Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123062,714,5550TG25.0% 740.9 / 27.5 308T175P (ACC→CCC) ygjVconserved inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (73/158);  new base G (77/0);  total (150/158)
Fisher's exact test for biased strand distribution p-value = 1.01e-30
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 347 total aligned reads displayed.

GCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGG‑CGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTT  >  AP012306/2714406‑2714703
                                                                                                                                                      |                                                                                                                                                    
gCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGG‑CGGAAGGGGt                                                                                                                                                      <  2:725599/150‑1 (MQ=255)
  gTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGG‑CGGAAGGGGttt                                                                                                                                                    >  1:1377947/1‑150 (MQ=255)
  gTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGG‑CGGAAGGGGgtt                                                                                                                                                    >  2:1935697/1‑150 (MQ=255)
   tATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGG‑CGGAAGGGGtttt                                                                                                                                                   <  1:1613887/150‑1 (MQ=255)
   tATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGG‑CGGAAGGGGgttt                                                                                                                                                   >  2:45122/1‑150 (MQ=255)
   tATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGG‑CGGAAGGGGgttt                                                                                                                                                   >  2:2209135/1‑150 (MQ=255)
    aTTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGG‑CGGAAGGGGTTTtc                                                                                                                                                  >  2:1350331/1‑150 (MQ=255)
    aTTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGG‑CGGAAGGGGGTTtc                                                                                                                                                  >  1:3215209/1‑150 (MQ=255)
      tGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGG‑CGGAAGGGGTTTtctc                                                                                                                                                <  2:1218510/150‑1 (MQ=255)
       gCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGG‑CGGAAGGGGTTTTCTCa                                                                                                                                               <  1:1195857/150‑1 (MQ=255)
        cAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGG‑CGGAAGGGGTTTTCTCaa                                                                                                                                              <  1:2678982/150‑1 (MQ=255)
        cAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGG‑CGGAAGGGGTTTTCTCaa                                                                                                                                              <  2:3780561/150‑1 (MQ=255)
        cAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGG‑CGGAAGGGGGTTTCTCaa                                                                                                                                              >  2:2784640/1‑150 (MQ=255)
        cAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCG‑CCTGCGGAATTTATTGGTTAACCCCTTTCGGCTACGGCCGGAAGGGGTTTTTTCac                                                                                                                                              >  1:1445932/1‑149 (MQ=255)
         aGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGG‑CGGAAGGGGGTTTCTCAAc                                                                                                                                             >  1:313524/1‑150 (MQ=255)
         aGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGG‑CGGAAGGGGGTTTCTCAAc                                                                                                                                             >  1:1968953/1‑150 (MQ=255)
              tctTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGG‑CGGAAGGGGGTTTTTCAACTTTaa                                                                                                                                        >  2:864460/1‑150 (MQ=255)
              tctTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTCTAACCCCTTTCGTCTACGG‑CGGAAAGGGGTTTCTCAACtttta                                                                                                                                        >  2:4002231/1‑148 (MQ=255)
               ctTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGG‑CGGAAAGGGGTTTTTCAACTTTaaa                                                                                                                                       >  2:3435794/1‑150 (MQ=255)
                  gCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGG‑CGGAAGGGGTTTTCTCAACTTTAAACgg                                                                                                                                    <  2:3107608/150‑1 (MQ=255)
                   cGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGG‑CGGAAGGGGTTTTCTCAACTTTAAACGGa                                                                                                                                   <  2:1746898/150‑1 (MQ=255)
                    gAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGG‑CGGAAGGGGGTTTCTCAACTTTAAACGGAt                                                                                                                                  >  2:2402983/1‑150 (MQ=255)
                    gAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGG‑CGGAAGGGGGCTTCTCAACTTTAAACGGAt                                                                                                                                  >  1:2108014/1‑150 (MQ=255)
                     aaaCCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGG‑CGGAAGGGGTTTTCTCAACTTTAAACGGATc                                                                                                                                 <  2:2912827/150‑1 (MQ=255)
                     aaaCCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGG‑CGGAAGGGGTTTTCTCAACTTTAAACGGATc                                                                                                                                 >  2:1035412/1‑150 (MQ=255)
                     aaaCCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGG‑CGGAAGGGGTTTTCTCAACTTTAAACGGATc                                                                                                                                 <  2:1712096/150‑1 (MQ=255)
                       aCCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGG‑CGGAAGGGGTTTTCTCAACTTTAAACGGATCaa                                                                                                                               <  1:2201166/150‑1 (MQ=255)
                        ccGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGG‑CGGAAGGGGTTTTCTCAACTTTAAACGGATCAAt                                                                                                                              <  2:2853246/150‑1 (MQ=255)
                          gcgcTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCATGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGG‑CGGAAGGGGGTTTCTCAACTTTAAAAGGATCAATTc                                                                                                                            >  2:3473302/1‑150 (MQ=255)
                           cgcTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGG‑CGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTcc                                                                                                                           <  1:849153/150‑1 (MQ=255)
                           cgcTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGG‑CGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTcc                                                                                                                           <  2:910792/150‑1 (MQ=255)
                             cTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGG‑CGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTcccc                                                                                                                         <  1:3176491/150‑1 (MQ=255)
                             cTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGG‑CGGAAGGGGGTTTCTCAACTTTAAACGGATCAATTcccc                                                                                                                         >  1:496055/1‑150 (MQ=255)
                                aaCTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGG‑CGGAAGGGGGTTTCTCAACTTTAAACGGATCAATTCCCCctt                                                                                                                      >  2:300042/1‑150 (MQ=255)
                                   tctTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGG‑CGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCt                                                                                                                   <  1:2891671/150‑1 (MQ=255)
                                               gTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGG‑CGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGa                                                                                                       >  1:2828725/1‑150 (MQ=255)
                                               gTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTAGTCTACGG‑CGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGa                                                                                                       <  2:1264907/150‑1 (MQ=255)
                                                tGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGG‑CGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGaa                                                                                                      >  1:3098265/1‑150 (MQ=255)
                                                tGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGG‑CGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGaa                                                                                                      >  1:462923/1‑150 (MQ=255)
                                                   tGTTCACTGCGGCAGCTTGCCAGTCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGG‑CGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACg                                                                                                   <  2:3592662/150‑1 (MQ=255)
                                                   tGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGG‑CGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACg                                                                                                   <  1:1783917/150‑1 (MQ=255)
                                                    gTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGG‑CGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGa                                                                                                  >  1:1896580/1‑150 (MQ=255)
                                                        aCTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGG‑CGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGa                                                                                              >  1:2767742/1‑150 (MQ=255)
                                                            cGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGG‑CGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATAtt                                                                                          <  1:2941151/150‑1 (MQ=255)
                                                             ggCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGG‑CGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTc                                                                                         <  1:2303423/150‑1 (MQ=255)
                                                               cAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGG‑CGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAg                                                                                       <  1:1032228/150‑1 (MQ=255)
                                                                      ccAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGG‑CGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTc                                                                                <  1:1653468/150‑1 (MQ=255)
                                                                         ggCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGG‑CGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATa                                                                             <  1:1305809/150‑1 (MQ=255)
                                                                         ggCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGG‑CGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATa                                                                             <  1:996936/150‑1 (MQ=255)
                                                                          gCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGG‑CGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATaa                                                                            <  1:890315/150‑1 (MQ=255)
                                                                          gCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGG‑CGGAAGGGGGTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATaa                                                                            >  1:2156921/1‑150 (MQ=255)
                                                                           cAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGG‑CGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAg                                                                           <  2:376712/150‑1 (MQ=255)
                                                                            agaagaCGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGG‑CGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGc                                                                          <  2:3698744/150‑1 (MQ=255)
                                                                            agaagaCGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGG‑CGGAAGGGGGTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCCTTCATAAGc                                                                          >  1:3961927/1‑150 (MQ=255)
                                                                             gaagaCGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGG‑CGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCa                                                                         >  1:2591363/1‑150 (MQ=255)
                                                                               agaCGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGG‑CGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGa                                                                       <  1:1225388/150‑1 (MQ=255)
                                                                                 aCGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGG‑CGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGaaa                                                                     >  1:3649675/1‑150 (MQ=255)
                                                                                 aCGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGG‑CGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGaaa                                                                     <  1:1322109/150‑1 (MQ=255)
                                                                                 aCGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGG‑CGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGaaa                                                                     >  2:3835979/1‑150 (MQ=255)
                                                                                  cGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGG‑CGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGaaaa                                                                    <  2:565399/150‑1 (MQ=255)
                                                                                  cGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGG‑CGGAAGGGGGTTTCTCAAATTTAAACGGATCAATTCCCCTTTTTTGCATCCGCCCGAAACGAATGGTATTCAGGCCCTTCCTAAGCAGaaaa                                                                    >  2:3346007/1‑150 (MQ=255)
                                                                                   gATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGG‑CGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAAc                                                                   <  2:1076349/150‑1 (MQ=255)
                                                                                    aTCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGG‑CGGAAGGGGGTTTCTCAACTTTAAACGGGTCAATTCCCCTTTTCTTCCTCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACt                                                                  >  2:1812399/1‑150 (MQ=255)
                                                                                       gTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGG‑CGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTAcc                                                               >  2:3162093/1‑150 (MQ=255)
                                                                                       gTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGG‑CGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTAcc                                                               >  1:415965/1‑150 (MQ=255)
                                                                                        tCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGG‑CGGAAGGGGGTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAAGCCATTCATAAGCAGAAAACTAccc                                                              >  1:906260/1‑150 (MQ=255)
                                                                                         cTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGG‑CGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCt                                                             <  1:2871248/150‑1 (MQ=255)
                                                                                         cTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGG‑CGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCt                                                             <  2:2475838/150‑1 (MQ=255)
                                                                                          tGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGG‑CTGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTc                                                            >  1:1847722/1‑150 (MQ=255)
                                                                                          tGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGG‑CGGAAGGGGGTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTc                                                            >  2:2727009/1‑150 (MQ=255)
                                                                                             cAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGG‑CGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAat                                                         <  2:1529092/150‑1 (MQ=255)
                                                                                             cAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGG‑CGGAAGGGGGTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAat                                                         >  2:1427996/1‑150 (MQ=255)
                                                                                               aaTAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGG‑CGGAAGGGGGTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAatca                                                       >  1:74918/1‑150 (MQ=255)
                                                                                                aTAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGG‑CGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAatcat                                                      <  1:1555696/150‑1 (MQ=255)
                                                                                                  aGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGG‑CGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAATCATCg                                                    <  2:2322909/150‑1 (MQ=255)
                                                                                                   gcgcCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGG‑CGGAAGGGGGTTTCTCAACTTTAAACGGATCAATTCCCCCTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAATCATCGt                                                   >  1:3908359/1‑150 (MQ=255)
                                                                                                      cccTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGG‑CGGAAGGGGTTTTTTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCAATCATAAGCAGAAAACTACCCTCAATCATCGTgcc                                                >  2:3892244/1‑150 (MQ=255)
                                                                                                        cTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGG‑CGGAAGGGGGTTTCTCAACTTTAAACGGATCAATTTCCCTTTTCTGCATCCCCCAGAAACGAATGATATTCACGCCATTCATAAGAAGAAAACTACCCTCAATCATCCTgccgc                                              >  2:3318866/1‑150 (MQ=255)
                                                                                                              aattaattGTTTACCCCCTTTCGTCTACGG‑CGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTGCCCTCAATCATCGTGCCGCCTATCg                                        <  1:3439030/150‑1 (MQ=255)
                                                                                                              aattaattGTTTAACCCCTTTCGTCTACGG‑CGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAATCATCGTGCCGCCTATCg                                        <  1:1119257/150‑1 (MQ=255)
                                                                                                               attaattGTTTAACCCCTTTCGTCTACGG‑CGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAATCATCGTGCCGCCTATCGa                                       >  1:1535492/1‑150 (MQ=255)
                                                                                                                  aattGTTTAACCCCTTTCGTCTACGG‑CGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAATCATCGTGCCGCCTATCGAccc                                    >  1:2391214/1‑150 (MQ=255)
                                                                                                                   attGTTTAACCCCTTTCGTCTACGG‑CGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAATCATCGTGCCGCCTATCGAcccc                                   <  1:3266245/150‑1 (MQ=255)
                                                                                                                       tttAACCCCTTTCGTCTACGG‑CGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAATCATCGTGCCGCCTATCGACCCCGccc                               >  1:3862775/1‑150 (MQ=255)
                                                                                                                          aaCCCCTTTCGTCTACGG‑CGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAATCATCGTGCCGCCTATCGACCCCGCCCAGa                            <  2:1653015/150‑1 (MQ=255)
                                                                                                                          aaCCCCTTTCGTCTACGG‑CGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAATCATCGTGCCGCCTATCGACCCCGCCCAGa                            <  1:3045655/150‑1 (MQ=255)
                                                                                                                           aCCCCTTTCGTCTACGG‑CGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAATCATCGTGCCGCCTATCGACCCCGCCCAGaa                           >  1:3773030/1‑150 (MQ=255)
                                                                                                                                 ttCGTCTACGG‑CGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGTtgtg                     >  2:1090535/1‑150 (MQ=255)
                                                                                                                                 ttCGTCTACGG‑CGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAAAGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGTtgtg                     >  2:1559502/1‑150 (MQ=255)
                                                                                                                                   cGTCTACGG‑CGGAAGGGGGTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGATGTGaa                   >  2:2254776/1‑150 (MQ=255)
                                                                                                                                    gTCTACGG‑CGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGTTGTGAAt                  >  1:615265/1‑150 (MQ=255)
                                                                                                                                    gTCTACGG‑CGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGTTGTGAAt                  <  1:1155101/150‑1 (MQ=255)
                                                                                                                                     tCTACGG‑CGGAAGGGGGTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGGCATTCATAAGCAGAAAACTACCCTCAATCATCGTGCCGCCTATCGACCCCGCCCCGAAGTTGTGAATc                 >  2:2130407/1‑150 (MQ=255)
                                                                                                                                      cTACGG‑CGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGTTGTGAATCa                <  1:2631270/150‑1 (MQ=255)
                                                                                                                                        aCGG‑CGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGTTGTGAATCAcc              >  2:1494711/1‑150 (MQ=255)
                                                                                                                                          gg‑cggAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGTTGTGAATCACCCa            >  1:2479587/1‑150 (MQ=255)
                                                                                                                                           g‑cggAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGTTGTGAATCACCCAg           <  2:2606124/150‑1 (MQ=255)
                                                                                                                                              ggAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCa         >  2:1292544/1‑150 (MQ=255)
                                                                                                                                                    ggTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGt   >  2:2054145/1‑150 (MQ=255)
                                                                                                                                                     gTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGtt  <  1:353958/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                    
GCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGG‑CGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTCTGCATCCGCCAGAAACGAATGATATTCAGGCCATTCATAAGCAGAAAACTACCCTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTT  >  AP012306/2714406‑2714703

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑