Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123063,938,8870CT33.0% 313.2 / 142.5 188S44F (TCT→TTT) bglJDNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (11/115);  new base T (62/0);  total (73/115)
Fisher's exact test for biased strand distribution p-value = 9.85e-39
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 240 total aligned reads displayed.

ACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTG‑CCTGACTG‑AACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACG  >  AP012306/3938738‑3939036
                                                                                                                                                     |                                                                                                                                                        
aCTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttc                                                                                                                                                          <  2:1337049/150‑1 (MQ=255)
  tCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttttt                                                                                                                                                        >  1:3415788/1‑150 (MQ=255)
  tCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttctt                                                                                                                                                        <  1:1093873/150‑1 (MQ=255)
  tCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttctt                                                                                                                                                        <  2:1648125/150‑1 (MQ=255)
    aTAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttttttt                                                                                                                                                      >  2:392867/1‑150 (MQ=255)
     tAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCGtttttttttttt                                                                                                                                                     >  2:3794070/1‑150 (MQ=255)
     tAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttcttttt                                                                                                                                                     <  1:1000997/150‑1 (MQ=255)
     tAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttcttttt                                                                                                                                                     <  2:1861931/150‑1 (MQ=255)
     tAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttcttttt                                                                                                                                                     >  1:1480527/1‑150 (MQ=255)
      aGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttctttttc                                                                                                                                                    <  2:1076965/150‑1 (MQ=255)
      aGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttctttttc                                                                                                                                                    <  1:2317880/150‑1 (MQ=255)
       gAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATGCCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTttttctttttct                                                                                                                                                   <  2:724571/150‑1 (MQ=255)
        aaaaaTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTg                                                                                                                                                  <  2:222178/150‑1 (MQ=255)
        aaaaaTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTg                                                                                                                                                  <  1:2928914/150‑1 (MQ=255)
             tGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATg                                                                                                                                             <  2:1017079/150‑1 (MQ=255)
              gCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATga                                                                                                                                            <  1:999536/150‑1 (MQ=255)
              gCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATga                                                                                                                                            <  1:3404189/150‑1 (MQ=255)
                 tCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGaa                                                                                                                                         <  1:1713314/150‑1 (MQ=255)
                  cATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTTAGaaa                                                                                                                                        >  2:2158247/1‑149 (MQ=255)
                   aTGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCTTTAGAAGt                                                                                                                                       >  2:536751/1‑150 (MQ=255)
                       gtagtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTCTTTTTTTTCCCTGAGAAGTGAgg                                                                                                                                   >  1:3131207/1‑149 (MQ=255)
                       gtagtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAgc                                                                                                                                   <  1:1316690/150‑1 (MQ=255)
                        tagtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAAGTGAgcg                                                                                                                                  >  1:1597889/1‑150 (MQ=255)
                         agtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTTCATGAGAAGGGAgggc                                                                                                                                 >  2:3047351/1‑150 (MQ=255)
                          gtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCa                                                                                                                                <  1:3046486/150‑1 (MQ=255)
                           taTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTTTTTTTTTTTTTTTTTTCCATGAGAAGTGAggggag                                                                                                                               >  2:2420255/1‑145 (MQ=255)
                            aTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGCGAAGTGAGGGCaga                                                                                                                              >  1:1624588/1‑150 (MQ=255)
                              cGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGGAGTGAGCGCagaga                                                                                                                            >  1:3849146/1‑150 (MQ=255)
                              cGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGAGAGGGCagagg                                                                                                                            >  2:2615483/1‑149 (MQ=255)
                               ggTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAAGTGAGCGCagagag                                                                                                                           >  1:84927/1‑150 (MQ=255)
                               ggTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGTGAGCGCagagag                                                                                                                           >  1:3275616/1‑150 (MQ=255)
                                gTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATTAGAAGAGAGGGGAGAGAgg                                                                                                                          >  2:3672408/1‑150 (MQ=255)
                                gTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAgg                                                                                                                          <  1:213376/150‑1 (MQ=255)
                                   ttGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGAt                                                                                                                       <  2:1994793/150‑1 (MQ=255)
                                     gagaGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTa                                                                                                                     <  1:2541715/150‑1 (MQ=255)
                                      agagTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTAt                                                                                                                    <  2:3426534/150‑1 (MQ=255)
                                         gTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAAGTGAGCGCAGAGGGGGGGTATCtt                                                                                                                 >  1:101500/1‑150 (MQ=255)
                                          tttATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTg                                                                                                                <  2:2058106/150‑1 (MQ=255)
                                           ttATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTG‑c                                                                                                              <  2:1178546/150‑1 (MQ=255)
                                             aTTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTG‑Cct                                                                                                            <  2:2683701/150‑1 (MQ=255)
                                                cAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATTAGAAGGGAGGGCCGGGGGGCGTTATCTTG‑Tcttac                                                                                                         >  2:3294046/1‑150 (MQ=255)
                                                  gAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTG‑Cctgactg                                                                                                       <  1:1288363/150‑1 (MQ=255)
                                                  gAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTG‑Cctgactg                                                                                                       <  1:2689214/150‑1 (MQ=255)
                                                           cGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATTGGGAGGGGGGGCCGGGGGGGGTTATTTTT‑CCTTACTG‑AACttggga                                                                                             >  2:419494/1‑145 (MQ=255)
                                                             ggTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGTCCTGAGAAGAGAGGGCAGAGAGGGGGTATCTTT‑CCTTGCTG‑AAAAGGC‑GCtt                                                                                          >  2:1615465/1‑150 (MQ=255)
                                                              gTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAAGTGAGCGCAGAGGGGGGGTATCTTT‑CCTGACTG‑AACTGGC‑GGttt                                                                                         >  2:3463940/1‑149 (MQ=255)
                                                              gTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTG‑CCTGACTG‑AACTGGC‑GATTa                                                                                         <  2:1400912/150‑1 (MQ=255)
                                                               tAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTG‑CCTGACTG‑AACTGGC‑GATTaa                                                                                        <  1:1815287/150‑1 (MQ=255)
                                                                aaCCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCTTTTTTTTTTTTTTTGCCATTGGAAGGGAGCGGAGGGGGGGATTTTTTTGTCTTGATTG‑‑ACTGGC‑GATTTAg                                                                                       >  2:3115242/1‑150 (MQ=255)
                                                                          aGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTACCTTG‑CCTGACTG‑AACTGGC‑GATTAAGTTTCCGCGTa                                                                             <  1:3473750/150‑1 (MQ=255)
                                                                            cTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCCTTTTTTTTTTTTTTTCACGAGGAGTGAGGGGAGAGAGGGGTTATTTTT‑TCCCACTG‑AACCCGC‑GAGTTAGTTTTCGCGTAcc                                                                           >  2:2349036/1‑150 (MQ=255)
                                                                              ccATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTG‑CCTGACTG‑AACTGGC‑GATTAAGTTTCCGCGTACCCg                                                                         <  1:2007395/150‑1 (MQ=255)
                                                                              ccATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTG‑CCTGACTG‑AACTGGC‑GATTAAGTTTCCGCGTACCCg                                                                         <  1:1120780/150‑1 (MQ=255)
                                                                              ccATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTG‑CCTGACTG‑AACTGGC‑GATTAAGTTTCCGCGTACCCg                                                                         <  1:3497768/150‑1 (MQ=255)
                                                                               cATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTG‑CCTGACTG‑AACTGGC‑GATTAAGTTTCCGCGTACCCgg                                                                        <  1:882744/150‑1 (MQ=255)
                                                                               cATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTG‑CCTGACTG‑AACTGGC‑GATTAAGTTTCCGCGTACCCgg                                                                        <  1:2507282/150‑1 (MQ=255)
                                                                                   ccTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTG‑CCTGACTG‑AACTGGC‑GATTAAGTTTCCGCGTACCCGGCGtt                                                                    <  1:2045324/150‑1 (MQ=255)
                                                                                    cTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTG‑CCTGACTG‑AACTGGC‑GATTAAGTTTCCGCGTACCCGGCGttt                                                                   <  1:3218985/150‑1 (MQ=255)
                                                                                        aCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTG‑CCTGACTG‑AACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGtt                                                               <  2:3892392/150‑1 (MQ=255)
                                                                                          cAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATTAGAAGAGGGGGGAGAGAGGGGATTTCTTT‑CCCTGCTGAAACAGCG‑GTTTAAGTTT‑CGCGTACCCCGCGgtttggttt                                                             >  2:1922166/1‑144 (MQ=255)
                                                                                          cAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTG‑CCTGACTG‑AACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTAt                                                             <  2:621337/150‑1 (MQ=255)
                                                                                           aGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTG‑CCTGACTG‑AACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTAtt                                                            <  1:440742/150‑1 (MQ=255)
                                                                                            gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTTCCTGAGAAAGGGGCGGAGGGAGGGGTTATTTTT‑CCTGACTG‑AACCGGCGGGTTAATTTTCCCCG‑ACCCCGCGGTTTGTttttt                                                           >  2:182642/1‑149 (MQ=255)
                                                                                             tCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTG‑CCTGACTG‑AACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGc                                                          <  2:3638419/150‑1 (MQ=255)
                                                                                                 gAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGCATTTTTTTTTTTTTTTCCATGAGAAGGGAGGGCAGAGGGGGGGTATTTTT‑CCTGACTG‑AACTGGG‑GATTAAGTTTTCGCGTGCCCCGCGGTTTGTTTTTTCgggt                                                      >  2:2857751/1‑148 (MQ=255)
                                                                                                     cATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTG‑CCTGACTG‑AACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgatgatg                                                  <  1:558724/150‑1 (MQ=255)
                                                                                                      aTTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAAATGAGCGCAGAGAGGGATTATCTTT‑CCTGACTG‑AACTGGC‑GATTAAATTTTCGCGTACCCCGCGTTTAGTTATTTCGgatgatga                                                 >  2:3095855/1‑150 (MQ=255)
                                                                                                        ttCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTG‑CCTGACTG‑AACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGata                                               <  2:1598821/150‑1 (MQ=255)
                                                                                                         tCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTG‑CCTGACTG‑AACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGatat                                              <  2:2276239/150‑1 (MQ=255)
                                                                                                         tCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTG‑CCTGACTG‑AACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGatat                                              <  1:2859623/150‑1 (MQ=255)
                                                                                                           aGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTG‑CCTGACTG‑AACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTg                                            <  1:733894/150‑1 (MQ=255)
                                                                                                            ggATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAAGGGAGCGGAGAGAGGGGGTATCTTT‑CCTGACTG‑AAATGGC‑GGTTTAATTTTCCCGTACCCCGCGGTTTGTTTTTTTGGGTGATGAGAttta                                           >  2:1453796/1‑148 (MQ=255)
                                                                                                             gATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTG‑CCTGACTG‑AACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGaa                                          <  1:3296815/150‑1 (MQ=255)
                                                                                                               tGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTG‑CCTGACTG‑AACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGc                                        <  2:347865/150‑1 (MQ=255)
                                                                                                                gCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTG‑CCTGACTG‑AACTGGC‑GATTAAGTTTCCGTGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCt                                       <  1:1951469/150‑1 (MQ=255)
                                                                                                                  cATGTCGCGGATCTCGTTTGCGTCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTG‑CCTGACTG‑AACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCg                                     <  2:1781727/150‑1 (MQ=255)
                                                                                                                     gTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTG‑CCTGACTG‑AACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCt                                  <  1:3874408/150‑1 (MQ=255)
                                                                                                                      tCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTG‑CCTGACTG‑AACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTg                                 <  1:2558366/150‑1 (MQ=255)
                                                                                                                        gcgGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTTCCATGTGAAATGAGCGCAGAGGGGGGGTATTTTT‑CCTGGCTG‑AAATGGC‑GGTTTAATTTTCGCGTACCCGGCGGTTTGTTATTGCGGGTGATGATATTTAAAATCGGCTGAt                               >  2:3066894/1‑150 (MQ=255)
                                                                                                                        gcgGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTG‑CCTGACTG‑AACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGAt                               <  1:3535336/150‑1 (MQ=255)
                                                                                                                                  tttGCGGCGGTCATTTTTTTTTTTTTTTCCCTTAGAAGAGAGGGCCGGGAGGGGGTATTTTT‑CCTTACTG‑AACTTGC‑GGTTAAGTTTTCGCGTACCCGGCGGTTTGTTATTTCGGGTGATGATATTTAAACTCGGCTGGTTTGTTCAttt                     >  2:3257138/1‑149 (MQ=255)
                                                                                                                                   ttGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTG‑CCTGACTG‑AACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGt                    <  1:3930401/150‑1 (MQ=255)
                                                                                                                                       ggcggTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTG‑CCTGACTG‑AACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGcc                >  1:1408442/1‑150 (MQ=255)
                                                                                                                                         cggTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTG‑CCTGACTG‑AACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCg                                     >  1:1240393/1‑127 (MQ=255)
                                                                                                                                          ggTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTG‑CCTGACTG‑AACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATc             >  1:219144/1‑150 (MQ=255)
                                                                                                                                           gTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTG‑CCTGACTG‑AACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCa            <  1:2396537/150‑1 (MQ=255)
                                                                                                                                            tCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTG‑CCTGACTG‑AACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCAc           >  2:3277280/1‑150 (MQ=255)
                                                                                                                                             cATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTG‑CCTGACTG‑AACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCAcc          <  1:2253215/150‑1 (MQ=255)
                                                                                                                                             cATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTG‑CCTGACTG‑AACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCAcc          <  2:28288/150‑1 (MQ=255)
                                                                                                                                              aTTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTG‑CCTGACTG‑AACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCg         <  2:2937700/150‑1 (MQ=255)
                                                                                                                                                  tttctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTG‑CCTGACTG‑AACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTgg     >  2:3808152/1‑150 (MQ=255)
                                                                                                                                                   ttctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTG‑CCTGACTG‑AACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGa    >  2:1098061/1‑150 (MQ=255)
                                                                                                                                                   ttctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTG‑CCTGACTG‑AACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGa    <  2:460460/150‑1 (MQ=255)
                                                                                                                                                   ttctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTG‑CCTGACTG‑AACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGa    >  2:2236703/1‑150 (MQ=255)
                                                                                                                                                   ttctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTG‑CCTGACTG‑AACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGa    <  2:2518586/150‑1 (MQ=255)
                                                                                                                                                    tctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTG‑CCTGACTG‑AACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGAc   >  1:2786586/1‑150 (MQ=255)
                                                                                                                                                    tctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTG‑CCTGACTG‑AACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGAc   >  2:480098/1‑150 (MQ=255)
                                                                                                                                                    tctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTG‑CCTGACTG‑AACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGAc   <  1:1187556/150‑1 (MQ=255)
                                                                                                                                                     ctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTG‑CCTGACTG‑AACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACg  >  1:1897310/1‑150 (MQ=255)
                                                                                                                                                     ctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTG‑CCTGACTG‑AACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACg  <  2:2717188/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                        
ACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTG‑CCTGACTG‑AACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACG  >  AP012306/3938738‑3939036

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑