Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123063,020,6700TG33.1% 298.8 / 10.3 170K195N (AAA→AAC) glgCglucose‑1‑phosphate adenylyltransferase
Reads supporting (aligned to +/- strand):  ref base T (71/42);  new base G (56/0);  total (128/42)
Fisher's exact test for biased strand distribution p-value = 2.20e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 228 total aligned reads displayed.

ATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTT‑AGCAGGTTTTTC‑AACGAA‑TTCGATAA‑TTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGAGTAGTCTTGCTT  >  AP012306/3020521‑3020819
                                                                                                                                                      |                                                                                                                                                        
    aaTCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGT‑AGCAGGgtttt                                                                                                                                                      >  1:4215322/1‑150 (MQ=255)
      tCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTT‑AGCAGGTTTTTC‑a                                                                                                                                                   <  1:3177183/150‑1 (MQ=255)
       cTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGGTACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGT‑AGCAGGGTTTTC‑aa                                                                                                                                                  >  1:751176/1‑150 (MQ=255)
       cTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTT‑AGCAGGTTTTTC‑aa                                                                                                                                                  >  1:3766048/1‑150 (MQ=255)
        tttGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTT‑AGCAGGTTTTTC‑AAc                                                                                                                                                 <  1:405352/150‑1 (MQ=255)
           gCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGT‑AGCAGGGTTTTC‑AACGaa                                                                                                                                              >  1:3240019/1‑150 (MQ=255)
           gCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGT‑AGCAGGGTTTTC‑AACGaa                                                                                                                                              >  1:3915252/1‑150 (MQ=255)
           gCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCATCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGT‑AGCAGGGTTTTC‑AACGaa                                                                                                                                              >  1:2923439/1‑150 (MQ=255)
             cAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTT‑AGCAGGTTTTTC‑AACGAA‑tt                                                                                                                                           <  1:202327/150‑1 (MQ=255)
               aaGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTT‑AGCAGGTTTTTC‑AACGAA‑TTCg                                                                                                                                         <  1:902002/150‑1 (MQ=255)
                aGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTT‑AGCAGGTTTTTC‑AACGAA‑TTCGa                                                                                                                                        <  2:1926997/150‑1 (MQ=255)
                 gTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTT‑AGCAGGTTTTTC‑AACGAA‑TTCGAt                                                                                                                                       <  2:1335468/150‑1 (MQ=255)
                   cGTGGCTGGAGTTCTCATCGCGATCGACTTCTTCCAGCAGTTCAAACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGCT‑TGCAGGGGTTTC‑AATGAG‑TTTGACaa                                                                                                                                     >  2:4107380/1‑150 (MQ=255)
                      ggCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTT‑AGCAGGTTTTTC‑AACGAA‑TTCGATAA‑ttt                                                                                                                                 <  1:3845619/150‑1 (MQ=255)
                       gCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTT‑AGCAAGATTTTT‑AACGAA‑TTCGATAA‑tttt                                                                                                                                >  2:143044/1‑150 (MQ=255)
                        cTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTT‑AGCAGGTTTTTC‑AACGAA‑TTCGATAA‑TTTTa                                                                                                                               <  1:2325385/150‑1 (MQ=255)
                        cTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGT‑AGCAGGGTTTTC‑AACGAA‑TTCGATAA‑TTTTa                                                                                                                               >  1:503116/1‑150 (MQ=255)
                         tGGAGTTCTTATCGCGATGGGCTTCTTCCAGCAGTTCATAGAGATAGTCGGCGTCAAAGACGTAGATAGCCATACTCGCCAGAGATTTGCGCGGCTTGTTCGGCATTGACGGCGGGTT‑AGCAGGTTTTTC‑AACGAA‑TTCGATAA‑TTTTAt                                                                                                                              <  1:1189664/150‑1 (MQ=255)
                         tGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTT‑AGCAGGTTTTTC‑AACGAA‑TTCGATAA‑TTTTAt                                                                                                                              <  1:4114125/150‑1 (MQ=255)
                         tGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTT‑AGCAGGTTTTTC‑AACGAA‑TTCGATAA‑TTTTAt                                                                                                                              <  2:3073788/150‑1 (MQ=255)
                         tGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTT‑AGCAGGTTTTTC‑AACGAA‑TTCGATAA‑TTTTAt                                                                                                                              <  1:1155563/150‑1 (MQ=255)
                           gAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTT‑AGCAGGTTTTTC‑AACGAA‑TTCGATAA‑TTTTATCg                                                                                                                            <  2:1202375/150‑1 (MQ=255)
                            aGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTT‑AGCAAGGTTTTC‑AACGAA‑TTCGATTA‑ATTTATCGt                                                                                                                           >  1:2219805/1‑150 (MQ=255)
                             gTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTT‑AGCAGGTTTTTC‑AACGAA‑TTCGATAA‑TTTTATCGtt                                                                                                                          <  2:2863245/150‑1 (MQ=255)
                               tctcATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTT‑AGCAGGTTTTTC‑AACGAA‑TTCGATAA‑TTTTATCGTtct                                                                                                                        <  2:1782089/150‑1 (MQ=255)
                                  cATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTT‑AGCAGGTTTTTC‑AACGAA‑TTCGATAA‑TTTTATCGTTCtcat                                                                                                                     <  1:2058231/150‑1 (MQ=255)
                                  cATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTT‑AGCAGGTTTTTC‑AACGAA‑TTCGATAA‑TTTTATCGTTCtcat                                                                                                                     >  1:353323/1‑150 (MQ=255)
                                    tCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTT‑AGCAGGTTTTTC‑AACGAA‑TTCGATAA‑TTTTATCGTTCtcatca                                                                                                                   >  1:1085695/1‑150 (MQ=255)
                                    tCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTT‑AGCAGGGTTTTC‑AACGAA‑TTCGATAA‑TTTTATCGTTCtcatca                                                                                                                   >  1:2399422/1‑150 (MQ=255)
                                     cgcgATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTT‑AGCAGGTTTTTC‑AACGAA‑TTCGATAA‑TTTTATCGTTCTCATCaa                                                                                                                  <  1:2551163/150‑1 (MQ=255)
                                      gcgATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTT‑AGCAGGTTTTTC‑AACGAA‑TTCGATAA‑TTTTATCGTTCTCATCAAc                                                                                                                 <  2:2370404/150‑1 (MQ=255)
                                       cgATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTT‑AGCAGGTTTTTC‑AACGAA‑TTCGATAA‑TTTTATCGTTCTCATCAAcc                                                                                                                <  1:3555985/150‑1 (MQ=255)
                                        gATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTT‑AGCAGGTTTTTT‑AACGAA‑TTCGATAA‑TTTTTTCGTTCTCATCAACCg                                                                                                               >  2:3386577/1‑150 (MQ=255)
                                        gATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTT‑AGCAGGTTTTTC‑AACGAA‑TTCGATAA‑TTTTATCGTTCTCATCAACCg                                                                                                               <  1:2796581/150‑1 (MQ=255)
                                        gATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTT‑AGCAGGGTTTTT‑AACGAA‑TTCGTTAA‑TTTTATCCTTTTCCTCAACCg                                                                                                               >  2:1470510/1‑150 (MQ=255)
                                        gATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGT‑AGCAGGGTTTTC‑AACGAA‑TTCGATAA‑TTTTATCGTTCTCATCAACCg                                                                                                               >  2:1816799/1‑150 (MQ=255)
                                         aTCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTT‑AGCAGGTTTTTC‑AACGAA‑TTCGATAA‑TTTTATCGTTCTCATCAACCGc                                                                                                              >  1:3717006/1‑150 (MQ=255)
                                         aTCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTT‑AGCAGGTTTTTC‑AACGAA‑TTCGATAA‑TTTTATCGTTCTCATCAACCGc                                                                                                              <  2:2485712/150‑1 (MQ=255)
                                         aTCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTT‑AGCAGGGTTTTT‑AACGAA‑TTCGATAA‑TTTTATCGTTCTCATCAACCGc                                                                                                              >  2:3231831/1‑150 (MQ=255)
                                          tCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTT‑AGCAGGTTTTTC‑AACGAA‑TTCGATAA‑TTTTATCGTTCTCATCAACCGcc                                                                                                             >  1:870426/1‑150 (MQ=255)
                                           cGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTT‑AGCAGGTTTTTC‑AACGAA‑TTCGATAA‑TTTTATCGTTCTCATCAACCGCCa                                                                                                            >  1:1824074/1‑150 (MQ=255)
                                            gTCTTCTTCCAGCAGTTCATACAGATAGGCGGCGAAAAAGACGTAGATCCCCATCCTCGCCAGAGTTTTGCTCGGATCGTTCGGCATTGACGGGGGGTT‑AGCAGTTTTTTC‑AACGAA‑TTCGATAT‑TTTTATCTTTCTCATCAACCGCCAt                                                                                                           <  2:3460500/150‑1 (MQ=255)
                                                  ttcCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTT‑AGCAGGGTTTTT‑AACGAA‑TTCGATAA‑TTTTATCGTTCTCATCAACCGCCATAACGcc                                                                                                     >  2:2715235/1‑150 (MQ=255)
                                                                 cAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTT‑AGCAGGGTTTTC‑AACGAA‑TTCGATAA‑TTTTATCGTTCTCATCAACCGCCCTAACGCCAAATGCGGAGGcttc                                                                                      >  2:4182347/1‑150 (MQ=255)
                                                                  aGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTT‑AGCAGGTTTTTC‑AACGAA‑TTCGATAA‑TTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGcttct                                                                                     >  1:2284593/1‑150 (MQ=255)
                                                                  aGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTT‑AGCAGGTTTTTC‑AACGAA‑TTCGATAA‑TTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGcttct                                                                                     >  2:358879/1‑150 (MQ=255)
                                                                  aGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTT‑AGCAGGGTTTTT‑AACGAA‑TTCTTTAA‑TTTTATCGTTCTCCTCAACCGCCCTAACGCCAAATGCGGAGGcttct                                                                                     >  1:3035326/1‑150 (MQ=255)
                                                                   gATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTT‑AGCAGGTTTTTC‑AACGAA‑TTCGATAA‑TTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGcttctt                                                                                    >  2:685810/1‑150 (MQ=255)
                                                                       gTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGT‑AGCAGGTTTTTC‑AACGAA‑TTCGATAA‑TTTTATCGTTCTCATCCACCGCCATAACGCCAAATGCGGAGGCTTCTTCAAt                                                                                >  1:424068/1‑150 (MQ=255)
                                                                        tCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGT‑AGCAGGGTTTTT‑AACGAA‑TTCGATAA‑TTTTATCGTTCTCATCAACCGCCCTAAAGCCAAATGCGGAGGCGTCTTCaaac                                                                               >  2:1256642/1‑148 (MQ=255)
                                                                            cGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTT‑AGCAGGGTTTTC‑AACGAA‑TTCGATAA‑TTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTTTTCAATCGGTa                                                                           >  2:1611462/1‑150 (MQ=255)
                                                                             gTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTT‑AGCAGGTTTTTC‑AACGAA‑TTCGATAA‑TTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTAc                                                                          >  1:2674242/1‑150 (MQ=255)
                                                                             gTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTT‑AGCAGGTTTTTC‑AACGAA‑TTCGATAA‑TTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCCGTAc                                                                          >  1:82852/1‑150 (MQ=255)
                                                                              tCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTT‑AGCAGGGTTTTC‑AACGAA‑TTCGATAA‑TTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACt                                                                         >  1:700713/1‑150 (MQ=255)
                                                                               aaaaGACGTAGATCCCCATCCCCGCCAGATTTTTGCCCGGTTCGTTCGGCTTGGCGGGGGGGTT‑AGCAGTTTTTTC‑AACAAA‑TTCGATAT‑TTTTATCTTTCTCACCACCCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTg                                                                        <  2:3370119/149‑1 (MQ=255)
                                                                                aaaGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTT‑AGCAGGTTTTTC‑AACGAA‑TTCGATAA‑TTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTgg                                                                       >  1:2735686/1‑150 (MQ=255)
                                                                                aaaGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTT‑AGCAGGGTTTTC‑AACGAA‑TTCGATAA‑TTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTgg                                                                       >  2:896884/1‑150 (MQ=255)
                                                                                   gACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGT‑AGCAGGGTTTTC‑AACGAA‑TTCGATAA‑TTTTTTCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCAt                                                                    >  2:834892/1‑150 (MQ=255)
                                                                                    aCGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTT‑AGCAGGGTTTTC‑AACGAA‑TTCGATAA‑TTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATa                                                                   >  1:2473233/1‑150 (MQ=255)
                                                                                       taaatccccTTACTCGCCAGGTTTTTGCTGGGATCTTTCGGCTTGGAGGGGGGGTT‑AGC‑GGTTTTTT‑AACGAATTTCGATAT‑TTTTGTCGTTCTCAACAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACaa                                                                <  2:2583076/144‑1 (MQ=255)
                                                                                       tAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTT‑AGCAGGTTTTTC‑AACGAA‑TTCGATAA‑TTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACaa                                                                >  1:2137982/1‑150 (MQ=255)
                                                                                        aGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGT‑AGCAGGGTTTTC‑AACGAA‑TTCGATAA‑TTTTTTCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAg                                                               >  2:2217114/1‑150 (MQ=255)
                                                                                              caatccTCGCGAGAGTTTTGCGGGGATCGTTCGGCATGGGCGGGGGGTT‑AGCAGTTTTTTA‑AAAGAA‑TTCGATAT‑TTTTATCTTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACgg                                                         <  2:2322000/145‑1 (MQ=255)
                                                                                                aTACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTT‑AGCAGGGTTTTC‑AACGAA‑TTCGATAA‑TTTTATCGTTCTCATCAACCGCCCTAACGCCAAATGCGGAGGCTTCTTCAATCGGGACTGGCATACAAGCAACGGTg                                                       >  2:3954183/1‑150 (MQ=255)
                                                                                                   cTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTT‑AGCAGGGTTTTT‑AACGAA‑TTCGATAA‑TTTTATCGTTCTCATCAACCGCCATAACGCCAAAAGCGGAGGCTTCTTTAATCGGTACTGGCATACAAACAACGGTGCaa                                                    >  2:2777296/1‑150 (MQ=255)
                                                                                                     cGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTT‑AGCAGGTTTTTC‑AACGAA‑TTCGATAA‑TTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACg                                                  >  1:2881982/1‑150 (MQ=255)
                                                                                                     cGCAAGAGTTTTGCTCGGTTTGTTGGGTGTTGGCGGGGGGTT‑AGCAGTTTTTAA‑AAAAAA‑TTCTATAT‑TTTTATCTTTCTCACCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACg                                                  <  2:3509523/150‑1 (MQ=255)
                                                                                                         agagATTGCCGCGGATGGTCCGGAATGGA‑GGGGGGTT‑AAGAAGTTTTTT‑AAAGAATTTATATAT‑TTTTTTCGTTCTCATCAGCCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTgcg                                              <  2:3766898/150‑1 (MQ=255)
                                                                                                              tttGCTCGGTTGGTGCGTCATTGAGGGGGGGTT‑AGTTGTTTTTAC‑AAAGAA‑TTCGATAT‑TTTTATCTTTTTCATAAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCttt                                         <  2:2961785/150‑1 (MQ=255)
                                                                                                                  cTCGGTTCGTTCGGCTTGGGCGGGGGTTT‑AGC‑GGTTTTTCAAACAAT‑TTCAATAT‑TTTTCTCGTCCTCACCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCg                                     <  2:2916538/150‑1 (MQ=255)
                                                                                                                   tCGGATCGTTCGGCATTGACGGCGGGTT‑AGCAGGTTTTTC‑AACGAA‑TTCGATAA‑TTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCCTTTTCGa                                    >  1:1894127/1‑150 (MQ=255)
                                                                                                                   tCGGATCGTTCGGCATTGACGGCGGGTT‑AGCAGGGTTTTC‑AACGAA‑TTCGATAA‑TTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGCGCAACGTGCGCCTTTTTTGa                                    >  2:3691702/1‑150 (MQ=255)
                                                                                                                     ggATCGTTCGGCATTGACGGCGGGTT‑AGCAGGGTTTTC‑AACGAA‑TTCGATAA‑TTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACg                                  >  1:168597/1‑150 (MQ=255)
                                                                                                                     ggATCGTTCGGCATTGACGGCGGGTT‑AGCAGGGTTTTC‑AACGAA‑TTCGATAA‑TTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACg                                  >  2:332193/1‑150 (MQ=255)
                                                                                                                      gATCGTTCGGCATTGACGGCGGGTT‑AGCAGGTTTTTC‑AACGAA‑TTCGATAA‑TTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGt                                 >  1:2524029/1‑150 (MQ=255)
                                                                                                                      gATCGTTCGGCATTGACGGCGGGTT‑AGCAGGTTTTTC‑AACGAA‑TTCGATAA‑TTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGt                                 >  1:18663/1‑150 (MQ=255)
                                                                                                                      gATCGTTCGGCATTGACGGCGGGTT‑AGCAGGGTTTTC‑AACGAA‑TTCGATAA‑TTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGt                                 >  2:523469/1‑150 (MQ=255)
                                                                                                                      gATCGTTCGGCATTGACGGCGGGGT‑AGCAGGGTTTTC‑AACGAA‑TTCGATAA‑TTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGt                                 >  2:3858365/1‑150 (MQ=255)
                                                                                                                       aTCGTTCGGCATTGACGGCGGGTT‑AGCAGGTTTTTC‑AACGAA‑TTCGATAA‑TTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTg                                >  2:4061467/1‑150 (MQ=255)
                                                                                                                       aTCGTTCGGCATTGACGGCGGGTT‑AGCAGGTTTTTC‑AACGAA‑TTCGATAA‑TTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTg                                >  1:2969173/1‑150 (MQ=255)
                                                                                                                           ttCGGCATTGACGGCGGGTT‑AGCAGGTTTTTC‑AACGAA‑TTCGATAA‑TTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCg                            >  1:1337637/1‑150 (MQ=255)
                                                                                                                            tCGGCATTGACGGCGGGTT‑AGCAGGTTTTTC‑AACGAA‑TTCGATAA‑TTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGa                           >  1:331756/1‑150 (MQ=255)
                                                                                                                              ggCATTGACGGCGGGTT‑AGCAGGGTTTTC‑AACGAA‑TTCGATAA‑TTTTATAGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCTACGTGATCGATa                         >  2:3892707/1‑150 (MQ=255)
                                                                                                                                   ggggggggggTT‑AGGTGTTTTTTA‑AAAAAA‑TTAGTTTT‑TTTTTTCTTTCTCAAAACCCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCAt                    <  2:4021966/143‑1 (MQ=255)
                                                                                                                                   ggcgggggggTA‑AGCTGTTTTTTC‑AAAAAA‑TTTTTTTT‑TTTTTTCGTTCTCAACAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCAt                    <  2:3722954/143‑1 (MQ=255)
                                                                                                                                      cggcggGTT‑AGCAGGGTTTTC‑AACGAA‑TTCGAAAA‑TTTTTTCGGTCTCATCAACCGCCATAAAGCCCAAAGCGGAGGCTTCTTCAATCGGTACTTGGATACAAGCAACGGTGCAACCTGCGCCTTTTTGGCCGGGATCGATAACCATAcg                 >  2:1353545/1‑150 (MQ=255)
                                                                                                                                          gggTTAAGCAGGTTTTAC‑AAGGAT‑TTCGATAT‑TTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGa              <  1:15745/150‑1 (MQ=255)
                                                                                                                                          gggTT‑AGATGTTTTTAC‑AAC‑AA‑TTTTATAATTTTTCTCTTTCTCACCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGag             <  2:3636033/150‑1 (MQ=255)
                                                                                                                                                aGCAGGTTTTTC‑AACGAA‑TTCGATAA‑TTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGAGTAGTc        >  1:1496436/1‑150 (MQ=255)
                                                                                                                                                 tgtggTTTTAC‑AACAAT‑TTGTTTAT‑TTTTCTCTTTCTCAACAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGAGTAGTct       <  2:2631530/147‑1 (MQ=255)
                                                                                                                                                 gtagttttttA‑AAAGAT‑TTCGATAT‑TTTTATCTTTCTAACCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGAGTAGTct       <  2:2843575/145‑1 (MQ=255)
                                                                                                                                                 gCAGGTTTTTC‑AACGAA‑TTCGATAA‑TTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGAGTAGTct       >  2:1976764/1‑150 (MQ=255)
                                                                                                                                                  tagttttttA‑AACAAA‑TTCGATAT‑TTTTTTCTTTCTCACCACCCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGAGTAGTctt      <  2:153835/146‑1 (MQ=255)
                                                                                                                                                  cAGGTTTTTC‑AACGAA‑TTCGATAA‑TTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGAGTAGTctt      >  1:364119/1‑150 (MQ=255)
                                                                                                                                                   aGGTTTTTC‑AACGAA‑TTCGATAA‑TTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGAGTAGTcttg     >  1:2380832/1‑150 (MQ=255)
                                                                                                                                                   aGGTTTTTC‑AACGAA‑TTCGATAA‑TTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGAGTAGTcttg     >  1:3359002/1‑150 (MQ=255)
                                                                                                                                                    ttttttaC‑AACAAT‑TTAGATTT‑TTTTTTCTTTCTCACCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGAGTAGTcttgc    <  2:1687779/148‑1 (MQ=255)
                                                                                                                                                     ttttttA‑AACGAA‑TTAGTTTT‑TTTTTTCTTTCTCATCACCCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGAGTAGTcttgct   <  2:2142603/149‑1 (MQ=255)
                                                                                                                                                      tttttC‑AACGAA‑TTCGATAA‑TTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGAGTAGTcttgctt  >  1:1047303/1‑150 (MQ=255)
                                                                                                                                                      ttttaC‑AACAAA‑TTCAATAT‑TTTTATCTTTCTCACCACCCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGAGTAGTcttgctt  <  2:2349812/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                        
ATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTT‑AGCAGGTTTTTC‑AACGAA‑TTCGATAA‑TTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGAGTAGTCTTGCTT  >  AP012306/3020521‑3020819

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑