Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123063,527,3680AC27.0% 624.6 / 55.0 267L140F (TTA→TTC) rhaSDNA‑binding transcriptional activator, L‑rhamnose‑binding
Reads supporting (aligned to +/- strand):  ref base A (162/33);  new base C (0/72);  total (162/105)
Fisher's exact test for biased strand distribution p-value = 9.43e-40
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 304 total aligned reads displayed.

CGATTTCAGTTTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAGGTGAATTG  >  AP012306/3527219‑3527517
                                                                                                                                                     |                                                                                                                                                     
cGATTTCAGTTTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTa                                                                                                                                                       <  1:1966928/150‑1 (MQ=255)
 gATTTCAGTTTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTAc                                                                                                                                                      <  1:3118457/150‑1 (MQ=255)
   tttCAGTTTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTAccc                                                                                                                                                    >  2:1509982/1‑150 (MQ=255)
    ttCAGTTTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCt                                                                                                                                                   <  1:2264243/150‑1 (MQ=255)
    ttCAGTTTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCt                                                                                                                                                   <  1:1499846/150‑1 (MQ=255)
     tCAGTTTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTc                                                                                                                                                  <  1:132643/150‑1 (MQ=255)
       aGTTTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGa                                                                                                                                                >  2:3488056/1‑150 (MQ=255)
          ttCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCg                                                                                                                                             <  2:2179711/150‑1 (MQ=255)
           tctcGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGc                                                                                                                                            >  2:2394119/1‑150 (MQ=255)
            ctcGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGcc                                                                                                                                           >  1:543484/1‑150 (MQ=255)
                 cGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTcg                                                                                                                                      >  2:1400945/1‑150 (MQ=255)
                  gggCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTcgc                                                                                                                                     >  2:3697941/1‑150 (MQ=255)
                   ggCGGAACCAGTTGCAGCCACAAGACCTGGATGGACACTACCCGTCTCACGGGCTCGTTAACCACATCGTATGGCAGCAGGTGCGACACCTTGTTGCACAGATGAAACGGCAGAAGGGGAAAAATGATTACCCCTCGACCGCCAGTcgcg                                                                                                                                    <  2:3771240/150‑1 (MQ=255)
                    gCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCga                                                                                                                                   >  1:274727/1‑150 (MQ=255)
                        aaTCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATc                                                                                                                               >  1:121149/1‑150 (MQ=255)
                            ggTTGCTGCCACAAGAGCGGGAGGGGCAGTATCCGTCTCCCTGGCGCGTTAACCACAGCGTATGGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAGGGGAAAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGt                                                                                                                           <  2:2900828/149‑1 (MQ=255)
                            ggTTGCTGCCAAAAGGGCTGGAGGGGCAGTATCCGTCTCACTGGCGCTTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGAGGGAACAGAAGGAGGGGAAAAATGTTTTCCCCTCGACCGCCAGTCGCGAGATCTTGt                                                                                                                           <  2:3836483/149‑1 (MQ=255)
                            aGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGt                                                                                                                           >  1:2909455/1‑150 (MQ=255)
                               cgctgcCAAAAGAGGTGGAGGGGCGGTTTCCTTCACCCTGGCGCTTTAACCACGGGGTATTGCAGAAGGTGCGAAAGCGGGTTGCACAGATGAAACAGCAGGAAGGGAAAAATATTTTACCCCCGACCGCCAGTCGCGAGATCTTGTTTa                                                                                                                        <  2:605979/149‑1 (MQ=255)
                                gctgcCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTAt                                                                                                                       >  1:1515545/1‑150 (MQ=255)
                                 ctgcCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAGGGGGAAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATg                                                                                                                      <  1:2613094/150‑1 (MQ=255)
                                  tgcCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGc                                                                                                                     <  2:3342207/150‑1 (MQ=255)
                                  tgcCAAAAGAGCTGGATGGGCAGTACCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGc                                                                                                                     <  1:1666785/150‑1 (MQ=255)
                                      acaAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAAtt                                                                                                                 >  1:1908210/1‑150 (MQ=255)
                                       caAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTACCCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTa                                                                                                                <  1:3725600/150‑1 (MQ=255)
                                        aaGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTAc                                                                                                               >  2:3901846/1‑150 (MQ=255)
                                         agagCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACt                                                                                                              <  1:2990978/150‑1 (MQ=255)
                                          gagCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTg                                                                                                             >  2:3045372/1‑150 (MQ=255)
                                           agCTGGATGGGCAGTATCCGTCTCCCTGGCGCGTTAACCACAGCTTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGAAAAATTATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGc                                                                                                            <  2:3647503/150‑1 (MQ=255)
                                                  tGGGCAGTACCCGTCTCACGGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGAGGGAACAGCAGGAAGGGAAAAATGTTTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTtgct                                                                                                     <  2:2128000/150‑1 (MQ=255)
                                                   gggCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTtgctg                                                                                                    >  2:1661200/1‑150 (MQ=255)
                                                     gCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCg                                                                                                  >  2:1905782/1‑150 (MQ=255)
                                                     gCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCg                                                                                                  >  1:926934/1‑150 (MQ=255)
                                                      cAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGt                                                                                                 >  2:3140033/1‑150 (MQ=255)
                                                      cAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGt                                                                                                 >  1:3991305/1‑150 (MQ=255)
                                                          aTCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTaaaa                                                                                             >  1:1446440/1‑150 (MQ=255)
                                                          aTCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTaaaa                                                                                             >  1:3502014/1‑150 (MQ=255)
                                                            ccGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGc                                                                                           <  1:3099798/150‑1 (MQ=255)
                                                            ccGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGc                                                                                           >  2:1837125/1‑150 (MQ=255)
                                                            ccGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGc                                                                                           >  1:1164261/1‑150 (MQ=255)
                                                              gTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAg                                                                                         >  2:2554800/1‑150 (MQ=255)
                                                                ctcACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGAAAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGtt                                                                                       <  2:3253902/150‑1 (MQ=255)
                                                                   aCTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGc                                                                                    >  2:471082/1‑150 (MQ=255)
                                                                   aCTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGc                                                                                    >  1:994050/1‑150 (MQ=255)
                                                                    cTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCa                                                                                   <  1:2686843/150‑1 (MQ=255)
                                                                      ggCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAgg                                                                                 <  2:762775/150‑1 (MQ=255)
                                                                      ggCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGAAAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAgg                                                                                 <  2:541994/150‑1 (MQ=255)
                                                                        cgcgTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGgag                                                                               >  2:1587158/1‑150 (MQ=255)
                                                                          cgTTACCCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGAAAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGaa                                                                             <  2:1293338/150‑1 (MQ=255)
                                                                          cgTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGaa                                                                             <  1:825920/150‑1 (MQ=255)
                                                                          cgTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGaa                                                                             >  1:744018/1‑150 (MQ=255)
                                                                            ttAACCACAGCGTATTGCAGCAGGTGCGACAGGTGGTTGCAAAGGTGAAACAGCAGGAGGGGAAAAATGATTTGCCCTCGTCCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAAcc                                                                           <  2:3582220/150‑1 (MQ=255)
                                                                             tAACCACAGCGTATTGCAGCAGGTGCGACAGTTGGTTGCACAGATGGAACAGCAGGAGGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCt                                                                          <  1:3370817/150‑1 (MQ=255)
                                                                             tAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAGGGGAAAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCt                                                                          <  1:1024176/150‑1 (MQ=255)
                                                                               aCCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTgg                                                                        >  1:3548608/1‑150 (MQ=255)
                                                                                   caGCGTTTTGCGGCGGGTGCGACAGCTGGTTGCACAGATGGAACAGAAGGAAGGGAAAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGaaaa                                                                    <  2:2361809/150‑1 (MQ=255)
                                                                                   caGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGaaaa                                                                    >  2:2272748/1‑150 (MQ=255)
                                                                                     gCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACa                                                                  <  1:1122619/150‑1 (MQ=255)
                                                                                      cGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAg                                                                 >  1:491951/1‑150 (MQ=255)
                                                                                      cGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTACCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAg                                                                 <  1:185153/150‑1 (MQ=255)
                                                                                        tATTGCAGCAGGGGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAggg                                                               >  2:3518518/1‑148 (MQ=255)
                                                                                           tGCAGCAGGTGCGACGGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCAt                                                            <  2:2729940/150‑1 (MQ=255)
                                                                                           tGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCAt                                                            <  2:1999278/150‑1 (MQ=255)
                                                                                              agcagGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCAc                                                         >  1:3800118/1‑150 (MQ=255)
                                                                                               gcagGTGCGACGCCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACg                                                        <  2:3172965/150‑1 (MQ=255)
                                                                                                cagGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGt                                                       >  1:1249225/1‑150 (MQ=255)
                                                                                                    tGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCCCGTCTCa                                                   <  1:3291663/150‑1 (MQ=255)
                                                                                                       gACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACt                                                >  2:2412093/1‑150 (MQ=255)
                                                                                                           gCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCt                                            >  2:2014341/1‑150 (MQ=255)
                                                                                                           gCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCt                                            >  1:1602717/1‑150 (MQ=255)
                                                                                                           gCTGGTGGCACAGATGGAACAGCGGGAAGGGGAAAATGATTAACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCt                                            <  1:2826536/150‑1 (MQ=255)
                                                                                                              ggatGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGCCGCGAGATCTTGTTTCTGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAACCAGCGCATCACGTCTCAACTTGCTTct                                         >  2:2618271/4‑150 (MQ=255)
                                                                                                              ggTTGCACAGCTGGAACAGCAGAAAGGGAAAATTGTTTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTct                                         <  1:3940052/150‑1 (MQ=255)
                                                                                                              ggTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCAT‑ACGTCTCAACTTGCTTctg                                        >  1:3220116/1‑150 (MQ=255)
                                                                                                              ggTGGCAAAGAGGGAACGGAAGGAAGGGGAAAATAATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTct                                         <  1:1765215/150‑1 (MQ=255)
                                                                                                               gTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTctg                                        <  1:224770/150‑1 (MQ=255)
                                                                                                               gTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTctg                                        >  1:2504345/1‑150 (MQ=255)
                                                                                                                  caaaaGATGGAACAGGAGAAAGGGGAAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTctggcc                                     <  2:3234855/146‑1 (MQ=255)
                                                                                                                   cacaGATGGAACAGCAGGAAGGGGAAAATGTTTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTctggcct                                    <  1:2155975/150‑1 (MQ=255)
                                                                                                                   caaagagGGAACAGCAGGAAGGGAAAAATGTTTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTctggcct                                    <  2:3441904/147‑1 (MQ=255)
                                                                                                                    acaGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTctggcctg                                   >  2:225347/1‑150 (MQ=255)
                                                                                                                     caGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTctggcctgg                                  >  1:233762/1‑150 (MQ=255)
                                                                                                                      agaggaaaCAGAAGGAAGGGAAAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTTCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTctggcctggc                                 <  2:2820608/144‑1 (MQ=255)
                                                                                                                        aTGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCctggctg                               >  2:246425/1‑150 (MQ=255)
                                                                                                                          ggAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTgga                             >  2:1061640/1‑150 (MQ=255)
                                                                                                                             aCAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTggagga                          >  2:103689/1‑150 (MQ=255)
                                                                                                                               agcagGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAGGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGAcc                        >  1:3620707/1‑150 (MQ=255)
                                                                                                                                gcagGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCa                       >  1:567497/1‑150 (MQ=255)
                                                                                                                                  agGAAGGGGAAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCAtt                     <  1:2398834/150‑1 (MQ=255)
                                                                                                                                   ggAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCAttt                    >  2:4004180/1‑150 (MQ=255)
                                                                                                                                     aaGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCTTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTg                  >  1:2289687/1‑150 (MQ=255)
                                                                                                                                     aaGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTg                  >  2:365970/1‑150 (MQ=255)
                                                                                                                                         ggAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGa              >  1:600269/1‑150 (MQ=255)
                                                                                                                                          gAAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGAt             <  2:2862626/150‑1 (MQ=255)
                                                                                                                                          gAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGAt             <  1:763529/150‑1 (MQ=255)
                                                                                                                                          gAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGAt             >  2:1219695/1‑150 (MQ=255)
                                                                                                                                           aaaaTGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATg            >  1:2194896/1‑150 (MQ=255)
                                                                                                                                              aTGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAgg         >  1:1856769/1‑150 (MQ=255)
                                                                                                                                               tgttttCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAGGt        <  2:2533058/147‑1 (MQ=255)
                                                                                                                                                gATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAGGTg       >  1:2024298/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CGATTTCAGTTTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAGGTGAATTG  >  AP012306/3527219‑3527517

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑