Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP012306935,0540GT24.7% 267.3 / 36.0 93intergenic (+24/‑1406)putP/ycdOproline:sodium symporter/conserved protein
Reads supporting (aligned to +/- strand):  ref base G (13/57);  new base T (23/0);  total (36/57)
Fisher's exact test for biased strand distribution p-value = 6.19e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 153 total aligned reads displayed.

GGCAGTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTG‑TTT‑GGCTTCAGCA‑G‑CGGGTTGCGCT‑CCCTTAATGT‑GCCTCGCCATATAAATTGAA‑TGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGT‑CCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGCCACCAA  >  AP012306/934905‑935201
                                                                                                                                                      |                                                                                                                                                            
ggCAGTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTtttg                                                                                                                                                              >  1:3265489/1‑150 (MQ=255)
 gCAGTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGGtttg‑t                                                                                                                                                            >  2:2548404/1‑150 (MQ=255)
  cAGTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTtttg‑tt                                                                                                                                                           <  1:2672971/150‑1 (MQ=255)
   aGTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGttttt‑ttt                                                                                                                                                          >  1:3419235/1‑150 (MQ=255)
   aGTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGgtttg‑ttt                                                                                                                                                          >  2:687583/1‑150 (MQ=255)
   aGTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGGgttg‑ttt                                                                                                                                                          >  2:2023252/1‑150 (MQ=255)
   aGTATTGGGATTGTAGTGTTTAGTTTGCGGGGTAAGGCGCGGTCAGCGGGGATGCAAAACCGCTTTGCCGAGGCCGATGCGCACTATCTTTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTtttg‑ttt                                                                                                                                                          <  2:3973447/150‑1 (MQ=255)
     tATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTT‑TTT‑gg                                                                                                                                                       >  1:541788/1‑150 (MQ=255)
     tATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTG‑TTT‑gg                                                                                                                                                       <  1:3341766/150‑1 (MQ=255)
      aTTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGGTTTTT‑TTT‑gg                                                                                                                                                       >  1:3981045/1‑150 (MQ=255)
         gggATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTT‑TTT‑TGGTTc                                                                                                                                                   >  1:1114477/1‑150 (MQ=255)
         gggATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTG‑TTT‑GGCTTc                                                                                                                                                   <  1:2050138/150‑1 (MQ=255)
         gggATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGGTTTT‑TTT‑TGCTTc                                                                                                                                                   >  2:3352509/1‑150 (MQ=255)
          ggATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTG‑TTT‑GGCTTca                                                                                                                                                  <  2:902251/150‑1 (MQ=255)
          ggATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTG‑TTT‑GGCTTca                                                                                                                                                  <  1:1896687/150‑1 (MQ=255)
          ggATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGGtttt‑tttttgttct                                                                                                                                                   >  2:3063804/1‑143 (MQ=255)
           gATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGAAGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTT‑TTT‑TGCTTccg                                                                                                                                                 >  1:1014772/1‑148 (MQ=255)
            aTTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGATTTT‑TTT‑TGCtttcgc                                                                                                                                                >  1:1398469/1‑146 (MQ=255)
             ttGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTG‑TTT‑GGCTTcagca                                                                                                                                               <  2:487465/150‑1 (MQ=255)
             ttGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGGTTTT‑TTT‑TGGTTctgca                                                                                                                                               >  2:3914215/1‑150 (MQ=255)
              tGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTG‑TTT‑TGGTTcagca‑g                                                                                                                                             >  2:3760602/1‑150 (MQ=255)
               gTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTT‑TTT‑TGCTTCagca‑g‑c                                                                                                                                           >  1:339545/1‑150 (MQ=255)
                 aGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTG‑TTT‑GGCTTCAGCA‑G‑Cgg                                                                                                                                         <  1:392204/150‑1 (MQ=255)
                  gtgtTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTT‑TTT‑TGCTTCAGCA‑G‑Cggg                                                                                                                                        >  1:1692147/1‑150 (MQ=255)
                   tgtTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTG‑TTT‑TGCTTCCGCA‑G‑Cgggg                                                                                                                                       >  1:3109192/1‑149 (MQ=255)
                      ttAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTG‑TTT‑GGCTTCAGCA‑G‑CGGGTTgc                                                                                                                                    <  2:514717/150‑1 (MQ=255)
                       tAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTT‑TTT‑TGGTTCTCCA‑G‑CGGGTTtcg                                                                                                                                   >  1:1531669/1‑150 (MQ=255)
                        aGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTG‑TTT‑TGCTTCAGCC‑A‑CCGGGTgcgc                                                                                                                                  >  1:1841179/1‑150 (MQ=255)
                        aGTTTGCTGGGTAAAGCGCAGTCAGCGGCGATGAAAAAACGCTTTGCCGAGGCCGATGAGCACTATAATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTG‑TTT‑GGCTTCAGCA‑G‑CGGGTTgcgc                                                                                                                                  <  2:2848196/150‑1 (MQ=255)
                         gTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTT‑TTT‑TGCTTCAGCA‑G‑CGGGGTGCGCt                                                                                                                                 >  1:1091197/1‑150 (MQ=255)
                         gTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTG‑TTT‑TGGTTCAGCA‑A‑CGGGGTGCGCt                                                                                                                                 >  1:3851011/1‑150 (MQ=255)
                          tttGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTT‑TTT‑TGCTTTAGCA‑G‑CGGGTTGCGCT‑c                                                                                                                               >  1:2408998/1‑150 (MQ=255)
                            tGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTT‑TTT‑TGCTTTAGCG‑G‑CGGGTTGTGCT‑ccc                                                                                                                             >  2:2181439/1‑150 (MQ=255)
                              cTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTG‑TTT‑GGCTTCAGCA‑G‑CGGGTTGCGCT‑CCCtt                                                                                                                           <  1:3863193/150‑1 (MQ=255)
                               tGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTG‑TTT‑CGCTTCCGCC‑G‑CGGGGTTCGCT‑CCCttt                                                                                                                          >  1:3039701/1‑149 (MQ=255)
                                 ggTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTT‑TTT‑TGCTTTTGCA‑GCCGGGTTG‑GCT‑ccccttat                                                                                                                        >  2:1957100/1‑145 (MQ=255)
                                 ggTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGGTTTGTTTT‑GGCTTC‑GCA‑G‑CGGGGTGCGCG‑cccctcat                                                                                                                        >  2:3868497/1‑145 (MQ=255)
                                  gTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTG‑TTT‑GGCTTCAGCA‑G‑CGGGTTGCGCT‑CCCTTAAtg                                                                                                                       <  2:1708613/150‑1 (MQ=255)
                                   tAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGGTTTT‑TTT‑GGCTTCACCA‑G‑CGGGTTTCGCT‑CCCCTAAttt                                                                                                                      >  1:3526064/1‑148 (MQ=255)
                                    aaaGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTG‑TTT‑TGCTTCAGCA‑G‑CGGGGTTCGCC‑CCCCTTAtgt‑g                                                                                                                    >  1:3274596/1‑150 (MQ=255)
                                     aaGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTT‑TTT‑TGCTTCAGCC‑G‑CGGGGTGCGCC‑CCCCTTATTT‑Gc                                                                                                                   >  1:368018/1‑150 (MQ=255)
                                     aaGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGATTTG‑TTT‑TGGTTTCGCA‑G‑CGGGTTGGGCT‑CCCCTTATGT‑Gc                                                                                                                   >  2:2318384/1‑150 (MQ=255)
                                      aGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTG‑TTT‑GGGTTCAGCA‑G‑CGGGGTGCGGC‑CCCCTaaattggc                                                                                                                   >  2:2019445/1‑144 (MQ=255)
                                      aGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTG‑TTT‑GGCTTCAGCA‑G‑CGGGTTGCGCT‑CCCTTAATGT‑Gcc                                                                                                                  <  1:3432441/150‑1 (MQ=255)
                                        cgcCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTT‑TTT‑TGGTTTAGCC‑G‑CCGGGTGCGCT‑CCCCTTATGT‑GGCTc                                                                                                                >  1:2830174/1‑150 (MQ=255)
                                        cgcCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTG‑TTT‑GGCTTCAGCA‑G‑CGGGTTGCGCT‑CCCTTAATGT‑GCCTc                                                                                                                <  1:2493598/150‑1 (MQ=255)
                                         gcCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTG‑TTT‑GGCTTCAGCA‑G‑CGGGTTGCGCT‑CCCTTAATGT‑GCCTCg                                                                                                               <  2:534651/150‑1 (MQ=255)
                                         gcCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGGTTTG‑TTT‑GGCGTCAGCA‑G‑CGGGGTGCGCC‑CCCCTAAtggggcccg                                                                                                                >  1:360378/1‑143 (MQ=255)
                                          ccGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTT‑TTT‑GGCTTCAGCA‑G‑CGGGGTGCGCT‑CCCTTAATGT‑GCCTCGc                                                                                                              >  1:2179411/1‑150 (MQ=255)
                                            gTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTG‑TTT‑TGCTTCATCA‑G‑CGGGGTGGGCC‑CCCCTTATGT‑GCCCCGccc                                                                                                            >  2:3156755/1‑149 (MQ=255)
                                            gTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTG‑TTT‑GGCTTCAGCA‑G‑CGGGTTGCGCT‑CCCTTAATGT‑GCCTCGCCa                                                                                                            <  1:667943/150‑1 (MQ=255)
                                             tCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTG‑TTT‑GGCTTCAGCA‑G‑CGGGTTGCGCT‑CCCTTAATGT‑GCCTCGCCat                                                                                                           <  1:2723402/150‑1 (MQ=255)
                                               aGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTG‑TTT‑GGCTTCAGCA‑G‑CGGGTTGCGCT‑CCCTTAATGT‑GCCTCGCCatat                                                                                                         <  1:2386078/150‑1 (MQ=255)
                                               aGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTG‑TTT‑GGCTTCAGCA‑G‑CGGGTTGCGAT‑CCCTTAATGT‑GCCTCGCCatat                                                                                                         <  1:3244938/150‑1 (MQ=255)
                                                 cggcgATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTG‑TTT‑GGCTTCAGCA‑G‑CGGGTTGCGCT‑CCCTTAATGT‑GCCTCGCCATATaa                                                                                                       <  1:2953409/150‑1 (MQ=255)
                                                   gcgATGCAAAAACGCTTGGCGGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTG‑TTT‑GGCTTCAGCA‑G‑CGGGTTGCGCT‑CCCTTAATGT‑GCCTCGCCATATAAAt                                                                                                     <  1:1953682/150‑1 (MQ=255)
                                                    cgATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGGTTTG‑TTTGGGTTTCAGAACG‑GGGGTTG‑GGT‑CCC‑TGATGT‑GCCCCCCCGTATAAAtt                                                                                                    >  2:3248485/1‑150 (MQ=255)
                                                     gATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTG‑TTT‑TGCTTCCGCA‑G‑CGGGGTTCGCT‑CCCCTTATGT‑GCCTCGCCCTATAAAttt                                                                                                   >  1:3825282/1‑149 (MQ=255)
                                                      aTGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTG‑TTT‑GGCTTCAGCA‑G‑CGGGTTGCGCT‑CCCTTAATGT‑GCCTCGCCATATAAATTGa                                                                                                  <  1:750828/150‑1 (MQ=255)
                                                      aTGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGGTTTT‑TTT‑TGGTTCAGCA‑G‑CGGGGTGCGCT‑CCCCTTATGT‑GCCTCGCCCTATAAAttta                                                                                                  >  1:663036/1‑148 (MQ=255)
                                                       tGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTG‑TTT‑GGCTTCAGCA‑G‑CGGGGTGCGCT‑CCCTTAATGT‑GCCTCGCCCTATAAATTGaa                                                                                                 >  1:1145790/1‑150 (MQ=255)
                                                         cAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTG‑TTT‑GGCTTCAGCA‑G‑CGGGTTGCGCT‑CCCTTAATGT‑GCCTCGCCATATAAATTGAA‑Tg                                                                                              <  1:1580208/150‑1 (MQ=255)
                                                          aaaaaCGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTT‑TTT‑TGCTTCCGCA‑G‑CGGGTTGCCGT‑CCCTTTATTT‑GCCTCGCCCTATAAATTGAA‑Tgg                                                                                             >  1:1386063/1‑150 (MQ=255)
                                                           aaaaCGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTG‑TTT‑GGCTTCAGCA‑G‑CGGGTTGCGCT‑CCCTTAATGT‑GCCTCGCCATATAAATTGAA‑TGGt                                                                                            <  1:2981945/150‑1 (MQ=255)
                                                             aaCGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTG‑TTT‑GGCTTCAGCA‑G‑CGGGTTGCGCT‑CCCTTAATGT‑GCCTCGCCATATAAATTGAA‑TGGTGc                                                                                          <  1:800321/150‑1 (MQ=255)
                                                              aCGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTT‑TTT‑TGCTTTAGCC‑G‑CGGGGTGCGCC‑CCCTTTATGT‑TGCTCCCCCTTTTAATTGTA‑ttggggg                                                                                         >  2:2795410/1‑144 (MQ=255)
                                                                    tGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTG‑TTT‑GGCTTCAGCA‑G‑CGGGTTGCGCT‑CCCTTAATGT‑GCCTCGCCATATAAATTGAA‑TGGTGCAGGGAgc                                                                                   <  1:1875601/150‑1 (MQ=255)
                                                                      ccGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTG‑TTT‑GGCTTCAGCA‑G‑CGGGTTGCGCT‑CCCTTAATGT‑GCCTCGCCATATAAATTGAA‑TGGTGCAGGGAgcgc                                                                                 <  1:11004/150‑1 (MQ=255)
                                                                        gAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTT‑TTT‑TGGTTCCGCC‑G‑CGGGGTGCGCT‑CCCTTTATTT‑TCCTCCCCCTCTAAATTGTA‑TGGTGGAGGGGgggcg‑c                                                                              >  2:1288211/1‑150 (MQ=255)
                                                                             cGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTG‑TTT‑GGCTTCAGCA‑G‑CGGGTTGCGCT‑CCCTTAATGT‑GCCTCGCCATATAAATTGAA‑TGGTGCAGGGAGCGCG‑CAgggg                                                                         <  1:1344243/150‑1 (MQ=255)
                                                                              gATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGGTTTG‑TTT‑TGCTTCTGCA‑G‑CGGGGGGCGGC‑CCCCTTATGT‑GCCTCCCCCTATAAAATTAA‑AGGTGCAGGGGGCGCG‑CCggggg                                                                        >  2:751476/1‑150 (MQ=255)
                                                                               aTGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTG‑TTT‑GGGTTCAGCA‑G‑CGGGGTGGGGG‑CCCTTTATGG‑GGCTCGCCCCATAAAATTCA‑TGGGGCAGGGGGGGCG‑CCgggggg                                                                       >  2:3830419/1‑149 (MQ=255)
                                                                                 gcgcACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTG‑TTT‑GGCTTCAGCA‑G‑CGGGTTGCGCT‑CCCTTAATGT‑GCCTCGCCATATAAATTGAA‑TGGTGCAGGGAGCGCG‑CAGGGggcgg                                                                     <  2:1164140/150‑1 (MQ=255)
                                                                                          cATTCGGCTCCGCCGTCACGATTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTT‑TTT‑TGGTTCAGCA‑G‑GGGGGTGGGCC‑CCCCTTATGT‑GGCCCCCCCTATTAATTGAATTGGGGCGGGGGGGGGC‑CGGGGGGGGGCCaacgcc                                                             >  2:1200677/1‑146 (MQ=255)
                                                                                                 cTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTT‑TTT‑TGGTTTAACC‑A‑CGGGGTGCGCC‑CCCTTTATTT‑GGCTCGCCCTATAAATTTAA‑TGGTGGAAGGAGCGCG‑CCGGGGGGGGCCCATCGCCGCCGccc                                                     >  2:255824/1‑150 (MQ=255)
                                                                                                 cTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGGTTTT‑TTT‑TGGTTCAGCA‑A‑CGGGGTGGGCTCCCCTTAAT‑T‑GTCCCGCCCTATAAAATTAA‑TAGGGGAGGGGGGGCGCCGGGGGGGGGCCAATcccccccccc                                                      >  2:3896708/1‑150 (MQ=255)
                                                                                                  tCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTT‑TTT‑TGGTTCAGCA‑G‑CGGGGTGGGCC‑CCCCTTATGTGGCCCCCCCAATTAAATT‑AA‑TGGGGGAGGGGGGGGG‑CCGGGGGCGGCCCATcccccccccccc                                                    >  2:2048192/1‑150 (MQ=255)
                                                                                                    cgccgTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTG‑TTT‑GGCTTCAGCA‑G‑CGGGTTGCGCT‑CCCTTAATGT‑GCCTCGCCATATAAATTGAA‑TGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCct                                                  <  1:169688/150‑1 (MQ=255)
                                                                                                          cACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTT‑TTT‑TGGTTCCGCA‑G‑CGGGGTGCGCT‑CCCCTAATTT‑GCCTCGCCATATAAATTGAA‑TGGTGCAGGGGGCGCG‑CAGGGGGCGGCCAATCGCCCCCGCCCCCCGCTGT‑c                                           >  2:2416453/1‑150 (MQ=255)
                                                                                                             ggTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTT‑TTT‑TGGTTTAACA‑G‑CGGGGTGCGCT‑CCCCTTATGT‑GCCTCCCCCTATAAATTGAA‑TGGGGCAGGGGGCGCG‑CAGGGGGGGGGCCATCCCCCCCGCCCCCCCCCGT‑cccc                                        >  2:1345556/1‑149 (MQ=255)
                                                                                                             ggTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTG‑TTT‑GGCTTCAGCA‑G‑CGGGTTGCGCT‑CCCTTAATGT‑GCCTCGCCATATAAATTGAA‑TGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGT‑CCCg                                        <  1:2139867/150‑1 (MQ=255)
                                                                                                                 gCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTT‑TTT‑TGGTTTCGCA‑G‑CGGGGTGTGCT‑CCCTTTATGT‑GCCTCCCCCTATAAAATGAA‑TGGTGCGGGGAGGGGG‑CGTGGGGGGGCCGATCGCCCCCGCCCCCCGCCGT‑TCCGGccc                                    >  2:3339046/1‑149 (MQ=255)
                                                                                                                 gCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTG‑TTT‑GGCTTCAGCA‑G‑CGGGTTGCGCT‑CCCTTAATGT‑GCCTCGCCATATAAATTGAA‑TGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGT‑CCCGGCCt                                    <  2:2643860/150‑1 (MQ=255)
                                                                                                                  cAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTT‑TTT‑TGCTTCACCA‑G‑CGGGGTGGGCG‑CCCCTTATTT‑GCCCCGCCCTATAAATTGAA‑TGGGGCAGGGGGCGCG‑CCGGGGGGGGGCCATCGCCCCCGCCCCCCGCTGTCCCCGGCCt                                    >  2:2174573/1‑150 (MQ=255)
                                                                                                                   aGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTG‑TTT‑GGCTTCAGCA‑G‑CGGGTTGCGCT‑CCCTTAATGT‑GCCTCGCCATATAAATTGAA‑TGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGT‑CCCGGCCTTc                                  <  1:969385/150‑1 (MQ=255)
                                                                                                                              gggACTTAGCCTGCGGC‑GGTTTTT‑TTT‑TGCTTTTGCA‑G‑CGGGGTGCGCT‑CCCCTTATGT‑GCCTCCCCCTTTAAATTTAA‑TGGTGCAGGGGGGGGG‑CCGGGGGGGGCCCAACGCCGCCCCCCCCCGCTGT‑TCCCGCCCTCGGGGGACGcgt                       >  2:1520935/1‑148 (MQ=255)
                                                                                                                              gggACTTAGCCTGCGGC‑GGTTTTG‑TTT‑GGCTTCAGCA‑G‑CGGGTTGCGCT‑CCCTTAATGT‑GCCTCGCCATATAAATTGAA‑TGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGT‑CCCGGCCTTCGGGGAACGCtt                       <  1:2389066/150‑1 (MQ=255)
                                                                                                                               ggACTTAGCCTGCGGC‑GGTTTTG‑TTT‑GGCTTCAGCA‑G‑CGGGTTGCGCT‑CCCTTAATGT‑GCCTCGCCATATAAATTGAA‑TGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGT‑CCCGGCCTTCGGGGAACGCTTc                      <  1:12238/150‑1 (MQ=255)
                                                                                                                                     aGCCTGCGGC‑GGTTTTG‑TTT‑TGCTTCAGCA‑G‑CGGGGTTCGCT‑CCCCTAATGT‑GGCCCGCCCTATAAATTGAA‑TTGTGGAGGGAGGGCG‑CCGGGGGCGGCCCATCGCCGCCGCCCCCCGCTGT‑CCCCGCCTTCGGGGGACGCGTCAGCGAt                >  2:18234/1‑150 (MQ=255)
                                                                                                                                     aGCCTGCGGC‑GGTTTTG‑TTT‑GGCTTCAGCA‑G‑CGGGTTGCGCT‑CCCTTAATGT‑GCCTCGCCATATAAATTGAA‑TGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGT‑CCCGGCCTTCGGGGAACGCTTCAGCGAt                <  2:804309/150‑1 (MQ=255)
                                                                                                                                       ccTGCGGC‑GGTTTTG‑TTT‑GGCTTCAGCA‑G‑CGGGTTGCGCT‑CCCTTAATGT‑GCCTCGCCATATAAATTGAA‑TGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGT‑CCCGGCCTTCGGGGAACGCTTCAGCGAttt              <  1:841574/150‑1 (MQ=255)
                                                                                                                                         tGCGGC‑GGTTTTG‑TTT‑GGCTTCAGCA‑G‑CGGGTTGCGCT‑CCCTTAATGT‑GCCTCGCCATATAAATTGAA‑TGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGT‑CCCGGCCTTCGGGGAACGCTTCAGCGATTTTg            <  1:2819925/150‑1 (MQ=255)
                                                                                                                                          gcggc‑gGTTTTG‑TTT‑GGCTTCAGCA‑G‑CGGGTTGCGCT‑CCCTTAATGT‑GCCTCGCCATATAAATTGAA‑TGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGT‑CCCGGCCTTCGGGGAACGCTTCAGCGATTTTGa           <  1:2414531/150‑1 (MQ=255)
                                                                                                                                             gc‑ggTTTTG‑TTT‑GGCTTCAGCA‑G‑CGGGTTGCGCT‑CCCTTAATGT‑GCCTCGCCATATAAATTGAA‑TGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGT‑CCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGc        >  2:21649/1‑150 (MQ=255)
                                                                                                                                              c‑ggTTTTG‑TTT‑GGCTTCAGCA‑G‑CGGGTTGCGCT‑CCCTTAATGT‑GCCTCGCCATATAAATTGAA‑TGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGT‑CCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGcc       <  2:743160/150‑1 (MQ=255)
                                                                                                                                                  ttttG‑TTT‑GGCTTCAGCA‑G‑CGGGTTGCGCT‑CCCTTAATGT‑GCCTCGCCATATAAATTGAA‑TGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGT‑CCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGccacc    >  2:839670/1‑150 (MQ=255)
                                                                                                                                                  ttttG‑TTT‑GGCTTCAGCA‑G‑CGGGTTGCGCT‑CCCTTAATGG‑GCCTCGCCATATAAATTGAA‑TGGGGCAGGGAGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGT‑CCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGccacc    >  2:1120370/1‑150 (MQ=255)
                                                                                                                                                   tttg‑ttt‑gGCTTCAGCA‑G‑CGGGTTGCGCT‑CCCTTAATGT‑GCCTCGCCATATAAATTGAA‑TGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGT‑CCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGccacca   >  2:404881/1‑150 (MQ=255)
                                                                                                                                                   tttg‑ttt‑gGCTTCAGCA‑G‑CGGGTTGCGCT‑CCCTTAATGT‑GCCTCGCCATATAAATTGAA‑TGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTCCTG‑‑CCCCGCCTTCCGGGGACGCTTCAGCGGTTTTGAAGCCACCaa  >  1:2536431/1‑150 (MQ=38)
                                                                                                                                                    ttg‑ttt‑gGCTTCAGCA‑G‑CGGGTTGCGCT‑CCCTTAATGT‑GCCTCGCCATATAAATTGAA‑TGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGT‑CCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGCCACCaa  <  1:1354284/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                            
GGCAGTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTG‑TTT‑GGCTTCAGCA‑G‑CGGGTTGCGCT‑CCCTTAATGT‑GCCTCGCCATATAAATTGAA‑TGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGT‑CCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGCCACCAA  >  AP012306/934905‑935201

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑