Predicted mutation
evidence seq id position mutation annotation gene description
RA AP012306 1,881,981 C→T intergenic (‑80/+32) yojL ← / ← ompC predicted thiamine biosynthesis lipoprotein/outer membrane porin protein C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123061,881,9810CT100.0% 1108.6 / NA 278intergenic (‑80/+32)yojL/ompCpredicted thiamine biosynthesis lipoprotein/outer membrane porin protein C
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (144/134);  total (144/134)

Only 100 of 283 total aligned reads displayed.

TTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATG  >  AP012306/1881834‑1882127
                                                                                                                                                   |                                                                                                                                                  
ttttGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGtcc                                                                                                                                                  <  2:2160892/150‑1 (MQ=255)
  ttGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGTCCGc                                                                                                                                                >  2:2543334/1‑150 (MQ=255)
   tGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGTCCGCa                                                                                                                                               >  1:2073367/1‑150 (MQ=255)
            ccaacAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGTCCGCAGGCCCTTTg                                                                                                                                      <  1:1856416/150‑1 (MQ=255)
                   agaagaGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGTCCGCAGGCCCTTTGTTCGata                                                                                                                               >  1:4106104/1‑150 (MQ=255)
                   agaagaGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGTCCGCAGGCCCTTTGTTCGata                                                                                                                               >  2:642298/1‑150 (MQ=255)
                   agaagaGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGTCCGCAGGCCCTTTGTTCGata                                                                                                                               <  1:729425/150‑1 (MQ=255)
                   agaagaGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGTCCGCAGGCCCATTGTTCGata                                                                                                                               >  1:4139789/1‑150 (MQ=255)
                    gaagaGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGTCCGCAGGCCCTTTGTTCGatat                                                                                                                              <  2:3914870/150‑1 (MQ=255)
                        agCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGTCCGCAGGCCCTTTGTTCGATATCAAt                                                                                                                          >  1:2374644/1‑150 (MQ=255)
                        agCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGTCCGCAGGCCCTTTGTTCGATATCAAt                                                                                                                          <  1:2352718/150‑1 (MQ=255)
                           gcgGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGTCCGCAGGCCCTTTGTTCGATATCAATCga                                                                                                                       <  2:40232/150‑1 (MQ=255)
                           gcgGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGTCCGCAGGCCCTTTGTTCGATATCAATCga                                                                                                                       <  2:3089705/150‑1 (MQ=255)
                             ggCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGTCCGCAGGCCCTTTGTTCGATATCAATCgaga                                                                                                                     <  1:1085619/150‑1 (MQ=255)
                             ggCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGTCCGCAGGCCCTTTGTTCGATATCAATCgaga                                                                                                                     >  2:26537/1‑150 (MQ=255)
                                agccagcAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGTCCGCAGGCCCTTTGTTCGATATCAATCGAGATTa                                                                                                                  <  1:2611902/150‑1 (MQ=255)
                                agccagcAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGTCCGCAGGCCCTTTGTTCGATATCAATCGAGATTa                                                                                                                  >  2:686045/1‑150 (MQ=255)
                                   cagcagTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGTCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGaa                                                                                                               <  2:1612486/150‑1 (MQ=255)
                                   cagcagTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGTCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGaa                                                                                                               >  1:3371009/1‑150 (MQ=255)
                                    agcagTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGTCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAAc                                                                                                              >  2:2441306/1‑150 (MQ=255)
                                      cagTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGTCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTg                                                                                                            <  1:2452798/150‑1 (MQ=255)
                                      cagTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGTCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTg                                                                                                            >  1:2518210/1‑150 (MQ=255)
                                      cagTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGTCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTg                                                                                                            <  1:1438471/150‑1 (MQ=255)
                                       agTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGTCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTgg                                                                                                           >  1:3730225/1‑150 (MQ=255)
                                           ccACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGTCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTaaa                                                                                                       <  2:2797274/150‑1 (MQ=255)
                                                cgGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGTCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGa                                                                                                  >  1:3315761/1‑150 (MQ=255)
                                                cgGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGTCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGa                                                                                                  <  1:1178684/150‑1 (MQ=255)
                                                cgGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGTCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGa                                                                                                  >  1:3073653/1‑150 (MQ=255)
                                                cgGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGTCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGa                                                                                                  >  1:2900719/1‑150 (MQ=255)
                                                cgGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGTCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGa                                                                                                  <  2:2633649/150‑1 (MQ=255)
                                                cgGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACCGTCGCAAGAGTACCCCAAAAAACTTACTTTCTACGCTGAAACCAATGAAAAAAGGGTCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGa                                                                                                  <  2:1230581/150‑1 (MQ=255)
                                                 gggTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGTCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGAc                                                                                                 <  2:125525/150‑1 (MQ=255)
                                                  ggTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGTCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGAcc                                                                                                >  2:1309666/1‑150 (MQ=255)
                                                    tAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGTCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCa                                                                                              >  2:1373387/1‑150 (MQ=255)
                                                     aaaGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGTCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCag                                                                                             >  1:480660/1‑150 (MQ=255)
                                                     aaaGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGTCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCag                                                                                             >  2:3141593/1‑150 (MQ=255)
                                                      aaGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGTCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCaga                                                                                            >  1:4165040/1‑150 (MQ=255)
                                                       aGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGTCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCagag                                                                                           <  1:3575829/150‑1 (MQ=255)
                                                       aGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGTCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCagag                                                                                           <  1:965628/150‑1 (MQ=255)
                                                         cTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGTCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCt                                                                                         >  2:2705363/1‑150 (MQ=255)
                                                            aTTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGTCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACg                                                                                      >  1:2741141/1‑150 (MQ=255)
                                                                 cATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGTCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGtt                                                                                 <  2:4070750/150‑1 (MQ=255)
                                                                  aTACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGTCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTa                                                                                <  2:1971624/150‑1 (MQ=255)
                                                                   tACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGTCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTAt                                                                               >  2:439631/1‑150 (MQ=255)
                                                                   tACGTGATTATCCTCATGCGAACGGCCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGTCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTAt                                                                               >  2:911965/1‑150 (MQ=255)
                                                                    aCGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGTCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATc                                                                              >  1:1619595/1‑150 (MQ=255)
                                                                    aCGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGTCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATc                                                                              >  2:3206069/1‑150 (MQ=255)
                                                                        gATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGTCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAgtg                                                                          <  1:2206593/150‑1 (MQ=255)
                                                                           tATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGTCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTg                                                                       >  2:2853121/1‑150 (MQ=255)
                                                                             tCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGTCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGAt                                                                     <  1:1501894/150‑1 (MQ=255)
                                                                              ccTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGTCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATg                                                                    <  2:597041/150‑1 (MQ=255)
                                                                               cTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGTCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGc                                                                   >  2:1816698/1‑150 (MQ=255)
                                                                                tCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGTCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGcc                                                                  <  1:3835606/150‑1 (MQ=255)
                                                                                 cATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGTCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCa                                                                 >  2:3406951/1‑150 (MQ=255)
                                                                                      gAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGTCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTc                                                            >  1:1543563/1‑150 (MQ=255)
                                                                                            tCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGTCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGt                                                      >  1:3790651/1‑150 (MQ=255)
                                                                                             cGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGTCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTg                                                     >  1:864145/1‑150 (MQ=255)
                                                                                             cGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGTCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTg                                                     >  2:3980883/1‑150 (MQ=255)
                                                                                               cAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGTCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTTaa                                                   >  1:2936646/1‑150 (MQ=255)
                                                                                               cAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGTCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGaa                                                   >  2:3510015/1‑150 (MQ=255)
                                                                                                 agagTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGTCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACt                                                 >  2:3281745/1‑150 (MQ=255)
                                                                                                    gTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGTCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGt                                              <  2:1089795/150‑1 (MQ=255)
                                                                                                     tACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGTCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGtt                                             >  1:1198723/1‑150 (MQ=255)
                                                                                                         ccAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGTCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTgtcg                                         <  1:3934529/150‑1 (MQ=255)
                                                                                                          cAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGTCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTgtcgt                                        <  1:4244399/150‑1 (MQ=255)
                                                                                                           aaaaaaCTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGTCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTgtcgtc                                       <  1:3548572/150‑1 (MQ=255)
                                                                                                             aaaaCTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGTCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCca                                     <  1:1113835/150‑1 (MQ=255)
                                                                                                              aaaCTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGTCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCcag                                    <  2:2338388/150‑1 (MQ=255)
                                                                                                              aaaCTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGTCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCcag                                    <  2:2491955/150‑1 (MQ=255)
                                                                                                              aaaCTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGTCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCcag                                    >  1:816558/1‑150 (MQ=255)
                                                                                                               aaCTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGTCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCcagc                                   <  2:1760785/150‑1 (MQ=255)
                                                                                                                 cTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGTCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCcagcag                                 >  2:2752776/1‑150 (MQ=255)
                                                                                                                  tGAGTTTGTACGCTGAAAACAATGAAAAAAGGGTCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAgg                                <  1:3486146/150‑1 (MQ=255)
                                                                                                                   gAGTTTGTACGCTGAAAACAATGAAAAAAGGGTCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGt                               >  1:3260754/1‑150 (MQ=255)
                                                                                                                   gAGTTTGTACGCTGAAAACAATGAAAAAAGGGTCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGt                               >  2:859186/1‑150 (MQ=255)
                                                                                                                   gAGTTTGTACGCTGAAAACAATGAAAAAAGGGTCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGt                               >  2:792950/1‑150 (MQ=255)
                                                                                                                    aGTTTGTACGCTGAAAACAATGAAAAAAGGGTCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGtt                              >  2:1529796/1‑150 (MQ=255)
                                                                                                                    aGTTTGTACGCTGAAAACAATGAAAAAAGGGTCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGtt                              >  2:4253539/1‑150 (MQ=255)
                                                                                                                     gTTTGTACGCTGAAAACAATGAAAAAAGGGTCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTg                             >  1:1352032/1‑150 (MQ=255)
                                                                                                                      tttGTACGCTGAAAACAATGAAAAAAGGGTCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGa                            <  1:1282147/150‑1 (MQ=255)
                                                                                                                       ttGTACGCTGAAAACAATGAAAAAAGGGTCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGAt                           <  2:4096080/150‑1 (MQ=255)
                                                                                                                        tGTACGCTGAAAACAATGAAAAAAGGGTCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGAtt                          >  2:1909661/1‑150 (MQ=255)
                                                                                                                        tGTACGCTGAAAACAATGAAAAAAGGGTCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGAtt                          <  1:2805455/150‑1 (MQ=255)
                                                                                                                          tACGCTGAAAACAATGAAAAAAGGGTCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGAtttt                        <  2:48555/150‑1 (MQ=255)
                                                                                                                           aCGCTGAAAACAATGAAAAAAGGGTCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTg                       >  1:1776212/1‑150 (MQ=255)
                                                                                                                           aCGCTGAAAACAATGAAAAAAGGGTCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTg                       <  1:2277959/150‑1 (MQ=255)
                                                                                                                             gCTGAAAACAATGAAAAAAGGGTCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGTGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTa                     <  2:528066/150‑1 (MQ=255)
                                                                                                                                 aaaaCAATGAAAAAAGGGTCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAAATGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCa                 <  1:1049142/150‑1 (MQ=255)
                                                                                                                                  aaaCAATGAAAAAAGGGTCCGCAGGCCCTTTGTTCGGTATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCaa                <  1:3618778/150‑1 (MQ=255)
                                                                                                                                  aaaCAATGAAAAAAGGGTCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAATGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCaa                >  2:3819953/1‑150 (MQ=255)
                                                                                                                                   aaCAATGAAAAAAGGGTCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAAc               <  2:3658340/150‑1 (MQ=255)
                                                                                                                                   aaCAATGAAAAAAGGGTCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAAc               <  1:1704569/150‑1 (MQ=255)
                                                                                                                                      aaTGAAAAAAGGGTCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTa            >  2:2034733/1‑150 (MQ=255)
                                                                                                                                      aaTGAAAAAAGGGTCCGCAGGCACTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTa            <  1:3174333/150‑1 (MQ=255)
                                                                                                                                         gAAAAAAGGGTCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGt         <  2:1182238/150‑1 (MQ=255)
                                                                                                                                          aaaaaaGGGTCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTg        >  1:4022507/1‑150 (MQ=255)
                                                                                                                                              aaGGGTCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACa    >  2:4225601/1‑150 (MQ=255)
                                                                                                                                               aGGGTCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACAt   >  2:4154641/1‑150 (MQ=255)
                                                                                                                                               aGGGTCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACAt   >  2:1432151/1‑150 (MQ=255)
                                                                                                                                                gggTCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATg  >  1:2531889/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATG  >  AP012306/1881834‑1882127

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑