Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123063,903,7910AG22.5% 554.2 / 60.5 227E189G (GAA→GGA) yjgJpredicted transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (24/152);  new base G (51/0);  total (75/152)
Fisher's exact test for biased strand distribution p-value = 1.22e-32
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 325 total aligned reads displayed.

GACCATTTATGACTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGA‑GGAGTGAT‑GCGGGCCTTG‑ACCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAACGTT  >  AP012306/3903642‑3903940
                                                                                                                                                     |                                                                                                                                                         
gACCATTTATGACTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGa                                                                                                                                                           >  2:599976/1‑150 (MQ=255)
    aTTTATGACTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCg                                                                                                                                                       <  1:2666292/150‑1 (MQ=255)
    aTTTATGACTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCg                                                                                                                                                       <  1:2264692/150‑1 (MQ=255)
     tttATGACTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGa                                                                                                                                                      >  1:3550178/1‑150 (MQ=255)
       tATGACTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGGGATa                                                                                                                                                    >  2:3066239/1‑150 (MQ=255)
       tATGACTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATa                                                                                                                                                    <  2:406284/150‑1 (MQ=255)
          gACTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATaaaa                                                                                                                                                 >  1:1761478/1‑150 (MQ=255)
           aCTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATaaaaa                                                                                                                                                >  2:1167388/1‑150 (MQ=255)
             tACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGGGGTaaaaaaa                                                                                                                                              >  2:3812471/1‑148 (MQ=255)
             tACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGGTAAAAAca                                                                                                                                              >  2:633308/1‑149 (MQ=255)
              aCATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGGTaaaaaaca                                                                                                                                             >  2:1701795/1‑147 (MQ=255)
              aCATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTa                                                                                                                                             <  1:2604146/150‑1 (MQ=255)
               cATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAAatat                                                                                                                                            >  2:2073819/1‑150 (MQ=255)
               cATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTAt                                                                                                                                            <  1:2054494/150‑1 (MQ=255)
                    ccAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAACTATTCTTg                                                                                                                                       >  1:756055/1‑150 (MQ=255)
                    ccAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTg                                                                                                                                       <  1:2752759/150‑1 (MQ=255)
                      aGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTga‑g                                                                                                                                    <  1:1813280/150‑1 (MQ=255)
                       ggcggcATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAAATATTCTTgt‑gg                                                                                                                                   >  2:2167374/1‑150 (MQ=255)
                       ggcggcATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTga‑gg                                                                                                                                   <  2:1498299/150‑1 (MQ=255)
                         cggcATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGGGATAAAAAATATTCTTgg‑gggg                                                                                                                                 >  2:1815892/1‑148 (MQ=255)
                          ggcATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATATAAACTATTCTTTA‑GGAGt                                                                                                                                >  2:2456821/1‑150 (MQ=255)
                            cATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGA‑GGAGTGa                                                                                                                              <  1:1576681/150‑1 (MQ=255)
                              tCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAAATATTCTTGA‑GGAGTGAT‑g                                                                                                                           >  1:738999/1‑150 (MQ=255)
                               ccACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACATCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGA‑GGAGTGAT‑Gc                                                                                                                          <  2:2554606/150‑1 (MQ=255)
                                cacaACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGA‑GGAGTGAT‑GCg                                                                                                                         <  1:3768088/150‑1 (MQ=255)
                                  caACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGGTAAAAAATATTCTTGG‑GGGGTGAT‑GCggg                                                                                                                       >  2:2392681/1‑150 (MQ=255)
                                    aCGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGGGATAAAAACTATTCTTGA‑GGAGTGAT‑GAggggc                                                                                                                     >  1:90633/1‑148 (MQ=255)
                                      gcgcACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGA‑GGAGTGAT‑GCGGGCCtt                                                                                                                   <  2:1577694/150‑1 (MQ=255)
                                          acaATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAACTATTCTTGA‑GGAGTGAT‑GCGGGCCTTG‑Acc                                                                                                              >  1:49954/1‑150 (MQ=255)
                                            aaTGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGGGATAAAAAAAATTTTTTA‑GGAGGGAT‑GCGGGGCTTG‑GCCCg                                                                                                            >  2:3234789/1‑150 (MQ=255)
                                            aaTGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAAAAATTTTTTT‑GGAGGGGT‑GCGGGCCTTT‑GCCCg                                                                                                            >  2:2108096/1‑150 (MQ=255)
                                             aTGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAAATATTCTTTA‑GGGGGGAT‑GCGGGGCTTT‑ACCCG‑a                                                                                                          >  2:1094312/1‑150 (MQ=255)
                                               gtgtgACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAAATATTCTTGA‑GGAGTGAT‑GCGGGCCTTG‑ACCCG‑aaa                                                                                                        >  1:1446586/1‑150 (MQ=255)
                                                tgtgACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGGGATAAAAAATATTTTTGA‑GGAGGGGT‑GGGGGCCTTG‑GCCCG‑AAAg                                                                                                       >  2:3489113/1‑150 (MQ=255)
                                                tgtgACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGGTAAAAACTATTCTTGA‑GGAGTGAT‑GCGGGCCTTT‑ACCCG‑aaaa                                                                                                       >  1:3310090/1‑149 (MQ=255)
                                                   gACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGA‑GGAGTGAT‑GCGGGCCTTG‑ACCCG‑AAAGGCg                                                                                                    <  1:3230590/150‑1 (MQ=255)
                                                      tGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGA‑GGAGTGAT‑GCGGGCCTTG‑ACCCG‑AAAGGCGGGa                                                                                                 <  1:1504757/150‑1 (MQ=255)
                                                       gATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTTA‑GGAGGGAT‑GCGGGCCTTG‑ACCCG‑AAAGGCGGGaa                                                                                                >  1:1172309/1‑150 (MQ=255)
                                                       gATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGA‑GGAGTGAT‑GCGGGCCTTG‑ACCCG‑AAAGGCGGGaa                                                                                                <  1:2857539/150‑1 (MQ=255)
                                                        aTTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAAATATTTTTTA‑GGAGTGAT‑GCGGGGCCTG‑ACCCGCAAAGGGGGGaa                                                                                                >  2:1949713/1‑150 (MQ=255)
                                                        aTTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGA‑GGAGTGAT‑GCGGGCCTTG‑ACCCG‑AAAGGCGGGaaa                                                                                               <  2:3208823/150‑1 (MQ=255)
                                                        aTTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGAGGTAAAAAATATTTTTGAGGGGGTAAG‑GGGGGCCTTG‑‑CCCG‑GAAGGCGGGgaa                                                                                               >  2:1595773/1‑150 (MQ=255)
                                                         ttttATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGA‑GGAGTGAT‑GCGGGCCTTG‑ACCCG‑AAAGGCGGGAAAg                                                                                              <  1:2093163/150‑1 (MQ=255)
                                                          tttATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAACTATTCTTTA‑GGAGGGAT‑GCGGGCCTTG‑ACCCC‑GAAGGGGGGGAAgg                                                                                             >  1:3385955/1‑149 (MQ=255)
                                                            tATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGAGATAAAAAATATTCTTTA‑GGGGTGAT‑GCGGGCCTTG‑ACCCG‑AAAGGCGGGGAAacgc                                                                                           >  2:478949/1‑150 (MQ=255)
                                                             aTGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGGGATAAAAAATATTTTTTA‑GGAGTGATGGGGGGCCTTG‑‑CCCG‑AAAAGAGGGGAAacggc                                                                                          >  2:1667272/1‑148 (MQ=255)
                                                             aTGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAACTATTCTTGA‑GGAGTGAT‑GCGGGCCTTG‑ACCCG‑AAAGGCGGGGAAGCGcc                                                                                          >  1:2454644/1‑150 (MQ=255)
                                                              tGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGA‑GGAGTGAT‑GCGGGCCTTG‑ACCCG‑AAAGGCGGGAAAGCGCCt                                                                                         <  1:1040125/150‑1 (MQ=255)
                                                               gAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGA‑GGAGTTAT‑GCGGGCCTTG‑ACCCG‑AAAGGCGGGAAAGCGCCTg                                                                                        <  1:3472653/150‑1 (MQ=255)
                                                                 gTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGA‑GGAGGGAT‑GCGGGGCTTGAACCCA‑AAAGCGGGGAAAgcgctgcc                                                                                       >  1:1135575/1‑146 (MQ=255)
                                                                  tACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGA‑GGAGTGAT‑GCGGGCCTTG‑ACCCG‑AAAGGCGGGAAAGCGCCTGCCg                                                                                     <  2:725449/150‑1 (MQ=255)
                                                                      gtgtTGTTTGGGCTTTCGGGGAAGGCACGAGAGGGGCACACTATAGAACAGCTCTGTGCAACAGCTGCAAGGGCGGGGGAAGGGAAAAAATTTTTTTTTGG‑GGAGTGAT‑GCGGGCCTTG‑CCCGA‑AAAGGCGGAAAAGCGCCTGCCGATaa                                                                                 <  2:3540572/150‑1 (MQ=255)
                                                                         aTGTTTGAGCTTGCTGCGAGGCCGCGAGAGGGGCACTCTATAGATCAACATTGAGCACGATCTGTAAGGGCGGGGCATGGGATAAAAATTAGCTTGGA‑GGAGGGAG‑GGGGGCGTGG‑ACCCG‑AAAGGCGGGAAAGGGCCTGCTGATAAGtt                                                                              <  2:1890681/150‑1 (MQ=255)
                                                                         aTGTCCGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGA‑GGAGTGAT‑GCGGGCCTTG‑ACCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGtt                                                                              <  2:2545434/150‑1 (MQ=255)
                                                                           gTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAAATATTTTTGA‑GGAGTGAT‑GCGGGCCTTG‑ACCCG‑AAAAGCGGGGAAACGCCCGCCGATAAGTtgt                                                                            >  2:3940840/1‑150 (MQ=255)
                                                                             cTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGA‑GGAGTGAT‑GCGGGCCTTG‑ACCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGa                                                                          <  1:3984081/150‑1 (MQ=255)
                                                                              tGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGA‑GGAGTGAT‑GCGGGCCTTG‑ACCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGAt                                                                         <  1:3116450/150‑1 (MQ=255)
                                                                                   tttCGGCGATGGCAGGAGGGGGGCACCCTAAAGAACACCTCGCTGCAGGAGCTGCAAAGGCGGGGGATGCTATAAAACCTATGTTTGG‑GGGTTGGC‑GGGGGCCTTC‑CCCGG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGAc                                                                    <  2:930436/150‑1 (MQ=255)
                                                                                    ttCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGA‑GGAGTGAT‑GCGGGCCTTG‑ACCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACa                                                                   <  1:1705153/150‑1 (MQ=255)
                                                                                         cGAAGGCAGGAGAGGGGCACTCTATAGAAAAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAACCTATTCTTGA‑GGAGTGAT‑GCGGGCCTTG‑ACCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACaataat                                                              <  2:1253894/150‑1 (MQ=255)
                                                                                          gAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAAATATTCTTGT‑GGGGGGGT‑GCGGGCCTTG‑ACCCG‑AAAGGCGGGAAAACGCCCGGCGGGAAAATGTGGTAAGACaaaaaat                                                             >  2:359878/1‑148 (MQ=255)
                                                                                          gAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGA‑GGAGTGAT‑GCGGGCCTTG‑ACCCC‑AAAAGCGGGGAAACGCCCGCCGGTAAGGTGTGGTAAGACaataaat                                                             >  1:1679377/1‑148 (MQ=255)
                                                                                           aaGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGA‑GGAGTGAT‑GCGGGCCTTG‑ACCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTc                                                            <  1:3710952/150‑1 (MQ=255)
                                                                                            aGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGA‑GGAGTGAT‑GCGGGCCTTG‑ACCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCa                                                           <  1:3155953/150‑1 (MQ=255)
                                                                                              gCACGGGAGGGGCACTCTATAGAACACCTCTGTCCAACAGCTGCAATGGCGGGGAAAGCGATAAAAACTATTCTTGA‑GGAGTGAT‑GCGGGCCTTG‑ACCCA‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCAcg                                                         <  2:2268099/150‑1 (MQ=255)
                                                                                                aCGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAAATATTCTTGA‑GGGGGGAT‑GCGGGCCTTG‑ACCCG‑AAAGGCGGGGAAACGCCCGCCGGTAAGTTGTGATAAGACAAAAAATTAcgcg                                                       >  2:2305308/1‑150 (MQ=255)
                                                                                                 cGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGA‑GGAGTGAT‑GCGGGCCTTG‑ACCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGt                                                      <  2:280280/150‑1 (MQ=255)
                                                                                                   agagGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGA‑GGAGTGAT‑GCGGGCCTTG‑ACCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTa                                                    <  2:3006899/150‑1 (MQ=255)
                                                                                                   agagGGGCACTCTATAGAACAACTCTGTGCAACAGCTGAAAGGGCGGGGGAAGCGAAAAAAACTATTCTTGA‑GGAGTGAT‑GCGGGCTTTG‑ACCCG‑AAGGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTa                                                    <  2:2957526/150‑1 (MQ=255)
                                                                                                    gagGGGCACTCTATAGAACACCTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGA‑GGAGTGAT‑GCGGGCCTTG‑ACCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTaa                                                   <  2:822983/150‑1 (MQ=255)
                                                                                                      ggggCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGA‑GGAGTGAT‑GCGGGCCTTG‑ACCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAgg                                                 <  1:818125/150‑1 (MQ=255)
                                                                                                        ggCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGA‑GGAGTGAT‑GCGGGCCTTG‑ACCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCt                                               <  2:3708168/150‑1 (MQ=255)
                                                                                                         gCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGA‑GGAGTGAT‑GCGGGCCTTG‑ACCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTa                                              <  2:2673518/150‑1 (MQ=255)
                                                                                                          cACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGA‑GGAGTGAT‑GCGGGCCTTG‑ACCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAg                                             <  1:2531393/150‑1 (MQ=255)
                                                                                                           aCTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGA‑GGAGTGAT‑GCGGGCCTTG‑ACCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGc                                            <  1:2310941/150‑1 (MQ=255)
                                                                                                              ctATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAAGGATAAAAAATATTCTTTG‑GGGGTGAT‑GCGGGCCTTG‑AACCC‑GAAGGCGGGGAAGCGCCCGCCGCCAAGTTGTGATAAAACAAAAAATAACACCTTTAGGCTAACggg                                         >  2:913190/1‑149 (MQ=255)
                                                                                                                ataGAACAACTCTGTGCAACAGCTGCAAGGGCGGGGGAAGCGATAAAAACTATTCTTGA‑GGAGTGAT‑GCGGGCCTTG‑ACCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAAt                                       <  2:3849236/150‑1 (MQ=255)
                                                                                                                    aacaacTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAAAAATTTTTGG‑GGAGGGAT‑GCGGGCCTTG‑ACCCG‑AAAGGCGGGGAAACGCCCGCCGGTAAGTTGTGGTAAGACAAAAAATTACCCGTTAAGGCTAGCgggaatggt                                   >  2:1396829/1‑143 (MQ=255)
                                                                                                                       aacTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAAATATTCTTGT‑GGGGGGAT‑GCGGGGCTTG‑ACCCG‑AAAAGCGGGGAAAGGCCCGCCGGTAAGATGTGGTAAGACAAAAAATTACGCCGTAAGGGTAGGGGGATTTAtttt                                >  2:2698705/1‑148 (MQ=255)
                                                                                                                        acTCTGTGCAACAGCTGCAATGGCGGGGGGAGGGATAAAAAATATTCTTTT‑GGAGGGAT‑GCGGGCCCTG‑GCCCG‑AAAGGCGGGGAAAAGCCCGCCGGAAAATTTTGATAAAAAAAAAAATTAAGCGGTAAAGGTAGCGGGAATGATTAtt                               >  2:3314449/1‑149 (MQ=255)
                                                                                                                            tgtgCAACAGCTGCAATGGCGGGGGAGGGGAAAAAAACTATTATTGC‑GGAGTGAT‑ACCGGCCTTT‑CGCCG‑AAAAGCGGGTAAGCGCCCGCCGGTAAGCTCAGGCAAAACAAAAATTTAACCGGTAAGGCTTGCGGCATTTAatattcttt                           >  2:2209132/1‑145 (MQ=255)
                                                                                                                             gtgCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGA‑GGAGTGAT‑GCGGGCCTTG‑ACCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTc                          <  1:97699/150‑1 (MQ=255)
                                                                                                                              tgCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGA‑GGAGTGAT‑GCGGGCCTTG‑ACCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCg                         <  1:2810443/150‑1 (MQ=255)
                                                                                                                               gCAACAGCTGCAATGGCGGGGGGAGGGGTAAAAAATATTTTTGG‑GGGGGGAT‑TCGGGGCTTT‑ACCCG‑GAAAGGGGGAAAACGCCTGCCGGTAAAATTTGATAAAAAAAAAAATTACGCGTTAAGGGTAGGGGAATTTATTAttttttttt                        >  2:1019716/1‑147 (MQ=255)
                                                                                                                                cAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGA‑GGAGTGAT‑GCGGGCCTTG‑ACCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGta                       <  2:1782641/150‑1 (MQ=255)
                                                                                                                                 aaCAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGA‑GGAGTGAT‑GCGGGCCTTG‑ACCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGtat                      <  1:3671995/150‑1 (MQ=255)
                                                                                                                                 aaCAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGA‑GGAGTGAT‑GCGGGCCTTG‑ACCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGtat                      <  1:2234621/150‑1 (MQ=255)
                                                                                                                                   cAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGA‑GGAGTGAT‑GCGGGCCTTG‑ACCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATaa                    <  1:2379465/150‑1 (MQ=255)
                                                                                                                                    aGCTGCAATGGGGGGGGAAGCGATAAAAACTATTCTTGA‑GGAGTGAT‑GCGGGCCTTG‑ACCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAAc                   <  2:76381/150‑1 (MQ=255)
                                                                                                                                       tGCAATGGGGGGGGAAGCGATAAAAACTATTCTTGA‑GGAGTGAT‑GCGGGCCTTG‑ACCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGAt                <  2:499121/150‑1 (MQ=255)
                                                                                                                                        gCAATGGCGGGTGAAGCGATAAAAACTATTCTTGA‑GGAGTGAT‑GCGGGCCTTG‑ACCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATa               <  1:124960/150‑1 (MQ=255)
                                                                                                                                          aaTGGCGGGGGAAGCGATAAAAACTATTCTTGA‑GGAGTGAT‑GCGGGCCTTG‑ACCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGa             <  1:2906102/150‑1 (MQ=255)
                                                                                                                                             ggCGGGGGAAGCGATAAAAACTATTCTTGA‑GGAGTGAT‑GCGGGCCTTG‑ACCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAAt          >  2:2887764/1‑150 (MQ=255)
                                                                                                                                             ggCGGGGGAAGCGATAAAAACTATTCTTGA‑GGAGTGAT‑GCGGGCCTTG‑ACCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAAt          <  1:2970889/150‑1 (MQ=255)
                                                                                                                                              gCGGGGGAAGCGATAAAAATTATTCTTGA‑GGAGGGAG‑GGGGGCCTTG‑CCCCG‑AAAGGCGGGAAAGCCCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATg         <  2:852395/150‑1 (MQ=255)
                                                                                                                                                 ggggAGGCGATAAAAACTATTCTTGA‑GGAGTGAG‑GCGGGCCTTG‑ACCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGaaa      <  2:3723226/150‑1 (MQ=255)
                                                                                                                                                 ggggAAGCGATAAAAACTATTCTTGA‑GGAGTGAT‑GCGGGCCTTG‑ACCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGaaa      <  1:2139276/150‑1 (MQ=255)
                                                                                                                                                 ggggAAGCGATAAAAACTATTCTTGA‑GGAGTGAT‑GCGGGCCTTG‑ACCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGaaa      <  1:775621/150‑1 (MQ=255)
                                                                                                                                                   ggAAGCGATAAAAACTATTCTTGA‑GGAGTGAT‑GCGGGCTTTG‑ACCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAACg    <  1:1537230/150‑1 (MQ=255)
                                                                                                                                                    aaaGCAATAAAAACTATTCTTGA‑GGGGTGAT‑GCGGGCCTGG‑ACCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAACgt   <  2:3650634/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                         
GACCATTTATGACTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGA‑GGAGTGAT‑GCGGGCCTTG‑ACCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAACGTT  >  AP012306/3903642‑3903940

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑