Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123063,527,3680AC29.1% 558.6 / 51.7 237L140F (TTA→TTC) rhaSDNA‑binding transcriptional activator, L‑rhamnose‑binding
Reads supporting (aligned to +/- strand):  ref base A (145/23);  new base C (0/69);  total (145/92)
Fisher's exact test for biased strand distribution p-value = 4.08e-40
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 276 total aligned reads displayed.

CGATTTCAGTTTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCC‑TCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAGGTGAATTG  >  AP012306/3527219‑3527517
                                                                                                                                                     |                                                                                                                                                      
cGATTTCAGTTTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTa                                                                                                                                                        >  1:2756059/1‑150 (MQ=255)
 gATTTCAGTTTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTAc                                                                                                                                                       >  1:3786342/1‑150 (MQ=255)
  aTTTCAGTTTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGCAGGGGAAAATGATTTagc                                                                                                                                                      >  1:573511/1‑148 (MQ=255)
      cAGTTTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCC‑TCg                                                                                                                                                 <  1:1005967/150‑1 (MQ=255)
         tttCTCGCCGGGCTGAATCAGTTTCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCC‑TCGAcc                                                                                                                                              <  1:1564762/150‑1 (MQ=255)
          ttCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCC‑TCGACCg                                                                                                                                             <  2:3409361/150‑1 (MQ=255)
           tctcGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCC‑TCGACCGc                                                                                                                                            >  2:194042/1‑150 (MQ=255)
              cGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCC‑TCGACCGCCAg                                                                                                                                         <  2:4157333/150‑1 (MQ=255)
               gCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCC‑TCGACCGCCAGt                                                                                                                                        >  1:326500/1‑150 (MQ=255)
                 cGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCC‑TCGACCGCCAGTcg                                                                                                                                      >  2:2328956/1‑150 (MQ=255)
                    gCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCC‑TCGACCGCCAGTCGCga                                                                                                                                   >  1:794965/1‑150 (MQ=255)
                         atcgtttGCTGCCACAAGAGCTGGAGGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAGGGGAAAAATGATTTCCCC‑TCGACCGCCAGTCGCGAGATCt                                                                                                                              <  2:446523/145‑1 (MQ=255)
                           cggTTGCTGCCACAAGGGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGAAGGAAGGGGAAAATGATTTCCCC‑TCGACCGCCAGTCGCGAGATCTTg                                                                                                                            <  2:142899/148‑1 (MQ=255)
                           cggTTGCTCCCAAAGGGGCTGGAGGGGCAGTATCCGTCTCGCGGGCGCGTTACCCACAGGTTTTGGCGGCGGGGGCGACGGCTGGTTGCACAGAGGAAACAGCAGGAGGGGAAAAATGTTTTCCCC‑TCGACCGCCAGTCGCGAGATCTTg                                                                                                                            <  2:3153922/148‑1 (MQ=255)
                            aGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCC‑TCGACCGCCAGTCGCGAGATCTTGt                                                                                                                           >  2:1757880/1‑150 (MQ=255)
                             gTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCC‑TCGACCGCCAGTCGCGAGATCTTGtt                                                                                                                          >  1:2861128/1‑150 (MQ=255)
                                 ctgcCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTCCCC‑TCGACCGCCAGTCGCGAGATCTTGTTTATg                                                                                                                      <  1:1199114/150‑1 (MQ=255)
                                 ctgcCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCC‑TCGACCGCCAGTCGCGAGATCTTGTTTATg                                                                                                                      >  2:1377342/1‑150 (MQ=255)
                                  tgcCACAAGGGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAGGGGGAAAATGATTTCCCC‑TCGACCGCCAGTCGCGAGATCTTGTTTATGc                                                                                                                     <  2:2127074/150‑1 (MQ=255)
                                   gcCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCC‑TCGACCGCCAGTCGCGAGATCTTGTTTATGCa                                                                                                                    >  1:2162803/1‑150 (MQ=255)
                                    ccACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCC‑TCGACCGCCAGTCGCGAGATCTTGTTTATGCaa                                                                                                                   >  2:2962112/1‑150 (MQ=255)
                                     cacaAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCC‑TCGACCGCCAGTCGCGAGATCTTGTTTATGCAAt                                                                                                                  >  2:3283142/1‑150 (MQ=255)
                                          gagCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCC‑TCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTg                                                                                                             >  2:3552863/1‑150 (MQ=255)
                                          gagCTGGATGGGCAGTATCCGTCTCACGGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGAGGGAACAGCAGGAAGGGAAAAATGATTTCCCC‑CCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTg                                                                                                             <  2:536972/150‑1 (MQ=255)
                                            gcgggATGGGCAGTATCCGTCTCACGGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTCCAAAGAGGGAACAGCAGGAGGGGAAAAATTATTTCCCC‑CCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGct                                                                                                           <  2:2311754/147‑1 (MQ=255)
                                             cTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCC‑TCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGctc                                                                                                          >  2:2363528/1‑150 (MQ=255)
                                              tGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCC‑TCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGctct                                                                                                         >  2:287332/1‑150 (MQ=255)
                                                gATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCC‑TCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTtg                                                                                                       >  1:4254418/1‑150 (MQ=255)
                                                gATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGAAAAATGTTTTCCCC‑TCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTtg                                                                                                       <  2:1798771/150‑1 (MQ=255)
                                                 aTGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTCCCC‑TCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTtgc                                                                                                      <  1:823678/150‑1 (MQ=255)
                                                    ggCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCC‑TCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTtgctgc                                                                                                   >  1:3280626/1‑150 (MQ=255)
                                                      cAGTATCCGTCTCACTGGCGCGTTAACCACGGCGTATTGCAGCAGGGGCGACAGCTGGTTGCACAGAAGGAACAGCAGGAGGGAGAAAATGATTTCCCC‑CCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGt                                                                                                 <  2:3981544/150‑1 (MQ=255)
                                                      cAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCC‑TCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGt                                                                                                 >  2:4217114/1‑150 (MQ=255)
                                                        gTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCC‑TCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTaa                                                                                               >  1:3140833/1‑150 (MQ=255)
                                                        gTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCC‑TCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTaa                                                                                               >  1:2824706/1‑150 (MQ=255)
                                                         tATCCGTCTCACTGGCGCTTTAACCACAGCGTATGGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTCCCC‑TCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTaaa                                                                                              <  1:2963817/150‑1 (MQ=255)
                                                         tATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCC‑TCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTaaa                                                                                              >  1:3695025/1‑150 (MQ=255)
                                                         tATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCC‑TCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTaaa                                                                                              >  1:1635014/1‑150 (MQ=255)
                                                           tCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCC‑TCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACAGCTCTTGCTGCGTAAAAg                                                                                            >  2:1324099/1‑150 (MQ=255)
                                                              gTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCC‑TCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAg                                                                                         >  1:3463605/1‑150 (MQ=255)
                                                                ctcACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCC‑TCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGtt                                                                                       >  2:366694/1‑150 (MQ=255)
                                                                  cACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTCCCC‑TCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTg                                                                                     <  1:3355487/150‑1 (MQ=255)
                                                                     gggCGCGTAAACCACAGGGTATGGCAGCAGGTGCGACAGCTGGTTGCAAAGAGGAAACAGCGGGAAGGGAAAAATGATTTCCCC‑CCGCCCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAg                                                                                  <  2:2089573/149‑1 (MQ=255)
                                                                           gTTACCCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTCCACAGATGGAACAGCAGAAAGGGGAAAATGATTTCCCC‑TCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAAc                                                                            <  1:2745327/150‑1 (MQ=255)
                                                                           gTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCC‑TCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAAc                                                                            >  1:735916/1‑150 (MQ=255)
                                                                             tAACCACAGCGTATTGCAGCAGGTGCGACAGTTGGTTGCACAGAGGGAACAGCAGGAAGGGGAAAATGATTTCCCC‑TCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCt                                                                          <  1:1826377/150‑1 (MQ=255)
                                                                               aCCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGAAAAATGATTTCCCC‑TCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTgg                                                                        <  2:2254218/150‑1 (MQ=255)
                                                                                  acaGCGTATTGCAACAGGGGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGAAAAATGTTTTCCCC‑CCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGaaa                                                                     <  2:3307851/150‑1 (MQ=255)
                                                                                   caGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCC‑TCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGaaaa                                                                    >  2:1218892/1‑150 (MQ=255)
                                                                                    aGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCC‑TCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAAc                                                                   >  2:2711609/1‑150 (MQ=255)
                                                                                      cGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCC‑TCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAg                                                                 <  1:364889/150‑1 (MQ=255)
                                                                                      cGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCC‑TCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAg                                                                 <  1:385328/150‑1 (MQ=255)
                                                                                       ttATTGCAGCAGGTGCAACAGCTGGTTGAAAAAAAGAAACAGAAGGAAGGGAAAAATAATTTCCCC‑TCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAgc                                                                <  2:713367/149‑1 (MQ=255)
                                                                                           tGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTCCCC‑TCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCAt                                                            <  1:1241942/150‑1 (MQ=255)
                                                                                            gcagcaGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCC‑TCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATc                                                           >  1:3546623/1‑150 (MQ=255)
                                                                                             cagcagGTGCGACAGCTGGTGGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTCCCC‑TCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCa                                                          <  1:624408/150‑1 (MQ=255)
                                                                                              agcagGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCC‑TCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCAc                                                         >  1:777888/1‑150 (MQ=255)
                                                                                              agcagGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCC‑TCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCAc                                                         >  1:3738267/1‑150 (MQ=255)
                                                                                                cagGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGAAAAATGTTTTCCCC‑TCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGt                                                       <  2:3211532/150‑1 (MQ=255)
                                                                                                 gggTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGAAAAATGTTTTCCCC‑TCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGtc                                                      <  2:3722439/149‑1 (MQ=255)
                                                                                                     gCGACAGCTGGTGGCACAGATGGAACAGCAGGAGGGGGAAAATGATTTCCCC‑TCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCaa                                                  <  1:971350/150‑1 (MQ=255)
                                                                                                      cGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCC‑TCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAAc                                                 >  1:1395458/1‑150 (MQ=255)
                                                                                                         ctgCTGGTTGCAAAGTTGGAACAGAAGGAAGGGGAAAATGATTTCCCC‑TGGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTg                                              <  2:2166627/148‑1 (MQ=255)
                                                                                                         cgccTGGTTCCCCAGTTGGAACAGCAGGAAGGGAAAAATTTTTTCCCC‑CCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTg                                              <  2:3751189/147‑1 (MQ=255)
                                                                                                         cAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCC‑TCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTg                                              >  1:1501890/1‑150 (MQ=255)
                                                                                                         cAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCC‑TCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTg                                              >  1:930534/1‑150 (MQ=255)
                                                                                                          aGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTCCCC‑TCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGc                                             <  1:2476982/150‑1 (MQ=255)
                                                                                                             tGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCC‑TCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTc                                          >  1:702097/1‑150 (MQ=255)
                                                                                                              ggTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCC‑TCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTct                                         >  2:4263182/1‑150 (MQ=255)
                                                                                                                  gCACAGATGGAACAGCAGGAAGGGGAAAATGATTTCCCC‑TCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTctggcc                                     <  2:1613153/150‑1 (MQ=255)
                                                                                                                  gCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCC‑TCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTctggcc                                     >  1:4020903/1‑150 (MQ=255)
                                                                                                                    agaaaaggACCAGCAGGAA‑GGGAAAATGATTTCCCCTTCGTCCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTctggcctg                                   <  1:1039025/144‑1 (MQ=255)
                                                                                                                    acaGATGGAACAGCAGGAAGGGGAAAATGATTTACCC‑TCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTctggcctg                                   >  1:3604503/1‑150 (MQ=255)
                                                                                                                    acaGAGGGAACAGAAGGAAGGGAAAAATGATTCCCCC‑TCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTctggcctg                                   <  2:72941/150‑1 (MQ=255)
                                                                                                                     caGATGGAACAGCAGGAAGGGGAAAATGATTTACCC‑TCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTctggcctgg                                  >  1:4118827/1‑150 (MQ=255)
                                                                                                                     caGATGGAACAGCAGGAAGGGAAAAATGTTTTCCCC‑TCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTctggcctgg                                  <  2:3515236/150‑1 (MQ=255)
                                                                                                                      aGATGGAACAGCAGGAAGGGGAAAATGATTTACCC‑TCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTctggcctggc                                 >  2:1525326/1‑150 (MQ=255)
                                                                                                                       gATGAAACAGAAGGAAGGGAAAAATGATTTCCCC‑TCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCctggct                                <  2:575700/150‑1 (MQ=255)
                                                                                                                        aTGGAACAGCAGGAAGGGGAAAATGATTTACCC‑TCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCctggctg                               >  2:2534527/1‑150 (MQ=255)
                                                                                                                         tGGAACAGCGGGAGGGGAAAAATGATTTCCCC‑GCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCctggctgg                              <  2:909700/150‑1 (MQ=255)
                                                                                                                            aaCAGCAGGAGGGGGAAAATGATTTCCCC‑TCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTggagg                           <  1:2356439/150‑1 (MQ=255)
                                                                                                                            aaCAGCAGGAAGGGGAAAATGATTTACCC‑TCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTggagg                           <  1:2523268/150‑1 (MQ=255)
                                                                                                                            aaCAGCAGGAAGGGGAAAATGATTTACCC‑TCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTggagg                           >  2:861669/1‑150 (MQ=255)
                                                                                                                            aaCAGCAGGAAGGGGAAAATGATTTACCC‑TCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTggagg                           >  1:1331045/1‑150 (MQ=255)
                                                                                                                                 cagGAAGGGGAAAATGATTTACCC‑TCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCAt                      >  2:823345/1‑150 (MQ=255)
                                                                                                                                   ggAAGGGGAAAATGATTTACCC‑TCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCAttt                    >  2:2038135/1‑150 (MQ=255)
                                                                                                                                     aaGGGGAAAATGATTTACCC‑TCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTg                  >  1:1353047/1‑150 (MQ=255)
                                                                                                                                      aGGGGAAAATGATTTACCC‑TCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGc                 >  1:3045750/1‑150 (MQ=255)
                                                                                                                                      aGGGGAAAATGATTTACCC‑TCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGc                 >  1:507701/1‑150 (MQ=255)
                                                                                                                                        gggAAAATGATTTACCC‑TCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCg               >  2:1537609/1‑150 (MQ=255)
                                                                                                                                        gaaaaatTGATTTCCCC‑TCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCg               <  1:2053022/147‑1 (MQ=255)
                                                                                                                                         ggAAAATGATTTACCC‑TCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGa              >  1:3182552/1‑150 (MQ=255)
                                                                                                                                           aaaaTGATTTACCC‑TCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATg            >  1:2586463/1‑150 (MQ=255)
                                                                                                                                           aaaaTGATTTACCC‑TCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATg            >  2:4102076/1‑150 (MQ=255)
                                                                                                                                               tGATTTACCC‑TCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACTTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAGGt        >  1:2645213/1‑150 (MQ=255)
                                                                                                                                                gATTTACCC‑TCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAGGTg       >  1:1764044/1‑150 (MQ=255)
                                                                                                                                                  tttACCC‑TCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAGGTGaa     >  1:1930788/1‑150 (MQ=255)
                                                                                                                                                  tttACCC‑TCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAGGTGaa     >  2:1293462/1‑150 (MQ=255)
                                                                                                                                                    tACCC‑TCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAGGTGAAtt   <  1:3086188/150‑1 (MQ=255)
                                                                                                                                                     aCCC‑TCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAGGTGAATTg  >  2:4136356/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
CGATTTCAGTTTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCC‑TCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAGGTGAATTG  >  AP012306/3527219‑3527517

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑