Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123061,267,5780TG24.4% 171.5 / 22.1 82D60A (GAC→GCC) ddpBD‑Ala‑D‑Ala transporter subunit
Reads supporting (aligned to +/- strand):  ref base T (27/35);  new base G (20/0);  total (47/35)
Fisher's exact test for biased strand distribution p-value = 2.39e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 133 total aligned reads displayed.

GCAGCAGGGCACCAAAAGCCAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCT‑GGCGAATATTT‑TCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCC  >  AP012306/1267429‑1267721
                                                                                                                                                     |                                                                                                                                                 
gcagcagGGCACCAAAAGCCAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGt                                                                                                                                                   <  2:2142509/150‑1 (MQ=255)
  agcagGGCACCAAAAGCCAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTcc                                                                                                                                                 >  1:483071/1‑150 (MQ=255)
    cagGGCACCAAAAGCCAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAg                                                                                                                                               <  1:1969410/150‑1 (MQ=255)
       ggCACCAAAAGCCAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTcc                                                                                                                                            <  1:2591849/150‑1 (MQ=255)
       ggCACCAAAAGCCAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTcc                                                                                                                                            <  2:3361283/150‑1 (MQ=255)
       ggCACCAAAAGCCAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGGCCAGTcc                                                                                                                                            >  2:788708/1‑150 (MQ=255)
       ggCACCAAAAGCCAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGGCCAGGcc                                                                                                                                            >  2:3154911/1‑150 (MQ=255)
        gCACTAAAAGCCAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTccc                                                                                                                                           <  1:2303529/150‑1 (MQ=255)
         cACCAAAAGCCAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCa                                                                                                                                          <  2:2741929/150‑1 (MQ=255)
          aCCAAAAGCCAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAg                                                                                                                                         <  1:776008/150‑1 (MQ=255)
           ccAAAAGCCAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCCGt                                                                                                                                        >  1:3803099/1‑150 (MQ=255)
           ccAAAAGCCAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGt                                                                                                                                        <  2:1327159/150‑1 (MQ=255)
            cAAAAGCCAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGtt                                                                                                                                       <  2:1325101/150‑1 (MQ=255)
            cAAAAGCCAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGtt                                                                                                                                       <  1:184329/150‑1 (MQ=255)
            cAAAAGCCAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGGCCAGTCCCAGtt                                                                                                                                       >  1:110180/1‑150 (MQ=255)
             aaaaGCCAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTg                                                                                                                                      <  2:2535132/150‑1 (MQ=255)
             aaaaGCCAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACAATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGGTg                                                                                                                                      >  1:2628349/1‑150 (MQ=255)
              aaaGCCAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGc                                                                                                                                     <  2:373200/150‑1 (MQ=255)
                aGCCAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGGCCCGTCCCAGGTGCT‑g                                                                                                                                  >  2:3910060/1‑150 (MQ=255)
                  ccAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCT‑GGc                                                                                                                                <  1:1017423/150‑1 (MQ=255)
                  ccAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGGCCAGTCCCAGTTGCT‑GGc                                                                                                                                >  1:171266/1‑150 (MQ=255)
                      cTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCT‑GGCGAat                                                                                                                            <  1:286068/150‑1 (MQ=255)
                      cTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGGCCCCTCCCAGTTTCT‑GGCGagt                                                                                                                            >  2:2412411/1‑148 (MQ=255)
                        ccAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCT‑GGCGAatat                                                                                                                          >  1:340773/1‑150 (MQ=255)
                          aGCGTCGTCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCT‑GGCGAATAttt                                                                                                                        <  1:3647805/150‑1 (MQ=255)
                            cGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGGCCAGTCCCAGTTGCT‑GGGGGATATTT‑Tc                                                                                                                     >  2:2468531/1‑150 (MQ=255)
                              tCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCT‑GGCGAATATTT‑TCCa                                                                                                                   <  1:1125941/150‑1 (MQ=255)
                              tCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGGCCAGTCCCAGCTGCT‑GGCGCATAttt‑ttct                                                                                                                   >  2:2969303/1‑147 (MQ=255)
                               cGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCT‑GGCGAATATTT‑TCCAc                                                                                                                  <  1:1590019/150‑1 (MQ=255)
                                gCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCT‑GGCGCAAATTT‑TTCACg                                                                                                                 >  2:766343/1‑150 (MQ=255)
                                 ccGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCT‑GGCGAATATTT‑TCCACGa                                                                                                                <  1:1921242/150‑1 (MQ=255)
                                   gggAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCT‑GGCGAATATTT‑TCCACGATa                                                                                                              <  2:608527/150‑1 (MQ=255)
                                   gggAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCT‑GGCGAATATTT‑TCCACGATa                                                                                                              <  1:1300333/150‑1 (MQ=255)
                                    ggAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCT‑GGCGAATATTT‑TCCACGATAg                                                                                                             <  2:3317522/150‑1 (MQ=255)
                                    ggAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCT‑GGCGAATATTT‑TCCACGATAg                                                                                                             <  2:1894229/150‑1 (MQ=255)
                                     gAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCT‑GGCGAATATTT‑TCCACGATAGc                                                                                                            <  1:956959/150‑1 (MQ=255)
                                     gAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCT‑GGCGAATATTT‑TCCACGATAGc                                                                                                            <  1:106017/150‑1 (MQ=255)
                                     gAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCT‑GGCGAATATTT‑TCCACGATAGc                                                                                                            <  2:2293895/150‑1 (MQ=255)
                                      aaaaaTATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCT‑GGCGAATATTT‑TCCACGATAGCa                                                                                                           <  2:501307/150‑1 (MQ=255)
                                       aaaaTATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCT‑GGCGAATATTT‑TCCACGATAGCAt                                                                                                          <  2:2044065/150‑1 (MQ=255)
                                        aaaTATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCCGTCCCAGTTGCT‑GGCGAATATTT‑TCCACGATAGCATc                                                                                                         >  1:3938424/1‑150 (MQ=255)
                                         aaTATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGGCCAGTCCCAGTTGCT‑GGCGAAAATTT‑TTCACGATAGCATCg                                                                                                        >  2:3143177/1‑150 (MQ=255)
                                          atatACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGGCCCGACCCAGTTGGT‑GGCGAAAATTT‑TTCACGATAGCGTccc                                                                                                       >  2:1680629/1‑148 (MQ=255)
                                          atatACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGGCCAGTCCCCGCTGCT‑GGCGAAAATTT‑TTCACCATAGCATctc                                                                                                       >  2:220033/1‑148 (MQ=255)
                                          atatACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGGCCAGTCCCAGTTGCT‑GGGGGAAATTT‑TTCACGATAGCATCGc                                                                                                       >  2:3147240/1‑150 (MQ=255)
                                           tataCGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCT‑GGCGAATATTT‑TCCACGATAGCATCGCt                                                                                                      <  1:1220924/150‑1 (MQ=255)
                                           tataCGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGGCCAGTCCCAGTTGCT‑GGCGAATATTT‑TTCACCATAGCATCGCt                                                                                                      >  1:3987601/1‑150 (MQ=255)
                                            ataCGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCT‑GGCGAATATTT‑TCCACGATAGCATCGCTg                                                                                                     >  1:3577206/1‑150 (MQ=255)
                                            ataCGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCT‑GGCGAATATTT‑TCCACGATAGCATCGCTg                                                                                                     <  1:786871/150‑1 (MQ=255)
                                            ataCGCAACCCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCT‑GGCGAATATTT‑TCCACGATAGCATCGCtt                                                                                                     >  1:3833413/1‑149 (MQ=255)
                                              aCGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCT‑GGCGAATATTT‑TCCACGATAGCATCGCTGgc                                                                                                   <  2:1874388/150‑1 (MQ=255)
                                               cGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGGCCAGTCCCAGTTGCT‑GGCGGATATTT‑TCCACGAAAGCATCGCTGgcg                                                                                                  >  2:1514666/1‑150 (MQ=255)
                                                gCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCT‑GGCGAATATTT‑TCCACGATAACATCGCTGgcgc                                                                                                 >  1:1297240/1‑150 (MQ=255)
                                                gCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGGCCAGTCCCCGTTGCT‑GGCGAAAATTT‑TCCCCCATAACATCGCCGgccc                                                                                                 >  2:1164806/1‑148 (MQ=255)
                                                  aaCTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCT‑GGCGAATATTT‑TTCACGATAGCATCGCTTGCGCgg                                                                                               >  1:2385563/1‑150 (MQ=255)
                                                     tctTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCT‑GGCGAATATTT‑TCCACGATAGCATCGCTGGCGCGGTCa                                                                                            >  1:2478700/1‑150 (MQ=255)
                                                       ttCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCT‑GGCGAATATTT‑TCCACGATAGCATCGCTGGCGCGGTCAcc                                                                                          >  1:2773243/1‑150 (MQ=255)
                                                        tCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCT‑GGCGAATATTT‑TCCACGATAGCATCGCTGGCGCGGTCAccc                                                                                         >  1:1484179/1‑150 (MQ=255)
                                                          cAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGGCCAGTCCCAGTTGCT‑GGCGAATATTT‑TCCACGATAGCATCGCTCGCGCGGGCAccccc                                                                                       >  2:2799656/1‑148 (MQ=255)
                                                             cACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCT‑GGCGAATATTT‑TCCCCGATAGCATCGCTGGCGCGGTCAcccccccg                                                                                    >  1:3217836/1‑148 (MQ=255)
                                                             cACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGGCCAATCCCAGTTGCT‑GGGGGAAATTT‑TTCACGATAGCATCGCTGGCGCGGGCAcccccccg                                                                                    >  2:1411028/1‑148 (MQ=255)
                                                              aCCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCT‑GGCGAATATTT‑TCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGt                                                                                   >  1:562382/1‑150 (MQ=255)
                                                                cGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCCGTTGCT‑GGCGAATATTT‑TCCACGATAGCATCGCTGGCGCGGTCACCCGCCCGTaa                                                                                 >  1:3207754/1‑150 (MQ=255)
                                                                  gACCCCCGGTAGGAATGGTTGTCCCGAGGTCCCCATGAAACGGGTCGCGGAGGTAGGGATAAAACTGTACATACAGTGGCTGCCCCAGCCCCGGTTGCG‑GGCAAATTTTT‑TCCACGATAGCACCGCGGGCGGGGCCACCCGCCAGTAAcc                                                                               <  2:219156/150‑1 (MQ=255)
                                                                   aCGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGGCCAGTCCCAGTTGCT‑GGCGGAAATTT‑TTCACGATAGGATCGCTGGCGCGGTCACCCGCCAGTAACcg                                                                              >  2:211720/1‑150 (MQ=255)
                                                                         ggTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCT‑GGCGAATATTT‑TCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCgg                                                                        >  1:3546768/1‑150 (MQ=255)
                                                                            aCGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGGCCAGTCCCAGTTGCT‑GGCGAAAATTT‑TTCCCGATAGCATCGCTGGCGCGGGCACCCGCCCGTAAACGCCCCGGAta                                                                     >  2:1389725/1‑149 (MQ=255)
                                                                             cGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGGCCAGTCCCAGTTGCT‑GGCGGAAATTT‑TTCACGATAGCCACGCTGGCGCGGGCACCCGCCAGTAAACCCGCCGGCTCa                                                                    >  2:2061400/1‑150 (MQ=255)
                                                                              gAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCT‑GGCGAATATTT‑TCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCAc                                                                   >  1:3667335/1‑150 (MQ=255)
                                                                               aaTGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCT‑GGCGAATATTT‑TCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCCGATCAcc                                                                  >  1:3603716/1‑150 (MQ=255)
                                                                               aaTGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGGCCAGTCCCCGTTGCT‑GGCGAATATTT‑TCCCCGAAAGCATCGCTGGCGCGGTCACCCCCCCGGAACCGCGCCCGGTCAcc                                                                  >  2:585053/1‑150 (MQ=255)
                                                                                 tGGTTGTTCCGGGTTCTCCAAAAAACGGGTCGCGGACGTGGCGTAAACCCTGTACAT‑CAGTGGCGGGCCCGTTTCCCGTTGGC‑GGGGAATTTTTCCCCAAGAGACCGGCGCGGGCGCGGCCCCCCGCCAGTAACCGCGCCGGATCACCAg                                                                <  2:564595/150‑1 (MQ=255)
                                                                                  ggATCTCCCCAGGCCCCCAAAAAACGGGCCGCGGCCGTGGGGAAAAAACTGAACAAACAGTGGCGGGCCCGGCCCCGTTTGCG‑GGCAATTTTTT‑CCCACAATGCCACCGGGGGGGGGGCCCCCCGCCAGTAACCGCGCCGGATCACCAgg                                                               <  2:3004373/150‑1 (MQ=255)
                                                                                    aTGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCT‑GGCGGATATTT‑TTCCCGATAGCATCCCTGGCGCGGGCACCCGCCAGTAACCGCGCCCGGTCACCCGGGa                                                             >  2:1980377/1‑150 (MQ=255)
                                                                                      gccccgaGGCCACAATGAAGGGGGTGGCTGACGTGGCGAAAAATTTGAACATACGGTGGCTGGCCCTG‑CCCAGGTGGTGGGAAAATTTTT‑TCCGCAACGCCGCGGCGGGGGGGGTCCCCCGCCAGTAACCGCGCCGGATCACCAGGGATc                                                           <  2:1572782/144‑1 (MQ=255)
                                                                                           cAGGTCCCCAAGAAACAGGTCGCTGGCGGGGGGAAAAAACTGTACATACGGGGGCTGGCCCAGTCCCGGTTGCG‑GGCAAATTTTT‑CCCACGAAAGCACCGCTGGGGGGGCCCCCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATg                                                      <  2:3007730/150‑1 (MQ=255)
                                                                                           cAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCT‑GGCGAATATTT‑TCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCCGATCACCAGGGATCAGATg                                                      >  1:2452335/1‑150 (MQ=255)
                                                                                            aGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCACTCCCATATACT‑GGCGAAAATTT‑TCCACCAAAACATCGCCCGGGGGGGGCCCCCCCAGCAACCGCCCCGGATCACCAAGGAGCAGAAGc                                                     >  2:2544206/1‑150 (MQ=255)
                                                                                                cACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGGCCAGGCCCAGGTGCT‑GGCGAATATTT‑TTCACGATAGCATCCCCGGCGCGGGCACCCCCCCGGAAACGCGCCGGATCACCAGGGGGCAGATGCgaga                                                 >  2:2651839/1‑150 (MQ=255)
                                                                                                  ccATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGGCCAGTCCCCGTTGCT‑GGGGAATTTTT‑TCCACCAGAGCCTCCCCCGCGCGGTCACCCCCCCGTAAACCCCCCGGCACACCCGGGGTCAGATGCGAGata                                               >  2:2136454/1‑150 (MQ=255)
                                                                                                    aTGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGGCCCCTCCCAGGTGCT‑GGCGGATATTT‑TTCCCGATATCACCTCGGGCGCGGGCACCCCCCCGTAACCGCGCCCGGTCCCCAGGGGTCAGAGGCGTGagaaa                                             >  2:3670898/1‑149 (MQ=255)
                                                                                                      gAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGGGCAGGCCCAGGTGGG‑GGGGAAAATTT‑TCCACGATAGGAACGGTGGGGGGGGGACCCGCCAGGAAACGCGCCGGGGCACCAGGGATCAGATGGGAGAtaaaaa                                           >  2:3595193/1‑150 (MQ=255)
                                                                                                          cAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGGCCAGTCCCAGTTGCT‑GGCGAATATTT‑TCCACGATAGCATCGCTGGCGCGGTCACCCGCCCGTAACCGCGCCGGATCACCAGGGGTCAGATGCGAGATAATAAACGt                                       >  2:2564419/1‑150 (MQ=255)
                                                                                                           gggTCGCTGACGTAGCGGAAAAACTGTACATACGGGGGCTGGCCCAGCCCGAGTGGCT‑GGCGAATATTT‑CCCACGATAGCGTCGCGGGCGCGGCCCCCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTa                                      <  2:3594602/149‑1 (MQ=255)
                                                                                                           aGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGGCCAAGCCCAGCTGCT‑GGCGGATATTT‑TTCACCAAAAAAACGCTAGCGCGGGCACCCGCCACGAACCCCGCCCGATTACCAGGGGTCAGAAGCGGGATaaaaaaagga                                      >  2:3538064/1‑146 (MQ=255)
                                                                                                            ggTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCT‑GGCGAATATTT‑TTCACGATAGCATCGCTGGCGCGGTCACCCGCCCGTAACCGCGCCGGATCACCCGGGATCAGATGCGAGATAATAAACGTaa                                     >  2:2503894/1‑150 (MQ=255)
                                                                                                                  tGACGTAGCGGTAAAACTGTACATACAGTGGCTGGGCCAGTCCCAGTTGCT‑GGCGAATATTT‑TCCACGATAGCATCGCTGGCGCGGGCACCCGCCCGGAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGc                               >  1:1561473/1‑150 (MQ=255)
                                                                                                                    agtgaGCGGTAAAACTATACATACTGGGGCGGTTCCAGCCCCTTTGGGG‑GGAAAATTTTT‑CCCACGATGGCAGCGCTGGCGCGTACCCCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAg                             <  2:1545154/146‑1 (MQ=255)
                                                                                                                    aCGTAGCGGTAAAACTGTACATACAGTGGCTGGGCCATCCCCAGCTGGT‑GGCGAAAATTT‑TTCACGATAGCATCGCCGGCGCGGTCACCCCCCCGTAACCGCGCCCGGACACCAGGGATCCGATGCGAGATAATAAACGAAATCACGccg                             >  2:3278041/1‑148 (MQ=255)
                                                                                                                     cGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCT‑GGCGAATATTT‑TCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGa                            >  1:81779/1‑150 (MQ=255)
                                                                                                                     cGTAGCGGTAAAACTGTACATACAGTGGCTGGGGCAGTCCCAGTTGCT‑GGGGGAAATTT‑TTCACCATAGCATCGCCGGCGCGGGCACCCCCCCGTAACCGCGCCCGGACACCAAGGGGCAGAAGCGGGATAAAAAAAGGAATCACGCaca                            >  2:1923145/1‑148 (MQ=255)
                                                                                                                     cGTAGCGGTAAAACTGTACATACAGTGGCTGGGCCAGTCCCAGGTGCT‑GGCGGAAATTT‑TCCACCATAGCATCGCCTGCGCGGGCACCCCCCAGTAACCGCGCCGGGTCACCAGGGATCAGATGCGAGGTAAAAAAAGGAATCACCCAGa                            >  2:419224/1‑150 (MQ=255)
                                                                                                                        aGCGGAAACACTTAACAAAGAGGGGCTGGCCCGGCCCCGGGTGCG‑GAAAAATTTTT‑TCCAGGATCGCACCGCGGGGGCGGCCCCCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGAcgc                         <  2:478858/150‑1 (MQ=255)
                                                                                                                           ggTAAAACTGTACAGACAGTGGCTGGTCCAGTCCCAGTTGCT‑GGCGAATATTT‑TCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGAcgcccg                      <  1:3364647/150‑1 (MQ=255)
                                                                                                                            gTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCT‑GGCGAATATTT‑TCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGAcgcccgc                     >  1:3484246/1‑150 (MQ=255)
                                                                                                                             tAAAACTGTACATACAGTGGCTGGGCCAGTCCCACGTGGT‑GGAGAAAAATT‑TTCACCAGAGCAACGCCCGCGCCGGCACCCCCCAGGAAACGCCCCGGGCCACCAAGGATCAAATGCGAGGGAAAAAAAATAAACACGCAGAcgcccccc                    >  2:146338/1‑150 (MQ=255)
                                                                                                                                  cTGTACATACAGTGGCTGGGCCAGGCCCAGTTGCT‑GGCGAATATTT‑TTCACGATAGGATCGCTGGGGCGGGCACCCGCCAGGAACCCCGCCGGAGCACCCGGGAGCAGATGCGGGATAATAAACGGAATCACGCAAACCCCCCccacccc               >  2:1670168/1‑148 (MQ=255)
                                                                                                                                      acatacaGTGGCTGGTCCAGTCCCAGTTGCT‑GGCGAATATTT‑TCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGc           >  1:3288009/1‑150 (MQ=255)
                                                                                                                                       catacaGTGGCTGGTCCAGTCCCAGTTGCT‑GGCGAAAATTT‑TCCACCATAGCATCGCTGGCGCGGTCACCCGCCCGTAACCCCGCCGGAACACCAAGGGTCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCCCCACCCCCAGCa          >  2:184136/1‑150 (MQ=255)
                                                                                                                                           caGTGGCTGGGCCCGTCCCAGTTTCT‑GGCGAAAAATT‑TTCACGATAGCGACTCTGGCGCGGGTCCCCGCCAGTAACCGCGCCGGATCACCAGGGGTCAGAAGCGAGATAATAAACCTAATCACGCAGACGCCCGCCACCCCCAGCGCCaa      >  2:743219/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
GCAGCAGGGCACCAAAAGCCAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCT‑GGCGAATATTT‑TCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCC  >  AP012306/1267429‑1267721

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑