Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123063,182,8610AC27.7% 295.5 / 29.6 130L198F (TTA→TTC) yiaNpredicted transporter
Reads supporting (aligned to +/- strand):  ref base A (77/17);  new base C (0/36);  total (77/53)
Fisher's exact test for biased strand distribution p-value = 2.02e-19
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 205 total aligned reads displayed.

TTATTCCCCCTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGC‑TCCGGCCTGTTTACCCCT‑ACCGAAGCCGGGGCCGTTGCTGCTTTCTATGC  >  AP012306/3182713‑3183010
                                                                                                                                                    |                                                                                                                                                       
 tattCCCCCTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGGCTTTCCCCTGGCCTGATGGTTGGCGGCACGCTGATGTCTTCCTGGTGGGGGGCGGCCAGGCCGCCTAATTTatc                                                                                                                                                       >  2:734637/1‑148 (MQ=255)
    tCCCCCTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCg                                                                                                                                                    <  1:1679452/150‑1 (MQ=255)
     cccccTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCCTTGCCCCTGGCATGATGATGGGGGCAAAGCTGATGCTTTCCTGGGGGGGGCAGGGCAGGCGCCTTAATTTAtccccc                                                                                                                                                   >  2:590427/1‑148 (MQ=255)
       cccTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCa                                                                                                                                                 <  2:1812725/150‑1 (MQ=255)
        ccTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTTCCCCCGGCATGATGATGGGGGCCACGCTGATGCTTTCCTGGTGGGGGGCGGGCAGGCGCCTTTATTTACCCCGcccg                                                                                                                                                >  2:529876/1‑148 (MQ=255)
        ccTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCCTGCATGATGATGGGGGCAACCCCGATGCTTTCCTTGTGGTGGGAGGGCAGGGGCCTTAATTTTCcccccccc                                                                                                                                                >  2:195730/1‑148 (MQ=255)
        ccTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCCCGCCTGATGATGGGGGGAACGCTGATGCTTTCCTGGGGGGGGGGGGGCCGGCGCCTTTATTTTCcccccccg                                                                                                                                                >  2:879894/1‑148 (MQ=255)
          ttCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTTCCCCTGGGGTGATGATGGGGGCCACCCTGCTGCTTTCCTGGTGGTGGGGGGCCAGGCGCCCTTATTTTCCCCAcccccc                                                                                                                                              >  2:397232/1‑146 (MQ=255)
           tCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCCTTTTCCCCGGCGGGATTATGGGGGCAACGCCTATGCCTTCCTGGGGGTTGGGGGCCCCTCCCCCTTATTTATcccccccccaa                                                                                                                                             >  2:2382426/1‑145 (MQ=255)
                tCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTCCCTGGTGGTGGCAGGCCAGTCGCCTTAATTTCCCCCGCCAGCAAAAAGc                                                                                                                                        <  1:2327253/150‑1 (MQ=255)
                 ccGTTTATTATCTTCGGCGTTGCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCCCGCATTATGATGGGCGCAACGCTGATGCTTACCTGGTGGGGGCAGGGCAGTCGCCTTTATTTACCCCCCCCGCAAAAAGCa                                                                                                                                       >  2:165435/1‑150 (MQ=255)
                  cGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGGAGTGGCCCTGGGAGGGTGGGGGGGGGAAAGGGGGTGGGTAGCGGGGGGGGGGAGGGGGGGCGGCGTAATTTATCCCGCCAGCAAAAAGaaa                                                                                                                                      >  2:4004461/1‑150 (MQ=255)
                       attatCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTTCCCCTGGCATGATGATGGGGGCAACGCCGATGCTTACCTGGGGGTGGCAGGCCAGTCGCCTTTATTTAACCCCCCCGCAAAAAACAACAAta                                                                                                                                 >  2:90709/1‑149 (MQ=255)
                       attatCTTCGGCGTTTCCAGCGAATTTTCCCCCAGAAAGCTTTTTTGGGGGGGCATTGCCCCGGCCATGATGAGGGGCGCAACGCTGATGCTTCCCTGGTGGTGGCAGGCCAGTCCCCTTAATTTCCCCCGCCAGCAAAAAGCAACGATg                                                                                                                                 <  2:4074733/150‑1 (MQ=255)
                          atCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGGGATGGCCCCGGCGTGATGGTGGGGGCGACGCTGATGCTTACCCGGTGGGGGGAGGCCAGGCGCCTTTATTTTTCCCCCCCGCAAAAAACAACGATGAAg                                                                                                                              >  2:2815507/1‑150 (MQ=255)
                          atCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTTCCCCCCGGCTGGTGGTGGGGGCAACCCTGATGTTTATCTGGGGGGGGCCGGCCAGTCGCCTTAATTTTTCCCGCCCCCAAAAAAAAAAAATGCAg                                                                                                                              >  2:481522/1‑150 (MQ=255)
                           tCTTCGGCGTTTCCAGCGGATTACCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGAGGGGCGCAACGCTGATGCTTACCGGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAgg                                                                                                                             <  1:1128488/150‑1 (MQ=255)
                                 gCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCCATGCCCCCGGCCTGATGATGGGCGCAACGCCGATGCTTACCCGGTGGGGGGAGGCCAGTCGCCCTAATTTACCCCCCCCGCAAAAAGCAACGATGCAGGAAAACt                                                                                                                       >  2:3885997/1‑150 (MQ=255)
                                 gCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGACGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCt                                                                                                                       <  1:249839/150‑1 (MQ=255)
                                    tttCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGc                                                                                                                    <  2:3728965/150‑1 (MQ=255)
                                      tCCAGCGGATTATCCATCAGAAAGCTGTTTAGGGGGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGCTGCTTACCTGGGGGGGGCGGGCCAGCCGCCTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCAc                                                                                                                  <  2:67495/150‑1 (MQ=255)
                                        cAGCGGATTATCCATCAGTAAGCTGTTTATGGGGGGCATGGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCActc                                                                                                                <  1:3787820/150‑1 (MQ=255)
                                         ggCGGATTACCCATCAGTAGGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCGGGCCAGTCGCTTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCActct                                                                                                               <  2:2970366/149‑1 (MQ=255)
                                         aGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCCTTGCCCCCCGCATGATGATGGGGGCAACGCTGATGCTTACCTCGGGGGGGGGGGCCACGCGCCTTTATTTACCCCCCCCGCAAAAAACAACGAAGCAGGAAAACTGGGAccct                                                                                                               >  2:322329/1‑150 (MQ=255)
                                                 aTCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTATTTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGtctc                                                                                                       <  1:78187/150‑1 (MQ=255)
                                                  cccATCAGTAGGCTTTTTAGGGCGGGCTTGGCCCCTGCCATGATGAGGGGCGCACCGCTGATGTTTCCCGGGGGGGGCCGGGCCAGTCCCTTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTcc                                                                                                      <  2:3072263/149‑1 (MQ=255)
                                                   ccATCAGTAAGTTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTCCCTGGGGGTGGCAGGCCAGTCGCTTTAATTCCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCg                                                                                                     <  2:1544964/150‑1 (MQ=255)
                                                   caaTCAGTAAGTTTTTTAGGGCGGGCATTGCCCTTGGCATGATGAGGGGCGCACCGCTGATGTTTACCGGGTGGTGGCGGGCCAGTCCCCTTATTTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCg                                                                                                     <  2:3094889/148‑1 (MQ=255)
                                                         gTAAGTTTTTTATGGCGGGCTTTGCCCCTGGCATGATGAGGGGCGCAACGTTGATGCTTCCCTGGTGGGGGCAGGCCAGTCGCCTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCt                                                                                               <  2:2889165/150‑1 (MQ=255)
                                                               tGTTTATGGCAGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGgcgc                                                                                         >  1:1084649/1‑150 (MQ=255)
                                                                       gCGGCCTTTCCCCTTGGCATGATGTGGGGCGCACCGCTGAGCTTTACCTGGTGGTGGCAGGCCAGTCGCTTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTttctt                                                                                 <  2:2848899/150‑1 (MQ=255)
                                                                        cGGGCATTGCCCTTGGCATGATGAGGGGCGCAACGCTGATGCTTACCTGGGGGGGGCAGGCCAGTCCCTTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTttcttc                                                                                <  2:2735114/150‑1 (MQ=255)
                                                                        cGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTttcttc                                                                                >  1:1339995/1‑150 (MQ=255)
                                                                        cGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTttcttc                                                                                >  2:3687518/1‑150 (MQ=255)
                                                                         gggCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTcc                                                                               >  2:1338361/1‑150 (MQ=255)
                                                                         gggCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTcc                                                                               >  2:1636730/1‑150 (MQ=255)
                                                                            cATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGt                                                                            <  1:2861515/150‑1 (MQ=255)
                                                                            cATTCCCCCTGGTGTGATGAGGGGCGCACCGCTGATCTTTCCCGGGGGGTGGCAGCCACCTCTCTTTAATTCCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGt                                                                            <  2:2019920/150‑1 (MQ=255)
                                                                             aTTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGtc                                                                           >  2:2996100/1‑150 (MQ=255)
                                                                             aTTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGtc                                                                           >  2:1913586/1‑150 (MQ=255)
                                                                             aTTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGtc                                                                           >  2:1203570/1‑150 (MQ=255)
                                                                                gCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTCCCTGGTGGTGGCGGGCCAGTCGCTTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGtcatc                                                                        <  2:771515/150‑1 (MQ=255)
                                                                                 ccccTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGGGGGGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGtcatca                                                                       <  1:3206872/150‑1 (MQ=255)
                                                                                   ccTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCatt                                                                     <  1:5187/150‑1 (MQ=255)
                                                                                     tGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCattat                                                                   >  1:4072311/1‑150 (MQ=255)
                                                                                     tGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCattat                                                                   >  2:515491/1‑150 (MQ=255)
                                                                                     tGGCAGGAGGATGGGCGCAACGCTGATGCTTCCCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCattat                                                                   <  1:277879/150‑1 (MQ=255)
                                                                                       gaatgatgatgGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTATTTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTg                                                                 <  1:4030671/148‑1 (MQ=255)
                                                                                       gCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTg                                                                 >  1:3861742/1‑150 (MQ=255)
                                                                                          tgatgatgGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCg                                                              >  2:2079041/1‑150 (MQ=255)
                                                                                           gatgatgGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCgg                                                             >  2:3161380/1‑150 (MQ=255)
                                                                                           gatgatgGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCgg                                                             >  2:3606763/1‑150 (MQ=255)
                                                                                             tgatgGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCgggg                                                           >  1:619664/1‑149 (MQ=255)
                                                                                             tgatgGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGt                                                           >  2:3977925/1‑150 (MQ=255)
                                                                                               atgGGCGCAACGTTGATGTTTCCCTGGTGGTGACAGGCCAGTCGCCTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGttt                                                         <  2:2619939/150‑1 (MQ=255)
                                                                                               atgGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGttt                                                         <  1:3395904/150‑1 (MQ=255)
                                                                                                tgGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGcttt                                                        >  1:192202/1‑149 (MQ=255)
                                                                                                ggggCGCACCGCTGATGCTTACCGGGTGGTGGCAGGCCAGTCGCCTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTc                                                        <  2:619664/149‑1 (MQ=255)
                                                                                                 gggCGAACCGCTGATGCTTCCGTGGTGGTGACAGGCCAGTCCCTTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCg                                                       <  1:3293710/150‑1 (MQ=255)
                                                                                                   gcgcAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGC‑t                                                    <  2:1219222/150‑1 (MQ=255)
                                                                                                      cacctttGATGCTGACCTGGGGGTGGCAGGCCGGCCGCTTTATTTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGC‑TCCg                                                 <  2:916687/144‑1 (MQ=255)
                                                                                                       aaCGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGC‑TCCgg                                                >  2:1735605/1‑150 (MQ=255)
                                                                                                        aCGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTCGC‑TCCggg                                               >  1:3579636/1‑149 (MQ=255)
                                                                                                          gCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTGC‑TCCCGccc                                             >  1:3268389/1‑149 (MQ=255)
                                                                                                            tGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTTC‑TCCCGCCCGt                                           >  2:2593381/1‑150 (MQ=255)
                                                                                                            tGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTTC‑TCCCGCCCGt                                           >  1:4141006/1‑150 (MQ=255)
                                                                                                              aTGCTTCCCTGGTGGGGGCGGCCCCGCCCCCTTATTTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGC‑TCCGGCCTGttt                                         <  2:269164/150‑1 (MQ=255)
                                                                                                              aTGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTTTC‑TCCCGccgggtt                                         >  2:2554138/1‑145 (MQ=255)
                                                                                                               tGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTTC‑TCCCGCCCGtttt                                        >  1:4125354/1‑149 (MQ=255)
                                                                                                                gCTTACTGGGTGGGGGCGGCCCAGTCGCTTTATTTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGC‑TCCGGCCTGTTTAc                                       <  1:2013987/150‑1 (MQ=255)
                                                                                                                gCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTGCTTCCGCCCGGtttt                                        >  2:2391874/1‑149 (MQ=255)
                                                                                                                 cttccgtggtggtggCGGGCCAGTCGCCTTATTTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGC‑TCCGGCCTGTTTAcc                                      <  2:1114340/144‑1 (MQ=255)
                                                                                                                 cTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGC‑TCCGGCCTGTTTAcc                                      <  1:3481143/150‑1 (MQ=255)
                                                                                                                    aCCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTGC‑TCCGGCCTGTTTTTCCCt                                   >  1:309524/1‑150 (MQ=255)
                                                                                                                    aCCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTGC‑TCCCGCCCGtttttcccc                                   >  2:2289952/1‑144 (MQ=255)
                                                                                                                     ccTGGTGGTGGCAGGCCAGTCGCCTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGC‑TCCGGCCTGTTTACCCCT‑a                                 <  1:189339/150‑1 (MQ=255)
                                                                                                                      tgggtgggggCAGGCCAGTCGCTTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGC‑TCCGGCCTGTTTACCCCT‑Ac                                <  1:3828498/148‑1 (MQ=255)
                                                                                                                       tggtggtggCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTGTGC‑TCCCGGCTGCTTTCCCCC‑Acc                               >  2:2238646/1‑150 (MQ=255)
                                                                                                                       gggggggggCAGGCCAGTCGCTTTATTTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGC‑TCCGGCCTGTTTACCCCT‑Acc                               <  2:1999630/143‑1 (MQ=255)
                                                                                                                        ggtggtggCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTCGC‑TCCCGCCCGTTTTtccct‑cccc                              >  1:3016784/1‑149 (MQ=255)
                                                                                                                          tggtggCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTGC‑TCCGGCCCGTTTTCCCCT‑ACCCaa                            >  1:1890771/1‑150 (MQ=255)
                                                                                                                          tggtggCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTCGC‑TCCCGCCTGTTTACCCCC‑Acccac                            >  1:1633823/1‑147 (MQ=255)
                                                                                                                              ggCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGCGTTTTC‑TCCGGCCCGTTTTCCCCT‑ACCCAAAccc                        >  1:647955/1‑149 (MQ=255)
                                                                                                                                cAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTTGC‑TCCCGCCCGTTTTCCCCC‑CCCCAAGCCggg                      >  2:1276560/1‑150 (MQ=255)
                                                                                                                                cAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTGC‑TCCTGGCCCTTTTCCCCT‑ACCCCAGCCggg                      >  2:3850467/1‑150 (MQ=255)
                                                                                                                                 aGGCCAGTCGCCTTATTTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGC‑TCCGGCCTGTTTACCCCT‑ACCGAAGCCgggg                     <  1:2408960/150‑1 (MQ=255)
                                                                                                                                  ggCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTGC‑TCCCGCCTGTTTTTCCCC‑ACCCAAGGCggggg                    >  2:3462432/1‑149 (MQ=255)
                                                                                                                                     cAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTGC‑TCCCGCCCGTTTTACCCT‑ACCCCAGCCCGGGcccg                 >  1:3900500/1‑148 (MQ=255)
                                                                                                                                     cAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTCGC‑TCCCGCCCGTTTTCCCCT‑TCCCCAACCGGGGCCGt                 >  1:378191/1‑150 (MQ=255)
                                                                                                                                      aGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTGC‑TCCCGCCCGTTTTTCCCTCCCCCAACCCGGGGcgcg                 >  2:1036040/1‑147 (MQ=255)
                                                                                                                                          gctttATTTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTAGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGC‑TCCGGCCTGTTTACCCCT‑ACCGAAGCCGGGGCCGTtgctg            <  1:2311090/147‑1 (MQ=255)
                                                                                                                                          gcgttAATTGCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGC‑TCCGGCCTGTTTACCCCT‑ACCGAAGCCGGGGCCGTtgctg            <  1:3974121/147‑1 (MQ=255)
                                                                                                                                           ccTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTCGC‑TCCCGCCCTTTTTCCCCT‑ACCGAAGCCCGGGCCGGtgctgc           >  1:264855/1‑150 (MQ=255)
                                                                                                                                            cTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGC‑TCCGGCCTGTTTACCCCT‑ACCGAAGCCGGGGCCGTTgctgct          <  1:2447940/150‑1 (MQ=255)
                                                                                                                                            cTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGC‑TCCGGCCTGTTTACCCCT‑ACCGAAGCCGGGGCCGTTgctgct          <  1:1243548/150‑1 (MQ=255)
                                                                                                                                             ttAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTTTC‑TCCGGCCCGTTTTCACCC‑CCCGCAGCCCGGGCCGTTTCTGCtt         >  1:1484678/1‑150 (MQ=255)
                                                                                                                                             ttAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTGC‑TCCCGCCCGTTTTTCCCC‑ACCCAAGCCCGGGCCGCTGCTGCtt         >  1:810396/1‑150 (MQ=255)
                                                                                                                                              tAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTGC‑TCCCGCCCGTTTTCCCCT‑ACCCAAACCCGGGCCGTTGCTGCttt        >  1:433230/1‑150 (MQ=255)
                                                                                                                                               gaTTTACTCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGC‑TCCGGCCTGTTTACCCCT‑ACCGAAGCCGGGGCCGTTGCTGCTTTc       <  2:3391547/149‑1 (MQ=255)
                                                                                                                                                   tACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTGC‑TCCCGCCCGTTTTCCCCC‑ACCCAAGCCCGGGGCGCTTCTGCtttttttg   >  1:1877530/1‑145 (MQ=255)
                                                                                                                                                    |                                                                                                                                                       
TTATTCCCCCTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGC‑TCCGGCCTGTTTACCCCT‑ACCGAAGCCGGGGCCGTTGCTGCTTTCTATGC  >  AP012306/3182713‑3183010

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑