Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123063,698,8280TG21.1% 725.3 / 16.0 251F68V (TTT→GTT) aphAacid phosphatase/phosphotransferase, class B, non‑specific
Reads supporting (aligned to +/- strand):  ref base T (17/181);  new base G (53/0);  total (70/181)
Fisher's exact test for biased strand distribution p-value = 8.22e-40
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 313 total aligned reads displayed.

ACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAACAATGGCTGGGATGAATTCAGCATTCCAAAAGAG  >  AP012306/3698679‑3698977
                                                                                                                                                     |                                                                                                                                                     
aCAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGt                                                                                                                                                       <  2:1863938/150‑1 (MQ=255)
  aGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGttt                                                                                                                                                     <  1:3165188/150‑1 (MQ=255)
  aGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGttt                                                                                                                                                     <  2:1199854/150‑1 (MQ=255)
   gTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTg                                                                                                                                                    <  2:808498/150‑1 (MQ=255)
     tCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGGTTggt                                                                                                                                                  >  2:4154468/1‑148 (MQ=255)
      ccGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGata                                                                                                                                                 <  1:1670845/150‑1 (MQ=255)
        gCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATc                                                                                                                                               <  1:2596875/150‑1 (MQ=255)
         cTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCg                                                                                                                                              <  2:874371/150‑1 (MQ=255)
         cTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGGTTTATATCg                                                                                                                                              >  1:1299733/1‑150 (MQ=255)
         cTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGGTTGATATCg                                                                                                                                              >  2:3481940/1‑150 (MQ=255)
          tGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGGTTGATATCGa                                                                                                                                             >  1:1252550/1‑150 (MQ=255)
           gTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGGTTGATATCGAt                                                                                                                                            >  1:3708454/1‑150 (MQ=255)
              gCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGac                                                                                                                                         <  1:809192/150‑1 (MQ=255)
              gCCCGGGCCTTCACTCCTTCACCCCTTAACCCTTGGACTAACGTTGCCAGGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGac                                                                                                                                         <  2:662822/150‑1 (MQ=255)
                ccTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGGTTGATATCGATGacac                                                                                                                                       >  1:2962477/1‑150 (MQ=255)
                 cTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACg                                                                                                                                      <  1:1719027/150‑1 (MQ=255)
                   ggCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGt                                                                                                                                    <  1:1809940/150‑1 (MQ=255)
                      cTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACt                                                                                                                                 <  1:1400473/150‑1 (MQ=255)
                        cATCTCCTTCACCGCTTAACCCTGGGACTAACTTTGCCAGGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACttt                                                                                                                               <  2:3885582/150‑1 (MQ=255)
                        cATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGGTTGATATCGATGACACGGTACttt                                                                                                                               >  2:2982818/1‑150 (MQ=255)
                        cATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGGTTGATATCGATGACACGGTACttt                                                                                                                               >  2:307968/1‑150 (MQ=255)
                          tctcCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGGTTTATATCGATGACACGGGACttttt                                                                                                                             >  2:365911/1‑150 (MQ=255)
                          tctcCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGGTTGATATCGATGACACGGTACttttt                                                                                                                             >  1:1450904/1‑150 (MQ=255)
                                tCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGt                                                                                                                       <  2:2341215/150‑1 (MQ=255)
                                 cACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTc                                                                                                                      <  1:2313149/150‑1 (MQ=255)
                                      cTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGc                                                                                                                 <  1:2723233/150‑1 (MQ=255)
                                       ttAACCCTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCt                                                                                                                <  2:1564980/150‑1 (MQ=255)
                                         aaCCCTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTc                                                                                                              <  2:3365870/150‑1 (MQ=255)
                                          aCCCTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCt                                                                                                             >  1:519797/1‑150 (MQ=255)
                                          aCCCTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGGTTGATATCGATGACACGGTACTTTTTTCCCGTCCGGGCTTCt                                                                                                             >  2:2171142/1‑150 (MQ=255)
                                           cccTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTg                                                                                                            <  2:1890091/150‑1 (MQ=255)
                                            ccTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTgg                                                                                                           <  2:3554676/150‑1 (MQ=255)
                                            ccTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGGTTGATATCGATGACACGGTACTTTTTTTCAGTCCGGGCTTCttg                                                                                                           >  1:403649/1‑148 (MQ=255)
                                            ccTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGCGGGGGTTTATATCGAGGGCACGGTACCTTTTTCCAGCACGGGCCTCTgg                                                                                                           >  2:4008725/1‑150 (MQ=255)
                                              tGGGACTAACGTTGCCAGGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGgcg                                                                                                         <  2:1265306/150‑1 (MQ=255)
                                                ggACTAACGTTGCCAGGCTTGCTGAACAGGCCCCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGcgcg                                                                                                       <  2:3783323/150‑1 (MQ=255)
                                                 gtcTAACGTTGCCAGGCTTGCTAACCGGTCACCCACTGATTGGTTTTAGGTCGCACAAATTAAAAATAACCTCGCAGGGCTTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCgg                                                                                                      <  2:3335391/148‑1 (MQ=255)
                                                  aCTAACGTTGCCAGGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGc                                                                                                     <  1:4090500/150‑1 (MQ=255)
                                                   cTAACGTTGCCAGGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGGTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCa                                                                                                    >  2:1064170/1‑150 (MQ=255)
                                                     aaCGTTGCCAGGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCaaa                                                                                                  <  1:2236117/150‑1 (MQ=255)
                                                       cGTTGCCAGGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCaaaaa                                                                                                <  1:235318/150‑1 (MQ=255)
                                                        gTTGCCAGGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGGTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCaaaaaa                                                                                               >  1:3236717/1‑150 (MQ=255)
                                                          tGCCAGGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCAAAAAAAc                                                                                             <  2:780876/150‑1 (MQ=255)
                                                            ccAGGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCAAAAAAACCt                                                                                           <  2:1396611/150‑1 (MQ=255)
                                                             cAGGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCAAAAAAACCtt                                                                                          <  2:1474777/150‑1 (MQ=255)
                                                             cAGGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCAAAAAAACCtt                                                                                          <  2:2541987/150‑1 (MQ=255)
                                                              aGGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGGTTGATATCGATGACACGGGACTTTTTTCCCGTCCCGGCTTCTGGCGCGGCaaaaaaaacctc                                                                                         >  2:925233/1‑145 (MQ=255)
                                                               ggCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCAAAAAAACCTtct                                                                                        <  1:4058636/150‑1 (MQ=255)
                                                                gCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGGTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCAAAAAAACCTtctc                                                                                       >  2:3610835/1‑150 (MQ=255)
                                                                  ttGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCAAAAAAACCTTCTCGc                                                                                     <  2:448060/150‑1 (MQ=255)
                                                                   tGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCAAAAAAACCTTCTCGcc                                                                                    <  1:2364527/150‑1 (MQ=255)
                                                                        aacggccACCCATTCATGGGGTTTCGGTCGCAAAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCAAAAAAACCTTCTCGCCAGaaa                                                                               <  2:4148437/144‑1 (MQ=255)
                                                                        aaCAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCAAAAAAACCTTCTCGCCAGaaa                                                                               <  1:3851444/150‑1 (MQ=255)
                                                                         aCAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAg                                                                              <  1:2536936/150‑1 (MQ=255)
                                                                          cAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGGACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCAAAAAAAACTTCTCGCCCGaaaac                                                                             >  1:3582953/1‑148 (MQ=255)
                                                                              cACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGGGGGGGGTGGGATTGATGGAACGGGAATTTTTTTCAGGGCGGGGTTCTGGCGCGGGAAAAAAAAAATTTCGCCAGAAAAGGAAg                                                                         >  2:1991249/1‑150 (MQ=255)
                                                                                cccATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGAt                                                                       <  1:664244/150‑1 (MQ=255)
                                                                                   attcattGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTAt                                                                    <  2:3259779/150‑1 (MQ=255)
                                                                                     tcattGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCt                                                                  <  2:2117386/150‑1 (MQ=255)
                                                                                      cattGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGGTATCGGAGACACGGTACTTTTTTTCAGTCCGGGCTTCTGGCGCGGGCAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTg                                                                 >  2:558705/1‑150 (MQ=255)
                                                                                        ttGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGaa                                                               <  1:3785889/150‑1 (MQ=255)
                                                                                        ttGGGTTTCGGTCGCACAAATTAAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGaa                                                               <  2:2674453/150‑1 (MQ=255)
                                                                                            gTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAAt                                                           <  1:998089/150‑1 (MQ=255)
                                                                                            gTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAAt                                                           <  1:2503848/150‑1 (MQ=255)
                                                                                            gTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAAt                                                           <  1:699000/150‑1 (MQ=255)
                                                                                               tCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCt                                                        <  1:1273117/150‑1 (MQ=255)
                                                                                               tCGGTCCCACAAATTGAAAATGGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCt                                                        <  2:1968248/150‑1 (MQ=255)
                                                                                                   tCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTgtgt                                                    <  1:1961263/150‑1 (MQ=255)
                                                                                                   tCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGGTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTgtgt                                                    >  1:1059389/1‑150 (MQ=255)
                                                                                                    cGCACAAATTGAAAATAGCCTCGCAGGTCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGtt                                                   <  2:2924342/150‑1 (MQ=255)
                                                                                                     gCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTc                                                  <  2:2786528/150‑1 (MQ=255)
                                                                                                      cacaAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGGTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCt                                                 >  1:2156430/1‑150 (MQ=255)
                                                                                                      cacaAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGGTTGATATCGATGACACGGTACTTTTTTCCAGTCCCGGCTTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAAACCTGTGTTCt                                                 >  1:1845022/1‑150 (MQ=255)
                                                                                                       acaAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGGTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTg                                                >  1:3511775/1‑150 (MQ=255)
                                                                                                         aaaTTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTggg                                              <  2:758823/150‑1 (MQ=255)
                                                                                                          aaTTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGa                                             <  1:2629669/150‑1 (MQ=255)
                                                                                                           aTTGAAAATAGCCTCGCAGGGCTTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGaa                                            <  2:2379169/150‑1 (MQ=255)
                                                                                                           aTTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGGTTGATATCGATGACACGGTACTTTTTTCCAGTCCCGGCTTCTGGCGCGACAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTgggga                                            >  1:2850697/1‑148 (MQ=255)
                                                                                                                 aaTAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATg                                      <  1:27016/150‑1 (MQ=255)
                                                                                                                  aTAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGGTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAAACCTGTGTTCTGGGAAAAAATGa                                     >  2:1600776/1‑150 (MQ=255)
                                                                                                                    aGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAAc                                   <  2:727411/150‑1 (MQ=255)
                                                                                                                     gCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGGTTGATATCGATGACACGGTACTTTTTTCCAGGCCGGGCTTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGGAAAAATGAACa                                  >  1:2417/1‑150 (MQ=255)
                                                                                                                      ccTCGCAGGGCGTCCGCCAATGGCGGTGGGGGTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCAAAAAAAACTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAACaa                                 >  2:2269654/1‑150 (MQ=255)
                                                                                                                         cGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAACAATgg                              <  1:60380/150‑1 (MQ=255)
                                                                                                                           cAGGGCGTCCGCCAATGGCGGTGGGGGTTGGTATCAATGAAACGGGACTTTTTTCCCGTCCGGGGTTCCCGGGGGGCAAAAAAACCTTCTCGCCAGAAAACGAAGAGTATCTGAAAAATCCTGTGTTTTTGGAAAAAATGAACaaaagcg                            >  2:1331228/1‑145 (MQ=255)
                                                                                                                              ggCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAACAATGGCTggg                         <  1:3379910/150‑1 (MQ=255)
                                                                                                                              ggCGACCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCCGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAACAATGGCTggg                         <  1:3455378/150‑1 (MQ=255)
                                                                                                                               gCGTCCGCCAATGGCGGTGGGGGTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAACAATGGCTGGGa                        >  1:3671193/1‑150 (MQ=255)
                                                                                                                                cGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAACAATGGCTGGGAt                       <  1:2797766/150‑1 (MQ=255)
                                                                                                                                cGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAACAATGGCTGGGAt                       <  1:2006926/150‑1 (MQ=255)
                                                                                                                                    cGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAACAATGGCTGGGATGAAt                   <  1:3080985/150‑1 (MQ=255)
                                                                                                                                      ccAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAACAATGGCTGGGATGAATTc                 <  2:2424169/150‑1 (MQ=255)
                                                                                                                                       cAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAACAATGGCTGGGATGAATTCa                <  2:3213089/150‑1 (MQ=255)
                                                                                                                                         aTGGCGGTGGGGGTTGATATCGATGACACGGGACTTTTTTTCAGTCCGGGCTTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTTAAAAATCCTGTGTTCTGGGAAAAAAAGAACAAAGGCTGGGGTGGATTCAGc              >  2:2689021/1‑150 (MQ=255)
                                                                                                                                          tGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAACAATGGCTGGGATGAATTCAGCa             >  2:1141945/1‑150 (MQ=255)
                                                                                                                                           ggCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAACAATGGCTGGGATGAATTCAGCAt            <  1:3964663/150‑1 (MQ=255)
                                                                                                                                            gCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAACAATGGCTGGGATGAATTCAGCAtt           <  1:1212395/150‑1 (MQ=255)
                                                                                                                                                tGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAACAATGGCTGGGATGAATTCAGCATTCCaa       <  2:484076/150‑1 (MQ=255)
                                                                                                                                                   ggTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAACAATGGCTGGGATGAATTCAGCATTCCAAAag    >  1:3905522/1‑150 (MQ=255)
                                                                                                                                                     tttGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAACAATGGCTGGGATGAATTCAGCATTCCAAAagag  <  1:1011967/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAACAATGGCTGGGATGAATTCAGCATTCCAAAAGAG  >  AP012306/3698679‑3698977

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑