Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123062,120,5050AC23.3% 328.3 / 11.9 134G42G (GGT→GGG) eutQconserved protein
Reads supporting (aligned to +/- strand):  ref base A (74/28);  new base C (0/31);  total (74/60)
Fisher's exact test for biased strand distribution p-value = 2.94e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 201 total aligned reads displayed.

TGTGCGACCAGGCTTTCGGTAAACTGGCCTTCCGGCAGCTGGGCAATGATGGTTTCGCGGATGCGCTGGCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACG‑CCGTTACCGGGATCGATTCATCACATTCGGTGATGGT‑AAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAGTTTTTTCACCTGCTTCCTCCTGTTTGGTGAGAG  >  AP012306/2120356‑2120654
                                                                                                                                                       |                                                                                                                                                     
tgtgCGACCAGGCTTTCGGTAAACTGGCCTTCCGGCAGCTGGGCAATGATGGTTTCGCGGATGCGCTGGCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACG‑CCGTTACCGGGATCGATTCATCACATTCGGTGATGGT‑aaaa                                                                                                                                                       <  1:3364871/150‑1 (MQ=255)
tgtgCGACCAGGCTTTCGGTAAACTGGCCTTCCGGCAGCTGGGCAATGATGGTTTCGCGGATGCGCTGGCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACG‑CCGTTACCGGGATCGATTCATCACATTCGGTGATGGT‑aaaa                                                                                                                                                       >  1:3648343/1‑150 (MQ=255)
tgtgCGACCAGGCTTTCGGTAAACTGGCCTTCCGGCAGCTGGGCAATGATGGTTTCGCGGATGCGCTGGCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACG‑CCCTTACCCGGGTCGATTCATCACATTCGGTGATGGT‑aaaa                                                                                                                                                       >  2:1890834/1‑150 (MQ=255)
   gCGACCAGGCTTTCGGTAAACTGGCCTTCCGGCAGCTGGGCAATGATGGTTTCGCGGATGCGCTGGCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACG‑CCGTTACCGGGATCGATTCATCACATTCGGTGATGGT‑AAAAccc                                                                                                                                                    <  1:907057/150‑1 (MQ=255)
     gACCAGGCTTTCGGTAAACTGGCCTTCCGGCAGCTGGGCAATGATGGTTTCGCGGATGCGCTGGCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGGCCGGGCGCCCGTTGCC‑GGATCGATTCCTTCCAATCGGGGGTGGTGAAAAcccg                                                                                                                                                   >  2:1836055/1‑149 (MQ=255)
     gACCAGGCTTTCGGTAAACTGGCCTTCCGGCAGCTGGGCAATGATGGTTTCGCGGATGCGCTGGCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGGCG‑CCGTTACCGGGGTCGCTTCATCACATTCGGGGATGGT‑AAAACCCAg                                                                                                                                                  >  2:2486215/1‑150 (MQ=255)
     gACCAGGCTTTCGGTAAACTGGCCTTCCGGCAGCTGGGCAATGATGGTTTCGCGGATGCGCTGGCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACG‑CCGTTTCCCGGATCGGTTCATTACATTCGGTGATGGT‑AAAAACCAg                                                                                                                                                  >  1:1960598/1‑150 (MQ=255)
     gACCAGGCTTTCGGTAAACTGGCCTTCCGGCAGCTGGGCAATGATGGTTTCGCGGATGCGCTGGCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACG‑CCGTTACCCGGGTCGATTCATCACATTCGGTGATGGT‑AAAACCCAg                                                                                                                                                  >  2:3147548/1‑150 (MQ=255)
        cAGGCTTTCGGTAAACTGGCCTTCCGGCAGCTGGGCAATGATGGTTTCGCGGATGCGCTGGCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACG‑CCGTTACCGGGATCGATTCATCACATTCGGTGATGGT‑AAAACCCAGTAg                                                                                                                                               >  1:158315/1‑150 (MQ=255)
          ggCTTTCGGTAAACTGGCCTTCCGGCAGCTGGGCAATGATGGTTTCGCGGATGCGCTGGCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGGCG‑CCGTTACCGGGATCGATTCATCACATTCGGTGATGGT‑AAAACCCCGTAGAt                                                                                                                                             >  2:1587240/1‑150 (MQ=255)
          ggCTTTCGGTAAACTGGCCTTCCGGCAGCTGGGCAATGATGGTTTCGCGGATGCGCTGGCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACG‑CCGTTACCGGGATCGATTCATCACATTCGGTGATGGT‑AAAACCCAGTAGAt                                                                                                                                             >  2:3738878/1‑150 (MQ=255)
          ggCTTTCGGTAAACTGGCCTTCCGGCAGCTGGGCAATGATGGTTTCGCGGATGCGCTGGCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACG‑CCGTTACCGGGATCGATTCATCACATTCGGTGATGGT‑AAAACCCAGTAGAt                                                                                                                                             >  2:775871/1‑150 (MQ=255)
             tttCGGTAAACTGGCCTTCCGGCAGCTGGGCAATGATGGTTTCGCGGATGCGCTGGCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACG‑CCGTTACCGGGATCGATTCATCACATTCGGTGATGGT‑AAAACCCAGTAGATCCg                                                                                                                                          <  2:331456/150‑1 (MQ=255)
             tttCGGTAAACTGGCCTTCCGGCAGCTGGGCAATGATGGTTTCGCGGATGCGCTGGCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACG‑CCGTTACCGGGATCGATTCATCACATTCGGTGATGGT‑AAAACCCAGTAGATCCg                                                                                                                                          <  1:2278400/150‑1 (MQ=255)
              ttCGGTAAACTGGCCTTCCGGCAGCTGGGCAATGATGGTTTCGCGGATGCGCTGGCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGGCG‑CCGTTACCCGGGTCGATTCATCACATTTGGTGATGGT‑AAAACCCCGTAGATCCGc                                                                                                                                         >  2:3933704/1‑150 (MQ=255)
                 ggTAAACTGGCCTTCCGGCAGCTGGGCAATGATGGTTTCGCGGATGCGCTGGCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCCCGGACG‑CCGTTACCGGGATCGATTCATCACATTCGGTGGTGGG‑AAAACCCAGTAGATCCGcccc                                                                                                                                      >  2:914219/1‑148 (MQ=255)
                  gTAAACTGGCCTTCCGGCAGCTGGGCAATGATGGTTTCGCGGATGCGCTGGCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACG‑CCGTTACCGGGATCGATTCATCACATTCGGTGATGGT‑AAAACCCAGTAGATCCGCCACt                                                                                                                                     >  1:2093165/1‑150 (MQ=255)
                   tAAACTGGCCTTCCGGCAGCTGGGCAATGATGGTTTCGCGGATGCGCTGGCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCCCGGACG‑CCCTTACCGGGATCGATTCATCACATTCGGTGATGGT‑AAAACCCAGTAGATCCGCCACtt                                                                                                                                    >  2:1126536/1‑150 (MQ=255)
                    aaaCTGGCCTTCCGGCAGCTGGGCAATGATGGTTTCGCGGATGCGCTGGCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACG‑CCGTTACCGGGATCGATTCATCACATTCGGTGATGGT‑AAAACCCAGTAGATCCGCCACTTc                                                                                                                                   >  1:2188881/1‑150 (MQ=255)
                     aaCTGGCCTTCCGGCAGCTGGGCAATGATGGTTTCGCGGATGCGCTGGCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACG‑CCGTTCCCGGGATCGATTCATCACATTCGGTGATGGT‑AAAACCCAGTAGATCCGCCACTTCa                                                                                                                                  <  1:3419434/150‑1 (MQ=255)
                     aaCTGGCCTTCCGGCAGCTGGGCAATGATGGTTTCGCGGATGCGCTGGCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACG‑CCGTTACCGGGGTCGATTCATCACATTCGGTGATGGT‑AAAACCCAGTAGATCCGCCACTTCa                                                                                                                                  >  1:170748/1‑150 (MQ=255)
                       cTGGCTTTCCGGCGGCTGGGCAATGATGGTTTCGGGGATGCGCTGGTTTTCGGTTTTGTCGGCTGGCACGCGGGCGGGCACGGACG‑CCGTTCCCGGGATCGATTCATCACATTCGGTGATGGT‑AAACCCCAGTAGATCCGCCACTTCACg                                                                                                                                <  2:1029676/150‑1 (MQ=255)
                           ccTTCCGGCAGCTGGGCAATGATGGTTTCGCGGATGCGCTGGCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACG‑CCGTTACCGGGATCGATTCATCACATTCGGTGATGGT‑AAAACCCAGTAGATCCGCCACTTCACGGGCt                                                                                                                            <  1:3318755/150‑1 (MQ=255)
                                 ggCAGCTGGGCAATGATGGTTTCGCGGATGCGCTGGCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACG‑CCGTTACCGGGATCGATTCATCACATTCGGTGATGGT‑AAAACCCAGTAGATCCGCCACTTCACGGGCTTCCggg                                                                                                                      >  2:2056308/1‑150 (MQ=255)
                                    aGCTGGGCAATGATGGTTTCGCGGATGCGCTGGCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACG‑CCGTTACCGGGATCGATTCATCACATTCGGTGATGGT‑AAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGta                                                                                                                   >  1:3853418/1‑150 (MQ=255)
                                            aaTGATGGTTTCGCGGATGCGCTGGCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACG‑CCGTTACCGGGATCGATTCATCACATTCGGTGATGGT‑AAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCt                                                                                                           >  1:481703/1‑150 (MQ=255)
                                             atgatgGTTTCGCGGATGCGCTGGCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACG‑CCGTTACCGGGATCGATTCATCACATTCGGTGATGGT‑AAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGGAATAATGCTg                                                                                                          >  1:200901/1‑150 (MQ=255)
                                               gatgGTTTCGCGGATGCGCTGGCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACG‑CCGTTACCGGGATCGATTCATCACATTCGGTGATGGT‑AAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGGAATAATGCTGgc                                                                                                        >  1:525339/1‑150 (MQ=255)
                                                  ggTTTCGCGGATGCGCTGGCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACG‑CCGTTACCGGGATCGATTCATCACATTCGGTGATGGT‑AAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGgcgcg                                                                                                     >  1:3788301/1‑150 (MQ=255)
                                                  ggTTTCGCGGATGCGCTGGCTTTCGGTTTTGTCGGCTGGCACGCGGGCGGGCACGGACG‑CCGTTACCGGGATCGATTCATCACATTCGGTGATGGT‑AAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGgcgcg                                                                                                     <  1:3457189/150‑1 (MQ=255)
                                                     tccgcgGATGCGCTGGCTTGCGTTTTGGTGGGCGGGCACGCGGGCGGGCAGGGACG‑CCGTTACCGGGATCGTTTCATCACATTGGGTGATGGA‑AAACCCCGGTAGTTCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAg                                                                                                  <  2:3975613/148‑1 (MQ=255)
                                                        gcgGATGCGCTGGCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACG‑CCGTTACCGGGATCGATTCATCACATTCGGTGATGGT‑AAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGGAATAATGCTGGCGCGCAGAAc                                                                                               >  2:1286019/1‑150 (MQ=255)
                                                         gggATGGGTTGGTTTTCGTTTTTGGCGGTTGGCACGGTGGGGGGCAGGGACG‑CGGTTCCGGGGATCAATTCATCACATTGGGTGATGGA‑AAACCCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAAcc                                                                                              <  2:1943451/149‑1 (MQ=255)
                                                         cgGATGCGCTGGCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACG‑CCGTTACCGGGATCGATTCATCACATTCGGTGATGGT‑AAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAAcc                                                                                              >  1:302353/1‑150 (MQ=255)
                                                         cgGATGCGCTGGCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACG‑CCGTTACCGGGATCGATTCATCACATTCGGTGATGGT‑AAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAAcc                                                                                              >  1:3585752/1‑150 (MQ=255)
                                                          ggATGCGCTGGCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACG‑CCGTTACCGGGATCGATTCATCACATTCGGTGATGGT‑AAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGGAATAATGCTGGCGCGCAGAACCa                                                                                             >  1:1537402/1‑150 (MQ=255)
                                                              gcgcTGGCTTTCGGTTTTGTCGGCGGGCCCGCTGGCGGGCACGGACG‑CCGTTACCGGGATCGATTCATCACATTGGGTGATGGT‑AAACCCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGa                                                                                         <  2:667117/150‑1 (MQ=255)
                                                                gcTGGCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACG‑CCGTTACCGGGGTCGATTCATCACATTCGGTGATGGT‑AAAAACCAGTAGATCCGCCACTTCACGGGCTTCCGGGGGAATAATGCTGGCGCGCAGAACCACGGGCa                                                                                       >  2:3906124/1‑150 (MQ=255)
                                                                gcTGGCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACG‑CCGTTACCGGGATCGATTCATCACATTCGGTGATGGT‑AAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGGAATAATGCTGGCGCGCAGAACCACGGACa                                                                                       >  1:1001101/1‑150 (MQ=255)
                                                                  tGGCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACG‑CCGTTACCGGGATCGATTCATCACATTCGGTGATGGT‑AAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACAtt                                                                                     <  1:3091060/150‑1 (MQ=255)
                                                                       ttCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACG‑CCGTTACCGGGATCGATTCATCACATTCGGTGATGGT‑AAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTg                                                                                >  1:1334982/1‑150 (MQ=255)
                                                                          ggTTTTGTCGGCTGGCACGCTGGCGGGCACGGACG‑CCGTTACCGGGGTCGATTCATCACATTCGGGGATGGT‑AAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTc                                                                             >  2:2211197/1‑150 (MQ=255)
                                                                          ggTTTTGTCGGCTGGCACGCTGGCGGGCACGGACG‑CCGTTACCGGGATCGATTCATCACATTCGGTGATGGT‑AAACCCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTc                                                                             <  1:638364/150‑1 (MQ=255)
                                                                          ggTTTTGTCGGCTGGCACGCTGGCGGGCACGGACG‑CCGTTACCGGGATCGATTCATCACATTCGGTGATGGT‑AAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTc                                                                             >  1:152015/1‑150 (MQ=255)
                                                                          ggTTTTGCCGGCTGGCACGCTGGCGGGCACGGACG‑CCGTTCCCGGGATCGATTCATCACATTCGGTGATGGT‑AAACCCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTc                                                                             <  1:2305528/150‑1 (MQ=255)
                                                                          ggTTTGGTCGGCTGGCACGCTGGCGGGCACGGACG‑CCGTTACCGGGATCGATTCATCACATTCGGTGATGGT‑AAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTc                                                                             <  1:979113/150‑1 (MQ=255)
                                                                               tGTCGGCGGGCCCGCTGGCGGGCACGGACG‑CCGTTCCCGGGATCGATTCATCACATTGGGTGATGGT‑AAACCCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTcgccgc                                                                        <  2:3787996/150‑1 (MQ=255)
                                                                                gTCGGCTGGCACGCGGGCGGGCACGGACG‑CCGTTCCCGGGATCGATTCACCACATTCGGTGATGAA‑AAACCCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCcgcg                                                                       <  2:2917044/150‑1 (MQ=255)
                                                                                gTCGGCGGGCCCGCGGGCGGGCACGGACG‑CCGTTCCCGGGATCGATTCATCACATGCGGTGATGGT‑AAACCCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCcgcg                                                                       <  2:3811700/150‑1 (MQ=255)
                                                                                      tGGCACGCTGGCGGGCACGGACG‑CCGTTACCGGGATCGATTCATCACATTCGGTGATGGT‑AAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCgtg                                                                 >  1:3246882/1‑150 (MQ=255)
                                                                                      tGGCACGCTGGCGGGCACGGACG‑CCGTTACCGGGATCGATTCATCACATTCGGGGATGGT‑AAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGGAATAATGCTGGCGCGCAGAACCACGGACATTGCCCGTTCGCCGCGCGCgtg                                                                 >  2:2948455/1‑150 (MQ=255)
                                                                                       ggCACGCTGGCGGGCACGGACG‑CCGTTACCGGGATCGATTCATCACATTCGGTGATGGT‑AAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCgtgt                                                                >  1:469290/1‑150 (MQ=255)
                                                                                        gCAGGCGGGCGGGCACGGACG‑CCGTTACCGGGATCGATTCATCACATTGGGTGATGGT‑AAACCCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGtgtg                                                               <  2:2077684/150‑1 (MQ=255)
                                                                                        gCACGCGGGGGGGCACGGACG‑CCGTTACCGGGATCGATTCACCACATTCGGTGATGGT‑AAACCCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGtgtg                                                               <  2:3438894/150‑1 (MQ=255)
                                                                                          aCGCTGGCGGGCACGGACG‑CCGTTACCGGGATCGATTCATCACATTCGGTGATGGT‑AAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGGAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCt                                                             >  2:802387/1‑150 (MQ=255)
                                                                                           cGCTGGCGGGCACGGACG‑CCGTTACCGGGATCGATTCATCACATTCGGTGATGGT‑AAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCtt                                                            >  2:121165/1‑150 (MQ=255)
                                                                                           cGCTGGCGGGAACGGACG‑CCGTTACGGGTTTCGTTTCCTCGATTTGGGTGAGGGT‑AAACCCCAGTAGGTCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCtt                                                            <  2:1019495/150‑1 (MQ=255)
                                                                                                    gCACGGCCG‑CCGTTCCCGGGATCGATTCATCACATTCGGGGATGGT‑AAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAatat                                                   <  2:3102578/150‑1 (MQ=255)
                                                                                                     cggggACG‑CCGTTACGGGGATCGTTTCATCCCTTTGGGTGATGGT‑AAACCCCAGAAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATc                                                  <  2:1987646/147‑1 (MQ=255)
                                                                                                      aCGGACG‑CCGTTCCCGGGATCGTTTCATCACATTCGGTGATGGT‑AAACCCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCa                                                 <  2:2008544/150‑1 (MQ=255)
                                                                                                         ggcG‑CCGTTACCGGGATCGATTCATCACATTGGGTGATGGT‑AAACCCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTc                                              <  2:2452387/148‑1 (MQ=255)
                                                                                                         gACG‑CCGTTACCGGGATCGATTCATCACATTCGGTGATGGT‑AAACCCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTc                                              <  2:82375/150‑1 (MQ=255)
                                                                                                         gACG‑CCGTTACCGGGATCGATTCATCACATTCGGTGATGGT‑AAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTc                                              >  2:908354/1‑150 (MQ=255)
                                                                                                         gACG‑CCGTTACCGGGATCGATTCATCACATTCGGTGATGGA‑AAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTc                                              <  1:2563753/150‑1 (MQ=255)
                                                                                                          aCG‑CCGTTACCGGGATCGATTCATCACATTCGGTGATGGT‑AAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCg                                             >  2:1421298/1‑150 (MQ=255)
                                                                                                               cGTTACCGGGATCGATTCATCACATTCGGTGATGGT‑AAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCtgt                                         >  2:184954/1‑150 (MQ=255)
                                                                                                                gtacccGGGTTCGATTCAGCACGTTCGGTGATGGT‑AAAACCAAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCtgtg                                        <  1:3186826/146‑1 (MQ=255)
                                                                                                                    cggggATCGATTCATCACATTCGGTGATGGT‑AAACCCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGtgatga                                    <  1:2887121/148‑1 (MQ=255)
                                                                                                                    ccGGGATCGATTCATCACATTCGGTGATGGT‑AAACCCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGtgatga                                    <  2:2374724/150‑1 (MQ=255)
                                                                                                                    ccGGGATCGATTCATCACATTCGGTGATGGT‑AAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGtgatga                                    >  2:541681/1‑150 (MQ=255)
                                                                                                                     cGGGCTCGATTCATCAAATTGGGGGATGGA‑AACACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAg                                   <  2:683895/150‑1 (MQ=255)
                                                                                                                      gggATCGATTCATCACATTCGGTGATGGT‑AAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAGt                                  >  1:3138244/1‑150 (MQ=255)
                                                                                                                       ggATCGATTCATCACATTCGGTGATGGT‑AAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAGtt                                 >  1:1623920/1‑150 (MQ=255)
                                                                                                                       ggATCGATTCATCACATTCGGTAATGGT‑AAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAGtt                                 <  1:1247620/150‑1 (MQ=255)
                                                                                                                         atggATTCATCACATTCGGTGATGGA‑AAACCCCAGAAGTTCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAGtttt                               <  1:1405741/147‑1 (MQ=255)
                                                                                                                         aTCGATTCATCACATTCGGTGATGGT‑AAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAGtttt                               >  2:3494540/1‑150 (MQ=255)
                                                                                                                         aTCGATTCATCACATTCGGTGATGGT‑AAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAGtttt                               >  1:3276056/1‑150 (MQ=255)
                                                                                                                         aTCGATTCATCACATTCGGTGATGGT‑AAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAGtttt                               >  1:2718520/1‑150 (MQ=255)
                                                                                                                          tcatttCATCACATTCGGTGATGGT‑AAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAGttttt                              <  2:2684531/146‑1 (MQ=255)
                                                                                                                            taTTCATCAAATTGGGTGGTGTT‑AAAGCCAAGTAGTCCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAGTTTTTTc                            <  1:3771994/149‑1 (MQ=255)
                                                                                                                            gtttCAACAAATTGGGGAATGGT‑AAACCCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCGCGAATATCATTCGCTGTGATGAGTTTTTTc                            <  2:3210405/148‑1 (MQ=255)
                                                                                                                             aTTCATCACATTCGGTGATGGT‑AAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAGTTTTTTCa                           >  1:714185/1‑150 (MQ=255)
                                                                                                                             aTTCATCACATTCGGTGATGGT‑AAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAGTTTTTTCa                           >  2:410344/1‑150 (MQ=255)
                                                                                                                                catcaCATTCGGTGATGGT‑AAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAGTTTTTTCACCt                        >  2:3391239/1‑150 (MQ=255)
                                                                                                                                caccactTTGGGGGATGAT‑AAACCCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAGTTTTTTCACCt                        <  2:1798878/147‑1 (MQ=255)
                                                                                                                                 atcaCATTCGGTGATGGT‑AAACCCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAGTTTTTTCACCTg                       <  1:1798189/150‑1 (MQ=255)
                                                                                                                                 atcaCATTCGGTGATGGT‑AAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAGTTTTTTCACCTg                       <  1:2806452/150‑1 (MQ=255)
                                                                                                                                 atcaCATTCGGTGATGGA‑AAACCCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAGTTTTTTCACCTg                       <  2:452084/150‑1 (MQ=255)
                                                                                                                                  tcaCATTGGTTGATGAT‑AAACCCCAGAGGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAGTTTTTTCACCTGc                      <  2:2791714/150‑1 (MQ=255)
                                                                                                                                   cacaTTCGGTGATGGT‑AAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAGTTTTTTCACCTGCt                     >  2:2126739/1‑150 (MQ=255)
                                                                                                                                      aTTGGGTGATGGT‑AAACCCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAGTTTTTTCACCTGCTtcc                  <  1:2438579/150‑1 (MQ=255)
                                                                                                                                      aTTCGGTGATGGT‑AAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAGTTTTTTCACCTGCTtcc                  >  1:2356393/1‑150 (MQ=255)
                                                                                                                                        tCGGTGATGGT‑AAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAGTTTTTTCACCTGCTtcctc                <  1:1295095/150‑1 (MQ=255)
                                                                                                                                          ggTGATGGT‑AAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAGTTTTTTCACCTGCTTcctcct              <  1:407113/150‑1 (MQ=255)
                                                                                                                                          ggTGATGGA‑AAACCCCGGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAGTTTTTTCACCTGCTTcctcct              <  2:2524921/150‑1 (MQ=255)
                                                                                                                                           gTGATGGT‑AAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAGTTTTTTCACCTGCTTCCTCCTg             <  1:37485/150‑1 (MQ=255)
                                                                                                                                               gggT‑AAACCCCGGTAGACCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAGTTTTTTCACCTGCTTCCTCCTGTTTg         <  2:3720193/149‑1 (MQ=255)
                                                                                                                                                 gT‑AAACCCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAGTTTTTTCACCTGCTTCCTCCTGTTTGGt       <  1:86423/150‑1 (MQ=255)
                                                                                                                                                    aaaaCCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAGTTTTTTCACCTGCTTCCTCCTGTTTGGTga     >  1:3704507/1‑150 (MQ=255)
                                                                                                                                                       aCCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAGTTTTTTCACCTGCTTCCTCCTGTTTGGTgagag  >  2:610671/1‑150 (MQ=255)
                                                                                                                                                       |                                                                                                                                                     
TGTGCGACCAGGCTTTCGGTAAACTGGCCTTCCGGCAGCTGGGCAATGATGGTTTCGCGGATGCGCTGGCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACG‑CCGTTACCGGGATCGATTCATCACATTCGGTGATGGT‑AAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAGTTTTTTCACCTGCTTCCTCCTGTTTGGTGAGAG  >  AP012306/2120356‑2120654

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑