Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123061,854,8510AC29.0% 615.1 / 70.6 269T25P (ACC→CCC) yejHpredicted ATP‑dependet helicase
Reads supporting (aligned to +/- strand):  ref base A (167/24);  new base C (0/78);  total (167/102)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 306 total aligned reads displayed.

TAGCGGTCTAATAACATCCCTCAAGGGAAAAAGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGCAAAGTATCAGGCGCTGGGGCTG  >  AP012306/1854702‑1855000
                                                                                                                                                     |                                                                                                                                                     
taGCGGTCTAATAACATCCCTCAAGGGAAAAAGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATaaaa                                                                                                                                                       <  2:3164966/150‑1 (MQ=255)
     gTCTAATAACATCCCTCAAGGGAAAAAGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCATCGTCATAAAACCCCt                                                                                                                                                  >  2:197011/1‑150 (MQ=255)
        taataaCATCCCTCAAGGGAAAAAGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGcc                                                                                                                                               >  1:2632818/1‑150 (MQ=255)
         aataaCATCCCTCAAGGGAAAAAGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCg                                                                                                                                              >  1:1363904/1‑150 (MQ=255)
          ataaCATCCCTCAAGGGAAAAAGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGt                                                                                                                                             >  1:4054732/1‑150 (MQ=255)
           taaCACCCCTCAAGGAAAAAAGACCCATGGCATACTATTAGCAAAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAACCCCCTGCCGtt                                                                                                                                            <  2:3999120/150‑1 (MQ=255)
              cATCCCTCAAGGGAAAAAGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATc                                                                                                                                         >  2:1605433/1‑150 (MQ=255)
                    tCAAGGGAAAAAGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGtgctg                                                                                                                                   >  2:2695156/1‑150 (MQ=255)
                      aggggAAAAAGACCCAGGGCATACTATTAGAAGAATAATCTACCTACGCGAGCCCATGATTTTTCCACTCCCCCCATTCCAGAAAGAGGCCGTGGATGCCACGCCCAACCTTTTCCGCCGTAAAAAAACCCCTGCCGTTATCGTGCTGcc                                                                                                                                 <  2:1005727/148‑1 (MQ=255)
                      aaGGGAAAAAGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGcc                                                                                                                                 >  2:3682597/1‑150 (MQ=255)
                      aaGGGAAAAAGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGcc                                                                                                                                 >  2:1266294/1‑150 (MQ=255)
                       aGGGAAAAAGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGccc                                                                                                                                >  2:1902636/1‑150 (MQ=255)
                       aGGGAAAAAGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGccc                                                                                                                                >  2:1522466/1‑150 (MQ=255)
                        gggAAAAAGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCa                                                                                                                               >  1:3866830/1‑150 (MQ=255)
                          gAAAAAGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCAcc                                                                                                                             >  1:843461/1‑150 (MQ=255)
                          gAAAAAGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCAcc                                                                                                                             >  1:2430968/1‑150 (MQ=255)
                           aaaaaGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCg                                                                                                                            >  1:3200970/1‑150 (MQ=255)
                            aaaaGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCgg                                                                                                                           <  1:1121476/150‑1 (MQ=255)
                             aaaGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGgc                                                                                                                          <  1:754399/150‑1 (MQ=255)
                               aGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGgcgc                                                                                                                        >  2:2933658/1‑150 (MQ=255)
                                gATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCa                                                                                                                       >  1:327633/1‑150 (MQ=255)
                                 aTCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCACCCATTTTCGTCGTCAAAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAg                                                                                                                      <  2:1724349/150‑1 (MQ=255)
                                 aTCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAg                                                                                                                      <  1:476307/150‑1 (MQ=255)
                                   ccATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTAGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGt                                                                                                                    >  2:4113120/1‑150 (MQ=255)
                                     aTGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTaa                                                                                                                  <  1:3249931/150‑1 (MQ=255)
                                      tGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTaaa                                                                                                                 >  2:2586824/1‑150 (MQ=255)
                                      tGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTaaa                                                                                                                 >  1:3383279/1‑150 (MQ=255)
                                       ggCATCCTATTAGCAGAATAATCTCCCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCTTTTTCGTCGTCAAAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTaaaa                                                                                                                <  2:2430814/150‑1 (MQ=255)
                                       ggCATACTATTAGCAGAATAATCTCCCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTAAAAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTaaaa                                                                                                                <  2:3484649/150‑1 (MQ=255)
                                        gCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAg                                                                                                               >  2:210285/1‑150 (MQ=255)
                                         cATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGc                                                                                                              >  2:3426877/1‑150 (MQ=255)
                                           tACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCt                                                                                                            >  2:2674821/1‑150 (MQ=255)
                                           tACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCt                                                                                                            >  1:1776257/1‑150 (MQ=255)
                                              tATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGt                                                                                                         >  1:3816819/1‑150 (MQ=255)
                                                  aGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATa                                                                                                     >  1:2397722/1‑150 (MQ=255)
                                                  aGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATa                                                                                                     >  1:802597/1‑150 (MQ=255)
                                                   gCAGAATAATCTCCCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAg                                                                                                    <  1:2071444/150‑1 (MQ=255)
                                                   gCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAGACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAg                                                                                                    >  2:1042299/1‑150 (MQ=255)
                                                   gCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAg                                                                                                    <  1:1447205/150‑1 (MQ=255)
                                                   gCAGAAAAATCTCCCCACGAGAGCCCATTATTTTTACATTTCGCCCATATCAGCAAGAAGCCGTGGATCCCACGCTCAACCTTTTTCGTCGTAATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAg                                                                                                    <  2:2116583/150‑1 (MQ=255)
                                                    cAGAATAATCCTCCTACGGGGGACCATGATTTTTACCCTTCCCCCAAATCAGCAAGAAGCCGTGGATGCCCCGCTCCCCCATTTTCGTCGTCAAAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGc                                                                                                   <  2:1458551/150‑1 (MQ=255)
                                                     aGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCg                                                                                                  <  1:2241349/150‑1 (MQ=255)
                                                       aataatCTACCGCCGCGAGACCATGATTTTTACACTTCCCCCATATCAGAAAGAACCGGTGGATGCCACCCCCACCCATTTTCGCCGTAATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGa                                                                                                <  2:2345846/150‑1 (MQ=255)
                                                        ataatCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGaa                                                                                               >  2:3933238/1‑150 (MQ=255)
                                                        ataatCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGaa                                                                                               >  2:589012/1‑150 (MQ=255)
                                                          aatCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACt                                                                                             >  1:3481443/1‑150 (MQ=255)
                                                          aatCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACt                                                                                             >  1:1556287/1‑150 (MQ=255)
                                                           atCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTg                                                                                            >  2:1661330/1‑150 (MQ=255)
                                                                cctacGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACg                                                                                       >  2:524570/1‑150 (MQ=255)
                                                                      cgAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCCCggctggc                                                                                 >  2:3550453/1‑150 (MQ=255)
                                                                       gagaCCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACggctggct                                                                                <  1:2925827/150‑1 (MQ=255)
                                                                           ccATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTg                                                                            >  1:2793577/1‑150 (MQ=255)
                                                                            cATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTgg                                                                           <  2:3857267/150‑1 (MQ=255)
                                                                            cATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTgg                                                                           >  1:1950153/1‑150 (MQ=255)
                                                                            cATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTgg                                                                           >  2:2305958/1‑150 (MQ=255)
                                                                             attaTTTTTACCCTTCGCCCATACCAGCAAGAGGCCGTGGATGCCACGCTCACCCTTTTCCGTCGCCAAAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGt                                                                          <  2:4016730/147‑1 (MQ=255)
                                                                              tGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTc                                                                         <  1:3022487/150‑1 (MQ=255)
                                                                              tGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTc                                                                         >  1:2286129/1‑150 (MQ=255)
                                                                               gATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTcg                                                                        >  2:2505279/1‑150 (MQ=255)
                                                                               gATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTcg                                                                        >  1:1298030/1‑150 (MQ=255)
                                                                                   tttACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCgtg                                                                    >  1:3326400/1‑150 (MQ=255)
                                                                                      acacTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCgtgctg                                                                 >  1:3544931/1‑150 (MQ=255)
                                                                                        acTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCgtgctggt                                                               >  1:2021347/1‑150 (MQ=255)
                                                                                           tCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCgtgctggtgct                                                            <  1:1210020/150‑1 (MQ=255)
                                                                                             gCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGtgctggtgctgg                                                          >  1:1272752/1‑150 (MQ=255)
                                                                                             gCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGtgctggtgctgg                                                          >  2:770338/1‑150 (MQ=255)
                                                                                                cATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGcac                                                       >  1:1654915/1‑150 (MQ=255)
                                                                                                 atatCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGcaca                                                      <  1:1974846/150‑1 (MQ=255)
                                                                                                  tatCAGCAAGAAGCCGGGGATGCCACGCTCAACCATTTTCGTCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCacac                                                     <  2:851353/150‑1 (MQ=255)
                                                                                                    tCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGt                                                   <  2:13900/150‑1 (MQ=255)
                                                                                                      aGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTa                                                 <  2:3606466/150‑1 (MQ=255)
                                                                                                      aGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTa                                                 >  1:1060741/1‑150 (MQ=255)
                                                                                                       gCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTaa                                                >  2:2607348/1‑150 (MQ=255)
                                                                                                         aagaagCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAg                                              >  1:1096671/1‑150 (MQ=255)
                                                                                                         aagaagCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAg                                              >  1:1880671/1‑150 (MQ=255)
                                                                                                           gaagCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGaa                                            >  1:3417671/1‑150 (MQ=255)
                                                                                                            aagCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAAc                                           >  2:2654922/1‑150 (MQ=255)
                                                                                                                cGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAAAtggt                                       >  2:2214635/1‑150 (MQ=255)
                                                                                                                   ggATGCCCCGCTCAACCATTTTCGTCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGgc                                    <  2:327326/150‑1 (MQ=255)
                                                                                                                   ggATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTgg                                                                                                          <  1:2704958/80‑1 (MQ=255)
                                                                                                                   ggATGCAACGCTCAACTATTTTCGTCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGgc                                    <  1:3953989/150‑1 (MQ=255)
                                                                                                                       gCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCaa                                >  1:2595446/1‑150 (MQ=255)
                                                                                                                        ccACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCaaa                               >  1:4002886/1‑150 (MQ=255)
                                                                                                                        ccACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCaaa                               >  2:818778/1‑150 (MQ=255)
                                                                                                                         cACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCaaaa                              >  1:2143622/1‑150 (MQ=255)
                                                                                                                           cGCTCAACCATTTTCGTCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAAcc                            <  1:4110358/150‑1 (MQ=255)
                                                                                                                             cccAACCATTTTCGTCGTAAAAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCAt                          <  2:3211093/148‑1 (MQ=255)
                                                                                                                               cacccATTTTCGTCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGc                        <  1:951744/147‑1 (MQ=255)
                                                                                                                               cAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGc                        >  1:3704407/1‑150 (MQ=255)
                                                                                                                               cAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGc                        >  2:3015908/1‑150 (MQ=255)
                                                                                                                                   cATTTTCGTCGTAATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGCAAAg                    <  1:1476102/150‑1 (MQ=255)
                                                                                                                                    aTTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGCAAAGt                   >  2:1181811/1‑150 (MQ=255)
                                                                                                                                      tttCGTCGTAATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGCAAAGTAt                 <  1:3187153/150‑1 (MQ=255)
                                                                                                                                             gtcATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGCAAAGTATCAGgcgc          >  1:591082/1‑150 (MQ=255)
                                                                                                                                              tcATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGCAAAGTATCAGGCGCt         >  2:667012/1‑150 (MQ=255)
                                                                                                                                              tcATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGCAAAGTATCAGGCGCt         >  2:1851919/1‑150 (MQ=255)
                                                                                                                                                 tAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGCAAAGTATCAGGCGCTggg      <  1:115914/150‑1 (MQ=255)
                                                                                                                                                 tAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGCAAAGTATCAGGCGCTggg      <  1:1005292/150‑1 (MQ=255)
                                                                                                                                                  aaacCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGCAAAGTATCAGGCGCTgggg     <  2:2732544/150‑1 (MQ=255)
                                                                                                                                                   aaaCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGCAAAGTATCAGGCGCTGGGGc    >  2:2839005/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TAGCGGTCTAATAACATCCCTCAAGGGAAAAAGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGCAAAGTATCAGGCGCTGGGGCTG  >  AP012306/1854702‑1855000

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑