Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123061,421,1470TG26.5% 439.6 / 16.4 224T79P (ACC→CCC) ydhBpredicted DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (79/85);  new base G (59/0);  total (139/85)
Fisher's exact test for biased strand distribution p-value = 1.14e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 274 total aligned reads displayed.

TTCCTGAAAGACAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACA‑GAGCGCCCTTCTTTGAG‑AAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCGGCACCGCCAGCCACTCTTCCAGCTGACGCACGGTATAGCTGACCGCAGAAGGAA  >  AP012306/1420998‑1421296
                                                                                                                                                     |                                                                                                                                                       
ttCCTGAAAGACAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGt                                                                                                                                                         >  2:2699929/1‑150 (MQ=255)
ttCCTGAAAGACAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGt                                                                                                                                                         <  2:1687959/150‑1 (MQ=255)
   cTGAAAGACAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGAt                                                                                                                                                      <  2:1424267/150‑1 (MQ=255)
    tGAAAGACAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGGTc                                                                                                                                                     >  2:1068622/1‑150 (MQ=255)
    tGAAAGACAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATc                                                                                                                                                     <  2:1900417/150‑1 (MQ=255)
     gAAAGACAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCt                                                                                                                                                    <  2:862315/150‑1 (MQ=255)
      aaaGACAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTg                                                                                                                                                   >  1:2208721/1‑150 (MQ=255)
       aaGACAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGGTCtac                                                                                                                                                  >  1:2930797/1‑148 (MQ=255)
        aGACAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTCCGATAGCTAACTGACTGCGCCATCCGTTCGCTATATGCTGACATTGCTGGCGGAGGTTCTGct                                                                                                                                                 >  2:1506614/1‑149 (MQ=255)
          aCAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCAtt                                                                                                                                               <  2:3529831/150‑1 (MQ=255)
          aCAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCAtt                                                                                                                                               <  1:2148419/150‑1 (MQ=255)
          aCAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATTTGCAtt                                                                                                                                               >  1:1284019/1‑150 (MQ=255)
           cAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCAttt                                                                                                                                              <  1:3721146/150‑1 (MQ=255)
            aagaagTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCAtttt                                                                                                                                             >  1:614700/1‑150 (MQ=255)
            aagaagTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCAtttt                                                                                                                                             <  1:3092968/150‑1 (MQ=255)
             agaagTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCAttttt                                                                                                                                            <  2:234411/150‑1 (MQ=255)
             agaagTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCAttttt                                                                                                                                            >  2:1629520/1‑150 (MQ=255)
               aagTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCAttttttt                                                                                                                                          >  1:2536173/1‑150 (MQ=255)
                agTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCAtttttttt                                                                                                                                         >  2:2214496/1‑149 (MQ=255)
                agTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCAtttttttt                                                                                                                                         >  1:2523568/1‑149 (MQ=255)
                     gACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGacaac                                                                                                                                    <  2:3140900/150‑1 (MQ=255)
                     gACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGGTCTGCCTTTTTTTTacaac                                                                                                                                    >  1:2029665/1‑150 (MQ=255)
                      aCATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCATTTTTTTTacaaca                                                                                                                                   >  2:1832677/1‑150 (MQ=255)
                       catcatCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACa‑g                                                                                                                                 <  2:1995226/150‑1 (MQ=255)
                        atcatcAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCCTTTTTTTGACAACa‑ga                                                                                                                                >  2:1487255/1‑150 (MQ=255)
                         tcatcaAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTTACAACa‑gag                                                                                                                               >  2:3866850/1‑150 (MQ=255)
                            tcaAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACA‑GAgcgc                                                                                                                            <  2:721513/150‑1 (MQ=255)
                             caAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTTACAACA‑GAGCGcc                                                                                                                           >  2:2442801/1‑150 (MQ=255)
                             caAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTTACAACA‑GAGCGcc                                                                                                                           >  1:556737/1‑150 (MQ=255)
                             caAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACA‑GAGCGcc                                                                                                                           >  1:705915/1‑150 (MQ=255)
                                 aTGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGTATTTTTTTGACAACA‑GAGCGCCcttc                                                                                                                       <  2:942815/150‑1 (MQ=255)
                                 aTGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGGTCTGCATTTTTTTTACAACA‑GAGCGCCcctc                                                                                                                       >  2:3887597/1‑150 (MQ=255)
                                  tGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGGTCTGGCTTTTTTTTACAAAA‑GAGCGCCcctct                                                                                                                      >  2:843329/1‑150 (MQ=255)
                                    gCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCATTTTTTTGACAACA‑GAGCGCCCTTCttt                                                                                                                    >  1:2439545/1‑150 (MQ=255)
                                     cGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACA‑GAGCGCCCTTCTTTg                                                                                                                   <  1:1518957/150‑1 (MQ=255)
                                      gATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACA‑GAGCGCCCTTCTTTga                                                                                                                  <  1:1031686/150‑1 (MQ=255)
                                      gATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACA‑GAGCGCCCTTCTTTga                                                                                                                  <  1:954892/150‑1 (MQ=255)
                                      gATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCATTTTTTTTACAACA‑GAGCGCCCTTCTTTga                                                                                                                  >  2:3157274/1‑150 (MQ=255)
                                       aTAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGCGATCTGCATTTTTTTTACAACA‑GAACGCCCCTCTTTtag                                                                                                                 >  1:3378254/1‑150 (MQ=255)
                                         aaaaaTCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTTACAACA‑GAGCGCCCTTCTTTTAG‑aa                                                                                                              >  1:3788396/1‑150 (MQ=255)
                                         aaaaaTCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACA‑GAGCGCCCTTCTTTGAG‑aa                                                                                                              <  2:1457155/150‑1 (MQ=255)
                                         aaaaaTCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCATTTTTTTGACAACA‑GAGCGCCCTTCTTTGAG‑aa                                                                                                              >  1:361397/1‑150 (MQ=255)
                                           aaaTCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACA‑GAGCGCCCTTCTTTGAG‑AAAc                                                                                                            >  1:3297655/1‑150 (MQ=255)
                                               cAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGGTCCGCATTTTTTTTGCAAAA‑CAGCGCCCCTCTTTGAG‑GAACCACg                                                                                                        >  2:2936529/1‑150 (MQ=255)
                                               cAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCATTTTTTTGACAACA‑GAGCGCCCTTCTTTGAG‑AAACCACg                                                                                                        >  1:3440102/1‑150 (MQ=255)
                                                aaCGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCATTTTTTTTACAACA‑GAGCGCCCCTCTTTGAG‑AAACCACGc                                                                                                       >  1:1316066/1‑150 (MQ=255)
                                                aaCGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCATTTTTTTGACAACA‑GAGCGCCCTTCTTTGAG‑AAACCACGc                                                                                                       >  1:2391483/1‑150 (MQ=255)
                                                  cGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGGTCTGCATTTTTTTGACAACA‑GAGCGCCCTTCTTTGAG‑AAACCACGCTc                                                                                                     >  1:3579721/1‑150 (MQ=255)
                                                   gATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTTACAACA‑GAGCGCCCTTCTTTGAG‑AAACCACGCTcc                                                                                                    >  1:3742365/1‑150 (MQ=255)
                                                    atcatcTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGGTCTGCATTTTTTTTGCAACA‑GAGCGCCCTTCTTTGAG‑AAACCACGCTccc                                                                                                   >  2:1144418/1‑149 (MQ=255)
                                                    atcatcTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACA‑GAGCGCCCTTCTTTGAG‑AAACCACGCTCca                                                                                                   >  1:3849966/1‑150 (MQ=255)
                                                    atcatcTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACA‑GAGCGCCCTTCTTTGAG‑AAACCACGCTCca                                                                                                   >  1:2692295/1‑150 (MQ=255)
                                                     tcatcTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTTTCAACA‑GAGCGCCCTTCTTTTTG‑AAAACACCCTCcag                                                                                                  >  2:600430/1‑150 (MQ=255)
                                                     tcatcTGCCATGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGCACTGCATTTTTTTTACAACA‑GAGCGCCCTTCTTTGAGTAAAGCA‑GCTCcag                                                                                                  >  2:2643688/1‑150 (MQ=255)
                                                       atcTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTTACAACA‑GAGCGCCCTTCTTTGAG‑AAACCACGCTCcagcc                                                                                                >  1:3969579/1‑149 (MQ=255)
                                                       atcTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACA‑GAGCGCCCTTCTTTGAG‑AAACCACGCTCcagca                                                                                                <  2:1577062/150‑1 (MQ=255)
                                                        tcTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTTACAACA‑AAGCGCCCCTCTTTGTG‑AAAACACGCTCcagcag                                                                                               >  1:1906142/1‑150 (MQ=255)
                                                            ccGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGCTTTGCATTTTTTTTACAACC‑GAGCGCCCTTCTTTGAG‑AAACCACGCTCCCGCAGCGGt                                                                                           >  2:2639207/1‑150 (MQ=255)
                                                              gtgtACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACA‑GAGCGCCCTTCTTTGAG‑AAACCACGCTCCAGCAGCGGTCa                                                                                         <  1:2872881/150‑1 (MQ=255)
                                                              gtgtACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCATTTTTTTTACAACA‑GAGCGCCCTTCTTTGAG‑AAACCACGCTCCCGCAGCGGGCa                                                                                         >  2:3981076/1‑150 (MQ=255)
                                                                    gTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCTTTTTTTTGTCAACA‑GAGCGCCCCTCTTTGAG‑AAACCACGCTCCAGCAGCGGTCAGCTCca                                                                                   >  2:3474975/1‑150 (MQ=255)
                                                                    gTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACA‑GAGCGCCCTTCTTTGAG‑AAACCACGCTCCAGCAGCGGTCAGCTCca                                                                                   <  2:2809075/150‑1 (MQ=255)
                                                                    gTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCCTTTTTTTTACAACA‑GAGCGCCCTTCTTTGAG‑AAAACACGCTCCAGCAGCGGTCAGCTCca                                                                                   >  2:633449/1‑150 (MQ=255)
                                                                       cTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACC‑AAGCGCCCTTCTTTGTG‑AAACCCCGCTCCAGCAGCGGTTAGCTCCCCAt                                                                                >  1:478292/1‑150 (MQ=255)
                                                                       cTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCATTTTTTTTACAACA‑AAGCGCCCCTCTTTTAG‑AAACCACGCCCCAGCAGCGGGCAGCTCCACAt                                                                                >  2:2844806/1‑150 (MQ=255)
                                                                        tGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGGTCTGCATTTTTTTGACAACA‑GAGCGCCCTTCTTTGAG‑AAACCACGCTCCAGCAGCGGGCAGCTCCACATc                                                                               >  2:3682789/1‑150 (MQ=255)
                                                                         ggCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACA‑GAGCGCCCTTCTTTGAG‑AAACCACGCTCCAGCAGCGGTCAGCTCCACATCa                                                                              >  1:1460533/1‑150 (MQ=255)
                                                                            cTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACA‑GAGCGCCCTTCTTTGAG‑AAACCACGCTCCAGCAGCGGTCAGCTCCCCATCCCgg                                                                           >  2:81588/1‑150 (MQ=255)
                                                                             tGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACA‑GAGCGCCCTTCTTTGAG‑AAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGt                                                                          <  1:1137671/150‑1 (MQ=255)
                                                                              gACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACA‑GAGCGCCCTTCTTTGAG‑AAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTg                                                                         >  1:3608190/1‑150 (MQ=255)
                                                                               aCAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACA‑GAGCGCCCTTCTTTGAG‑AAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGc                                                                        <  1:115572/150‑1 (MQ=255)
                                                                                 aaTATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCATTTTTTTTACAAAA‑AAGCGCCCTTCTTTTAG‑AAACCCCGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCcg                                                                      >  2:2960741/1‑150 (MQ=255)
                                                                                  atatTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACA‑GAGCGCCCTTCTTTGAG‑AAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCcgt                                                                     >  1:2645726/1‑150 (MQ=255)
                                                                                     ttatCCACTGCGGTAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCATTTTTTTTGCAACA‑AAGCGCCCCTCTTTTAG‑AAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCcgtcgt                                                                  >  2:2871816/1‑150 (MQ=255)
                                                                                      tatCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCATTTTTTTGACAACA‑GAGCGCCCTTCTTTTAG‑AAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGtt                                                                 >  1:349660/1‑150 (MQ=255)
                                                                                        tCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCATTTTTTTGACAACA‑GAGCGCCCTTCTTTGAG‑AAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCa                                                               >  1:3222698/1‑150 (MQ=255)
                                                                                         ccACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACA‑GAGCGCCCTTCTTTGAG‑AAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCaa                                                              <  2:3113927/150‑1 (MQ=255)
                                                                                               cGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGGTTTGCTTTTTTTTGTAAACC‑AAGCGCCCCTCTTTGAG‑AAAACCCCCTCCAGCAGGCGTCAGCTCCACATTACTGTGTCCTCGATCCAaaaggg                                                        >  2:3744786/1‑148 (MQ=255)
                                                                                                  tAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGAATTCTGCATTTTTTTAGCAAAAGGATCGCCC‑TCTTTCAG‑AAAACACGCTTCAGCCTCGGTGCGCTCCCCCTCACCTTGCCCTCGTTCCAGGAGCCGCa                                                     >  2:3351581/1‑150 (MQ=255)
                                                                                                    gCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACA‑GAGCGCCCTTCTTTGAG‑AAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCGGCAcc                                                   >  1:3782440/1‑150 (MQ=255)
                                                                                                    gCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACA‑GAGCGCCCTTCTTTGAG‑AAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCGGCAcc                                                   <  2:1257206/150‑1 (MQ=255)
                                                                                                    gCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCATTTTTTTGACAACA‑GAGCGCCCTTCTTTGAG‑AAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCGGCAcc                                                   >  2:2433277/1‑150 (MQ=255)
                                                                                                     cTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACA‑GAGCGCCCTTCTTTGAG‑AAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCGGCACCg                                                  <  1:305448/150‑1 (MQ=255)
                                                                                                     cTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACA‑AAGCGCCCTTCTTTGAG‑AAACCACGCCCCAGCAGCGGTCAGCTCCACATCCCCGTGCCGTCGTTCAAAAAACGGCACCg                                                  >  2:1777874/1‑150 (MQ=255)
                                                                                                      tAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACA‑GAGCGCCCTTCTTTGAG‑AAACCACGCTCCAGCAGCGGTCAGCTCCACATCACCGTGCCGTCGTTCAAAAAGCGGCACCgc                                                 >  2:2745896/1‑150 (MQ=255)
                                                                                                       aaCTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGGTCTGCATTTTTTTGACAACA‑GAGCGCCCTTCTTTGAG‑AAACCACGCTCCAGCAGCGGTCAGCTCCACATCACCGTGCCGTCGTTCAAAGAGCGGCACcccc                                                >  2:67313/1‑150 (MQ=255)
                                                                                                        aCTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATATGCGTTTTTTTGACAACA‑AAGGGCCCCTCCTTTCG‑GAACCACGCTCCAGCACCCGGCCACTCCACATCACCGTGCCGCCGTTCAAAGAGCGGCACCgccc                                               >  2:2564428/1‑149 (MQ=255)
                                                                                                          ggcccGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGGGGGTGATCTGCTTTTTTTTGAAAACA‑GAGCGCCTTTCTTTGAG‑AACCCACGCTCCAGCGGCGGTCACCCCCACATCACGGTGCCGTCGTTAAAAGAGCGGCACCgccagc                                             <  2:292289/147‑1 (MQ=255)
                                                                                                                cgcCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGGTCTGCATTTTTTTTGCAACA‑GAGCGCCCCTCTTTGAG‑AAACCACGCTCCAGCAGCGGGGAGCTCCACATCACCGTGCCGGCGTTCAAAGAACGGCCCCGCCCGCCAccct                                       >  2:2284346/1‑150 (MQ=255)
                                                                                                                cgcCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGGTCTGCATTTTTTTGGCAACA‑AAGCGCCCTTCTTTTAG‑AAACCACGCCCCAGCAGCGGTCAGCTCCACATCACGGGGCCGTCGTTCAAAGAGCGGCACCGCCCGCCCctct                                       >  2:1548490/1‑150 (MQ=255)
                                                                                                                 gcCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATTTGCATTTTTTTTACAAAA‑CAGCGCCCTTCTTTGAG‑AAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGGGCCGTCGTTCAAAGAGCGGCACCGCCAGCCACTCtt                                      >  2:2479210/1‑150 (MQ=255)
                                                                                                                    aGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATGTGGATTTTTTTGGCAACA‑AAGCGCCCCTCTTTGAG‑AAACCCCGCTCCAGCAACGGCCAGCCCCTCAACCCCGGGCCGTCATTCAAACAGCGGCCCCGCCCGCCTCCCTTccc                                   >  2:78693/1‑149 (MQ=255)
                                                                                                                     gCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCATTTTTTTGGCAACA‑GAGCGCCCCTCTTTGAG‑AAACCACGCTCCCGCGACGGTCAGCGCCACATCAACGTGCCGTCGCCCCAAGAGCGGCACCCCCAGCCACTCCTCCAg                                  >  2:343147/1‑150 (MQ=255)
                                                                                                                              tATCTGCTGACATTGCTGGCGGGGGATTTGCATTTTTTTGACAACA‑CAGCGCCCTTCTTTGAG‑AAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCGGCACCCCCAGCCCCTCTTCCAGCTGACGCAc                         >  1:3169044/1‑150 (MQ=255)
                                                                                                                                 ctgctgACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACA‑GAGCGCCCTTCTTTGAG‑AAACCACGCTCCAGCAGCGGTCAGCTCCACATCACCGTGCCGTCGTTCAAAGAGCGGCACCGCCAGCCACTCTTCCAGCTGACGCACGGt                      >  2:2163780/1‑150 (MQ=255)
                                                                                                                                      gACATTGCTGGCGGGGGATCTGCATTTTTTTTACAACA‑AAGCGCCCCTCTTTGAG‑AAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCGGCACCGCCAGCCACTCTTCCAGCTGACGCCCGGTATAGc                 >  2:1844575/1‑150 (MQ=255)
                                                                                                                                         aTTGCTGGCGGGTGATCTGCATTTTTTTTACAACA‑GAGCGCCCTTCTTTTAG‑AAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCGGCACCGCCAGCCACTCTTCCAGCTGACGCACGGTATAGCTGa              >  1:698476/1‑150 (MQ=255)
                                                                                                                                                gCGGGTGATCTGCATTTTTTTGACAACA‑GAGCGCCCTTCTTTGAG‑AAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCGGCACCGCCAGCCACTCTTCCAGCTGACGCACGGTATAGCTGACCGCAGa       >  1:1773438/1‑150 (MQ=255)
                                                                                                                                                    gTGATCTGCATTTTTTTGACAACA‑GAGCGCCCTTCTTTGAG‑AAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCGGCACCGCCAGCCACTCTTCCAGCTGACGCACGGTATAGCTGACCGCAGAAGGa   >  1:84124/1‑150 (MQ=255)
                                                                                                                                                     tGATCTGCATTTTTTTGACAACA‑GAGCGCCCTTCTTTGAG‑AAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCGGCACCGCCAGCCACTCTTCCAGCTGACGCACGGTATAGCTGACCGCAGAAGGaa  >  1:2181850/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                       
TTCCTGAAAGACAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACA‑GAGCGCCCTTCTTTGAG‑AAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCGGCACCGCCAGCCACTCTTCCAGCTGACGCACGGTATAGCTGACCGCAGAAGGAA  >  AP012306/1420998‑1421296

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑