Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123063,182,8610AC26.9% 305.1 / 21.5 130L198F (TTA→TTC) yiaNpredicted transporter
Reads supporting (aligned to +/- strand):  ref base A (80/15);  new base C (0/35);  total (80/50)
Fisher's exact test for biased strand distribution p-value = 3.72e-20
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 204 total aligned reads displayed.

ATTATTCCCCCTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACC‑TGGTGGT‑GG‑CAGGCCA‑GTCGCC‑TTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCT‑ACCGAAGCCGGGGCCGTTGCTGCTTTCTATGC  >  AP012306/3182712‑3183010
                                                                                                                                                          |                                                                                                                                                      
attattCCCCCTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACC‑TGGTGGT‑GG‑CAGGCCA‑GTCGCC‑TTAATTTa                                                                                                                                                        <  1:553987/150‑1 (MQ=255)
 ttattCCCCCTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACC‑TGGTGGT‑GG‑CAGGCCA‑GTCGCC‑TTAATTTAc                                                                                                                                                       <  1:1541072/150‑1 (MQ=255)
 ttattCCCCCTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACC‑TGGTGGT‑GG‑CAGGCCA‑GTCGCC‑TTAATTTAc                                                                                                                                                       <  1:672493/150‑1 (MQ=255)
 ttattCCCCCTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACC‑TGGTGGT‑GG‑CAGGCCA‑GTCGCC‑TTAATTTAc                                                                                                                                                       <  1:1896802/150‑1 (MQ=255)
    ttCCCCCTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACC‑TGGTGGT‑GG‑CAGGCCA‑GTCGCC‑TTAATTTAcccc                                                                                                                                                    <  2:4108757/150‑1 (MQ=255)
    ttCCCCCTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACC‑TGGTGGT‑GG‑CAGGCCA‑GTCGCC‑TTAATTTAcccc                                                                                                                                                    <  1:3284517/150‑1 (MQ=255)
     tCCCCCTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACC‑TGGTGGT‑GG‑CAGGCCA‑GTCGCC‑TTAATTTACCCCg                                                                                                                                                   <  1:2658959/150‑1 (MQ=255)
      cccccTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCCCGCCTGATGGTGGGGGCCACGCTGATGGTTTCC‑TGGTGGG‑GG‑GCGGGCC‑GGCGCC‑CTTATTTTcccccc                                                                                                                                                  >  2:2233462/1‑148 (MQ=255)
        cccTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCCTTTCCCCCCGCATGATGATGGGGGCAACGCTGATGCTTACC‑TGGTGGT‑GG‑GGGGGCA‑GTCGCCTTTATTTTACcccccc                                                                                                                                                 >  2:3616140/1‑150 (MQ=255)
               aTTCCTTTTATTATCTCCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACC‑TGGTGGT‑GG‑CAGGCCA‑GTCGCC‑TTAATTTCCCCCGCCAGCaaaaa                                                                                                                                         <  2:1474190/150‑1 (MQ=255)
                 tCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTTCCCCCGGCCTTGTGATGGGGGCCAAGCTGGTGCTTTCCTTGGGGGG‑GG‑GGGGCCG‑GCGCCC‑TTATTTTTCCCCCCCGCCaaaaaa                                                                                                                                        >  2:4270444/1‑149 (MQ=255)
                    gTTTTTTTTCTTCGGCGTTTCCAGCGGATTACCCATCAGTAAGTTGTTTAGGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATCCTTACC‑TGGGGGG‑GG‑CGGGCCA‑GTCGCC‑TTAATTTCCCCCGCCAGCAAAAAGCAAc                                                                                                                                    <  2:2599285/150‑1 (MQ=255)
                        attatCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTTCCCCCCGCATGATGATGGGGGCAACGCTGATGCTTACC‑TTGTGGG‑GG‑CAGGGCA‑GTCGCC‑CTTATTTTTCCCCCCCGCAAAAAAAAACGATg                                                                                                                                >  2:2846017/1‑150 (MQ=255)
                         ttatTTTGGGCTTTCCCAGGGGATTACCCACCAGTAAGCTGTTTATGGCGGCCATTCCCCTTGGCATGATGATGGGCCCAACCCTGATGCTTCCC‑GGGTGGT‑GG‑CGGGCCA‑GTCGCT‑TTAATTTCCCCCGCCAGCAAAAAGCAACGATGc                                                                                                                               <  2:1226966/150‑1 (MQ=255)
                         ttatCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGGGTTTCCCCCGGCATGATGATGGGGGGCACGCCGATGCTTTCC‑CGGGGGG‑GG‑GGGGGCA‑GGGGCC‑TTAATTTTCCCCCCCCCCAAAAAACAAAGAGGc                                                                                                                               >  2:1215384/1‑150 (MQ=255)
                           atCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGTGTTGCCCCTCGCCTGGTGATTGGCGGAACTCCTATGATTAGC‑TCTTGGTGGG‑CAGGCC‑‑GTTGCC‑TTAATTTTCCACCCCCCCGAAAaacaacaaggccc                                                                                                                             >  2:3622241/1‑143 (MQ=255)
                           atCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTTCCCCCTGCCTGGTTATGGGGGCAACGCTGATGGTTTCC‑TGGGGGG‑GG‑GGGGGCC‑GGCGCC‑TTTATTTTTCCCCCCCGCAAAAAAGAACGATGCAg                                                                                                                             >  2:398508/1‑150 (MQ=255)
                             cTTCGGCGTTTCCGGCGGATTATCCATCAGTAAGCTGTTTAGGGCGGGCATTGCCCCTGGCATGATGAGGGGCGCAACGCTGATGCTTACC‑TGGGGGT‑GG‑CAGGCCA‑GTCGCC‑TTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGGa                                                                                                                           <  2:867755/150‑1 (MQ=255)
                                cGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTTCCCCCCGCCTGATGATGGGGGCAACGCTGGTGCTTTCC‑TGGTGGG‑GG‑GGGGCCC‑GGCGCC‑CTAATTTTCCCCCCCCGCAAAAAGCAAAGGAGCAGGgaaa                                                                                                                        >  2:639059/1‑149 (MQ=255)
                                  gCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCCTTTCCCCCCGCCTGATGATGGGGGCAAAGCTGATGCTTTCC‑TTGTGGT‑GG‑GAGGGCA‑GGCGCC‑CTTATTTTTCCCCCCCGCCAAAAACAAAGATGCAGGGAATCt                                                                                                                      >  2:136982/1‑150 (MQ=255)
                                        ccAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCCGGCCTGATGATGGGGGCAACGCTGATGCTTACC‑TGGTGGG‑GG‑GAGGGCA‑GGCGCC‑TTTATTTTCCCCCCCCCCCAAAAACAACGATGCAGGGAAACTGGGAct                                                                                                                >  2:529432/1‑150 (MQ=255)
                                          aGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGGATTGCCCCCGGCATGGTGGTGGGGGCGAAGCCGGTGCTTTCC‑TGGGGGG‑GG‑GAGGCCC‑GCCCCC‑CTAATTTTCCCCCCCCGCAAAAAACAACAAATCAGGAAAACTGGCActct                                                                                                              >  2:2087975/1‑150 (MQ=255)
                                          aGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTTCCCCCGGCGTTATGATGGGCGCAAAGCCGATGGTTACC‑TGGTGGG‑GG‑GCGGGCC‑GTCGCC‑CTATTTTACCCCCCCACCCAAAAACAAAGAAGGAGGGAATCTGGGActct                                                                                                              >  2:3434267/1‑150 (MQ=255)
                                              gATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACC‑TGGTGGT‑GG‑CAGGCCA‑GTCGCC‑TTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTg                                                                                                          <  2:3187528/150‑1 (MQ=255)
                                                ttATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCCGGCATGATGATGGGGGCAACGCTGATGCTTTCC‑TGGTGGT‑GG‑CAGGGCA‑GTCGCC‑TTAATTTACCCCCCCCGCAAAAAGCAACGATGCAGGGAATCTGGCACTCTTTTTtc                                                                                                        >  2:2152043/1‑150 (MQ=255)
                                                 tATCCATCAGTAAGCTGTTTATGGCGGGCAGTGCCCCCGGCCGGGTGATGGGGGCCACCCTGATGGTTACC‑CGGGGGG‑GG‑GAGGCCC‑GGCGCC‑CTTATTTTCCCCGCCCGCCAAAAACAACGATGGAGGGAATCAGGGACCTtttttttt                                                                                                       >  2:3492468/1‑146 (MQ=255)
                                                   tCCATCAGTAAGTTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCACCGCTGATGCTTCCC‑TGGGGGT‑GG‑CGGGCCG‑GTCGCT‑TTATTTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTcc                                                                                                     <  2:19152/150‑1 (MQ=255)
                                                    ccATCAGTAAGCTGTTTATGGCGGGCTTGGCCCCTGGCATGATGAGGGGCGCACCGCGGATGCTTCCC‑TGGTGGG‑GG‑CGGGCCA‑GTCGCT‑TTATTTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCg                                                                                                    <  2:3923166/150‑1 (MQ=255)
                                                     cATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACC‑TGGTGGT‑GG‑CAGGCCA‑GTCGCT‑TTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCgg                                                                                                   <  1:3551620/150‑1 (MQ=255)
                                                       acAGTAAGCTTTTTAGGGCGGGCTTTCCCCCTGGCATGATGATGGGCGAAACGCTGATGCTTCCC‑GGGTGGT‑GG‑CGGCCCA‑GTCCCC‑TTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTa                                                                                                 <  2:2008819/149‑1 (MQ=255)
                                                        cAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACC‑TGGTGGT‑GG‑CAGGCCA‑GTCGCC‑TTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTAt                                                                                                <  1:3369213/150‑1 (MQ=255)
                                                           tAAGCTGTTTATGGCGGGCATTTCCCCTGGCATGATGATGGGGGCAACGCTGATGCTTTCC‑TGGTGGG‑GG‑GAGGGCC‑GTCGCC‑TTTATTTATCCCGCCCGCAAAAAACAACGATGCAGGGAAACTGGCACTCTTTTTTTTCCGGTATCTg                                                                                             >  2:3913830/1‑150 (MQ=255)
                                                             aGCTGTTTATGGCGGGCATTGCCCCCGGCCTGATGTTGGGGGCCACGCTGATGCTTACC‑TTGTGGG‑GG‑GAGGGCA‑GTCGCC‑TTAATTTTCCCCCCCCCCCAAAAACAAAGAAGCAGGGAAAATGGGACTCTTTTTTTTCCCGTCTCTggg                                                                                           >  2:3106733/1‑150 (MQ=255)
                                                                tGTTTATGGCGGGCATTGCCCCCGGCATGATGATGGGGGCAAAGCTGATGGTTTCC‑TGGTGGG‑GG‑GCGGCCA‑GGCGCC‑TTAATTTTCCCCCCCAGCAAAAAACAACGAAGCAGGGAAACTGGGACTCTTTTTTTTCCGGTGTTTgggggg                                                                                        >  2:3564590/1‑147 (MQ=255)
                                                                 gTTTATGGCGGGCATTGCCCCCCGCATGATGATGGGGGCCACGCTGATGCTTTCC‑TGGTGGG‑GG‑GAGGCCC‑GTCGCC‑CTTATTTTCCCCCCCCGCAAAAAACAACCATGCAGGAAATATGGCACTCTTTTTTTTCCGGTATCTGGGCGCt                                                                                       >  2:3523185/1‑150 (MQ=255)
                                                                    tagggCGGGCATTGCCCTTGGAATGATGATGGGCGCAACGCTGATGCTTCCC‑TGGTGGT‑GG‑CAGGCCA‑GTCGCT‑TTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTAtt                                                                                    <  2:3037354/147‑1 (MQ=255)
                                                                     aTGGCGGGCATTGCCCCCGGCATGATGATTGGGGCAACGCTGATGCTTACC‑TGGTGGG‑GG‑CAGGCCC‑GTCGCC‑TTAATTTACCCCGCCAGCCAAAAGCAACGGTGCAGGAAATCTGGCACTCTTTTTTCTCCGGTATCTGGGCGCTGttt                                                                                   >  2:1398249/1‑150 (MQ=255)
                                                                     aTGGCGGGCATGGCCCCTGGCATGAGGATGGGCGCAGCGCTGTTGCTTGCC‑TGGTGGT‑GG‑CGGGCCA‑GTCGCC‑TTATTTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGttt                                                                                   <  2:2277663/150‑1 (MQ=255)
                                                                        gCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACC‑TGGTGGT‑GG‑CAGGCCA‑GTCGCC‑TTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTttctt                                                                                >  2:3848121/1‑150 (MQ=255)
                                                                          gggCTTTGCCCCGGGCATGTTGATGGGCCCAACGCTGATGCTTACC‑TGG‑GGT‑GGCCGGGCCA‑GTCGCT‑TTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTcc                                                                              <  2:358701/150‑1 (MQ=255)
                                                                           ggCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTCCC‑TGGTGGT‑GG‑CAGGCCA‑GTCGCC‑TTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCa                                                                             <  1:3191788/150‑1 (MQ=255)
                                                                            gCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACC‑TGGTGGT‑GG‑CAGGCCA‑GTCGCC‑TTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGTTATCTGGGCGCTGTTTCTTCCAg                                                                            >  1:3442570/1‑150 (MQ=255)
                                                                             cATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACC‑TGGTGGT‑GG‑CAGGCCA‑GTCGCC‑TTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGt                                                                           >  1:3494700/1‑150 (MQ=255)
                                                                               ttGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACC‑TGGTGGT‑GG‑CAGGCCA‑GTCGCC‑TTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGtca                                                                         >  2:1041701/1‑150 (MQ=255)
                                                                                tcccctTGGCATGATGAGGGGCGAAACGCTGAGGTTTCCC‑GGGTGGT‑GG‑C‑GGCCAGGTCTCT‑TTATTTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGtcat                                                                        <  2:592004/148‑1 (MQ=255)
                                                                                tGCCCCTGGCATGATGATAGGCGCAACGCTGATGCTTACC‑TGGTGGT‑GG‑CAGGCCA‑GTCGCC‑TTAATTTACCCCGCCAGCAAAAAACAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGtcat                                                                        >  1:2566805/1‑150 (MQ=255)
                                                                                 gCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACC‑TGGTGGT‑GG‑CAGGCCA‑GTCGCC‑TTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGtcatc                                                                       >  2:3221/1‑150 (MQ=255)
                                                                                  ccccTGGCATGATGATGGGCGCAACGCTGATGCTTACC‑TGGTGGT‑GG‑CAGGCCA‑GTCGCC‑TTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGtcatca                                                                      >  2:2665131/1‑150 (MQ=255)
                                                                                   cccTGGCATGATGATGGGCGCAACGCTGATGCTTACC‑TGGTGGT‑GG‑CAGGCCA‑GTCGCC‑TTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTcatcat                                                                     <  1:3650700/150‑1 (MQ=255)
                                                                                   cccTGGCATGATGATGGGCGCAACGCTGATGCTTACC‑TGGTGGT‑GG‑CAGGCCA‑GTCGCC‑TTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTcatcat                                                                     >  2:1658909/1‑150 (MQ=255)
                                                                                    ccTGGCATGATGATGGGCGCAACGCTGATGCTTACC‑TGGTGGT‑GG‑CAGGCCA‑GTCGCC‑TTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCatt                                                                    >  1:2020529/1‑150 (MQ=255)
                                                                                    ccTGGCATGATGATGGGCGCAACGCTGATGCTTACC‑TGGTGGT‑GG‑CAGGCCA‑GTCGCC‑TTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCatt                                                                    >  1:2523731/1‑150 (MQ=255)
                                                                                      tGGCATGATGATGGGCGCAACGCTGATGCTTACC‑TGGTGGT‑GG‑CAGGCCA‑GTCGCC‑TTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCattat                                                                  >  2:2169078/1‑150 (MQ=255)
                                                                                      tGGCATGATGATGGGCGCAACGCTGATGCTTACC‑TGGTGGT‑GG‑CAGGCCA‑GTCGCC‑TTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCattat                                                                  >  2:1886698/1‑150 (MQ=255)
                                                                                       ggCATGATGATGGGCGCAACGCTGATGCTTACC‑TGGTGGT‑GG‑CAGGCCA‑GTCGCC‑TTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCattatt                                                                 <  1:1620287/150‑1 (MQ=255)
                                                                                        gCATGATGATGGGCGCAACGCTGATGCTTACC‑TGGTGGT‑GG‑CAGGCCA‑GTCGCC‑TTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTg                                                                >  1:460781/1‑150 (MQ=255)
                                                                                         cATGATGATGGGCGCAACGCTGATGCTTACC‑TGGTGGT‑GG‑CAGGCCA‑GTCGCC‑TTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTgg                                                               >  1:2925956/1‑150 (MQ=255)
                                                                                          atgatgatgGGCGCAACGCTGATGCTTACC‑TGGTGGT‑GG‑CAGGCCA‑GTCGCC‑TTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGc                                                              >  1:1330529/1‑150 (MQ=255)
                                                                                           tgatgatgGGCGCAACGCTGATGCTTACC‑TGGTGGT‑GG‑CAGGCCA‑GTCGCC‑TTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCg                                                             >  2:2851353/1‑150 (MQ=255)
                                                                                           tgatgatgGGCGCAACGCTGATGCTTACC‑TGGTGGT‑GG‑CAGGCCA‑GTCGCC‑TTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGATATCTGGGCGCTGTTTTTTCCAGTCATCATTATTGGCg                                                             >  2:3345759/1‑150 (MQ=255)
                                                                                           tgatgaggGGCGCAACGCTGATGCTTACC‑TGGTGGT‑GG‑CAGGCCA‑GTCGCC‑TTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCg                                                             <  1:3462144/150‑1 (MQ=255)
                                                                                             atgatgGGCGCAACGCTGATGCTTACC‑TGGTGGT‑GG‑CAGGCCA‑GTCGCC‑TTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCggg                                                           >  2:73417/1‑150 (MQ=255)
                                                                                                 ggggCGCAGCGCTGATGCTTACC‑GGGGGGT‑GG‑CAGCCCA‑TTCGCA‑TTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTc                                                       <  2:3697602/149‑1 (MQ=255)
                                                                                                  gggCGCAACGCTGATGCTTACC‑TGGTGGT‑GG‑CAGGCCA‑GTCGCC‑TTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGttttg                                                      >  2:4067103/1‑148 (MQ=255)
                                                                                                  gggCGCAACGCTGATGCTTACC‑TGGTGGT‑GG‑CAGGCCA‑GTCGCC‑TTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGttttg                                                      >  2:2814636/1‑148 (MQ=255)
                                                                                                  gggCGCAACGCTGATGCTTACC‑TGGTGGT‑GG‑CAGGCCA‑GTCGCC‑TTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGgtttg                                                      >  1:1844262/1‑148 (MQ=255)
                                                                                                      gcACCGCTGATGCTTCCC‑TGGTGGT‑GG‑CGGGCAA‑GTCGCT‑TTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTcc                                                  <  2:1815697/150‑1 (MQ=255)
                                                                                                      gcAACGCTGATGCTTACC‑TGGTGGT‑GG‑CAGGCCA‑GTCGCC‑TTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTTGCTcc                                                  >  1:2612322/1‑150 (MQ=255)
                                                                                                       cAACGCTGATGCTTACC‑TGGTGGT‑GG‑CAGGCCA‑GTCGCC‑TTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTTCTCCg                                                 >  1:1434659/1‑150 (MQ=255)
                                                                                                       cAACGCTGATGCTTACC‑TGGTGGT‑GG‑CAGGCCA‑GTCGCC‑TTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTCGCTccc                                                 >  1:2213031/1‑149 (MQ=255)
                                                                                                        aaCGCTGATGCTTACC‑TGGTGGT‑GG‑CAGGCCA‑GTCGCC‑TTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCgg                                                <  1:101881/150‑1 (MQ=255)
                                                                                                         aCGCTGATGCTTACC‑TGGTGGT‑GG‑CAGGCCA‑GTCGCC‑TTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTTGCTCCgcc                                               >  2:1681551/1‑148 (MQ=255)
                                                                                                            cTGATGCTTACC‑TGGTGGT‑GG‑CAGTCCA‑GTCGCC‑TTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTg                                            >  1:2357344/1‑150 (MQ=255)
                                                                                                            cTGATGCTTACC‑TGGTGGT‑GG‑CAGGCCA‑GTCGCC‑TTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTTGCTCCGGcccg                                            >  1:1946356/1‑148 (MQ=255)
                                                                                                             tGAGGCTTTCC‑TGGTGGG‑GG‑GAGGCAT‑GTCCCT‑TTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGt                                           <  1:1513918/150‑1 (MQ=255)
                                                                                                               aTGCTTACC‑TGGTGGT‑GG‑CAGGCCA‑GTCGCC‑TTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGttt                                         >  2:1948825/1‑150 (MQ=255)
                                                                                                               aTGCTTACC‑TGGTGGT‑GG‑CAGGCCA‑GTCGCC‑TTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCCGttt                                         >  1:1073097/1‑150 (MQ=255)
                                                                                                                ttcTTACC‑TGGGGGG‑GG‑CAGGCCA‑GCCGCC‑TTATTTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTa                                        <  2:1244711/148‑1 (MQ=255)
                                                                                                                tGCTTCCC‑TGGGGGT‑GG‑CAGGCCA‑GTCGCC‑TTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTa                                        <  1:835059/150‑1 (MQ=255)
                                                                                                                 gtttccc‑TGGTGGG‑GG‑CGGACAA‑GGGGCC‑TTAATTTTCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTAc                                       <  1:3924717/145‑1 (MQ=255)
                                                                                                                 gCTTACC‑TGGTGGT‑GG‑CAGGCCA‑GTCGCC‑TTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTGCTCCCGCCCGttttt                                       >  1:2071666/1‑148 (MQ=255)
                                                                                                                     aCC‑TGGTGGT‑GG‑CAGGCCA‑GTCGCC‑TTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTTTCTCCCGCCCGtttttcccc                                   >  1:1276604/1‑144 (MQ=255)
                                                                                                                     aCC‑TGGTGGT‑GG‑CAGGCCA‑GTCGCC‑TTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCTCTTCCGCCTGtttttcccc                                   >  2:1541952/1‑144 (MQ=255)
                                                                                                                      cc‑TGGTGGT‑GG‑CAGGCCA‑GTCGCC‑TTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTGCTCCCGCCCGTTTTccccca                                  >  1:2763965/1‑148 (MQ=255)
                                                                                                                       c‑TGGTGGT‑GG‑CAGGCCA‑GTCGCC‑TTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTAt                                                                                                <  1:1634597/87‑1 (MQ=255)
                                                                                                                         tggtggt‑gg‑CAGGCCA‑GTCGCC‑TTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTGCTCCGGCCCGTTTTCCCCC‑Acc                               >  2:1715854/1‑150 (MQ=255)
                                                                                                                                 gg‑CAGGCCA‑GTCGCC‑TTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGCTTTTCTCCCGCCTCTTTTCCCCT‑TCCCAAGccc                        >  2:2644922/1‑149 (MQ=255)
                                                                                                                                     aGGCCA‑GTCGCC‑TTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTGCTCCCGCCTGTTTTTCCCC‑ACCCAAGccccgg                     >  2:1871028/1‑146 (MQ=255)
                                                                                                                                       gCCA‑GTCGCT‑TTATTTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCT‑ACCGAAGCCGGGGcc                   <  2:4129075/150‑1 (MQ=255)
                                                                                                                                       gCCA‑GTCGCC‑TTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCACTCATCATTATTGGCGGGTTTTTCTCCCGGCCGTTTTCTCCT‑ACCCAAACCaggggc                   >  2:708569/1‑148 (MQ=255)
                                                                                                                                       cccA‑GTCCCT‑TTATTTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCT‑ACCGAAGCCGGGGcc                   <  2:4197453/149‑1 (MQ=255)
                                                                                                                                           agccccc‑TTATTTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCT‑ACCGAAGCCGGGGCCGtt                <  1:117968/145‑1 (MQ=255)
                                                                                                                                            gTCGCC‑TTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTTCTCCGGCCCCTTTTCCCCTCCCCCAACCCGGGGcgctg                >  2:9167/1‑146 (MQ=255)
                                                                                                                                                 c‑TTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCT‑ACCGAAGCCGGGGCCGTTgctgct          <  1:639402/150‑1 (MQ=255)
                                                                                                                                                 c‑TTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTGCTCCCGCCTGTTTTCCCCC‑ACCCAAGCCCGGGCCGTTgctgct          >  1:906154/1‑150 (MQ=255)
                                                                                                                                                     aaTTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTTGCTCCCGCCTGTTTTTCCCT‑CCCCCAACCGGGGCCGTTGCTGCTTTc       >  2:1567557/1‑150 (MQ=255)
                                                                                                                                                      aTTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCT‑ACCGAAGCCGGGGCCGTTGCTGCTTTCt      <  1:1718237/150‑1 (MQ=255)
                                                                                                                                                      aTTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCT‑ACCGAAGCCGGGGCCGTTGCTGCTTTCt      <  1:2421878/150‑1 (MQ=255)
                                                                                                                                                          aCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTGCTCCGGCCCGTTTACCCCC‑ACCCAAGCCGGGGCCGGTGCTGCTTTCttttc  >  1:4065105/1‑146 (MQ=255)
                                                                                                                                                          aCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCAATCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGATCCGGCCTGTTTACCCCT‑ACCGAAGCCGGGGCAGTTGCTGCTTTCTATgc  <  2:1853982/150‑1 (MQ=255)
                                                                                                                                                          |                                                                                                                                                      
ATTATTCCCCCTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACC‑TGGTGGT‑GG‑CAGGCCA‑GTCGCC‑TTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCT‑ACCGAAGCCGGGGCCGTTGCTGCTTTCTATGC  >  AP012306/3182712‑3183010

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑