Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123061,944,2880AC36.1% 377.5 / 50.7 206G361G (GGT→GGG) menEo‑succinylbenzoate‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base A (83/48);  new base C (0/74);  total (83/123)
Fisher's exact test for biased strand distribution p-value = 3.70e-23
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 270 total aligned reads displayed.

ACCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAA‑CACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCAGTTGCCCGTTACGCCAGTAACCTTCTGC  >  AP012306/1944139‑1944434
                                                                                                                                                      |                                                                                                                                                  
    aTTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAA‑CACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCct                                                                                                                                                <  1:2331710/150‑1 (MQ=255)
      tCACTAAGATCAACGCTCTCGTGGTCATGCTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAA‑CACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCctct                                                                                                                                              <  1:1597185/150‑1 (MQ=255)
      tCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAA‑CACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCctct                                                                                                                                              >  2:4074049/1‑150 (MQ=255)
       cACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAA‑CACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCtctc                                                                                                                                             >  1:4094932/1‑150 (MQ=255)
        tcTAAGATCAACTTTCTCGTGGTTTTACTCCATCCCCGCCACCGTTAGATGACCAAACTCCTTCTCGGCAACGGGGACGATAAA‑CGCCTGCAAAGCCGCAGGATGTGCAGCAATTACGCGCTCGACTTAATCCGGCTGAATTCCCTCTcc                                                                                                                                            <  2:3310578/149‑1 (MQ=255)
        aCTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAA‑CACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTcc                                                                                                                                            <  1:3555534/150‑1 (MQ=255)
         cTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAA‑CACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCg                                                                                                                                           <  1:1006222/150‑1 (MQ=255)
         cTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAA‑CACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCg                                                                                                                                           <  1:3303243/150‑1 (MQ=255)
          tAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAA‑CACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGc                                                                                                                                          >  1:3651987/1‑150 (MQ=255)
           aaGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAA‑CACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGcc                                                                                                                                         <  2:2800971/150‑1 (MQ=255)
             gATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAA‑CACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCAc                                                                                                                                       >  1:3398206/1‑150 (MQ=255)
              aTCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAA‑CACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACt                                                                                                                                      >  2:2153557/1‑150 (MQ=255)
              aTCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAA‑CACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACt                                                                                                                                      >  1:1423177/1‑150 (MQ=255)
               tCACCGCTCTCGTGGTCATACCCCACCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAA‑CCCCTGCAAACCCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATCCCCTCTCCGCCACTg                                                                                                                                     <  2:486998/150‑1 (MQ=255)
               tCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAA‑CACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTg                                                                                                                                     >  2:3402123/1‑150 (MQ=255)
                 aaCGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAA‑CACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTgaa                                                                                                                                   >  1:2882904/1‑150 (MQ=255)
                 aaCGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAA‑CACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTgaa                                                                                                                                   >  2:585919/1‑150 (MQ=255)
                  aCGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAA‑CACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTCCCGGCTGAATACCCTCTCCGCCACTgaag                                                                                                                                  <  1:2666493/150‑1 (MQ=255)
                    gCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAA‑CACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTgaagaa                                                                                                                                <  1:3211613/150‑1 (MQ=255)
                       ctcGTGGTCATCCTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAA‑CACCTGCAAACCCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAg                                                                                                                             <  1:1854811/150‑1 (MQ=255)
                       ctcGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAA‑CACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAg                                                                                                                             >  1:551373/1‑150 (MQ=255)
                        tcGTGGTCATCCTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAA‑CACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGa                                                                                                                            <  2:2735936/150‑1 (MQ=255)
                        tcGTGGTCATACCCCATCCCCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAC‑CCCCTGCAAACCCGCAGGAGGTCCAGCAATTACGCGCTCGCCTTCTCCCGGCTGAATCCCTTCTCCGCCACTGAAGAATAGa                                                                                                                            <  2:1506192/150‑1 (MQ=255)
                         cGTGGTCATACCCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAA‑CCCCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGAt                                                                                                                           <  2:2964253/150‑1 (MQ=255)
                            ggTCATACTCCACCCCCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAA‑CCCCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGt                                                                                                                        <  2:1402267/150‑1 (MQ=255)
                              tCATACTCCATCCCCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAA‑CACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCt                                                                                                                      <  1:3532228/150‑1 (MQ=255)
                              tCAGACCCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAA‑CACCTGCAAAACCGCAGGAGGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCt                                                                                                                      <  2:2896019/150‑1 (MQ=255)
                                atcccccATCCCCCCCCCCGGTCGATGACCAACCTCCTTCTCGCCACCGGCCACGATAAC‑CCCCTCCAAACCCCCAGGATCTCCACCAATTACGCCCTCCACTTCTTCCGCCTAAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTaa                                                                                                                    <  2:3158468/145‑1 (MQ=255)
                                 tccTCCACCCCCGCCACCGGTGGATGACCAAACTCTTTGTCGGCAACGGGAACAATAAC‑CCCCTGCAAAACCGCAGGATGTACAGCAATTACGCGCTCATTTTCTTCGGGCTGAATCCCCTCCCCGCCACTGAAGAATAGATTGTCTaaa                                                                                                                   <  2:2193925/148‑1 (MQ=255)
                                  aCTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAA‑CACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAAc                                                                                                                  >  1:3724700/1‑150 (MQ=255)
                                    tCCATCCCCGCCACCGGTCGATGACCAACCTCCTTGTCGGCAACGGGGACGATAAA‑CCCCTGCAAACCCGCAGGATGTGCAGCAATTCCGCGCTCGACTTCTCCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGt                                                                                                                <  1:1360311/150‑1 (MQ=255)
                                     ccATCCCCGCCACCGGTCGATGACCAACCTCCTTGTCGGCAACGGGGACGATAAA‑CACCTGCAAACCCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTc                                                                                                               <  1:491530/150‑1 (MQ=255)
                                      cATCCCCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAA‑CACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCATCTTCTTCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTcc                                                                                                              <  2:2686209/150‑1 (MQ=255)
                                         ccccGCCCCCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAC‑CCCCTGCAAACCCGCAGGATGTGCAGAAATTACGCGCTCGACTTCTCCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGAc                                                                                                           <  2:3470188/148‑1 (MQ=255)
                                          aCCGCCACCGGTCGATGACCAACCTCCTTGTCGGCAACGGGGACGATAAA‑CACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTCCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACa                                                                                                          <  1:1613338/150‑1 (MQ=255)
                                            cGCCCCGGGTGGATGACCAACCCCCTTGTCGGAAAGGGGGACGATAAA‑CACCTGCAAAACCGCAGGATGTGCAGAATTTACGCGCTCGACTTCTCCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAAt                                                                                                        <  2:2018806/150‑1 (MQ=255)
                                              ccACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAA‑CCCCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATgg                                                                                                      <  2:2505421/150‑1 (MQ=255)
                                              ccACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAA‑CACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATgg                                                                                                      <  1:1317532/150‑1 (MQ=255)
                                               cACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAA‑CACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGt                                                                                                     >  1:1986965/1‑150 (MQ=255)
                                                aCCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAA‑CACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTc                                                                                                    >  2:1473908/1‑150 (MQ=255)
                                                aCCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAA‑CACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTc                                                                                                    >  1:3355329/1‑150 (MQ=255)
                                                  cGGTCAATGCCCAAACTCCTTTTCGACAACGGGGACGATAAA‑CACCTAAAAAACCGCAGGATGTACAGCAATTACGCGCTCGACTTCTCCCGCCTAAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAg                                                                                                  <  2:761921/150‑1 (MQ=255)
                                                  cGGCCGATGACCAACCTCCTTGTCGGCAACGGGGACGATAAA‑CCCCTGCAAAACCGCAGGATGGGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAg                                                                                                  <  1:651259/150‑1 (MQ=255)
                                                      cGATGCCCAAACTCCTTGTCGGCAACGGGGACGATAAA‑CCCCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTCCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTg                                                                                              <  2:3280036/150‑1 (MQ=255)
                                                         tGACCAAACTCCTTGTCGGCAAGGGGGACGATAAA‑CCCCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGCTTTCTTCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCa                                                                                           <  2:2231811/150‑1 (MQ=255)
                                                          gACCAACCTCCTTGTCGGCAACGGGGACGATAAC‑CCCCTGCAAACCCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCAt                                                                                          <  2:87434/150‑1 (MQ=255)
                                                            ccAAACTCCTTGTCGGCAACGGGGACGATAAA‑CACCTGCAAACCCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTa                                                                                        <  2:2710553/150‑1 (MQ=255)
                                                             cAAACTCCTTGTCGGCAACGGGGACGATAAA‑CACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTAt                                                                                       <  1:4026410/150‑1 (MQ=255)
                                                              aaaCTCCTTGTCGGCAACGGGGACGATAAA‑CCCCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATg                                                                                      <  1:1495694/150‑1 (MQ=255)
                                                              aaaCTCCTTGTCGGCAACGGGGACGATAAA‑CACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATg                                                                                      <  1:862314/150‑1 (MQ=255)
                                                                 ctttttttCGGGAAGGGGGAC‑ATAAACCGCCGGCAAAACCGCAGGATGTTAAGATATTACGCGCTGCACTCCTTCCGACTACTAACCCCCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCAt                                                                                   <  2:1264122/143‑1 (MQ=255)
                                                                  tCCTTGTCGCCAACGGGTACGATAAC‑CACCTGCAAACCCGCAGGATGTGCAGCAATTCCCGGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCAtc                                                                                  <  2:322208/150‑1 (MQ=255)
                                                                   ccTTGTCGGCAACGGGGACGATAAA‑CACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCAtct                                                                                 <  1:3011178/150‑1 (MQ=255)
                                                                    cTTGTCGGCAAGGGGGCCGATAAA‑CCCCTGCAAACCCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTCCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCAtctc                                                                                <  2:948139/150‑1 (MQ=255)
                                                                    cTTGTCGGCAACGGGGACGATAAA‑CACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCAtctc                                                                                >  2:3074080/1‑150 (MQ=255)
                                                                      tgccGGCAACGGGAACGATAAA‑CACCTGCAAACCCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCAc                                                                              <  1:3684688/147‑1 (MQ=255)
                                                                       gTCGGCAACGGGGACGATAAA‑CACCTGCAAACCCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCAcc                                                                             <  1:521486/150‑1 (MQ=255)
                                                                       gTCGGCAACGGGGACGAAAAA‑CACCTGAAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCAcc                                                                             <  2:3274447/150‑1 (MQ=255)
                                                                            cAAGGGGGACGATAAA‑CACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGTCTTCTTCCGGCTAAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGAt                                                                        <  2:2347906/150‑1 (MQ=255)
                                                                             aaCGGGGACGATAAA‑CACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTCCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATc                                                                       <  2:1797303/150‑1 (MQ=255)
                                                                                  ggACGATAAA‑CACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATcgcgcg                                                                  >  1:2108194/1‑150 (MQ=255)
                                                                                   gACGATAAA‑CACCTGCAAAACCGCAGGATGTGCAGCAATTCCGCGCTCGACTTCTCCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCgcgt                                                                 <  2:2655656/150‑1 (MQ=255)
                                                                                      gaaaaa‑CCCCTGCAAAACCGCAGGTTGGCCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATCCCCTCCCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCgcgtagc                                                              <  2:1873204/147‑1 (MQ=255)
                                                                                      gATAAA‑CACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCgcgtagc                                                              <  1:285600/150‑1 (MQ=255)
                                                                                       aTAAA‑CACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCgcgtagcg                                                             <  1:1218679/150‑1 (MQ=255)
                                                                                        tAAA‑CACCTGCAAACCCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCgcgtagcgt                                                            <  1:2986805/150‑1 (MQ=255)
                                                                                             ccccTGAAAAACCGCAGGATGGGCAGCAATTACGCGCTCGACTTCTTCCGGCGGAATTCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCa                                                        <  2:370206/148‑1 (MQ=255)
                                                                                                   cAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTc                                                  >  2:2544399/1‑150 (MQ=255)
                                                                                                    aaaaCCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCg                                                 <  1:2459905/150‑1 (MQ=255)
                                                                                                        ccGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCAt                                             >  1:1264473/1‑150 (MQ=255)
                                                                                                        ccGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCAt                                             <  1:3328487/150‑1 (MQ=255)
                                                                                                           cAGGATGTCCAGCAATTACCCCCCCCTCTTCTCCCCGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTaa                                          <  2:3657990/150‑1 (MQ=255)
                                                                                                           cAGCATGTGCAACAATTATGGCCCGGCCTTCGGCGGGCGCAATCCCCACTCCGCCACTGAAGAATAGGTTGGCTAAACGTCCGAGAATGGTCAGCTTGCCATTATGCATCTGACCGCGATCGCGCGTAGCGTAGCAGCCTTCGTCATTaa                                          <  2:1570248/150‑1 (MQ=255)
                                                                                                            gggATGTGCAGCAATTACGCGCTCGATTTCTTCCGGCTAAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAAc                                         <  2:1961449/149‑1 (MQ=255)
                                                                                                            aGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAAc                                         >  2:373406/1‑150 (MQ=255)
                                                                                                             ggATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAAcc                                        >  2:1828785/1‑150 (MQ=255)
                                                                                                                  tgCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGa                                   <  1:224738/150‑1 (MQ=255)
                                                                                                                  tgCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGa                                   >  1:3936899/1‑150 (MQ=255)
                                                                                                                    cagcaATTACGCGCTCGACTTTTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGaaa                                 >  1:9252/1‑150 (MQ=255)
                                                                                                                    cagcaATTACGCGCTCGACTTCTTCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGaaa                                 <  2:1033388/150‑1 (MQ=255)
                                                                                                                     agcaATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAAc                                >  2:1917920/1‑150 (MQ=255)
                                                                                                                      gcaATTACGCGCTCGACTTCTTCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAAcc                               <  1:799962/150‑1 (MQ=255)
                                                                                                                       caATTACGCGCTCGACTTCTCCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCa                              <  2:3522905/150‑1 (MQ=255)
                                                                                                                          ttACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCAGtt                           >  1:1008620/1‑150 (MQ=255)
                                                                                                                          ttACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCAGtt                           <  1:1100797/150‑1 (MQ=255)
                                                                                                                           tACGCGCTCGACTTCTTCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCAGTTg                          <  1:425097/150‑1 (MQ=255)
                                                                                                                            ccgcgCTCGACTTCTCCCGGCTGAATACCCCCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCAGTTGc                         <  2:1174494/149‑1 (MQ=255)
                                                                                                                            aCGCGCTCGACTTCTTCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCAGTTGc                         <  1:1718669/150‑1 (MQ=255)
                                                                                                                             cggccTCGGCTTCTCCCGGTCGAACCCCCACCCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCAGTTGcc                        <  2:3440162/146‑1 (MQ=255)
                                                                                                                               cgcTCGACTTCTTCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCAGTTGCCCg                      <  1:3068456/150‑1 (MQ=255)
                                                                                                                                  tCGATTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCAGTTGCCCGTTa                   <  1:1332534/150‑1 (MQ=255)
                                                                                                                                      cttcttCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCAGTTGCCCGTTACGcc               <  1:2231605/150‑1 (MQ=255)
                                                                                                                                         cttcCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCAGTTGCCCGTTACGCCAGt            <  2:1058099/150‑1 (MQ=255)
                                                                                                                                         ctgccGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCAGTTGCCCGTTACGCCAGt            <  1:2209720/147‑1 (MQ=255)
                                                                                                                                          ttcCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCAGTTGCCCGTTACGCCAGTa           <  2:430404/150‑1 (MQ=255)
                                                                                                                                              ggCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCAGTTGCCCGTTACGCCAGTAACCt       >  2:2182448/1‑150 (MQ=255)
                                                                                                                                               gCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCAGTTGCCCGTTACGCCAGTAACCtt      <  1:4058172/150‑1 (MQ=255)
                                                                                                                                               gCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCAGTTGCCCGTTACGCCAGTAACCtt      >  1:238261/1‑150 (MQ=255)
                                                                                                                                                  gAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCAGTTGCCCGTTACGCCAGTAACCTTCTg   >  2:3796355/1‑150 (MQ=255)
                                                                                                                                                   aaTACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCAGTTGCCCGTTACGCCAGTAACCTTCTGc  >  1:3762735/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                  
ACCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAA‑CACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCAGTTGCCCGTTACGCCAGTAACCTTCTGC  >  AP012306/1944139‑1944434

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑