Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123061,226,5570AG23.2% 428.6 / 72.5 185G55G (GGA→GGG) ydcUpredicted spermidine/putrescine transporter subunit
Reads supporting (aligned to +/- strand):  ref base A (19/123);  new base G (43/0);  total (62/123)
Fisher's exact test for biased strand distribution p-value = 1.69e-27
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 210 total aligned reads displayed.

TGCAATCACCTTCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTC‑GGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGC‑GAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCAGCGCCATTCTGGC  >  AP012306/1226409‑1226706
                                                                                                                                                    |                                                                                                                                                       
tGCAATCACCTTCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGAt                                                                                                                                                        <  1:927683/150‑1 (MQ=255)
 gCAATCACCTTCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGAtt                                                                                                                                                       <  2:2221280/150‑1 (MQ=255)
 gCAATCACCTTCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGAtt                                                                                                                                                       <  2:476757/150‑1 (MQ=255)
 gCAATCACCTTCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGAtt                                                                                                                                                       <  1:847994/150‑1 (MQ=255)
 gCAATCACCTTCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGAtt                                                                                                                                                       <  2:3835196/150‑1 (MQ=255)
   aaTCACCTTCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGtttt                                                                                                                                                     >  2:3537406/1‑150 (MQ=255)
     tCACCTTCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTtat                                                                                                                                                   <  1:455616/150‑1 (MQ=255)
     tCACCTTCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTtat                                                                                                                                                   <  1:3120993/150‑1 (MQ=255)
      cACCTTCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTtata                                                                                                                                                  <  2:3661044/150‑1 (MQ=255)
       aCCTTCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATAc                                                                                                                                                 <  1:2731340/150‑1 (MQ=255)
        ccTTCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGtttttttct                                                                                                                                                >  2:1250022/1‑145 (MQ=255)
          ttCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACttt                                                                                                                                              >  1:252215/1‑150 (MQ=255)
          ttCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGAGTTTTTACttt                                                                                                                                              >  1:1370813/1‑150 (MQ=255)
              cGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGAc                                                                                                                                          <  1:2265519/150‑1 (MQ=255)
               gTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACg                                                                                                                                         <  1:1955401/150‑1 (MQ=255)
                 ccAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGAt                                                                                                                                       <  2:1909186/150‑1 (MQ=255)
                  cAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTTACGAtt                                                                                                                                      >  1:1759413/1‑150 (MQ=255)
                   aGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGAttt                                                                                                                                     <  1:3803572/150‑1 (MQ=255)
                    ggTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTc                                                                                                                                    <  2:1985064/150‑1 (MQ=255)
                    ggTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTc                                                                                                                                    <  2:1585929/150‑1 (MQ=255)
                        tGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGGTTTTTACTTTTTTCGAttttccca                                                                                                                                >  2:1850235/1‑145 (MQ=255)
                          ggTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATg                                                                                                                              <  1:2360611/150‑1 (MQ=255)
                          ggTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATg                                                                                                                              <  1:3135538/150‑1 (MQ=255)
                            tAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTc                                                                                                                            <  2:128229/150‑1 (MQ=255)
                             aaGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTC‑g                                                                                                                          <  1:2813032/150‑1 (MQ=255)
                              aGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTACTTTTTACGAATTTACCATGTC‑gg                                                                                                                         >  1:1000869/1‑150 (MQ=255)
                               ggTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTC‑GGt                                                                                                                        <  2:2265269/150‑1 (MQ=255)
                                   tCCGGTTTCTTCTGGCATAATCCGGGGGTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTC‑GGTAACg                                                                                                                    <  2:1144276/150‑1 (MQ=255)
                                      ggTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTAATTTTTATGAATTCACCCTTTC‑GGTAACTCCg                                                                                                                 >  2:4068076/1‑150 (MQ=255)
                                         ttcttcTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTATACTTTTTACGATTTTACCATGTC‑GGTAACGCCCGaa                                                                                                              >  1:1896666/1‑150 (MQ=255)
                                          tcttctGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTC‑GGTAACGCCGGAAc                                                                                                             <  1:1249812/150‑1 (MQ=255)
                                           cttctGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTC‑GGTAACGCCGGAACt                                                                                                            <  1:1276537/150‑1 (MQ=255)
                                             tctGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTACTTTTTACGATTTTACCATTTC‑GGTAACCCCGGAACtta                                                                                                          >  1:1099949/1‑148 (MQ=255)
                                              ctGGCATACTCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCCCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCCCCATGTC‑GGTAACGCCGGAACTgcc                                                                                                         <  2:2034328/150‑3 (MQ=255)
                                                ggCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTC‑GGTAACGCCGGAACTGACGc                                                                                                       <  2:1419459/150‑1 (MQ=255)
                                                ggCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTC‑GGTAACGCCGGAACTGACGc                                                                                                       <  1:2295465/150‑1 (MQ=255)
                                                  cATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTC‑GGTAACGCCGGAACTGACGCTg                                                                                                     <  1:3949303/150‑1 (MQ=255)
                                                   aTAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTTCTTTTTTCGCTTTTACCCTGTC‑GGGAAAGCCCGGACTGACGCTgg                                                                                                    >  2:285641/1‑150 (MQ=255)
                                                    tAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTTTACTTTTTACGGTTTCACCCTGTC‑GGGAACGCCGGGACTGACGCTGGc                                                                                                   >  1:504058/1‑150 (MQ=255)
                                                     aaTCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTTTACTTTTGTCGATTTTACCCTGGC‑GGTAACGCCGGGACTGATGCTGGCg                                                                                                  >  1:1399958/1‑150 (MQ=255)
                                                       tCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTC‑GGTAACGCCGGAACTGACGCTGGCGaa                                                                                                <  1:405937/150‑1 (MQ=255)
                                                         cGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTC‑GGTAACGCCGGAACTGACGCTGGCGAata                                                                                              <  1:3998155/150‑1 (MQ=255)
                                                          ggggctgtggctGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTC‑GGTAACGCCGGAACTGACGCTGGCGAatat                                                                                             <  2:2682250/150‑1 (MQ=255)
                                                          ggggctggggctGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTACTTTTTTCGATTTCACCCTGTC‑GGTAAAGCCGGAACTGACGCCGGCGAatat                                                                                             >  2:305949/1‑150 (MQ=255)
                                                            ggctggggctgTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTACTTTTTTCGGTTTTTCCATGTCGGGTAAGCCCGGAA‑TGACGCTGGCGGatatac                                                                                           >  2:4194171/1‑148 (MQ=255)
                                                             gctggggctgTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTC‑GGTAACGCCGGAACTGACGCTGGCGAATATCCg                                                                                          <  2:1175232/150‑1 (MQ=255)
                                                               tggggctgTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTC‑GGTAACGCCGGAACTGACGCTGGCGAATATCCGTg                                                                                        <  2:1684631/150‑1 (MQ=255)
                                                                 gggctgTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTTATTTTTACGATTTTCCCCTCTC‑GGTAACGGCGGGACGGGGGCTTGGGAATATCCgctcg                                                                                      >  2:2230192/1‑146 (MQ=255)
                                                                   gctgTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTC‑GGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCt                                                                                    <  2:2446302/150‑1 (MQ=255)
                                                                    ctgTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTTTACTTTTTACGATTTCACCATGTC‑GGTAACGCCGGGACTGACGCTGGCGGATATTCGTGCGCTg                                                                                   >  1:2944581/1‑150 (MQ=255)
                                                                     tgTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTACTTTTGACGATTTTACCATGTC‑GGTAACGCCCGAACTGACGCTGGCGGATATCCCTGCGCTGt                                                                                  >  2:380148/1‑150 (MQ=255)
                                                                      gTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTC‑GGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGtt                                                                                 <  1:1503718/150‑1 (MQ=255)
                                                                      gTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTC‑GGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGtt                                                                                 <  1:2493767/150‑1 (MQ=255)
                                                                        ttttACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGAGACTGTTATGGCAGGGGGGTTTTAATTTTTTCGGTTTCACCCTGTCGGGTAAGGCC‑GCACCGACGCTTGGGAATATACGGGGGCTGtttt                                                                               >  2:2916350/1‑149 (MQ=255)
                                                                           tACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTATACTTTTTTCGATTTTACCATGTC‑GGTAACGGCGGGAATTACGCTGGGGAAAATCCGTGCGCTGTTTTATc                                                                            >  2:3493119/1‑150 (MQ=255)
                                                                           tACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTC‑GGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATc                                                                            <  1:1217734/150‑1 (MQ=255)
                                                                           tACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTC‑GGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATc                                                                            <  2:790489/150‑1 (MQ=255)
                                                                           tACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTC‑GGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATc                                                                            <  1:168586/150‑1 (MQ=255)
                                                                              tgctgcTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTC‑GGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCgg                                                                         <  2:1537179/150‑1 (MQ=255)
                                                                              tgctgcTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTC‑GGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCgg                                                                         <  1:3519001/150‑1 (MQ=255)
                                                                                       gCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTC‑GGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGC‑GAATTACg                                                               <  2:1782928/150‑1 (MQ=255)
                                                                                           gCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTATACTTTTGACGATTTCACCATGTC‑GGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGC‑GAATTACGAcat                                                           >  1:3866645/1‑150 (MQ=255)
                                                                                            cTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTC‑GGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGC‑GAATTACGAcatc                                                          <  1:690191/150‑1 (MQ=255)
                                                                                            cTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTC‑GGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGC‑GAATTACGAcatc                                                          <  1:2644427/150‑1 (MQ=255)
                                                                                             tAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTC‑GGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGC‑GAATTACCAcatca                                                         <  1:3295309/150‑1 (MQ=255)
                                                                                              aaTGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTTCTTTTTACGATTTTCCCATGTC‑GGGAAAGCCCGGACTGACGCTGGCGGATATTCGTGCGCTGTTTTATTCCGC‑GGAATACGAcatcat                                                        >  2:2532509/1‑150 (MQ=255)
                                                                                              aaTGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTACTTTTTACGATTTTACCCTGTC‑GGGAAAGCCCGGACTGACGCTGGCGGATATCCGTGCGCTGTTTTATTCGGG‑GAATTACGAaatcat                                                        >  2:825040/1‑150 (MQ=255)
                                                                                              aaTGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTATAATTTTTACGATTTTACCATGTC‑GGTAACGCCGGGACTGACGCTGGCGAATATTCGTGCGCTGTTTTATTCGGC‑GAATTACGAcatcat                                                        >  2:2125425/1‑150 (MQ=255)
                                                                                               aTGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGGTTTTTTCTTTTTTCGGTTTTTCCCTGTC‑GGTAACGCCGGCACCGCGGCCGGCGGATATCTTTGCGCTGTTTTATCCGGG‑GAGTTTCGACATCAtt                                                       >  2:2754765/1‑150 (MQ=255)
                                                                                               aTGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTC‑GGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGC‑GAATTACGACATCAtt                                                       <  1:721667/150‑1 (MQ=255)
                                                                                               aTGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTC‑GGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGC‑GAATTACGACATCAtt                                                       <  1:1625257/150‑1 (MQ=255)
                                                                                                tgtgGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTC‑GGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGC‑GAATTACGACATCATtc                                                      <  1:1191743/150‑1 (MQ=255)
                                                                                                 gtgGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTC‑GGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGC‑GAATTACGACATCATtct                                                     <  2:3385236/150‑1 (MQ=255)
                                                                                                 gtgGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTCATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTC‑GGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGC‑GAATTACGACATCATtct                                                     <  1:992671/150‑1 (MQ=255)
                                                                                                    gTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGATTTTTAATTTTTACGACTTTCCCCTCTT‑GGGAACGCCGGAAGCGTGGCTGGGGGAAAATCGGGCGCTGTTTAATTCTGCGGAATTAC‑TCATCATTTTccc                                                  >  2:3247128/1‑149 (MQ=255)
                                                                                                     tttGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTACTTTTTACGATTTCACCATGTC‑GGTAACGCCGGGACTGACGCCGGCGAATATCCGTGCGCTGTTTTATCCGGC‑GGATTTCGACATCATTCTCCGc                                                 >  2:4145559/1‑150 (MQ=255)
                                                                                                             tGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTACTTTTTGCGATTTCCCCATGTC‑GGTAACGCCGGAACTGACGCTGGCGGATATCCGTGCGCTGTTTAATCCGGC‑GGATTACGACATCATTCTCCGCACGCCGAc                                         >  2:3439560/1‑150 (MQ=255)
                                                                                                             tGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTC‑GGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGC‑GAATTACGACATCATTCTCCGCACGCTGAc                                         <  1:2161087/150‑1 (MQ=255)
                                                                                                              gTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTC‑GGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGC‑GAATTACGACATCATTCTCCGCACGCTGACt                                        <  2:3730569/150‑1 (MQ=255)
                                                                                                               tCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTTTTTTTTTTGCGGTTTTACCATGTC‑CGTAACGCCGGGAATGACGCTGGGGGATAACCCTGCGCTGTTTTATTCGGC‑GAATTAAGACATCATTCTCCCCACGCCGGCTa                                       >  2:3167014/1‑150 (MQ=255)
                                                                                                               tCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTC‑GGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGC‑GAATTACGACATCATTCTCCGCACGCTGACTa                                       <  1:3754427/150‑1 (MQ=255)
                                                                                                                 tATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTAATTTTTACGATTTTACCATGTC‑GGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTTATCCGGC‑GAATTACGACATCATTCTCCGCACGCTGACTATg                                     >  2:572474/1‑150 (MQ=255)
                                                                                                                  aTTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTTTACTTTTTACGATTTCACCATGTC‑GGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGC‑GAATTACGACATCATTCTCCGCACGCTGACTATgg                                    >  1:458309/1‑150 (MQ=255)
                                                                                                                    ttCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTC‑GGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGC‑GAATTACGACATCATTCTCCGCACGCTGACTATGGCt                                  <  1:2617048/150‑1 (MQ=255)
                                                                                                                       ggCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTC‑GGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGC‑GAATTACGACATCATTCTCCGCACGCTGACTATGGCtgtg                               <  2:1927616/150‑1 (MQ=255)
                                                                                                                        gCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTAATTTTGACGACTTCACCATGTT‑GGTTACGCCCGGACTGACGCTGGCGGAAATCCGTGCGCCGTTTTATCCGGC‑GAATTACGACATCATTTTCCGCACCCTCGCTATTGCtcggg                              >  2:346441/1‑146 (MQ=255)
                                                                                                                                ctgACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTC‑GGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGC‑GAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACt                      <  2:3876317/150‑1 (MQ=255)
                                                                                                                                  gACACTGTTATGGCAGGGGTTTTTTACTTTTGACGATTTCACCATGTC‑GGTAAAGCCGGGAATGACGCTGGCGAATATCCGTGCGCTGTTTAATTCGGC‑GAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGGAGGCACCAt                    >  2:3002732/1‑150 (MQ=255)
                                                                                                                                  gACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTC‑GGTAACGCCGGATCTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGC‑GAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTAt                    <  2:445303/150‑1 (MQ=255)
                                                                                                                                   acacTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTC‑GGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGC‑GAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATc                   <  1:3373835/150‑1 (MQ=255)
                                                                                                                                      cTGTTATGGCAGGGGTTTTATACTTTTTACGATTTTACCATGTC‑GGGAAAGCCGGGAATGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGC‑GAATTACGACATCATTCTCCCCACGCTGACTATGGCTGTGGGAGGCACCATCGcc                >  2:4139473/1‑150 (MQ=255)
                                                                                                                                         ttATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTC‑GGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGC‑GAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCAgc             <  1:3671951/150‑1 (MQ=255)
                                                                                                                                         ttATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTC‑GGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGC‑GAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCAgc             <  1:3423977/150‑1 (MQ=255)
                                                                                                                                           aTGGCAGGGATTTTATACTTTTGACGATTTCACCATGTC‑GGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGC‑GAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCAgcgc           <  2:2965528/150‑1 (MQ=255)
                                                                                                                                             ggCAGGGATTTTATACTTTTGACGATTTCACCATGTC‑GGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGC‑GAATTACGACATCATTCTCCGCACGCTTACTATGGCTGTGGCAGTCACTATCGCCAgcgc           >  1:301245/1‑148 (MQ=255)
                                                                                                                                               cAGGGATTTTATACTTTTGACGATTTCACCATGTC‑GGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGC‑GAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCAGCGCCAtt       <  2:638881/150‑1 (MQ=255)
                                                                                                                                                  ggATTTTATACTTTTGACGATTTCACCATGTC‑GGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGC‑GAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCAGCGCCATTCTg    <  2:71481/150‑1 (MQ=255)
                                                                                                                                                  ggATTTTATACTTTTGACGATTTCACCATGTC‑GGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGC‑GAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCAGCGCCATTCTg    >  2:3347283/1‑150 (MQ=255)
                                                                                                                                                   gATTTTATACTTTTGACGATTTCACCATGTC‑GGTAACGCCGGAACTGACGCTGGCGATTATCCGTGCGCTGTTTAATCCGGC‑GAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCAGCGCCATTCTgg   <  1:1879527/150‑1 (MQ=255)
                                                                                                                                                   gATTTTATACTTTTGACGATTTCACCATGTC‑GGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGC‑GAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCAGCGCCATTCTgg   >  2:2351894/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                       
TGCAATCACCTTCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTC‑GGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGC‑GAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCAGCGCCATTCTGGC  >  AP012306/1226409‑1226706

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑