BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AP012306	3,614,960	G→T	R133L (CGC→CTC)	rpoC →	RNA polymerase, beta prime subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AP012306	3,614,960	0	G	T	100.0%	951.7 / NA	237	R133L (CGC→CTC)	rpoC	RNA polymerase, beta prime subunit
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (93/144); total (93/144)

Only 100 of 251 total aligned reads displayed.

TGTGAGAAGTGCGGCGTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGTTCGGTGACGAATTCGACGCGAAGATGGGGG  >  AP012306/3614815‑3615106

   gagaAGTGCGGCGTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCTCGATATc                                                                                                                                             >  1:3545260/1‑150 (MQ=255)

   gagaAGTGCGGCGTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCTCGATATc                                                                                                                                             >  1:1547887/1‑150 (MQ=255)

    agaAGTGCGGCGTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCTCGATATCg                                                                                                                                            <  1:3931542/150‑1 (MQ=255)

     gaAGTGCGGCGTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCTCGATATCGa                                                                                                                                           <  1:1787055/150‑1 (MQ=255)

      aaGTGCGGCGTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCTCGATATCGaa                                                                                                                                          >  2:3032308/1‑150 (MQ=255)

             gcgTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCTCGATATCGAACGCgtac                                                                                                                                   <  2:3275431/150‑1 (MQ=255)

               gTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCTCGATATCGAACGCgtactg                                                                                                                                 >  1:1072859/1‑150 (MQ=255)

                   aaGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCTCGATATCGAACGCgtactgtact                                                                                                                             <  2:3784394/150‑1 (MQ=255)

                   aaGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCTCGATATCGAACGCgtactgtact                                                                                                                             >  2:1000352/1‑150 (MQ=255)

                     gTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCTCGATATCGAACGCGTACTGTACttt                                                                                                                           <  1:3334984/150‑1 (MQ=255)

                       gccccAAACTAAAGTACGCCGTGAGCGTATGGGCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCTCGATATCGAACGCGTACTGTACTTTGa                                                                                                                         <  2:1185186/148‑1 (MQ=255)

                       gACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCTCGATATCGAACGCGTACTGTACTTTGa                                                                                                                         >  1:116325/1‑150 (MQ=255)

                             gACTAAAGTACGCCGTGAGCGTATGGGCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCTCGATATCGAACGCGTACTGTACTTTGAATCCTa                                                                                                                   >  2:2032019/1‑150 (MQ=255)

                             gACTAAAGTACGCCGTGAGCGTATGGGCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCTCGATATCGAACGCGTACTGTACTTTGAATCCTa                                                                                                                   >  1:2444944/1‑150 (MQ=255)

                              aCTAAAGTTCGCCGTGAGCGTATGGGCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCTCGATATCGAACGCGTACTGTACTTTGAATCCTAt                                                                                                                  <  2:3705174/150‑1 (MQ=255)

                              aCTAAAGTACGCCGTGAGCGTATGGGCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCTCGATATCGAACGCGTACTGTACTTTGAATCCTAt                                                                                                                  <  1:932186/150‑1 (MQ=255)

                               cTAAAGTACGCCGTGAGCGTATGGGCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCTCGATATCGAACGCGTACTGTACTTTGAATCCTAtg                                                                                                                 <  1:731472/150‑1 (MQ=255)

                               cTAAAGTACGCCGTGAGCGTATGGGCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCTCGATATCGAACGCGTACTGTACTTTGAATCCTAtg                                                                                                                 >  2:1301193/1‑150 (MQ=255)

                                tAAAGTACGCCGTGAGCGTATGGGCCTCATCGAACTGGCTTCCCCGACTGCGCACATGTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCTCGATATCGAACGCGTACTGTACTTTGAATCCTAtgt                                                                                                                <  2:3529239/150‑1 (MQ=255)

                                tAAAGTACGCCGTGAGCGTATGGGCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCTCGATATCGAACGCGTACTGTACTTTGAATCCTAtgt                                                                                                                >  1:2747822/1‑150 (MQ=255)

                                  aaGTACGCCGTGAGCGTATGGGCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCTCGATATCGAACGCGTACTGTACTTTGAATCCTATGTgg                                                                                                              >  2:1950090/1‑150 (MQ=255)

                                   aGTACGCCGTGAGCGTATGGGCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCTCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGt                                                                                                             <  1:3080721/150‑1 (MQ=255)

                                   aGTACGCCGTGAGCGTATGGGCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCTCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGt                                                                                                             <  1:3471555/150‑1 (MQ=255)

                                    gTACGCCGTGAGCGTATGGGCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCTCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGtt                                                                                                            >  1:378373/1‑150 (MQ=255)

                                    gTACGCCGTGAGCGTATGGGCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCTCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGtt                                                                                                            >  1:2488206/1‑150 (MQ=255)

                                    gTACGCCGTGAGCGTATGGGCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCTCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGtt                                                                                                            <  1:3954743/150‑1 (MQ=255)

                                       cGCCGTGTGATTATCGGCCACATCGAACTGGCCTCCCCGACTGCGCACATCTGGTTCCTGAAACCGCTCCCGTCCCGTATCGGTCTGCTGCTCGATATCCCGCTGCTCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATc                                                                                                         <  2:747646/150‑1 (MQ=255)

                                       cGCCGTGAGCGTATGGGCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCTCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATc                                                                                                         <  2:786870/150‑1 (MQ=255)

                                        gCCGTGAGCGTATGGGCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCTCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCg                                                                                                        <  1:1386028/150‑1 (MQ=255)

                                           gTGAGCGTATGGGCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCTCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAg                                                                                                     <  2:2247972/150‑1 (MQ=255)

                                           gTGAGCGTATGGGCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCTCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAg                                                                                                     <  2:3333141/150‑1 (MQ=255)

                                           gTGAGCGTATGGGCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCTCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAg                                                                                                     >  1:3102081/1‑150 (MQ=255)

                                                   aggggCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGGCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCTCGATATCGAACGCGTACTGTTCTTTGAATCCTATGTGGTTATCGAAGGCGGTATg                                                                                             <  2:4267403/148‑1 (MQ=255)

                                                   aTGGGCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCTCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATg                                                                                             >  1:2088570/1‑150 (MQ=255)

                                                     gggCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCTCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGAc                                                                                           <  2:653048/150‑1 (MQ=255)

                                                     gggCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCTCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGAc                                                                                           <  1:332246/150‑1 (MQ=255)

                                                       gCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCTCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCa                                                                                         <  2:4198963/150‑1 (MQ=255)

                                                       gCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCTCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCa                                                                                         <  1:1368229/150‑1 (MQ=255)

                                                        ccACATCGAACTGGCTCCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCTCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCaa                                                                                        <  1:4158527/150‑1 (MQ=255)

                                                          acaTCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCTCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAAcc                                                                                      <  1:3178161/150‑1 (MQ=255)

                                                          acaTCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCTCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAAcc                                                                                      >  2:67703/1‑150 (MQ=255)

                                                            aTCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCTCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTg                                                                                    <  1:295831/150‑1 (MQ=255)

                                                            aTCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCTCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTg                                                                                    <  1:1229668/150‑1 (MQ=255)

                                                             tCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCTCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTgg                                                                                   <  1:2538850/150‑1 (MQ=255)

                                                             tCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCTCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTgg                                                                                   <  2:184808/150‑1 (MQ=255)

                                                               gAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCTCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGaa                                                                                 >  2:4148705/1‑150 (MQ=255)

                                                                  cTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCTCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGt                                                                              <  2:1056400/150‑1 (MQ=255)

                                                                    ggCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCTCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTca                                                                            >  2:966473/1‑150 (MQ=255)

                                                                    ggCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCTCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTca                                                                            >  1:509845/1‑150 (MQ=255)

                                                                    ggCTTCCCCGACTGCGCACATCTAGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCTCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTca                                                                            <  1:1123145/150‑1 (MQ=255)

                                                                     gCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCTCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTcag                                                                           >  2:3937259/1‑150 (MQ=255)

                                                                     gCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCTCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTcag                                                                           <  1:1463931/150‑1 (MQ=255)

                                                                      cTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCTCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTcagc                                                                          >  1:2161630/1‑150 (MQ=255)

                                                                       ttCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCTCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTcagca                                                                         <  1:299298/150‑1 (MQ=255)

                                                                         ccccGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCTCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGa                                                                       <  2:179674/150‑1 (MQ=255)

                                                                           ccGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCTCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATc                                                                     <  2:2729744/150‑1 (MQ=255)

                                                                             gACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCTCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCct                                                                   >  2:2967723/1‑150 (MQ=255)

                                                                             gACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCTCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCct                                                                   <  1:1756270/150‑1 (MQ=255)

                                                                              aCTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCTCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCctg                                                                  <  2:247112/150‑1 (MQ=255)

                                                                                 gcgcACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCTCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCctgact                                                               <  2:2444944/150‑1 (MQ=255)

                                                                                  cgcACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCTCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCctgactg                                                              >  1:3683709/1‑150 (MQ=255)

                                                                                    cacaTCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCTCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGaa                                                            >  1:2668437/1‑150 (MQ=255)

                                                                                     acaTCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCTCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAag                                                           >  2:4089110/1‑150 (MQ=255)

                                                                                     acaTCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCTCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAag                                                           <  1:4126043/150‑1 (MQ=255)

                                                                                          tGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCTCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAg                                                      <  2:617766/150‑1 (MQ=255)

                                                                                            gTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCTCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTa                                                    <  2:1532280/150‑1 (MQ=255)

                                                                                               ccTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCTCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCt                                                 <  2:2194017/150‑1 (MQ=255)

                                                                                               ccTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCTCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCt                                                 <  2:3635940/150‑1 (MQ=255)

                                                                                                  gAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCTCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGa                                              <  2:938704/150‑1 (MQ=255)

                                                                                                     aTCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCTCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGAcgc                                           <  2:863475/150‑1 (MQ=255)

                                                                                                          tGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCTCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTgg                                      >  1:284579/1‑150 (MQ=255)

                                                                                                              gTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCTCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAaga                                  <  1:2782144/150‑1 (MQ=255)

                                                                                                               tCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCTCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAagag                                 <  1:2047628/150‑1 (MQ=255)

                                                                                                               tCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCTCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAagag                                 <  1:186179/150‑1 (MQ=255)

                                                                                                                cccGTATCGGTCTGCTGCTCGATATGCCGCTGCTCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGt                                >  1:2241939/1‑150 (MQ=255)

                                                                                                                cccGTATCGGTCTGCTGCTCGATATGCCGCTGCTCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGt                                <  1:1257635/150‑1 (MQ=255)

                                                                                                                 ccGTATCGGTCTGCTGCTCGATATGCCGCTGCTCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGtt                               >  1:187681/1‑150 (MQ=255)

                                                                                                                  cGTATCGGTCTGCTGCTCGATATGCCGCTGCTCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGTTc                              <  1:1077177/150‑1 (MQ=255)

                                                                                                                     aTCGGTCTGCTGCTCGATATGCCGCTGCTCGATATCGAACGTGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGTTCGGt                           <  2:276442/150‑1 (MQ=255)

                                                                                                                     aTCGGTCTGCTGCTCGATATGCCGCTGCTCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATGGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGTTCGGt                           <  1:3245708/150‑1 (MQ=255)

                                                                                                                       cGGTCTGCTGCTCGATATGCCGCTGCTCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGTACGCGCTGGAAGAGTTCGGTGa                         >  1:1833951/1‑150 (MQ=255)

                                                                                                                       cGGTCTGCTGCTCGATATGCCGCTGCTCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCATGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGTTCGGTGa                         <  2:3075563/150‑1 (MQ=255)

                                                                                                                         gTCTGCTGCTCGATATGCCGCTGCTCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGTTCGGTGACg                       <  2:486518/150‑1 (MQ=255)

                                                                                                                          tCTGCTGCTCGATATGCCGCTGCTCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGTTCGGTGACGa                      >  1:2711086/1‑150 (MQ=255)

                                                                                                                            tgctgcTCGATATGCCGCTGCTCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGTTCGGTGACGAAt                    <  2:3143065/150‑1 (MQ=255)

                                                                                                                            tgctgcTCGATATGCCGCTGCTCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGTTCGGTGACGAAt                    >  2:1399602/1‑150 (MQ=255)

                                                                                                                             gctgctCGATATGCCGCTGCTCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGTTCGGTGACGAAtt                   <  1:439031/150‑1 (MQ=255)

                                                                                                                             gctgctCGATATGCCGCTGCTCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGTTCGGTGACGAAtt                   >  1:1964808/1‑150 (MQ=255)

                                                                                                                                gctCGATATGCCGCTGCTCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGTTCGGTGACGAATTCGa                <  1:2835887/150‑1 (MQ=255)

                                                                                                                                 ctCGATATGCCGCTGCTCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGTTCGGTGACGAATTCGAc               <  1:183848/150‑1 (MQ=255)

                                                                                                                                   cGATATGCCGCTGCTCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTcag                                                                           <  1:693827/88‑1 (MQ=255)

                                                                                                                                   cGATATGCCGCTGCTCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTcag                                                                           >  2:693827/1‑88 (MQ=255)

                                                                                                                                   cGATATGCCGCTGCTCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGTTCGGTGACGAATTCGAcgc             >  2:1661486/1‑150 (MQ=255)

                                                                                                                                   cGATATGCCGCTGCTCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGTTCGGTGACGAATTCGAcgc             >  1:2371810/1‑150 (MQ=255)

                                                                                                                                      tatGCCGCTGCTCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGTTCGGTGACGAATTCGACGCGaa          <  2:2932087/150‑1 (MQ=255)

                                                                                                                                      tatGCCGCTGCTCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGTTCGGTGACGAATTCGACGCGaa          >  1:2370564/1‑150 (MQ=255)

                                                                                                                                        tGCCGCTGCTCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGTTCGGTGACGAATTCGACGCGAAGa        <  2:3155626/150‑1 (MQ=255)

                                                                                                                                          ccGCTGCTCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGTTCGGTGACGAATTCGACGCGAAGATg      >  2:145383/1‑150 (MQ=255)

                                                                                                                                            gctgctCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGTTCGGTGACGAATTCGACGCGAAGATggg    >  2:4237735/1‑150 (MQ=255)

                                                                                                                                             cTGCTCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTACCGTAGGCGGTATGCCCAACCTGGAACGTCAGCAGATCCCGACTGAGGAGCAGTATCTGGACACGCTGGAAAAGTTGGGGGCTGTATCCGCCGCGAAGAGgggg   >  2:4014775/1‑150 (MQ=255)

TGTGAGAAGTGCGGCGTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGTTCGGTGACGAATTCGACGCGAAGATGGGGG  >  AP012306/3614815‑3615106

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`