Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123061,128,4120TG22.3% 675.9 / 32.2 265V29G (GTG→GGG) yciOconserved protein
Reads supporting (aligned to +/- strand):  ref base T (48/158);  new base G (59/0);  total (107/158)
Fisher's exact test for biased strand distribution p-value = 1.15e-29
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 327 total aligned reads displayed.

CCGCAGGCGTTGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCG‑CTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAACTCTCGACCTATT  >  AP012306/1128263‑1128561
                                                                                                                                                     |                                                                                                                                                       
 cGCAGGCGTTGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTg                                                                                                                                                        <  2:1450174/150‑1 (MQ=255)
   cAGGCGTTGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGAt                                                                                                                                                      >  1:643477/1‑150 (MQ=255)
   cAGGCGTTGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGAt                                                                                                                                                      <  2:1148120/150‑1 (MQ=255)
   cAGGCGTTGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGAt                                                                                                                                                      >  1:1503265/1‑150 (MQ=255)
     ggCGTTGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTg                                                                                                                                                    >  2:2558605/1‑150 (MQ=255)
        gTTGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGGATGttt                                                                                                                                                 >  1:2053828/1‑150 (MQ=255)
        gTTGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGgtt                                                                                                                                                 >  2:58747/1‑150 (MQ=255)
         ttGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTa                                                                                                                                                <  1:1443173/150‑1 (MQ=255)
         ttGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTa                                                                                                                                                >  1:1709085/1‑150 (MQ=255)
            aaGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGGTTGTTTAttc                                                                                                                                             >  2:2774700/1‑148 (MQ=255)
            aaGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATcc                                                                                                                                             >  1:3239587/1‑150 (MQ=255)
            aaGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATcc                                                                                                                                             >  2:3718957/1‑150 (MQ=255)
                cGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGGTTGTTTTTCCCACt                                                                                                                                         >  2:2006351/1‑150 (MQ=255)
                 gTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTg                                                                                                                                        <  1:3267791/150‑1 (MQ=255)
                 gTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTTTCCAACTg                                                                                                                                        >  2:1921611/1‑150 (MQ=255)
                   cTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGAt                                                                                                                                      >  2:1208415/1‑150 (MQ=255)
                   cTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGAt                                                                                                                                      <  1:437447/150‑1 (MQ=255)
                    tGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAACTGAtt                                                                                                                                     >  1:1110955/1‑150 (MQ=255)
                        aGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAACTGATTCCgg                                                                                                                                 >  2:522528/1‑150 (MQ=255)
                          cTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCt                                                                                                                               <  2:4043540/150‑1 (MQ=255)
                             tGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATg                                                                                                                            >  1:2486494/1‑150 (MQ=255)
                              gggAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATgc                                                                                                                           <  2:1887150/150‑1 (MQ=255)
                                  aCAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAACTGATTCCGGCTATGGG‑CTc                                                                                                                      >  2:3323754/1‑150 (MQ=255)
                                    aGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCG‑CTCgg                                                                                                                    >  1:2971026/1‑150 (MQ=255)
                                     gCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCG‑CTCGGc                                                                                                                   >  1:4211518/1‑150 (MQ=255)
                                      ccGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCG‑CTCGGCt                                                                                                                  <  2:2590134/150‑1 (MQ=255)
                                         cAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGGGATCGTGCGTAAAGGCGGGGGGGTTGGTTTTTCAACTTATTCCGGCTATGCG‑ATCGGCT‑Gtt                                                                                                              >  2:4161860/1‑149 (MQ=255)
                                            aaCACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCG‑CTCGGCT‑GTaaaa                                                                                                           <  2:216924/150‑1 (MQ=255)
                                            aaCACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCG‑CTCGGCT‑GTaaaa                                                                                                           <  2:390673/150‑1 (MQ=255)
                                             acacCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCG‑CTCGGCT‑GTAAAAt                                                                                                          <  2:1549815/150‑1 (MQ=255)
                                             acacCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCG‑CTCGGCT‑GTAAAAt                                                                                                          <  1:2138726/150‑1 (MQ=255)
                                                ccacAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCG‑CTCGGCT‑GTAAAATTGa                                                                                                       <  1:763114/150‑1 (MQ=255)
                                                   caGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTTTCCCACTTATTTCCGCCATTCGCCCCGGTG‑GTAAAATTGaga                                                                                                     >  1:3173862/1‑148 (MQ=255)
                                                    agagagGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCG‑CTCGGCT‑GTAAAATTGAAGAt                                                                                                   >  1:23141/1‑150 (MQ=255)
                                                    agagagGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAACTGATTCCGGCTATGCG‑CTCGGCT‑GTAAAATTGAAGAt                                                                                                   >  2:136214/1‑150 (MQ=255)
                                                     gagagGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCG‑CTCGGCT‑GTAAAATTGAAGATa                                                                                                  <  2:2421132/150‑1 (MQ=255)
                                                     gagagGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGGTTGTTTTTCCCACTGATTTCGGCTATGCG‑CTCGGCTGGTAAAATT‑AAGATa                                                                                                  >  2:899071/1‑150 (MQ=255)
                                                      agagGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCG‑CTCGGCT‑GTAAAATTGAAGATaa                                                                                                 <  1:905834/150‑1 (MQ=255)
                                                      agagGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAACTGATTCCGGCTATGCG‑CTCGGCT‑GTAAAATTGAAGATaa                                                                                                 >  1:4035098/1‑150 (MQ=255)
                                                       gagGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCG‑CTCGGCT‑GTAAAATTGAAGATaaa                                                                                                <  2:2187224/150‑1 (MQ=255)
                                                        agGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCG‑CTCGGCT‑GTAAAATTGAAGATaaaa                                                                                               <  1:2423386/150‑1 (MQ=255)
                                                        agGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCG‑CTCGGCT‑GTAAAATTGAAGATaaaa                                                                                               <  2:1624322/150‑1 (MQ=255)
                                                           gAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCG‑CTCGGCT‑GTAAAATTGAAGATAAAAAcg                                                                                            <  1:3031786/150‑1 (MQ=255)
                                                           gAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGGTTGTTTATTCCACTGATTTCCGCTATGCG‑CTCGGGT‑GTTAAAATTAAAATaaaaaag                                                                                            >  1:1005440/1‑148 (MQ=255)
                                                            aaTTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAACCGATTCCCGCCATTCG‑GACGGCC‑TGAAAAATGAAGAAAAAAAcag                                                                                           >  2:1797351/1‑148 (MQ=255)
                                                             aTTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAACTGATTCCGGCTATGCG‑CTCGGCT‑GTAAAATTGAAGATAAAAAcgcg                                                                                          >  1:3686982/1‑150 (MQ=255)
                                                                aTGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCCGCTATGCG‑CTCGGCT‑GTAAAATTGAAGATAAAAACGCGATg                                                                                       <  2:879392/150‑1 (MQ=255)
                                                                 tGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTTTCCAACTGATTCCGGCTATGCG‑CTCGGCT‑GTAAAATTGAAGATAAAAACGCGATgg                                                                                      >  2:69743/1‑150 (MQ=255)
                                                                   aGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCG‑CTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAg                                                                                    <  1:294373/150‑1 (MQ=255)
                                                                   aGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTTTCCAACTGATTACGGCTATGCG‑CTCGGCT‑GTAAAATTGAAGATAAAAACACGATGGAg                                                                                    >  2:1327080/1‑150 (MQ=255)
                                                                        gTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCG‑CTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGTAt                                                                               <  1:3971474/150‑1 (MQ=255)
                                                                        gTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCG‑CTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGTAt                                                                               <  1:1660054/150‑1 (MQ=255)
                                                                        gTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGGTTGTTTATTCAACTGATTTCGGGTATGCG‑CTCGGCT‑GGAAAATTGAAGATAAAAAAGCGGTGGGGGGTAt                                                                               >  2:4197889/1‑150 (MQ=255)
                                                                         tttttttATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGAACGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCG‑CTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGTAtt                                                                              <  2:1259145/150‑1 (MQ=255)
                                                                           tttttATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAACTGATTCCGGCTATGCG‑CTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTg                                                                            >  1:687586/1‑150 (MQ=255)
                                                                           tttttATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAACTGATTCCGGCTATGCG‑CTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGGATTTg                                                                            >  1:1587262/1‑150 (MQ=255)
                                                                             tttATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCG‑CTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTc                                                                          <  2:1858244/150‑1 (MQ=255)
                                                                             tttATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCG‑CTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTc                                                                          <  2:659126/150‑1 (MQ=255)
                                                                              ttATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCG‑CTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCg                                                                         <  2:3097686/150‑1 (MQ=255)
                                                                              ttATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGGTTATCCAACTGATTCCGGCTATGCG‑CTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGGGTATTTGtgg                                                                         >  2:1075443/1‑148 (MQ=255)
                                                                                 tatTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCG‑CTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTAt                                                                      <  1:1808336/150‑1 (MQ=255)
                                                                                  atTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCG‑CTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTAtt                                                                     <  2:3168655/150‑1 (MQ=255)
                                                                                    tCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCG‑CTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCg                                                                   <  2:1966183/150‑1 (MQ=255)
                                                                                    tCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCG‑CTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCg                                                                   <  1:2203594/150‑1 (MQ=255)
                                                                                    tCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGGTTGTTTATTCAACTGATTTCGGCTTTGCG‑CTCGGGT‑GTAAAATTTGAGATAAAAACGCGATGGGGCGTATttttcgttttcg                                                                   >  2:3486275/1‑150 (MQ=255)
                                                                                      aTCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAACTGATTCCGGCTATGCG‑CTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGcc                                                                 >  2:3588014/1‑150 (MQ=255)
                                                                                        ccTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCG‑CTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAg                                                               <  2:1710857/150‑1 (MQ=255)
                                                                                              aaCCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTTATTGTTTATTCAACTGATTTCCGCTATGCG‑CTCGGCT‑GTGAAATTGTAGATTAAAACACGATGGGGCGGATTTGTCGTATTTGCCTGCGGCCg                                                         >  2:2251819/1‑150 (MQ=255)
                                                                                                 ccACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCG‑CTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGAc                                                      <  2:4261724/150‑1 (MQ=255)
                                                                                                 ccACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACCGATTCCGGCCAAGGC‑CTCGGCT‑GTCAAAGTGAAGGTAAAAAAGCGCAGGGGCGCGTTTGTCGTGATTGCCAGCTGCCGGAc                                                      >  2:2482149/1‑150 (MQ=255)
                                                                                                  cacaGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAACTGATTCCGGCTATGCG‑CTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGGCGGACg                                                     >  1:485797/1‑150 (MQ=255)
                                                                                                     aGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCG‑CTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTc                                                  <  2:3456235/150‑1 (MQ=255)
                                                                                                      gCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCG‑CTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTca                                                 <  1:3782231/150‑1 (MQ=255)
                                                                                                       cAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCG‑CTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTcac                                                <  2:2711232/150‑1 (MQ=255)
                                                                                                        aaCGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCG‑CTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTcaca                                               >  1:362301/1‑150 (MQ=255)
                                                                                                               gATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAACTGATTCCGGCTATGCG‑CTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTTTCGTATTCGCCAGCTGCCGGACGGGCACAACTTTAc                                        >  1:1284577/1‑150 (MQ=255)
                                                                                                                       aGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCG‑CTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGAtgt                                <  2:3264728/150‑1 (MQ=255)
                                                                                                                       aGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCCGCTATGCG‑CTCGGCT‑GGAAAATTGAAGATAAAAACGCGATGGGGCGGATTTGTCCCATTTGCCAGCTGCCGGACGGTGCCAACTTTAACCTGAtgt                                >  2:1820130/1‑150 (MQ=255)
                                                                                                                       aGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAACTGATTCCGGCTATGCG‑CTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGGGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGGCACAACTTTACCCTGAtgt                                >  2:3926406/1‑150 (MQ=255)
                                                                                                                        ggCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCG‑CTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGAtgtg                               <  1:975176/150‑1 (MQ=255)
                                                                                                                         gCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCG‑CTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATgtgt                              <  1:2341004/150‑1 (MQ=255)
                                                                                                                         gCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCG‑CTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATgtgt                              <  2:3792350/150‑1 (MQ=255)
                                                                                                                             tGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAACTGATTCCGGCTATGCG‑CTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTcgcg                          >  2:2127634/1‑150 (MQ=255)
                                                                                                                                agaTCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCG‑CTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATc                       <  1:2721521/150‑1 (MQ=255)
                                                                                                                                  aTCGTGCGTAAAGGCGGGGTGATTGTTTATCCAGCTGATTCCGGCTATGCG‑CTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCtt                     <  2:3449132/150‑1 (MQ=255)
                                                                                                                                      tGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCG‑CTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTg                 >  1:3926130/1‑150 (MQ=255)
                                                                                                                                       gCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCG‑CTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGa                <  1:2908211/150‑1 (MQ=255)
                                                                                                                                       gCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCG‑CTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGa                <  1:3086278/150‑1 (MQ=255)
                                                                                                                                        cGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCG‑CTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGaa               <  1:1554997/150‑1 (MQ=255)
                                                                                                                                        cGTAAAGGCGGGGGGATTGTTTATCCAACTGATTCCGGCTATGCG‑CTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGGGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGaa               >  2:4215068/1‑150 (MQ=255)
                                                                                                                                         gTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCG‑CTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAAc              <  2:2378850/150‑1 (MQ=255)
                                                                                                                                           aaaGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCG‑CTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAActc            >  2:3734357/1‑150 (MQ=255)
                                                                                                                                             aGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCG‑CTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAACtctc          <  1:3504783/150‑1 (MQ=255)
                                                                                                                                              ngCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCG‑CTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAACTCTCg         <  1:1189069/149‑1 (MQ=255)
                                                                                                                                              ggCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCG‑CTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAACTCTCg         <  1:4072146/150‑1 (MQ=255)
                                                                                                                                               gCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCG‑CTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAACTCTCGa        <  1:2222189/150‑1 (MQ=255)
                                                                                                                                               gCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCG‑CTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAACTCTCGa        <  1:2785936/150‑1 (MQ=255)
                                                                                                                                                cGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCG‑CTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAACTCTCGAc       <  2:77862/150‑1 (MQ=255)
                                                                                                                                                 ggggTGATTGTTTATCCAACTGATTCCGGCTATGCG‑CTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAACTCTCGAcc      >  2:2622110/1‑150 (MQ=255)
                                                                                                                                                  gggTGATTGTTTATCCAACTGATTCCGGCTATGCG‑CTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAACTCTCGACCt     <  1:2607253/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                       
CCGCAGGCGTTGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCG‑CTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAACTCTCGACCTATT  >  AP012306/1128263‑1128561

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑