Predicted mutation
evidence seq id position mutation annotation gene description
RA AP012306 610,004 C→T E167K (GAA→AAA)  abrB ← predicted regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP012306610,0040CT98.9% 682.3 / ‑1.6 177E167K (GAA→AAA) abrBpredicted regulator
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base T (63/112);  total (64/113)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.12e-01

Only 100 of 242 total aligned reads displayed.

CTGCGCCCGCCAGCATCGGGATGAGCATCGTGCCGGAAGGAAGACGCAGCAGGCATCCCACTGTACCGGCAACGACCGCGAGCAAAATAGTAAGCAGGAGATTAATGCTCACTGGCGGGAACCAGACGATATGCTGGTTAACCGCTTCAGCGTTATCGCCCAGCATCATGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAG  >  AP012306/609857‑610150
                                                                                                                                                   |                                                                                                                                                  
cggcgcCCGCCAGCATCGGGATGAGCATCGTGCCGGAAGGAAGACGCAGCAGGCATCCCACTGTACCGGCAACGACCGCGAGCAAAATAGTAAGCAGGAGATTAATGCTCACTGGCGGGAACCAGACGATATGCTGGTTAACCGCTTTAg                                                                                                                                                  <  1:2166191/148‑1 (MQ=255)
cTGCGCCCGCCAGCATCGGGATGAGCATCGTGCCGGAAGGAAGACGCAGCAGGCATCCCACTGTACCGGCAACGACCGCGAGCAAAATAGTAAGCAGGAGATTAATGCTCACTGGCGGGAACCAGACGATATGCTGGTTAACCGCTTTAg                                                                                                                                                  <  1:595540/150‑1 (MQ=255)
  gcccccgccAGCATCGGGATGAGCATCGTGCCGGAAGGAAGACGCTGCAGGCATCCCACTGTACCGGCAACGACCGCGAGCAAAATAGTAAGCAGGAGATTAATGCTCACTGGCGGGAACCAGACGATATGCTGGTTAACCGCTTTAGCg                                                                                                                                                <  2:3205667/147‑1 (MQ=255)
   cccccgccAGCATCGGGATGAGCATCGTGCCGGAAGGAAGACGCAGCAGGCATCCCACTGTACCGGCAACGACCGCGAGCAAAATAGTAAGCAGGAGATTAATGCTCACTGGCGGGAACCAGACGATATGCTGGTTAACCGCTTTAGCGt                                                                                                                                               <  2:3409975/148‑1 (MQ=255)
    gcccgccAGCATCGGGATGAGCATCGTGCCGGAAGGAAGACGCAGCAGGCATCCCACTGTACCGGCAACGACCGCGAGCAAAATAGTAAGCAGGAGATTAATGCTCACTGGCGGGAACCAGACGATATGCTGGTTAACCGCTTTAGCGtt                                                                                                                                              <  2:35273/150‑1 (MQ=255)
     cccgccAGCATCGGGATGAGCATCGTGCCGGAAGGAAGACGCAGCAGGCATCCCACTGTACCGGCAACGACCGCGAGCAAAATAGTAAGCAGGAGATTAATGCTCACTGGCGGGAACCAGACGATATGCTGGTTAACCGCTTTAGCGTTa                                                                                                                                             <  1:3108890/150‑1 (MQ=255)
      ccgccAGCATCGGGATGAGCATCGTGCCGGAAGGAAGACGCAGCAGGCATCCCACTGTACCGGCAACGACCGCGAGCAAAATAGTAAGCAGGAGATTAATGCTCACTGGCGGGGACCAAACGATATGCTGGGTAACCGCCTTTGCGTTAt                                                                                                                                            >  2:3810938/1‑150 (MQ=255)
      ccgccAGCATCGGGATGAGCATCGTGCCGGAAGGAAGACGCAGCAGGCATCCCACTGTACCGGCAACGACCGCGAGCAAAATAGTAAGCAGGAGATTAATGCTCACTGGCGGGAACCAGACGATATGCTGGTTAACCGCTTTAGCGTTAt                                                                                                                                            <  1:2673592/150‑1 (MQ=255)
        gccAGCATCGGGATGCGCATCGTGCCGGAAGGAAGACGCAGCAGGCATCCCACTGTACCGGCAACGACCGCGAGCAAAATAGTAAGCAGGAGATTAACGCTCACTGGCGGGAACCAGACGATATGCTGGTTAACCGCTTTAGCGTTATCg                                                                                                                                          <  2:3298953/150‑1 (MQ=255)
           aGCATAGGGATGAGCATCGTGCCGGAAGGAAGACGCAGCAGGCATCCCACTGTACCGGCAACGACCGCGAGCAAAATAGTAAGCAGGAGATTAATGCTCACTGGCGGGGACCCGACGATATGCTGGGTAACCGCTTTAGCGTTTTCGccc                                                                                                                                       >  2:2050695/1‑150 (MQ=255)
             cATCGGGATGAGCATCGTGCCGGAAGGAAGACGCAGCAGGCATCCCACTGTACCGGCAACGACCGCGAGCAAAATAGTAAGCAGGAGATTAATGCTCACTGGCGGGGACCAGACGATAAGCTGGCTAACCGCGTTTGCGTTAttcccccc                                                                                                                                     >  2:2574305/1‑143 (MQ=255)
              aTCGGGATGAGCATCGTGCCGGAAGGAAGACGCAGCAGGCATCCCACTGTACCGGCAACGACCGCGAGCAAAATAGTAAGCAGGAGATTAATGCTCACTGGCGGGAACCAGACGATATGCTGGTTAACCGCTTTAGCGTTATCGCCCAGc                                                                                                                                    <  1:3666841/150‑1 (MQ=255)
               tCGGGATGAGCATCGTGCCGGAAGGAAGACGCAGCAGGCATCCCACTGTACCGGCAACGACCGCGAGCAAAATAGTAAGCAGGAGATTAATGCTCACTGGCGGGAACCAGACGATATGCTGGTTAACCGCTTTAGCGTTATCGCCCAGca                                                                                                                                   <  1:757511/150‑1 (MQ=255)
                 gggATGAGCATCGTGCCGGAAGGAAGACGCAGCAGGCATCCCACTGTACCGGCAACGACCGCGAGCAAAATAGTAAGCAGGAGATTAATGCTCACTGGCGGGAACCAGACGAGATGCTGGTTAACCACGTTATCGTTGTTGCCCAGccaa                                                                                                                                 >  2:3610383/1‑147 (MQ=255)
                  ggATGAGCATCGTGCCGGAAGGAAGACGCAGCAGGCATCCCACTGTACCGGCAACGACCGCGAGCAAAATAGTAAGCAGGAGATTAATGCTCACTGGCGGGAACCAGACGATATGCTGGTTAACCGCTTTAGCGTTATCGCCCAGcatca                                                                                                                                <  1:207024/150‑1 (MQ=255)
                   gATGAGCATCGTGCCGGAAGGAAGACGCAGCAGGCATCCCACTGTACCGGCAACGACCGCGAGCAAAATAGTAAGCAGGAGATTAATGCTCACTGGCGGGGACCAGACGAGATGGTGGTTAACCGCCTTTGCGGTATCGCCCAGcatcat                                                                                                                               >  2:2285829/1‑150 (MQ=255)
                   gATGAGCATCGTGCCGGAAGGAAGACGCAGCAGGCATCCCACTGTACCGGCAACGACCGCGAGCAAAATAGTAAGCAGGAGATTAATGCTCACTGGCGGGAACCAGACGATATGCTGGTTAACCGCTTTAGCGTTATCGCCCAGcatcat                                                                                                                               <  1:770970/150‑1 (MQ=255)
                     tGAGCATCGTGCCGGAAGGAAGACGCAGCAGGCATCCCACTGTACCGGCAACGACCGCGAGCAAAATAGTAAGCAGGAGATTAATGCTCACTGGCGGGAACCAGACGATATGCTGGTTAACCGCTTTAGCGTTATCGCCCAGCATCATGc                                                                                                                             <  2:2389126/150‑1 (MQ=255)
                            cGTGCCGGAAGGAAGACGCAGCAGGCATCCCACTGTACCGGCAACGACCGCGAGCAAAATAGTAAGCAGGAGATTAATGCTCACTGGCGGGAACCAGACGAAATGCTGGTTAACCGCTTTAGCGTTATCGCCCAGCATCATCCGGGGGAc                                                                                                                      >  1:1048840/1‑150 (MQ=255)
                             gTGCCGGAAGGAAGACGCAGCAGGCATCCCACTGTACCGGCAACGACCGCGAGCAAAATAGTAAGCAGGAGATTAATGCTCACTGGCGGGAACCAGACGATATGCTGGTTAACCGCTTTAGCGTTATCGCCCAGCATCATGCGGGTGAcc                                                                                                                     <  2:240848/150‑1 (MQ=255)
                                 cGGAAGGAAGACGCAGCAGGCATCCCACTGTACCGGCAACGACCGCGAGCAAAATAGTAAGCAGGAGATTAATGCTCACTGGCGGGAAACAGACGATATGCTGGTTAACCCCTTTAGCGTTATCGCCCAGCATCATGCGGGGGACCAGaa                                                                                                                 >  1:3422183/1‑150 (MQ=255)
                                     aggaagACGCAGCAGGCATCCCACTGTACCGGCAACGACCGCGAGCAAAATAGTAAGCAGGAGATTAATGCTCACTGGCGGGGACCCGACGACATAGTGGTTTACCCCCTTTGCGTTATCGCCCCGCATACTGCGGGGGAAGAGaaaaac                                                                                                             >  2:2946812/1‑146 (MQ=255)
                                     aggaagACGCAGCAGGCATCCCACTGTACCGGCAACGACCGCGAGCAAAATAGTAAGCAGGAGATTAATGCTCACTGGCGGGAACCAGACGATATGCTGGTTAACCGCTTTAGCGTTATCGCCCAGCATCATGCGGGTGACCAGAACAGc                                                                                                             <  2:1504429/150‑1 (MQ=255)
                                          gACGCAGCAGGCATCCCACTGTACCGGCAACGACCGCGAGCAAAATAGTAAGCAGGAGATTAATGCTCACTGGCGGGAACCAGACGATATGCTGGTTTAACGCTTTTGCGTTATCGCCCCGCATCATGCGGGGGGCCAGAACAACGGccc                                                                                                        >  2:1357687/1‑150 (MQ=255)
                                           aCGCAGCAGGCATCCCACTGTACCGGCAACGACCGCGAGCAAAATAGTAAGCAGGAGATTAATGCTCACTGGCGGGAACCAGACGATATGCTGGTTAACCGCTTTAGCGTTATCGCCCAGCATCATGCGGGTGACCAGAACAGCGGcccc                                                                                                       <  2:2373069/150‑1 (MQ=255)
                                           aCGCAGCAGGCATCCCACTGTACCGGCAACGACCGCGAGCAAAATAGTAAGCAGGAGATTAATGCTCACTGGCGGGAAACAGGCGATACACTGGTTAAACGCTTTATCGGTATTGCCCCGCCTCAAGTGGGGGTTCAAAACACAGGcccc                                                                                                       >  2:3883430/1‑150 (MQ=255)
                                                 cagGCATCCCACTGTACCGGCAACGACCGCGAGCAAAATAGTAAGCAGGAGATTAATGCTCACTGGCGGGAACCAGACGATATTCTGGTTAACCGCTTTTGCGTTATCGCCCAGCATCATGCGGGGGACCAGAACAGCGGCCCCCCcgac                                                                                                 >  2:3672975/1‑150 (MQ=255)
                                                        cccACTGTACCGGCAACGACCGCGAGCAAAATAGTAAGCAGGAGATTAATGCTCACTGGCGGGAACCAGACGATATGCTGGTTAACCGCTTTAGCGTTATCGCCCAGCATCATGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGc                                                                                          <  2:3082061/150‑1 (MQ=255)
                                                           aCTGTACCGGCAACGACCGCGAGCAAAATAGTAAGCAGGAGATTAATGCTCACTGGCGGGAACCAGACGATATGCTGGTTAACCGCTTTAGCGTTATCGCCCAGCATCATGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACt                                                                                       >  1:2450424/1‑150 (MQ=255)
                                                           aCTGTACCGGCAACGACCGCGAGCAAAATAGTAAGCAGGAGATTAATGCTCACTGGCGGGAACCAGACGATATGCTGGTTAACCGCTTTAGCGTTATCGCCCAGCATCATGCGGGGGACCAGAACAACGGCCCCCCCGACGAACAGCACt                                                                                       >  2:1426/1‑150 (MQ=255)
                                                           aCTGTACCGGCAACGACCGCGAGCAAAATAGTAAGCAGGAGATTAATGCTCACTGGCGGGAACCAGACGATATGCTGGTTAAACGCTTTAGCGTTATCGCCCCGCATCATGCGGGGGACCAGAACAGCGGCCCCCCCGACGAACAGCACt                                                                                       >  2:1780159/1‑150 (MQ=255)
                                                             tGTACCGGCAACGACCGCGAGCAAAATAGTAAGCAGGAGATTAATGCTCACTGGCGGGAACCAGACGATATGCTGGTTAACCGCTTTAGCGTTATCGCCCAGCATCATGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCg                                                                                     <  2:540553/150‑1 (MQ=255)
                                                              gTACCGGCAACGACCGCGAGCAAAATAGTAAGCAGGAGATTAATGCTCACTGGCGGGAACCAGACGATATGCTGGTTAACCGCTTTAGCGTTATCGCCCAGCATCATGCGGGGGACCAGAACAGCGGCCCCCCCGACGAACAGCACCCGc                                                                                    >  1:3043912/1‑150 (MQ=255)
                                                               tACCGGCAACGACCGCGAGCAAAATAGTAAGCAGGAGATTAATGCTCACTGGCGGGAACCAGACGATATGCTGGTTAACCGCTTTAGCGTTATCGCCCAGCATCATGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCa                                                                                   <  2:3054241/150‑1 (MQ=255)
                                                                  cGGCAACGACCGCGAGCAAAATAGTAAGCAGGAGATTAATGCTCACTGGCGGGAACCAGACGATATGCTGGTTAACCGCTTTAGCGTTATCGCCCAGCATCATGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACCGCACTCGCAGat                                                                                <  2:3914861/150‑1 (MQ=255)
                                                                   ggCAACGACCGCGAGCAAAATAGTAAGCAGGAGATTAATGCTCACTGGCGGGAACCAGACGATATGCTGGTTAACCGCTTTAGCGTTATCGCCCAGCATCATGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGata                                                                               <  1:662705/150‑1 (MQ=255)
                                                                    gCAACGACCGCGAGCAAAATAGTAAGCAGGAGATTAATGCTCACTGGCGGGAACCAGACGATATGCTGGTTAACCGCTTTAGCGTTATCGCCCCGCATCATGCGGGGGACCAGAACAACGGCCCCCCCGCCGAACAGCACCCGCAGatat                                                                              >  2:687845/1‑150 (MQ=255)
                                                                    gCAACGACCGCGAGCAAAATAGTAAGCAGGAGATTAATGCTCACTGGCGGGAACCAGACGATATGCTGGTTAAACGCTTTAGCGTTATCGCCCCGCATCATGCGGGGGAACAGAACAGCGGCCCCCCCGACGAACAGCACTCCCAGatat                                                                              >  2:500630/1‑150 (MQ=255)
                                                                    gCAACGACCGCGAGCAAAATAGTAAGCAGGAGATTAATGCTCACTGGCGGGAACCAGACGAAAAGCTGGTTTATCGCGTTAGTGGTATTGCCCAACCTCATGCGGGGGGCCAGAACAGCGGCCCCCGCGGCGAACAGCACTCGCacaaat                                                                              >  2:2808625/1‑145 (MQ=255)
                                                                     cAACGACCGCGAGCAAAATAGTAAGCAGGAGATTAATGCTCACTGGCGGGGAACAAACGATATTCGGGGTAACCCCTTTAGGCTTATAGCCCAGCATCATGCGGGGGGCCAGAACAGCGGGCCCCGCGACGAAAAGCACTCCCAGATAtt                                                                             >  2:1579404/1‑150 (MQ=255)
                                                                      aaCGACCGCGAGCAAAATAGTAAGCAGGAGATTAATGCTCACTGGCGGGGACCCGACGAAATGCTGGGTAACCGCTTTAGCGTTATCGCCCCGCATCATGCGGGGGACCAGAAAAGCGGCCCCCCCCACGAACAGCACTCCCAGATATTg                                                                            >  2:266888/1‑150 (MQ=255)
                                                                       aCGACCGCGAGCAAAATAGTAAGCAGGAGATTAATGCTCACTGGCGGGAACCAGACGATATGCTGGTTAACCGCTTTAGCGTTATCGCCCAGCATCATGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGc                                                                           <  1:1144787/150‑1 (MQ=255)
                                                                        cGACCGCGAGCAAAATAGTAAGCAGGAGATTAATGCTCACTGGCGGGAACCAGACGATATGCTGGTTAACCGCTTTAGCGTTATCGCCCAGCATCATGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCa                                                                          <  1:2617645/150‑1 (MQ=255)
                                                                           ccGCGAGCAAAATAGTAAGCAGGAGATTAATGCTCACTGGCGGGAACCAGACGATATTCTGGTTAACCGCTTTAGCGGTTTCGCCCAGCCGCATGCGGGGGACCAGAAAAGCGGGCCCCGCGACGCACAGCACCCGCAGAAATTGCATaa                                                                       >  2:3319924/1‑150 (MQ=255)
                                                                           ccGCGAGCAAAATAGTAAGCAGGAGATTAATGCTCACTGGCGGGAACCAGACGATATGCTGGTTAACCGCTTTAGCGTTATCGCCCAGCATCATGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATaa                                                                       <  1:3995735/150‑1 (MQ=255)
                                                                            cgcgAGCAAAATAGTAAGCAGGAGATTAATGCTCACTGGCGGGAACCAGACGATATGCTGGTTAACCGCTTTAGCGTTATCGCCCAGCATCATGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATaaa                                                                      <  2:1971683/150‑1 (MQ=255)
                                                                               gAGCAAAATAGTAAGCAGGAGATTAATGCTCACTGGCGGGGACCAGACGATATGCTGGGTAAACGCTTTAGCGGTATCGCCCAGCATCATGCGGGGGACAAGAAAAGCGGCCCCCCCGACGAACAACACTCGCAGATATTGCATAAACGc                                                                   >  2:628972/1‑150 (MQ=255)
                                                                                aGCAAAATAGTAAGCAGGAGATTAATGCTCACTGGCGGGAACCAAACGAAATGCTGGGTAACCGCCTTAGCGTTATTGCCCAGCACCATGCGGGGGACCAGAACAGCGGCCCCCCCGGCGAACAGCCCTCGCCGATATTTCATaaaaacc                                                                  >  2:1209676/1‑146 (MQ=255)
                                                                                   aaaaTAGTAAGCAGGAGATTAATGCTCACTGGCGGGAACCAGACGATATGCTGGTTAACCGCTTTAGCGTTATCGCCCAGCATCATGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATACTGCATAAACGccacc                                                               <  2:114145/150‑1 (MQ=255)
                                                                                      aTAGTAAGCAGGAGATTAATGCTCACTGGCGGGAACCAGACGATATTTTGGTTAACCCCCTTAGCGTTATCGCCCAGCATCATGCGGGTGACCAGAACAACGGCCCCCGCGACGAACAACACTCGCAGATATTTCATAAACGccaccaag                                                            >  2:840624/1‑148 (MQ=255)
                                                                                       tAGTAAGCAGGAGATTAATGCTCACTGGCGGGAACCAGACGATATGCTGGTTAACCGCTTTAGCGTTATCGCCCAGCATCATGCGGGGGACCAGAACAACGGCCCCCCCGCCGAACAGCACTCCCAGATATTGCATAAACGCCACCCGGc                                                           >  2:3332279/1‑150 (MQ=255)
                                                                                         gTAAGCAGGAGATTAATGCTCACTGGCGGGGACCAAACGATATGCTGGTTTACCGCTTTTGCGTTATCCCCCCGCATCATGCGGGGGACCAGAACAGCGGCCCCCCCGCCGAACCGCACTCCCCGATATTGCATAAACGCCACCAGGCgg                                                         >  2:2820535/1‑149 (MQ=255)
                                                                                          tAAGCAGGAGATTAATGCTCACTGGCGGGAACCAGACGATATGCTGGTTAACCGCTTTAGCGTTATCGCCCAGCATCATGCGGGGGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGaa                                                        >  1:2358031/1‑150 (MQ=255)
                                                                                           aaGCAGGAGATTAATGCTCACTGGCGGGAAACAGACGAAATGCTGGGTAAACGCTTTAGCGTTATCGCCCAGCATCCTGCGGGGGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGGCGATATTGCATAAAAGCCCCCAGGCGAat                                                       >  2:1739646/1‑150 (MQ=255)
                                                                                              cAGGAGATTAATGCTCACTGGCGGGAACCAGACGATATGCTGGGTAAACGCTTTAGCGTTATCGCCCAGCATCATGCGGGGGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATc                                                    >  1:464957/1‑150 (MQ=255)
                                                                                              cAGGAGATTAATGCTCACTGGCGGGAACCAGAAGAAAAGCTGGTTAACCGCGTTTGCGTTATCGGCCAGCAACAAGCGGGGGGCCAGAAAAGAGGCCCCCGCGACGGACAGCACACGCAGATATTGGATAAACGCCACCAGGCGGATATc                                                    >  2:349561/1‑150 (MQ=255)
                                                                                               aGGAGATTAATGCTCACTGGCGGGAACCAGACGATATGCTGGTTAACCGCTTTAGCGTTATCGCCCAGCATCATGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCt                                                   <  2:912297/150‑1 (MQ=255)
                                                                                                  agaTTAATGCTCACTGGCGGGGACCAGAAGATATGCTGGTTAACCGCTTTAGCGTTTTCGCCCCGCATCATGCGGGGGACCAGAACAGCGGCCCCCCCGACGAAAAGCACTCGCAGATATTTCATAAACGCCACCAGGCGAATATCTgcg                                                >  2:254862/1‑150 (MQ=255)
                                                                                                  agaTTAATGCTCACTGGCGGGAACCAGACGATATGCTGGTTAACCGCTTTAGCGGTATCGCCCAGCATCCTGCGGGGGACCAGAACAGCGGCCCCCCCGACGAACAGCACCCGCAGATATTGCATAAAAGCCACCAGGCGAAAATCTgcg                                                >  2:1542963/1‑150 (MQ=255)
                                                                                                     ttAATGCTCACTGGCGGGAACCAGACGATATGCTGGTTAACCGCTTTTGCGTTTTTGCCCCGCATCATGCGGGGGACCAGAACAGCGGGCCCCCCGACGAACAGCCCCCGCAGATATTGCATAAACGCCACCAGGCGAATATATGCGCCg                                             >  2:3961298/1‑150 (MQ=255)
                                                                                                       aaTGCTCACTGGCGGGAACCAGACGATATGCTGGTTAACCGCTTTAGCGTTATCGCCCAGCATCATGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTa                                           <  2:2987697/150‑1 (MQ=255)
                                                                                                        aTGCTCACTGGCGGGAACCAGACGATATGCTGGTTAACCGCTTTTGCGTTATCGCCCAGCATCATGCGGGGGACCAGAACAGCGGCCCCCGCGACGAACAGCACCCGCAGATATTGCATAAACGCCACCCGGCGGATATCTGCGCCGGaa                                          >  2:2755856/1‑150 (MQ=255)
                                                                                                        aTGCTCACTGGCGGGAACCAGACGATATGCTGGTTAACCCCTTTAGCGTTATCGCCCAGCATCCTGCGGGGGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCCGGCGAATATCTGCGCCGTaa                                          >  2:2217908/1‑150 (MQ=255)
                                                                                                         tGCTCACTGGCGGGAACCAGACGATATGCTGGTTAACCGCTTTAGCGTTATCGCCCAGCATCATGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAAt                                         <  2:3223918/150‑1 (MQ=255)
                                                                                                          gCTCACTGGCGGGAACCAGACGATATGCTGGTTAACCGCTTTAGCGTTATCGCCCAGCATCATGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATc                                        <  2:4009009/150‑1 (MQ=255)
                                                                                                           cTCACTGGCGGGAACCAGACGATATGCTGGTTAACCGCTTTTGCGTTATCGCCCCGCATCATGCGGGGGACCCGAACAGCGGCCCCCCCCACCAACAGCACCCGCAGAAATTTCATAAACGCCCCCAAGCGAAAATATGCCCCCGAATCt                                       >  2:1613332/1‑150 (MQ=255)
                                                                                                           cTCACTGGCGGGAACCAGACGATATGCTGGTTAACCGCTTTAGCGTTATCGCCCAGCATCATGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCt                                       <  1:1945076/150‑1 (MQ=255)
                                                                                                                tGGCGGGAACCAGACGATATGCTGGTTAACCGCTTTAGCGTTATCGCCCAGCATCATGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTgcgc                                  >  1:1833599/1‑150 (MQ=255)
                                                                                                                tGGCGGGAACCAGACGATATGCTGGTTAACCGCTTCAGCGTTATCGCCCAGCATCATGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTgcgc                                  >  2:3576198/1‑150 (MQ=255)
                                                                                                                 ggCGGGAACCAGACGATATGCTGGTTAACCGCTTTAGCGTTATCGCCCAGCATCATGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGcc                                 >  2:2264575/1‑150 (MQ=255)
                                                                                                                  gCGGGACCCAGACGATATGCTGGTTAACCGCTTTAGCGTTATCGCCCAGCATCATGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCa                                <  1:3623828/150‑1 (MQ=255)
                                                                                                                  gCGGGAACCAGACGATATGCTGGTTAACCGCTTTAGCGTTATCGCCCAGCATCATGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCa                                >  1:1571574/1‑150 (MQ=255)
                                                                                                                  gCGGGAACCAGACGATATGCTGGTTAACCGCTTTAGCGTTATCGCCCAGCATCATGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCa                                <  2:1061675/150‑1 (MQ=255)
                                                                                                                   cGGGAACCAGACGATATGCTGGTTAACCGCTTTAGCGTTATCGCCCAGCATCATGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCAt                               >  1:3864418/1‑150 (MQ=255)
                                                                                                                     ggAACCAGACGATATGCTGGTTAACCGCTTTAGCGTTATCGCCCAGCATCATGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAg                             <  2:3659469/150‑1 (MQ=255)
                                                                                                                      gAACCAGACGATATGCTGGTTAACCGCTTTAGCGTTATCGCCCAGCATCATGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGc                            >  2:3383195/1‑150 (MQ=255)
                                                                                                                        aCCAGACGATATGCTGGTTAACCGCTTTAGCGTTATCGCCCAGCATCATGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGa                          >  2:730706/1‑150 (MQ=255)
                                                                                                                          cAGACGATATGCTGGTTAACCGCTTTAGCGTTATCGCCCAGCATCATGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGAcc                        >  1:983464/1‑150 (MQ=255)
                                                                                                                          cAGACGATATGCTGGTTAACCGCTTTAGCGTTATCGCCCAGCATCATGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGAcc                        >  2:1858634/1‑150 (MQ=255)
                                                                                                                            gACGATATGCTGGTTAACCGCTTTAGCGTTATCGCCCAGCATCATGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCAt                      <  1:2916224/150‑1 (MQ=255)
                                                                                                                             aCGATATGCTGGTTAACCGCTTTAGCGTTATCGCCCAGCATCATGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCAtt                     >  2:656001/1‑150 (MQ=255)
                                                                                                                              cGATATGCTGGTTAACCGCTTTAGCGTTATCGCCCAGCATCATGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTg                    >  1:2397450/1‑150 (MQ=255)
                                                                                                                               gATATGCTGGTTAACCGCTTTAGCGTTATCGCCCAGCATCATGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTgc                   >  2:469509/1‑150 (MQ=255)
                                                                                                                               gATATGCTGGTTAACCGCTTTAGCGTTATCGCCCAGCATCATGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTgc                   >  1:1132834/1‑150 (MQ=255)
                                                                                                                               gATATGCTGGTTAACCGCTTTAGCGTTATCGCCCAGCATCATGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTgc                   >  1:2842708/1‑150 (MQ=255)
                                                                                                                               gATATGCTGGTTAACCGCTTTAGCGTTATCGCCCAGCATCATGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTgc                   <  1:3316595/150‑1 (MQ=255)
                                                                                                                               gATATGCTGGTTAACCGCTTTAGCGTTATCGCCCAGCATCATGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTgc                   <  1:859586/150‑1 (MQ=255)
                                                                                                                               gATATGCTGGTTAACCGCTTTAGCGTTATCGCCCAGCATCATGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTgc                   >  2:2177061/1‑150 (MQ=255)
                                                                                                                                atatGCTGGTTAACCGCTTTAGCGTTATCGCCCAGCATCATGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTgcc                  >  2:3145968/1‑150 (MQ=255)
                                                                                                                                  atGCTGGTTAACCGCTTTAGCGTTATCGCCCAGCATCATGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTgccgc                >  1:374062/1‑150 (MQ=255)
                                                                                                                                  atGCTGGTTAACCGCTTTAGCGTTATCGCCCAGCATCATGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTgccgc                >  1:642646/1‑150 (MQ=255)
                                                                                                                                   tGCTGGTTAACCGCTTTAGCGTTATCGCCCAGCATCATGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTgccgcc               <  2:788600/150‑1 (MQ=255)
                                                                                                                                    gCTGGTTAACCGCTTTAGCGTTATCGCCCAGCATCATGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGccgccg              >  1:2193917/1‑150 (MQ=255)
                                                                                                                                     cTGGTTAACCGCTTTAGCGTTATCGCCCAGCATCATGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCcgccgc             >  1:3766065/1‑150 (MQ=255)
                                                                                                                                      tGGTTAACCGCTTTAGCGTTATCGCCCAGCATCATGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCcgccgcg            >  1:1334799/1‑150 (MQ=255)
                                                                                                                                         ttAACCGCTTTAGCGTTATCGCCCAGCATCATGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCcgccgcgccg         <  2:1072735/150‑1 (MQ=255)
                                                                                                                                          tAACCGCTTTAGCGTTATCGCCCAGCATCATGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCgccgcgccgc        >  2:3827706/1‑150 (MQ=255)
                                                                                                                                           aaCCGCTTTAGCGTTATCGCCCAGCATCATGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCgccgcc       >  2:3437187/1‑150 (MQ=255)
                                                                                                                                              cGCTTTAGCGTTATCGCCCAGCATCATGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCagg    <  1:1780159/150‑1 (MQ=255)
                                                                                                                                              cGCTTTAGCGTTATCGCCCAGCATCATGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCagg    >  2:2614933/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CTGCGCCCGCCAGCATCGGGATGAGCATCGTGCCGGAAGGAAGACGCAGCAGGCATCCCACTGTACCGGCAACGACCGCGAGCAAAATAGTAAGCAGGAGATTAATGCTCACTGGCGGGAACCAGACGATATGCTGGTTAACCGCTTCAGCGTTATCGCCCAGCATCATGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAG  >  AP012306/609857‑610150

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑