Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123063,639,0400TG25.6% 179.8 / 11.0 82noncoding (54/76 nt)gltVtRNA‑Glu
Reads supporting (aligned to +/- strand):  ref base T (1/60);  new base G (21/0);  total (22/60)
Fisher's exact test for biased strand distribution p-value = 1.20e-18
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 151 total aligned reads displayed.

CACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACA‑GGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCTTAA  >  AP012306/3638892‑3639097
                                                                                                                                                     |                                                         
cACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACA‑GGGGtt                                                          <  2:2034030/150‑1 (MQ=32)
 aCCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACA‑GGGGTTc                                                         <  2:3836048/150‑1 (MQ=32)
 aCCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGGAACA‑CGGGGTc                                                         >  1:3722265/1‑150 (MQ=12)
  cctcctTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACA‑AGGGGTCg                                                        >  1:1000628/1‑150 (MQ=17)
  cctcctTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGCAAAA‑CGGGGTCg                                                        >  1:1706609/1‑150 (MQ=255)
   ctcctTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACA‑GGGGTTCGa                                                       >  1:1448363/1‑150 (MQ=32)
   ctcctTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACA‑GGGGGTcca                                                       >  1:2922479/1‑148 (MQ=17)
   ctcctTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACA‑CGGGGTCGa                                                       >  2:2426986/1‑150 (MQ=17)
    tcctTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGCCTGATAGAAAGGGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACA‑TCGGTTCGac                                                      >  2:269355/1‑149 (MQ=255)
     ccttaccttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACA‑GGGGTTCGAAt                                                     <  1:2126919/150‑1 (MQ=32)
     ccttaccttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACA‑GGGGTTCGAAt                                                     <  1:4005041/150‑1 (MQ=32)
      cttaccttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTTACATGGGGtttaata                                                     >  1:3923464/1‑145 (MQ=255)
      cttaccttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAAAA‑AGGGTTTGAATc                                                    >  2:1616719/1‑150 (MQ=12)
       ttaccttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACA‑GGGGGTCGAATcc                                                   >  2:1250331/1‑150 (MQ=17)
       ttaccttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACA‑GGGGGTCGAATcc                                                   >  2:2414982/1‑150 (MQ=17)
        taccttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACA‑GGGGTTCGAATccc                                                  <  2:439944/150‑1 (MQ=32)
        taccttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACA‑GGGGTTCGAATccc                                                  >  1:1859780/1‑150 (MQ=32)
        taccttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACA‑GGGGTTCGAATccc                                                  <  1:2654568/150‑1 (MQ=32)
         accttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACC‑GGGGGTTCAATcccc                                                 >  1:3258116/1‑150 (MQ=255)
         accttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACA‑GGGGTTCGAATcccc                                                 >  1:2110297/1‑150 (MQ=32)
          ccttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACA‑GGGGTTCGAATCCCCt                                                <  2:369030/150‑1 (MQ=32)
           cttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACA‑GGGGTTCGAATCCCCTa                                               <  2:1367476/150‑1 (MQ=32)
           cttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACA‑GGGGGTCGAATccccca                                               >  2:18455/1‑148 (MQ=17)
            ttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACA‑GGGGTTCGAATCCCCTAg                                              <  1:2649839/150‑1 (MQ=32)
            ttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACA‑GGGGTTCGAATCCCCTAg                                              <  2:85091/150‑1 (MQ=32)
            ttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACA‑AGGGGTCGAATccccccc                                              >  2:3899736/1‑147 (MQ=12)
            ttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAAAA‑GGGGGTTGAATCCCCCAg                                              >  2:250270/1‑150 (MQ=255)
             taAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACA‑GGGGGTCGAATCCCCTAgg                                             >  2:118929/1‑150 (MQ=17)
              aaaGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACA‑GGGGGTCGAATCCCCTAggg                                            >  2:110067/1‑150 (MQ=17)
              aaaGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACA‑GGGGGTCGAATCCCCTAggg                                            >  1:2286739/1‑150 (MQ=17)
              aaaGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACA‑GGGGGTCGAATCCCCTAagg                                            >  1:410582/1‑150 (MQ=17)
               aagaagCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAATGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACA‑AGGGTTCGAATCCCCCAgggg                                           >  1:988988/1‑150 (MQ=12)
               aagaagCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACA‑GGGGGTCGAATCCCCCAgggg                                           >  1:699466/1‑150 (MQ=17)
               aagaagCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACA‑AGGGGTCGAAAACCCTAgggg                                           >  1:2522635/1‑150 (MQ=255)
               aagaagCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGGAAAA‑GGGGGTCGAAACCCCCAgggg                                           >  2:3722980/1‑150 (MQ=11)
                agaagCGTTCTTTGGAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACA‑AGGGGTCGGATTCCCCAggggg                                          >  1:2951168/1‑149 (MQ=11)
                agaagCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACA‑GGGGTTCGAATCCCCTAGGGGa                                          <  1:2375565/150‑1 (MQ=32)
                agaagCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACA‑GGGGTTCGAATCCCCTAGGGGa                                          <  1:1976925/150‑1 (MQ=32)
                  aagCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACA‑GGGGTTCGAATCCCCTAGGGGACg                                        <  2:270922/150‑1 (MQ=32)
                  aagCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACA‑AGGGTTTGAATCCCCCAGGGGACg                                        >  2:3183796/1‑150 (MQ=12)
                  aagCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACA‑AGGGGTCGAAACCCCCCGGGGGCg                                        >  1:3156574/1‑150 (MQ=11)
                   agCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACA‑GGGGGTCGAATCCCCTAGGGGACGc                                       >  1:1200018/1‑150 (MQ=17)
                   agCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACA‑GGGGGTCGAATCCCCCAGGGGACGc                                       >  1:1046558/1‑150 (MQ=17)
                    gCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACA‑GGGGTTCGAATCCCCTAGGGGACGcc                                      <  1:2224631/150‑1 (MQ=32)
                    gCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACA‑GGGGGTCGAATCCCCTAGGGGGCGcc                                      >  2:2300507/1‑150 (MQ=17)
                      gTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACA‑GGGGTTCGAATCCCCTAGGGGACGCCAc                                    <  1:209035/150‑1 (MQ=32)
                       ttCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACA‑GGGGGTCGAATCCCCCAGGGGGCGCCCCt                                   >  1:3172074/1‑150 (MQ=255)
                        tCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACA‑GGGGTTCGAATCCCCTAGGGGACGCCACtt                                  <  2:925240/150‑1 (MQ=32)
                        tCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACA‑GGGGTTCGAATCCCCTAGGGGACGCCACtt                                  <  1:3399992/150‑1 (MQ=32)
                         cTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTCATA‑AGGGGTCGAATTCCCTCTGGGACGCCACtta                                 >  1:1655603/1‑149 (MQ=11)
                         cTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACA‑GGGGTTCGAATCCCCTAGGGGACGCCACTTg                                 <  1:510913/150‑1 (MQ=32)
                          tttGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGCAACA‑AGGGTTCGTAACCCCTCGCGGACGCCACATGc                                >  1:228843/1‑150 (MQ=11)
                           ttGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACA‑GGGGGTCGGATCCCCTAGGGGACGCCACCTGCt                               >  1:61811/1‑150 (MQ=12)
                           ttGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACA‑GGGGGTCGAATCCCCCAGGGGGGGCCCCTTGCt                               >  2:3605719/1‑150 (MQ=11)
                             gCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACA‑GGGGTTCGAATCCCCTAGGGGACGCCACTTGCTgg                             <  1:3668004/150‑1 (MQ=32)
                              cAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACA‑GGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGt                            <  1:3433735/150‑1 (MQ=32)
                                gTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACA‑GGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGttt                          <  1:3689268/150‑1 (MQ=32)
                                 tGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACA‑GGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTtg                         <  2:3511075/150‑1 (MQ=32)
                                 tGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACA‑GGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTtg                         <  1:3096403/150‑1 (MQ=32)
                                 tGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACA‑GGGGGTCGCAACCCCTACGGGGCGCCCCCTGCTGGGTtg                         >  1:1073946/1‑150 (MQ=11)
                                 tGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACA‑GGGGGTCGAATCCCCTAGGGGACGCCACTTGCTGGTTtg                         >  1:833686/1‑150 (MQ=17)
                                 tGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACA‑GGGGGTCGAATCCCCCACGGGACGCCCCTTGCTGGTTtg                         >  2:789131/1‑150 (MQ=255)
                                  gCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACA‑CGGGGTCGAAACCCCCCGGGGGCGCCCCTTGCTGGGTtgt                        >  2:43065/1‑150 (MQ=11)
                                   cTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAAGA‑GGGGGTCGAATCCCCCAGGGGGCGCCCCTTGCTGGTTtttg                       >  2:3428865/1‑150 (MQ=11)
                                    tCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACA‑GGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTgtga                      <  2:1619143/150‑1 (MQ=32)
                                    tCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACA‑GGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTgtga                      <  1:838362/150‑1 (MQ=32)
                                    tCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACA‑GGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTgtga                      <  1:2076834/150‑1 (MQ=32)
                                     cacacaGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTTAAA‑GGGGGGCGGAACCCCTAGGGGGAGCCCCTTTCTTTGTTgtgag                     >  2:3337621/1‑150 (MQ=11)
                                     cacacaGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACA‑CGGGGTCGCAGCCCCTAGGGGACGCCACATCCTGGTTTttgag                     >  1:2803234/1‑150 (MQ=11)
                                     cacacaGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAAAA‑GGGGGTCGAATCCCCCAGGGGGCGCCCCCTGCTGGGTTttgag                     >  2:535704/1‑150 (MQ=11)
                                      acacaGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACA‑GGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTgtgagt                    <  1:1587371/150‑1 (MQ=32)
                                      acacaGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACA‑GGGGGTCGAATCCCCTAGGGGACGCCACTTTCTGGGTTgtgagt                    >  1:310297/1‑150 (MQ=12)
                                       cacaGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACA‑GGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTgtgagtg                   <  1:1057512/150‑1 (MQ=32)
                                       cacaGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACA‑GGGGGTCGAATCCCCTAGGGGGCGCCACCTGCTGGGTTgtgagtg                   >  1:3285036/1‑150 (MQ=255)
                                        acaGATTGTCTGATGGAAAGTCAAAAGCAAGGCGTCTTGCGAAGCCGACTTATACGTCCCCTTCGTCTAGAGGCGCAGGACACCGCCCTGTCTCGGCGGTAACC‑GTGGTTCGACTCCCCTAGGGGACGCCACTTGATGGTTTgtgagtga                  <  2:2289262/150‑1 (MQ=11)
                                        acaGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACA‑GGGGGTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTgtgagtga                  >  1:176244/1‑150 (MQ=17)
                                         caGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACA‑GGGGTTCGAATCCCATAGGGGACGCCACTTGCTGGTTTGTGAGTGaa                 <  1:1220866/150‑1 (MQ=17)
                                          aGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGGAAAA‑GGGGGGCGAAACCCCCAGGGGGGGGCACCTGGTGGGGTGTGGGGGaaa                >  2:835945/1‑150 (MQ=11)
                                           gATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACA‑AGGGGTCGAAACCCCCCGGGGACGCCCCCTGCTGGGTTTTGAGTGGAAg               >  2:221024/1‑150 (MQ=11)
                                            aTTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACA‑GGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGt              <  2:2944442/150‑1 (MQ=32)
                                            aTTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACA‑GGGGGTCGAATCCCCTAGGGGACGCCACTTTCTGGGTTGTGAGTGAAAGt              >  1:2740365/1‑150 (MQ=12)
                                            aTTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAAAA‑AGGGGTTGAATCCCCTAGGGGAAGCCCCTTGCTGGGTTGTTGGTGaaaag              >  2:2336230/1‑148 (MQ=11)
                                            aTTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCCAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACA‑GGGGTCCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGt              <  2:3089579/150‑1 (MQ=17)
                                             ttGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACA‑GGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTc             <  1:1587089/150‑1 (MQ=32)
                                             ttGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACA‑GGGGGTCGAATCCCCTCGGGGGCGCCACCTGCTGGGTTTTGTGTGaaaagc             >  2:1788977/1‑147 (MQ=11)
                                              tGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACA‑GGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCa            <  1:1763859/150‑1 (MQ=32)
                                               gTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACA‑GGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCAc           <  1:418693/150‑1 (MQ=32)
                                                tCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACA‑GGGGGTCGAATCCCCTAGGGGGCGCCACTTGCTGGTTTGTGAGTGAAAGTCAcc          >  1:3709591/1‑150 (MQ=17)
                                                 cTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACA‑GGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCt         <  2:2188457/150‑1 (MQ=32)
                                                 cTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACA‑GGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCt         <  2:3903984/150‑1 (MQ=32)
                                                  tGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACA‑GGGGGTCGAATCCCCTAGGGGGCGCCACTTGCTGGTTTTTGAGTGAAAGTCACCTg        >  1:1378724/1‑150 (MQ=12)
                                                   gATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACA‑GGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGc       <  2:1412853/150‑1 (MQ=32)
                                                   gATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACA‑GGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGc       <  1:1852196/150‑1 (MQ=32)
                                                   gATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACA‑GGGGGTCGAATCCCCTCGGGGGCGCCCCTTGCTGGTTTTTGAGTGAAAGACACCTGc       >  2:3307629/1‑150 (MQ=11)
                                                      aGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACA‑GGGGTTCGAATCCCCTAGGGTACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCtt    <  2:3575268/150‑1 (MQ=17)
                                                      aGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACA‑GGGGGTCGAATCCCCTAGGGGGCGCCACTTGCTGGGTTGTGAGTGAAAGTCACCTGCCtt    >  2:2020309/1‑150 (MQ=12)
                                                      aGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACA‑GGGGGTCGAATCCCCCAGGGGGCGCCACTTTCTGGTTTGTGAGTGAAAGTCACCCGCCtt    >  1:2787120/1‑150 (MQ=11)
                                                       gAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACA‑GGGGTTCGAATCCCCTAGGGGACGCCACCTGCTGGTTTGTGAGTGAAAGTCACCTGCCTTa   >  1:307799/1‑150 (MQ=14)
                                                       gAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACA‑AGGGGTCGAATCCCCTAGGGGGCGCCCCTTGCTGGGTTGTGAGGGAAAGGCACCTGCCTTa   >  2:2628389/1‑150 (MQ=11)
                                                       gAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACA‑AGGGGTCGAATCCCCCAGGGGGCGCCCCCTGCTGGGTTGTGGGGGAAAGTTACCTGCCTTa   >  2:341250/1‑150 (MQ=11)
                                                                                                                                                     |                                                         
CACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACA‑GGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCTTAA  >  AP012306/3638892‑3639097

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑