tAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTCCCGCCCCCACGCCCAGCGACCAAAAATCCCCCACCGCCGAGGCCGAGt                                                                                                                                  <  2:2876394/150‑1 (MQ=255)
                          cAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATCCCCCACCGCCGAGGCCGAGTAGCCAGct                                                                                                                          <  1:209732/150‑1 (MQ=255)
                          cAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGct                                                                                                                          >  1:1067009/1‑150 (MQ=255)
                           aGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCATCGCAAAGATAATGACTCCCGCCCCCCCGCCCAGCGCCCACAAATCCCCCACCGCCGAGGCCGAGTAGCCAGctg                                                                                                                         <  2:1531579/150‑1 (MQ=255)
                           aGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGctg                                                                                                                         >  1:3104422/1‑150 (MQ=255)
                             cccACTCCGCCGAAACCCGCCGAGATCAACAGCATACCGCGTGCACTACACGGGCGAAAAATTTTATTCCTCAGCGAAAAGATAATGACTCCCGCCCCCCCCCCCAGCGACCAAAAATCCCCCACCGCCGAGGCCGAGTAGCCAGctgcc                                                                                                                       <  2:2067056/148‑1 (MQ=255)
                              gcACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCCCGCCCAGCGCCCAAAAATCCCCCACCGCCGAGGCCGAGTAGCCAGctgcct                                                                                                                      <  2:3943949/150‑1 (MQ=255)
                               cACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGctgcctg                                                                                                                     >  2:251751/1‑150 (MQ=255)
                                 cTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTCCTCAGCGCAAAGATAATGACTTCCGCCCCCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCtgcctgcc                                                                                                                   <  1:3559562/150‑1 (MQ=255)
                                   aCGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGGTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAg                                                                                                                 >  1:3050732/1‑150 (MQ=255)
                                     gccgcAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCCCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTa                                                                                                               >  1:1022490/1‑150 (MQ=255)
                                     gccgaAAACCGCGGAAACAAACAGCATACCGCGTCCACAACAACGGGGAAAAATTTTTTTCCCCAGGCAAAAGAAAAGGTTTCCCGCCACCCCCCCAAGCCCCCAAAAATCCCCCCCCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTa                                                                                                               <  2:3091333/150‑1 (MQ=255)
                                        gcAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAAt                                                                                                            >  1:387290/1‑150 (MQ=255)
                                        gaaaaTCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGAAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCAAAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAAt                                                                                                            <  1:1405319/148‑1 (MQ=255)
                                           aaTCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGgc                                                                                                         <  1:4064311/150‑1 (MQ=255)
                                            aTCGCCGAGATCAACAGCATATCTCGTGCACTACAACGGCGGGAAACTTTATTACTCCGCCCAAAGCTAATGACTTCCCCCACCCCGCCCGCACACCCCAAATACCCCACCGCCCCAGCCCAATAGCCACCCGCCGGCCAGTcaagcccg                                                                                                        >  2:193054/1‑145 (MQ=255)
                                               gCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTa                                                                                                     >  1:3452114/1‑150 (MQ=255)
                                               gCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGAAAAAGTTTATTACTCAGCGAAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTa                                                                                                     <  1:3958185/150‑1 (MQ=255)
                                               gCCGAAACCAACAGCATACCGGGTGCACTACAACGGCGAAAAATTTTATTCCCCGGCAAAAAAATAATGCCTTCCGCCCCCCCCCCCAGCCACCAAAAATCCCCCCCCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTa                                                                                                     <  2:4068623/150‑1 (MQ=255)
                                                ccGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTaa                                                                                                    >  1:1940468/1‑150 (MQ=255)
                                                 cGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTaaa                                                                                                   >  2:790638/1‑150 (MQ=255)
                                                 cGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTaaa                                                                                                   >  1:3441668/1‑150 (MQ=255)
                                                  gagaTCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTaaaa                                                                                                  >  1:624263/1‑150 (MQ=255)
                                                  gagaTCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAAAGGCGCTaaaa                                                                                                  >  2:2057878/1‑150 (MQ=255)
                                                    gaTCAGCAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTTCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAAcc                                                                                                >  1:938298/1‑150 (MQ=255)
                                                    gaTCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAAcc                                                                                                >  1:3790312/1‑150 (MQ=255)
                                                       cAACAGCATATCGCGTGCACTACACCGGCGAAAAATTTTATTACTCAGCGAAAAGATAATGACTCCCGCCCCCACGCCCAGCGACCACAAATCCCCCCCCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTa                                                                                             <  2:2113250/150‑1 (MQ=255)
                                                       cAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTa                                                                                             >  2:3338129/1‑150 (MQ=255)
                                                       cAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTa                                                                                             >  2:1271721/1‑150 (MQ=255)
                                                       cAACAGCATACCGCGTGCACTACAACGGGGAAAAAGTTTATTACCCAGCGAAAAGAAAATGCCTCCCGCCACCCCGCCCAGCGCCCAAAAATCCCCCCCCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTa                                                                                             <  2:4122238/150‑1 (MQ=255)
                                                        aaCAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTaa                                                                                            >  1:2396539/1‑150 (MQ=255)
                                                        aaCAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTaa                                                                                            >  1:999834/1‑150 (MQ=255)
                                                        aaCAGCATATCGCGTGCACTACAACGGCGAAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCCCCACGCCCAGCGACCACAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTaa                                                                                            <  2:373032/150‑1 (MQ=255)
                                                           aGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAg                                                                                         >  2:363655/1‑150 (MQ=255)
                                                             catacggcgTGCACAACACGGGGGAAAAAGTTTATTACCCAGCCAAAAGAAAATGCCTCCCGCCCCCCCCCCCAGCGACCAAAAATCCCCCCCCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGc                                                                                       <  2:1246465/144‑1 (MQ=255)
                                                             cATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGc                                                                                       >  1:411845/1‑150 (MQ=255)
                                                             cATACCGCGTGCACTACAACGGCGAAAAAGTTTATTACTCAGCGAAAAGATAATGACTCCCGCCACCACGCCCAGCGCCCAAAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGc                                                                                       <  2:473359/150‑1 (MQ=255)
                                                              atacCGCGTGCACTAAACCGGCGAAAAAGTTTATTCCTCAGCGCAAAGATAATGCCTCCCGCCACCCCGCCCAGCGACCACAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGcc                                                                                      <  2:2324611/150‑1 (MQ=255)
                                                                atCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGc                                                                                    >  1:1343950/1‑150 (MQ=255)
                                                                accgcgTGCACTACAACGGCGAAAAAGTTTATTACTCAGCGCAAAGATAAGGCCTCCCCCCCCCACCCCCAGCGACCAAAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGc                                                                                    <  2:3919502/148‑1 (MQ=255)
                                                                  cgcgTGCACTACACCGGCGAAAAAGTTTATTACTCAGCGCAAAGATAATGCCTCCCGCCCCCCCGCCCAGCGCCCAAAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCAt                                                                                  <  2:4158723/150‑1 (MQ=255)
                                                                  cgcgTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCAt                                                                                  >  1:1320043/1‑150 (MQ=255)
                                                                  cgcgTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCAt                                                                                  >  1:4275190/1‑150 (MQ=255)
                                                                     gTGCACTACAACGGCGGAAAAGTTTATTACTCAGGGCAAAGATAATGGCTTCCCCCACCACGCCCAACGACCACAAATACCCCACCGCCGAGGCCGAGCAGCCAGCTGCCTGCCAGTAAATGACGCCAAAACCGCAATAGGCCGCCtgtg                                                                               >  2:3870019/1‑150 (MQ=255)
                                                                     gTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCAtgtg                                                                               >  1:674125/1‑150 (MQ=255)
                                                                     gTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCAtgta                                                                               >  1:91012/1‑149 (MQ=255)
                                                                     gTGCACAACAACGGGGAAAAATTTTATTACTCAGCGAAAAGATAAGGACTCCCGCCACCACGCCAAGCGACCACAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCAtgtg                                                                               <  2:104335/150‑1 (MQ=255)
                                                                        cACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGccc                                                                            >  1:839954/1‑150 (MQ=255)
                                                                         aCTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGcccc                                                                           >  2:1227952/1‑150 (MQ=255)
                                                                         aCTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGcccc                                                                           >  2:3974195/1‑150 (MQ=255)
                                                                          cTACACCGGCGAAAAAGTTTATTCCACAGCGAAAAGATAATGCCTCCCGCCACCACGCCCAGCGACCAAAAACCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGccccc                                                                          <  2:906852/150‑1 (MQ=255)
                                                                          cTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTCAATGGCGCTAAAACCGTAATAGGCCGCATGTGccccc                                                                          >  2:4045870/1‑150 (MQ=255)
                                                                           tACAACGGCGGAAAAGTTTATTCCTCAGCGAAAAGATAATGACTCCCGCCCCCCCGCCCGGCGCCCACAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCt                                                                         <  2:272875/150‑1 (MQ=255)
                                                                           tACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCt                                                                         >  2:262556/1‑150 (MQ=255)
                                                                              aaCGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCCCCACCACGCCCCGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCa                                                                      >  1:1173895/1‑150 (MQ=255)
                                                                                cggcggAAAAGTTTATTACTCAGCGCAAAGATAATGCCTCCCGCCCCCCCGCCCAGCGCCCACAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAAt                                                                    <  2:1064991/150‑1 (MQ=255)
                                                                                cggcggAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAAt                                                                    >  2:3701038/1‑150 (MQ=255)
                                                                                   ggaaaaaTTTTATTACCCAGCGAAAAAATAATGACTCCCGCCCCCACGCCGAGCGACCAAAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATaaa                                                                 <  2:1689491/147‑1 (MQ=255)
                                                                                    ggAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATaaac                                                                >  1:2964606/1‑150 (MQ=255)
                                                                                       aaaGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTTCAATaaacaaa                                                             >  1:2248322/1‑150 (MQ=255)
                                                                                       aaaGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATaaacaaa                                                             >  2:2616116/1‑150 (MQ=255)
                                                                                        aattttATTACCCAGCGCAAAGATAATGCCTCCCGCCACCACGCCCAGCGCCCACAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATaaacaaac                                                            <  2:765639/147‑1 (MQ=255)
                                                                                              attaCGCAGCGCAAAGATAATGACTCCCCCCCCCGCCCCTAGCGACCCAAAACCCCCCCCCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGc                                                      <  2:3753118/150‑1 (MQ=255)
                                                                                              ataccTCAGCGAAAAAAAAATGCCTCCCCCCCCCCCCCCCGGCAACAAAAAACCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGc                                                      <  2:4248052/146‑1 (MQ=255)
                                                                                                 aCTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAg                                                   >  2:599608/1‑150 (MQ=255)
                                                                                                 aCTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAg                                                   >  1:3319792/1‑150 (MQ=255)
                                                                                                    cAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGaaa                                                >  2:299107/1‑150 (MQ=255)
                                                                                                      gcgaAAAAATAATGACTCCCCCCCCCCCCCCCAGCGACCACAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAgc                                              <  2:681337/150‑1 (MQ=255)
                                                                                                        gcAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAgcgc                                            >  1:1840835/1‑150 (MQ=255)
                                                                                                        gcAAAGACAATCACTTCCGCCCCCACCCCCAGCGACCACAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAgcgc                                            <  2:2132049/150‑1 (MQ=255)
                                                                                                          aaaGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCa                                          >  2:2889314/1‑150 (MQ=255)
                                                                                                             gATAATGCCTCCCGCCCCCACGCCAAGCGACCACAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGtt                                       <  2:280626/150‑1 (MQ=255)
                                                                                                             gATAATGACTTCCGCCACCCCGCCCAGCGACCACAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGtt                                       <  1:1771548/150‑1 (MQ=255)
                                                                                                                aaTGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTg                                    >  1:720322/1‑150 (MQ=255)
                                                                                                                 tggACTCCCGCAACCACGCCCAGCGCCCACAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTgg                                   <  1:4172761/148‑1 (MQ=255)
                                                                                                                 aTGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTgg                                   >  1:402421/1‑150 (MQ=255)
                                                                                                                 aTGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTgg                                   >  1:3722543/1‑150 (MQ=255)
                                                                                                                 aTGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTgg                                   >  1:2899559/1‑150 (MQ=255)
                                                                                                                 aTGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTgg                                   >  1:2990956/1‑150 (MQ=255)
                                                                                                                  tGACTCCCGCCCCCCCCCCCGGCGACCAAAAACCCCCCCCCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGc                                  <  2:1853798/150‑1 (MQ=255)
                                                                                                                     cTTCCGCCCCCACGCCCAGCGACCACAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGaa                               <  1:3758875/150‑1 (MQ=255)
                                                                                                                      tcccGCCACCCCGCCCAGCGACCACAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAAc                              <  1:574307/148‑1 (MQ=255)
                                                                                                                         cGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAg                           >  2:1354280/1‑150 (MQ=255)
                                                                                                                          gCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAgc                          >  1:1595594/1‑150 (MQ=255)
                                                                                                                            caccacGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAgcgc                        >  1:3133632/1‑150 (MQ=255)
                                                                                                                             accacGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAGCGcc                       >  2:2003889/1‑150 (MQ=255)
                                                                                                                                       gCGACCAAAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAGCGCCAACCACGCAg             <  2:1901047/150‑1 (MQ=255)
                                                                                                                                         gACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAGCGCCAACCACGCAGAc           >  1:2663334/1‑150 (MQ=255)
                                                                                                                                         gACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAGCGCCAACCACGCAGAc           >  2:1195141/1‑150 (MQ=255)
                                                                                                                                             acaAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTACTGGCGAACCAGCGCCAACCACGCAGACCAAc       >  2:3430823/1‑150 (MQ=255)
                                                                                                                                              aaaaTCCCCCCCCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAGCGCCAACCACGCAGACCAAcc      <  2:269032/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
ACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAGCGCCAACCACGCAGACCAACCGGTG  >  AP012306/2212606‑2212901

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑