Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP012306935,0540GT24.0% 346.2 / 59.2 121intergenic (+24/‑1406)putP/ycdOproline:sodium symporter/conserved protein
Reads supporting (aligned to +/- strand):  ref base G (20/72);  new base T (29/0);  total (49/72)
Fisher's exact test for biased strand distribution p-value = 3.84e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 176 total aligned reads displayed.

GGCAGTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCT‑CCCTTA‑ATGTGCCTCGCCATA‑T‑AAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAA‑TCGCCGCCGCCCCCTG‑CT‑G‑TCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGCCACCAACA  >  AP012306/934905‑935203
                                                                                                                                                      |                                                                                                                                                              
ggCAGTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTtttg                                                                                                                                                                <  1:4089031/150‑1 (MQ=255)
   aGTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTtttgttt                                                                                                                                                             <  1:778715/150‑1 (MQ=255)
   aGTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGGttttttt                                                                                                                                                             >  1:2081858/1‑150 (MQ=255)
     tATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACTCTTTGGCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTAGCAGGGAAGCTGAGGGACTTAGCCTGCGTC‑GGCgttgtttgc                                                                                                                                                           >  2:3001178/1‑149 (MQ=255)
     tATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTgg                                                                                                                                                           <  2:3852818/150‑1 (MQ=255)
      aTTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGCtttttttgta                                                                                                                                                          >  2:3989866/1‑148 (MQ=255)
       ttGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCt                                                                                                                                                         <  1:353538/150‑1 (MQ=255)
       ttGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCt                                                                                                                                                         <  1:1830823/150‑1 (MQ=255)
       ttGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGGCTTGTTTTGCt                                                                                                                                                         >  1:1064946/1‑150 (MQ=255)
         gggATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTc                                                                                                                                                       <  1:1508150/150‑1 (MQ=255)
         gggATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGGTTTTTTTTGCTTc                                                                                                                                                       >  1:4221950/1‑150 (MQ=255)
          ggATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTTTTTTGCTTca                                                                                                                                                      >  1:2946472/1‑150 (MQ=255)
          ggATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGCTTTTTTTTGCCTct                                                                                                                                                      >  1:1966003/1‑149 (MQ=255)
           gATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTTTTTTGCTTtag                                                                                                                                                     >  1:2312219/1‑150 (MQ=255)
             ttGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTTGCTTcagca                                                                                                                                                   >  1:1754519/1‑150 (MQ=255)
              tGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTTTTTTGGTTccgctg                                                                                                                                                  >  2:3422602/1‑148 (MQ=255)
                tAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTTTTTGGCTTCCGCAGCg                                                                                                                                                >  1:3880693/1‑150 (MQ=255)
                 aGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTCAGCAGCgg                                                                                                                                               >  1:2439872/1‑150 (MQ=255)
                  gtgtTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCGAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTCAGCAGCggg                                                                                                                                              <  2:3688831/150‑1 (MQ=255)
                    gtTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTCAGCAGCGGGtt                                                                                                                                            <  1:3726889/150‑1 (MQ=255)
                     tttAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTTTTTTGGTTCAGCCACGGGTTg                                                                                                                                           >  2:2412517/1‑150 (MQ=255)
                         gTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGGTTTGTTTGGCGTCCGCAGggggggtggcc                                                                                                                                       >  2:2786522/1‑143 (MQ=255)
                           ttGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCT‑cc                                                                                                                                    <  1:375949/150‑1 (MQ=255)
                            tGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGGTTTTTTTTTGTTTCA‑CAGCGGGGTGCGCc‑ccc                                                                                                                                   >  2:3066070/1‑150 (MQ=255)
                             gCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTTTTTTGGTTCAGCAGCCGGGTTCGGT‑CCCt                                                                                                                                  >  1:1564924/1‑150 (MQ=255)
                              cTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCT‑CCCtt                                                                                                                                 <  1:882481/150‑1 (MQ=255)
                              cTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCT‑CCCtt                                                                                                                                 <  1:2709284/150‑1 (MQ=255)
                              cTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCT‑CCCtt                                                                                                                                 <  1:2518100/150‑1 (MQ=255)
                              cTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCT‑CCCtt                                                                                                                                 <  1:1362808/150‑1 (MQ=255)
                                 ggTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCT‑CCCTTA‑At                                                                                                                             <  2:1339659/150‑1 (MQ=255)
                                 ggTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCT‑CCCTTA‑At                                                                                                                             <  1:1191341/150‑1 (MQ=255)
                                   tAAAGCGCCGTCGGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCT‑CCCTTA‑Atgt                                                                                                                           <  2:4121662/150‑1 (MQ=255)
                                   tAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTTTTTGGCGTCACCAGCGGGTTTCGCT‑ccccttattt                                                                                                                            >  2:1510507/1‑143 (MQ=255)
                                   tAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTTGCTTCAGCAGCGGGGTGCGCT‑CCCTTT‑Atgt                                                                                                                           >  1:2713182/1‑150 (MQ=255)
                                        cgcCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCT‑CCCTTA‑ATGTGCCTc                                                                                                                      <  1:2780459/150‑1 (MQ=255)
                                         gcCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTTTTTTGGTTCCGCAGCGGGGTGCGCC‑CCCCTT‑ATTTGGCTcc                                                                                                                     >  2:299230/1‑149 (MQ=255)
                                         gcCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTTTTTGGCTTTCGCAGCGGGGTGCGCC‑CCCTTT‑ATGTTCCCCg                                                                                                                     >  2:3386402/1‑150 (MQ=255)
                                            gTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCT‑CCCTTA‑ATGTGCCTCGCCa                                                                                                                  <  1:3466377/150‑1 (MQ=255)
                                            gTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCT‑CCATTA‑ATGTGCCTCGCCa                                                                                                                  <  1:1290517/150‑1 (MQ=255)
                                              cAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCT‑CCCTTA‑ATGTGCCTCGCCata                                                                                                                <  1:3007073/150‑1 (MQ=255)
                                              cAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGCTTTTTTTTTCTTCCGCAGCGGGGTGCGCT‑CCCCTA‑ATATGCCTccccccc                                                                                                                >  2:2757409/1‑147 (MQ=255)
                                               aGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCT‑CCCTTA‑ATGTGCCTCGCCata‑t                                                                                                              <  1:3503929/150‑1 (MQ=255)
                                                 cggcgATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTTTTTTGGTTCAGCCGCGGGGTGCGCC‑CCCTTT‑ATTTGCCTCCCCCTA‑T‑aa                                                                                                           >  2:3481330/1‑150 (MQ=255)
                                                   gcgATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCT‑CCCTTA‑ATGTGCCTCGCCATA‑T‑AAAt                                                                                                         <  2:1216348/150‑1 (MQ=255)
                                                    cgATGCAAAAACGCTTTGCCGAGGCCGGTGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCT‑CCCTTA‑ATGTGCCTCGCCATA‑T‑AAAtt                                                                                                        <  1:3399023/150‑1 (MQ=255)
                                                     gATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCT‑CCCTTA‑ATGTGCCTCGCCATA‑T‑AAATTg                                                                                                       <  2:2439872/150‑1 (MQ=255)
                                                       tGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTCCGCAGCGGGGTGCGCT‑CCCCTT‑ATGTGCCTCGCCCTA‑T‑AAAtttga                                                                                                     >  1:3305891/1‑147 (MQ=255)
                                                       tGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCT‑CCCTTA‑ATGTGCCTCGCCATA‑T‑AAATTGaa                                                                                                     <  2:113039/150‑1 (MQ=255)
                                                         cAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCT‑CCCTTA‑ATGTGCCTCGCCATA‑T‑AAATTGAATg                                                                                                   <  2:131358/150‑1 (MQ=255)
                                                           aaaaCGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCT‑CCCTTA‑ATGTGCCTCGCCATA‑T‑AAATTGAATGGt                                                                                                 <  1:2192551/150‑1 (MQ=255)
                                                            aaaCGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCT‑CCCTTA‑ATGTGCCTCGCCATA‑T‑AAATTGAATGGTg                                                                                                <  2:2369584/150‑1 (MQ=255)
                                                            aaaCGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCT‑CCCTTA‑ATGTGCCTCGCCATA‑T‑AAATTGAATGGTg                                                                                                <  1:785480/150‑1 (MQ=255)
                                                               cGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTTTTTTGCTTCAGCAGCGGGGTGGGCT‑CCCCTT‑ATGTGGCTCGCCCAA‑T‑AAATTTAATgggggag                                                                                             >  2:1629073/1‑145 (MQ=255)
                                                               cGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCT‑CCCTTA‑ATGTGCCTCGCCATA‑T‑AAATTGAATGGTGCAg                                                                                             <  1:556507/150‑1 (MQ=255)
                                                                      ccGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCT‑CCCTTA‑ATGTGCCTCGCCATA‑T‑AAATTGAATGGTGCAGGGAgcgc                                                                                      <  2:1904840/150‑1 (MQ=255)
                                                                          ggCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCT‑CCCTTA‑ATGTGCCTCGCCATA‑T‑AAATTGAATGGTGCAGGGAGCGCG‑CAg                                                                                 <  2:25101/150‑1 (MQ=255)
                                                                             cGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCT‑CCCTTA‑ATGTGCCTCGCCATA‑T‑AAATTGAATGGTGCAGGGAGCGCG‑CAgggg                                                                              <  1:948596/150‑1 (MQ=255)
                                                                               aTGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGGTTTTTTTTGCTTTAGCAGCGGGGTGCGGT‑CCCCTT‑ATGTGCCCCCCCCTA‑T‑AAATTTAATGGTGGAAGGGGGGCG‑CAgggggg                                                                            >  2:970140/1‑149 (MQ=255)
                                                                                tGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTTTTTTTGTTTTGCAGCGGGGTTTGCTCCCCTTA‑TTTTGCCCCCCCCCT‑T‑AAATTAAAT‑GTGTGGGGGGGGCG‑CCggggggg                                                                           >  2:4277709/1‑148 (MQ=255)
                                                                                 gcgcACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCT‑CCCTTA‑ATGTGCCTCGCCATA‑T‑AAATTGAATGGTGCAGGGAGCGCG‑CAGGGggcgg                                                                          <  1:1648635/150‑1 (MQ=255)
                                                                                  cgcACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTTGGTTCAGCAGCGGGGTGCGCC‑CCCCTT‑ATGTGCCTCCCCCTA‑T‑AAATTTAATGGTGCAGGGGGGGCG‑CCGGGgggggc                                                                         >  2:81392/1‑150 (MQ=255)
                                                                                      cTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTTTTTTGCTTTAGCAGCGGGGTGGGCT‑CCCCTT‑ATTTGCCTCGCCCTA‑T‑AAATTTAATGGTGGAGGGGGGGCG‑CGGGGGGGGGGCaaa                                                                     >  2:462083/1‑149 (MQ=255)
                                                                                           aTTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTTTTTTGCTTCAGCAGCGGGGTGCGCC‑CCCCTA‑ATTTGCCCCCCCCTA‑T‑AAAATTAATGGGGGAGGGGGGGCG‑CAGGGGGGGGCCCA‑Acgccg                                                               >  2:357925/1‑150 (MQ=255)
                                                                                             tCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCT‑CCCTTA‑ATGTGCCTCGCCATA‑T‑AAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAA‑Tcgccgcc                                                             <  2:264246/150‑1 (MQ=255)
                                                                                                 cTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCT‑CCCTTA‑ATGTGCCTCGCCATA‑T‑AAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAA‑TCGCCGCCGccc                                                         <  2:442488/150‑1 (MQ=255)
                                                                                                      ccgTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTTTTTTGCTTCAGCAGCGGGGTGCGCT‑CCCCTT‑ATTTGCCTCGCCCTA‑T‑AAATTTAATGGGGCGGGGGGGGCG‑CCGGGGGGGGCCCA‑ACGCccccccccccccc                                                    >  2:747435/1‑147 (MQ=255)
                                                                                                        gTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTTTTTTGCTTTAACAGCGGGGTGCGGTCCCCTTA‑TTTTGCC‑CGCCCCC‑T‑AAAATTAATGGGGCAGGGGGGGCG‑CCGGGGGGGGCCCA‑ACGCccccccccccccccg                                                  >  2:14525/1‑145 (MQ=255)
                                                                                                         ttaCGGTTGCAGGAAACCTAAGCGACTTAGCATGCGGC‑GGTTTTGTTTGGCTTCAGCAGTGGGTTGCGCT‑CCCTTA‑ATGTGCCTCGCCATA‑T‑AAATTGAATGGTGAAGGGAGCGGG‑CAGGGGGCGGCCAA‑TCGAAGCCGTCCCctg‑ct‑ga                                               <  2:3096866/148‑2 (MQ=255)
                                                                                                          cACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTTTTTGGGTTTAGCAGCGGGGTGGGCT‑CCCCTT‑ATGTGGCTCGCCCTA‑T‑AAAATTAATGGGGCAGGGGGGGCG‑CAGGGGGGGGGCAA‑TCGCCCCCCCCCCCCC‑CT‑G‑Tc                                             >  2:2215487/1‑150 (MQ=255)
                                                                                                            cGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTTTTTTGGTTTAGCAGCGGGGTGGGCT‑CCCCTT‑AAGTGGCTCGCCCCA‑TAAAATTGATTGG‑GGAGGGGGGGCG‑CCGGGGGGGGGCCA‑ACCCCGCCCCCCCCCT‑CC‑G‑Tccc                                           >  2:2811875/1‑150 (MQ=255)
                                                                                                              gTTGCAGGAAAGCTCAGGGCCTCAGGTTGGGGC‑GGTTTTGTTTGGCTTCCGCAGCGGGTTGCGGC‑CCCTTA‑ATGTGCGTCGCCATA‑T‑AAATTGATTGGTGCAGGGGGCGCG‑CGGGGGGCGGCCAA‑TCGCCGCCGCCCCCTG‑TT‑G‑TCCCgg                                         <  2:3630737/150‑1 (MQ=255)
                                                                                                                tGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTTTTTTGGTTCAGCAGCGGGGTGCGCT‑CCCTTA‑ATGTGGCTCGCCCTA‑T‑AAATTGAATTGTGGAGGGGGCGCG‑CAGGGGGCGGCCAA‑TCGCCGCCGCCCCCCG‑CT‑G‑TCCCCGcc                                       >  2:678909/1‑150 (MQ=255)
                                                                                                                tGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGGTTTTTTTTGGTTCCGCAGCGGGGTGCGCT‑CCCCTT‑ATATGCCCCCCCCCA‑T‑AAATTTAAATGTGGAAGGGGGGCG‑CAGGGGGGGGCCCA‑ACCCCCCCCCCCCCCC‑CT‑T‑TCCCCGcc                                       >  2:2429035/1‑150 (MQ=255)
                                                                                                                  cAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCT‑CCCTTA‑ATGTGCCTCGCCATA‑T‑AAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAA‑TCGCCGCCGCCCCCTG‑CT‑G‑TCCCGGCCtt                                     <  2:2857918/150‑1 (MQ=255)
                                                                                                                   aGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTTGGCTCCGACGCGGGTTGTGCT‑CCCTTC‑ATGTGCCTCCCCCCA‑T‑AAAATGAATGGTGCAGGGAGCGCC‑CACGGGGCGGGCAACTCCCCCCCCCCCCCTC‑CG‑G‑CCCCGGCCtc                                     >  2:1884927/1‑149 (MQ=255)
                                                                                                                     gAAAGCTAAGGGACTTAGCCTGCGGC‑GGGTTTTTTTTGCCTCAAGAGCGGGGGGCGGT‑CCCCTA‑ATTGGGCCCGCCCTA‑T‑AAAATTAATGGGGCAGGGGGCGCG‑CCGGGGGGGGCCCA‑ACCCCCCCGCCCCCCGCCG‑G‑TCCCGCCCttgg                                   >  2:1174456/1‑148 (MQ=255)
                                                                                                                      aaaGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGGTTTGCTTTCCCATCGGGGTGCGCT‑CCCCTACAATTGCC‑CGCCCAA‑T‑AAATTTTATTGTGCAGGGGGCGGG‑CCAGGGGCGGGCCA‑ACACCCCCGCCCCCCC‑CTCG‑CCCCGGCCtcggg                                  >  2:2105803/1‑146 (MQ=255)
                                                                                                                       aaGCTAAGGGACTTAGCCTGCGGC‑GGTTTTTTTTTGCTTCAGCAGCGGGGTGGGCT‑CCCCTA‑ATGTGCCTCGCCCTA‑T‑AAATTTAATGGGGGAGGGGGGGGG‑CCGGGGGGGGCCCA‑ACGCCGCCGCCCCCCG‑CC‑GTTCCCGGCC‑TCgggg                                >  2:3605026/1‑150 (MQ=255)
                                                                                                                       aaGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCT‑CCCTTA‑ATGTGCCTCGCCATA‑T‑AAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAA‑TCGCCGCCGCCCCCTG‑CT‑G‑TCCCGGCCTTCgggg                                <  1:1182199/150‑1 (MQ=255)
                                                                                                                        aGCTAAGGGACTTAGCCTGCGGC‑GGTTTTTTTTTGCTTCAGCAGCGGGGTTCGCC‑CCCCTT‑ATTTGCCTCCCCCTT‑T‑AAATTTAATGGGGGCGGGGGGGCG‑CCGGGGGGGGGCCA‑ACGCCCCCCGCCCCCG‑CC‑T‑TCCCCGCCCTCggggg                               >  2:3969149/1‑149 (MQ=255)
                                                                                                                         gCTAAGGGACTTAGCCTGCGGC‑GGTTTTTTTTTGCTTTAGCAGCGGGGTGCGCT‑CCCCTA‑ATTTGCCCCGCCCCA‑T‑AAAATTAATGGTGCAGGGGGCGCG‑CAGGGGGGGGCCCA‑TCCCCCCCCCCCCCCG‑CT‑T‑TCCCCGCCTTTggggga                              >  2:3365952/1‑148 (MQ=255)
                                                                                                                         gCTAAGGGACTTAGCCTGCGGC‑GGGTTTTTTTGGGTTCAGCAGCGGGGGGCGCT‑CCCCTA‑ATGTGGCTCGCCCTA‑T‑AAATTTAATGGGGCGGGGGGCGCG‑CCGGGGGGGGGCAA‑TCGCCGCCCCCCCCCG‑CT‑G‑TCCCGGCCCTCgggggg                              >  2:562756/1‑148 (MQ=255)
                                                                                                                            aaGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCT‑CCCTTA‑ATGTGCCTCGCCATA‑T‑AAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAA‑TCGCCGCCGCCCCCTG‑CT‑G‑TCCCGGCCTTCGGGGAACGc                           <  1:703272/150‑1 (MQ=255)
                                                                                                                             aGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCT‑CCCTTA‑ATGTGCCTCGCCATA‑T‑AAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAA‑TCGCCGCCGCCCCCTG‑CT‑G‑TCCCGGCCTTCGGGGAACGCt                          <  2:709943/150‑1 (MQ=255)
                                                                                                                              gggACTTAGCCTGCGGC‑GGTTTTTTTTTGCTTCAGCAGCGGGGTGCGCC‑CCCCTT‑ATGTGCCCCGCCCTA‑T‑AAATTTAATGGTGCAGGGAGCGCG‑CCGGGGGCGGGCCA‑TCCCCGCCCCCCCCCG‑CC‑G‑TTCCCGCCCTCCGGGGACGcct                         >  2:1651128/1‑148 (MQ=255)
                                                                                                                               ggACTTAGCCTGCGGC‑GGTTTTTTTTTGGTTTAGCCGCGGGGTGTGCTCCCCTTA‑AT‑TGCCCCCCCCTATT‑AATTTTATTGGTGC‑GGGGGCGCGCCCGGGGGGGGCCAA‑CCCCCCCCCCCCCCCC‑CG‑G‑CCCCGGCCTCGGGGGaagcctc                         >  2:1959861/1‑145 (MQ=255)
                                                                                                                                 aCTTAGCCTGCGGC‑GGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCT‑CCCTTA‑ATGTGCCTCGCCATA‑T‑AAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAA‑TCGCCGCCGCCCCCTG‑CT‑G‑TCCCGGCCTTCGGGGAACGCTTCAg                      <  1:2898393/150‑1 (MQ=255)
                                                                                                                                         tGCGGC‑GGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCT‑CCCTTA‑ATGTGCCTCGCCATA‑T‑AAATTGAATGGTGCAGGGAGCGCG‑CAGAGGGCGGCCAA‑TCGCCGCCGCCCCCTG‑CT‑G‑TCCCGGCCTTCGGGGAACGCTTCAGCGATTTTg              <  1:1770536/150‑1 (MQ=255)
                                                                                                                                           cggc‑ggTTTTGTTTGGCTTCAGCAGCGGGTTGCGCT‑CCCTTA‑ATGTGCCTCGCCATA‑T‑AAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAA‑TCGCCGCCGCCCCCTG‑CT‑G‑TCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGAc            <  2:2588619/150‑1 (MQ=255)
                                                                                                                                            ggc‑ggTTTTGTTTGGCTTCAGCAGCGGGTTGCGCT‑CCCTTA‑ATGTGCCTCGCCATA‑T‑AAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAA‑TCGCCGCCGCCCCCTG‑CT‑G‑TCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACg           >  1:1147553/1‑150 (MQ=255)
                                                                                                                                                ggTTTTGTTTGGCTTCAGCAGCGGGTTGCGCT‑CCCTTA‑ATGTGCCTCGCCATA‑T‑AAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAA‑TCGCCGCCGCCCCCTG‑CT‑G‑TCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGcca        >  1:3325643/1‑150 (MQ=255)
                                                                                                                                                ggTTTTGTTTGGCTTCAGCAGCGGGTTGCGCT‑CCCTTA‑ATGTGCCTCGCCATA‑T‑AAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAA‑TCGCCGCCGCCCCCTG‑CT‑G‑TCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGcca        <  2:1800904/150‑1 (MQ=255)
                                                                                                                                                ggTTTTGTTTGGCTTCAGCAGCGGGTTGCGCT‑CCCTTA‑ATGTGCCTCGCCATA‑T‑AAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAA‑TCGCCGCCGCCCCCTG‑CT‑G‑TCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGcca        >  2:2624866/1‑150 (MQ=255)
                                                                                                                                                 gTTTTGTTTGGCTTCAGCAGCGGGTTGCGCT‑CCCTTA‑ATGTGCCTCGCCATA‑T‑AAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAA‑TCGCCGCCGCCCCCTG‑CT‑G‑TCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGccac       >  1:2364884/1‑150 (MQ=255)
                                                                                                                                                 gTTTTGTTTGGCTTCAGCAGCGGGTTGCGCT‑CCCTTA‑ATGTGCCTCGCCATA‑T‑AAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAA‑TCGCCGCCGCCCCCTG‑CT‑G‑TCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGccac       <  2:2620208/150‑1 (MQ=255)
                                                                                                                                                    ttgtttgGCTTCAGCAGCGGGTTGCGCT‑CCCTTA‑ATGTGCCTCGCCATA‑T‑AAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAA‑TCGCCGCCGCCCCCTG‑CT‑G‑TCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGCCACCaa    >  2:4021350/1‑150 (MQ=255)
                                                                                                                                                     tgtttgGCTTCAGCAGCGGGTTGCGCT‑CCCTTA‑ATGTGCCTCGCCATA‑T‑AAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAA‑TCGCCGCCGCCCCCTG‑CT‑G‑TCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGCCACCAac   >  1:3678574/1‑150 (MQ=255)
                                                                                                                                                     tgtttgGCTTCAGCAGCGGGTTGCGCT‑CCCTTA‑ATGTGCCTCGCCATA‑T‑AAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAA‑TCGCCGCCGCCCCCTG‑CT‑G‑TCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGCCACCAac   <  2:72754/150‑1 (MQ=255)
                                                                                                                                                      gtttgGCTTCAGCAGCGGGTTGCGCT‑CCCTTA‑ATGTGCCTCGCCATA‑T‑AAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAA‑TCGCCGCCGCCCCCTG‑CT‑G‑TCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGCCACCAaca  >  1:4224802/1‑150 (MQ=255)
                                                                                                                                                      gtttgGCTTCAGCAGCGGGTTGCGCT‑CCCTTA‑ATGTGCCTCGCCATA‑T‑AAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAA‑TCGCCGCCGCCCCCTG‑CT‑G‑TCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGCCACCAaca  <  1:1155307/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                              
GGCAGTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCT‑CCCTTA‑ATGTGCCTCGCCATA‑T‑AAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAA‑TCGCCGCCGCCCCCTG‑CT‑G‑TCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGCCACCAACA  >  AP012306/934905‑935203

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑