Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123062,212,7530AC21.4% 386.8 / 33.1 145Y241D (TAT→GAT) hcaTpredicted 3‑phenylpropionic transporter
Reads supporting (aligned to +/- strand):  ref base A (105/9);  new base C (0/31);  total (105/40)
Fisher's exact test for biased strand distribution p-value = 7.11e-24
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 198 total aligned reads displayed.

CAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCC‑ACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAGCGCCAACCACGCAGACCAACC  >  AP012306/2212604‑2212897
                                                                                                                                                     |                                                                                                                                                 
cAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATa                                                                                                                                                   <  2:83268/150‑1 (MQ=255)
 aaCGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATAc                                                                                                                                                  <  2:1073645/150‑1 (MQ=255)
      cGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCC‑Ac                                                                                                                                            <  2:2443487/150‑1 (MQ=255)
       gTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCC‑Acc                                                                                                                                           >  2:3866675/1‑150 (MQ=255)
        tAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCC‑Accg                                                                                                                                          >  1:1001067/1‑150 (MQ=255)
        tAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCC‑Accg                                                                                                                                          <  1:639462/150‑1 (MQ=255)
         aGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCCGCGACCACAAATACCCC‑Accgc                                                                                                                                         >  2:51317/1‑150 (MQ=255)
         aGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCC‑Accgc                                                                                                                                         >  1:3514841/1‑150 (MQ=255)
             gCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCC‑ACCgccgag                                                                                                                                     >  2:88568/1‑150 (MQ=255)
                cccATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATCCCCC‑ACCgccgaggcc                                                                                                                                  <  2:772307/150‑1 (MQ=255)
                cccATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCC‑ACCgcccaggcc                                                                                                                                  >  2:3984612/1‑150 (MQ=255)
                   aTAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCC‑ACCgccgaggccgag                                                                                                                               >  1:755538/1‑150 (MQ=255)
                    tAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCC‑ACCccccaggcccagg                                                                                                                              >  2:1884281/1‑149 (MQ=255)
                      aTGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATCCCCC‑ACCGCCGAGGCCGAGTAg                                                                                                                            <  1:1769315/150‑1 (MQ=255)
                         ccccAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCCCAAATACCCC‑ACCGCCGAGGCCCAGTAGCCa                                                                                                                         >  2:3190783/1‑150 (MQ=255)
                            cAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTCCCGCCCCCCCGCCCAGCGACCACAAATCCCCC‑ACCGCCGAGGCCGAGTAGCCAGct                                                                                                                      <  2:197525/150‑1 (MQ=255)
                             aGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATCCCCC‑ACCGCCGAGGCCGAGTAGCCAGctg                                                                                                                     <  1:3735005/150‑1 (MQ=255)
                                  aCTACGCCGCAAATCGCCGAGATCAACAGCATACCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTCCCGCCACCACGCCCAGCGACCACAAATACCCC‑ACCGCCGAGGCCGAGTAGCCAGctgcctgc                                                                                                                <  1:3546847/150‑1 (MQ=255)
                                    tACGCCGCAAACCGCCGAGATCAACAGCATACCGCGTGCACTACACCGGCGAAAAATTTTATTCCTCAGCGCAAAGATAATGACTCCCGCCACCCCGCCCAGCGACCAAAAATCCCCC‑ACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCa                                                                                                              <  2:270671/150‑1 (MQ=255)
                                    tACCCCGCAAACCCCCGAGATCAACAGCATATCGCGTGCACCACAACGGGGGAAAAGTTTATTACCCAGCGAAAAGATAATGAGTTCCGCCACCCCGCCCAGCGACCACAAATCCCCC‑ACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCa                                                                                                              <  2:3026712/150‑1 (MQ=255)
                                      cgccgcAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCC‑ACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGt                                                                                                            >  1:1850298/1‑150 (MQ=255)
                                      cgccacAAACCGCCGAGATCAACAGAATCGCGCGTGCACTACACCGGCGAAAAAGTTAATTACTCAGCCCAAAGAAAATGACTCCCCCCCCCCCCCCCAGCGCCCAAAAATCCCCC‑CCCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGt                                                                                                            <  2:339335/150‑1 (MQ=255)
                                          gcAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGGGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCC‑ACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAAt                                                                                                        >  2:2218468/1‑150 (MQ=255)
                                            aaaTCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCC‑ACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATgg                                                                                                      >  1:2590858/1‑150 (MQ=255)
                                             aaTCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCC‑ACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGgc                                                                                                     >  1:2936426/1‑150 (MQ=255)
                                                  ccGAAATCAAAAGAATACCGCGTCCACTCCACCGGGGAAAAATTTTATTCCTCAGCGCAAAGATAATGACTCCCGCCCCCCCGCCCAGCGACCAAAAATCCCCC‑CCCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTaa                                                                                                <  2:3132247/150‑1 (MQ=255)
                                                   cGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCC‑ACCGCCGAAGCCGAGTAGCCAGCTGCCTGCCAGTAAAAGGCGCTaaa                                                                                               >  2:1928991/1‑150 (MQ=255)
                                                   cGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACCTCCGCCACCACGCCCAGCGACCACAAATACCCC‑CCCGCCGAGGGCGAGGAGCCAGCTGCCTGCCAGGAAAAGGCGCCaaa                                                                                               >  1:977179/1‑150 (MQ=255)
                                                    gagaTCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCC‑ACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTaaaa                                                                                              >  2:64357/1‑150 (MQ=255)
                                                     agaTCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCC‑ACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAAc                                                                                             >  1:935796/1‑150 (MQ=255)
                                                     agaTCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGAAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATCCCCC‑ACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAAc                                                                                             <  1:251582/150‑1 (MQ=255)
                                                      gaTCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCC‑ACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAAcc                                                                                            >  1:2834794/1‑150 (MQ=255)
                                                          aaCAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCC‑ACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTaa                                                                                        >  1:2108571/1‑150 (MQ=255)
                                                           aCATCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCC‑ACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAAt                                                                                       >  1:1981796/1‑150 (MQ=255)
                                                           aCAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCC‑ACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAAt                                                                                       >  1:1154457/1‑150 (MQ=255)
                                                           aCAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCC‑ACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAAt                                                                                       <  1:1482221/150‑1 (MQ=255)
                                                           aCAGCATACCGCGGGCACTACAACGGCGAAAAAGTTTATTCCCCAGCGCAAAGATAATGCCTTCCGCCCCCACGCCCAGCGACCACAAATCCCCC‑ACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAAt                                                                                       <  2:1678944/150‑1 (MQ=255)
                                                             aGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCC‑ACCGCCGAGGCCCAGTAGCCAGCTGCCCGCCAGTAAAAGGCGCTAAAACCGGAATAg                                                                                     >  2:2945694/1‑150 (MQ=255)
                                                              gCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCC‑ACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAgg                                                                                    >  2:2342426/1‑150 (MQ=255)
                                                              gCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCC‑ACCGCCGAGGCCGAGAAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAgg                                                                                    >  1:81541/1‑150 (MQ=255)
                                                                atatCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCC‑ACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGcc                                                                                  >  1:3404963/1‑150 (MQ=255)
                                                                atatCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCC‑ACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGcc                                                                                  >  2:3325590/1‑150 (MQ=255)
                                                                atatCGCGTGCACTACAACGGCGAAAAATTTTATTCCCCAGCGCAAAGATAATGACTTCCGCCACCACGCCCGGCGCCCACAAATCCCCC‑ACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGcc                                                                                  <  2:708938/150‑1 (MQ=255)
                                                                atacCGCGTCCCCTACAACGGCGAAAAATTTTATTCCTCAGCGCAAAGATAATGCCTCCCGCCACCACGCCCACCGCCCAAAAACCCCCC‑ACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGcc                                                                                  <  2:76502/150‑1 (MQ=255)
                                                                     gcgTGCACAACACCGGCGAAAAATTTTATTACCCAGCGCAAAGATATTCCCTTCCTCCACCAGGCCAGGCAACCACAAATACCCC‑GCCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCAtg                                                                             <  2:1557313/150‑1 (MQ=255)
                                                                      cgTGCACTCCAACGGGGAAAAATTTTATTCCTCAGCGCAAAAATAATGACTCCCGCCACCCCGCCCAGCGACCACAAATCCCCC‑CCCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCAtgt                                                                            <  2:2292138/150‑1 (MQ=255)
                                                                        tGCACTACAACGGCGGAAAAGATTATTACTCAGCGCAAAGATAAAGGCTTTCACCCCCAACCCCAGCGACCACAAATACCCC‑ACCGCCGGGGCCCAGTGGCCAGCTGCCTGCCAGTAAAAGCCGCTAAAACCGTAACACGCCGCATGTGc                                                                          >  2:1526735/1‑150 (MQ=255)
                                                                              acacCGGCGAAAAATTTTATTCCTCAGCGAAAAGATAATGCCTCCCCCCCCCCCGCCCAGCGACCACAAATCCCCC‑CCCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTg                                                                    <  2:2596456/150‑1 (MQ=255)
                                                                                accggcgaAAAATTTTATTACTCAGCACAAAGAAAATACCTCCCGCCACCACGCCAAGCGACCACAAATCCCCC‑ACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCa                                                                  <  2:3488251/148‑1 (MQ=255)
                                                                                 aCGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCC‑ACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCaa                                                                 >  2:1832954/1‑150 (MQ=255)
                                                                                 aCGGCGAAAAAGTTTATTACCCAGCGCAAAGAAAATGACTCCCGCCACCACCCCCAGCGCCCACAAACCCCCC‑ACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCaa                                                                 <  2:212754/150‑1 (MQ=255)
                                                                                  cggcggAAAAGTTTATTACTCAGCGAAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATCCCCC‑ACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAAt                                                                <  1:3221540/150‑1 (MQ=255)
                                                                                    gcggAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCC‑ACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATaa                                                              >  1:3491773/1‑150 (MQ=255)
                                                                                    gcgaAAAATTTTATTACTCAGCGAAAAGATAATGCCTCCCGCCACCACGCCCAGCGACCACAAATACCCC‑ACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATaa                                                              <  1:2741692/150‑1 (MQ=255)
                                                                                     cggAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCC‑ACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATaaa                                                             >  1:2648452/1‑150 (MQ=255)
                                                                                       gAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCC‑ACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATaaaca                                                           >  2:410636/1‑150 (MQ=255)
                                                                                       gAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCC‑ACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATaaaca                                                           >  1:1684083/1‑150 (MQ=255)
                                                                                        aaaaGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCC‑ACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATaaacaa                                                          >  2:1268073/1‑150 (MQ=255)
                                                                                        aaaaGTTTATACCCCAGCCAAAAGAAAAGGCCTCCCGCCACCACGCCCAGCGACCAAAAATCCCCC‑ACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATaaacaa                                                          <  2:3540326/150‑1 (MQ=255)
                                                                                        aaaaGTTCATTCCTCAGCGCAAAGATAATGACTCCCGCCACCACGCCCAGCGACCACAAATCCCCC‑ACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATaaacaa                                                          <  2:3171403/150‑1 (MQ=255)
                                                                                         aaaTTTTATTACCCAGCGAAAAAAAAATGACTCCCGCCCCCCCCCCAAGCGACCACAAACCCCCC‑ACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATaaacaaa                                                         <  2:26723/150‑1 (MQ=255)
                                                                                           aGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCC‑ACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACg                                                       >  2:2018918/1‑150 (MQ=255)
                                                                                           aGTTTATTACACAGCGAAAAGAAAATGACTCCCGCCCCCACGCCCAGCGCCCACAAACCCCCC‑ACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACg                                                       <  2:3098484/150‑1 (MQ=255)
                                                                                              ttattaCTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCC‑ACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAg                                                    >  1:705775/1‑150 (MQ=255)
                                                                                                attaCTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCC‑ACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGc                                                  >  1:1711567/1‑150 (MQ=255)
                                                                                                attaCTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCC‑ACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGc                                                  >  2:630776/1‑150 (MQ=255)
                                                                                                 ttaCTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCC‑ACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGcc                                                 >  2:610683/1‑150 (MQ=255)
                                                                                                  taCTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCC‑ACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCa                                                >  2:3154395/1‑150 (MQ=255)
                                                                                                   aCTCAGCGCAAAGATAATGCCTCCCGCCACCCCGCCCAGCGACCAAAAATACCCC‑ACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAg                                               <  1:1287165/150‑1 (MQ=255)
                                                                                                   aCTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCC‑ACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAg                                               >  2:3751540/1‑150 (MQ=255)
                                                                                                    cTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCC‑ACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGGCAGa                                              >  1:3301376/1‑150 (MQ=255)
                                                                                                      cAGCGCAAGGATAATGACTTCCGCCCCCACGCCCAGCGACCACAAATCCCCC‑ACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGaaa                                            <  1:613971/150‑1 (MQ=255)
                                                                                                      cAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCC‑ACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGaaa                                            >  1:2555988/1‑150 (MQ=255)
                                                                                                      cAGCACAAAGATAAGGCCTTCCCCCACCCCGCCCAGCGCCAACAAATCCCCCTACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGaa                                             <  2:492056/150‑1 (MQ=255)
                                                                                                        gcgaAAAAAAAATTACTCCCGCCACCCTGCCCAGCGACCCCAACTCCCCC‑ACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAgc                                          <  1:1018335/150‑1 (MQ=255)
                                                                                                          gcAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCC‑ACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAgcgc                                        >  1:3257762/1‑150 (MQ=255)
                                                                                                            aaaaATAATGACTCCCGCCACCCCCCCCAGCGACCACAAATCCCCC‑ACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCa                                      <  2:2471984/150‑1 (MQ=255)
                                                                                                            aaaGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCC‑ACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCa                                      >  2:2993492/1‑150 (MQ=255)
                                                                                                             aaGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCC‑ACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAg                                     >  1:1887818/1‑150 (MQ=255)
                                                                                                                 tAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCC‑ACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCt                                 >  1:175650/1‑150 (MQ=255)
                                                                                                                 tAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCC‑ACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCt                                 >  1:3102495/1‑150 (MQ=255)
                                                                                                                 aaaggccTTCCGCCCCCACCCCCAGCAACCAAAAACCCCCC‑CCCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCt                                 <  2:828967/144‑1 (MQ=255)
                                                                                                                  aaTGACTCCCCCCCCCCCCCCCAGCGACCAAAAATCCCCC‑ACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTg                                <  2:1369599/150‑1 (MQ=255)
                                                                                                                     gACTTCCGCCACCACGCCCAGCGACCACAAATACCCC‑ACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCg                             >  2:1608474/1‑150 (MQ=255)
                                                                                                                     gACTTCCGCCACCACGCCCAGCGACCACAAATACCCC‑ACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCg                             >  2:3523715/1‑150 (MQ=255)
                                                                                                                     gACTCCCGCCACCACGCCCAGCGACCACAAATACCCC‑ACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCg                             <  1:462930/150‑1 (MQ=255)
                                                                                                                       cTTCCGCCACCACGCCCAGCGACCACAAATACCCC‑ACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGaa                           >  1:2292077/1‑150 (MQ=255)
                                                                                                                         tCCGCCACCACGCCCAGCGACCACAAATACCCC‑ACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAAcc                         >  1:1208739/1‑150 (MQ=255)
                                                                                                                         tCCGCCACCACGCCCAGCGACCACAAATACCCC‑ACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGGGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAAcc                         >  2:1014326/1‑150 (MQ=255)
                                                                                                                         cccGCCCCCCCGCCCGGCGACAAAAAATCCCCC‑CCCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAAcc                         <  2:940157/149‑1 (MQ=255)
                                                                                                                          ccGCCACCACGCCCAGCGACCACAAATCCCCC‑ACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCa                        <  1:199065/150‑1 (MQ=255)
                                                                                                                            gCCACCACGCCCAGCGACCACAAATACCCC‑ACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAgc                      >  1:2442644/1‑150 (MQ=255)
                                                                                                                              caccacGCCCAGCGACCACAAATACCCC‑ACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAgcgc                    >  1:139194/1‑150 (MQ=255)
                                                                                                                                  ccGCCCAGCGACCACAAATCCCCC‑ACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAGCGCCAAc                <  1:1606243/149‑1 (MQ=255)
                                                                                                                                    gCCAAGCGACAACAAACCCCCC‑ACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAGCGCCAACCa              <  1:3958137/150‑1 (MQ=255)
                                                                                                                                     cccAGCGACCACAAATACCCC‑ACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAGCGCCAACCAc             >  1:2420988/1‑150 (MQ=255)
                                                                                                                                       cAGCGACCACAAATACCCC‑ACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAGCGCCAACCACGc           >  2:3260398/1‑150 (MQ=255)
                                                                                                                                          cgcccAAAAATCCCCC‑CCCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAGCGCCAACCACGCAGa        <  2:2548386/147‑1 (MQ=255)
                                                                                                                                              cacaAATACCCC‑ACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAGCGCCAACCACGCAGACCaa    >  1:2334756/1‑150 (MQ=255)
                                                                                                                                                aaaaTCCCCC‑ACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAGCGCCAACCACGCAGACCAAcc  <  2:1646898/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
CAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCC‑ACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAGCGCCAACCACGCAGACCAACC  >  AP012306/2212604‑2212897

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑