Predicted mutation
evidence seq id position mutation annotation gene description
RA AP012306 2,977,561 G→T A172E (GCG→GAG)  nudE ← ADP‑ribose diphosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123062,977,5610GT99.4% 1255.2 / ‑0.1 323A172E (GCG→GAG) nudEADP‑ribose diphosphatase
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base T (226/95);  total (227/96)
Fisher's exact test for biased strand distribution p-value = 5.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.56e-01

Only 100 of 329 total aligned reads displayed.

GCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGTGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCGCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATT  >  AP012306/2977414‑2977707
                                                                                                                                                   |                                                                                                                                                  
 cGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGTGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCTCAc                                                                                                                                                 >  1:1908959/1‑150 (MQ=255)
  gTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGTGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCTCACt                                                                                                                                                <  2:908514/150‑1 (MQ=255)
     aaCGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGTGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCTCACTGAc                                                                                                                                             <  1:3104493/150‑1 (MQ=255)
      aCGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGTGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCTCACTGACa                                                                                                                                            >  1:3466739/1‑150 (MQ=255)
        gcgTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGTGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCTCACTGACAtt                                                                                                                                          >  2:399115/1‑150 (MQ=255)
        gcgTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGTGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCTCACTGACAtt                                                                                                                                          >  2:1754514/1‑150 (MQ=255)
         cgTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGTGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCTCACTGACATTg                                                                                                                                         >  1:2325286/1‑150 (MQ=255)
         cgTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGTGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCTCACTGACATTg                                                                                                                                         >  1:1227933/1‑150 (MQ=255)
          gTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGTGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCTCACTGACATTgc                                                                                                                                        >  1:1752569/1‑150 (MQ=255)
          gTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGTGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCTCACTGACATTgc                                                                                                                                        >  1:2869960/1‑150 (MQ=255)
            tATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGTGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCTCACTGACATTgcgc                                                                                                                                      >  1:1713880/1‑150 (MQ=255)
            tATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGTGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCTCACTGACATTgcgc                                                                                                                                      >  2:1399807/1‑150 (MQ=255)
            tATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGTGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCTCACTGACATTgcgc                                                                                                                                      >  1:850670/1‑150 (MQ=255)
            tATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGTGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCTCACTGACATTgcgc                                                                                                                                      >  2:2339635/1‑150 (MQ=255)
               ccGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGTGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCTCACTGACATTGCGCGCt                                                                                                                                   >  2:1988159/1‑150 (MQ=255)
                cGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGTGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCTCACTGACATTGCGCGCtt                                                                                                                                  <  1:3941135/150‑1 (MQ=255)
                 ggCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGTGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCTCACTGACATTGCGCGCTTc                                                                                                                                 >  2:2108603/1‑150 (MQ=255)
                  gCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGTGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCTCACTGACATTGCGCGCTTCa                                                                                                                                >  1:1382289/1‑150 (MQ=255)
                   cTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGTGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCTCACTGACATTGCGCGCTTCAt                                                                                                                               <  1:284809/150‑1 (MQ=255)
                    tAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGTGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCTCACTGACATTGCGCGCTTCAtt                                                                                                                              >  2:2911965/1‑150 (MQ=255)
                        aaaaTCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGTGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCTCACTGACATTGCGCGCTTCATTGAAg                                                                                                                          >  1:3640324/1‑150 (MQ=255)
                           aTCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGTGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCTCACTGACATTGCGCGCTTCATTGAAGTCa                                                                                                                       >  2:2017211/1‑150 (MQ=255)
                             cGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGTGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCTCACTGACATTGCGCGCTTCATTGAAGTCAgg                                                                                                                     >  2:3887213/1‑150 (MQ=255)
                              gTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGTGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCTCACTGACATTGCGCGCTTCATTGAAGTCAggg                                                                                                                    >  2:524620/1‑150 (MQ=255)
                               tGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGTGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCTCACTGACATTGCGCGCTTCATTGAAGTCAGGGt                                                                                                                   <  1:43554/150‑1 (MQ=255)
                                  aaaCTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGTGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCTCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCtt                                                                                                                <  2:3012265/150‑1 (MQ=255)
                                   aaCTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGTGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCTCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTc                                                                                                               <  2:1791011/150‑1 (MQ=255)
                                       cAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGTGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCTCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGc                                                                                                           >  2:2280612/1‑150 (MQ=255)
                                        aaTAAATTGCAGAAACCCCATAAGCCTGATAAACATTGTGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCTCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCa                                                                                                          >  2:1050660/1‑150 (MQ=255)
                                        aaTAAATTGCAGAAACCCCATAAGCCTGATAAACATTGTGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCTCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCa                                                                                                          >  1:2043383/1‑150 (MQ=255)
                                        aaTAAATTGCAGAAACCCCATAAGCCTGATAAACATTGTGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCTCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCa                                                                                                          >  1:3540318/1‑150 (MQ=255)
                                           aaaTTGCAGAAACCCCATAAGCCTGATAAACATTGTGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCTCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAAt                                                                                                       >  1:15148/1‑150 (MQ=255)
                                            aaTTGCAGAAACCCCATAAGCCTGATAAACATTGTGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCTCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATc                                                                                                      >  1:2100053/1‑150 (MQ=255)
                                            aaTTGCAGAAACCCCATAAGCCTGATAAACATTGTGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCTCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATc                                                                                                      >  2:2690923/1‑150 (MQ=255)
                                              ttGCAGAAACCCCATAAGCCTGATAAACATTGTGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCTCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCca                                                                                                    >  1:3738478/1‑150 (MQ=255)
                                                   gAAACCCCATAAGCCTGATAAACATTGTGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCTCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCata                                                                                               >  2:3248341/1‑150 (MQ=255)
                                                      aCCCCATAAGCCTGATAAACATTGTGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCTCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATgc                                                                                            <  1:970703/150‑1 (MQ=255)
                                                        cccATAAGCCTGATAAACATTGTGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTGAACCATTCGCGCACGAGGAACAGCTCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATgcgc                                                                                          >  2:3035003/1‑150 (MQ=255)
                                                        cccATAAGCCTGATAAACATTGTGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCTCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATgcgc                                                                                          <  1:2638875/150‑1 (MQ=255)
                                                          cATAAGCCTGATAAACATTGTGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCTCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCgcca                                                                                        <  1:3869655/150‑1 (MQ=255)
                                                           aTAAGCCTGATAAACATTGTGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCTCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCgccag                                                                                       >  1:3189648/1‑150 (MQ=255)
                                                                 cTGATAAACATTGTGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCTCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCgccagcggcca                                                                                 >  1:2925799/1‑150 (MQ=255)
                                                                 cTGATAAACATTGTGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCTCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCgccagcggcca                                                                                 >  1:3030870/1‑150 (MQ=255)
                                                                 cTGATAAACATTGTGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCTCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCgccagcggcca                                                                                 >  2:1887046/1‑150 (MQ=255)
                                                                  tGATAAACATTGTGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCTCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCgccagcggccag                                                                                >  2:2680353/1‑150 (MQ=255)
                                                                    aTAAACATTGTGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCTCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCgccagcggccagcg                                                                              >  2:1507365/1‑150 (MQ=255)
                                                                     tAAACATTGTGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCTCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAgcgc                                                                             >  1:1067774/1‑150 (MQ=255)
                                                                        aCATTGTGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCTCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCAcc                                                                          >  1:3301423/1‑150 (MQ=255)
                                                                        aCATTGTGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCTCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCAcc                                                                          >  1:1039801/1‑150 (MQ=255)
                                                                          aTTGTGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCTCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCtg                                                                        >  2:112139/1‑150 (MQ=255)
                                                                             gtgCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCTCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTgg                                                                     >  2:2479740/1‑150 (MQ=255)
                                                                              tgCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCTCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGt                                                                    >  2:93963/1‑150 (MQ=255)
                                                                               gCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCTCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTa                                                                   >  1:2184877/1‑150 (MQ=255)
                                                                               gCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCTCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTa                                                                   <  2:3120214/150‑1 (MQ=255)
                                                                                     ggCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCTCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGct                                                             >  2:1638201/1‑150 (MQ=255)
                                                                                      gCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCTCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGctc                                                            >  1:763212/1‑150 (MQ=255)
                                                                                      gCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCTCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGctc                                                            <  2:2505770/150‑1 (MQ=255)
                                                                                      gCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCTCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGctc                                                            >  1:528955/1‑150 (MQ=255)
                                                                                       cAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCTCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGctct                                                           >  2:3531815/1‑150 (MQ=255)
                                                                                       cAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCACGCACGAGGAACAGCTCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGctct                                                           >  1:2883659/1‑150 (MQ=255)
                                                                                        aaaCTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCTCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTg                                                          >  2:3775604/1‑150 (MQ=255)
                                                                                        aaaCTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCTCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTg                                                          >  1:1816638/1‑150 (MQ=255)
                                                                                          aCTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCTCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGc                                                        <  2:2864768/150‑1 (MQ=255)
                                                                                            ttCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCTCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTc                                                      >  2:76341/1‑150 (MQ=255)
                                                                                              cACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCTCACTGACATTGCGCGCTTCATTGCAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCCCAt                                                    >  2:3444279/1‑150 (MQ=255)
                                                                                              cACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCTCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCAt                                                    >  1:2693966/1‑150 (MQ=255)
                                                                                              cACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCTCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCAt                                                    >  1:934214/1‑150 (MQ=255)
                                                                                                cGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCTCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCg                                                  >  2:1301870/1‑150 (MQ=255)
                                                                                                 gCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCTCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGc                                                 >  2:3931025/1‑150 (MQ=255)
                                                                                                  cATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCTCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGcc                                                >  1:2769294/1‑150 (MQ=255)
                                                                                                  cATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCTCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGcc                                                <  1:2472776/150‑1 (MQ=255)
                                                                                                    tttACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCTCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCtt                                              >  1:1338667/1‑150 (MQ=255)
                                                                                                     ttACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCTCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTc                                             >  2:600927/1‑150 (MQ=255)
                                                                                                     ttACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCTCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTc                                             >  1:1007580/1‑150 (MQ=255)
                                                                                                     ttACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCTCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTc                                             <  2:2769344/150‑1 (MQ=255)
                                                                                                     ttACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCTCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTc                                             <  1:3436299/150‑1 (MQ=255)
                                                                                                     ttACACTCACCCCTGCCCTTTCAACCATTCGCCCACGAGGAACAGCTCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACATGTGGCAGCGGCTCTGGCACATCCCCTTc                                             >  2:1712816/1‑150 (MQ=255)
                                                                                                      tACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCTCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTcc                                            >  1:3227935/1‑150 (MQ=255)
                                                                                                         acTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCTCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGt                                         >  1:553825/1‑150 (MQ=255)
                                                                                                                ccTGCCCTTTCAACCATTCGCGCACGAGGAACAGCTCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCg                                  >  2:2595999/1‑150 (MQ=255)
                                                                                                                  tGCCCTTTCAACCATTCGCGCACGAGGAACAGCTCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCggg                                >  2:3262573/1‑150 (MQ=255)
                                                                                                                   gCCCTTTCAACCATTCGCGCACGAGGAACAGCTCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGt                               <  1:2563336/150‑1 (MQ=255)
                                                                                                                   gCCCTTTCAACCATTCGCGCACGAGGAACAGCTCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGt                               >  1:662951/1‑150 (MQ=255)
                                                                                                                    cccTTTCAACCATTCGCGCACGAGGAACAGCTCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTa                              >  2:1821546/1‑150 (MQ=255)
                                                                                                                     ccTTTCAACCATTCGCGCACGAGGAACAGCTCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTag                             >  1:280080/1‑150 (MQ=255)
                                                                                                                         tCAACCATTCGCGCACGAGGAACAGCTCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGAt                         <  1:2501948/150‑1 (MQ=255)
                                                                                                                         tCAACCATTCGCGCACGAGGAACAGCTCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGAt                         <  1:1588155/150‑1 (MQ=255)
                                                                                                                         tCAACCATTCGCGCACGAGGAACAGCTCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGAt                         <  1:3916373/150‑1 (MQ=255)
                                                                                                                          cAACCATTCGCGCACGAGGAACAGCTCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATc                        >  1:1097552/1‑150 (MQ=255)
                                                                                                                              cATTCGCGCACGAGGAACAGCTCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTgc                    <  2:3354642/150‑1 (MQ=255)
                                                                                                                              cATTCGCGCACGAGGAACAGCTCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTgc                    <  1:1976782/150‑1 (MQ=255)
                                                                                                                               aTTCGCGCACGAGGAACAGCTCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTgcg                   >  1:1620271/1‑150 (MQ=255)
                                                                                                                                       aCGAGGAACAGCTCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACg           >  1:361062/1‑150 (MQ=255)
                                                                                                                                         gAGGAACAGCTCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGat         <  2:799988/150‑1 (MQ=255)
                                                                                                                                             aaCAGCTCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTc     >  2:2712415/1‑150 (MQ=255)
                                                                                                                                             aaCAGCTCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTc     >  2:157675/1‑150 (MQ=255)
                                                                                                                                             aaCAGCTCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTc     >  1:2357424/1‑150 (MQ=255)
                                                                                                                                               cAGCTCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCAt   >  2:3711638/1‑150 (MQ=255)
                                                                                                                                               cAGCTCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCAt   >  2:3404732/1‑150 (MQ=255)
                                                                                                                                                aGCTCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCAtt  <  1:73280/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGTGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCGCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATT  >  AP012306/2977414‑2977707

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑