Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123063,903,7910AG25.7% 568.4 / 70.4 245E189G (GAA→GGA) yjgJpredicted transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (19/163);  new base G (63/0);  total (82/163)
Fisher's exact test for biased strand distribution p-value = 7.50e-42
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 352 total aligned reads displayed.

GACCATTTATGACTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAACGTT  >  AP012306/3903642‑3903940
                                                                                                                                                     |                                                                                                                                                     
  ccATTTATGACTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAg                                                                                                                                                     >  2:2443540/1‑150 (MQ=255)
      ttATGACTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCggt                                                                                                                                                 >  2:940269/1‑148 (MQ=255)
       tATGACTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATa                                                                                                                                                >  1:648986/1‑150 (MQ=255)
        aTGACTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATaa                                                                                                                                               <  2:1205001/150‑1 (MQ=255)
          gACTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATaaaa                                                                                                                                             >  1:2254560/1‑150 (MQ=255)
          gACTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATaaaa                                                                                                                                             <  1:1965158/150‑1 (MQ=255)
            cTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATaaaaaa                                                                                                                                           >  1:3401083/1‑149 (MQ=255)
            cTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGAGATaaaaaa                                                                                                                                           >  2:1611109/1‑149 (MQ=255)
            cTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAAc                                                                                                                                           <  1:716965/150‑1 (MQ=255)
             tACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATaaaaaat                                                                                                                                          >  2:677413/1‑148 (MQ=255)
             tACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACt                                                                                                                                          <  1:3972916/150‑1 (MQ=255)
              aCATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTa                                                                                                                                         <  1:3478969/150‑1 (MQ=255)
               cATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGCAGGGATAAAAAatat                                                                                                                                        >  1:3516502/1‑150 (MQ=255)
                aTCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAACTAtt                                                                                                                                       >  1:2770597/1‑150 (MQ=255)
                aTCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAACGGTAAAAAATaat                                                                                                                                       >  2:1937883/1‑148 (MQ=255)
                aTCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTAtt                                                                                                                                       <  1:2866185/150‑1 (MQ=255)
                aTCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTAtt                                                                                                                                       <  1:1189992/150‑1 (MQ=255)
                 tCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTc                                                                                                                                      <  2:3396969/150‑1 (MQ=255)
                   gCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCtt                                                                                                                                    <  2:2698936/150‑1 (MQ=255)
                    ccAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTg                                                                                                                                   <  2:2275508/150‑1 (MQ=255)
                     cAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAACTATTCTTga                                                                                                                                  >  1:1628793/1‑150 (MQ=255)
                      aGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTgag                                                                                                                                 <  1:1052308/150‑1 (MQ=255)
                      aGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTgag                                                                                                                                 <  1:1518346/150‑1 (MQ=255)
                         cggcATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAACGCTAAAAAATATTCTTtaggag                                                                                                                              >  2:3061459/1‑150 (MQ=255)
                          ggcATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGAGATAAAAAATATTCTTGGGGAGt                                                                                                                             >  2:3312971/1‑150 (MQ=255)
                            cATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGa                                                                                                                           <  1:1382363/150‑1 (MQ=255)
                            cATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAAATATTCTTTAGGAGTGa                                                                                                                           >  1:3015442/1‑150 (MQ=255)
                                cacaACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGTGGGAGCGATAAAAAATATTCTTTAGGAGTGATGCg                                                                                                                       >  1:97894/1‑150 (MQ=255)
                                  caACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGGTAAAAACTATTCTTGAGGGGAGAGGCggg                                                                                                                     >  1:2523713/1‑150 (MQ=255)
                                     cgcgCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAAATATTATTAAGGAGTGCTGCGGGccc                                                                                                                  >  1:1469454/1‑149 (MQ=255)
                                      gcgcACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCtt                                                                                                                 <  2:11882/150‑1 (MQ=255)
                                      gcgcACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAAATATTTTTGAGGAGGGATGCGGGGCtt                                                                                                                 >  2:2486770/1‑150 (MQ=255)
                                        gcACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAAATATTCTTGAGGAGGGATGCGGGCCTTGa                                                                                                               >  2:2146976/1‑150 (MQ=255)
                                         cacaATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAAATATTCTTTAGGGGGGATGCGGGCCCTgtc                                                                                                              >  2:1996679/1‑148 (MQ=255)
                                            aaTGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAACGATAAAAAATATTTTTTAGGAGGGATGCGGGCCTTGGcccc                                                                                                           >  2:1811069/1‑149 (MQ=255)
                                               gtgtgACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAACTATTCTTTAGGAGTGATGCGGGCCTTGACCCGaaa                                                                                                        >  1:4040402/1‑150 (MQ=255)
                                               gtgtgACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGaaa                                                                                                        <  1:2431958/150‑1 (MQ=255)
                                                tgtgACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAAATATTCTTTAGGAGGGATGCGGGCCTTGACCCGaaaa                                                                                                       >  2:3541236/1‑149 (MQ=255)
                                                  tgACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGc                                                                                                     <  1:3182311/150‑1 (MQ=255)
                                                     cTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGGTAAAAAATATTTTTTAGGAGGGATGCGGGCCTTGACCCGAAAAGgggg                                                                                                  >  2:3704486/1‑150 (MQ=255)
                                                       gATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAAATATTCTTTAGGAGGGATGCGGGCCCTTACCCGAAAGGCgggga                                                                                                >  1:3088208/1‑148 (MQ=255)
                                                          tttATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAgc                                                                                             <  1:1096928/150‑1 (MQ=255)
                                                           ttATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGGGATAAAAACTATTTTTTTGGAGTGGTGGGGGCCTTTTCCCGAAAGGCGGGgaaaag                                                                                            >  2:1861145/1‑147 (MQ=255)
                                                           ttATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAAATATTCTTTAGGAGTGATGCGGGGCTTGACCCGAAAGGCGGGGAAggg                                                                                            >  2:1652615/1‑148 (MQ=255)
                                                             aTGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGGTAAAAACTATTCTTTTGGAGGGGTGGGGGGCTTGACCCGAAAGGCGGGgaaaagcc                                                                                          >  2:1389293/1‑145 (MQ=255)
                                                             aTGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGcc                                                                                          <  2:2828553/150‑1 (MQ=255)
                                                              tGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCt                                                                                         <  2:2653193/150‑1 (MQ=255)
                                                              tGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCt                                                                                         <  2:3934058/150‑1 (MQ=255)
                                                                aGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGc                                                                                       <  2:3816772/150‑1 (MQ=255)
                                                                 gTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGcc                                                                                      <  2:562594/150‑1 (MQ=255)
                                                                 gTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAAGCGGGAAAGCGCCTGcc                                                                                      >  1:1715806/1‑150 (MQ=255)
                                                                  tACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAAATATTCTTGAGGAGGGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCg                                                                                     >  1:416889/1‑150 (MQ=255)
                                                                   aCCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAAACATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAAGCGGGGAAGCGCCCGCCGa                                                                                    >  1:2630119/1‑150 (MQ=255)
                                                                       tGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAAATATTTTTGAGGAGGGATGCGGGGCTTGACCCGAAAAGCGGGGAAGCGCCCGCCGGTAAg                                                                                >  2:2629047/1‑150 (MQ=255)
                                                                           gTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGGGATAAAAAATATTTTTGAGGAGTGATGGGGGGCTTTACCCGAAAGGCGGGGAAGGGCCCGCCGGTaaagtgt                                                                            >  2:2383699/1‑145 (MQ=255)
                                                                           gTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAACACTTCTTGCGGGGTCAAGAAGGCCTTTCCCCGCAAGGGGGGGAAGCGCCCCCCGATaacatgt                                                                            >  2:1263790/1‑145 (MQ=255)
                                                                            tCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGGTAAAAAATATTCTTGAGGGGTGGTGCGGGGCTTTGGCCGAAAGGCGGGGAAACGCCCGCCGGTAAAAtgtg                                                                           >  2:4032555/1‑150 (MQ=255)
                                                                              tGGGCTTTCTGCGAAGGCAGGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCTTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGAt                                                                         <  2:1431386/150‑1 (MQ=255)
                                                                                  cTTTCTGCGAAGGCGCGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGa                                                                     <  1:2567759/150‑1 (MQ=255)
                                                                                    ttCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAAATATTCTTTTGGAGTGATGCGGGCCTTGACCCGAAAAGGGGGAAAAGGCCTGCCGATAAGTTTTGGTAAGACa                                                                   >  2:2599125/1‑150 (MQ=255)
                                                                                         cGAAGGCACGAGAGGGGCCCTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGGGGGCCTTGACCCAAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACaataat                                                              <  2:1330640/150‑1 (MQ=255)
                                                                                           aaGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCTTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTc                                                            <  2:866548/150‑1 (MQ=255)
                                                                                            aGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGGTAAAAAGTATTTTTTAGGAGGGATGCGGGCCCTGCCCCGAAAGGGGGGGAAAAGCCGCCCGGTAAATTGTGGTAAAAAAAAAATTCa                                                           >  2:887029/1‑150 (MQ=255)
                                                                                              gCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGCCCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCAcg                                                         <  1:920119/150‑1 (MQ=255)
                                                                                               cACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGGGGTTAAAAACATTCTTGGGGGGGGACGCGGGGCCTGGCCCGAAAAGCGGGGAAGCCACCGCCCATGAGTTGTGAGAACACAATAATTAAccc                                                        >  2:1486468/1‑148 (MQ=255)
                                                                                               cACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCAcgc                                                        <  1:1279823/150‑1 (MQ=255)
                                                                                                 cGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAACTATTCTTTAGGAGGGATGCGGGCCCTGACCCGAAAAGCGGGGAAACGCCCGCCCGTAAAGTGTGATAAAACAAAAAATTAcgcgg                                                      >  2:3122031/1‑149 (MQ=255)
                                                                                                  gagaGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAACGCCTGCCGATAAGTTGTGATAAGACAATAATTTACGCGtt                                                     >  1:2070838/1‑150 (MQ=255)
                                                                                                    gagGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAAATATTCTTGAGGAGGGATGCGGGCCTTGGCCCGAAAAGCGGGGAAAAGCCCGCCGGTAAAATGTGATAAAAAAAAAAATTACGCGttta                                                   >  2:2232484/1‑148 (MQ=255)
                                                                                                        ggCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCt                                               <  1:3915544/150‑1 (MQ=255)
                                                                                                         gCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGGGATAAAAAATATTTTTTAGGGGTGATGTGGGCCCTGACCCGAAAAGGGGGGAAACGCCTGGCGCTAAGATGTGGTAAGAAAATAAATCAACCGTTAAGGGTa                                              >  2:3514876/1‑150 (MQ=255)
                                                                                                         gCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCTTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTa                                              <  2:2957004/150‑1 (MQ=255)
                                                                                                          cACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAg                                             <  1:1744463/150‑1 (MQ=255)
                                                                                                            ctctATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCg                                           <  2:2089923/150‑1 (MQ=255)
                                                                                                              ctATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAAAAATTTTTGAGGGGGGATGCGGGCCCTGACCCCAAAGGCGGGGAAACGCCCGCCGGTAAGTTGTGGTAAAACAAAAAATCACGCGGTAAGGGTAGCggg                                         >  2:471610/1‑149 (MQ=255)
                                                                                                                 taGAACAACTCTGTGCAACAGCTGCAAGGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAAtt                                      <  1:894100/150‑1 (MQ=255)
                                                                                                                  aGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTg                                     <  1:3290375/150‑1 (MQ=255)
                                                                                                                    aacaacTCTGTGCAACAGCTGCAATGGCGGGGGGAACGATAAAAAAAATTTTTCTGGGGGGGTGCGGGCCTTTGACCGGAAGGCGGGGGAGCGCCCGCCCGCAAATTGTGGTAAGAAAAAAAATTACGCGTTAAGGGGAGAGGAAATGAt                                   >  2:714871/1‑150 (MQ=255)
                                                                                                                       aacTCGGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTAt                                <  1:181972/150‑1 (MQ=255)
                                                                                                                         ctctGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCt                              <  1:2323499/150‑1 (MQ=255)
                                                                                                                            tgtgCAACAGCTGCAATGGCGGGGGGAGCGATAAAAAAAATTCTTTAAGGGGGATGCGGGCCCTGACCCGGAAGGCGGGGAAACGCCCGCCCATAAGGTGTGATAAGACAAAAAATTACCCCTTAAGGGTAGCGGGAATGGTTATCtttt                           >  2:61365/1‑150 (MQ=255)
                                                                                                                             gtgCAACAGCTGCAATGGCGGGGGGAGCGATAAAAACTATTCTTTAGGAGTGATGCCGGGCTTGACCCGGAAGGCGGGGAAAGGCCCGCCGATAAGTTGTGATAAAACAAAAAATTAACCGGTAAAGGTAACGGGAATGATTAttttttt                          >  2:2850787/1‑149 (MQ=255)
                                                                                                                             gtgCAACAGCTGCAATGGCGGGGGGAGCGAAAAAAACTATTTTTTGGGGGGGGTGCGGGCCGTGGCCCCAAAAGGGGGAAAACGCCCGGCGGGAAGATGTGTTAAAACAAAAAATTACGCGTTAAAGGTAGCGGGATTGATTAttttttt                          >  2:3667317/1‑149 (MQ=255)
                                                                                                                                cAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGCCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGta                       <  2:323616/150‑1 (MQ=255)
                                                                                                                                   cAGCTGCAATGGCGGGGGGAGCGATAAAAAATATTTTTTAGGGGGGATGCGGGCCTTGACCCGAAAGGCGGGGAAACGCCCGCCGGTAAAGTGTGGTAAAACAAAAAATTACGCGTTTAGGCCAGCGGAAATGATTATCtttttttttaa                    >  2:1727473/1‑143 (MQ=255)
                                                                                                                                    aGCTGCAATGGCGGGGGAAGCAATAAAAACTATTCTTGGGGAGGGATAGGGACATTGACCCGAAGGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAAc                   <  2:412279/150‑1 (MQ=255)
                                                                                                                                     gCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACg                  <  2:3441884/150‑1 (MQ=255)
                                                                                                                                        gCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATa               <  1:2152062/150‑1 (MQ=255)
                                                                                                                                           agggCGGGGGAGGCAATAAAAGCTATTCTTGAGGAGTGATGCGGGCTTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGaa            <  2:3596749/148‑1 (MQ=255)
                                                                                                                                           aTGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGaa            <  2:4013664/150‑1 (MQ=255)
                                                                                                                                            tGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGaaa           >  1:1893277/1‑150 (MQ=255)
                                                                                                                                            tGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAAGCGGGAAAACGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGaaa           >  2:1548177/1‑150 (MQ=255)
                                                                                                                                               gggggaAAGCGATAAAAACTATTCTTGAGGAGTGAGGCGGGCCTTGACCCGAAAGGGGGGAAAGCGACTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGa        <  2:1114873/149‑1 (MQ=255)
                                                                                                                                               cGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGa        >  1:2017074/1‑150 (MQ=255)
                                                                                                                                                gggggAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCAATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGaa       >  1:1337370/1‑150 (MQ=255)
                                                                                                                                                 ggggAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGaaa      >  2:694899/1‑150 (MQ=255)
                                                                                                                                                  gggAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAAc     <  1:4064717/150‑1 (MQ=255)
                                                                                                                                                  gggAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAAc     >  2:1788370/1‑150 (MQ=255)
                                                                                                                                                   ggAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAACg    <  1:498741/150‑1 (MQ=255)
                                                                                                                                                     aaGCGAAAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAACgtt  <  1:2987768/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GACCATTTATGACTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAACGTT  >  AP012306/3903642‑3903940

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑