Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123063,324,4330TA24.4% 535.3 / 92.8 234intergenic (+2/+52)yieF/yieGchromate reductase, Class I, flavoprotein/predicted inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (132/45);  new base A (0/57);  total (132/102)
Fisher's exact test for biased strand distribution p-value = 1.24e-26
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 276 total aligned reads displayed.

GGTGATGAACAAGCCGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGG‑AGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGA‑CCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGATTTTCA  >  AP012306/3324284‑3324582
                                                                                                                                                       |                                                                                                                                                     
ggTGATGAACAAGCCGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGG‑AGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGA‑CCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCtaaat                                                                                                                                                       <  1:3311132/150‑1 (MQ=255)
ggTGATGAACAAGCCGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGG‑AGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGA‑CCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCtaaat                                                                                                                                                       <  1:576936/150‑1 (MQ=255)
ggTGATGAACAAGCCGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGG‑AGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGA‑CCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCtaaat                                                                                                                                                       <  2:1754687/150‑1 (MQ=255)
  tgatgaACAAGCCGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGG‑AGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGA‑CCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCtaaataa                                                                                                                                                     >  2:644978/1‑150 (MQ=255)
      gaACAAGCCGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGG‑AGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGA‑CCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATaaaaaa                                                                                                                                                 <  2:3801721/150‑1 (MQ=255)
        aCAAGCCGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGG‑AGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGA‑CCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAAcc                                                                                                                                               <  1:302202/150‑1 (MQ=255)
            gCCGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGG‑AGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGA‑CCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGcc                                                                                                                                           >  1:1327532/1‑150 (MQ=255)
             cgggAATTTAGGGGGGGGGTGATTCAGAACAAAGTGGATCCGCAAACCGG‑AGAGGTGATTGATCAGGGTACGCTGGACCCCCTGACCGGGAAATTGC‑CCGCTTTTGGTGATTTTATTCAGGGATTTAAGATCAAAAAAAAAAACCCGCCa                                                                                                                                          <  2:4158105/148‑1 (MQ=255)
              cGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGG‑AGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGA‑CCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAg                                                                                                                                         <  1:2180305/150‑1 (MQ=255)
               ggAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGG‑AGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGA‑CCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGc                                                                                                                                        >  2:4123393/1‑150 (MQ=255)
                  aTTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGG‑AGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGA‑CCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAAt                                                                                                                                     >  1:2545842/1‑150 (MQ=255)
                  aTTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGG‑AGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGA‑CCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAAt                                                                                                                                     >  1:3737176/1‑150 (MQ=255)
                   tttATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGG‑AGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGA‑CCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATc                                                                                                                                    >  2:897433/1‑150 (MQ=255)
                     taggggCGGCGTGATTCAGAACAAAGTTGACCCGCAAACGGG‑AGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGAAATTGA‑CCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAAAAAAAACCCCGCCAGCAATcat                                                                                                                                  <  2:14008/147‑1 (MQ=255)
                       tGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGG‑AGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGA‑CCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATcatgc                                                                                                                                >  1:1455752/1‑150 (MQ=255)
                         ggcgggGTGATTAAGAAAAAAGTTGACCCG‑AAACCGGAAGAGGTGTTTGATGGGGTTACGCTGCACCCCCTGACGGGGAAATTAA‑CCGTTTTGGGGGATTTTATTCGGGGATTAAAAATTAAAAAAAAAACCCCGCCAGCAATcatgcat                                                                                                                              <  2:2031513/150‑1 (MQ=255)
                             gcgTGATTCAGAACAAAGTTGATCCGCAAACCGG‑AGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGA‑CCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAAAAAAAAACCCGCCAGCAATCATGCATGGCg                                                                                                                          <  1:2159216/150‑1 (MQ=255)
                               gTGATTCAGAACAAATTTGACCCGCAAACGGG‑AGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGC‑CCGCTTTTGGTGAGTTTATTCAGGGAGTTAAGATCAAAAAAAAAACCCCGCCAGCAATCATGCATGGCggg                                                                                                                        <  2:837195/150‑1 (MQ=255)
                               gTGATTCAGAACAAAGTTGATCCGCAAACCGG‑AGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGA‑CCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCggg                                                                                                                        >  1:1895381/1‑150 (MQ=255)
                                tGATTCAGAACAAAGTTGATCCGCAAACCGG‑AGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGA‑CCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGt                                                                                                                       >  1:653242/1‑150 (MQ=255)
                                tGATTCAGAAAAAATTTGACCCGCAAACGGA‑AAAAGTGATTGATGGGGGAACGGGGAACCGCCGGACGGGGCAATTGC‑CCTCATTTGGTGGGTTTATCAGGCGAGTTAAGATATAAATAAAAACCCCGCCAGCAATCATGCATGGCGGGt                                                                                                                       <  2:2512282/150‑1 (MQ=255)
                                           aaaGTTGATCCGCAAACCGG‑AGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGA‑CCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGGTTTTAACgcgc                                                                                                            >  1:2211145/1‑150 (MQ=255)
                                            aaGTTGATCCGCAAACCGG‑AGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGA‑CCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCt                                                                                                           >  1:2349562/1‑150 (MQ=255)
                                               ttGATCCGCAAACCGG‑AGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGA‑CCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATc                                                                                                        >  1:3572601/1‑150 (MQ=255)
                                                tGATCCGCAAACCGG‑AGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGA‑CCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCAAAAAAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCg                                                                                                       <  1:148258/150‑1 (MQ=255)
                                                  acccGCAAACCGG‑AGAAGTGATTGATCGGGGTACGCTGGACCCCCTGACCGGGCAATTGA‑CCGCTTTTGGTGATTTTATTCAGCGAGTTAAGATCAAAAAAAAAACCCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGAt                                                                                                     <  2:774767/148‑1 (MQ=255)
                                                  aTCCGCAAACCGG‑AGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGA‑CCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGGTTTTAACCCCCTATCGAt                                                                                                     >  2:3569692/1‑150 (MQ=255)
                                                    ccGCAAACCGG‑AGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGA‑CCGCATTTGGTGATTTTATTCAGCGAGTTAAGATCTAAAAAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGAttt                                                                                                   <  1:2530410/150‑1 (MQ=255)
                                                      gCAAACCGG‑AGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGA‑CCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTa                                                                                                 >  2:1624273/1‑150 (MQ=255)
                                                           ccGG‑GGAAGTGATTGATCAGGGTACGCTGGACCACCTGCCCGGGCAATTGC‑CCGCTTTTGGTGTGTTTATTAAGGGAGTTAAGATCTAAATAAAAACCCCGCCAGCAATCATGCATGGCGGG‑TTTTAACGCGCTATCGATTTTAGTGAGc                                                                                           <  2:457495/150‑1 (MQ=255)
                                                           ccGG‑AGAAGTGATTGATGAGGGTACGCTGGCCCACCTGACCGGGCAATTGA‑CCGCTTTTGGTGAGTTTATTCAGGGAGTTAAGATATAAATAAAAACCCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAg                                                                                            <  2:264911/150‑1 (MQ=255)
                                                            cGG‑AGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGA‑CCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGc                                                                                           >  1:2753666/1‑150 (MQ=255)
                                                                    gTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGA‑CCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATa                                                                                    >  1:3906159/1‑150 (MQ=255)
                                                                        ttgatCAGGGTACGCTGGACCACCTGACCGGGCAATTGA‑CCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGa                                                                                >  1:1776679/1‑150 (MQ=255)
                                                                          gatCAGGGTACGCTGGACCACCTGACCGGGAAATTGA‑CCGTATTTGGTGAGTTTATTCAGCGAGTAAAGATAAAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACa                                                                              <  2:1483896/150‑1 (MQ=255)
                                                                              aGGGTACGCTGGACCACCTGACCGGGCAATTGA‑CCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGGTAAAGACAATCt                                                                          >  2:3322881/1‑150 (MQ=255)
                                                                               ggttACGTTGGCCCACCGGCCCGGGCAATTGC‑CCGCATTTGGTGATTTTATTCAGCGAGTTAAGATCTAAAAAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCtt                                                                         <  2:1295298/147‑1 (MQ=255)
                                                                               gggTACGCTGGACCCCCTGACCGGGAAATTGA‑CCGCATTTGGTGAGTTTATTCAGCGAGTTAAGAGCTAAAAAAAAAACCCTCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATGAAGACAATCtt                                                                         <  2:3577714/150‑1 (MQ=255)
                                                                                 gTACGTTGGACCACCTGACCGGGAAATTGA‑CCGTATTTGTTCAGTTTATCCAGCGAGTTAAGAATTAAAAAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCa                                                                       <  2:1093215/150‑1 (MQ=255)
                                                                                 gTACGCTGGACCACCTGACCGGGCAATTGA‑CCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCa                                                                       >  2:2774226/1‑150 (MQ=255)
                                                                                   aCGCTGGACCCCCTGACGGGAAAATTGA‑CCGCTTTTGGTGATTTTATTCAGCGATTTAAGATCAAAAAAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAgg                                                                     <  2:747809/150‑1 (MQ=255)
                                                                                   aCGCTGGACCACCTGACGGGGCAATTGA‑CCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAgg                                                                     <  1:2416396/150‑1 (MQ=255)
                                                                                       tGGACCACCTGACCGGGCAATTGA‑CCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGGTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAAACAATCTTCAGGATaa                                                                 >  2:2126953/1‑150 (MQ=255)
                                                                                           cccccTGACGGGGAAATTGC‑CCGCTTTTGGTGATTTTATTCAGCGAGTTAAGATCAAAAAAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAAcag                                                             <  2:3083993/147‑1 (MQ=255)
                                                                                            caccTGACCGGGCAATTGA‑CCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAAGATAAAcagc                                                            >  1:46152/1‑150 (MQ=255)
                                                                                             accTGACCGGGCAATTGA‑CCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAAcagca                                                           >  1:3156961/1‑150 (MQ=255)
                                                                                              ccTGACCGGGCAATTGA‑CCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAAcagcag                                                          <  1:349215/150‑1 (MQ=255)
                                                                                              ccTGACCGGGCAATTGA‑CCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGGTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAAcagcag                                                          >  2:1945825/1‑150 (MQ=255)
                                                                                               ctgccggggAAATTGA‑CCGCTTTTGGTGATTTTATTCGGCGAGTTAAGATATAAAAAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACagcagc                                                         <  2:3456094/144‑1 (MQ=255)
                                                                                                tgcccGGGAAATTGA‑CCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCAAAAAAAAAACCCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAgcg                                                        <  1:170403/147‑1 (MQ=255)
                                                                                                tGACGGGGCAATTGC‑CCGCTTTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAAAAAAAACCCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAgcg                                                        <  2:538187/150‑1 (MQ=255)
                                                                                                   ccGGGGAATTTACCCGCATTGGGGGTGTTTATACAGCGAGAAAAGAACAAAAAAAAACCCCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCa                                                      <  2:4257049/150‑1 (MQ=255)
                                                                                                    cGGGCAATTGA‑CCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAACAGCGCAAc                                                    >  2:720192/1‑150 (MQ=255)
                                                                                                        cAATTGC‑CCGCTTTTGGTGAGTTTATCCAGCGAGTAAAGATCAAAATAAAAACCCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACgatg                                                <  1:2028401/150‑1 (MQ=255)
                                                                                                        aaaTTGA‑CCGTATTGGGTGAGTTTATTCAGGGATTAAAGATCAAAATAAAAACCCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACgatg                                                <  2:1846214/149‑1 (MQ=255)
                                                                                                        aaaTTGA‑CCGAATTTGGTGAGTTTATTCAGGGAGTTAAGATCAAAAAAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACgatg                                                <  1:288248/149‑1 (MQ=255)
                                                                                                         aaTTGA‑CCGCTTTTGGTGATTTTATTCAGCGAGTTAAGATCTAAATAAAAACCCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACgatga                                               <  2:2394191/150‑1 (MQ=255)
                                                                                                          aTTGA‑CCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCAAAATAAAAACCCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACgatgat                                              <  1:4024915/150‑1 (MQ=255)
                                                                                                           ttGA‑CCGCTTTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAAAAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGAtt                                             <  1:4103469/150‑1 (MQ=255)
                                                                                                           ttGA‑CCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGAtt                                             <  1:2847513/150‑1 (MQ=255)
                                                                                                            tGA‑CCGCATTTGGTGATTTTATTCAGCGAGTTAAGATCAAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTa                                            <  2:2824498/150‑1 (MQ=255)
                                                                                                             gA‑CCGCATTTGGTAATTTTATTTAGCCGGTTAAGAACTAAAAAAAAACCCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTAc                                           <  2:498344/150‑1 (MQ=255)
                                                                                                                ccGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGc                                         >  2:2571704/1‑150 (MQ=255)
                                                                                                                 cGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCa                                        >  1:1215838/1‑150 (MQ=255)
                                                                                                                 cGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAAACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCa                                        >  1:4217094/1‑150 (MQ=255)
                                                                                                                  gCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTTAGGATAAACAGCAGCGCAACGATGATTACGCAc                                       >  1:1566059/1‑150 (MQ=255)
                                                                                                                    ttttGGTGATTTTATTCAGCGAGTTAAGATCTAAAAAAAAACCCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACgg                                     <  2:4045094/149‑1 (MQ=255)
                                                                                                                    aTTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACgg                                     >  1:1614526/1‑150 (MQ=255)
                                                                                                                        ggTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCtt                                 >  2:3218514/1‑150 (MQ=255)
                                                                                                                        ggTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCtt                                 >  1:3680761/1‑150 (MQ=255)
                                                                                                                           gAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGGTTTTTACGGGGCATCGGTTTTAAAGAGCGTCGCTAAAGAAAATGTTTAGGGTAAACAGCCGCGCAACGATGGTTACGCCCGGGCTTAGa                              >  2:2257775/1‑150 (MQ=255)
                                                                                                                           gAGTTAATTAAGCGAGTTAAAATCAAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGa                              <  2:1150939/150‑1 (MQ=255)
                                                                                                                            attttATTCAGCGATTAAAGATAAAAAAAAAAACCCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGAt                             <  2:3173970/148‑1 (MQ=255)
                                                                                                                             gTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATc                            >  1:1081979/1‑150 (MQ=255)
                                                                                                                              tttATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCa                           >  2:1539765/1‑150 (MQ=255)
                                                                                                                               ttattaAGGGAGTTAGGATCAAAAAAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCAc                          <  1:399123/150‑1 (MQ=255)
                                                                                                                                tATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACg                         >  1:977988/1‑150 (MQ=255)
                                                                                                                                     agggAGTTAAGATCTAAAAAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGAc                    <  2:328146/147‑1 (MQ=255)
                                                                                                                                     aGCGAGTTAAGTCATAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGAc                    <  1:2627146/150‑1 (MQ=255)
                                                                                                                                     aGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGGTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGAc                    >  2:3066113/1‑150 (MQ=255)
                                                                                                                                     aGCGAGTTAAGATATAAATAAAAACCCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGAc                    <  2:2325065/150‑1 (MQ=255)
                                                                                                                                     aGCGAGTAAAGATATAAAAAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGAc                    <  1:3625124/150‑1 (MQ=255)
                                                                                                                                      gCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACg                   >  2:3609463/1‑150 (MQ=255)
                                                                                                                                      gCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACg                   >  1:3955862/1‑150 (MQ=255)
                                                                                                                                         aGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTcc                <  1:11121/150‑1 (MQ=255)
                                                                                                                                         aGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCAAGGGCTTAGATCACGGCGGCGTcc                >  2:1436835/1‑150 (MQ=255)
                                                                                                                                          gTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCg               >  1:192414/1‑150 (MQ=255)
                                                                                                                                           ttAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCgg              >  1:1290200/1‑150 (MQ=255)
                                                                                                                                           taaaGATCAAAATAAAAACCCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCgg              <  1:1001994/148‑1 (MQ=255)
                                                                                                                                            tAAGATCAAAAAAAATACCCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGt             <  2:3501541/150‑1 (MQ=255)
                                                                                                                                            aaaGATCTAAATAAAAACCCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGt             <  1:2095272/149‑1 (MQ=255)
                                                                                                                                               gATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTTACGCCCTTTCGAGTTTAGTGAGCGACGATAAAGACAATATTTCAGATAAACAGCAGCCCAACGGTGCATAAGCCCGGGCTTTGATCACCCAGACGTCCCGTAcc          >  2:2930522/1‑150 (MQ=255)
                                                                                                                                               gATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTAcc          >  1:838419/1‑150 (MQ=255)
                                                                                                                                               gATCAAAAAAAAAACCCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTAcc          <  2:1976045/150‑1 (MQ=255)
                                                                                                                                                 tCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGa        >  1:735830/1‑150 (MQ=255)
                                                                                                                                                  cTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGAt       >  1:544666/1‑150 (MQ=255)
                                                                                                                                                     aataaaAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGAtttt    >  1:3441538/1‑150 (MQ=255)
                                                                                                                                                      ataaaAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGATTTtc   >  1:3278536/1‑150 (MQ=255)
                                                                                                                                                      ataaaAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGATTTtc   >  2:1726564/1‑150 (MQ=255)
                                                                                                                                                       taaaAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGATTTtca  >  1:469801/1‑150 (MQ=255)
                                                                                                                                                       |                                                                                                                                                     
GGTGATGAACAAGCCGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGG‑AGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGA‑CCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGATTTTCA  >  AP012306/3324284‑3324582

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑